CN219070754U

CN219070754U - Mouse head fixing device for realizing functional ultrasonic imaging

Info

Publication number: CN219070754U
Application number: CN202222135015.1U
Authority: CN
Inventors: 鞠培俊; 陈剑华; 罗超
Original assignee: Shanghai Mental Health Center Shanghai Psychological Counselling Training Center
Current assignee: Shanghai Mental Health Center Shanghai Psychological Counselling Training Center
Priority date: 2022-08-15
Filing date: 2022-08-15
Publication date: 2023-05-26
Anticipated expiration: 2032-08-15

Abstract

The utility model relates to a mouse head fixing device for realizing functional ultrasonic imaging, which comprises a fixing table, a pair of fixing frames, a pair of clamping arms and a gas anesthesia fixing column, wherein the pair of fixing frames are fixedly arranged on the fixing table and are oppositely arranged, the pair of clamping arms are respectively fixed in holes on the inner sides of the fixing frames on two sides, and the gas anesthesia fixing column is fixedly arranged on the fixing table and is positioned in front of the fixing frames. The mouse head fixing device for realizing functional ultrasonic imaging can obtain stable and effective brain functional ultrasonic images and analysis results of mice, and is suitable for head functional imaging scanning of various awake and anesthetized animals. The experimental fixing device can limit the movement and rotation range of the head of the visual mouse in a conscious or anesthetized state, so that the brain activity level of the visual mouse in a task state can be studied more deeply.

Description

Mouse head fixing device for realizing functional ultrasonic imaging

Technical Field

The utility model relates to the field of clinical science research, in particular to the field of ultrasonic functional imaging of a mouse head, and specifically relates to a mouse head fixing device for realizing functional ultrasonic imaging.

Background

In clinical scientific researches (such as brain chronic disease model and neuropsychiatric disease model), mice are often selected as model animals for brain function imaging experiments due to their strong reproductive capacity, short breeding cycle, small size, easy manipulation, complete transgenic lines and mature molecular manipulation. Aiming at an ultrasonic functional imaging instrument, a head fixing device is designed and combined at the same time by adopting a mode of fixing the head of the mouse by a head frame, so that the mouse can be fixed in a waking state. In the past, anesthesia is mostly adopted for brain function research of mice in a non-task state or a task state, mainly because functional imaging signals are easily affected by head movement, and the anesthesia mode can control the head movement of the mice within an ideal scale. However, the anesthesia state tends to change the activity level of the whole brain, which results in a low signal-to-noise ratio of the obtained image (small change of blood oxygen in the inhibition state), and a difficult comparison of the results from different researchers (due to different anesthetic drugs and concentrations, etc.). The study method of anesthesia is also not applicable to the study of task brain activity level. Therefore, the head of the experimental mouse is usually fixed by adopting the fixing device, so that the defect that the movement of the head of the mouse is limited in a conscious state of the mouse is ensured.

Disclosure of Invention

The utility model aims to overcome the defects of the prior art and provide the mouse head fixing device which has the advantages of simple structure, simplicity and convenience in operation and wider application range and can realize functional ultrasonic imaging.

In order to achieve the above object, the mouse head fixing device for realizing functional ultrasonic imaging of the present utility model is as follows:

the mouse head fixing device for realizing functional ultrasonic imaging is mainly characterized by comprising a fixing table, a pair of fixing frames, a pair of clamping arms and a gas anesthesia fixing column, wherein the fixing frames are fixedly arranged on the fixing table and are oppositely arranged, the clamping arms are respectively fixed in holes on the inner sides of the fixing frames on two sides, and the gas anesthesia fixing column is fixedly arranged on the fixing table and is positioned in front of the fixing frames.

Preferably, a plurality of holes are arranged on the fixing frame from top to bottom, the holes are positioned at different heights, and the clamping arms are arranged on the fixing frame through the holes.

Preferably, the gas anesthesia fixing column is provided with a movable fixing groove, and the movable fixing groove supports adjustment of different heights.

Preferably, the movable fixing groove is provided with a groove, and the groove is connected with a gas-flax device.

Preferably, the fixing table is provided with a plurality of mounting holes from front to back, and the gas anesthesia fixing column is mounted on the fixing table through the mounting holes.

