CN219166480U

CN219166480U - Stem cell culture's sample collection equipment

Info

Publication number: CN219166480U
Application number: CN202223144935.6U
Authority: CN
Inventors: 严林俊; 袁拥军; 郑雅文
Original assignee: Nantong Vocational College Science and Technology
Current assignee: Nantong Vocational College Science and Technology
Priority date: 2022-11-25
Filing date: 2022-11-25
Publication date: 2023-06-13
Anticipated expiration: 2032-11-25

Abstract

The utility model relates to the technical field of medical instruments and discloses sample collection equipment for stem cell culture, which comprises a sleeve, wherein the inner side of the sleeve is movably connected with a round rod through a bearing, a fixed ring is arranged below the sleeve, a collection mechanism is arranged on the inner side of the fixed ring, and an auxiliary mechanism is arranged at the top of the collection mechanism. This stem cell culture's sample collection equipment, through setting up collection mechanism, insert the syringe needle in the skin during the collection, through syringe needle and feed liquor pipe to the downthehole stem cell of taking out of suction, the extraction is accomplished and is rotated 90 degrees with the round bar, new suction hole bit in this moment is located the feed liquor pipe top, stem cells in different positions can be taken out, need not to change equipment, convenient operation is swift, the work efficiency of stem cell collection has been improved, in addition, can dismantle the fixed block under the effect of fixture block, draw-in groove and pull rod, clear up collection equipment is inside, can dismantle solid fixed ring under screwed pipe and screwed groove effect, wash its inside.

Description

Stem cell culture's sample collection equipment

Technical Field

The utility model relates to the technical field of medical equipment, in particular to sample collection equipment for stem cell culture.

Background

Stem cells are a class of cells with unlimited or immortalized self-renewing capacity, capable of producing at least one type of highly differentiated daughter cells, a class of primitive undifferentiated cells with multipotent differentiation and self-replicative capacity, primitive cells forming various tissue organs of mammals, stem cells have morphological commonalities, usually circular or oval, small cell volumes, relatively large nuclei, mostly euchromatin, and higher nuclei; stem cells need to be collected when culturing or experiments are performed, and sample collection equipment is needed when collecting the stem cells.

Most of the existing stem cell sample collection devices are single needle tubes, multiple groups of needle tubes are needed to be used when stem cells at different positions are collected, operation is troublesome, most of the existing stem cell sample collection devices are of an integral structure, the existing stem cell sample collection devices cannot be detached and cleaned after being used, the existing stem cell sample collection devices cannot be reused, and resource waste is caused.

Disclosure of Invention

The utility model aims to provide a sample collection device for stem cell culture, which solves the problems in the background art.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a sample collection equipment of stem cell culture, includes the sleeve pipe, the sleeve pipe inboard has the round bar through bearing swing joint, the sleeve pipe below is provided with the solid fixed ring, the solid fixed ring inboard is provided with collection mechanism, collection mechanism top is provided with assist mechanism.

The collecting mechanism comprises a fixed block, the fixed block is arranged on the inner side of a fixed ring, the top of the fixed block is fixedly connected with a liquid inlet pipe, a thread groove is formed in the top of the fixed ring, the bottom end of a sleeve is fixedly connected with a thread pipe, a liquid suction hole is formed in the top end of a round rod, a piston is slidably connected on the inner side of the liquid suction hole, the top of the piston is fixedly connected with a pull pipe, and the bottom end of the liquid inlet pipe is in threaded connection with a needle head.

Preferably, the screwed pipe is connected with the inner side of the screwed groove, and the top end of the liquid inlet pipe is tightly attached to the bottom end of the round rod, so that the liquid suction hole is in contact with the liquid inlet pipe, and leakage of stem cells is avoided.

Preferably, the handle is fixedly connected with the top end of the pull tube, the limiting ring is slidably connected with the outer surface of the pull tube, the limiting ring is fixedly connected with the inner side of the liquid suction hole, and the handle conveniently pulls the pull tube to move upwards, so that the piston is driven to move upwards.

Preferably, grooves are formed in the left side and the right side of the fixing ring, springs are fixedly connected to the left side in the grooves, clamping blocks are fixedly connected to the right ends of the springs, and the springs can enable the clamping blocks to reset and enter the clamping grooves.

Preferably, the draw-in groove has all been seted up to the fixed block left and right sides, the fixture block right side is located the draw-in groove inboard, the fixture block bottom sets up to the inclined plane, and the setting up of inclined plane makes things convenient for the fixture block to enter into in the draw-in groove, and fixture block and draw-in groove are spacing to the fixed block.

Preferably, the left side fixedly connected with pull rod of fixture block, the pull rod left end runs through in the recess left side and extends to outside the solid fixed ring, the drawing liquid hole is provided with four, and four drawing liquid holes are annular distribution at the round bar top, and the pull rod can stimulate the fixture block and remove, and four drawing liquid holes can extract the stem cell of different positions.

Preferably, the auxiliary mechanism comprises a liquid outlet pipe, the liquid outlet pipe is fixedly connected to the top of the fixed block, and the bottom end of the liquid outlet pipe penetrates through the fixed block and extends out of the fixed ring.

