CN218989273U

CN218989273U - Fermentation reaction system suitable for heterogeneous fermentation

Info

Publication number: CN218989273U
Application number: CN202223544707.8U
Authority: CN
Inventors: 刘振云; 杨松桦; 程丰伟
Original assignee: Suzhou Yixi Biotechnology Co ltd
Current assignee: Suzhou Yixi Biotechnology Co ltd
Priority date: 2022-12-28
Filing date: 2022-12-28
Publication date: 2023-05-09
Anticipated expiration: 2032-12-28

Abstract

The utility model relates to a fermentation reaction system suitable for multiphase fermentation. The fermentation reaction system comprises: the fermentation tank assembly comprises a fermentation tank body, a first monitoring assembly and a second monitoring assembly, wherein the first monitoring assembly is arranged at one axial end of the fermentation tank body, and the second monitoring assembly is arranged at one radial side of the fermentation tank body; the tank body rotating assembly comprises a rotating frame and a rotating driving mechanism, wherein the rotating frame is used for placing the fermentation tank assembly, and the rotating driving mechanism is used for driving the rotating frame to rotate so as to change the space position. The fermentation reaction system suitable for multiphase fermentation can be simultaneously suitable for solid fermentation and liquid fermentation, and can better meet actual production requirements.

Description

Fermentation reaction system suitable for heterogeneous fermentation

Technical Field

The utility model relates to the field of biological preparation, in particular to a fermentation reaction system suitable for multiphase fermentation.

Background

Fermentation is one of the core technologies of the biotechnology industry, and refers to a process of preparing microbial cells themselves or direct metabolites or secondary metabolites by means of the vital activities of microorganisms under aerobic or anaerobic conditions, which is a biochemical reaction performed under precisely controlled conditions. According to different physical properties of the reaction system, the method can be divided into solid fermentation and liquid fermentation; depending on the oxygen (air) demand, it can be classified into aerobic fermentation and anaerobic fermentation. Fermentation devices generally require the ability to precisely control reaction conditions, such as temperature, pH, dissolved oxygen, agitation, etc., while being able to record the values of various parameters during the reaction.

Existing fermentation devices are generally designed solely for solid or liquid fermentation and are generally in a fixed configuration, either vertical or horizontal. However, in the actual development and production process, there are cases of switching between solid fermentation and liquid fermentation, and the switching between the vertical fermentation tank and the horizontal fermentation is also required, and the above requirements cannot be satisfied by using the existing fermentation device.

Disclosure of Invention

Based on the above-mentioned drawbacks of the prior art, an object of the present utility model is to provide a fermentation reaction system suitable for multiphase fermentation, which is capable of changing the posture of a fermenter and can be applied to both solid fermentation and liquid fermentation.

Therefore, the utility model provides the following technical scheme.

The present utility model provides a fermentation reaction system suitable for multiphase fermentation, the fermentation reaction system comprising:

the fermentation tank assembly comprises a fermentation tank body, a first monitoring assembly and a second monitoring assembly, wherein the first monitoring assembly is arranged at one axial end of the fermentation tank body, and the second monitoring assembly is arranged at one radial side of the fermentation tank body;

the tank body rotating assembly comprises a rotating frame and a rotating driving mechanism, wherein the rotating frame is used for placing the fermentation tank assembly, and the rotating driving mechanism is used for driving the rotating frame to rotate so as to change the space position.

In at least one embodiment, the first monitoring assembly and the second monitoring assembly each include a temperature sensor, a PH electrode, a redox electrode, and a liquid take-off port.

In at least one embodiment, the fermenter assembly further comprises a condenser disposed at the axial end of the fermenter body;

the fermentation tank body is provided with a cooling water inlet and a cooling water outlet.

In at least one embodiment, the fermenter assembly further comprises a stirring mechanism comprising a stirring motor, a stirring shaft and a stirring paddle which are sequentially arranged along the axial direction of the fermenter body,

the stirring motor is arranged on the outer side of one axial end of the fermentation tank body, one end of the stirring shaft is connected with the stirring motor, the other end of the stirring shaft penetrates through the fermentation tank body and stretches into the fermentation tank body, and the stirring paddle is arranged on the stirring shaft and is positioned in the fermentation tank body; the stirring motor is used for driving the stirring shaft to rotate so as to drive the stirring paddle to rotate.

