CN219224545U - Multichannel fluorescence detection system - Google Patents

Abstract

The utility model discloses a multichannel fluorescence detection system, which comprises: the device comprises a fixing seat, a plurality of excitation light assemblies and an imaging device, wherein the fixing seat is provided with a first mounting hole and a plurality of second mounting holes, the first mounting hole is formed in the middle of the fixing seat, and the plurality of second mounting holes are arranged at intervals along the circumferential direction of the first mounting hole; the excitation light assemblies are arranged in the second mounting holes and correspond to the second mounting holes one by one, and are respectively and electrically connected with the control device and used for exciting the fluorescence of the chip; the imaging device comprises an optical filter, wherein the optical filter is arranged in the first mounting hole, and the central axis of the second mounting hole extends obliquely relative to the central axis of the first mounting hole. According to the multichannel fluorescence detection system provided by the embodiment of the utility model, the fluorescence detection effect can be improved by independently arranging the excitation light component in each second mounting hole, and the rapid switching of the fluorescence channel can be realized by electrically connecting the excitation light component with the control device, so that the fluorescence collection efficiency is improved.

Description

Multichannel fluorescence detection system

Technical Field

The utility model relates to the technical field of digital PCR, in particular to a multichannel fluorescence detection system.

Background

In the prior art, the DPCR illumination light path device has large volume, the switching of different fluorescent channels is realized by driving a motion mechanism, the operation steps are complicated, the collection efficiency of fluorescence is influenced, the obvious fluorescence crosstalk phenomenon exists between different fluorescent channels, the detection effect of fluorescence is influenced, and the improvement space exists.

Disclosure of Invention

The present utility model aims to solve at least one of the technical problems existing in the prior art. Therefore, an object of the present utility model is to provide a multi-channel fluorescence detection system, which has high fluorescence collection efficiency and good fluorescence detection effect.

A multichannel fluorescence detection system according to an embodiment of the utility model includes: the device comprises a fixing seat, a plurality of excitation light assemblies and an imaging device, wherein the fixing seat is provided with a first mounting hole and a plurality of second mounting holes, the first mounting hole is formed in the middle of the fixing seat, and the second mounting holes are arranged at intervals along the circumferential direction of the first mounting hole; the excitation light assemblies are arranged in the second mounting holes and correspond to the second mounting holes one by one, the excitation light assemblies are respectively and electrically connected with the control device, and the excitation light assemblies are used for exciting fluorescence of the chip; the imaging device comprises an optical filter, and the optical filter is arranged in the first mounting hole, wherein the central axis of the second mounting hole extends obliquely relative to the central axis of the first mounting hole.

According to the multichannel fluorescence detection system provided by the embodiment of the utility model, the excitation light assemblies are independently arranged in each second mounting hole, so that each fluorescence channel is provided with an independent light source, the crosstalk phenomenon between different fluorescence channels is avoided, the fluorescence detection effect is improved, the excitation light assemblies are electrically connected with the control device, the excitation light assemblies can be switched to emit light through the control device, the rapid switching of the fluorescence channels is realized, the collection of multiple fluorescence is realized, no additional movement mechanism is required, the operation steps are simplified, the fluorescence collection efficiency is improved, and meanwhile, the occupied space is saved; the filter is arranged in the first mounting hole, so that stray light signals can be filtered, the influence of the stray light signals on the acquisition of fluorescent signals is reduced, and the fluorescent detection effect is improved; through arranging the axis of second mounting hole for the axis slope of first mounting hole, the excitation light subassembly is shone the excitation light slope to the chip on, and the fluorescence excitation volume of multiplicable chip makes every excitation light subassembly and light filter cooperation form the fluorescence passageway simultaneously, reduces the crosstalk phenomenon between the different fluorescence passageways, improves fluorescence detection effect, guarantees that the fluorescence of chip will be emitted to the light filter after receiving the excitation of excitation light, guarantees fluorescence collection volume, improves fluorescence detection effect.

