CN219245557U - Quantitative detection test paper for bovine immunoglobulin G based on quantum dot immunochromatography - Google Patents

Abstract

The utility model relates to the technical field of quantitative detection of bovine immunoglobulin G, and discloses quantitative detection test paper of bovine immunoglobulin G based on quantum dot immunochromatography, which comprises an adhesive backing, water absorbing paper, an NC film, a combining pad and a sample pad, wherein the analysis film is adhered to the middle of the adhesive backing, the water absorbing pad is adhered to one side of the analysis film and forms a first combining area with the analysis film, the combining pad is adhered to the other side of the analysis film and forms a second combining area with the analysis film, the sample pad is adhered to the other side of the combining pad and forms a third combining area with the combining pad, the surface of the analysis film is provided with a detection line and a quality control line, and after experiments, the QD-LF-competition test paper strip can accurately quantify the content of bovine IgG in complex cow milk and a colostrum sample, which cannot be realized by an ultraviolet spectrophotometry; the test strip is very simple to operate, can complete detection in 15 minutes only by three steps of sample dilution, sample loading and machine-readable value loading, and outputs a result without expensive equipment and professional operation places.

Description

Quantitative detection test paper for bovine immunoglobulin G based on quantum dot immunochromatography

Technical Field

The utility model relates to the technical field of quantitative detection of bovine immunoglobulin G, in particular to quantitative detection test paper and a detection method of bovine immunoglobulin G based on quantum dot immunochromatography.

Background

Immunoglobulin (Ig) is a class of immunoglobulins with antibody activity and similar chemical structure to antibodies, which can be combined with antigen to neutralize toxicity, combined with complement to resist invasion of bacteria, viruses and other pathogens, and strengthen the defenses of the organism. Bovine immunoglobulins are widely present in serum and milk, especially in colostrum (which refers to the initial secretion of the mammary gland within 72 hours after bovine production, which is a physiologically abnormal milk), and the main types are IgG, igA and IgM, with IgG accounting for 80%. The detection of the bovine IgG content in the bovine serum or the cow milk is beneficial to evaluating the passive immune effect of calves in a cow farm and guiding the feeding of colostrum, so that the survival rate of the calves is effectively improved and the morbidity is reduced; the method can also provide a powerful product quality supervision means for a dairy for producing milk powder and dairy products; in addition, in the fields of biological pharmacy (vaccine, health care product), biological cosmetics (beauty mask), daily chemicals (novel functional toothpaste) and the like, the detection of the bovine IgG content is also important for evaluating the quality safety of the product. Therefore, the establishment of a rapid, accurate and convenient detection method for the bovine IgG content can be certainly popularized and applied in industries such as livestock veterinarian, dairy products, biological pharmacy, cosmetics, chemicals, standard product production and the like.

Currently, the detection method of the bovine IgG content mainly comprises an ultraviolet spectrophotometry, an immunoassay method and an affinity chromatography method, wherein the immunoassay method mainly comprises an agar diffusion method, an immunoturbidimetry method and an enzyme-linked immunosorbent assay method, and the methods have advantages and disadvantages. The ultraviolet spectrophotometry is simple to operate and low in cost, but is only applicable to analysis of bovine IgG content in standard substances, igG pure products and animal serum samples; while the immunoassay method and the affinity chromatography have accurate analysis and good repeatability, the immunoassay method and the affinity chromatography all need complex instrument and equipment support, have higher implementation cost, complex operation and longer time consumption, and cannot be applied to a base layer.

Disclosure of Invention

The utility model aims to provide quantitative detection test paper and a detection method for bovine immunoglobulin G based on quantum dot immunochromatography, which achieve the aim of realizing quantitative detection of bovine IgG in samples such as bovine serum, bovine milk or bovine dairy products and the like which are integrated into a whole rapidly, simply, accurately and with low cost, and have good application prospects.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the quantitative detection test paper for bovine immunoglobulin G based on quantum dot immunochromatography comprises an immunochromatography test strip, wherein the immunochromatography test strip is respectively a QD-LF-sandwich test strip and a QD-LF-competition test strip;

the immunochromatographic test strip comprises a viscous substrate, an analysis film, a combination pad, a sample pad and water absorption paper, wherein the analysis film is adhered to the middle of the viscous substrate, the water absorption paper is adhered to one side of the analysis film and forms a first combination area with the analysis film, the combination pad is adhered to the other side of the analysis film and forms a second combination area with the analysis film, the sample pad is adhered to the other side of the combination pad and forms a third combination area with the combination pad, and the surface of the analysis film is provided with a detection line and a quality control line.