Preferably, the fixing table is divided into a front end fixing table and a rear end net-shaped fixing table, the front side of the rear end net-shaped fixing table is fixedly connected to the rear side of the front end fixing table, and the middle area of the rear end net-shaped fixing table is of a net-shaped structure.

Preferably, the device further comprises a base and a support column, wherein the front end fixing table and the rear end net-shaped fixing table are fixed on the base through the support column.

Preferably, the clamping arms have a plurality of specifications with different thicknesses and different lengths, and the clamping arms with the plurality of specifications can be mounted on the fixing frame.

The mouse head fixing device for realizing functional ultrasonic imaging can obtain stable and effective brain functional ultrasonic images and analysis results of mice, and is suitable for head functional imaging scanning of various awake and anesthetized animals. The experimental fixing device is used for effectively carrying out various states (including wakefulness or anesthesia) of the mice, is suitable for scanning by an ultrasonic functional imaging instrument, and ensures the definition of scanned images. The device can limit the movement and rotation range of the head of the visually-obtained mouse under the awake or anesthetized state, so that the brain activity level of the visually-obtained mouse under the task state can be studied more deeply.

Drawings

Fig. 1 is a perspective view of a murine head fixation device of the present utility model that implements functional ultrasound imaging.

Fig. 2 is another perspective view of a murine head fixation device of the present utility model that implements functional ultrasound imaging.

Fig. 3 is a top view of a murine head fixation device of the present utility model that implements functional ultrasound imaging.

Fig. 4 is a front view of a murine head fixation device of the present utility model that implements functional ultrasound imaging.

Fig. 5 is a left side view of a murine head fixation device of the present utility model that implements functional ultrasound imaging.

Fig. 6 is a right side view of a murine head fixation device of the present utility model that implements functional ultrasound imaging.

Reference numerals:

1. front end fixing table

2. Rear end net-shaped fixing table

3. Fixing frame

4. Clamping arm

5. Gas anesthesia fixing column

6. Air-hemp device

7. Base seat

8. Support column

9. Holes and holes

10. Movable fixing groove

11. Mounting hole

Detailed Description

In order to more clearly describe the technical contents of the present utility model, a further description will be made below in connection with specific embodiments.

The mouse head fixing device for realizing functional ultrasonic imaging comprises a fixing table, a pair of fixing frames 3, a pair of clamping arms 4 and a gas anesthesia fixing column 5, wherein the pair of fixing frames 3 are fixedly arranged on the fixing table and are oppositely arranged, the pair of clamping arms 4 are respectively fixed in holes 9 on the inner sides of the fixing frames 3 on two sides, and the gas anesthesia fixing column 5 is fixedly arranged on the fixing table and is positioned in front of the fixing frames 3.

As a preferred embodiment of the present utility model, the fixing frame 3 is provided with a plurality of holes 9 from top to bottom, the holes 9 are located at different heights, and the clamping arm 4 is mounted on the fixing frame 3 through the holes 9. The holes 9 are uniform in size. The fixing requirement of the mice is met by moving the clamping arm 4 left and right or up and down.

As a preferred embodiment of the present utility model, the gas anesthesia fixing column 5 is provided with a movable fixing groove 10, and the movable fixing groove 10 supports adjustment of different heights.

As a preferred embodiment of the present utility model, the movable fixing groove 10 is provided with a groove, and the groove is connected with the air hemp device 6.

As a preferred embodiment of the present utility model, the fixing table is provided with a plurality of mounting holes 11 arranged from front to rear, and the gas anesthesia fixing column 5 is mounted on the fixing table through the mounting holes 11.

As a preferred embodiment of the present utility model, the fixing table is divided into a front end fixing table 1 and a rear end net-shaped fixing table 2, wherein the front side of the rear end net-shaped fixing table 2 is fixedly connected to the rear side of the front end fixing table 1, and the middle area of the rear end net-shaped fixing table 2 is in a net-shaped structure.

As a preferred embodiment of the present utility model, the device further comprises a base 7 and a support column 8, wherein the front end fixing table 1 and the rear end net-shaped fixing table 2 are fixed on the base 7 through the support column 8.

As a preferred embodiment of the present utility model, the clamping arms 4 have a plurality of specifications with different thicknesses and different lengths, and the clamping arms 4 with the plurality of specifications can be mounted on the fixing frame 3.