According to some embodiments of the utility model, an angle α between a central axis of the second mounting hole and a central axis of the first mounting hole is 25 ° or more and 35 ° or less.

According to some embodiments of the utility model, the fixing base comprises a fixing platform and an annular boss, the first mounting hole is formed in the middle of the fixing platform, the annular boss is arranged on the periphery of the fixing platform and provided with the second mounting hole, and the annular boss extends obliquely upwards in a direction away from the fixing platform.

According to some embodiments of the present utility model, two excitation light components located opposite to each other in the aperture direction of the first mounting hole form a group of excitation light, and the light emitting parts of the two excitation light components of each group of excitation light emit light beams with the same wavelength band.

According to some embodiments of the utility model, the excitation light assembly includes a light emitting member, a first lens, and a second lens arranged in sequence, the light emitting member, the first lens, and the second lens are arranged at intervals in sequence along a central axis of the second mounting hole, and a center of the light emitting member, a center of the first lens, and a center of the second lens are located on the central axis of the second mounting hole.

In some examples, the multichannel fluorescence detection system further comprises a heat dissipation device, the light emitting element is arranged on the circuit board, and the heat dissipation device is arranged on the fixing seat and is in abutting fit with the circuit board.

In some examples, the heat dissipating device includes a heat dissipating plate, wherein a side of the heat dissipating plate facing the circuit board has a plurality of heat dissipating bosses, the heat dissipating bosses are abutted to the circuit board, and a side of the heat dissipating plate facing away from the circuit board is provided with a plurality of heat dissipating fins.

In some examples, the imaging device further comprises a lens and a vision camera, wherein the optical filter is arranged at one end of the lens, and the vision camera is arranged at the other end of the lens, and the central axis of the lens coincides with the central line of the first mounting hole.

In some examples, the lens includes a macro lens and a liquid lens.

In some examples, the first mounting hole has a hole diameter b, where 35 mm.ltoreq.b.ltoreq.40 mm.

Additional aspects and advantages of the utility model will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the utility model.

Drawings

The foregoing and/or additional aspects and advantages of the utility model will become apparent and may be better understood from the following description of embodiments taken in conjunction with the accompanying drawings in which:

FIG. 1 is a schematic diagram of a multi-channel fluorescence detection system according to an embodiment of the present utility model;

FIG. 2 is a schematic diagram illustrating the operation of a multi-channel fluorescence detection system according to an embodiment of the present utility model;

FIG. 3 is a schematic diagram of the installation of a multi-channel fluorescence detection system according to an embodiment of the present utility model;

FIG. 4 is a schematic view of a fixing base according to an embodiment of the present utility model;

FIG. 5 is a schematic view of a fixing base according to an embodiment of the present utility model in another view;

FIG. 6 is a schematic diagram of an excitation light component according to an embodiment of the utility model;

FIG. 7 is a schematic diagram of the operation of an excitation light assembly according to an embodiment of the utility model;

fig. 8 is a schematic structural view of a heat dissipating device according to an embodiment of the present utility model.

Reference numerals:

the multi-channel fluorescence detection system 100, chip 200,

a fixing base 10, a fixing platform 11, a first mounting hole 111, an annular boss 12, a second mounting hole 121,

an excitation light assembly 20, a first lens 21, a second lens 22, a light emitting member 23,

an imaging device 30, a lens 31, a macro lens 311, a liquid lens 312, a vision camera 32, a filter 33,

heat sink 40, heat sink 41, heat dissipating boss 42, heat dissipating fin 43,

the dimensions of the circuit board 50, the boss 501,

and a lamp holder 60.

Detailed Description

Embodiments of the present utility model are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the utility model.

In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present utility model. Furthermore, features defining "first", "second" may include one or more such features, either explicitly or implicitly. In the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

A multichannel fluorescence detection system 100 according to an embodiment of the present utility model is described below with reference to fig. 1-8.

