CN219058981U - Portable isothermal detection device for nucleic acid extraction - Google Patents
Portable isothermal detection device for nucleic acid extraction Download PDFInfo
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- CN219058981U CN219058981U CN202223592447.1U CN202223592447U CN219058981U CN 219058981 U CN219058981 U CN 219058981U CN 202223592447 U CN202223592447 U CN 202223592447U CN 219058981 U CN219058981 U CN 219058981U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
A portable nucleic acid extraction isothermal detection device comprising: the magnetic bead washing device comprises a material plate, a magnetic bead pyrolysis liquid cavity, a washing liquid cavity, a water absorption filter paper cavity, an eluent cavity, a reagent freeze-drying ball cavity and a test strip mounting groove are sequentially arranged from one end to the other end of the material plate, and a first magnet is arranged between the eluent cavity and the reagent freeze-drying ball cavity; a moving plate, one end of which is provided with a sample groove; the bottom plate is positioned at the vertical projection of the magnetic bead cracking liquid cavity of the material plate and provided with a second magnet, and the bottom plate is positioned at the vertical projection of the reagent freeze-drying ball cavity of the material plate and provided with a heating device; the test strip is characterized in that the material plate is connected with the bottom plate and is relatively static, the movable plate is located between the material plate and the bottom plate and slides relative to the material plate and the bottom plate, the material plate, the movable plate and the bottom plate are sequentially attached, the test strip mounting groove is formed in one side of the material plate, which faces the movable plate, and the test strip mounting groove is provided with a test strip. The device is simple to operate, low in manufacturing cost, accurate in detection result and small and flexible.
Description
Technical Field
The utility model relates to the technical field of isothermal detection, in particular to a portable isothermal detection device for nucleic acid extraction.
Background
In isothermal amplification, the prior art mainly uses:
1. the laboratory adopts a manual magnetic bead method to extract nucleic acid, and after the eluted nucleic acid is manually added into a reaction reagent, the reaction reagent is put on a fluorescent PCR instrument for amplification or a common PCR instrument for amplification, a test strip is used for detection;
2. an integrated instrument device.
The defects of the prior art are as follows:
1. laboratory operations require specific environments, specialized operators; the operation time is long, the equipment is expensive, and the outdoor operation can not be performed any more;
2. the integrated equipment is heavy and very expensive, and the matched consumable is required, so that the integrated equipment is only suitable for laboratory environment detection.
Disclosure of Invention
In view of the shortcomings of the background technology, the utility model relates to a portable isothermal detection device for nucleic acid extraction, which is simple to operate, low in manufacturing cost, accurate in detection result, small and flexible and easy to carry.
The utility model relates to a portable isothermal detection device for nucleic acid extraction, comprising:
the magnetic bead washing device comprises a material plate, wherein a magnetic bead cracking liquid cavity, a washing liquid cavity, a water-absorbing filter paper cavity, an eluent cavity, a reagent freeze-drying ball cavity and a test strip mounting groove are sequentially arranged from one end to the other end of the material plate, the magnetic bead cracking liquid cavity is used for sealing and preassembling magnetic bead cracking liquid, the washing liquid cavity is used for sealing and preassembling washing liquid, the water-absorbing filter paper cavity is used for sealing and preassembling water-absorbing filter paper, the eluent cavity is used for sealing and preassembling eluent, the reagent freeze-drying ball cavity is used for sealing and preassembling reagent freeze-drying balls, and a first magnet is arranged between the eluent cavity and the reagent freeze-drying ball cavity;
a moving plate, one end of which is provided with a sample groove;
the bottom plate is positioned at the vertical projection of the magnetic bead cracking liquid cavity of the material plate and provided with a second magnet, and the bottom plate is positioned at the vertical projection of the reagent freeze-drying ball cavity of the material plate and provided with a heating device;
the test strip mounting groove is formed in one side of the material plate, which faces the moving plate, and is provided with a test strip.
By adopting the scheme, the movable plate is gradually pulled to complete all steps of cracking, washing, water absorption, elution and amplification to detection, and the method is simple to operate, small, flexible and easy to carry.
Further, the reagent freeze-dried ball is a primer probe-containing LAMP freeze-dried ball or an RPA reagent freeze-dried ball.
By adopting the above scheme, the LAMP or RPA technique can be used for nucleic acid amplification.
Further, the material plate is fixedly connected with the bottom plate, and the material plate is connected with the bottom plate to form a channel for the movable plate to slide.
By adopting the scheme, the portable bicycle is convenient to produce and carry.
Further, the length of the moving plate is longer than any one of the length of the material plate or the base plate.
By adopting the scheme, the movable plate is convenient to pull.
Drawings
FIG. 1 is a schematic cross-sectional view of embodiment 1 of the present utility model.
Fig. 2 is a schematic structural view of embodiment 2 of the present utility model.
