CN218910384U

CN218910384U - Constant temperature tissue cell dissociation device

Info

Publication number: CN218910384U
Application number: CN202223229405.1U
Authority: CN
Inventors: 蒋明
Original assignee: Zhenjiang Zhongyi Biotechnology Co ltd
Current assignee: Zhenjiang Zhongyi Biotechnology Co ltd
Priority date: 2022-12-02
Filing date: 2022-12-02
Publication date: 2023-04-25
Anticipated expiration: 2032-12-02

Abstract

The utility model belongs to the technical field of cell dissociation, and particularly relates to a constant-temperature tissue cell dissociation device which comprises a device main body, wherein a reaction hole is formed in the upper surface of the device main body, a positioning hole is formed in the outer side of the reaction hole, a dissociation tube is arranged at the upper end of the reaction hole, a top cover is arranged at the upper end of the dissociation tube, a positioning pin is arranged at the upper end of the top cover, and a fixing hole is formed in the upper surface of the top cover. According to the utility model, by arranging the sealing plug, the dissociation tube, the connecting block, the bracket, the triangular block, the bulge, the fixing groove, the top cover, the locating pin, the temperature sensor and the heating component, the efficient dissociation of tissue cells can be realized, the dissociation efficiency and quality of the device to the cells can be improved through the contact friction effect of the triangular block and the bulge to the cells, the internal constant temperature effect can be kept in the cell dissociation process, the device has a simple structure, the dissociation operation is convenient to use, and the use effect of the dissociation device is improved.

Description

Constant temperature tissue cell dissociation device

Technical Field

The utility model relates to the technical field of cell dissociation, in particular to a constant-temperature tissue cell dissociation device.

Background

The tissue dissociation device is a device for dissociating biological tissues by using a tissue dissociation technology to obtain a high-activity and high-quality cell suspension. To obtain a cell suspension, the tissue dissociation device needs to destroy the extracellular matrix to release the cells from the extracellular matrix, and then needs to destroy the connection between the cells to obtain the cell suspension, and the dispersion method mainly comprises two methods, namely, directly crushing the tissue by a physical method and treating the tissue block by using reagents such as enzyme and the like, and dispersing the tissue block into single cells by a mechanical method after the treatment.

The prior art has the following problems:

in the use of the general dissociation device, the operation is troublesome, only the dissociation effect is carried out to the cells through simple rotation, the dissociation efficiency of the cells is slower, meanwhile, the inside cells can be insufficient in dissociation, the quality of the dissociated cells of the device is reduced, and the use effect is poor.

Disclosure of Invention

Aiming at the defects of the prior art, the utility model provides a constant-temperature tissue cell dissociation device, which solves the problems that the prior dissociation device is troublesome to operate and simple in dissociation mode, the dissociation efficiency of cells is slower, the dissociation of cells is insufficient, and the quality of dissociated cells of the device is reduced.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a homothermal tissue cell dissociation device, includes the device main part, the reaction hole has been seted up to the upper surface of device main part, the outside of reaction hole is provided with the locating hole, the upper end of reaction hole is provided with the dissociation pipe, the upper end of dissociation pipe is provided with the top cap, the upper end of top cap is provided with the locating pin, the fixed orifices has been seted up to the upper surface of top cap, the lower surface of top cap is connected with the connecting rod, the center department of connecting rod is connected with the sealing plug, the lower extreme of sealing plug is provided with the support that is connected with the connecting rod, the lower extreme of support is connected with the triangle, the inner wall of dissociation pipe is connected with the connecting block, the surface connection of triangle has the arch, the inside of reaction hole is provided with the fixed slot, the lower extreme of fixed slot is connected with the roating seat, the inside lateral wall of reaction hole is provided with heating element.

As a preferable technical scheme of the utility model, the inner diameter size of the positioning hole is matched with the inner diameter size of the fixing hole, and the fixing hole is fixedly connected with the positioning hole through a positioning pin.

As a preferable technical scheme of the utility model, the number of the brackets is a plurality of, and the brackets are uniformly distributed on the outer side of the connecting rod.