Preferably, the quality control lines on the surfaces of the analysis membranes of the QD-LF-sandwich test strip and the QD-LF-competition test strip are 2mg/mL goat anti-rabbit polyclonal antibody, the detection line on the surface of the analysis membrane of the QD-LF-sandwich test strip is 3mg/mL rabbit anti-bovine IgG polyclonal antibody, and the detection line on the surface of the analysis membrane of the QD-LF-competition test strip is 2mg/mL bovine IgG.

Preferably, the binding pad of the QD-LF-sandwich test strip uses a QD-labeled rabbit anti-bovine IgG polyclonal antibody as a tracer, and the binding pad of the QD-LF-competition test strip uses a QD-labeled bovine IgG as a tracer.

A quantitative detection method of bovine immunoglobulin G based on quantum dot immunochromatography, comprising the following steps:

step one, extracting bovine IgG;

step two, determining the titer of the bovine IgG immune and rabbit anti-bovine IgG polyclonal antibody;

respectively diluting the goat anti-rabbit antibody, the rabbit anti-bovine IgG polyclonal antibody and the bovine IgG to the spraying concentration by using 0.01 mol/L PB (phosphate buffer solution), spraying a chromatographic membrane, and drying at 37 ℃;

step four, adding 20 mu L of 0.2 g/mL quantum dot into a 1.5mL centrifuge tube, adding 500 mu L of marking buffer solution, placing on a constant temperature oscillator, adding 15 mu L of activating reagent, oscillating for 1h at 1400r/min, adding 0.05mg of rabbit anti-bovine IgG polyclonal antibody or bovine IgG, oscillating for 2h at 25 ℃ at 1400r/min, adding bovine serum albumin with the final concentration of 1%, oscillating for 1h at 1400r/min, adding 0.01 mol/L PB (pH value of 7.0) for 3.88 mL, uniformly mixing, rapidly pouring onto glass fiber by using a capillary tube, drying for 3h at 37 ℃, drying and preserving for later use;

step five, taking chromatography sealing liquid (0.01 mol/L PB containing 1% bovine serum albumin and having a pH value of 7.0) 2 and mL, pouring the mixture onto the cut glass fiber, and drying the glass fiber at 37 ℃ for 2 hours, and drying and preserving the glass fiber for later use;

step six, 2 test strip sensibility, linearity, precision evaluation and field evaluation;

step seven: statistical analysis of the data.

Preferably, the first step is specifically as follows:

60mL of bovine serum is taken and added into a beaker, sodium acetate solution is added dropwise while stirring, and the pH value is regulated to 4.5; dropwise adding 5mL of n-octanoic acid solution, uniformly stirring at normal temperature, placing at 4 ℃ for 1h, transferring into a 50 mL centrifuge tube, centrifuging at 12000r/min for 30min, filtering the supernatant with filter paper, adding 1/10 volume of 0.1moL/L PBS (phosphate buffer solution) into the supernatant, regulating the pH value to 7.0, dropwise adding saturated ammonium sulfate solution to 50% of the total volume while stirring, placing at 4 ℃ for 2h, centrifuging at 12000r/min for 30min, and discarding the supernatant;

resuspension the centrifuged precipitate with 0.01 moL/L PBS, dialyzing at 4deg.C, and changing dialysate 1 time every 2h until ammonia ions are completely removed;

detecting bovine IgG concentration with micro ultraviolet spectrophotometer, and freezing for use.