Preferably, the handle top is provided with an air inlet hole, the bottom end of the air inlet hole is communicated with the top of the pull tube, the bottom of the piston is provided with a through hole, the top end of the through hole is communicated with the bottom of the pull tube, and the air inlet hole is convenient for air to enter.

Compared with the prior art, the utility model has the beneficial effects that:

1. this stem cell culture's sample collection equipment, through setting up collection mechanism, in the use, the extraction is accomplished with the round bar 90 degrees of rotation, and new pumping hole is located in feed liquor pipe top this time, can extract the stem cell of different positions, need not to change equipment, convenient operation is swift, has improved stem cell collection's work efficiency, in addition, can dismantle the fixed block under the effect of fixture block, draw-in groove and pull rod, clear up collection equipment inside, can dismantle solid fixed ring under screwed pipe and screw groove effect, washs its inside.

2. This stem cell culture's sample collection equipment, through setting up assist mechanism, in the use, when the stem cell that will gather is taken out, when rotatory to drain pipe top with the suction hole after carrying, thereby the stopper at pulling handle top, thereby the taking out of stopper makes the air enter into the suction hole through inlet port and pull pipe and through-hole, the air enters into the suction downthehole piston that makes of suction, stem cell in the suction downthehole passes through the drain pipe discharge, can put into different culture vessel with the stem cell of different positions in, further improved the work efficiency that stem cell gathered, the practicality of the device has further been improved under the cooperation with collection mechanism.

Drawings

FIG. 1 is a schematic diagram of a front side of the overall structure of the present utility model;

FIG. 2 is a schematic cross-sectional view of a front portion of the overall structure of the present utility model;

FIG. 3 is a schematic view in partial cross-section of a pull tube according to the present utility model;

fig. 4 is an enlarged schematic view of the structure at a in fig. 2.

In the figure: 1. a sleeve; 2. a collection mechanism; 201. pulling a tube; 202. a limiting ring; 203. a liquid suction hole; 204. a piston; 205. a liquid inlet pipe; 206. a needle; 207. a threaded tube; 208. a thread groove; 209. a groove; 210. a spring; 211. a pull rod; 212. a clamping groove; 213. a clamping block; 214. a fixed block; 215. a handle; 3. an auxiliary mechanism; 301. a plug; 302. a liquid outlet pipe; 303. a through hole; 4. a fixing ring; 5. a round rod.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

The utility model provides the following technical scheme:

example 1

Referring to fig. 1-4, a sample collection device for stem cell culture comprises a sleeve 1, wherein a round rod 5 is movably connected to the inner side of the sleeve 1 through a bearing, a fixed ring 4 is arranged below the sleeve 1, a collection mechanism 2 is arranged on the inner side of the fixed ring 4, and an auxiliary mechanism 3 is arranged at the top of the collection mechanism 2.

The collection mechanism 2 includes fixed block 214, fixed block 214 sets up in fixed ring 4 inboard, fixed block 214 top fixedly connected with feed liquor pipe 205, threaded groove 208 has been seted up at fixed ring 4 top, sleeve pipe 1 bottom fixedly connected with screwed pipe 207, drawing liquid hole 203 has been seted up on round bar 5 top, drawing liquid hole 203 inboard sliding connection has piston 204, piston 204 top fixedly connected with draw tube 201, feed liquor pipe 205 bottom threaded connection has syringe needle 206, the extraction is accomplished and is rotated 90 degrees round bar, new drawing liquid hole bit in this moment is located the feed liquor pipe top, stem cells in different positions can be extracted, need not to change equipment, convenient operation is swift, the work efficiency of stem cell collection has been improved.

Further, the threaded pipe 207 is in threaded connection with the inner side of the threaded groove 208, the top end of the liquid inlet pipe 205 is tightly attached to the bottom end of the round rod 5, the handle 215 is fixedly connected with the top end of the pull pipe 201, the limiting ring 202 is slidably connected with the outer surface of the pull pipe 201, the limiting ring 202 is fixedly connected with the inner side of the liquid suction hole 203, and the fixing block 214 can be taken down under the action of the threaded pipe 207 and the threaded groove 208.

Further, grooves 209 are formed in the left side and the right side in the fixing ring 4, springs 210 are fixedly connected to the left side in the grooves 209, clamping blocks 213 are fixedly connected to the right ends of the springs 210, clamping grooves 212 are formed in the left side and the right side of the fixing blocks 214, the right sides of the clamping blocks 213 are located on the inner sides of the clamping grooves 212, inclined surfaces are arranged at the bottoms of the clamping blocks 213, and the liquid inlet pipe 205 and the liquid outlet pipe 302 can be taken out to clean the inside of the device under the action of the clamping blocks 213 and the clamping grooves 212.

Further, fixture block 213 left side fixedly connected with pull rod 211, the pull rod 211 left end runs through in recess 209 left side and extends outside solid fixed ring 4, and the drawing liquid hole 203 is provided with four, and four drawing liquid holes 203 are annular distribution at round bar 5 top, in addition, can dismantle fixed block 214 under fixture block 213, draw-in groove 212 and pull rod 211's effect, clear up collection equipment inside, can dismantle solid fixed ring 4 under screwed pipe 207 and screw groove 208 effect, washs its inside.