Reference numerals: 1. a sheet of material; 11. a magnetic bead lysate chamber; 12. a washing liquid chamber; 13. a water-absorbing filter paper cavity; 14. an eluent chamber; 15. a reagent freeze-drying ball cavity; 16. a first magnet; 17. a test strip mounting groove; 2. a moving plate; 21. a sample tank; 3. a bottom plate; 31. a second magnet; 32. a heating device; 33 connection interfaces; 4. test strips.
Detailed Description
The following description and the discussion of the embodiments of the present utility model will be made more complete and less in view of the accompanying drawings, in which it is to be understood that the utility model is not limited to the embodiments of the utility model disclosed and that it is intended to cover all such modifications as fall within the scope of the utility model.
For the purpose of facilitating an understanding of the embodiments of the present utility model, reference will now be made to the drawings, by way of example, of specific embodiments, and the various embodiments should not be construed to limit the embodiments of the utility model.
Example 1 of the present utility model is shown with reference to FIG. 1, and relates to a portable nucleic acid extraction isothermal detection device comprising a material plate 1, a moving plate 2 and a bottom plate 3.
The magnetic bead lysis solution chamber 11 is used for sealing pre-filling magnetic bead lysis solution, the washing solution chamber 12 is used for sealing pre-filling washing solution, the water absorbing filter paper chamber 13 is used for sealing pre-filling water absorbing filter paper, the eluting solution chamber 14 is used for sealing pre-filling elution solution, the reagent freeze-drying ball chamber 15 is used for sealing pre-filling reagent freeze-drying balls, a first magnet 16 is arranged between the eluting solution chamber 14 and the reagent freeze-drying ball chamber 15, the first magnet 16 is used for sucking out magnetic beads, sealing pre-filling is that each chamber of the material plate 1 is provided with a small hole towards one side of the movable plate 2, leakage is prevented, a detachable cover body is arranged on the upper side of each chamber, so that liquid can be circularly filled, the liquid can be circularly used, meanwhile, the air pressure in the chamber can be adjusted through the opening of the cover body, solution in the chamber flows down, the magnetic bead lysis solution chamber 11 and the reagent freeze-drying ball chamber 15 are used for sealing pre-drying balls, the first magnet 16 is arranged between the eluting solution chamber 14 and the reagent freeze-drying ball chamber 15, the first magnet 16 is used for sucking out magnetic beads, the sealing pre-drying ball is provided with small holes towards one side of the movable plate 2, the magnetic bead is convenient for sucking down, the reagent freeze-drying ball is convenient for sucking down, and the reagent freeze-drying ball is completely opened towards the surface of the filter paper is completely sucked down, the surface of the magnetic bead is convenient to be in the freeze-drying ball, and the freeze-drying ball is convenient to be completely sucked down by the air pressure.
One end of the movable plate 2 is provided with a sample groove 21, the opening of the sample groove 21 is slightly larger than each cavity of the material plate 1, and the length of the movable plate 2 is longer than any one of the length of the material plate 1 or the length of the bottom plate 3, so that the movable plate 2 can be conveniently operated.
The second magnet 31 is arranged at the vertical projection position of the bottom plate 3 in the magnetic bead cracking liquid cavity 11 of the material plate 1, the heating device 32 is arranged at the vertical projection position of the bottom plate 3 in the reagent freeze-drying ball cavity 15 of the material plate 1, the connecting interface 33 is arranged on one side of the bottom plate 3, the connecting interface 33 is used for externally connecting a power supply and temperature control equipment with the heating device 32, and the heating device 32 is convenient to carry without adding the power supply and the temperature control equipment, and meanwhile, the production cost is reduced.
Wherein material board 1 is connected and is static relatively with bottom plate 3, movable plate 2 is located between material board 1 and the bottom plate 3 and slides for material board 1 and bottom plate 3, material board 1, movable plate 2 laminate with bottom plate 3 in proper order, material board 1 prevents the weeping with the laminating of movable plate 2, movable plate 2 and bottom plate 3's laminating makes things convenient for the work of second magnet 31 and heating device 32, and laminating setting is portable more simultaneously. The test strip mounting groove 17 is formed in one side, facing the moving plate 2, of the material plate 1, and the test strip 4 is mounted in the test strip mounting groove 17.
