CN219016692U - Illumination system and microscope - Google Patents

Abstract

The utility model relates to an illumination system and a microscope. The lighting system comprises a standard light source, a lighting source, a light splitting element and a lens assembly, wherein the lens assembly is arranged on the light emitting side of the light splitting element, the light splitting element can receive at least part of light rays emitted by the standard light source and the lighting source, at least part of light rays emitted by the standard light source and the lighting source can be emitted towards the lens assembly, and the lens assembly is used for emitting light rays emitted by the light splitting element towards a sample. The lighting system has the advantages that the switching operation of the lighting source and the standard light source is simple, and the use is convenient.

Description

Illumination system and microscope

Technical Field

The utility model relates to the technical field of microscopic imaging, in particular to an illumination system and a microscope.

Background

At present, when a microstructure such as a biological tissue is inspected in the biomedical field, an image of the microstructure and spectral information of a specific wave band are generally required to be acquired so as to acquire the change of the microstructure in the aspects of content, structural morphology and the like. Wherein, the microscope is used to acquire the image of the microstructure, the illumination light source is used to illuminate the image, and the standard light source is used to perform spectrum calibration to acquire the spectrum information of the microstructure in a specific wave band. However, in the related art, the structure of the illumination light source and the standard light source of the microscope is complicated, and the use is inconvenient.

Disclosure of Invention

In view of this, it is necessary to provide an illumination system and a microscope, which solve the problems of complicated structure and inconvenience in use of an illumination light source and a standard light source of a microscope.

The utility model provides an illumination system, includes standard light source, illumination light source, beam split component and lens subassembly, the lens subassembly is located beam split component's light-emitting side, beam split component can receive standard light source with at least part light that illumination light source was emergent, and can with standard light source with at least part light that illumination light source was emergent is towards lens subassembly is emergent, lens subassembly is used for with the light that beam split component was emergent is towards the sample emergence.

In one embodiment, the light emitted by the standard light source and the light emitted by the illumination light source can be incident on the light splitting element from different sides of the light splitting element, and the light splitting element can reflect the light emitted by the standard light source and transmit the light emitted by the illumination light source.

In one embodiment, the light splitting element has a light splitting surface, the light splitting surface is inclined to the optical axis of the lens assembly, the light emitted by the illumination light source enters the light splitting element from a side of the light splitting surface facing away from the lens assembly, the light emitted by the standard light source enters the light splitting element from a side of the light splitting surface facing toward the lens assembly, and the light splitting surface can transmit the light emitted by the illumination light source and reflect the light emitted by the standard light source.

In one embodiment, the light splitting element includes a first right-angle prism and a second right-angle prism, the inclined surfaces of the first right-angle prism and the second right-angle prism are abutted to form the light splitting surface, one side surface of the first right-angle prism faces the lens assembly and is perpendicular to the optical axis of the lens assembly, the light emitted by the standard light source enters the light splitting element from the other side surface of the first right-angle prism, and the light emitted by the illumination light source enters the light splitting element from the side surface of the second right-angle prism.

In one embodiment, the incident direction of the light emitted by the standard light source and the incident direction of the light emitted by the illumination light source on the light splitting element are perpendicular to each other.

In one embodiment, the lens assembly includes a plurality of coaxially disposed convex lenses.

In one embodiment, the lighting system further includes a first controller, a light guiding element, and a light source interface, two ends of the light guiding element are respectively connected with the first controller and the light source interface, a light outlet of the light source interface is opposite to the light splitting element, and the first controller is used for coupling light emitted by the standard light source into the light guiding element.

In one embodiment, the illumination light source is fixed on a side of the light splitting element facing away from the lens assembly, and a light emitting surface of the illumination light source faces the light splitting element.

In one embodiment, the lighting system further comprises a second controller, the lighting source is provided with a positive electrode and a negative electrode, and the two electrodes of the second controller are respectively and electrically connected with the positive electrode and the negative electrode of the lighting source so as to control the on or off of the lighting source and/or control the output power of the lighting source.

A microscope comprising a stage for holding a sample and an illumination system according to any of the above embodiments for illuminating the sample.