In at least one embodiment, the fermenter body further comprises a first feed supplement port and a second feed supplement port, the first feed supplement port is disposed at one axial end of the fermenter body, and the second feed supplement port is disposed at one radial side of the fermenter body.

In at least one embodiment, the fermenter assembly further comprises a tank support provided on the outside of the fermenter body for connecting the rotary frame;

the fermentation cylinder body is provided with the bin outlet, the bin outlet set up in the axial other end of fermentation cylinder body, bin outlet department is provided with the control valve in order to be used for controlling the break-make of bin outlet.

In at least one embodiment, the can rotation assembly further comprises a support frame, the rotation drive mechanism and the rotator being disposed on the support frame;

the rotary driving mechanism comprises a rotary motor and an output shaft which are connected in a transmission way, one end of the rotary frame is fixedly connected with the output shaft, the other end of the rotary frame is rotationally connected with the supporting frame, and the rotary motor is used for driving the output shaft to rotate so as to drive the rotary frame to synchronously rotate.

In at least one embodiment, the fermentation tank assembly further comprises a heating mechanism comprising a heating element disposed on the rotating frame for contact heating the fermentation tank assembly.

In at least one embodiment, further comprising a control assembly comprising a drive pump for pumping makeup liquid into the fermenter body;

wherein the control assembly is at least configured to replenish liquid into the fermentation tank body according to the monitoring result of the first monitoring assembly or the second monitoring assembly.

In at least one embodiment, the fermentation tank assembly and the tank rotating assembly are placed on a movable trolley, and the movable trolley moves with the fermentation tank assembly and the tank rotating assembly.

Advantageous effects

According to the fermentation reaction system suitable for multiphase fermentation, provided by the utility model, the first monitoring component and the second monitoring component are arranged, and the tank rotating component is arranged, so that the fermentation tank component can change the posture (for example, the horizontal posture and the vertical posture are switched), and further, the fermentation reaction system can be simultaneously suitable for solid fermentation and liquid fermentation, and the actual production requirements can be better met.

Drawings

Fig. 1 shows a schematic construction of a fermenter module according to the present utility model.

Fig. 2 shows a cross-sectional view of fig. 1.

Fig. 3 shows a schematic structural view of the can rotating assembly of the present utility model.

Fig. 4 shows a schematic structural diagram of the control assembly of the present utility model.

FIG. 5 shows a schematic diagram of the structure of a fermentation reaction system suitable for multi-phase fermentation of the present utility model.

Description of the reference numerals

1. A fermenter assembly;

11. a fermenter body; 111. a cooling water inlet; 112. a cooling water outlet; 113. a first feed supplement port; 114. a second feed supplement port; 115. a discharge port; 116. a control valve;

12. a first monitoring component; 121. a first temperature sensor; 122. a first pH electrode; 123. a first redox electrode; 124. a first liquid take-off;

13. a second monitoring component; 131. a second temperature sensor; 132. a second pH electrode; 133. a second redox electrode; 134. a second liquid take-off;

14. a condenser;

15. a stirring mechanism; 151. a stirring motor; 152. a stirring shaft; 153. stirring paddles;

16. a tank bracket;

2. a can body rotating assembly; 21. a rotating frame; 22. a rotary driving mechanism; 221. a rotating electric machine;

222. a speed reducer; 23. a support frame; 24. a heating mechanism; 241. a heating blanket support;

3. a control assembly; 31. driving a pump;

4. and (3) moving the trolley.

Detailed Description

In order to make the technical scheme and the beneficial effects of the utility model more obvious and understandable, the following detailed description is given by way of example. Unless defined otherwise, technical and scientific terms used herein have the same meaning as technical and scientific terms in the technical field to which this application belongs.

In the description of the present utility model, unless explicitly defined otherwise, terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "height", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., refer to an orientation or positional relationship based on that shown in the drawings, and are merely for convenience of simplifying the description of the present utility model, and do not indicate that the apparatus or element referred to must have a specific orientation, be constructed and operated in a specific orientation, i.e., are not to be construed as limiting the present utility model.

In the present utility model, the terms "first", "second" are used for descriptive purposes only and are not to be construed as relative importance of the features indicated or the number of technical features indicated. Thus, a feature defining "first", "second" may explicitly include at least one such feature. In the description of the present utility model, "plurality" means at least two; "plurality" means at least one; unless otherwise specifically defined.

