CN218924656U

CN218924656U - CPG module with 1 fmol-1 nmol synthesis yield

Info

Publication number: CN218924656U
Application number: CN202223529615.2U
Authority: CN
Inventors: 张会刚; 郎秋蕾; 李璐璐; 肖晔
Original assignee: Hangzhou Lianchuan Biotechnology Co ltd
Current assignee: Hangzhou Lianchuan Biotechnology Co ltd
Priority date: 2022-12-29
Filing date: 2022-12-29
Publication date: 2023-04-28
Anticipated expiration: 2032-12-29

Abstract

A CPG module with 1 fmol-1 nmol synthesis yield belongs to the technical field of DNA recombination, and comprises a CPG carrier plate, wherein the lower end surface of the CPG carrier plate is connected with a liquid separation plate through bolt locking; the CPG carrier plate comprises a synthesis hole which is formed in the CPG carrier plate, CPG columns are sintered in the synthesis hole, liquid discharge channels are formed in the liquid separation plate, and the synthesis hole corresponds to the liquid discharge channels one by one; the CPG column is formed by premixing CPG powder and ultra-high molecular weight polyethylene powder, sieving the mixture into a synthetic hole and sintering the mixture, wherein the lower end of the CPG column is flush with the lower end surface of the CPG carrier plate; firstly, through the expansion and the maintenance of later stage of modularization be convenient for, and need not to consider because certain part damages the economic loss that changes whole device and bring, in addition, adopts the sintering of CPG post in the synthetic downthehole, avoid CPG post installation accuracy difference big, lead to the big problem of synthetic gas resistance difference, promote synthetic efficiency and synthetic quality.

Description

CPG module with 1 fmol-1 nmol synthesis yield

Technical Field

The utility model belongs to the technical field of DNA recombination, and particularly relates to a CPG module with a 1 fmol-1 nmol synthesis yield.

Background

The DNA synthesis yield depends on the molar concentration of CPG, and the air resistance of CPG column is an important index, directly affecting the quality of synthesis. The air resistance affecting CPG is the molar concentration and uniformity of the filled CPG. CPG, which is common in the market, is mostly a mode of loading a CPG carrier into a synthesis module after sintering, and is suitable at the time of low flux.

However, at the synthesis flux of 1K-10K and above (high flux for short), there is a serious problem that CPG installation accuracy difference is large due to small CPG volume of high flux synthesis flux, CPG air resistance can be affected, air resistance difference is increased, and finally synthesis efficiency and quality are affected.

Disclosure of Invention

The utility model aims to provide a CPG module with a synthesis yield of 1 fmol-1 nmol, so as to solve the problems in the background technology.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

the CPG module with the 1 fmol-1 nmol synthesis yield comprises a CPG carrier plate, wherein the lower end surface of the CPG carrier plate is connected with a liquid separation plate through bolt locking; the CPG carrier plate comprises a synthesis hole which is formed in the CPG carrier plate, CPG columns are sintered in the synthesis hole, liquid discharge channels are formed in the liquid separation plate, and the synthesis hole corresponds to the liquid discharge channels one by one; the CPG column is formed by premixing CPG powder and ultra-high molecular weight polyethylene powder, sieving the mixture into a synthetic hole and sintering the mixture, wherein the lower end of the CPG column is flush with the lower end face of the CPG carrier plate.

Compared with the prior art, the technical scheme has the following effects:

firstly, through the expansion and the maintenance of later stage of modularization be convenient for, and need not to consider because certain part damages the economic loss that changes whole device and bring, in addition, adopts the sintering of CPG post in the synthetic downthehole, avoid CPG post installation accuracy difference big, lead to the big problem of synthetic gas resistance difference, promote synthetic efficiency and synthetic quality.

Preferably, the synthesis hole comprises a liquid inlet section, a transition section and a CPG carrier section which are sequentially arranged from top to bottom, the liquid inlet section, the transition section and the CPG carrier section are sequentially connected, and the CPG column is sintered in the CPG carrier section.

Preferably, the CPG powder comprises a mixture of non-silanized CPG powder and silanized CPG powder.

Preferably, the inner diameter of the liquid inlet section is larger than that of the CPG carrier section, and the cross section of the transition section in the vertical direction is in an inverted trapezoid shape.