Preferably, the second step is specifically as follows:

respectively immunizing 2 large-ear white rabbits with prepared bovine IgG antigens in a single subcutaneous injection mode, wherein the immunization period is 1 time for 2 weeks, performing 4 times of immunization, respectively taking the marginal venous blood of 2 large-ear white rabbits after 2 weeks of immunization for 4 times, performing an ELISA (enzyme-linked immunosorbent assay) to detect the rabbit anti-bovine IgG polyclonal antibody titer in 2 parts of serum, stopping immunization when the serum dilution multiple is greater than 10 ten thousand and the absorbance (ODg) of 450nm is greater than 0.200, performing carotid artery blood sampling, wherein the serum dilution multiple is the rabbit anti-bovine IgG polyclonal antibody titer, centrifuging the collected 2 parts of rabbit blood for 15 minutes at 4 ℃ at 5000r/min, and collecting upper-layer serum (serum numbers 1# and 2 #); and (3) extracting rabbit anti-bovine IgG polyclonal antibody in serum by referring to a bovine IgG extraction method, and measuring the concentration and titer of the rabbit anti-bovine IgG polyclonal antibody, and freezing for later use.

Preferably, the sixth step is specifically as follows:

sensitivity evaluation: bovine IgG was gradient diluted with 0.03 mol/L PB to give 0,0.1,0.3,0.5,0.7,1,3,5,7, 10, 30, 50, 70, 100. Mu.g/mL series of concentration standards, which were each tested with 2 strips and repeated 3 times with 0.03 mol/L PB as a blank. The sandwich method test strip takes the standard deviation of +3 times of the average value of blank control detection band signal (T)/quality control band signal (C) as a Cutoff value, and when the T/C value of a sample is more than or equal to the Cutoff value, the content of bovine IgG corresponding to the T/C value of the sample is the detection limit of the sandwich method test strip; the standard deviation of-3 times of the average value of T/C values of blank control is a Cutoff value, and when the T/C value of a sample is less than or equal to the Cutoff value, the concentration of bovine IgG corresponding to the T/C value of the sample is the detection limit of the test strip of the competition method;

linear evaluation: the sandwich method test strip takes lg (T/C value-Cutoff value) as an abscissa, the competition method test strip takes lg (T/C value) as an abscissa, and both test strips draw a quantitative standard curve by taking lg (standard concentration) as an ordinate;

precision evaluation: repeating the test for 3 times for each concentration of the standard to be tested, and calculating a variation Coefficient (CV) of a detection value for 3 times, wherein the smaller the variation coefficient is, the better the precision is;

and (3) field evaluation: and selecting a test strip with better detection performance for field detection, wherein the samples comprise 50 parts of bovine coloctrum (sample numbers 1-50) and 2 parts of pasteurized milk (sample numbers 51 and 52), simultaneously measuring by using a refractometer, randomly extracting 5 parts of bovine coloctrum and 2 parts of pasteurized milk for liquid chromatography measurement, and comparing the correlation of the bovine IgG content detected by 3 methods to evaluate the accuracy of the test strip detection method.

Preferably, the step seven is specifically as follows: sampling Excel software for statistical analysis of test data, respectively drawing quantitative standard curves of 2 test paper detection bovine IgG standards, drawing comparison histograms of bovine IgG content in 52 milk samples detected by a refractometer and an optimal test paper, drawing comparison histograms of bovine IgG content in 7 milk samples detected by the refractometer, the optimal test paper and liquid chromatography, and analyzing correlation of detection results of 3 methods by using the Excel software.

The utility model provides quantitative detection test paper and a detection method for bovine immunoglobulin G based on quantum dot immunochromatography. The beneficial effects are as follows:

(1) The quantum dot immunochromatographic test paper comprises an adhesive base liner, an analysis film, a bonding pad, a sample pad and water absorption paper, wherein the analysis film is adhered to the middle of the adhesive base liner, the water absorption pad is adhered to one side of the analysis film and forms a first bonding area with the analysis film, the bonding pad is adhered to the other side of the analysis film and forms a second bonding area with the analysis film, the sample pad is adhered to the other side of the bonding pad and forms a third bonding area with the bonding pad, and the surface of the analysis film is provided with a detection line and a quality control line. After experiments, the QD-LF-competition test paper strip can accurately quantify the content of bovine IgG in complex cow milk and colostrum samples, which cannot be realized by an ultraviolet spectroscopic method; the test strip is very simple to operate, can complete detection in 15 minutes only by three steps of sample dilution, sample loading and machine-readable value loading, and outputs a result without expensive equipment and professional operation places.

(2) The quantitative detection of bovine IgG in samples such as bovine serum, bovine milk or bovine dairy products and the like, which is integrated with rapidness, simplicity, accuracy and low cost, is truly realized through the obtained quantum dot immunochromatography test strip (competition method), and the method has good application prospect.