Example two

With reference to fig. 1-4, the auxiliary mechanism 3 further includes a liquid outlet pipe 302, where the liquid outlet pipe 302 is fixedly connected to the top of the fixed block 214, and the bottom end of the liquid outlet pipe 302 penetrates through the fixed block 214 and extends out of the fixed ring 4.

Further, the top of the handle 215 is provided with an air inlet, the bottom of the air inlet is communicated with the top of the pull tube 201, the bottom of the piston 204 is provided with a through hole 303, the top of the through hole 303 is communicated with the bottom of the pull tube 201, the plug 301 is taken out to enable air to enter the liquid suction hole 203 through the air inlet and the pull tube 201 and the through hole 303, the air enters the liquid suction hole 203 to enable the piston 204 to lose suction, stem cells in the liquid suction hole 203 are discharged through the liquid outlet pipe 302, and the stem cells in different positions can be placed into different culture containers, so that the working efficiency of stem cell collection is further improved, and the practicability of the device is further improved under the cooperation of the device and the collection mechanism 2.

In the actual operation process, when the device is used, the needle 206 is inserted into the skin during collection, the piston 204 is driven to move upwards through the handle 215 and the pull tube 201, stem cells are extracted into the liquid extraction hole 203 through the needle 206 and the liquid inlet tube 205, the round rod 5 is rotated by 90 degrees after the extraction is completed, and the new liquid extraction hole 203 is positioned above the liquid inlet tube 205, so that the stem cells at different positions can be extracted without changing equipment;

when the collected stem cells need to be taken out, the carried liquid suction hole 203 is rotated to the position above the liquid outlet pipe 302, the plug 301 at the top of the handle 215 is pulled, the plug 301 is taken out, so that air enters the liquid suction hole 203 through the air inlet hole, the pull pipe 201 and the through hole 303, the air enters the liquid suction hole 203, the piston 204 loses suction, and the stem cells in the liquid suction hole 203 are discharged through the liquid outlet pipe 302.

It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises an element.

Claims

1. A sample collection device for stem cell culture, comprising a cannula (1), characterized in that: the inner side of the sleeve (1) is movably connected with a round rod (5) through a bearing, a fixed ring (4) is arranged below the sleeve (1), an acquisition mechanism (2) is arranged on the inner side of the fixed ring (4), and an auxiliary mechanism (3) is arranged at the top of the acquisition mechanism (2);

collection mechanism (2) are including fixed block (214), fixed block (214) set up in fixed ring (4) inboard, fixed block (214) top fixedly connected with feed liquor pipe (205), thread groove (208) have been seted up at fixed ring (4) top, sleeve pipe (1) bottom fixedly connected with screwed pipe (207), drawing liquid hole (203) have been seted up on pole (5) top, drawing liquid hole (203) inboard sliding connection has piston (204), piston (204) top fixedly connected with draw tube (201), feed liquor pipe (205) bottom threaded connection has syringe needle (206).

2. The stem cell culture sample collection apparatus of claim 1, wherein: the threaded pipe (207) is in threaded connection with the inner side of the threaded groove (208), and the top end of the liquid inlet pipe (205) is tightly attached to the bottom end of the round rod (5).

3. The stem cell culture sample collection apparatus of claim 1, wherein: the top end of the pull tube (201) is fixedly connected with a handle (215), the outer surface of the pull tube (201) is slidably connected with a limiting ring (202), and the limiting ring (202) is fixedly connected to the inner side of the liquid suction hole (203).

4. The stem cell culture sample collection apparatus of claim 1, wherein: the left side and the right side in the fixed ring (4) are both provided with grooves (209), a spring (210) is fixedly connected to the left side in the grooves (209), and a clamping block (213) is fixedly connected to the right end of the spring (210).

5. The stem cell culture sample collection apparatus of claim 4, wherein: clamping grooves (212) are formed in the left side and the right side of the fixing block (214), the right side of the clamping block (213) is located on the inner side of the clamping groove (212), and the bottom of the clamping block (213) is provided with an inclined plane.

6. The stem cell culture sample collection apparatus of claim 4, wherein: the clamping block (213) left side fixedly connected with pull rod (211), pull rod (211) left end runs through in recess (209) left side and extends to outside solid fixed ring (4), drawing liquid hole (203) are provided with four, and four drawing liquid holes (203) are annular distribution at round bar (5) top.

7. The stem cell culture sample collection apparatus of claim 1, wherein: the auxiliary mechanism (3) comprises a liquid outlet pipe (302), the liquid outlet pipe (302) is fixedly connected to the top of the fixed block (214), and the bottom end of the liquid outlet pipe (302) penetrates through the fixed block (214) and extends out of the fixed ring (4).

8. A stem cell culture sample collection apparatus according to claim 3, wherein: an air inlet hole is formed in the top of the handle (215), the bottom end of the air inlet hole is communicated with the top of the pull tube (201), a through hole (303) is formed in the bottom of the piston (204), and the top end of the through hole (303) is communicated with the bottom of the pull tube (201).