As shown in fig. 1 to 8, a multi-channel fluorescence detection system 100 according to an embodiment of the present utility model includes: the light source comprises a fixing base 10, a plurality of excitation light assemblies 20 and an imaging device 30, wherein the fixing base 10 is provided with a first mounting hole 111 and a plurality of second mounting holes 121, the first mounting hole 111 is arranged in the middle of the fixing base 10, and the plurality of second mounting holes 121 are arranged at intervals along the circumferential direction of the first mounting hole 111; the excitation light component 20 is disposed in the second mounting hole 121, the excitation light component 20 is used for exciting fluorescence of the chip 200, the imaging device 30 comprises a light filter 33, the light filter 33 is disposed in the first mounting hole 111, specifically, light emitted by the excitation light component 20 irradiates the chip 200 through the second mounting hole 121, so that the chip 200 is excited to emit fluorescence, and part of the fluorescence can pass through the first mounting hole 111 along the top-to-bottom direction and is incident on the imaging device 30 to realize fluorescence collection; the optical filter 33 can filter the light beam with a specific wavelength band, so that the narrow-band light beam in the fluorescence is incident on the imaging device 30 to filter the stray light signal, reduce the influence of the stray light signal on the acquisition of the fluorescence signal, and improve the fluorescence detection effect.

The central axis of the second mounting hole 121 extends obliquely relative to the central axis of the first mounting hole 111, the chip 200 may be disposed at a position opposite to the optical filter 33, the chip 200 is parallel to the optical filter 33, the light emitted by the excitation light component 20 may be irradiated onto the chip 200 at a certain oblique angle, and after the excitation light of the chip 200 is excited, the fluorescence is emitted to the optical filter 33, so that the flux of the fluorescence entering the first mounting hole 111 may be increased, and the fluorescence collection amount may be further increased, thereby improving the fluorescence detection effect.

The excitation light assemblies 20 in the plurality of second mounting holes 121 can be respectively matched with the optical filters 33 in the first mounting holes 111 to form a plurality of fluorescence channels, and the plurality of excitation light assemblies 20 are in one-to-one correspondence with the plurality of second mounting holes 121, so that each fluorescence channel is provided with an independent light source, the crosstalk phenomenon among different fluorescence channels can be avoided, and the fluorescence detection effect can be improved; the excitation light components 20 are respectively electrically connected with the control device, the control device can control the excitation light components 20 to independently work and emit light, so that the rapid switching of a fluorescent channel can be realized, the excitation light components 20 or the fixing seat 10 are not required to be driven to rotate, the operation steps are simplified, the fluorescent collection efficiency is improved, the number of parts of the detection system can be reduced, and the overall structure size is reduced.

According to the multi-channel fluorescence detection system 100 of the embodiment of the utility model, by arranging the excitation light assemblies 20 in each second mounting hole 121 independently, each fluorescence channel can be provided with an independent light source, so that the crosstalk phenomenon between different fluorescence channels can be avoided, the fluorescence detection effect is improved, and the excitation light assemblies 20 are electrically connected with the control device, and the control device can switch the excitation light assemblies 20 to emit light, so that the rapid switching of the fluorescence channels is realized, thereby realizing the collection of multiple fluorescence without arranging a motion mechanism additionally, simplifying the operation steps, improving the fluorescence collection efficiency, and saving the occupied space; by arranging the optical filter 33 in the first mounting hole 111, stray light signals can be filtered, the influence of the stray light signals on the acquisition of fluorescent signals is reduced, and the fluorescent detection effect is improved; through arranging the central axis of the second mounting hole 121 obliquely relative to the central axis of the first mounting hole 111, the excitation light assemblies 20 obliquely irradiate the excitation light onto the chip 200, so that the fluorescence excitation amount of the chip 200 can be increased, each excitation light assembly 20 and the optical filter 33 are matched to form a fluorescence channel, the crosstalk phenomenon between different fluorescence channels is reduced, the fluorescence detection effect is improved, the fluorescence of the chip 200 is ensured to be emitted to the optical filter 33 after being excited by the excitation light, the fluorescence collection amount is ensured, and the fluorescence detection effect is improved.