Drawings

FIG. 1 is a perspective view of the structure of an immunochromatographic test strip of the present utility model;

FIG. 2 is a structural view of an immunochromatographic test strip of the present utility model;

FIG. 3 is a graph showing the results of the detection of the titers of the rabbit anti-bovine IgG polyclonal antibodies of the present utility model;

FIG. 4 is a graph showing the average T/C values and CV results of the detection of different concentrations of bovine IgG standard by using 2 test strips according to the present utility model;

FIG. 5 is a linear regression equation diagram of 2 test strips of the utility model for detecting bovine IgG standard substances with different concentrations;

FIG. 6 is a diagram of the on-site detection results of a refractometer and QD-LF competition test strips of No. 1-52 milk samples of the utility model;

FIG. 7 is a graph showing correlation results of QD-LF competition test strips, refractometers and liquid chromatography for detecting bovine IgG content in 7 bovine milk samples.

In the figure: 1 adhesive backing, 2 analysis film, 3 binding pad, 4 sample pad, 5 absorbent paper, 6 detection line, 7 quality control line.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Examples of the embodiments are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements throughout or elements having like or similar functionality. The embodiments described below by referring to the drawings are illustrative and intended to explain the present utility model and should not be construed as limiting the utility model.

In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present utility model.

In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

As shown in fig. 1-7, the present utility model provides a technical solution: quantitative detection test paper for bovine immunoglobulin G based on quantum dot immunochromatography, wherein the immunochromatography test paper is respectively a QD-LF-sandwich test paper and a QD-LF-competition test paper;

the quantum dot immunochromatographic test paper comprises a viscous base liner, an analysis film, a combination pad, a sample pad and water absorption paper, wherein the analysis film is attached to the middle of the viscous base liner, the water absorption paper is attached to one side of the analysis film and forms a first combination area with the analysis film, the combination pad is attached to the other side of the analysis film and forms a second combination area with the analysis film, the sample pad is attached to the other side of the combination pad and forms a third combination area with the combination pad, and the surface of the analysis film is provided with a detection line and a quality control line.

The quality control lines 7 on the surfaces of the analysis membranes 2 of the QD-LF-sandwich test strip and the QD-LF-competition test strip are 2mg/mL goat anti-rabbit polyclonal antibody, the detection line on the surface of the analysis membrane 2 of the QD-LF-sandwich test strip is 3mg/mL rabbit anti-bovine IgG polyclonal antibody, and the detection line 6 on the surface of the analysis membrane 2 of the QD-LF-competition test strip is 2mg/mL bovine IgG.

The binding pad 3 of the QD-LF-sandwich test strip takes a QD-labeled rabbit anti-bovine IgG polyclonal antibody as a tracer, and the binding pad 3 of the QD-LF-competition test strip takes a QD-labeled bovine IgG as a tracer.

A quantitative detection method of bovine immunoglobulin G based on quantum dot immunochromatography comprises the following steps:

step one, extracting bovine IgG:

60mL of bovine serum is taken and added into a beaker, sodium acetate solution is added dropwise while stirring, and the pH value is regulated to 4.5; dropwise adding 5mL of n-octanoic acid solution, uniformly stirring at normal temperature, placing at 4 ℃ for 1h, transferring into a 50 mL centrifuge tube, centrifuging at 12000r/min for 30min, filtering the supernatant with filter paper, adding 1/10 volume of 0.1moL/L PBS (phosphate buffer solution) into the supernatant, regulating the pH value to 7.0, dropwise adding saturated ammonium sulfate solution to 50% of the total volume while stirring, placing at 4 ℃ for 2h, centrifuging at 12000r/min for 30min, and discarding the supernatant;

resuspension the centrifuged precipitate with 0.01 moL/L PBS, dialyzing at 4deg.C, and changing dialysate 1 time every 2h until ammonia ions are completely removed;

detecting the concentration of bovine IgG by using a micro ultraviolet spectrophotometer, and freezing for later use;

step two, determining the titer of the bovine IgG immune and rabbit anti-bovine IgG polyclonal antibody:

