CN219126833U

CN219126833U - Convenient harmless mouse fixing device

Info

Publication number: CN219126833U
Application number: CN202223133700.7U
Authority: CN
Inventors: 申祖琦; 李蔚; 常伟琪; 俞瑾; 王彦青; 毛应启梁
Original assignee: Fudan University
Current assignee: Fudan University
Priority date: 2022-11-25
Filing date: 2022-11-25
Publication date: 2023-06-06
Anticipated expiration: 2032-11-25

Abstract

The utility model discloses a convenient harmless mouse fixing device, which comprises a barrel and a screw cap, wherein the barrel is hollow, one end of the barrel is provided with an inlet and is in threaded connection with the screw cap, the other end of the barrel is a conical part, and the end part of the barrel is provided with an air hole; 2 forelimb placing holes and 2 hindlimb placing holes are formed in one side of the barrel, and a square hole is formed in the other side of the barrel. According to the centrifuge tube barrel body wrapped in a surrounding mode without compression, the exploratory psychology of the preferred holes of the mice is utilized, the mice are willing to drill into the barrel body through the opening at the front end of the cone, and meanwhile, the breath of the mice is facilitated, and the compression choking possibly caused by the fixation of teeth, mouth and nose is avoided. The rectangular cross section opening at toper portion and cylinder juncture then helps the head to e.g. relevant operation such as electric stimulation, formation of image, dosing, can use manpower sparingly, can alone carry out follow-up experimental operation after the fixed. And here the opening is larger, the operable space increases in the case of a good fixation.

Description

Convenient harmless mouse fixing device

Technical Field

The utility model relates to the technical field of laboratory animal experiment devices, in particular to a convenient harmless mouse fixing device.

Background

At present, mice are the most commonly used animal models in neurobiological research, and clinical experimental research is mostly needed to be carried out under the condition that animals are awake, such as animal model production inducing anxiety and depression after long-term restraint, in vivo imaging and electroencephalogram recording, administration in brain areas, electric needle intervention and the like.

At present, a foam board and an adhesive tape or an elastic band are generally used for fixing a laboratory mouse, the four limbs of the mouse are adhered to the foam board by the adhesive tape or are fastened and fixed by the elastic band along a diagonal line, the fixing method is complex in operation, high in requirements on proficiency and experience of operators, low in success rate, nonuniform in intervention in the same group, unfavorable for observation of experimental results, and extremely large in stimulation, is not suitable for research of neuropsychiatric diseases, and in addition, the mouse adhered too loosely is easy to escape, and the mouse struggled too tightly can cause limb malposition and death.

In addition, the head of the mouse is usually fixed under deep anesthesia after the anesthetic is injected into the abdominal cavity, and the head of the mouse is fixed by three points through two ears and teeth, so that the skill and the skill of an operator are required to be high, and the mouse can die due to pressing of the medulla oblongata or the follow-up experiment fails due to unsuccessful fixation. Under deep anesthesia, partial brain area and neuron activities of the mice are in an inhibition state, so that the mice are not suitable for the requirements of neuropsychiatric study on the awake state, and the mice can struggle vigorously in the anesthesia awake stage and the awake state, so that effective operation is difficult to realize, and stress and injury can be caused to the mice, even disability is caused.

The disadvantages of the prior art are mainly:

1. complicated use, high requirements on the manipulation and the proficiency of operators, and difficulty in realizing the uniformity of the fixation among groups

2. The fixed stimulation is too large in the awake state, and is not suitable for researching the neuropsychiatric diseases.

Disclosure of Invention

In order to overcome the defects in the prior art, the utility model provides a convenient harmless mouse fixing device. The harmless fixing device for the laboratory awake mice just fills the blank, is convenient for carrying out the administration operation of the electric needle and the brain area on the mice, and can provide convenience for in-vivo electrophysiology and in-vivo imaging operation.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

the convenient harmless mouse fixing device comprises a barrel and a screw cap, wherein the barrel is hollow, one end of the barrel is provided with an inlet and is in threaded connection with the screw cap, the other end of the barrel is a conical part, and the end part of the barrel is provided with an air hole; 2 forelimb placing holes and 2 hindlimb placing holes are formed in one side of the barrel, and a square hole is formed in the other side of the barrel.

The novel barrel also comprises a filler, wherein the filler is filled at the inlet part of the barrel.

The spiral cover is provided with a round hole for the tail to pass through, and the diameter of the round hole is 5mm.

The cylinder is a 50 ml centrifuge tube.

The diameter of the ventilation holes is 10mm.

The square hole size is 15mm x 5mm; the distance between the square hole and the ventilation hole is 8mm.

The radius of the forelimb placing hole is 3mm, and the distance between the two forelimb placing holes Kong Zhongdian is 8mm.

The radius of the hindlimb placing hole is 4mm, and the distance between the two hindlimb placing holes Kong Zhongdian is 10mm.

The utility model has the technical effects and advantages that:

1. through the centrifuge tube barrel body that surrounds the parcel is not pressed to utilize the exploring psychology that the mouse prefers the hole, make the mouse be willing to drill into the barrel in cone front end opening, make things convenient for its breathing simultaneously, avoided the fixed oppression choking that probably causes of tooth mouth nose.

