CN219217993U - Elution structure and separation device - Google Patents

Abstract

An elution structure for eluting a target deposited on one side of a filter membrane includes a jet member, a carrier member, and an elution chamber. The jet member is used for emitting the eluent. The current carrying piece comprises a current carrying pipeline and a leakage fluid arranged in the current carrying pipeline, wherein the leakage fluid comprises a leakage surface which is obliquely arranged. The elution cavity comprises a first part, a second part and a third part which are communicated with the current carrying pipeline, the second part is communicated between the first part and the third part, the second part is used for arranging a filter membrane, the other end of the leakage flow surface is used for being connected with one end of the filter membrane to guide eluent to the top end of the filter membrane, the eluent is used for eluting a target object deposited on the filter membrane and forming suspension, the first part is used for accommodating the suspension, and the third part is used for being connected with an external environment to discharge redundant eluent. The elution structure provided by the application can improve the elution efficiency and reduce the damage of the target object. In addition, the application also provides a separation device.

Description

Elution structure and separation device

Technical Field

The application relates to the technical field of exosome separation, in particular to an elution structure and a separation device for flushing membranous vesicles deposited on a filter membrane.

Background

Exosome (Exosome) separation devices are mainly based on size exclusion to entrap membranous vesicles (Membrane Vesicles) on the nanofiltration membrane surface and to filter out contaminants. When the separation of the membrane vesicles is completed, the membrane vesicles deposit on the surface of the nanofiltration membrane to form a layer of relatively stable/firm colloid-like substance, which needs to be re-dispersed into the solution to form a suspension for subsequent use or preservation of the membrane vesicles.

For redispersing the membrane vesicles deposited on the nanofiltration membrane into a solution, the existing practice is mainly to suck a proper amount of solution through a pipette, and then manually wash the nanofiltration membrane loaded with the membrane vesicles for a plurality of times to elute the membrane vesicles and form a suspension. However, this approach is prone to damage to the membranous vesicles and manual rinsing is cumbersome, resulting in poor quality stability of the final suspension.

Disclosure of Invention

In view of the above, it is necessary to provide an elution structure capable of solving the above-described problems.

In addition, it is also necessary to provide a separation device having the above elution structure.

An elution structure for eluting a target deposited on one side of a filter membrane, the elution structure comprising:

the jet piece is used for jetting the eluent;

the current carrying piece comprises a current carrying pipeline and a leakage fluid arranged in the current carrying pipeline, wherein the leakage fluid comprises a leakage surface which is obliquely arranged, and one end of the leakage surface is connected with the inner wall of the current carrying pipeline;

the elution cavity is connected with the current carrying piece and comprises a first part, a second part and a third part which are communicated with the current carrying pipeline, the second part is communicated between the first part and the third part, the second part is used for arranging the filter membrane, the other end of the drainage surface is used for being connected with one end of the filter membrane so as to guide the eluent to the top end of the filter membrane, the eluent is used for eluting the target object deposited on the filter membrane and forming suspension, the first part is used for accommodating the suspension, and the third part is used for being connected with the external environment so as to discharge the excessive eluent.

Further, the central axis of the current carrying pipeline is defined to be in a first direction, the drainage surface comprises a first drainage surface and a second drainage surface connected with the first drainage surface, the first drainage surface is obliquely arranged with the first direction, the second drainage surface is parallel to the first direction, one end of the first drainage surface, deviating from the second drainage surface, is connected with the inner wall of the current carrying pipe, and one end of the second drainage surface, deviating from the first drainage surface, is connected with the first part.

Further, the first and second flow-discharging surfaces are both plane surfaces, and the included angle between the first flow-discharging surface and the first direction is 15-45 degrees.

Further, the elution chamber includes first lateral wall, first diapire, second lateral wall and second diapire, first lateral wall connects first diapire is in order to enclose to establish and is formed first part, the second lateral wall is connected the second diapire is in order to form the third part, first lateral wall with the relative interval of second lateral wall sets up, first diapire is connected the second diapire, first lateral wall deviates from the one end of first diapire and the one end that the second lateral wall deviates from the second diapire is connected the current-carrying pipeline.

