CN219058973U - Porous sample injection cell counter - Google Patents

Abstract

The utility model discloses a porous sample cell counter which comprises a base, wherein the upper end surface of the base is fixedly connected with a shell, a rotating shaft is rotationally connected with the shell, the end part of the rotating shaft, which is far away from the base, is fixedly connected with a separation shaft, a plurality of sample injection holes which are arranged at equal intervals are formed in the side wall of the separation shaft, a guide mechanism for assisting in guiding cells is arranged on the side wall of the separation shaft, a mounting groove is formed in the side wall of one end, which is far away from the base, of the shell, a glass cover is arranged in the mounting groove, and a mounting mechanism for mounting the glass cover is arranged between the mounting groove and the glass cover. According to the utility model, through arranging the structures such as the rotating shaft, the separating shaft and the sample injection holes, the rotating shaft is utilized to drive the separating shaft to rotate and centrifuge the culture solution on the separating shaft, the rapid sample injection of the culture solution is realized by virtue of the action of centrifugal force, and the uniform distribution sample injection of the culture solution at a plurality of sample injection ports can be realized under the action of the centrifugal force.

Description

Porous sample injection cell counter

Technical Field

The utility model relates to the technical field of cell counters, in particular to a porous sample injection cell counter.

Background

The principle of manual counting and cell counter counting is in fact the same: cell counting is to give the cultured cells a certain density so that they can grow well, and the counting result is generally expressed in terms of the number of cells per milliliter (cells/ml).

The cell technology appearance is through carrying out automatic count to the cell in the culture solution, not only efficiency is higher for artificial technology, and the precision is higher, but current single hole counter is when the count, be single Kong Jinyang, the sample rate of culture solution in getting into the feed tube is slower, the count efficiency of cytograph is lower promptly, and the cell counter of porous sample introduction can't realize the even branch appearance of culture solution when using, the culture solution only advances Kong Jinyang through wherein several when the cytograph appears inclining, make inclined position's detecting tube frequency of use higher, produce error influence to its life and cell count precision, its sample inlet is because unable realization is sealed when using simultaneously current cytograph, place for a long time and can fall into the pollution of cytograph that the dust causes, and then produce the influence to the measurement precision.

Disclosure of Invention

The utility model aims to solve the defects that in the prior art, various holes are used for sampling, sampling uniformity of each sample inlet cannot be realized, and pollution is caused by dust accumulation of a feed inlet.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

the utility model provides a porous sampling cytometer, includes the support base, the up end fixedly connected with shell of support base, the rotation is connected with the axis of rotation in the shell, the tip fixedly connected with separation axle of support base is kept away from to the axis of rotation, a plurality of equidistant sampling holes that set up have been seted up on the lateral wall of separation axle, be equipped with the guiding mechanism that is used for assisting the cell direction on the lateral wall of separation axle, the mounting groove has been seted up on the one end lateral wall of support base is kept away from to the shell, be equipped with the glass cover in the mounting groove, be equipped with the installation mechanism that is used for glass cover installation between mounting groove and the glass cover, fixedly connected with rotates the base on the opposite side wall of separation axle and support base, be equipped with the spacing stop gear that is used for separating the axis rotation in-process on the lateral wall of rotation base, the cover is equipped with the detecting pipe on the lateral wall of rotation, the cover is equipped with the detecting instrument on the lateral wall of axis, detecting instrument and detecting pipe cooperation.

Preferably, the guide mechanism comprises a connecting ring fixedly connected to the side wall of the separation shaft, wherein the inner side wall of the connecting ring is fixedly connected with a plurality of circumferentially arranged guide plates, and the two guide plates are arranged on two sides of the sample injection hole.

Preferably, the mounting mechanism comprises a matching base fixedly connected to opposite side walls of the glass cover and the mounting groove, and a mounting block is fixedly connected to the side wall of the matching base.

Preferably, the limiting mechanism comprises a rotating ring fixedly connected to the side wall of the rotating base, which is close to one side of the separating shaft, a fixed pipe is rotationally connected in the rotating ring, a mounting ring is fixedly connected to the side wall of the fixed pipe, which is close to the inner top of the shell, and the mounting ring is fixedly connected with the inner top of the shell.

Preferably, two symmetrically arranged matching grooves are formed in the inner side wall of the mounting groove, and the matching grooves and the mounting block are arc-shaped and matched with each other.

