CN219174514U

CN219174514U - Shaking device for cell preparation

Info

Publication number: CN219174514U
Application number: CN202320353140.1U
Authority: CN
Inventors: 刘斌; 师建国; 张嘉颖; 霍剑妮
Original assignee: Xi'an Meimei Hongkang Biotechnology Co ltd
Current assignee: Xi'an Meimei Hongkang Biotechnology Co ltd
Priority date: 2023-03-01
Filing date: 2023-03-01
Publication date: 2023-06-13
Anticipated expiration: 2033-03-01

Abstract

The utility model belongs to the technical field of cell preparation, and particularly relates to a shaking device for cell preparation, which comprises a supporting component and a shaking mechanism, wherein the shaking mechanism is arranged on the inner side of the supporting component and is obliquely arranged; the shaking mechanism comprises a shaking tank, the shaking mechanism is arranged on the inner side of the supporting component, an inner container is arranged in the shaking tank, the shaking tank is rotationally connected with the inner container, a cell shaking chamber for shaking cells is arranged in the middle of the inner container, a test tube fixing hole is further formed in the inner container, a motor is arranged at the bottom of the shaking tank, and the output end of the motor is connected with the inner container; the bottom of the shaking tank is provided with a storage battery. The shaking tank is obliquely arranged, and can be rotated when stirring the cell liquid, so that the cell liquid moves irregularly during stirring, the shaking effect is better, the shaking time can be reduced, and the preparation speed is higher.

Description

Shaking device for cell preparation

Technical Field

The utility model belongs to the technical field of cell preparation, and particularly relates to a shaking device for cell preparation.

Background

Cell preparation methods generally adopt cell suspension culture for non-anchorage dependent cells, wherein the suspension culture refers to a tissue culture system for culturing single cells and small cell clusters in a liquid culture medium which is continuously stirred or rocked, and the cell suspension culture is a culture mode for the non-anchorage dependent cells and needs to shake cell fluid when the preparation is carried out;

the shaking equipment adopted at present can only vertically rotate when shaking cell sap, the shaking effect is poor, and the preparation can be completed after shaking for a longer time.

Disclosure of Invention

The utility model aims to provide a shaking device for preparing cells, which is characterized in that a shaking tank of the device is obliquely arranged, and the shaking tank can be rotated when stirring cell liquid, so that the cell liquid moves irregularly during stirring, the shaking effect is better, the shaking time can be reduced, and the preparation speed is higher.

The technical scheme adopted by the utility model is as follows:

the shaking device for preparing the cells comprises a supporting component and a shaking mechanism, wherein the shaking mechanism is arranged on the inner side of the supporting component and is obliquely arranged;

the shaking mechanism comprises a shaking tank, the shaking mechanism is arranged on the inner side of the supporting component, an inner container is arranged in the shaking tank, the shaking tank is rotationally connected with the inner container, a cell shaking chamber for shaking cells is arranged in the middle of the inner container, a test tube fixing hole is further formed in the inner container, a motor is arranged at the bottom of the shaking tank, and the output end of the motor is connected with the inner container;

the bottom of the shaking tank is provided with a storage battery.

Further, the shaking-up mechanism is inclined by 20 degrees based on a vertical angle.

Further, the output end of the motor is fixed with a transmission rotating shaft, a cross slot is formed in the inner side of the transmission rotating shaft, a transmission cross insert block is fixed at the bottom end of the inner container, and the transmission cross insert block is inserted into the cross slot on the transmission rotating shaft.

Further, the supporting component comprises a chassis, a plurality of supporting rods are uniformly distributed on the upper surface of the chassis, supporting rings are fixed on the top ends of the supporting rods, sliding rails are connected above the supporting rings in a sliding mode, rotating rings are installed on the top ends of the sliding rails, mounting frames are installed on the inner sides of the rotating rings, and shaking tanks are installed on the inner sides of the mounting frames.

Further, a tank cover is arranged at the top end of the shaking tank through a hinge.

Further, a sealing cover is arranged above the cell shaking chamber, a diversion trench is formed in the top end of the inner container, one end of the diversion trench is connected with a notch of the cell shaking chamber, and the other end of the diversion trench is located at the edge of the inner container.

The utility model has the technical effects that:

the shaking mechanism is obliquely arranged, the shaking mechanism is arranged on the supporting component, the shaking mechanism can be driven to rotate by manually rotating the rotating ring when cell liquid is shaking uniformly, and meanwhile, the cell liquid can be irregularly shaken due to the oblique arrangement of the shaking mechanism, so that the shaking is more uniform and the effect is better.