As shown in fig. 1, fig. 2 and fig. 3, according to some embodiments of the present utility model, the included angle between the central axis of the second mounting hole 121 and the central axis of the first mounting hole 111 is α, when α is too large, the size of the fixing base 10 needs to be increased, so that the whole device occupies a larger space, when α is too small, the excitation light emitted by the excitation light component 20 to the chip 200 is easy to interfere with the light emitted by the fluorescence emitted to the optical filter 33, which affects the imaging effect, and further, the value range of α may be limited to be 25 ° or less than or equal to 35 °, specifically, α may be 25 ° or 35 °, and α may also be any value between 25 ° and 35 °, for example, α may be 28 °, 30 ° and 32 °, etc., so that the excitation light emitted by the excitation light component 20 is prevented from interfering with the fluorescence emitted by the chip 200, the fluorescence collection effect is improved, the product is convenient to be miniaturized, the use experience is improved, preferably, and the value of α is 30 ° so that the chip 200 is excited to irradiate stably the fluorescence, which is favorable for the imaging device 30 to stably detect the fluorescence.

As shown in fig. 1, fig. 4 and fig. 5, according to some embodiments of the present utility model, the fixing base 10 includes a fixing platform 11 and an annular boss 12, a first mounting hole 111 is formed in the middle of the fixing platform 11, the annular boss 12 is disposed at the periphery of the fixing platform 11, and the annular boss 12 has a second mounting hole 121, and the annular boss 12 extends obliquely upward along a direction away from the fixing platform 11, so that the second mounting hole 121 with a central axis inclined from a horizontal plane is disposed at the periphery of the first mounting hole 111, thereby facilitating the installation of the excitation light assembly 20, and light emitted by the excitation light assembly 20 in the second mounting hole 121 can be obliquely irradiated to the chip 200, so that a spot area on the surface of the chip 200 is increased, the surface of the chip 200 is uniformly irradiated, and further, the fluorescence excitation amount on the surface of the chip 200 is improved, and the fluorescence collection effect is improved.

As shown in fig. 2, according to some embodiments of the present utility model, two excitation light assemblies 20 located in the aperture direction of the first mounting hole 111 and opposite to each other form a group of excitation light, and the light emitting parts 23 of the two excitation light assemblies 20 of each group of excitation light emit light beams with the same wavelength bands, and the two excitation light assemblies 20 opposite to each other are opposite to each other through a certain inclination angle to the chip 200, so that the light intensity of the illumination area can be ensured to be uniform, the light receiving of the chip 200 can be uniform, the fluorescence excitation amount on the surface of the chip 200 can be improved, and the fluorescence collection effect can be improved.

As shown in fig. 4 and 5, in some examples, the outer circumference of the annular boss 12 has a lamp holder 60, which is convenient for an operator to hold, and the lamp holder 60 may be penetrated by a screw or a fastener such as a screw to fix the multi-channel fluorescence detection system 100, so that the stability of fluorescence detection may be improved; the lamp holder 60 can be integrally formed with the annular boss 12, the lamp holder 60 forms a lug located at the outer side of the annular boss 12, and the lug is provided with a through hole for a screw or a fastener such as a screw to pass through.

As shown in fig. 3, 6 and 7, according to some embodiments of the present utility model, the excitation light assembly 20 includes a light emitting member 23, a first lens 21 and a second lens 22 sequentially arranged, the light emitting member 23, the first lens 21 and the second lens 22 are sequentially arranged at intervals along a central axis of the second mounting hole 121, and a center of the light emitting member 23, a center of the first lens 21 and a center of the second lens 22 are positioned on the central axis of the second mounting hole 121, so that an imaging effect can be ensured to stably excite fluorescence of the chip 200.