Preferably, the mounting holes are formed in the side walls of the mounting ring and the mounting block, and the mounting ring and the mounting block are mounted in a matched manner through the pin rod and the mounting hole.

Compared with the prior art, the utility model has the beneficial effects that:

1. through setting up structures such as axis of rotation, release axle and sampling hole, utilize the axis of rotation to drive the release axle and rotate the centrifugation to the culture solution that is in it, rely on the effect of centrifugal force to realize the quick sample introduction of culture solution and can realize the evenly distributed sample introduction of culture solution in a plurality of sampling ports department simultaneously under the effect of centrifugal force.

2. Through setting up mounting groove, cooperation base and glass cover isotructure, utilize the circular-arc structure cooperation joint of installation piece and cooperation groove, the joint of cooperation base and mounting groove can realize the sealing to the department of sample inlet, and convenient opening simultaneously, when sealing sample inlet dust prevention, the separation condition that the accessible glass cover runs through the culture solution is convenient for observe the analysis.

Drawings

FIG. 1 is a schematic diagram showing the internal three-dimensional structure of a housing of a multi-well sample cell counter according to the present utility model;

FIG. 2 is a schematic diagram of the structure of a separation axis of the multi-well sample cell counter according to the present utility model;

fig. 3 is a schematic view of a glass cover structure of the porous sample cell counter according to the present utility model.

In the figure: 1 supporting base, 2 shell, 3 mounting groove, 4 collar, 5 fixed tube, 6 separation shaft, 7 sample injection hole, 8 connecting ring, 9 deflector, 10 rotating base, 11 shunt tubes, 12 detecting tube, 13 detector, 14 rotating shaft, 15 matching base, 16 glass cover, 17 installing block.

Detailed Description

In order that the above objects, features and advantages of the utility model will be readily understood, a more particular description of the utility model will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present utility model. The utility model may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit or scope of the utility model, which is therefore not limited to the specific embodiments disclosed below.

Referring to fig. 1-3, the multi-hole sample cell counter comprises a support base 1, the up end fixedly connected with shell 2 of support base 1, the rotation of shell 2 is connected with axis of rotation 14, the tip fixedly connected with separation axle 6 that support base 1 was kept away from to axis of rotation 14, a plurality of equidistant sample injection holes 7 that set up have been seted up on the lateral wall of separation axle 6, be equipped with the guiding mechanism who is used for assisting cell direction on the lateral wall of separation axle 6, guiding mechanism includes go-between 8 of fixed connection on separation axle 6 lateral wall, the inside wall fixedly connected with of go-between 8 a plurality of guide plates 9 that circumference set up, two guide plates 9 set up in the both sides of sample injection hole 7, wherein go-between 8 and guide plate 9 circulate the direction to the culture solution under centrifugal condition.

The shell 2 is kept away from and has offered mounting groove 3 on the one end lateral wall of supporting base 1, be equipped with glass cover 16 in the mounting groove 3, be equipped with the installation mechanism that is used for glass cover 16 installation between mounting groove 3 and the glass cover 16, installation mechanism includes the cooperation base 15 of fixed connection on the relative lateral wall of glass cover 16 and mounting groove 3, the lateral wall fixedly connected with installation piece 17 of cooperation base 15, two symmetries have been seted up to the inside wall of mounting groove 3 and have been set up the cooperation groove, the cooperation groove is circular-arc and mutually support with installation piece 17, the mounting hole has all been seted up on the lateral wall of collar 4 and installation piece 17, collar 4 and installation piece 17 pass through round pin pole and mounting hole cooperation installation.

The utility model provides a fixed connection has rotation base 10 on the relative lateral wall of release shaft 6 and support base 1, be equipped with on the lateral wall of rotation base 10 and be used for the spacing stop gear of release shaft 6 rotation in-process, stop gear includes fixed connection at the rotation ring that rotates on the lateral wall of base 10 and is close to release shaft 6 one side, rotate in the rotation ring and be connected with fixed pipe 5, fixed pipe 5 is fixed connection's on the lateral wall that is close to the top in shell 2 collar 4, the interior top of collar 4 fixed connection shell 2, wherein release shaft 6 is the circular cone structure for realize the reposition of redundant personnel of culture solution fast.

