CN218969256U - Bioreactor heating device and wave-type bioreactor - Google Patents

Abstract

The utility model provides a bioreactor heating device and a wave-type bioreactor, which relate to the technical field of bioreactors, and the bioreactor heating device comprises: the device comprises a cradle, a plurality of heating blankets and a plurality of temperature sensors; the bearing area of the cradle is provided with a plurality of cell culture stations, and each cell culture station is provided with a heating blanket and a temperature sensor. According to the bioreactor heating device provided by the utility model, a single large-capacity cell culture bag can be jointly borne by the plurality of cell culture stations, small-capacity cell culture bags can also be respectively borne by the plurality of cell culture stations, the plurality of cell culture stations can respectively regulate and control the temperature, and the control precision of the cell culture temperature is improved.

Description

Bioreactor heating device and wave-type bioreactor

Technical Field

The utility model relates to the technical field of bioreactors, in particular to a bioreactor heating device and a wave-type bioreactor.

Background

The wave bioreactor adopts non-intervening wave shaking to provide shaking motion power for the culture solution, so that the culture solution generates waves, and gas is contacted with the cell culture to meet the growth requirement of the cell culture. In this process, the culture temperature needs to be monitored and controlled, and if a plurality of small-capacity cell culture bags are used, it is difficult to accurately control the culture solution inside each cell culture bag. In addition, whether the temperature detection function is abnormal or not cannot be known, the temperature detection failure cannot be timely handled, and the risk of exceeding the culture temperature exists. In addition, when the temperature is low, the cell culture liquid is usually required to be heated, and the temperature detection result cannot reflect the actual temperature of the cell culture liquid because the heat of the heating device is difficult to avoid being directly transmitted to the temperature sensor, so that the control accuracy of the cell culture temperature is affected.

Disclosure of Invention

The utility model aims to provide a bioreactor heating device and a wave-type bioreactor so as to relieve the technical problem of low control precision of cell culture temperature.

In a first aspect, the present utility model provides a bioreactor heating device comprising: the device comprises a cradle, a plurality of heating blankets and a plurality of temperature sensors;

the bearing area of the cradle is provided with a plurality of cell culture stations, and each cell culture station is provided with the heating blanket and the temperature sensor.

With reference to the first aspect, the present utility model provides a first possible implementation manner of the first aspect, wherein a plurality of the cell culture stations are respectively provided with a mounting groove, and a plurality of the heating blankets are mounted in a one-to-one correspondence manner in the plurality of mounting grooves.

With reference to the first aspect, the present utility model provides a second possible implementation manner of the first aspect, wherein the cradle is provided with a wiring groove, and the wiring of the heating blanket passes through the wiring groove.

With reference to the first aspect, the present utility model provides a third possible implementation manner of the first aspect, wherein a through hole is provided in a middle portion of the heating blanket, and the temperature sensor is installed in the through hole.

With reference to the third possible implementation manner of the first aspect, the present utility model provides a fourth possible implementation manner of the first aspect, wherein the heating blanket is provided with a yielding area without a heating element, and the yielding area surrounds the through hole.

With reference to the fourth possible implementation manner of the first aspect, the present utility model provides a fifth possible implementation manner of the first aspect, wherein a difference between a radius of the relief area and a radius of the through hole is 4mm to 10mm.

With reference to the first aspect, the present utility model provides a sixth possible implementation manner of the first aspect, wherein the cradle is provided with a sensor slot, and the temperature sensor passes through the heating blanket and is inserted into the sensor slot.

With reference to the first aspect, the present utility model provides a seventh possible implementation manner of the first aspect, wherein the cradle is provided with a routing hole, and the wiring of the temperature sensor passes through the routing hole.

With reference to the first aspect, the present utility model provides an eighth possible implementation manner of the first aspect, wherein the heating blanket includes: a heating layer and a heat insulating layer;

the heating layer is attached to the heat insulation layer, and the heat insulation layer is located between the heating layer and the shaking table.

In a second aspect, the utility model provides a wave bioreactor comprising any one of the bioreactor heating devices of the first aspect.

The embodiment of the utility model has the following beneficial effects: the bearing area of the shaking table is provided with a plurality of cell culture stations, each cell culture station is provided with a heating blanket and a temperature sensor, a single large-capacity cell culture bag can be jointly borne by the plurality of cell culture stations, small-capacity cell culture bags can also be respectively borne by the plurality of cell culture stations, the plurality of cell culture stations can respectively regulate and control the temperature, and the control precision of the cell culture temperature is improved.