Further, the elution chamber further includes a protrusion, the protrusion is disposed at an end of the second side wall facing away from the second bottom wall, the protrusion extends toward the first side wall, a side of the protrusion facing away from the second bottom wall is connected with the leakage fluid, the protrusion faces toward a side concave shape Cheng Kacao of the first portion, and the second portion includes the clamping groove.

Further, the elution chamber further comprises a liquid discharge pipe, an opening is formed in the second side wall in a penetrating mode, and the liquid discharge pipe is communicated with the opening.

Further, the thickness of the second bottom wall is greater than the thickness of the first bottom wall such that the third portion is offset from the first portion.

Further, the jet piece comprises a liquid storage device, a driver and a jet nozzle, wherein the liquid storage device is used for storing the eluent, the driver is communicated with the liquid storage device and the jet nozzle so as to drive the eluent in the liquid storage device to flow to the jet nozzle, the jet nozzle comprises a first pipeline and a second pipeline communicated with the first pipeline, the first pipeline penetrates through the current carrying pipe, the second pipeline is communicated with the first pipeline extending into the current carrying pipe, and a liquid outlet port of the second pipeline is arranged towards a leakage surface.

Further, the jet piece comprises a liquid inlet pipe, a liquid outlet pipe and a driver arranged between the liquid inlet pipe and the liquid outlet pipe, wherein the liquid inlet pipe stretches into the bottom of the first part, the liquid outlet pipe stretches into the side part of the first part, the liquid outlet pipe is used for being arranged towards the filter membrane, and the driver is used for driving the suspension liquid of the first part to be ejected towards the filter membrane.

A separation device for separating a target in a liquid sample and obtaining a suspension of the target, comprising:

an elution structure as described above;

a liquid feeder communicated with the first part of the elution structure for injecting a liquid sample into the first part;

a filter membrane disposed in a second portion of the elution structure for filtering the liquid sample and depositing the target on one side of the filter membrane;

a vacuum generator in communication with a third portion of the elution structure for creating a negative pressure in the third portion for forcing the liquid sample in the first portion to flow toward the filter membrane;

and the controller is electrically connected with the liquid inlet device and the vacuum generator and is used for controlling the flow of the liquid sample and the magnitude of the negative pressure.

Compared with the prior art, the elution structure provided by the application is characterized in that the carrier piece is internally provided with the drainage fluid with the drainage surface, and the eluent emitted by the jet piece can flow to the top end part of the filter membrane on the basis of the wall attaching effect and is then sprayed from the top downwards to deposit the target object on one side of the filter membrane and form suspension. The inclined flow leakage surface can reduce the splashing of the eluent caused by the too high flow velocity, thereby reducing the damage to the target object. Meanwhile, the jet flow width of the eluent can be effectively increased by the flow leakage surface, so that the eluting efficiency of the eluent is improved.

Drawings

Fig. 1 is an overall schematic diagram of an elution structure provided in a first embodiment of the present application.

FIG. 2 is a cross-sectional view of the elution structure shown in FIG. 1 taken along line II-II.

Fig. 3 is a cross-sectional view of an elution structure provided in a second embodiment of the present application.

Fig. 4 is a cross-sectional view of an elution structure provided in a third embodiment of the present application.

Fig. 5 is a connection block diagram of a separation device according to a first embodiment of the present application.

Description of the main reference signs

Elution arrangements 100, 100'

Jet member 10, 10'

Jet nozzle 11

First pipe 111

Second pipe 112

Liquid inlet pipe 12

Liquid outlet pipe 13

Current carrying member 20

Carrying conduit 21

Side plate 211

Venting body 22

Relief surface 221

First relief surface 221a

Second relief surface 221b

Bearing surface 222

Elution chamber 30

First portion 31

First side wall 31a

First bottom wall 31b

Second portion 32

Second side wall 33a

A second bottom wall 33b

Opening 33c

Drain pipe 33d

Third portion 33

Convex portion 34

Card slot 341

Liquid feeder 40

Vacuum generator 50

Controller 60

Filter membrane 200

Target 201

Eluent 202

Suspension 203

Separation device 400

Included angle alpha

First direction A

Second direction B

Thickness H1, H2

The following detailed description will further illustrate the application in conjunction with the above-described figures.

