CN219207455U

CN219207455U - Mouse eyeball picking and hemostix

Info

Publication number: CN219207455U
Application number: CN202222968697.4U
Authority: CN
Inventors: 楼晓昆; 公文艳; 郭俊锋; 丁盈盈; 高丽娜
Original assignee: Hangzhou Normal University
Current assignee: Hangzhou Normal University
Priority date: 2022-11-08
Filing date: 2022-11-08
Publication date: 2023-06-20
Anticipated expiration: 2032-11-08

Abstract

The utility model relates to a mouse eyeball picking and hemostix. The utility model comprises a blood taking needle, an anti-drop device, a negative pressure cavity and an eye ball clamp; one end of the anti-drop device is fixed with the middle part of the blood taking needle, and the other end of the anti-drop device and one end of the negative pressure cavity are integrally formed by arranging steps; the piston is arranged outside the anti-drop device and the blood taking needle in the negative pressure cavity, one end of the blood taking needle passes through the anti-drop device, and the other end of the blood taking needle passes through the piston head and the piston shaft and is flush with the other end of the negative pressure cavity; the piston shaft is provided with a piston force application point, and a sliding rail is arranged at a corresponding position on the anti-drop device; the eye ball forceps are arranged outside the anti-drop device; the lower end of the negative pressure cavity is provided with a microcentrifuge tube fixing clip. The utility model is suitable for the scene of taking eyeball blood from mice, and is operated by a single instrument, so that the experimental sampling efficiency is improved, the blood taking sample quantity is ensured, and the pollution of blood samples is reduced.

Description

Mouse eyeball picking and hemostix

Technical Field

The patent relates to the field of medical animal experiment appliances, and relates to a mouse eyeball picking and hemostix.

Background

Experimental studies in mice play an important role in modern medical biology research. Blood is one of the important components reflecting the condition of the body of an animal. In the mouse experiment, the mice were small in size and only blood was collected through the eyes to obtain a large amount of clean blood. There are two traditional ocular blood sampling methods, one of which is blood sampling without picking the eyeball, namely blood sampling of the retroorbital venous plexus: the left thumb and the index finger grip the neck of the mouse, press the two sides of the neck to bleed the orbital venous plexus, and the right hand capillary tube pierces 2-3mm from the inner canthus at an angle of 45 degrees, so that the method is commonly used for continuous multiple blood sampling of the mouse; secondly, collecting blood by taking the eyeball: the mouse is held by one hand, the forceps are held by the other hand, a microcentrifuge tube which needs to be connected with blood is placed under the forceps, the root of the eyeball of the mouse is clamped by the forceps, the eyeball of the mouse is separated, and blood is discharged from a blood vessel which supplies the eyeball, but the mouse is easy to blindness, infection or death caused by inaccurate hemostasis, and is usually used for end-stage sampling of experiments.

When sampling, especially when taking out the eyeball and taking the blood, the blood can not drop into the microcentrifuge tube due to the fact that the mice shake due to pain; meanwhile, in the process of sample transfer, the loss and pollution of mouse blood are easy to cause.

Based on the above, the mouse eyeball picking and hemostix is designed, and can be used for rapidly collecting blood after an experimenter picks the mouse eyeball, reducing the transfer times of samples, improving the collection efficiency of the blood samples of the mice, guaranteeing the sample quantity of the blood and reducing the sample pollution.

Disclosure of Invention

The utility model aims to provide a mouse eyeball picking and hemostix, which is suitable for a scene of picking eyeballs to pick blood from a mouse, is operated by a single instrument, improves the experimental sampling efficiency, ensures the blood-picking sample quantity and reduces the pollution of blood samples.

The utility model comprises a blood taking needle, an anti-drop device, a negative pressure cavity and an eye ball clamp; the anti-drop device and the negative pressure cavity are hollow cylinder structures, and the cross section diameter of the anti-drop device is smaller than that of the negative pressure cavity; one end of the anti-drop device is fixed with the middle part of the blood taking needle, and the other end of the anti-drop device and one end of the negative pressure cavity are integrally formed by arranging steps;

the piston comprises a piston head and a piston shaft, and blood taking needle mounting holes are formed in the middle parts of the piston head and the piston shaft; one end of the blood taking needle passes through the anti-drop device, and the other end of the blood taking needle passes through the piston head and the piston shaft and is flush with the other end of the negative pressure cavity;