It is understood that the first lens 21 may be a convex lens for compressing the divergence angle of the light beam to collect the light beam, so as to ensure the luminous efficiency of the excitation light assembly 20; the second lens 22 may be a convex lens, and is used for imaging, so as to ensure the size of the emergent light spot, that is, the size of the light spot on the chip 200, so as to stably excite the chip 200 to emit fluorescence, and improve the fluorescence collection effect.

As shown in fig. 7, in some examples, the excitation light component 20 is defocused by 10mm near the imaging position by adopting a critical illumination manner, that is, the focal point of the second lens 22 may be located at a position 10mm above the chip 200, so that the light emitted by the light emitting element 23 is imaged on the surface of the chip 200 in advance, the area of the imaging light spot may be increased, and then the target area is uniformly illuminated, so as to stably excite the chip 200 to emit fluorescence, and the fluorescence collection effect is improved.

It should be noted that, the light emitting element 23 may be an LED lamp, the light emitting effect is good, the cost is low, the imaging shape of the light emitted by the filament in the LED lamp at the focal point of the second lens 22 is a strip-shaped light band, if the focal point is located on the chip 200, the chip 200 is unevenly illuminated, the stability of exciting the fluorescence of the chip 200 is easily affected, the fluorescence collection effect is affected, the area of the imaging light spot at the surface of the chip 200 can be increased by defocusing imaging onto the chip 200, and then the target area is uniformly illuminated, so as to stably excite the fluorescence of the chip 200, and the fluorescence collection effect is improved.

As shown in fig. 2, 3 and 8, in some examples, the multi-channel fluorescence detection system 100 further includes a heat dissipating device 40, the light emitting element 23 is disposed on the circuit board 50, the circuit board 50 may be connected with the fixing base 10, the heat dissipating device 40 may be disposed on the fixing base 10, so as to fix the heat dissipating device 40, and the heat dissipating device 40 is in abutting engagement with the circuit board 50, so that the heat generated by the operation of the circuit board 50 and the light emitting element 23 is led out by the heat dissipating device 40 to dissipate heat, and normal operation of the circuit board 50 and the light emitting element 23 is ensured.

As shown in fig. 1 and 8, in some examples, the heat dissipating device 40 includes a heat dissipating plate 41, one side of the heat dissipating plate 41 facing the circuit board 50 has a plurality of heat dissipating bosses 42, the heat dissipating bosses 42 are abutted against the circuit board 50, so that the heat dissipating device 40 is directly contacted with the circuit board 50, and the circuit board 50 is uniformly cooled, a plurality of heat dissipating fins 43 are disposed on one side of the heat dissipating plate 41 facing away from the circuit board 50, so that the heat dissipating area can be increased, and the heat dissipating fins 43 are arranged at intervals, so that natural heat dissipation by air convection is utilized, specifically, heat of the light emitting element 23 and the circuit board 50 can be thermally conducted to the heat dissipating plate 41 through the heat dissipating bosses 42, and then conducted to the heat dissipating fins 43, so that the heat dissipating fins 43 dissipate the heat to the outside, thereby realizing heat dissipation and cooling of the circuit board 50 and the light emitting element 23 by the heat dissipating device 40, and the heat dissipating effect can be guaranteed, and the work effect of the circuit board 50 can be improved, and the light emitting effect of the excitation light assembly 20 can be improved.

As shown in fig. 1, in some examples, the outer side of the circuit board 50 has two protrusions 501 to facilitate wiring to connect the circuit board 50 to the positive and negative electrodes, thereby making electrical connection of the excitation light assembly 20 to the control device.

As shown in fig. 8, in some examples, the heat dissipating plate 41 is provided with a plurality of holes, the holes extend along the up-down direction, and the inner walls of the holes are provided with threads, so that the fasteners can be conveniently penetrated to realize the fixed connection between the heat dissipating device 40 and the fixing base 10, wherein the fasteners can be screws, bolts or screws, etc., so that the operation is convenient, and the cost is low.