Detailed Description

The technical solution of the present application will be described below in connection with preferred embodiments and examples of the present application. It will be noted that when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. When an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The names of elements or devices used in the specification of the present application are for the purpose of describing specific embodiments only, and are not intended to limit the present application.

Referring to fig. 1 and 2, a first embodiment of the present application provides an elution structure 100, and the elution structure 100 can be used to elute a target 201 deposited on one side of a filter membrane 200. The filter 200 may be a filter with a pore size of nanometer scale, and the target 201 may be a membranous vesicle.

Referring to fig. 2, elution structure 100 includes fluidic component 10, current carrying component 20, and elution chamber 30. The jet member 10 is configured to jet the eluent 202, which may be a phosphate buffer or a physiological saline, toward the current carrying member 20. Carrier 20 is used to direct eluent 202 into elution chamber 30. Elution chamber 30 is used to provide a filter 200 on one side of which target 201 is deposited, and eluent 202 is used to elute the target 201 and form suspension 203. Suspension 203 may be contained within elution chamber 30. Wherein the eluent 202 may pass through the micropores of the filter membrane 200, while the target 201 cannot pass through the micropores of the filter membrane 200 due to its large size.

The current carrying member 20 includes a current carrying pipe 21 and a leakage fluid 22 disposed in the current carrying pipe 21. The drainage body 22 has a drainage surface 221 disposed obliquely, one end of the drainage surface 221 is connected to the inner wall of the current carrying pipe 21, and the drainage surface 221 is used for guiding the eluent 202 to flow. Wherein, defining the central axis of the current carrying pipe 21 as the first direction a (i.e., the eluent 202 is defined in the current carrying pipe 21 to flow along the first direction a), the inclined drainage surface 221 includes a drainage surface 221 inclined to the first direction a.

Elution chamber 30 is connected to current carrier 20, elution chamber 30 comprising, in order from right to left, a first portion 31, a second portion 32, and a third portion 33. The second portion 32 communicates between the first portion 31 and the third portion 33. Wherein the second portion 32 is used for disposing the filter membrane 200 for depositing the target 201, and one side of the filter membrane 200 on which the target 201 is deposited is directed toward the first portion 31 and the other opposite side of the filter membrane 200 on which the target 201 is not deposited is directed toward the third portion 33. The end of the first portion 31 facing the second portion 32 is connected to the drainage surface 221, so that the eluent 202 can flow from the drainage surface 221 to the top end of the filter membrane 200, and part of the eluent 202 can elute the deposited target 201 and form the suspension 203 and be contained in the first portion 31. Another portion of the eluent 202 may pass through the micropores of the filter membrane 200 into the third portion 33, and the third portion 33 communicates with the external environment, thereby draining the remaining eluent 202.

Compared to the prior art, the elution structure 100 provided in the present application provides that by disposing the drain fluid 22 having the drain surface 221 in the current carrying member 20, the eluent 202 emitted from the jet member 10 can flow to the top end portion of the filter membrane 200 depending on the drain surface 221 based on coanda Effect (coanda Effect), and then the eluent 202 is eluted from the top down to the target 201 deposited on one side of the filter membrane 200 and forms the suspension 203. The inclined flow-off surface 221 reduces splashing of the eluent 202 due to too high a flow rate, thereby reducing damage to the target 201. Meanwhile, the drainage surface 221 can effectively increase the jet width of the eluent 202, thereby improving the elution efficiency of the eluent 202.