Drawings

FIG. 1 is a schematic view of the overall structure of the present utility model;

FIG. 2 is an exploded view of the structure of the support assembly of the present utility model;

fig. 3 is a schematic cross-sectional view of the shaking mechanism of the present utility model.

In the drawings, the list of components represented by the various numbers is as follows:

10. a support assembly; 11. a chassis; 12. a support rod; 13. a support ring; 14. a slide rail; 15. a rotating ring; 16. a mounting frame; 20. a shaking mechanism; 21. shaking up the tank; 22. an inner container; 23. a test tube fixing hole; 24. cell shaking chamber; 25. sealing cover; 26. a can lid; 27. a transmission cross insert block; 28. a transmission rotating shaft; 29. a motor; 30. and a storage battery.

Detailed Description

The present utility model will be specifically described with reference to examples below in order to make the objects and advantages of the present utility model more apparent. It should be understood that the following text is intended to describe only one or more specific embodiments of the utility model and does not limit the scope of the utility model strictly as claimed.

As shown in fig. 1 to 3, a shaking device for preparing cells comprises a support assembly 10 and a shaking mechanism 20, wherein the shaking mechanism 20 is installed on the inner side of the support assembly 10, and the shaking mechanism 20 is obliquely arranged;

the shaking and homogenizing mechanism 20 comprises a shaking and homogenizing tank 21, the shaking and homogenizing mechanism 20 is arranged on the inner side of the supporting component 10, an inner container 22 is arranged in the shaking and homogenizing tank 21, the shaking and homogenizing tank 21 is rotationally connected with the inner container 22, a cell shaking and homogenizing chamber 24 for shaking and homogenizing cells is arranged in the middle of the inner container 22, a test tube fixing hole 23 is further formed in the inner container 22, a motor 29 is arranged at the bottom of the shaking and homogenizing tank 21, and the output end of the motor 29 is connected with the inner container 22;

a storage battery 30 is arranged at the bottom end of the shaking-up tank 21;

when the cell shaking device is used, prepared cell liquid is injected into the cell shaking chamber 24, or a test tube injected with the cell liquid is placed into the test tube fixing hole 23 to finish fixing, then the motor 29 is started to drive the inner container 22 to rotate, the cell liquid is shaken during rotation, meanwhile, the shaking mechanism 20 can be manually controlled to integrally rotate through the supporting component 10, irregular stirring can be performed on the cell liquid, and the shaking effect is better.

Referring again to fig. 3, the shaking-up mechanism 20 is inclined by 20 ° based on the vertical angle;

in the above, the shaking-up mechanism 20 is inclined by a certain angle, and the stirring amplitude can be increased during rotation, so that the shaking-up effect is better.

Referring to fig. 2 again, a transmission rotating shaft 28 is fixed at the output end of a motor 29, a cross slot is formed in the inner side of the transmission rotating shaft 28, a transmission cross insert 27 is fixed at the bottom end of the liner 22, and the transmission cross insert 27 is inserted into the cross slot on the transmission rotating shaft 28;

the inner container 22 may have different specifications, and when the inner container 22 needs to be replaced, the inner container 22 is pulled out upwards, so that the transmission cross insert 27 at the bottom end of the inner container 22 is pulled out from the transmission rotating shaft 28, and the inner container 22 can be removed for replacement.

Referring to fig. 1 and 3, the supporting assembly 10 includes a chassis 11, a plurality of supporting rods 12 are uniformly distributed on the upper surface of the chassis 11, supporting rings 13 are fixed at the top ends of the supporting rods 12, sliding rails 14 are slidably connected above the supporting rings 13, a rotating ring 15 is mounted at the top ends of the sliding rails 14, a mounting frame 16 is mounted at the inner side of the rotating ring 15, and a shaking tank 21 is mounted at the inner side of the mounting frame 16;

above-mentioned, when carrying out the cell sap and shaking evenly, accessible holds the swivel ring 15 to swivel ring 15, drive shake even mechanism 20 and rotate, make the cell sap shake even time can carry out irregular stirring, make the cell sap shake even effect better.

Referring again to fig. 1, the top end of the shaking-up tank 21 is hinged with a tank cover 26;

above-mentioned, when the cell shakes evenly, cover jar lid 26 in shaking even jar 21 top, jar lid 26 can press on the test tube, avoids the test tube not hard up, accomplishes the fixed of test tube.