In the specific embodiment of the utility model, a mouse head fixing device suitable for ultrasonic functional imaging is disclosed, and comprises a base 7, a standby height-adjustable gas anesthesia fixing column 5, a front end fixing table 1, a rear end net-shaped fixing table 2, a liftable fixing frame 3, a clamping arm 4 and the like. The fixing of the mice can be realized through simple steps, and the unlocking is also very quick. The utility model aims to provide a mouse head fixing device for ultrasonic functional imaging, which can limit the movement and rotation of the head of a conscious mouse after fixing.

The utility model relates to a mouse head fixing device for realizing ultrasonic functional imaging, which comprises a pair of clamping arms 4 fixed by screws, a lifting type fixing frame 3, a standby air-hemp interface with adjustable height, two connected bearing fixing tables and a base 7. The connecting part is two fixing frames 3 and comprises four fixing holes 9, which respectively meet the fixing requirements of mice with different fixing heights and sizes. The clamping arms 4 are fixed through the screws of the fixing frame 3 of the connecting part in a rotating way, and different lengths are respectively adjusted to meet the fixing requirements of different types of headstock and different sizes of mice.

The gas anesthesia fixing column 5 comprises a movable fixing groove 10, and the movable fixing groove 10 can adjust different heights and front and back distances according to requirements. The movable fixing groove 10 is provided with a groove connected with the air-hemp device 6, so that the air-hemp channel can be conveniently moved back and forth. The net-shaped fixing table 2 at the rear end is convenient for behavior measurement such as drug administration, pain measurement and the like of awake animals, and the net-shaped fixing device is made of metal materials. The front end fixing table 1 and the rear end net-shaped fixing table 2 are fixed on a fixing plate of the ultrasonic function testing instrument through fixing holes.

In experiments using mice as model animals, it is often necessary to fix the head of the mice using a fixing device. Fig. 1 and 2 show perspective views of the present mouse head fixation device in one embodiment, in one or more embodiments, the mouse head fixation device includes a pair of clamp arms 4, and a connection portion connecting the pair of clamp arms 4. The clamping arm 4 and the connecting part are fixed by screw rotation (3 mm).

Fig. 4 is a schematic front view of the mouse head-fixing device of fig. 1, and fig. 3 is a schematic top view of the mouse head-fixing device of fig. 1. As can be seen from fig. 2, the free ends of the pair of clamping arms 4 are further provided with fixing parts respectively, the fixing parts are fixing frames 3 fixed at the left and right sides of the free ends of the clamping arms 4, and the fixing frames 3 are fixing elements of the mouse head fixing device. The center of one end of the fixing part is also respectively provided with a fixing hole (3 mm), and the mouse head fixing device is fixed through the screw by the fixing holes.

The side screws of the joint of the pair of clamping arms 4 and the fixing frame 3 are fixed. The experimental mice after being fixed by the clamping arms 4 of the mouse head fixing device of the utility model have the advantages that the rotation and movement range of the head movement is zero on the premise of ensuring that the mice are in a completely awake state. The head of the mouse is fixed under the condition that the normal activity level of the whole brain of the mouse can be ensured, so that the head movement of the mouse is still in an ideal range even in a task state involving various stimuli.

The rat head was shaved and fixed directly. The rat head is connected with a gas anesthesia device for fixing gas anesthesia. When the mouse head fixing device is used for fixing the head of an experimental mouse, the fixing surface of the mouse head fixing device is flat.

The mice were subjected to a headstock mounting procedure prior to fixation, and the skin of the mice head was opened and trimmed off according to the usual procedure, with the skull exposed as required and completely. Careful removal of the muscle tissue thereon with sharp forceps and surgical scissors is required. To ensure firm adhesion, the periosteum and other connective tissue overlying the skull also need to be carefully removed with a surgical blade. If the head of the mouse has bleeding, a hemostatic sponge is needed to compress the bleeding point for hemostasis, and then the bleeding point is wiped clean by normal saline. Before the skull is adhered to the rat head 3, the surface of the skull is kept clean and dry, and bleeding caused by subsequent operations is reduced as much as possible. And deeply stripping muscle tissues from tissues at two sides of the skull, and fixing the fixing frame 3 and the aluminum bracket on the skull by adopting tissue glue, wherein the use amount of the coated glue is as small as possible to avoid flowing to the skull.

