CN219099171U

CN219099171U - PCR amplification instrument

Info

Publication number: CN219099171U
Application number: CN202222764543.3U
Authority: CN
Inventors: 苏文海; 林奕明; 魏超; 魏劭; 张振刚; 郑靖曦
Original assignee: XIAMEN AMPLLY BIOTECHNOLOGY CO Ltd
Current assignee: XIAMEN AMPLLY BIOTECHNOLOGY CO Ltd
Priority date: 2022-10-20
Filing date: 2022-10-20
Publication date: 2023-05-30
Anticipated expiration: 2032-10-20

Abstract

The utility model provides a PCR amplification apparatus, comprising: the amplification instrument comprises an amplification instrument body, a heating table and a controller which are arranged in the amplification instrument body, a first temperature sensor and a second temperature sensor which are arranged in the heating table and are placed at different positions, and a prompt device arranged in the amplification instrument body; the input end of the controller is electrically connected with the first temperature sensor and the second temperature sensor, and the prompting device is electrically connected with the output end of the controller; the controller is configured to acquire a temperature value of a first position of the heating table through a first temperature sensor and acquire a temperature value of a second position of the heating table through a second temperature sensor; the prompting device is used for receiving the temperature abnormality signal output by the controller. The problem of the PCR amplification instrument among the prior art probably have its inside temperature sensor unable correct reaction heating platform's temperature after many times operation, lead to detection or experimental result unusual is solved.

Description

PCR amplification instrument

Technical Field

The utility model relates to the field of amplification instruments, in particular to a PCR amplification instrument.

Background

The polymerase chain reaction (Polymerase Chain Reaction, abbreviated as PCR) is an in vitro nucleic acid amplification technique developed in the middle of the 80 s of the last century for amplifying specific DNA fragments. It can be regarded as the special DNA replication outside the organism, and the biggest characteristic of PCR is that trace DNA can be greatly increased. The method is widely applied to detection of infectious diseases, tumors and genetic diseases in medical tests, and the most valuable fields are diagnosis of the infectious diseases, such as hepatitis B virus and new coronavirus.

The PCR amplification instrument designed at present is only provided with one temperature sensor, which can only simply read the temperature and cannot form a closed loop verification, and the accuracy of the temperature is attenuated after the temperature sensor is subjected to temperature rise and fall for thousands of times. At this time, the temperature control device can further cause inaccurate temperature control due to inaccurate temperature reading, so that when the PCR is operated and is in a high-temperature stage, the actual temperature is too high to cause the inactivation of active enzyme, and double-stranded DNA can not be effectively unwound into a single strand; at a low temperature Duan Shikong, the single strand cannot be effectively complementary paired with a base to form a double strand, so that the experimental result is abnormal.

In view of this, the present application is presented.

Disclosure of Invention

The utility model discloses a PCR (polymerase chain reaction) amplification instrument, which aims to solve the problem that the abnormal detection or experiment result is caused by the fact that the temperature of a reaction heating table cannot be correctly detected by an internal temperature sensor of the PCR amplification instrument after the PCR amplification instrument is operated for a plurality of times in the prior art.

The embodiment of the utility model provides a PCR amplification instrument, which comprises: the amplification instrument comprises an amplification instrument body, a heating table and a controller which are arranged in the amplification instrument body, a first temperature sensor and a second temperature sensor which are arranged in the heating table and are placed at different positions, and a prompt device arranged in the amplification instrument body;

the input end of the controller is electrically connected with the first temperature sensor and the second temperature sensor, and the prompting device is electrically connected with the output end of the controller;

the controller is configured to acquire a temperature value of a first position of the heating table through a first temperature sensor and acquire a temperature value of a second position of the heating table through a second temperature sensor;

the prompting device is used for receiving the temperature abnormality signal output by the controller.

Preferably, the first temperature sensor and the second temperature sensor are resistive temperature sensors.

Preferably, the model of the first temperature sensor and the second temperature sensor is pt1000.

Preferably, the prompting device is a touch screen.

Preferably, the controller is of the type STM32F103.

According to the PCR amplification instrument provided by the utility model, the controller respectively collects the temperature value of the first position of the heating table and the temperature value of the second position of the heating table through the first temperature sensor and the second temperature sensor which are arranged in the heating table and are arranged at different positions, and when the difference value between the temperature value of the first position and the temperature value of the second position is monitored to be larger than the preset value, the temperature sensor is defined to be abnormal, and the controller further prompts staff to maintain the equipment as soon as possible through the temperature abnormal signal output by the prompting device, so that the problem that the temperature sensor in the PCR amplification instrument in the prior art possibly cannot correctly react to the temperature of the heating table after multiple operation, and the result of detection or experiment is abnormal is solved.

Drawings

FIG. 1 is a schematic diagram of a sensor arrangement of a PCR amplification unit according to an embodiment of the present utility model;

FIG. 2 is a schematic block diagram of a PCR amplification unit according to an embodiment of the present utility model.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments. All other embodiments, based on the embodiments of the utility model, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the utility model. Thus, the following detailed description of the embodiments of the utility model, as presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, based on the embodiments of the utility model, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the utility model.

Specific embodiments of the present utility model will be described in detail below with reference to the accompanying drawings.

Referring to FIGS. 1 and 2, an embodiment of the present utility model provides a PCR amplification apparatus, comprising: an amplification instrument body, a heating table 3 and a controller 4 which are arranged in the amplification instrument body, a first temperature sensor 1 and a second temperature sensor 2 which are arranged in the heating table 3 and are placed at different positions, and a prompt device 5 which is arranged in the amplification instrument body;

the input end of the controller 4 is electrically connected with the first temperature sensor 1 and the second temperature sensor 2, and the prompting device 5 is electrically connected with the output end of the controller 4;

the controller 4 is configured to acquire a temperature value of a first position of the heating table 3 by the first temperature sensor 1 and a temperature value of a second position of the heating table 3 by the second temperature sensor 2;

the prompting device 5 is used for receiving the temperature abnormality signal output by the controller 4.