In the present utility model, the terms "mounted," "connected," "secured," "disposed," and the like are to be construed broadly, unless otherwise specifically limited. For example, "connected" may be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, or can be communicated between two elements or the interaction relationship between the two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

In the present utility model, unless explicitly defined otherwise, a first feature "on", "above", "over" and "above", "below" or "under" a second feature may be that the first feature and the second feature are in direct contact, or that the first feature and the second feature are in indirect contact via an intermediary. Moreover, a first feature "above," "over" and "on" a second feature may be that the first feature is directly above or obliquely above the second feature, or simply indicates that the level of the first feature is higher than the level of the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the level of the first feature is less than the level of the second feature.

Specific embodiments of a fermentation reaction system suitable for multi-phase fermentation according to the present utility model are described in detail below with reference to FIGS. 1 to 5.

In the present embodiment, as shown in fig. 1 to 5, a fermentation reaction system suitable for multi-phase fermentation according to the present utility model includes a fermenter unit 1, a tank rotation unit 2, a control unit 3, and a traveling carriage 4.

As shown in fig. 1, the fermenter module 1 includes a fermenter body 11, a first monitor module 12, and a second monitor module 13. Wherein, first monitoring assembly 12 sets up in the axial one end of fermentation cylinder body 11, and second monitoring assembly 13 sets up in the radial one side of fermentation cylinder body 11.

As shown in fig. 2, the tank rotating assembly 2 includes a rotating frame 21 for receiving the fermenter assembly 1, and a rotation driving mechanism 22 for driving the rotating frame 21 to rotate to change a spatial position.

Through adopting above-mentioned technical scheme for the fermentation cylinder subassembly can change the gesture (for example, switches between horizontal and vertical gesture), and then can be applicable to solid fermentation and liquid fermentation simultaneously, satisfies actual production demand better.

In one embodiment, as shown in fig. 1, the first monitoring assembly 12 and the second monitoring assembly 13 have substantially the same structural composition, and the first monitoring assembly 12 includes a first temperature sensor 121, a first PH (Potential of hydrogen, hydrogen ion concentration index) electrode 122, a first redox electrode 123, and a first liquid take-off 124; the second monitoring assembly 13 includes a second temperature sensor 131, a second pH electrode 132, a second redox electrode 133, and a second liquid take-off 134. It will be appreciated that the temperature sensor is used to detect the temperature of the liquid in the fermenter, the pH electrode is used to detect the pH of the liquid in the fermenter, the redox electrode is used to detect the redox potential, and the liquid take-off is used to extract the liquid in the fermenter.

In one embodiment, as shown in fig. 1, the fermenter module 1 further includes a condenser 14, wherein the condenser 14 is disposed at one axial end of the fermenter module 11 and is located at the same end of the fermenter module 11 as the first monitor module 12. Further, the fermenter body 11 is provided with a cooling water inlet 111 and a cooling water outlet 112. Through the structure, the reaction temperature of the fermentation tank can be regulated and controlled. It should be understood that a separate condenser may be provided at the location of the second monitoring assembly 13, which is not limiting in this regard.

In one embodiment, as shown in fig. 1, the fermenter module 1 further includes a stirring mechanism 15, and the stirring mechanism 15 includes a stirring motor 151, a stirring shaft 152, and a stirring paddle 153 sequentially disposed in the axial direction of the fermenter body 11. Wherein, the stirring motor 151 is disposed at the outer side of one axial end of the fermentation tank body 11 and is located at the same end of the fermentation tank body 11 as the first monitoring component 12, one end of the stirring shaft 152 is connected with the stirring motor 151, the other end of the stirring shaft penetrates through the fermentation tank body 11 to extend into the fermentation tank body 11, and the stirring paddle 153 is disposed on the stirring shaft 152 and is located in the fermentation tank body 11; the stirring motor 151 is used for driving the stirring shaft 152 to rotate so as to drive the stirring paddle 153 to rotate. It will be appreciated that in carrying out the fermentation reaction, the liquid or fixed reactants in the fermenter body 11 can be stirred using the stirring mechanism 15, and the stirring speed for stirring the solid reactants is generally greater than the stirring speed for stirring the liquid reactants.