Preferably, the CPG carrier plate is made of a high-density PP material.

Preferably, the inner surface of the synthetic aperture is sprayed with a teflon coating.

Preferably, the liquid separation plate comprises a liquid separation plate body, a liquid separation cavity is formed in the upper end face of the liquid separation plate body, liquid discharge channels are arranged on the lower end wall of the liquid separation cavity in an array mode, bosses are arranged on the lower end face of the liquid separation plate body, and the lower ends of the liquid discharge channels penetrate through the lower end faces of the bosses.

Preferably, a filter membrane is arranged between the liquid separation plate and the CPG carrier plate, and the filter membrane is sintered on the lower surface of the CPG carrier plate or the upper surface of the liquid separation plate in an assembled state; or, the lower surface of the filter membrane is pressed on the upper surface of the liquid separation plate, and the upper surface of the filter membrane is pressed on the lower surface of the CPG carrier plate.

Preferably, the CPG carrier plate comprises a carrier plate body, and a threaded hole for accommodating a bolt and a waist-shaped groove staggered with the threaded hole are formed in the edge of the upper end face of the carrier plate body.

Preferably, a concave cavity is concavely arranged in the middle of the upper end face of the carrier plate body, and the synthesis hole array is arranged in the concave cavity.

Drawings

FIG. 1 is a schematic view of the overall structure of the present utility model;

FIG. 2 is a schematic view of the overall structure in the "A" direction in FIG. 1;

FIG. 3 is an exploded schematic view of the overall structure of the present utility model;

FIG. 4 is a schematic view of the overall structure in the "B" direction in FIG. 3;

fig. 5 is a schematic cross-sectional view of the present utility model.

In the figure: 1. CPG carrier plate; 10. a carrier plate body; 11. synthesizing a hole; 110. a liquid inlet section; 111. a transition section; 112. CPG carrier segment; a. CPG column; 12. a threaded hole; 13. a kidney-shaped groove; 14. a cavity; 2. CPG divides the liquid board; 20. a liquid separation plate body; 200. a connection hole; 21. a boss; 22. a liquid discharge channel; 23. a liquid separating cavity; 3. a bolt; 4. and (5) a filter membrane.

Detailed Description

The following description of the embodiments of the present utility model will be made with reference to the accompanying drawings, in which it is evident that the embodiments described are only some embodiments of the present utility model, but not all embodiments.

Examples:

the CPG in this embodiment has the following meanings: controlled pore glass, controllable microporous glass beads.

The CPG module with the synthesis yield of 1 fmol-1 nmol shown in the figures 3-4 comprises a CPG carrier plate 1, a filter membrane 4 and a liquid separation plate 2 which are sequentially arranged from top to bottom;

in the combined state, as shown in fig. 1-2, the CPG carrier plate 1 includes a carrier plate body 10, a concave cavity 14 is formed by recessing an upper end surface of the carrier plate body 10 inward, a plurality of synthetic holes 11 are disposed on an array of a lower end surface of the concave cavity 14, a plurality of threaded holes 12 are disposed on an edge of an upper end surface of the carrier plate body 10, bolts 3 are disposed in the plurality of threaded holes 12, a lower end of each bolt 3 is connected to the corresponding liquid separation plate 2, that is, a connecting hole 200 is disposed at a position of the liquid separation plate 2 for connecting the bolts 3, and referring to fig. 5, the threaded holes 12 and the connecting holes 200 are not literally the same, that is, the carrier plate body 10 must be disposed with the threaded holes 12, and the liquid separation plate 2 is disposed with the connecting holes 200 only for better distinction and precise expression.

Furthermore, the upper end surface edge of the carrier plate body 10 is provided with a kidney-shaped groove 13, the kidney-shaped groove 13 and the threaded holes 12 are staggered, and the following is provided by arranging the kidney-shaped groove 13: 1. the CPG carrier plate 1 in the embodiment is prepared by adopting an injection molding process, so that the technical requirement of uniform wall thickness of the injection molding process is met; 2. the groove walls of the kidney-shaped grooves 13 form rib plates of the carrier plate, so that the structural strength of the carrier plate is enhanced.