The lateral wall of rotating base 10 runs through the shunt tubes 11 of fixedly connected with a plurality of circumference settings, and the cover is equipped with detection tube 12 on the lateral wall of shunt tubes 11, and the cover is equipped with detector 13 on the lateral wall of axis of rotation 14, and detector 13 cooperates with detection tube 12.

When the device is used, as shown in fig. 1-3, firstly, the matching base 15 is clamped in the mounting groove 3 and matched with the arc structure of the mounting block 17 and the matching groove to mutually clamp, so that the stability of the glass cover 16 in the mounting process and the tightness between the matching base 15 and the mounting groove 3 can be assisted, the mounting of the matching pin rod and the mounting hole can be realized, the purposes of convenient mounting of the glass cover 16 and sealing of the sample inlet to prevent dust from entering are achieved, when the device is required to be used, firstly, the glass cover 16 is removed, the culture solution is poured onto the separation shaft 6, the cone structure of the separation shaft 6 is utilized to realize the diversion and circulation guiding of the culture solution, meanwhile, the rotating shaft 14 is driven to drive the separation shaft 6 to rotate at a slow speed, the culture solution can be driven to uniformly distribute at the positions of the sample inlets 7 under the action of centrifugal force, the culture solution is uniformly distributed in the plurality of the shunt pipes 11, the detection pipe 12 and the detection instrument 13 are matched, the fixed pipe 5 is fixedly connected onto the shell 2 through the mounting ring 4 in the process, the rotation base 10 is prevented from entering the separation shaft 9 in the sealing state, and the dust is prevented from entering the separation shaft 9 in the sealing state.

The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.

Claims (6)

1. The porous sample cell counter comprises a support base (1), and is characterized in that the upper end face of the support base (1) is fixedly connected with a shell (2), a rotating shaft (14) is rotationally connected with the shell (2), the rotating shaft (14) is far away from the end part of the support base (1) and is fixedly connected with a separation shaft (6), a plurality of equally spaced sample injection holes (7) are formed in the side wall of the separation shaft (6), a guide mechanism for assisting cell guiding is arranged on the side wall of the separation shaft (6), a mounting groove (3) is formed in the side wall of one end of the shell (2) far away from the support base (1), a glass cover (16) is arranged in the mounting groove (3) and the glass cover (16), a mounting mechanism for mounting the glass cover (16) is arranged between the mounting groove (3) and the glass cover, a rotating base (10) is fixedly connected with the opposite side wall of the support base (1), a limiting mechanism for limiting the rotation process of the separation shaft (6) is arranged on the side wall of the rotating base (10), a plurality of side walls (11) are fixedly connected with a plurality of shunt tubes (11) which are fixedly connected with the side walls (11) of the detection sleeve, the detector (13) is matched with the detection tube (12).

2. The multi-hole sample cell counter according to claim 1, wherein the guiding mechanism comprises a connecting ring (8) fixedly connected to the side wall of the separation shaft (6), the inner side wall of the connecting ring (8) is fixedly connected with a plurality of circumferentially arranged guiding plates (9), and two guiding plates (9) are arranged on two sides of the sample injection hole (7).

3. The multi-well sampling cytometer according to claim 2, wherein the mounting mechanism comprises a mating base (15) fixedly connected to opposite side walls of the glass cover (16) and the mounting groove (3), and a mounting block (17) is fixedly connected to a side wall of the mating base (15).

4. The multi-hole sample cell counter according to claim 3, wherein the limiting mechanism comprises a rotating ring fixedly connected to the side wall of the rotating base (10) close to the separating shaft (6), a fixed pipe (5) is rotatably connected to the rotating ring, a mounting ring (4) is fixedly connected to the side wall of the fixed pipe (5) close to the inner top of the housing (2), and the mounting ring (4) is fixedly connected to the inner top of the housing (2).

5. The multi-hole sample cell counter according to claim 4, wherein two symmetrically arranged matching grooves are formed in the inner side wall of the mounting groove (3), and the matching grooves and the mounting block (17) are arc-shaped and mutually matched.

6. The multi-hole sample cell counter according to claim 5, wherein the mounting holes are formed in the side walls of the mounting ring (4) and the mounting block (17), and the mounting ring (4) and the mounting block (17) are mounted in a matched manner through a pin rod and the mounting holes.

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