As shown in fig. 1, 2 and 3, in some examples, the imaging device 30 further includes a lens 31 and a vision camera 32, the optical filter 33 is disposed at an upper end of the lens 31, and the vision camera 32 is disposed at a lower end of the lens 31, where a central axis of the lens 31 coincides with a central axis of the first mounting hole 111, and fluorescent light is required to be incident into the imaging device 30 through the first mounting hole 111, so that the lens 31 is convenient to receive and collect fluorescent light, and a fluorescent light collecting effect can be improved.

In some examples, the vision camera 32 may be an SCMOS camera, which has the characteristics of high sensitivity, high resolution, large field of view, high frame rate, low noise, and the like, and has good observation effect on objects with different transparency, and fluorescent signals can be imaged to the vision camera 32 through the lens 31, so as to complete collection of the fluorescent signals.

As shown in fig. 1, in some examples, the lens 31 includes a macro lens 311 and a liquid lens 312, where the macro lens 311 is a fixed focus lens 31 for capturing very fine objects, and the liquid lens 312 can automatically capture a picture in a focal range to form a smooth and clear image; the macro lens 311 is located at the upper part of the liquid lens 312, the macro lens 311 and the liquid lens 312 are combined to form a low-power imaging optical system, the imaging effect of large depth of field and large field can be achieved, and the liquid lens 312 is electrically connected with the control device to automatically focus, so that the requirements of the processing precision and the positioning precision of the chip 200 are reduced.

As shown in fig. 2, in some examples, the distance between the center of the chip 200 and the excitation light assembly 20 is a, the included angle between the central axis of the second mounting hole 121 and the central axis of the first mounting hole 111 is α, the distance between the center of the chip 200 and the upper end of the lens 31 is a certain value, so that the operation steps can be simplified, and the adjustment of operators is facilitated under the condition of guaranteeing the fluorescence collection effect, and therefore, when a is 30 °, for example, a is equal to 78.00mm, the fluorescence excitation amount on the surface of the chip 200 can be kept stable, which is beneficial to the imaging device 30 to collect fluorescence stably, and further, the fluorescence detection effect can be improved, the size of the fixing seat 10 can be reduced, the miniaturization of products is facilitated, and the use experience is improved.

As shown in fig. 4, in some examples, the aperture of the first mounting hole 111 is b, when b is too large, the first mounting hole 111 is easy to pass through excessive stray light signals, so as to affect the fluorescence detection effect, when b is too small, the fluorescence flux of the first mounting hole 111 is reduced, so as to affect the fluorescence collection effect, and further, the value range of b can be limited to be 35mm less than or equal to b less than or equal to 40mm, specifically, b can be 35mm or 40mm, b can be any numerical value between 35mm and 40mm, for example, b can be 37mm, 38mm, 39mm, and the like, so that the fluorescence flux at the first mounting hole 111 is improved, the fluorescence collection effect is improved, the stray light signals can be effectively filtered by the optical filter 23, the fluorescence detection effect is improved, preferably, the value of b is 35mm, the fluorescence detection effect can be stabilized, and sufficient space can be reserved for the upper end of the lens 31.

In some examples, the chip 200 may be a biochip 200, and the biochip 200 may be coated with multiple fluorescent dyes, the different fluorescent dyes may be excited to emit fluorescence in different light beam bands, and by switching the multiple groups of excitation light assemblies 20 to emit light to irradiate the chip 200, the multiple fluorescent dyes may be excited to emit fluorescence respectively, so as to improve fluorescence detection efficiency.

It can be understood that if the light beam band emitted by the excitation light component 20 coincides with the light beam band required by exciting multiple fluorescent dyes to emit fluorescence, the multiple fluorescent dyes can be excited to enter the first mounting hole 111 at the same time, and the fluorescence can be filtered to form a narrow-band light beam by arranging the optical filter 33 in the first mounting hole 111, so that only one fluorescent dye is excited to be incident to the imaging device 30, the accuracy of fluorescence detection is improved, the fluorescence detection effect is further improved, and the volume of the multi-channel fluorescence detection system 100 can be compressed, which is beneficial to miniaturization of products and improvement of use experience.