Referring to fig. 3 again, a sealing cover 25 is mounted above the cell shaking chamber 24, a diversion trench is formed at the top end of the liner 22, one end of the diversion trench is connected with the notch of the cell shaking chamber 24, and the other end of the diversion trench is located at the edge of the liner 22;

the sealing cover 25 can prevent the cell liquid in the cell shaking chamber 24 from leaking during shaking, and the diversion trench provided in the liner 22 can divert the cell liquid when pouring the cell liquid after shaking the cell liquid.

The working principle of the utility model is as follows: when preparing cells, the mixed cell liquid is injected into the test tube or directly injected into the cell shaking chamber 24, if the mixed cell liquid is injected into the test tube, the test tube is sealed, the test tube is placed in the test tube fixing hole 23, if the mixed cell liquid is directly injected into the cell shaking chamber 24, the test tube is covered on the sealing cover 25, then the tank cover 26 is covered, the motor 29 is started, the transmission rotating shaft 28 is started to rotate through the motor 29, the inner container 22 is driven to rotate when the transmission rotating shaft 28 rotates, the cell liquid can be stirred when the inner container 22 rotates, meanwhile, besides the cell liquid is stirred when the inner container 22 rotates, the rotating ring 15 is manually rotated, the shaking mechanism 20 is driven to integrally rotate, the inclination direction of the shaking mechanism 20 is changed, the shaking amplitude of the cell liquid is increased, the cell liquid can be subjected to large-amplitude irregular shaking, the shaking effect is better, and the cell preparation speed is higher.

The foregoing is merely a preferred embodiment of the present utility model and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present utility model, which are intended to be comprehended within the scope of the present utility model. Structures, devices and methods of operation not specifically described and illustrated herein, unless otherwise indicated and limited, are implemented according to conventional means in the art.

Claims

1. Shaking device for cell preparation, which is characterized in that: the device comprises a supporting component (10) and a shaking mechanism (20), wherein the shaking mechanism (20) is arranged on the inner side of the supporting component (10), and the shaking mechanism (20) is obliquely arranged;

the shaking and homogenizing mechanism (20) comprises a shaking and homogenizing tank (21), the shaking and homogenizing mechanism (20) is arranged on the inner side of the supporting component (10), an inner container (22) is arranged in the shaking and homogenizing tank (21), the shaking and homogenizing tank (21) is rotationally connected with the inner container (22), a cell shaking and homogenizing chamber (24) for shaking and homogenizing cells is formed in the middle of the inner container (22), a test tube fixing hole (23) is further formed in the inner container (22), a motor (29) is arranged at the bottom of the shaking and homogenizing tank (21), and the output end of the motor (29) is connected with the inner container (22);

the bottom end of the shaking-up tank (21) is provided with a storage battery (30).

2. The shaking device for preparing cells according to claim 1, wherein: the shaking-up mechanism (20) is inclined by 20 degrees based on a vertical angle.

3. The shaking device for preparing cells according to claim 1, wherein: the output end of the motor (29) is fixedly provided with a transmission rotating shaft (28), a cross slot is formed in the inner side of the transmission rotating shaft (28), a transmission cross insert block (27) is fixed at the bottom end of the inner container (22), and the transmission cross insert block (27) is inserted into the cross slot on the transmission rotating shaft (28).

4. The shaking device for preparing cells according to claim 1, wherein: the supporting assembly (10) comprises a chassis (11), a plurality of supporting rods (12) are uniformly distributed on the upper surface of the chassis (11), supporting rings (13) are fixed on the top ends of the supporting rods (12), sliding rails (14) are connected above the supporting rings (13) in a sliding mode, rotating rings (15) are installed on the top ends of the sliding rails (14), mounting frames (16) are installed on the inner sides of the rotating rings (15), and shaking tanks (21) are installed on the inner sides of the mounting frames (16).

5. The shaking-up device for cell preparation of claim 4, wherein: the top end of the shaking-up tank (21) is provided with a tank cover (26) through a hinge.

6. The shaking-up device for cell preparation according to claim 2, wherein: the cell shaking chamber (24) is characterized in that a sealing cover (25) is arranged above the cell shaking chamber (24), a diversion trench is formed in the top end of the inner container (22), one end of the diversion trench is connected with a notch of the cell shaking chamber (24), and the other end of the diversion trench is located at the edge of the inner container (22).