Skull thinning or alternative surgical implementation methods. The drill bit is adopted to carefully grind bones in the area of the bones to be started, and the bones can be optionally thinned or directly ground off. During operation, the blood vessel is carefully prevented from being touched, the skull is separated by adopting a passive separation mode during skull separation, and then physiological saline is covered on the surface and hemostasis is carried out fully. The TPX film (Transparent Polymer X) was cut to an appropriate size, and after carefully secured by coating with a photocurable resin, the photocurable resin was cured using a blue LED lamp. Finally, a thicker layer of fluid glue is further applied to the contact boundary between the rat head fixing device and the rat skull to increase the adhesion.

The mouse head fixing method for ultrasonic functional imaging for achieving the purpose adopts the mouse head fixing device, and the mouse head fixing method comprises the following points according to different experimental requirements and conditions:

1. the mice were shaved, optionally with skin-cutting, and directly secured to the device of the present utility model using ear stems.

2. The skull does not replace the fixing mode of the head support. The case of skull non-replacement includes two cases: the small-age mice can be directly observed without grinding; adult mice are typically thinned.

Cleaning and sterilizing scalp; cutting off scalp, installing aluminum fixing sheets according to different depths on a customized headstock, fixing the scalp on the skull, smearing dental cement for fixing, fixing the headstock and the skull, and filling left and right gaps with 454 glue.

3. The method adopts Transparent Polymer X (TPX release film is a transparent and heat-resistant polyolefin polymer, TPX) film for skull replacement and resin fixation, and combines the fixation method of installing a head bracket.

The specific implementation manner of this embodiment may be referred to the related description in the foregoing embodiment, which is not repeated herein.

It is to be understood that the same or similar parts in the above embodiments may be referred to each other, and that in some embodiments, the same or similar parts in other embodiments may be referred to.

It should be noted that in the description of the present utility model, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Furthermore, in the description of the present utility model, unless otherwise indicated, the meaning of "plurality" means at least two.

In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present utility model. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

In this specification, the utility model has been described with reference to specific embodiments thereof. It will be apparent, however, that various modifications and changes may be made without departing from the spirit and scope of the utility model. The specification and drawings are, accordingly, to be regarded in an illustrative rather than a restrictive sense.

Claims

1. The mouse head fixing device for realizing functional ultrasonic imaging is characterized by comprising a fixing table, a pair of fixing frames, a pair of clamping arms and a gas anesthesia fixing column, wherein the fixing frames are fixedly arranged on the fixing table and are oppositely arranged, the clamping arms are respectively fixed in holes on the inner sides of the fixing frames on two sides, and the gas anesthesia fixing column is fixedly arranged on the fixing table and is positioned in front of the fixing frames.

2. The mouse head fixing device for realizing functional ultrasonic imaging according to claim 1, wherein a plurality of holes are arranged on the fixing frame from top to bottom, the holes are positioned at different heights, and the clamping arms are arranged on the fixing frame through the holes.

3. The mouse head fixing device for realizing functional ultrasonic imaging according to claim 1, wherein the gas anesthesia fixing column is provided with a movable fixing groove, and the movable fixing groove supports adjustment of different heights.

4. The mouse head fixing device for realizing functional ultrasonic imaging according to claim 3, wherein a groove is arranged on the movable fixing groove, and the groove is connected with a pneumocandin device.

5. The mouse head fixing device for realizing functional ultrasonic imaging according to claim 1, wherein a plurality of mounting holes are arranged on the fixing table from front to back, and the gas anesthesia fixing column is mounted on the fixing table through the mounting holes.

6. The mouse head fixing device for realizing functional ultrasonic imaging according to claim 1, wherein the fixing table is divided into a front end fixing table and a rear end net-shaped fixing table, the front side of the rear end net-shaped fixing table is fixedly connected to the rear side of the front end fixing table, and the middle area of the rear end net-shaped fixing table is of a net-shaped structure.

7. The mouse head fixing device for realizing functional ultrasonic imaging according to claim 6, further comprising a base and a support column, wherein the front end fixing table and the rear end net-shaped fixing table are both fixed on the base through the support column.

8. The mouse head fixing device for realizing functional ultrasonic imaging according to claim 1, wherein the clamping arms have a plurality of specifications with different thicknesses and different lengths, and the clamping arms with the plurality of specifications can be mounted on the fixing frame.