It should be noted that, in order to achieve better reaction and subsequent cleaning of the reagent in the synthesis hole 11, the synthesis hole 11 sequentially includes a liquid inlet section 110, a transition section 111 and a CPG carrier section 112 from top to bottom, and the three sections are sequentially connected, that is, the lower end of the liquid inlet section 110 is connected to the upper end 111 of the transition section 111, and the lower end of the transition section 111 is connected to the upper end of the CPG carrier section 112, in this embodiment, the liquid inlet section 110, the transition section 111 and the CPG carrier section 112 are integrally formed, and in an ideal state, the inner surfaces formed by the three sections should be arc surfaces, but based on the technical difficulty in a real scene, the following scheme is adopted: the inner diameter of the liquid inlet section 110 is larger than the inner diameter of the CPG carrier section 112, and the inner side surface of the transition section 111 is an inclined plane; the transition section 111 is of an inclined surface structure, injection molding is convenient, the die stripping is convenient, the size is stable, meanwhile, the liquid inlet section 110 and the CPG carrier section 112 are communicated, so that a buffering effect is achieved, and secondly, the surface is smooth, dead angles are avoided, and reagent flowing and reagent cleaning are facilitated.

Next, the CPG column a is disposed in the CPG carrier section 112, and referring to fig. 3 and 4, the CPG column a is shown only to be disposed in the synthetic hole 11, and the positional relationship thereof corresponding to the liquid separation plate 2 will be described again, so that the description will not be focused herein, but it should be noted that the CPG column a employed in the present embodiment is not prepared in advance, but is prepared in advance by a sintering process, and the specific preparation process is as follows:

(1) Preparation of raw materials: in the embodiment, CPG powder with 2000 pores and ultra-high molecular weight polyethylene are selected as raw materials, wherein the CPG powder is non-silanized CPG powder (called blank CPG powder for short);

(2) The preparation method comprises the following steps: and (3) carrying out silanization treatment on part of blank CPG powder in CPG powder to obtain silanized CPG powder, premixing the blank CPG powder and the silanized CPG powder according to the actually required CPG molar concentration, premixing the blank CPG powder and the silanized CPG powder with ultra-high molecular weight polyethylene to obtain mixed powder, screening the mixed powder into a CPG carrier section 112 through a vibrating screen, and preparing a CPG column a through a sintering process, thereby meeting the integral structure requirement of the CPG column a and the CPG carrier plate 1.

Because the aperture is smaller, if the CPG column a adopts a process of preparing in advance in the CPG carrier section 112, the operation requirement is more strict, and the external factors can cause the difference in the installation accuracy of the CPG column a in each synthesis hole 11 during installation, so that the CPG air resistance is different, and finally the synthesis efficiency and quality of the DNA strand are affected.

In this embodiment, the carrier plate body 10 is made of a high-density PP material and is integrally manufactured by an injection molding process, and in this way, firstly, stability of the preparation process and economy of the preparation cost are ensured, and in order to ensure subsequent cleaning efficiency and reduce the dosage of the cleaning agent, a teflon coating is sprayed on the inner surface of the synthesis hole 11, so as to reduce the wall hanging phenomenon of the synthesis agent.

Referring to fig. 5, in this embodiment, the liquid separation plate 2 includes a liquid separation plate body 20, an upper end surface of the liquid separation plate body 20 is recessed inward to form a liquid separation cavity 23, liquid discharge channels 22 are arranged in the liquid separation cavity 23 in an array manner, in order to further improve the air resistance of the product, a filter membrane 4 is arranged in the liquid separation cavity 23, and a lower end of the synthetic hole 11 is abutted to the upper end surface of the filter membrane 4;

secondly, the lower end face of the liquid separation plate 20 is provided with a boss 21, in the practical application process, the lower end face of the CPG module is connected with a negative pressure system, the negative pressure system comprises a negative pressure device (generally an air extracting pump), an air extracting end pipe of the negative pressure device is connected with a waste liquid collecting box, the liquid separation plate 20 is inserted into the upper end face of the waste liquid collecting box, in order to enhance the air tightness during the insertion, the outer side of the boss 21 is sleeved with a sealing ring, meanwhile, in order to avoid the problem of sputtering when waste liquid flows through the waste liquid collecting box from the liquid separation plate 20, the liquid outlet end of the liquid discharging channel 22 extends into the waste liquid collecting box; the edge of the liquid separation plate body 20 is provided with a connecting hole 200 for connecting with the bolt 3, so that locking of the CPG carrier plate 1 and the liquid separation plate 2 is realized.