According to the illumination system, the light emitted by the standard light source and the light emitted by the illumination light source can be transmitted to the sample by the matching of the light splitting element and the lens assembly to illuminate the sample, when the image of the sample needs to be acquired, the standard light source is only required to be turned off, and the illumination light source is turned on, so that the light emitted by the illumination light source can illuminate the sample through the light splitting element and the lens assembly, and similarly, when the spectrum information of the sample needs to be acquired, the standard light source is only required to be turned off and turned on. Therefore, in the lighting system, the lighting source and the standard light source are integrated in one system, the structure is simple, and the lighting source and the standard light source are not required to be detached, replaced or transferred when being switched for use, so that the lighting system is more convenient to use.

Drawings

Fig. 1 is a schematic diagram of a lighting system in some embodiments.

Reference numerals:

10. a lighting system; 101. a lighting module; 102. an illumination light source; 1022. a positive electrode; 1024. a negative electrode; 103. a spectroscopic element; 1032. a light splitting surface; 1034. a first right angle prism; 1036. a second right angle prism; 104. a lens assembly; 1042. a convex lens; 105. a first controller; 106. a light guide element; 107. a light source interface; 108. a second controller; 109. a control module; 201. and a stage.

Detailed Description

In order that the above objects, features and advantages of the utility model will be readily understood, a more particular description of the utility model will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present utility model. The present utility model may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the utility model, whereby the utility model is not limited to the specific embodiments disclosed below.

In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present utility model.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present utility model, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.

In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

In the present utility model, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.

It will be understood that when an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.

In the related art, a light source used for acquiring an image of a sample and spectrum information of the sample by a microscope is different, for example, an illumination light source is used for acquiring the image of the sample, and a standard light source is used for acquiring the spectrum information of the sample. In the related art, when the illumination light source and the standard light source are separately installed, and when the image of the sample and the spectrum information of the sample need to be obtained, the illumination light source and the standard light source are usually required to be switched, for example, one of the light sources is detached and the other light source is replaced, or the other light source is transferred to the sample through the transfer of a mechanical structure, so that the structure of the illumination system is complex, and the use is inconvenient.

In order to solve the above problems, the present application provides an illumination system and a microscope.

Referring to fig. 1, fig. 1 is a schematic diagram illustrating a lighting system 10 according to some embodiments. The illumination system 10 can be applied to a microscope, and the illumination system 10 can emit light to illuminate a sample of the microscope, so that the sample can be observed conveniently. Of course, the illumination system 10 may be used in any suitable device that requires illumination of a sample to facilitate observation of the sample, which may be a microstructure such as biological tissue. For example, the illumination system 10 may be applied to a microscope, where the sample is illuminated by emitting light in different wavelength bands to obtain an image or spectrum information of the sample, so as to observe the changes in content and structural morphology of the tissue component information caused by the disease of the biological tissue.

Further, in some embodiments, the illumination system 10 includes a standard light source (not shown), an illumination light source 102, a light splitting element 103, and a lens assembly 104, wherein the lens assembly 104 is disposed on the light emitting side of the light splitting element 103, and the light emitted from the standard light source and the illumination light source 102 can be incident on the light splitting element 103. The light-splitting element 103 can receive light emitted from the standard light source and the illumination light source 102, and can emit light emitted from the standard light source and the illumination light source 102 toward the lens assembly 104. The lens assembly 104 is used for emitting the light emitted by the light splitting element 103 towards the sample to illuminate the sample.

It will be appreciated that in the illumination system 10, the light splitting element 103 and the lens assembly 104 cooperate to transmit the light emitted from the standard light source and the illumination light source 102 to the sample for illuminating the sample. When the image of the sample needs to be acquired, only the standard light source is turned off, and the illumination light source 102 is turned on, so that the illumination light source 102 emits light, and the light emitted by the illumination light source 102 can illuminate the sample through the light splitting element 103 and the lens assembly 104. Similarly, when the spectrum information of the sample needs to be obtained, only the illumination light source 102 needs to be turned off and the standard light source needs to be turned on, and the light emitted by the standard light source can illuminate the sample through the light splitting element 103 and the lens assembly 104. Therefore, in the lighting system 10, the lighting source 102 and the standard light source are integrated in one system, the structure is simple, and when the lighting source 102 and the standard light source are switched, only the lighting source 102 and the standard light source are controlled to be turned on or off, the light source is not required to be detached, replaced or transferred, so that the lighting system 10 is more convenient to use.