Referring to fig. 1 and 2, in the present embodiment, the current carrying pipe 21 is substantially square, the current carrying pipe 21 includes a plurality of end-to-end side plates 211, the drain fluid 22 is substantially wedge-shaped, and the drain fluid 22 and the current carrying pipe 21 are integrally formed. The leakage body 22 further includes a receiving surface 222 connected to the side plate 211, and the receiving surface 222 is flush with an end surface of the leakage body 22. The receiving surface 222 is connected between the inner wall of the current carrying pipe 21 and the relief surface 221. The relief surface 221 includes a first relief surface 221a disposed obliquely to the first direction a and a second relief surface 221b disposed parallel to the first direction a, and the first relief surface 221a connects the second relief surface 221b. One end of the first drainage surface 221a facing away from the second drainage surface 221b is connected to the inner wall of the current carrying pipe 21, and one end of the second drainage surface 221b facing away from the first drainage surface 221a is connected to one end of the first portion 31. The eluent 202 may have a first drainage surface 221a flowing to a second drainage surface 221b, and then flow from the second drainage surface 221b to the filter membrane 200, so that the filter membrane 200 may be disposed in the second portion 32 substantially along a direction parallel to the first direction a by disposing the second drainage surface 221b parallel to the first direction a, that is, the flowing direction of the eluent 202 on the second drainage surface 221b is substantially parallel to the disposing direction of the filter membrane 200, thereby being beneficial to further improving the eluting efficiency.

Referring to fig. 1 and 2, in the present embodiment, the first drainage surface 221a and the second drainage surface 221b are both flat, and the eluent 202 flows from the slope to the filter membrane 200 by the wall attachment of the liquid. Therefore, the angle between the first drainage surface 221a and the first direction a cannot be too large, otherwise, the eluent 202 is relatively easy to be separated (splashed) from the first drainage surface 221a or the second drainage surface 221b directly without flowing to the filter membrane 200, and if the angle between the first drainage surface 221a and the first direction a is too large, the flow rate of the flushing of the eluent 202 needs to be reduced, so that the eluting effect is greatly reduced. Meanwhile, the included angle between the first drainage surface 221a and the first direction a cannot be too small, otherwise, the length of the first drainage surface 221a is relatively long, which is not beneficial to miniaturizing the elution structure 100. Therefore, the angle α between the first drainage surface 221a and the first direction a is 15 ° to 45 °. Preferably, the first relief surface 221a is at an angle α of 20 ° to the first direction a. It can be appreciated that in other embodiments of the present application, the first drainage surface 221a and the second drainage surface 221b may be cambered surfaces, or may be any other irregular smooth curved surfaces, so as to better guide the eluent to flow through, and also reduce the requirement on the ejection accuracy of the hedging jet member 10. In addition, the first drainage surface 221a and/or the second drainage surface 221b may be subjected to surface treatment to change the hydrophilicity or hydrophobicity of the first drainage surface 221a and/or the second drainage surface 221b, so as to adjust the flow rate of the eluent 202 on the first drainage surface 221a and the second drainage surface 221b, so that the flow rate of the eluent 202 is more suitable for the elution target 201. Preferably, the first drainage surface 221a and/or the second drainage surface 221b is subjected to surface hydrophilic treatment.

Referring to fig. 1 and 2, in the present embodiment, the elution chamber 30 includes a first side wall 31a, a first bottom wall 31b, a second side wall 33a, a second bottom wall 33b, and a protrusion 34. The first side wall 31a connects the first bottom wall 31b to form the first portion 31. The second side wall 33a connects the second bottom wall 33b to form the third portion 33. The first side wall 31a is disposed corresponding to the second side wall 33a, and the first side wall 31a and the second side wall 33a are disposed substantially parallel to the first direction a, one end of the first side wall 31a is connected to the side plate 211, and one end of the second side wall 33a is connected to the other side plate 211. Defining a second direction B perpendicular to the first direction a, the first bottom wall 31B is connected to the second bottom wall 33B substantially along the second direction B, and the first bottom wall 31B and the second bottom wall 33B are disposed between the first side wall 31a and the second side wall 33 a. The protruding portion 34 is disposed at one end of the second sidewall 33a and extends toward the third portion 33, and the protruding portion 34 is used for supporting the leakage fluid 22. The protruding portion 34 is disposed corresponding to the second bottom wall 33b, and the protruding portion 34 is recessed Cheng Kacao toward one side of the first portion 31, and the clamping groove 341 forms the second portion 32 along the first direction a corresponding to the portions of the first portion 31 and the third portion 33. Wherein, the bottom of the second side wall 33a is provided with an opening 33c, a part of the second side wall 33a extends around the opening 33c along the first direction a to form a liquid discharge tube 33d, and the eluent 202 can flow into the liquid discharge tube 33d through the opening 33c and be discharged to the external environment from the liquid discharge tube 33 d. In the present embodiment, the first side wall 31a, the first bottom wall 31b, the second side wall 33a, the second bottom wall 33b, and the convex portion 34 are integrally formed.