In the description of the present utility model, it should be understood that the terms "center," "lateral," "upper," "lower," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the utility model and simplify the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present utility model, unless otherwise indicated, the meaning of "a number" is two or more. In addition, the term "include" and any variations thereof are intended to cover a non-exclusive inclusion.

The utility model has been described in terms of embodiments, and the device can be modified and improved without departing from the principles of the utility model. It should be noted that all technical solutions obtained by equivalent substitution or equivalent transformation fall within the protection scope of the present utility model.

Claims

1. The CPG module with the 1 fmol-1 nmol synthesis yield comprises a CPG carrier plate (1), wherein the lower end surface of the CPG carrier plate (1) is connected with a liquid separation plate (2) through locking of bolts (7); the method is characterized in that: the CPG carrier plate (1) comprises synthesis holes (11) formed in the CPG carrier plate (1), CPG columns (a) are sintered in the synthesis holes (11), liquid discharge channels (22) are formed in the liquid separation plate (2), and the synthesis holes (11) correspond to the liquid discharge channels (22) one by one; the CPG column (a) is formed by premixing CPG powder and ultra-high molecular weight polyethylene powder, sieving the mixture into a synthetic hole and sintering the mixture, and the lower end of the CPG column (a) is flush with the lower end face of the CPG carrier plate (1).

2. The CPG module of claim 1, wherein the CPG module has a synthesis yield of 1fmol to 1 nmol: the synthesis hole (11) comprises a liquid inlet section (110), a transition section (111) and a CPG carrier section (112) which are sequentially arranged from top to bottom, the three sections are sequentially connected, and the CPG column (a) is sintered in the CPG carrier section (112).

3. The CPG module of claim 2, wherein the CPG module has a synthesis yield of 1fmol to 1nmol, and is characterized by: the CPG powder includes a mixture of non-silanized CPG powder and silanized CPG powder.

4. The CPG module of claim 2, wherein the CPG module has a synthesis yield of 1fmol to 1nmol, and is characterized by: the inner diameter of the liquid inlet section (110) is larger than that of the CPG carrier section (112), and the cross section of the transition section (111) in the vertical direction is in an inverted trapezoid shape.

5. The CPG module of claim 1, wherein the CPG module has a synthesis yield of 1fmol to 1 nmol: the CPG carrier plate (1) is prepared from a high-density PP material.

6. The CPG module of any of claims 1-5, wherein the CPG module has a 1fmol to 1nmol synthesis yield: the inner surface of the synthetic hole (11) is sprayed with a Teflon coating.

7. The CPG module of claim 6, wherein the CPG module has a synthesis yield of 1fmol to 1 nmol: the liquid separation plate (2) comprises a liquid separation plate body (20), a liquid separation cavity (23) is formed in the upper end face of the liquid separation plate body (20), liquid discharge channels (22) are arranged on the lower end wall of the liquid separation cavity (23) in an array mode, bosses (21) are arranged on the lower end face of the liquid separation plate body (20), and the lower ends of the liquid discharge channels (22) penetrate through the lower end faces of the bosses (21).

8. The CPG module of claim 6, wherein the CPG module has a synthesis yield of 1fmol to 1 nmol: a filter membrane (4) is arranged between the liquid separation plate (2) and the CPG carrier plate (1), and the filter membrane (4) is sintered on the lower surface of the CPG carrier plate (1) or the upper surface of the liquid separation plate (2) in an assembled state; or, the lower surface of the filter membrane (4) is pressed on the upper surface of the liquid separation plate (2), and the upper surface of the filter membrane (4) is pressed on the lower surface of the CPG carrier plate (1).

9. The CPG module of claim 1, wherein the CPG module has a synthesis yield of 1fmol to 1 nmol: CPG carrier plate (1) include carrier plate body (10), screw hole (12) that are used for holding bolt (7) are offered to the edge of carrier plate body (10) up end, and with kidney slot (13) of screw hole (12) crisscross setting.

10. The CPG module of claim 9, wherein the CPG module has a synthesis yield of 1fmol to 1 nmol: the middle position of the upper end face of the carrier plate body (10) is concavely provided with a concave cavity (14), and the synthesis holes (11) are arranged in the concave cavity (14) in an array mode.