It should be noted that, in the present application, it is described that a certain light source is turned on, which may be understood that the light source is turned on by controlling a switch button or a software circuit, or a light outlet of the light source is exposed by matching a mechanical structure, so that the light source can emit light, and when a certain light source is turned off, it may be understood that the light source is turned off by controlling the switch button or the software circuit, or the light outlet of the light source is blocked by matching the mechanical structure, so that the light source cannot emit light.

In the present application, the wavelength and type of the outgoing light of the illumination light source 102 are not limited as long as the illumination of the sample can be satisfied so as to obtain an image of the sample. For example, in some embodiments, the wavelength of the light emitted from the illumination source 102 is between 400nm and 1000nm, and the wavelength of the light emitted from the illumination source 102 covers a larger wavelength range, which is beneficial to more comprehensively acquiring the image of the sample and improving the accuracy of observing or detecting the sample. Specifically, the illumination light source 102 includes, but is not limited to, a light emitting element such as a light emitting diode, a halogen lamp, or the like. Of course, the wavelength and type of the light emitted from the illumination source 102 may be selected, and specifically may be designed according to the illumination requirement of the sample, so long as the illumination of the sample can be satisfied, and the wavelength range and type of the light emitted from the illumination source 102 are not specifically limited in this application.

It will be appreciated that when illumination of the sample is provided by the illumination source 102, the microscope may also be provided with a sensor which may be adapted to the range of wavelengths of light emitted by the illumination source 102, the sensor being arranged to receive light reflected from the sample to obtain an image of the sample.

In the application, the wavelength and type of the emergent light of the standard light source are not limited, so long as the spectrum calibration requirement on the sample can be met to obtain the spectrum information of the sample. For example, in some embodiments, the wavelength of the light emitted from the standard light source may be between 400nm and 1000nm, and of course, the wavelength of the light emitted from the standard light source may also have a larger range, for example, may be between 250nm and 1700nm, so as to obtain spectral information of a sample in a larger range, thereby improving the accuracy of detection. Standard light sources include, but are not limited to, light emitting elements such as mercury lamps, mercury argon lamps, xenon lamps, cadmium lamps, etc., and may be specifically designed according to the detected spectral range requirements.

It will be appreciated that when the standard light source is used to illuminate the sample, any suitable element or elements such as mercury (Hg), neon (Ne), argon (Ar), cadmium (Cd), cesium (Cs), helium (He), thallium (Tl) may be added to the sample, and the spectral information of the sample may be obtained by different reactions of the element or elements to light in different spectral ranges. When the standard light source is adopted to illuminate the sample, the microscope can be further provided with a device such as a spectrometer which can receive spectral information, and the spectrometer is used for receiving light generated by reflection or excitation of the sample so as to acquire the spectral information of the sample.

Further, in some embodiments, the light emitted from the standard light source and the illumination light source 102 can enter the light splitting element 103 from different sides of the light splitting element 103, and the light splitting element 103 can reflect at least part of the light emitted from the standard light source and transmit at least part of the light emitted from the illumination light source 102, so that at least part of the light emitted from the standard light source and the illumination light source 102 can be emitted toward the lens assembly 104.