Referring to fig. 1 and 2, in the present embodiment, the thickness H1 of the second bottom wall 33b is greater than the thickness H2 of the first bottom wall 31b, so that the end face of the second side wall 33a can be used to abut against the filter membrane 200, and at the same time, the thickness H1 of the second bottom wall 33b is greater than the thickness H2 of the first bottom wall 31b as viewed along the first direction a, so that the third portion 33 is staggered from the first portion 31, thereby facilitating the first portion 31 to store a small amount of the suspension 203, so that the suspension 203 does not flow into the third portion 33 entirely and is discharged to the external environment.

Referring to fig. 1 and 2, in the present embodiment, the fluidic component 10 is separable from the current carrying component 20 or the elution chamber 30, and the fluidic component 10 includes a reservoir (not shown), a driver (not shown), and a fluidic nozzle 11. The liquid storage device is used for storing a proper amount of eluent 202, the driver is communicated with the liquid storage device and the jet nozzle 11, the driver is used for driving the eluent 202 in the liquid storage device to flow to the jet nozzle 11, and the jet nozzle 11 is used for directionally ejecting the eluent 202. In particular use, the jet nozzle 11 protrudes into the carrier conduit 21 and is directed towards a first drainage surface 221a arranged obliquely to the first direction a for ejecting the eluent 202. After the elution of the target 201 is completed, the jet nozzle 11 can be pulled out of the current carrying pipe 21. Specifically, the driver is a peristaltic pump. It will be appreciated that in other embodiments of the present application, the fluidic 10 may be a syringe.

Referring to fig. 3, a second embodiment of the present application provides an elution structure 100', which is different from the elution structure 100 of the first embodiment in that: the jet nozzle 11 of the jet member 10' is fixedly arranged in the current carrying conduit 21. Specifically, the jet nozzle 11 has a substantially "L" shape, and the jet nozzle 11 includes a first pipe 111 and a second pipe 112 that vertically communicates with the first pipe 111. The first pipe 111 passes through the side plate 211 of the carrying pipe 21, the second pipe 112 communicates with the first pipe 111 extending into the carrying pipe 21, and the liquid outlet port of the second pipe 112 is disposed toward the first drain surface 221 a. That is, the second conduit 112 is positioned relative to the first drain surface 221a, thereby ensuring that the nozzle 11 continuously or intermittently emits the eluent 202 toward the first drain surface 221a in a constant direction, which is advantageous in reducing splashing of the eluent 202.

Referring to fig. 4, a third embodiment of the present application provides an elution structure 100″ different from the elution structure 100 of the first embodiment in that: fluidic 10 "is fixedly disposed in elution chamber 30 and fluidic 10 can repeatedly aspirate and eject suspension 203. Specifically, the fluidic element 10 includes a liquid inlet tube 12, a liquid outlet tube 13, and a driver 14 disposed between the liquid inlet tube 12 and the liquid outlet tube 13. One end of the liquid inlet pipe 12 passes through the first bottom wall 31b and is inserted into the first portion 31 to suck the suspension 203. The outlet pipe 13 passes through the first side wall 31a and is inserted into the first portion 31, and the outlet port of the outlet pipe 13 is directed towards the filter membrane 200. The driver 14 may continuously or intermittently extract the suspension 203 from the bottom of the first portion 31 to one side of the bottom of the filter membrane 200, thereby creating an elution cycle and thereby enhancing elution of the target 201 at the bottom of the filter membrane 200. It will be appreciated that in other embodiments of the present application, elution structure 300 may further include an additional fluidic component 10 disposed on current carrying component 20, and that additional fluidic component 10 may be configured to spray elution fluid 202 toward drainage surface 221, thereby rapidly eluting one side target 201 on top of filter membrane 200. That is, by the cooperation of the jet member 10 provided in the elution chamber 30 and the jet member 10 provided in the current carrying member 20, the elution efficiency of the target 201 at the bottom of the filter membrane 200 and at the top of the filter membrane 200 is improved, respectively. Specifically, the driver 14 may be a motor driven pump or a manually driven rubber hollow ball.