In an embodiment, as shown in fig. 1, a first feeding port 113 and a second feeding port 114 are disposed on the fermenter body 11, the first feeding port 113 is disposed at one axial end of the fermenter body 11 and is located at the same end of the fermenter body 11 as the first monitoring component 12, and the second feeding port 114 is disposed at one radial side of the fermenter body 11 and is located at the same radial side of the fermenter body 11 as the second monitoring component 13. It will be appreciated that the first feed port 113 and the second feed port 114 are used for feeding the fermenter body 11 in different postures.

In one embodiment, as shown in fig. 1, the fermenter module 1 further includes a tank support 16, and the tank support 16 is disposed on the outer side of the fermenter body 11 for supporting the fermenter body 11 and connecting the rotary frame 21.

In an embodiment, as shown in fig. 1 and 2, the fermenter body 11 is provided with a discharge port 115, the discharge port 115 is disposed at the other axial end of the fermenter body 11, and a control valve 116 is disposed at the discharge port 115 for controlling the on-off of the discharge port 115. Wherein the control valve 116 may be a butterfly valve or other structural form of control valve.

In one embodiment, as shown in fig. 3, the can rotating assembly 2 further includes a support frame 23, and the rotation driving mechanism 22 and the rotating frame 21 are disposed on the support frame 23. Further, the rotary driving mechanism 22 includes a rotary motor 221 and an output shaft (not shown in the figure) which are in transmission connection, one end of the rotary frame 21 is fixedly connected with the output shaft, the other end is rotatably connected with the supporting frame 23, and the rotary motor 221 is used for driving the output shaft to rotate, so as to drive the rotary frame 21 to synchronously rotate. Optionally, a decelerator 222 may be further provided between the rotary motor 221 and the rotary frame 21.

In one embodiment, as shown in fig. 3, the tank rotating assembly 2 further comprises a heating mechanism 24, and the heating mechanism 24 comprises a heating element, and the heating element is arranged on the rotating frame 21 and is used for heating the fermentation tank assembly 1 in a contact manner. Alternatively, the heating element may be a heating blanket, and the heating mechanism 24 may comprise a heating blanket holder 241, the heating blanket holder 241 being provided on the rotating frame 21, and the heating blanket may be provided on the heating blanket holder 241 for contacting the heating fermenter assembly 1. Wherein the blanket support 241 may be formed in a semi-circular arc shape to match the outer contour of the fermenter assembly 1.

In one embodiment, as shown in fig. 4, further comprising a control assembly 3, the control assembly 3 comprising a drive pump 31 for pumping makeup liquid into the fermenter body 11; the control assembly is configured to at least replenish the fermenter body 11 with liquid according to the monitoring result of the first monitoring assembly 12 or the second monitoring assembly 13. Wherein the drive pump 31 may be a peristaltic pump. Optionally, the control assembly 3 may further include related structures such as a drive pump switch, a pressure gauge, a flow meter, a display screen, a start button, a scram button, and a warning light. Alternatively, the control assembly 3 may be integrated on an electric cabinet.

In an embodiment, as shown in fig. 5, the fermenter assembly 1 and the tank rotating assembly 2 are moved to a predetermined position by the moving cart 4, and the moving cart 4 is used to place the fermenter assembly 1 and the tank rotating assembly 2.

Examples of production using the fermentation reaction system of the present utility model are described below.

In one embodiment, the aspergillus niger solid enzyme-producing fermentation is exemplified as follows: to a 5 liter volume fermenter body 11, 0.3kg of a fermented carbon source mixture (for example, bran: corn stalk: straw=3:2:1) was added, 2L of deionized water, 5g/L of ammonium sulfate, 7g/L of potassium dihydrogen phosphate, 5g/L of magnesium sulfate, and after sealing, fermenter assembly 1 was removed from rotary frame 21, and the whole was put into an autoclave for sterilization (set temperature: 121 ℃ C., 30 minutes). After cooling, the fermenter module 1 was mounted on a rotating frame 21, adjusted to a horizontal posture, inoculated with 30ml of Aspergillus niger liquid strain, inoculated with hydrochloric acid, naOH feed bottle, adjusted to control conditions, the temperature was controlled at 30 ℃, pH value was 4.3, stirring speed was 6 times/hour, ventilation volume was 100ml/min, and fermentation was carried out for 120 hours. Adding 2L of sterile deionized water, sealing, adjusting the vertical posture of the fermentation tank assembly 1, controlling the temperature to be 30 ℃, and reacting for 2 hours at the stirring speed of 300 rpm. The reaction mixture was transferred to a subsequent separation device through the bottom discharge port 115 for solid-liquid separation and extraction of crude enzyme liquid.