As a preferable technical scheme of the utility model, the number of the triangular blocks is a plurality, the triangular blocks are distributed at the lower end of the bracket at equal intervals, and the triangular blocks are fixedly connected with the bracket.

As a preferable technical scheme of the utility model, the number of the connecting blocks is a plurality, and the connecting blocks are uniformly distributed on the inner wall of the dissociation tube in an equiangular manner.

As a preferable technical scheme of the utility model, the upper end of the heating component is provided with a temperature sensor, and the temperature sensor is electrically connected with the heating component.

Compared with the prior art, the utility model provides a constant-temperature tissue cell dissociation device, which has the following beneficial effects:

1. this constant temperature tissue cell dissociation device is through setting up sealing plug, dissociation pipe, connecting block, support, triangular block and arch, can be in fact the high-efficient dissociation effect to tissue cell, in the use, the operator puts into the inside of dissociation pipe with the cell that needs to dissociate, covers the top cap, and through the drive effect of device main part, the dissociation pipe rotates at a high speed, the connecting block is carried inside cell along with the rotation of dissociation pipe, the cell rotates the in-process and can contact with inside triangular block and arch, through mutual frictional interaction, can improve the dissociation efficiency and the quality of device to the cell.

2. This constant temperature tissue cell dissociation device is through setting up fixed slot, top cap, fixed orifices, locating hole, locating pin, temperature sensor and heating element, not only can realize that the cell is dissociated the in-process and keep inside constant temperature effect, device simple structure after the improvement moreover is convenient for dissociate the use of operation, improves the result of use of dissociation device.

Drawings

FIG. 1 is a schematic diagram of a dissociation device according to the present utility model;

FIG. 2 is a schematic view of the internal structure of the dissociation tube;

FIG. 3 is a schematic diagram of a triangle block structure;

FIG. 4 is a schematic view of the internal structure of the reaction well.

In the figure: 1. a device body; 2. a reaction hole; 3. positioning holes; 4. a dissociation tube; 5. a top cover; 6. a positioning pin; 7. a fixing hole; 8. a connecting rod; 9. a sealing plug; 10. a bracket; 11. triangular blocks; 12. a connecting block; 13. a protrusion; 14. a fixing groove; 15. a rotating seat; 16. a temperature sensor; 17. and a heating assembly.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1 to 4, in the present embodiment: the utility model provides a homothermal tissue cell dissociation device, including device main part 1, reaction hole 2 has been seted up to the upper surface of device main part 1, the outside of reaction hole 2 is provided with locating hole 3, the upper end of reaction hole 2 is provided with dissociation pipe 4, the upper end of dissociation pipe 4 is provided with top cap 5, the upper end of top cap 5 is provided with locating pin 6, a fixed orifices 7 has been seted up to the upper surface of top cap 5 for the lower surface of top cap 5 is connected with connecting rod 8, the center department of connecting rod 8 is connected with sealing plug 9, a sealed use for dissociation pipe 4 is inside, and sealing plug 9 can rotate along with dissociation pipe 4, the lower extreme of sealing plug 9 is provided with the support 10 that is connected with connecting rod 8, the lower extreme of support 10 is connected with triangle piece 11, the inner wall connection of dissociation pipe 4 has connecting block 12, the surface connection of triangle piece 11 has protruding 13, the inside of reaction hole 2 is provided with fixed slot 14, the lower extreme connection of fixed slot 14 has rotary seat 15 for the high-speed rotation use of dissociation pipe 4, the inside lateral wall of reaction hole 2 is provided with heating element 17, realize the effect.