Referring to fig. 1, fig. 2, and fig. 5, the first embodiment of the present application further provides a separation device 400, where the separation device 400 is used for performing multi-stage separation and purification on particles with different sizes in a liquid sample, so as to obtain a suspension 203 of a plurality of target objects 201 with specific sizes. The liquid sample can be human plasma, serum, cerebral spinal fluid, saliva, urine, tears, etc. The separation device 400 includes the elution structure 100, the filter membrane 200 disposed on the elution structure 100, the liquid feeder 40 and the vacuum generator 50 in communication with the elution structure 100, and the controller 60 electrically connected to the liquid feeder 40 and the vacuum generator 50. Wherein the liquid feeder 40 is used for injecting a liquid sample (not shown) into the current carrier member 20. The vacuum generator 50 is used to create a negative pressure in the elution chamber 30 such that the liquid sample flows into the filter membrane 200 to be filtered, and the target 201 is trapped on one side of the filter membrane 200. The controller 60 is used for controlling the vacuum generator 50 and the liquid feeder 40 to adjust the magnitude of the negative pressure in the elution chamber 30 and the injection amount of the liquid sample. Wherein the separation device 400 can be used to separate the target 201 from the liquid sample and then elute the target 201 from the filter membrane 200.

Referring to fig. 1, 2 and 5, in the present embodiment, the liquid feeder 40 is connected to the first portion 31, the filter membrane 200 is disposed on the second portion 32 of the elution structure 100, and the vacuum generator 50 is connected to the third portion 33. Specifically, the liquid feeder 40 is an aspiration type liquid feeder or a push type liquid feeder. The filter membrane 200 is any one of an anodic aluminum oxide membrane, an acetate fiber membrane, a polyethylene membrane, a polypropylene membrane, or a polystyrene membrane. The vacuum generator 50 may be any one of a vacuum pump, a diaphragm pump, a peristaltic pump, a syringe, and a spout-type vacuum generator. The controller 60 is a programmable logic controller.

Referring to fig. 1, fig. 2, and fig. 5, the first embodiment of the present application further provides a method for using the separation device 400, which specifically includes the steps of:

the separation device 400 and the liquid sample are provided S1, the liquid sample is injected into the current carrying pipe 21 of the separation device 400 through the liquid feeder 40, and the liquid sample flows into the first portion 31 of the elution chamber 30.

The vacuum generator 50 draws from the third portion 33 of the elution chamber 30 to create a negative pressure in the third portion 33 and the liquid sample flows through the filter membrane 200 to the third portion 33. At the same time, the target 201 in the liquid sample is trapped by the filter membrane 200 and deposited on the side of the filter membrane 200 facing the first portion 31 until the liquid sample is completely filtered, and the vacuum generator 50 stops the suction.

S3, the jet member 10 emits the eluent 202 towards the drainage surface 221, and the eluent 202 elutes the target 201 deposited on one side of the filter membrane 200 from top to bottom to form the suspension 203 containing the target 201.

The above description is only one preferred embodiment of the present utility model, but is not limited to this embodiment during actual application. Other modifications and variations to the present utility model which are within the scope of the claims of the present utility model will be apparent to those of ordinary skill in the art.