Other configurations and operations of the multi-channel fluorescence detection system 100 according to embodiments of the present utility model are known to those of ordinary skill in the art and will not be described in detail herein. In the description of the utility model, a "first feature" or "second feature" may include one or more of such features. The vertical direction, the horizontal direction, and the front-rear direction are defined by the vertical direction, the horizontal direction, and the front-rear direction in the drawing.

In the description of the present utility model, unless explicitly stated and limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, or may include both the first and second features not being in direct contact but being in contact by another feature therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature.

In the description of the present specification, reference to the terms "one embodiment," "some embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

While embodiments of the present utility model have been shown and described, it will be understood by those of ordinary skill in the art that: many changes, modifications, substitutions and variations may be made to the embodiments without departing from the spirit and principles of the utility model, the scope of which is defined by the claims and their equivalents.

Claims (10)

1. A multi-channel fluorescence detection system, comprising:

the fixing seat is provided with a first mounting hole and a plurality of second mounting holes, the first mounting hole is arranged in the middle of the fixing seat, and the plurality of second mounting holes are arranged at intervals along the circumferential direction of the first mounting hole;

the excitation light assemblies are arranged in the second mounting holes and correspond to the second mounting holes one by one, the excitation light assemblies are respectively and electrically connected with the control device, and the excitation light assemblies are used for exciting fluorescence of the chip;

the imaging device comprises an optical filter which is arranged in the first mounting hole,

the central axis of the second mounting hole extends obliquely relative to the central axis of the first mounting hole.

2. The multi-channel fluorescence detection system of claim 1, wherein an angle between a central axis of the second mounting hole and a central axis of the first mounting hole is α, wherein α is 25 ° and 35 °.

3. The multi-channel fluorescence detection system of claim 1, wherein the fixing base comprises a fixing platform and an annular boss, the first mounting hole is formed in the middle of the fixing platform, the annular boss is formed in the periphery of the fixing platform and provided with the second mounting hole, and the annular boss extends in an upward inclined mode in a direction away from the fixing platform.

4. The multi-channel fluorescence detection system of claim 1, wherein two of the excitation light components located opposite to each other in the aperture direction of the first mounting hole form a group of excitation light, and the light emitting members of the two excitation light components of each group of excitation light emit light beams with the same wavelength band.

5. The multi-channel fluorescence detection system of claim 1, wherein the excitation light assembly comprises a light emitting member, a first lens, and a second lens arranged in sequence, the light emitting member, the first lens, and the second lens are arranged in sequence at intervals along a central axis of the second mounting hole, and a center of the light emitting member, a center of the first lens, and a center of the second lens are located on the central axis of the second mounting hole.

6. The multi-channel fluorescence detection system of claim 5, further comprising a heat sink, wherein the light emitting element is disposed on the circuit board, and wherein the heat sink is disposed on the fixing base and is in abutting engagement with the circuit board.

7. The multi-channel fluorescence detection system of claim 6, wherein the heat dissipating device comprises a heat dissipating plate, a plurality of heat dissipating bosses are arranged on one side of the heat dissipating plate facing the circuit board, the heat dissipating bosses are abutted to the circuit board, and a plurality of heat dissipating fins are arranged on one side of the heat dissipating plate facing away from the circuit board.

8. The multi-channel fluorescence detection system of any one of claims 1-7, wherein the imaging device further comprises a lens and a vision camera, the optical filter is disposed at one end of the lens, the vision camera is disposed at the other end of the lens, and wherein a central axis of the lens coincides with a central axis of the first mounting hole.

9. The multi-channel fluorescence detection system of claim 8, wherein the lens comprises a macro lens and a liquid lens.

10. The multi-channel fluorescence detection system of any of claims 1-7, wherein the first mounting hole has a hole diameter b, wherein 35mm ∈b ∈40mm.

Images