respectively immunizing 2 large-ear white rabbits with prepared bovine IgG antigens in a single subcutaneous injection mode, wherein the immunization period is 1 time for 2 weeks, performing 4 times of immunization, respectively taking the marginal venous blood of 2 large-ear white rabbits after 2 weeks of immunization for 4 times, performing an ELISA (enzyme-linked immunosorbent assay) to detect the rabbit anti-bovine IgG polyclonal antibody titer in 2 parts of serum, stopping immunization when the serum dilution multiple is greater than 10 ten thousand and the absorbance (ODg) of 450nm is greater than 0.200, performing carotid artery blood sampling, wherein the serum dilution multiple is the rabbit anti-bovine IgG polyclonal antibody titer, centrifuging the collected 2 parts of rabbit blood for 15 minutes at 4 ℃ at 5000r/min, and collecting upper-layer serum (serum numbers 1# and 2 #); extracting rabbit anti-bovine IgG polyclonal antibody in serum by referring to a bovine IgG extraction method, determining the concentration and titer of the rabbit anti-bovine IgG polyclonal antibody, and freezing for later use; the concentrations of the rabbit anti-bovine IgG polyclonal antibodies are 34 mg/mL and 70 mg/mL respectively, and the titers of 2 rabbit anti-bovine IgG polyclonal antibodies detected by an ELISA (enzyme-linked immunosorbent assay) are 1: 102 400, and the results are shown in FIG. 3.

Respectively diluting the goat anti-rabbit antibody, the rabbit anti-bovine IgG polyclonal antibody and the bovine IgG to the spraying concentration by using 0.01 mol/L PB (phosphate buffer solution), spraying a chromatographic membrane, and drying at 37 ℃;

step four, adding 20 mu L of 0.2 g/mL quantum dot into a 1.5mL centrifuge tube, adding 500 mu L of marking buffer solution, placing on a constant temperature oscillator, adding 15 mu L of activating reagent, oscillating for 1h at 1400r/min, adding 0.05mg of rabbit anti-bovine IgG polyclonal antibody or bovine IgG, placing at 25 ℃, oscillating for 2h at 1400r/min, adding bovine serum albumin with the final concentration of 1%, oscillating for 1h at 1400r/min, adding 3.88 mL of 0.01 mol/L PB (pH value of 7.0), uniformly mixing, rapidly pouring onto glass fiber by using a capillary tube, drying for 3h at 37 ℃, drying and preserving for later use;

step five, taking chromatography sealing liquid (0.01 mol/L PB containing 1% bovine serum albumin and having a pH value of 7.0) 2 and mL, pouring the mixture onto the cut glass fiber, and drying the glass fiber at 37 ℃ for 2 hours, and drying and preserving the glass fiber for later use;

step six, 2 test strip sensibility, linearity, precision evaluation and field evaluation

Sensitivity evaluation: bovine IgG was gradient diluted with 0.03 mol/L PB to give 0,0.1,0.3,0.5,0.7,1,3,5,7, 10, 30, 50, 70, 100. Mu.g/mL series of concentration standards, which were each tested with 4 strips and repeated 3 times with 0.03 mol/L PB as a blank. The sandwich method test strip takes the standard deviation of +3 times of the average value of blank control detection band signal (T)/quality control band signal (C) as a Cutoff value, and when the T/C value of a sample is more than or equal to the Cutoff value, the content of bovine IgG corresponding to the T/C value of the sample is the detection limit of the sandwich method test strip; the standard deviation of-3 times of the average value of T/C values of blank control is a Cutoff value, and when the T/C value of a sample is less than or equal to the Cutoff value, the concentration of bovine IgG corresponding to the T/C value of the sample is the detection limit of the test strip of the competition method;

linear evaluation: the sandwich method test strip takes lg (T/C value-Cutoff value) as an abscissa, the competition method test strip takes lg (T/C value) as an abscissa, and both test strips draw a quantitative standard curve by taking lg (standard concentration) as an ordinate;

precision evaluation: repeating the test for 3 times for each concentration of the standard to be tested, and calculating a variation Coefficient (CV) of a detection value for 3 times, wherein the smaller the variation coefficient is, the better the precision is;

QD-LF-sandwich and QD-LF-competition test strips sensitivity, linearity, precision results:

the Cutoff values of the 2 test strips are 0.635,2.864, and the detection limits are 0.1 mug/mL and 0.5 mug/mL respectively. CV of T/C values for standards at each concentration is shown in FIG. 4. The T/C value-Cutoff value of the test strip detection standard by the sandwich method is positively correlated with the concentration of the standard, and the T/C value of the test strip detection standard by the competition method is negatively correlated with the concentration of the standard, as shown in figure 5. The QD-LF-competition method test strip has better precision, higher sensitivity, wider linear range and better performance.