the piston head is arranged in the negative pressure cavity and moves along the negative pressure cavity under the drive of the piston shaft; one end of the piston shaft is fixed with the piston head, the other end of the piston shaft is provided with a piston force point, and a sliding rail is arranged at a corresponding position on the anti-drop device;

the eye ball forceps are arranged outside the anti-drop device, the eye ball forceps are in a forceps shape, the fixed ends of the two forceps feet are provided with eye ball forceps fixing platforms, the eye forceps fixing platforms are provided with eye forceps fixing holes, and the eye forceps fixing holes are in interference fit with the anti-drop device; a step protruding to the periphery is arranged at the fixed end of the blood taking needle of the anti-drop device; the eyeball forceps fixing platform is also provided with a piston force point penetrating hole;

the lower end of the negative pressure cavity is provided with a microcentrifuge tube fixing clamp which is of a microcentrifuge tube cap structure with two ends open and is directly embedded with the orifice of the microcentrifuge tube.

One end of the anti-drop device is fixed with the middle part of the blood taking needle through the opening and the sealing ring.

The piston force point is a columnar mounting strip, one end of the piston force point is integrally formed with the piston shaft, and the other end of the piston force point penetrates through the sliding rail.

The piston head adopts a thickened butyl rubber film.

The middle upper part of the eye ball forceps is provided with friction lines.

When the utility model is used for taking blood, the hand can adjust the position of the needle tube, thereby effectively preventing insufficient blood collection caused by shaking of the head of a mouse and increasing the blood collection amount; the anastomotic site of the micro centrifuge tube at the lower end of the negative pressure cavity can be matched and anastomosed with the micro centrifuge tube, so that the device is integrated, and the operation equipment is simple; the finger can adjust the time of negative pressure, and can freely adjust the suction according to the bleeding amount of the eyeballs of the mice, so as to realize controllable liquid taking; blood enters the micro centrifuge tube directly after entering the needle tube, and blood coagulation, loss and pollution are reduced. Can be operated by a single person, and improves the experimental efficiency.

Drawings

FIG. 1 is a schematic diagram of the overall structure of the present utility model;

FIG. 2 is a schematic view of the assembly of the fixation platform of the eye clamp of FIG. 1;

fig. 3 is a use state diagram of the present utility model.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

As shown in fig. 1, a mouse eyeball extraction and hemostix comprises a blood taking needle 1, an anti-drop device 6, a negative pressure cavity 7 and an eyeball forceps 9.

The anti-drop device 6 and the negative pressure cavity 7 are hollow cylinder structures, and the diameter of the section of the anti-drop device 6 is smaller than that of the section of the negative pressure cavity 7; one end of the anti-drop device 6 is used as a blood taking needle fixing end, and the blood taking needle 1 is fixed through the opening and the sealing ring; the other end of the anti-drop device 6 and one end of the negative pressure cavity 7 are integrally formed by arranging steps; the blood taking needle 1 is hollow, one end (working end) of the blood taking needle 1 passes through the anti-drop device 6, and the middle part is fixed with the anti-drop device 6.

The outside of the blood taking needle 1 in the anti-drop device 6 and the negative pressure cavity 7 is provided with a piston, the piston comprises a piston head 2 and a piston shaft 3, blood taking needle mounting holes are formed in the middle parts of the piston head 2 and the piston shaft 3, and the other end of the blood taking needle 1 penetrates through the piston head 2 and the piston shaft 3 and is flush with the other end of the negative pressure cavity 7.

The piston head 2 is arranged in the negative pressure cavity 7 and moves along the negative pressure cavity 7 under the drive of the piston shaft 3; one end of the piston shaft 3 is fixed with the piston head 2, a pair of piston force points 4 are symmetrically and fixedly arranged at the other end of the piston shaft 3, a pair of sliding rails 5 are arranged at corresponding positions on the anti-drop device 6, the piston force points 4 are columnar mounting strips, one end of each piston force point 4 and the piston shaft 3 are integrally formed, and the other end of each piston force point 4 penetrates through each sliding rail 5, so that the piston shaft 3 is driven by the corresponding piston force point 4 to move along the corresponding sliding rail 5. The length of the piston shaft 3 is smaller than that of the anti-drop device 6.

The piston head 2 adopts a thickened butyl rubber film, the piston head 2 is in transitional fit with the blood taking needle, air leakage can be prevented in the sliding process, negative pressure formation failure is avoided, and blood taking failure is further caused.