In one embodiment, taking yeast liquid fermentation to produce ethanol as an example, the following is specific: to the fermenter body 11 of the present utility model, 2L of yeast fermentation medium (for example, 15g/L of yeast extract, 20g/L of glucose, 5g/L of ammonium sulfate, 5g/L of magnesium sulfate, 2g/L of potassium dihydrogen phosphate, 10g/L of peptone) was added, and after sealing, the fermenter assembly 1 was removed from the rotary frame 21 and the whole was put into a sterilization pot for high-temperature sterilization (set temperature: 115 ℃ C., 30 minutes). After cooling, the fermenter module 1 was mounted on a rotary frame 21, the posture of the fermenter module 1 was adjusted to be vertical, a controller was adjusted, the fermenter temperature was controlled to 30 ℃, the pH was controlled to 5.0, the dissolved oxygen amount was controlled to 30%, the rotational speed was controlled to 200rpm, 100ml of Saccharomyces cerevisiae seed solution (OD (Optical Density) =8) was inoculated into the fermenter body 11, the pH was controlled using 29% ammonia water, and the feed was performed using a sterile glucose solution of 50% (w%). When the glucose concentration was reduced to 1g/L, glucose solution was added, and 800ml of glucose-supplemented solution was added within 24 hours of fermentation.

Further, 24 hours after fermentation starts, the introduced air is replaced by nitrogen, the oxidation-reduction potential of the reaction system is detected through an oxidation-reduction electrode, when the oxidation-reduction potential reaches 130mV, glucose solution dripping is started, after the anaerobic fermentation is continued for 72 hours, the reaction is stopped, the air outlet of the fermentation tank assembly 1 is communicated with a condensing device, the condensed water of the fermentation tank assembly 1 is stopped, the reactant in the fermentation tank is heated to 79-80 ℃, the stirring speed is 30rpm, in-situ ethanol distillation is carried out, and after the distillation is completed, the residual fermentation waste liquid is discharged from a lower end discharge port 115.

In one embodiment, the solid is fermented with liquid, and the yeast fermentation after degrading cellulose with Aspergillus niger is used for producing ethanol, and the method is as follows: to a 5 liter capacity fermenter body 11, 0.15kg of a fermentation carbon source mixture (for example, bran: corn stalk: straw=3:2:1) was added, 1L of deionized water was added, 5.0g/L of ammonium sulfate, 7.0g/L of monopotassium phosphate, and 5.0g/L of magnesium sulfate were added, and after sealing, fermenter assembly 1 was detached from rotary frame 21, and the whole was put into an autoclave for sterilization (set temperature: 121 ℃ C., 30 minutes). After cooling, the fermenter module 1 was mounted on a rotating frame 21, adjusted to a horizontal posture, inoculated with 15ml of Aspergillus niger liquid strain, inoculated with hydrochloric acid, naOH feed bottle, adjusted to control conditions, the temperature was controlled at 30 ℃, pH value was 4.5, stirring speed was 6 times/hour, ventilation volume was 100ml/min, and fermentation reaction was carried out for 240 hours.

Next, the temperature control was adjusted, the fermenter was heated to 100deg.C, the stirring speed was 30rpm, and the inactivation was performed in situ for 90 minutes.

Then, the condenser 14 was turned on, the reaction system was cooled to 30℃at 200rpm, 1.5L of yeast fermentation replenishment liquid (for example, 15g/L of yeast extract, 15g/L of ammonium sulfate, 5g/L of magnesium sulfate, 2g/L of monopotassium phosphate, 10g/L of peptone) was added to adjust the pH to 5.0, 500ml of Saccharomyces cerevisiae seed liquid (OD=8) was inoculated into the fermenter body 11, nitrogen gas was introduced for 1L/min, and the oxidation-reduction potential was detected for anaerobic fermentation for 72 hours. Terminating the reaction, namely connecting an air outlet of the fermentation tank assembly 1 with a condensing device, stopping condensing water of the fermentation tank assembly 1, heating reactants in the fermentation tank to 79-80 ℃, distilling in-situ ethanol at a stirring speed of 30rpm, and discharging residual fermentation waste liquid from a lower discharge port 115 after the distillation is completed.