2. The rectangular cross section opening at the junction of the cone and the cylinder is beneficial to head related operations such as electrical stimulation, imaging, drug administration and the like, can save manpower, and can be used for carrying out subsequent experimental operation by one person after fixation. And here the opening is larger, the operable space increases in the case of a good fixation.

3. The four limbs extend out of the small hole with the circular cross section of the barrel body, so that a state without an acting point is caused for the mice, the struggling force of the mice is greatly reduced, in addition, the compression and the injury of the extending barrel body to the four limbs are far lower than those of plate type fixation, and the stress of the mice is greatly reduced. Is a good fixing device in the field of neuropsychiatric disease research.

Drawings

FIG. 1 is a schematic view of a side structure of a cylinder;

FIG. 2 is a schematic view of the other side of the cylinder;

FIG. 3 is a bottom view of FIG. 1;

FIG. 4 is a schematic view of a screw cap structure;

FIGS. 5 and 6 are dimensional schematic views;

fig. 7 and 8 are schematic views of the two sides of the barrel after the mice are placed.

Detailed Description

The following description of the technical solutions in the embodiments of the present utility model will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

The convenient harmless mouse fixing device shown in the figures 1-8 comprises a barrel 1 and a spiral cover 2, wherein the barrel is hollow, one end of the barrel 1 is provided with an inlet 3 and is in threaded connection with the spiral cover 2, the other end is provided with a conical part 4, and the end part is provided with an air hole 5; 2 forelimb placing

holes

6 and 2 hindlimb placing holes 7 are formed in one side of the cylinder body 1, and a square hole 9 is formed in the other side of the cylinder body.

Preferably, a filler 8 is also included, which is filled in the inlet portion of the cylinder.

Preferably, the spiral cover 2 is provided with a round hole 21 for the tail to pass through, and the diameter of the round hole is 5mm.

Preferably, the cylinder is a 50 ml transparent centrifuge tube.

Preferably, the diameter of the ventilation holes is 10mm.

Preferably, the square hole size is 15mm x 5mm; the distance between the square hole and the ventilation hole is 8mm.

Preferably, the radius of the forelimb placement hole is 3mm, and the distance between the two forelimb placements Kong Zhongdian is 8mm.

Preferably, the diameter of the hindlimb placement hole is 4mm, and the distance between the two hindlimb placements Kong Zhongdian is 10mm.

As shown in fig. 7 and 8. When a mouse is fixed, the mouse is placed flat, the front limb placing hole and the rear limb placing hole are downward, the square small hole is upward in cross section, the spiral cover is opened, the tail of the mouse is lifted to slightly guide the tail of the mouse to enter the mouse, the kissing part stretches into the small hole at the cone of the front end of the mouse, then the mouse is erected, four limbs of the mouse respectively penetrate out of the corresponding holes, then the rest of the mouse is filled with fillers such as toilet paper or sanitary cotton balls, and the spiral cover is screwed to finish fixing.

Although the preferred embodiments of the present utility model have been described above with reference to the accompanying drawings, the present utility model is not limited to the embodiments of the above-described embodiments, and various similar changes can be made by those skilled in the art without departing from the spirit and scope of the utility model, such as: the filler is changed into wood chips, and the size of the opening and the like are all within the protection scope of the utility model.

In addition, when tail intravenous injection or related operation is needed, the filler can be placed at the front part, the space for accommodating the mice is reserved at the rear part of the reserved cylinder, and the tail part can be penetrated out from the small hole on the spiral cover to perform tail related operation. The small hole opening penetrating the tail part of the spiral cover is of a circular section and has a diameter of 5mm.

Claims

1. The convenient harmless mouse fixing device is characterized by comprising a barrel body and a screw cap, wherein the barrel body is hollow, one end of the barrel body is provided with an inlet and is in threaded connection with the screw cap, the other end of the barrel body is a conical part, and the end part of the barrel body is provided with an air hole; 2 forelimb placing holes and 2 hindlimb placing holes are formed in one side of the barrel, and a square hole is formed in the other side of the barrel.

2. A portable harmless mouse fixing device according to claim 1, wherein: the novel barrel also comprises a filler, wherein the filler is filled at the inlet part of the barrel.

3. A portable harmless mouse fixing device according to claim 1, wherein: the spiral cover is provided with a round hole for the tail to pass through, and the diameter of the round hole is 5mm.

4. A portable harmless mouse fixing device according to claim 1, wherein: the cylinder is a 50 ml centrifuge tube.

5. A portable harmless mouse fixing device according to claim 1, wherein: the diameter of the ventilation holes is 10mm.

6. A portable harmless mouse fixing device according to claim 1, wherein: the square hole size is 15mm x 5mm; the distance between the square hole and the ventilation hole is 8mm.

7. A portable harmless mouse fixing device according to claim 1, wherein: the radius of the forelimb placing hole is 3mm, and the distance between the two forelimb placing holes Kong Zhongdian is 8mm.

8. A portable harmless mouse fixing device according to claim 1, wherein: the radius of the hindlimb placing hole is 4mm, and the distance between the two hindlimb placing holes Kong Zhongdian is 10mm.