For example, in some embodiments, the light splitting element 103 has a light splitting surface 1032, the light splitting surface 1032 is inclined to the optical axis of the lens assembly 104, the light emitted from the illumination light source 102 enters the light splitting element 103 from the side of the light splitting surface 1032 facing away from the lens assembly 104, the light emitted from the standard light source enters the light splitting element 103 from the side of the light splitting surface 1032 facing toward the lens assembly 104, and the light splitting surface 1032 is capable of transmitting the light emitted from the illumination light source 102 and reflecting the light emitted from the standard light source. The light emitted from the illumination light source 102 is transmitted through the light-splitting surface 1032 and then directed to the lens assembly 104, and the light emitted from the standard light source is reflected by the light-splitting surface 1032 toward the lens assembly 104. It should be understood that, in this application, taking the plane of the light splitting surface 1032 as an example, the plane divides the space on two sides, and the space on one side of the lens assembly 104, then the elements on the same side of the space as the lens assembly 104 may be considered to be located on the side of the light splitting surface 1032 facing the lens assembly 104, and the elements on the different side of the space as the lens assembly 104 may be considered to be located on the side of the light splitting surface 1032 facing away from the lens assembly 104.

In some embodiments, the light splitting element 103 includes a first right angle prism 1034 and a second right angle prism 1036, where the inclined surfaces of the first right angle prism 1034 abut to form the light splitting face 1032, e.g., where the first right angle prism 1034 is glued to the second right angle prism 1036. One side of the first right angle prism 1034 faces the lens assembly 104 and is perpendicular to the optical axis of the lens assembly 104, the other side is parallel to the optical axis of the lens assembly 104, and the light emitted from the standard light source enters the light splitting element 103 from the other side of the first right angle prism 1034. One side surface of the second right angle prism 1036 is perpendicular to the optical axis of the lens assembly 104, the other side surface is parallel to the optical axis of the lens assembly 104, and light emitted from the illumination light source 102 enters the spectroscopic element 103 from the side surface of the second right angle prism 1036 perpendicular to the optical axis of the lens assembly 104. In the present application, a surface of the right-angle prism corresponding to a hypotenuse of the right-angle triangle of the cross section is referred to as a slope of the right-angle prism, and two surfaces of the right-angle prism corresponding to two right-angle sides of the right-angle triangle of the cross section are referred to as sides of the right-angle prism.

The specific arrangement of the spectroscopic element 103 is not limited as long as it can reflect the light emitted from the standard light source and transmit the light emitted from the illumination light source 102, so that the light entering the spectroscopic element 103 from the different sides can be emitted toward the lens assembly 104. For example, in some embodiments, the light splitting element 103 is provided with a transflective film, and the transflective film is provided at the interface of the first right angle prism 1034 and the second right angle prism 1036, that is, at the light splitting surface 1032. The semi-transparent and semi-reflective film can transmit part of light rays emitted by the standard light source and reflect part of light rays emitted by the standard light source, and the semi-transparent and semi-reflective film can transmit part of light rays emitted by the illumination light source 102 and reflect part of light rays emitted by the illumination light source 102. For example, the semi-transparent and semi-reflective film can reflect 40%, 50% or 60% of the light emitted from the standard light source, and can transmit 40%, 50% or 60% of the light emitted from the illumination light source 102, so long as the standard light source or the illumination light source 102 emits light, and the illumination requirement of the sample can be satisfied. In other embodiments, the light splitting surface 1032 may further be provided with a selectively permeable film layer, and the material of the selectively permeable film layer may be designed according to the light emitted from the standard light source and the light emitted from the illumination light source 102, so as to reflect the light emitted from the standard light source to the greatest extent and transmit the light emitted from the illumination light source 102, for example, the light emitted from the standard light source can be reflected by more than 60%, and the light emitted from the illumination light source 102 can be transmitted by more than 60%, so as to improve the light utilization efficiency. The design relationship among the standard light source, the illumination light source 102 and the light splitting element 103, and the specific arrangement of the light splitting element 103 are not limited, for example, the light splitting element 103 may also be one or more light splitting prisms, as long as the light splitting element 103 can emit the light rays emitted from the standard light source and the illumination light source 102 incident from different positions toward the lens assembly 104, so as to illuminate the sample, thereby meeting the illumination requirement of the sample, and the arrangement of the light splitting element 103 is not specifically limited in the present application.