In order to make the above objects, features and advantages of the present utility model more comprehensible, preferred embodiments accompanied with figures are described in detail below.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the related art, the drawings that are required to be used in the description of the embodiments or the related art will be briefly described, and it is apparent that the drawings in the description below are some embodiments of the present utility model, and other drawings may be obtained according to the drawings without inventive effort for those skilled in the art.

FIG. 1 is a schematic view of a bioreactor heating device according to an embodiment of the present utility model;

FIG. 2 is a schematic view of a heating blanket of a bioreactor heating device according to an embodiment of the present utility model;

fig. 3 is a schematic diagram of a shaker of a bioreactor heating device according to an embodiment of the present utility model.

Icon: 100-shaking table; 101-a cell culture station; 102-a mounting groove; 103-wiring grooves; 104-a sensor slot; 105-wiring holes; 200-heating the blanket; 201-a through hole; 210-a heat generating layer; 220-insulating layer; 300-temperature sensor.

Detailed Description

The following description of the embodiments of the present utility model will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the utility model are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Physical quantities in the formulas, unless otherwise noted, are understood to be basic quantities of basic units of the international system of units, or derived quantities derived from the basic quantities by mathematical operations such as multiplication, division, differentiation, or integration.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

As shown in fig. 1, a bioreactor heating device provided in an embodiment of the present utility model includes: shaker 100, plurality of heating blankets 200, and plurality of temperature sensors 300; the carrying area of the shaker 100 has a plurality of cell culture stations 101, each cell culture station 101 being fitted with a heating blanket 200 and a temperature sensor 300.

When in use, the cell culture bags with small capacity can be respectively loaded through the plurality of cell culture stations 101, and the cell culture bags with large capacity can also be jointly loaded through the plurality of cell culture stations 101. Each cell culture station 101 is provided with a heating blanket 200 and a temperature sensor 300, so that heating and temperature detection can be performed respectively, further, temperature control of each cell culture station 101 is realized, and control accuracy of cell culture temperature is improved.

As shown in fig. 1 and 3, in the embodiment of the present utility model, a plurality of cell culturing stations 101 are respectively provided with a plurality of mounting grooves 102, and a plurality of heating blankets 200 are mounted in the plurality of mounting grooves 102 in a one-to-one correspondence.

Specifically, the heating blanket 200 is clamped in the mounting groove 102, so as to ensure the smoothness of the bearing surface of the cell culture station 101. When a cell culture bag is placed in the cell culture station 101 and swings with the swing table 100, the heating blanket 200 is limited in the mounting groove 102, so that the heating blanket 200 is prevented from moving relative to the swing table 100.

Further, the cradle 100 is provided with a wiring groove 103, and wiring of the heating blanket 200 passes through the wiring groove 103.

Specifically, each cell culturing station 101 is provided with a wiring groove 103, the wiring groove 103 is located at the bottom of the mounting groove 102, and the wiring of the heating blanket 200 can extend outwards through the wiring groove 103, so that not only can the flatness of the heating blanket 200 be ensured, but also the damage of the wiring of the heating blanket 200 caused by compaction and bending can be prevented.

As shown in fig. 1, 2 and 3, a through hole 201 is provided at the middle of the heating blanket 200, and a temperature sensor 300 is installed in the through hole 201. The heating blanket 200 and the temperature sensor 300 may be attached to the bottom of the cell culture bag, the culture medium inside the cell culture bag may be heated by the heating blanket 200, and the temperature of the culture medium may be detected by the temperature sensor 300 contacting the cell culture bag. When the detected temperature is lower than the set temperature, the controller controls the heating blanket 200 to start heating; when the detected temperature is equal to or higher than the set temperature, the controller controls the heating blanket 200 to stop heating, thereby ensuring that the temperature of the culture solution meets the set temperature. In the case that the plurality of cell culture stations 101 together bear a cell culture bag with a larger capacity, if the difference between the test values of two adjacent temperature sensors 300 exceeds the set deviation, it can be determined that the temperature sensor 300 has a fault, and at this time, the reactor can be controlled to send an alarm to prompt personnel to overhaul in time.

Further, the heating blanket 200 is provided with a relief area without heating elements, and the relief area surrounds the through hole 201.

Specifically, the relief area is configured as an annular area surrounding the through hole 201, and no heating element is arranged in the annular area, so that heat emitted by the heating blanket 200 is prevented from being transferred to the temperature sensor 300 installed in the through hole 201, and the accuracy of temperature detection of the temperature sensor 300 is ensured.