Claims (10)

1. An elution structure for eluting a target deposited on one side of a filter membrane, the elution structure comprising:

the jet piece is used for jetting the eluent;

the current carrying piece comprises a current carrying pipeline and a leakage fluid arranged in the current carrying pipeline, wherein the leakage fluid comprises a leakage surface which is obliquely arranged, and one end of the leakage surface is connected with the inner wall of the current carrying pipeline;

the elution cavity is connected with the current carrying piece and comprises a first part, a second part and a third part which are communicated with the current carrying pipeline, the second part is communicated between the first part and the third part, the second part is used for arranging the filter membrane, the other end of the drainage surface is used for being connected with one end of the filter membrane so as to guide the eluent to the top end of the filter membrane, the eluent is used for eluting the target object deposited on the filter membrane and forming suspension, the first part is used for accommodating the suspension, and the third part is used for being connected with the external environment so as to discharge the excessive eluent.

2. The eluting structure according to claim 1, wherein a central axis of the current carrying pipe is defined as a first direction, the drainage surface includes a first drainage surface and a second drainage surface connected to the first drainage surface, the first drainage surface is obliquely arranged with respect to the first direction, the second drainage surface is parallel to the first direction, an end of the first drainage surface facing away from the second drainage surface is connected to an inner wall of the current carrying pipe, and an end of the second drainage surface facing away from the first drainage surface is connected to the first portion.

3. The elution structure of claim 2 wherein said first and second venting surfaces are planar and said first venting surface is angled from 15 ° to 45 ° from said first direction.

4. The elution structure of claim 1 wherein the elution chamber comprises a first side wall, a first bottom wall, a second side wall, and a second bottom wall, the first side wall connecting the first bottom wall to enclose to form the first portion, the second side wall connecting the second bottom wall to form the third portion, the first side wall and the second side wall being disposed in opposing spaced relation, the first bottom wall connecting the second bottom wall, an end of the first side wall facing away from the first bottom wall, and an end of the second side wall facing away from the second bottom wall both connecting the current carrying conduit.

5. The elution structure of claim 4 wherein said elution chamber further comprises a protrusion disposed at an end of said second side wall facing away from said second bottom wall, said protrusion extending toward a first side wall, a side of said protrusion facing away from said second bottom wall connecting said drain fluid, a side of said protrusion facing toward said first portion being concave Cheng Kacao, said second portion comprising said catch.

6. The elution structure of claim 4 wherein said elution chamber further comprises a drain, said second sidewall being provided with an opening therethrough, said drain being in communication with said opening.

7. The elution structure of claim 4 wherein the thickness of the second bottom wall is greater than the thickness of the first bottom wall such that the third portion is offset from the first portion.

8. The eluting structure according to claim 1, wherein the fluidic member includes a reservoir for storing the eluent, a driver in communication with the reservoir and the fluidic nozzle for driving the eluent in the reservoir to flow toward the fluidic nozzle, and a fluidic nozzle including a first conduit and a second conduit in communication with the first conduit, the first conduit passing through the carrier conduit, the second conduit in communication with the first conduit extending into the carrier conduit, the outlet port of the second conduit being disposed toward the drain surface.

9. The elution structure of claim 1 wherein the fluidic member comprises a liquid inlet tube, a liquid outlet tube, and a driver disposed between the liquid inlet tube and the liquid outlet tube, the liquid inlet tube extending into the bottom of the first portion, the liquid outlet tube extending into the side of the first portion, the liquid outlet tube being configured to be disposed toward the filter membrane, the driver being configured to drive the suspension of the first portion to be ejected toward the filter membrane.

10. A separation device for separating a target in a liquid sample and obtaining a suspension of the target, the separation device comprising:

an elution structure as claimed in any one of claims 1 to 9;

a liquid feeder communicated with the first part of the elution structure for injecting a liquid sample into the first part;

a filter membrane disposed in a second portion of the elution structure for filtering the liquid sample and depositing the target on one side of the filter membrane;

a vacuum generator in communication with a third portion of the elution structure for creating a negative pressure in the third portion for forcing the liquid sample in the first portion to flow toward the filter membrane;

and the controller is electrically connected with the liquid inlet device and the vacuum generator and is used for controlling the flow of the liquid sample and the magnitude of the negative pressure.

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