In the embodiment shown in fig. 1, the incident directions of the light rays emitted from the standard light source and the light rays emitted from the illumination light source 102 on the light splitting element 103 are perpendicular to each other, in other words, the central light ray of the light beam incident from the standard light source to the light splitting element 103 and the central light ray of the light beam incident from the illumination light source 102 to the light splitting element 103 are perpendicular to each other, and the light splitting surface 1032 can form an angle of 45 degrees with the optical axis of the lens assembly 104, so that the light rays emitted from the standard light source and the illumination light source 102 can be emitted toward the lens assembly 104 effectively, and the structural design and layout of the illumination system 10 are simpler. In other embodiments, the angle of the light splitting surface 1032 with respect to the optical axis of the lens assembly 104 and the incident angle of the light beams emitted from the standard light source and the illumination light source 102 on the light splitting element 103 may have other designs, as long as the light splitting element 103 can emit the light beams emitted from the standard light source and the illumination light source 102 toward the lens assembly 104, which is not repeated herein.

The lens assembly 104 can include the lens that the multichip has the focal power, and the cooperation of the lens that the multichip has the focal power makes the lens assembly 104 possess collimation and/or even light effect for the light that standard light source and illumination light source 102 are emergent can both be more even and throw light on the sample with good incident angle, promotes the illuminating effect, satisfies the demand of observing the sample. For example, the lens assembly 104 may include a plurality of convex lenses 1042, the plurality of convex lenses 1042 being coaxially disposed, and an axis common to the plurality of convex lenses 1042 may be understood as an optical axis of the lens assembly 104. Of course, the types and the number of the lenses in the lens assembly 104 may have other designs, and may be specifically designed according to the light emitting condition of the light splitting element 103 and the illumination requirement of the sample, which is not specifically limited in the present application.

In some embodiments, the lighting system 10 further includes a first controller 105, a light guiding element 106, and a light source interface 107, where the light guiding element 106 may be an optical fiber, and two ends of the light guiding element 106 are respectively connected to the first controller 105 and the light source interface 107, and the first controller 105 may be a controller of a standard light source, and the first controller 105 may be used to control on and off of the standard light source. The standard light source may be disposed in the first controller 105 or connected to the first controller 105, and the first controller 105 is further configured to couple light emitted by the standard light source into the light guiding element 106. The light source interface 107 may be abutted to the fixing structure of the light splitting element 103, and the light outlet of the light source interface 107 is opposite to the light splitting element 103, and the light emitted by the standard light source sequentially passes through the light guiding element 106 and the light source interface 107 and then is emitted toward the light splitting element 103.

In some embodiments, the illumination source 102 is fixed to a side of the light splitting element 103 facing away from the lens assembly 104, and a light emitting surface of the illumination source 102 faces the light splitting element 103. In some embodiments, the lighting system 10 further includes a second controller 108, where the second controller 108 may be a controller of the lighting source 102 for controlling the lighting source 102 to be turned on and off, and when the lighting source 102 is turned on, the second controller 108 may also control the output power of the lighting source 102 to meet different lighting requirements. For example, the illumination source 102 may have a positive electrode 1022 and a negative electrode 1024, and the two electrodes of the second controller 108 are electrically connected to the positive electrode 1022 and the negative electrode 1024 of the illumination source 102, respectively, to control the illumination source 102.

It should be understood that in the above embodiments, the illumination light source 102, the light splitting element 103 and the lens assembly 104 may be integrated into one illumination module 101, for example, the illumination light source 102, the light splitting element 103 and the lens assembly 104 are fixed to each other by a fixing structure, and the first controller 105 and the second controller 108 are disposed outside the illumination module 101, where the first controller 105 interfaces with the illumination module 101 through the light guiding element 106 and the light source interface 107, and the second controller 108 is electrically connected with the illumination light source 102 through wires. By the arrangement, the illumination system 10 has high structural integration, simple structural arrangement and wiring, high space utilization rate and convenient control operation of the illumination light source 102 and the standard light source.

Further, in some embodiments, the lighting system 10 may further include a control module 109, where the control module 109 may include an operation panel or a display panel, and the control module 109 may be electrically connected to the first controller 105 and the second controller 108, so as to control the first controller 105 and the second controller 108 by software or manually operating the control panel, and further control the lighting source 102 to be turned on and off with respect to the standard light source.