Further, the difference between the radius of the relief area and the radius of the through hole 201 is 4 mm-10 mm, so that the relief area has a sufficient width to prevent the heat of the heating blanket 200 from being transferred to the temperature sensor 300.

Further, the cradle 100 is provided with sensor slots 104, and the temperature sensor 300 is inserted through the heating blanket 200 and is inserted into the sensor slots 104.

Specifically, the temperature sensor 300 passes through the through hole 201 and is inserted into the sensor slot 104, so that the temperature sensor 300 can be clamped or screwed in the sensor slot 104 to ensure stability of the temperature sensor 300.

In addition, the cradle 100 is provided with a wiring hole 105, and wiring of the temperature sensor 300 passes through the wiring hole 105. The wiring hole 105 is located at the bottom of the sensor slot 104, and the wiring of the temperature sensor 300 is led out through the wiring hole 105, so as to avoid the damage caused by bending or extrusion of the wiring of the temperature sensor 300.

As shown in fig. 1 and 2, the heating blanket 200 includes: a heat generating layer 210 and a heat insulating layer 220; the heating layer 210 is attached to the heat insulating layer 220, and the heat insulating layer 220 is located between the heating layer 210 and the cradle 100.

Specifically, the heating element is disposed inside the heating layer 210, the heating element can adopt an electric heating wire or an electric heating belt, the heat insulation layer 220 adopts a material with low thermal conductivity, and the heat insulation layer 220 is attached to the bottom of the heating layer 210, and is isolated between the heating layer 210 and the shaking table 100 through the heat insulation layer 220, so that the heating element has a heat preservation effect, and can prevent heat of the heating layer 210 from being transferred to the shaking table 100, and avoid scalding the surface of the shaking table 100.

The wave-type bioreactor provided by the embodiment of the utility model comprises the bioreactor heating device in any embodiment.

In the embodiment of the present utility model, the wave-type bioreactor is equipped with the bioreactor heating device, so that the bearing surface of the cell culture station 101 can be configured into an arc-shaped curved surface, the axis of the arc-shaped curved surface is parallel to the swing axis of the swing table 100, and the heating blanket 200 is arranged along the arc-shaped curved surface, so that the cell culture bag can be ensured to be closely attached to the heating blanket 200. The wave-type bioreactor has the technical effects of the bioreactor heating device, and is not described herein.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model, and not for limiting the same; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the utility model.

Claims (10)

1. A bioreactor heating apparatus, comprising: a cradle (100), a plurality of heating blankets (200), and a plurality of temperature sensors (300);

the bearing area of the cradle (100) is provided with a plurality of cell culture stations (101), and each cell culture station (101) is provided with the heating blanket (200) and the temperature sensor (300).

2. Bioreactor heating device according to claim 1, characterized in that a plurality of the cell culture stations (101) are respectively provided with mounting grooves (102), and a plurality of the heating blankets (200) are mounted in the plurality of the mounting grooves (102) in a one-to-one correspondence.

3. Bioreactor heating device according to claim 1 or 2, characterized in that the rocking bed (100) is provided with wiring grooves (103), the wiring of the heating blanket (200) passing through the wiring grooves (103).

4. Bioreactor heating device according to claim 1, characterized in that the middle part of the heating blanket (200) is provided with a through hole (201), and the temperature sensor (300) is installed in the through hole (201).

5. Bioreactor heating device according to claim 4, characterized in that the heating blanket (200) is provided with a yielding zone without heating elements, which yielding zone surrounds the through hole (201).

6. Bioreactor heating device according to claim 5, characterized in that the radius of the relief zone differs from the radius of the through hole (201) by 4-10 mm.

7. Bioreactor heating device according to claim 1, characterized in that the shaking table (100) is provided with sensor slots (104), and the temperature sensor (300) is inserted through the heating blanket (200) and into the sensor slots (104).

8. Bioreactor heating device according to claim 1, characterized in that the rocking bed (100) is provided with wiring holes (105), the wiring of the temperature sensor (300) passing through the wiring holes (105).

9. Bioreactor heating device according to claim 1, characterized in that the heating blanket (200) comprises: a heat generating layer (210) and a heat insulating layer (220);

the heating layer (210) is attached to the heat insulation layer (220), and the heat insulation layer (220) is located between the heating layer (210) and the shaking table (100).

10. A wave bioreactor, characterized in that it comprises a bioreactor heating device according to any one of claims 1-9.

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