The working principle of the utility model is as follows:
s1, adding a sample into a lysis solution containing magnetic beads, adding the sample into a magnetic bead lysis solution cavity 11, and adsorbing the magnetic beads with nucleic acid in a bottom plate under the action of a second magnet 31 for 3min;
s2, pulling the middle plate to the right to the position of the washing liquid cavity 12, and sealing the pre-installed washing liquid to wash the nucleic acid adsorbed with the magnetic beads for 30 seconds;
s3, pulling the middle plate to the right to the position of the water-absorbing filter paper cavity 13, sealing the pre-filled water-absorbing filter paper to absorb the excessive washing liquid, and continuing for 2min;
s4, pulling the middle plate to the right to the position of the eluent sucking cavity 14, sealing the pre-loaded eluent to elute nucleic acid adsorbed by the magnetic beads, and adsorbing the magnetic beads on the side holes under the action of the side first magnet 16, so that the eluent on the middle plate is kept free from magnetic bead residues for 4min;
s5, pulling the middle plate to the right to the position of the reagent freeze-dried ball cavity 15, sealing and pre-installing the LAMP freeze-dried ball or the RPA reagent freeze-dried ball containing the primer probe to contact the eluent, immediately dissolving, and heating for 30min after the bottom heating device 32 is heated to a specified temperature;
s6, pulling the middle plate to the right to the position of the test strip 4, enabling the water-absorbing glass fiber at the bottom of the test strip 4 to contact with the reacted long object, immediately starting the test strip 4 running plate, and observing the result after 5-6min
In the embodiment 2 of the present utility model, referring to fig. 2, on the basis of the embodiment 1, the material plate 1 is fixedly connected with the bottom plate 3, and the material plate 1 is connected with the bottom plate 3 to form a channel for sliding the moving plate 2, so that the device is convenient to produce and carry, and meanwhile, the direction is more stable when the moving plate 2 is drawn, and meanwhile, the material plate 1 is made of transparent material, so that the movement position of the lower moving plate 2 is convenient to observe.
Finally, it should be noted that: the above examples are only specific embodiments of the present utility model, and are not intended to limit the scope of the present utility model, but it should be understood by those skilled in the art that the present utility model is not limited thereto, and that the present utility model is described in detail with reference to the foregoing examples: any person skilled in the art may modify or easily conceive of the technical solution described in the foregoing embodiments, or perform equivalent substitution of some of the technical features, while remaining within the technical scope of the present disclosure; such modifications, changes or substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present utility model, and are intended to be included in the scope of the present utility model. Therefore, the protection scope of the present utility model shall be subject to the protection scope of the claims.
Claims (4)
1. A portable nucleic acid extraction isothermal detection device, comprising:
the magnetic bead washing device comprises a material plate (1), wherein a magnetic bead lysate cavity (11), a washing liquid cavity (12), a water absorption filter paper cavity (13), an eluent cavity (14), a reagent freeze-drying ball cavity (15) and a test strip mounting groove (17) are sequentially arranged from one end to the other end of the material plate (1), the magnetic bead lysate cavity (11) is used for sealing and preassembling the magnetic bead lysate, the washing liquid cavity (12) is used for sealing and preassembling the washing liquid, the water absorption filter paper cavity (13) is used for sealing and preassembling the water absorption filter paper, the eluent cavity (14) is used for sealing and preassembling the eluent, the reagent freeze-drying ball cavity (15) is used for sealing and preassembling the reagent freeze-drying ball, and a first magnet (16) is arranged between the eluent cavity (14) and the reagent freeze-drying ball cavity (15);
a moving plate (2), wherein one end of the moving plate (2) is provided with a sample groove (21);
the bottom plate (3), the vertical projection department that the bottom plate (3) is located the magnetic bead pyrolysis liquid chamber (11) of material board (1) is equipped with second magnet (31), the vertical projection department that the bottom plate (3) is located the reagent freeze-drying ball chamber (15) of material board (1) is equipped with heating device (32);
the test strip is characterized in that the material plate (1) is connected with the bottom plate (3) and is relatively static, the movable plate (2) is located between the material plate (1) and the bottom plate (3) and slides relative to the material plate (1) and the bottom plate (3), the material plate (1), the movable plate (2) and the bottom plate (3) are sequentially attached, the test strip mounting groove (17) is formed in one side, facing the movable plate (2), of the material plate (1), and the test strip (4) is mounted in the test strip mounting groove (17).
2. The portable nucleic acid extraction isothermal detection device according to claim 1, wherein: the reagent freeze-dried ball is LAMP freeze-dried ball or RPA reagent freeze-dried ball containing a primer probe.
3. The portable nucleic acid extraction isothermal detection device according to claim 1, wherein: the material plate (1) is fixedly connected with the bottom plate (3), and the material plate (1) is connected with the bottom plate (3) to form a channel for the movable plate (2) to slide.
4. The portable nucleic acid extraction isothermal detection device according to claim 1, wherein: the length of the moving plate (2) is longer than any length of the material plate (1) or the bottom plate (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223592447.1U CN219058981U (en) | 2022-12-23 | 2022-12-23 | Portable isothermal detection device for nucleic acid extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223592447.1U CN219058981U (en) | 2022-12-23 | 2022-12-23 | Portable isothermal detection device for nucleic acid extraction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219058981U true CN219058981U (en) | 2023-05-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223592447.1U Active CN219058981U (en) | 2022-12-23 | 2022-12-23 | Portable isothermal detection device for nucleic acid extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219058981U (en) |
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2022
- 2022-12-23 CN CN202223592447.1U patent/CN219058981U/en active Active
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