It should be noted that, in the existing PCR amplification apparatus, a temperature sensor is generally configured in the heating stage 3 to detect the temperature of the current heating stage 3, however, as the experiment or detection proceeds, the temperature sensor may need to be heated and cooled for thousands of times, and the temperature cannot be detected correctly, which further causes inaccuracy of temperature control, further causes that the PCR is in operation, and in a high temperature stage, the actual temperature is too high to inactivate active enzymes, and the double-stranded DNA cannot be effectively unwound into single strands; at a low temperature Duan Shikong, the single strand cannot be effectively complementary paired with a base to form a double strand, so that the experimental result is abnormal.

The temperatures of the working areas of the heating table 3 are identical or almost identical, the values collected by the first temperature sensor 1 and the second temperature sensor 2 are almost identical, and when the difference between the values is greater than a preset value, the problem of the decrease of the accuracy of one of the sensors is described.

In this embodiment, the controller 4 collects the temperature value of the first position of the heating table 3 and the temperature value of the second position of the heating table 3 through the first temperature sensor 1 and the second temperature sensor 2 configured inside the heating table 3 and placed at different positions, respectively, and defines that the temperature sensor is abnormal when the difference between the temperature value of the first position and the temperature value of the second position is monitored to be greater than a preset value, specifically, when the PCR amplification instrument performs the pid temperature control, the temperature value of a preset number of times is read every preset time period, for example, the temperature sensor value is read every 1 second for 2-3 times, the difference is made between the temperature value of the first position and the temperature value of the second position, and when the difference is greater than a given threshold value, the alarm is given through the prompting device 5, so as to prompt a worker to maintain the equipment as soon as possible, thereby solving the problem that the temperature sensor inside the PCR amplification instrument in the prior art cannot correctly react to the temperature of the heating table 3 after a plurality of times of operations, and the abnormal result of detection or experiment is caused.

In this embodiment, after the temperature abnormality is found, if the accuracy of the resistive temperature sensor is reduced, one temperature sensor may be designated as a standard, and the temperature control device is combined to perform the sectional temperature correction for the other temperature sensor.

It should be noted that the PID temperature control step may include determining, by the temperature sensor, whether the current temperature meets the target temperature value, if yes, continuing to control the temperature, if no, detecting whether the temperature control duration is reached, if yes, ending the temperature control, and if no, continuing to control the temperature.

In one possible embodiment of the utility model, the first temperature sensor 1 and the second temperature sensor 2 are resistive temperature sensors.

The resistive temperature sensor is specifically a touch sensor, and can use resistance change of metal according to temperature. This type of sensor uses several metals that supply different measurement plans, the most common metal for a temperature sensor being platinum, since it supplies a larger measurement plan. Known as platinum resistance temperature sensors. With better accuracy and longer line width tolerances. In this embodiment, the temperature of the heating table 3 can be quickly and accurately reflected by the heating table 3.

In one possible embodiment of the present utility model, the model of the first temperature sensor 1 and the second temperature sensor 2 may be pt1000.

It should be noted that, in other embodiments, the first temperature sensor 1 and the second temperature sensor 2 may also be other types of sensors, which are not limited herein, but all the solutions are within the scope of the present utility model.

In a possible embodiment of the present utility model, the prompting device 5 may be a touch screen.

It should be noted that, when the difference between the temperature value acquired by the first temperature sensor 1 and the temperature value acquired by the second temperature sensor 2 is detected to be greater than a preset value, abnormal sensor information is displayed through the touch screen, and of course, in other embodiments, a buzzer or a combination of the buzzer and an indicator light may be used to implement abnormal prompting.

In a possible embodiment of the present utility model, the model of the controller 4 may be STM32F103.

It should be noted that, the controller 4 may also use other types of sensors, which are not limited herein, but all the solutions are within the scope of the present utility model.

According to the PCR amplification apparatus provided by the utility model, the controller 4 respectively collects the temperature value of the first position of the heating table 3 and the temperature value of the second position of the heating table 3 through the first temperature sensor 1 and the second temperature sensor 2 which are arranged in the heating table 3 and are arranged at different positions, and when the difference value between the temperature value of the first position and the temperature value of the second position is monitored to be larger than a preset value, the temperature sensor is defined to be abnormal, and the controller 4 further prompts a worker to maintain equipment as soon as possible through the temperature abnormality signal output by the prompting device 5, so that the problem that the temperature sensor in the PCR amplification apparatus in the prior art cannot correctly react the temperature of the heating table 3 after a plurality of times of operation, and the abnormal detection or experiment result is caused is solved.

The above is only a preferred embodiment of the present utility model, and the protection scope of the present utility model is not limited to the above examples, and all technical solutions belonging to the concept of the present utility model belong to the protection scope of the present utility model.

Claims

1. A PCR amplification instrument, comprising: the amplification instrument comprises an amplification instrument body, a heating table and a controller which are arranged in the amplification instrument body, a first temperature sensor and a second temperature sensor which are arranged in the heating table and are placed at different positions, and a prompt device arranged in the amplification instrument body;

2. The PCR amplification instrument of claim 1, wherein the first temperature sensor and the second temperature sensor are resistive temperature sensors.

3. The PCR amplification instrument of claim 1, wherein the first temperature sensor and the second temperature sensor are model pt1000.

4. The PCR amplification instrument of claim 1, wherein the prompting device is a touch screen.

5. The PCR amplification instrument of claim 1, wherein the controller is STM32F103.