The eye ball forceps 9 are arranged outside the anti-drop device 6, the eye ball forceps 9 are in a forceps shape, and friction lines 10 are arranged at the middle upper part of the eye ball forceps 9. The fixed ends of the two forceps feet are provided with an eye forceps fixing platform, as shown in fig. 2, an eye forceps fixing hole 12 is formed in the eye forceps fixing platform, the eye forceps fixing hole 12 is in interference fit with the anti-drop device 6, so that the eye forceps fixing hole 12 is nested outside the anti-drop device 6 and can move along the anti-drop device 6, and the fixed end of a blood taking needle of the anti-drop device 6 is provided with a step protruding to the periphery and used for limiting the anti-drop device 6; meanwhile, in order not to influence the movement of the piston shaft 3, a piston force point penetrating hole 13 is further formed in the eyeball pincers fixing platform, and when blood is taken, the piston force point 4 penetrates through the piston force point penetrating hole 13 to drive the piston to move, so that blood collection is completed. The length of the eyeball pincers 9 is smaller than the length of the part, which is not in the negative pressure cavity, of the blood taking needle, and when the fixed ends of the two forceps feet of the eyeball pincers 9 are positioned at the lowest end of the anti-drop device 6, the working ends of the two forceps feet of the eyeball pincers 9 are lower than the working end of the blood taking needle.

The lower end of the negative pressure cavity 7 is provided with a microcentrifuge tube fixing clamp 8, and the microcentrifuge tube fixing clamp 8 is of a microcentrifuge tube cap structure with two open ends and can be directly embedded into a tube orifice of a microcentrifuge tube. One end of the microcentrifuge tube fixing clamp 8 is connected with the negative pressure cavity 7, and the other end is connected with the microcentrifuge tube, so that the collected blood sample is directly input into the microcentrifuge tube 11.

When the device is used, the device is in a natural state, the eyeball pincers 9 are in the lowest position, the piston head is in the lowest position of the negative pressure cavity, and the piston force application point 4 is in the lowest position of the sliding rail 5.

Before use, after some anticoagulants such as heparin are sucked to moisten the blood taking needle, the microcentrifuge tube is fixed on the microcentrifuge tube fixing card 8.

When the eye ball forceps are used, the thumb and the index finger press the friction lines 10 of the eye ball forceps 9, the eye ball forceps 9 are pushed upwards to exceed the needle heads, eyeballs are clamped and picked, and then the eye ball forceps 9 are loosened, and the eye ball forceps fall back due to gravity; when the positions of the piston force point penetrating holes 13 and the piston force point 4 are not aligned, the positions are slightly adjusted by using the thumb and the index finger; after falling back, the blood taking needle is exposed, the blood taking needle head is placed at a bleeding point at 45 degrees, the thumb applies force upwards to the piston force point 4 to drive the piston shaft 3 to move upwards along the sliding rail 5, so that the piston head 2 moves upwards in negative pressure to suck blood into the microcentrifuge tube. The above operation ensures the full collection of blood and avoids pollution in the blood taking process.

It is noted that relational terms such as used and not used, and the like, are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present utility model. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the utility model. Thus, the present utility model is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A mouse eyeball is taken and hemostix, its characterized in that: comprises a blood taking needle, an anti-drop device, a negative pressure cavity and an eye ball clamp; the anti-drop device and the negative pressure cavity are hollow cylinder structures, and the cross section diameter of the anti-drop device is smaller than that of the negative pressure cavity; one end of the anti-drop device is fixed with the middle part of the blood taking needle, and the other end of the anti-drop device and one end of the negative pressure cavity are integrally formed by arranging steps;

2. The mouse eyeball extraction and hemostix of claim 1 wherein: one end of the anti-drop device is fixed with the middle part of the blood taking needle through the opening and the sealing ring.

3. The mouse eyeball extraction and hemostix of claim 1 wherein: the piston force point is a columnar mounting strip, one end of the piston force point is integrally formed with the piston shaft, and the other end of the piston force point penetrates through the sliding rail.

4. The mouse eyeball extraction and hemostix of claim 1 wherein: the piston head adopts a thickened butyl rubber film.

5. The mouse eyeball extraction and hemostix of claim 1 wherein: the middle upper part of the eye ball forceps is provided with friction lines.