And (3) field evaluation: selecting a competition test strip with better detection performance for field detection, wherein the samples comprise 50 parts of bovine coloctrum (sample numbers 1-50) and 2 parts of pasteurized milk (sample numbers 51 and 52), simultaneously measuring by using a refractometer, randomly extracting 5 parts of bovine coloctrum and 2 parts of pasteurized milk for liquid chromatography measurement, and comparing the correlation of the bovine IgG content detected by 3 methods to evaluate the accuracy of the test strip detection method; 50 parts of bovine coloctrum and 2 parts of pasteurized milk are detected by a refractometer and a QD-LF-competition test strip on site, the detection result of 50 parts of bovine coloctrum is shown that the detection result of the refractometer is concentrated to 20-80 mg/mL, the detection result of the QD-LF-competition method is 50-230 mg/mL, the result is shown in figure 6, 5 parts of bovine coloctrum and 2 parts of pasteurized milk samples are extracted for liquid chromatography, the detection data of 3 methods of 7 samples are analyzed, the correlation between the QD-LF-competition test strip and the liquid chromatography detection value is up to 0.9759, and the correlation between the refractometer and the liquid chromatography detection result is only 0.8274, which is shown in figure 7.

Step seven: statistical analysis of data

Sampling Excel software for statistical analysis of test data, respectively drawing quantitative standard curves of 2 test paper detection bovine IgG standards, drawing comparison histograms of bovine IgG content in 52 milk samples detected by a refractometer and an optimal test paper, drawing comparison histograms of bovine IgG content in 7 milk samples detected by the refractometer, the optimal test paper and liquid chromatography, and analyzing correlation of detection results of 3 methods by using the Excel software.

In conclusion, the quantum dot immunochromatography test strip (competition method) obtained by the test truly realizes the quantitative detection of bovine IgG in samples such as bovine serum, bovine milk or bovine dairy products, which integrates the advantages of rapidness, simplicity, accuracy and low cost, and has good application prospect.

It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present utility model, and the present utility model is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present utility model has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims (3)

1. The quantitative detection test paper for bovine immunoglobulin G based on quantum dot immunochromatography comprises Quantum Dot (QD) immunochromatography test paper and is characterized in that: the immunochromatography test paper is respectively a QD-LF-sandwich test paper and a QD-LF-competition test paper;

the immunochromatographic test paper comprises an adhesive base lining (1), an analysis film (2), a bonding pad (3), a sample pad (4) and water absorbing paper (5), wherein the analysis film (2) is attached to the middle of the adhesive base lining (1), the water absorbing paper (5) is attached to one side of the analysis film (2) and forms a first bonding area with the analysis film (2), the bonding pad (3) is attached to the other side of the analysis film (2) and forms a second bonding area with the analysis film (2), the sample pad (4) is attached to the other side of the bonding pad (3) and forms a third bonding area with the bonding pad (3), and a detection line (6) and a quality control line (7) are arranged on the surface of the analysis film (2).

2. The quantitative detection test paper for bovine immunoglobulin G based on quantum dot immunochromatography according to claim 1, which is characterized in that: the quality control lines (7) on the surfaces of the analysis membranes (2) of the QD-LF-sandwich test strip and the QD-LF-competition test strip are 2mg/mL goat anti-rabbit polyclonal antibodies, the detection lines (6) on the surfaces of the analysis membranes (2) of the QD-LF-sandwich test strip are 3mg/mL rabbit anti-bovine IgG polyclonal antibodies, and the detection lines (6) on the surfaces of the analysis membranes (2) of the QD-LF-competition test strip are 2mg/mL bovine IgG.

3. The quantitative detection test paper for bovine immunoglobulin G based on quantum dot immunochromatography according to claim 1, which is characterized in that: the binding pad (3) of the QD-LF-sandwich test strip takes a QD-labeled rabbit anti-bovine IgG polyclonal antibody as a tracer, and the binding pad (3) of the QD-LF-competition test strip takes a QD-labeled bovine IgG as a tracer.

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