In one embodiment, the fermentation is performed by liquid-solid connection, and the enzyme production is performed by liquid fermentation of aspergillus niger by solid fermentation, specifically as follows:

in a fermenter body 11 of the present utility model having a volume of 5 liters, 2L of deionized water was added, 20g/L of glucose was added, 5.0g/L of ammonium sulfate, 7.0g/L of potassium dihydrogen phosphate, and 5.0g/L of magnesium sulfate were added, and after sealing, the fermenter assembly 1 was removed from the rotary frame 21 and the whole was put into a high-pressure steam sterilizer for sterilization (set temperature: 115 ℃ C., 30 minutes). After cooling, the fermenter module 1 was mounted on a rotating frame 21, adjusted to a vertical posture, inoculated with 15ml of Aspergillus niger liquid strain, inoculated with hydrochloric acid, naOH feed supplement bottle, adjusted to control conditions, the temperature was controlled at 30 ℃, pH value was 4.5, stirring speed was 200rpm, aeration amount was 1000ml/min, and fermentation reaction was carried out for 72 hours.

After the reaction time, the fermenter module 1 was adjusted to a horizontal posture, 0.3kg of a fermented carbon source mixture (for example, bran: corn stalk: straw=3:2:1) was added, 5.0g/L of ammonium sulfate, 7.0g/L of monopotassium phosphate, 5.0g/L of magnesium sulfate, control conditions were adjusted, the control temperature was 30 ℃, pH was 4.3, the stirring speed was 6 times/hour, the aeration rate was 100ml/min, and the fermentation was performed for 120 hours. Adding 2L of sterile deionized water, sealing, adjusting the vertical posture of the fermentation tank assembly 1, controlling the temperature to be 30 ℃, and reacting for 2 hours at the stirring speed of 300 rpm. The reaction mixture was transferred to a subsequent separation device through the bottom discharge port 115 for solid-liquid separation and extraction of crude enzyme liquid.

It should be understood that the above embodiments are exemplary and not intended to encompass all possible embodiments encompassed by the claims. Various modifications and changes may be made in the above embodiments without departing from the scope of the utility model. Likewise, the individual technical features of the above embodiments may also be combined arbitrarily to form further embodiments of the utility model which may not be explicitly described. Therefore, the above embodiments merely represent several embodiments of the present utility model, and do not limit the scope of the present utility model.

Claims

1. A fermentation reaction system suitable for use in multiphase fermentation, the fermentation reaction system comprising:

2. The fermentation reaction system of claim 1, wherein the first monitoring assembly and the second monitoring assembly each comprise a temperature sensor, a PH electrode, a redox electrode, and a liquid take-off.

3. The fermentation reaction system of claim 1, wherein the fermenter assembly further comprises a condenser disposed at the axial end of the fermenter body;

4. The fermentation reaction system of claim 1, wherein the fermenter module further comprises a stirring mechanism comprising a stirring motor, a stirring shaft and a stirring paddle sequentially arranged along the axial direction of the fermenter body,

5. The fermentation reaction system of claim 1, wherein the fermenter body further comprises a first feed supplement port and a second feed supplement port, the first feed supplement port being disposed at the axial end of the fermenter body, the second feed supplement port being disposed on a radial side of the fermenter body.

6. The fermentation reaction system of claim 1, wherein the fermenter assembly further comprises a tank mount disposed outside of the fermenter body for connecting the rotary mount;

7. The fermentation reaction system of claim 1, wherein the tank rotation assembly further comprises a support frame, the rotation drive mechanism and the rotator being disposed on the support frame;

8. The fermentation reaction system of claim 7, further comprising a heating mechanism comprising a heating element disposed on the rotating frame for contact heating the fermenter assembly.

9. The fermentation reaction system of claim 1, further comprising a control assembly including a drive pump for pumping makeup liquid into the fermenter body;

10. The fermentation reaction system of claim 1, further comprising a mobile cart for positioning and moving the fermenter assembly and the tank rotating assembly.