The present application also provides a microscope (not shown) comprising a stage 201 and an illumination system 10 according to any of the above embodiments, wherein the stage 201 is used for fixing a sample, and the light-emitting side of the lens assembly 104 may be opposite to the stage 201, so that the illumination system 10 illuminates the sample. In some embodiments, the microscope may further include other elements such as a spectrometer that may acquire spectral information of the sample when the standard light source is illuminated, a sensor that may acquire an image of the sample when the illumination light source 102 is illuminated, and the like.

Although not shown, it should be understood by those skilled in the art that the microscope and illumination system 10 of the present application may further include any other suitable elements to achieve the corresponding functions, and each of the above elements may be replaced by other elements to achieve the same functions, so long as the switching steps between the illumination light source 102 and the standard light source can be simplified, which is not described herein again.

The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.

The above examples illustrate only a few embodiments of the utility model, which are described in detail and are not to be construed as limiting the scope of the utility model. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the utility model, which are all within the scope of the utility model. Accordingly, the scope of protection of the present utility model is to be determined by the appended claims.

Claims (10)

1. The lighting system is characterized by comprising a standard light source, an illumination light source, a light splitting element and a lens assembly, wherein the lens assembly is arranged on the light emitting side of the light splitting element, the light splitting element can receive at least part of light rays emitted by the standard light source and the illumination light source, at least part of light rays emitted by the standard light source and the illumination light source can be emitted towards the lens assembly, and the lens assembly is used for emitting the light rays emitted by the light splitting element towards a sample.

2. A lighting system as recited in claim 1, wherein light from said standard light source and said lighting light source can be incident on said light-splitting element from different sides of said light-splitting element, and said light-splitting element can reflect light from said standard light source and transmit light from said lighting light source.

3. An illumination system as recited in claim 2, wherein said light splitting element has a light splitting surface which is inclined to the optical axis of said lens assembly, light rays exiting said illumination source are incident on said light splitting element from a side of said light splitting surface facing away from said lens assembly, light rays exiting said standard source are incident on said light splitting element from a side of said light splitting surface facing toward said lens assembly, and said light splitting surface is transparent to light rays emitted from said illumination source and reflects light rays emitted from said standard source.

4. A lighting system as recited in claim 3, wherein said light-splitting element comprises a first right-angle prism and a second right-angle prism, said first right-angle prism and said second right-angle prism having inclined surfaces which abut to form said light-splitting surface, one of said first right-angle prism side surfaces facing said lens assembly and being perpendicular to an optical axis of said lens assembly, light rays from said standard light source entering said light-splitting element from the other side surface of said first right-angle prism, and light rays from said lighting light source entering said light-splitting element from the side surface of said second right-angle prism.

5. A lighting system as recited in claim 2, wherein said light rays emitted from said standard light source and said light rays emitted from said lighting source are incident on said light-splitting element in directions perpendicular to each other.

6. The illumination system of claim 1, wherein the lens assembly comprises a plurality of coaxially disposed convex lenses.

7. A lighting system as recited in claim 1, further comprising a first controller, a light guide element, and a light source interface, wherein two ends of said light guide element are respectively connected to said first controller and said light source interface, a light outlet of said light source interface is opposite to said light splitting element, and said first controller is configured to couple light emitted from said standard light source into said light guide element.

8. A lighting system as recited in claim 1, wherein said illumination source is fixed to a side of said light-splitting element facing away from said lens assembly, and wherein a light-exiting surface of said illumination source faces said light-splitting element.

9. A lighting system as recited in claim 1, further comprising a second controller, said lighting source having a positive electrode and a negative electrode, said two electrodes of said second controller being electrically connected to said positive electrode and said negative electrode of said lighting source, respectively, to control said lighting source to be turned on or off and/or to control an output power of said lighting source.

10. A microscope comprising a stage for holding a sample and an illumination system according to any one of claims 1 to 9 for illuminating the sample.

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