In this embodiment, the inner diameter of the positioning hole 3 is matched with the inner diameter of the fixing hole 7, and the fixing hole 7 is fixedly connected with the positioning hole 3 through the positioning pin 6, which mainly serves to ensure the stability of the top cover 5 in the use process of the device; the number of the brackets 10 is a plurality, the brackets 10 are uniformly distributed on the outer side of the connecting rod 8, and the structure is simple and reasonable and is easy to realize; the number of the triangular blocks 11 is a plurality, the triangular blocks 11 are equidistantly distributed at the lower end of the support 10, and the triangular blocks 11 are fixedly connected with the support 10 and mainly play a role in improving the dissociation quality and dissociation efficiency of cells in the dissociation tube 4; the bulges 13 are divided into two groups, the number of each group of bulges 13 is a plurality of, the bulges 13 are distributed on two sides of the triangular block 11 at equal intervals, and the uniformity of cell dissociation is realized through friction; the number of the connecting blocks 12 is a plurality, the connecting blocks 12 are uniformly distributed on the inner wall of the dissociation tube 4 at equal angles, so that the rotation speed difference between cells in the dissociation tube 4 is avoided, and the use effect of the dissociation device is improved; the upper end of the heating component 17 is provided with a temperature sensor 16, and the temperature sensor 16 is electrically connected with the heating component 17, so that the constant-temperature operation environment of the dissociation device is realized, and the use effect of the dissociation device is improved.

The working principle and the using flow of the utility model are as follows: when the dissociation device is used, an operator pours cell liquid into the inside of the dissociation tube 4, the dissociation tube 4 with the cell liquid is inserted into the inside of the reaction hole 2, the dissociation tube 4 is fixed by the fixing groove 14, the top cover 5 with the sealing plug 9 is covered at the upper end of the dissociation tube 4, the sealing plug 9 is plugged into the inside of the dissociation tube 4, the top cover 5 is sleeved with the dissociation tube 4, the fixing hole 7 on the surface of the top cover 5 is aligned with the positioning hole 3 by rotating the top cover 5, the top cover 5 is fixed with the positioning hole 3 by adopting the positioning pin 6, a power supply is connected, the starting device is started, the rotary seat 15 rotates with the dissociation tube 4, the sealing plug 9 rotates under the action of the dissociation tube 4, the connecting block 12 in the dissociation tube 4 rotates at a high speed, the cell rotates at a high speed to interact with the triangular block 11 and the bulge 13, the cell can dissociate, meanwhile, the temperature sensor 16 detects the temperature of the dissociation tube 4 in real time, and the temperature sensor 16 heats the dissociation tube at a lower temperature constant temperature assembly 17.

Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present utility model, and the present utility model is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present utility model has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims

1. A constant temperature tissue cell dissociation device, comprising a device body (1), characterized in that: the device is characterized in that the upper surface of the device main body (1) is provided with a reaction hole (2), the outer side of the reaction hole (2) is provided with a positioning hole (3), the upper end of the reaction hole (2) is provided with a dissociation pipe (4), the upper end of the dissociation pipe (4) is provided with a top cover (5), the upper end of the top cover (5) is provided with a positioning pin (6), the upper surface of the top cover (5) is provided with a fixing hole (7), the lower surface of the top cover (5) is connected with a connecting rod (8), the center of the connecting rod (8) is connected with a sealing plug (9), the lower end of the sealing plug (9) is provided with a support (10) connected with a connecting rod (8), the inner wall of the dissociation pipe (4) is connected with a connecting block (12), the surface of the triangle block (11) is connected with a bulge (13), the inner part of the reaction hole (2) is provided with a fixing groove (14), the lower end of the fixing groove (14) is connected with a rotating seat (15), and the inner side wall (17) of the reaction seat (2) is provided with a heating component (17).

2. A constant temperature tissue cell dissociation device as claimed in claim 1 wherein: the inner diameter of the positioning hole (3) is matched with the inner diameter of the fixing hole (7), and the fixing hole (7) is fixedly connected with the positioning hole (3) through the positioning pin (6).

3. A constant temperature tissue cell dissociation device as claimed in claim 1 wherein: the number of the brackets (10) is a plurality of, and the brackets (10) are uniformly distributed on the outer side of the connecting rod (8).

4. A constant temperature tissue cell dissociation device as claimed in claim 1 wherein: the number of the triangular blocks (11) is a plurality, the triangular blocks (11) are distributed at the lower end of the support (10) at equal intervals, and the triangular blocks (11) are fixedly connected with the support (10).

5. A constant temperature tissue cell dissociation device as claimed in claim 1 wherein: the protrusions (13) are divided into two groups, the number of the protrusions (13) in each group is a plurality, and the protrusions (13) are distributed on two sides of the triangular block (11) at equal intervals.

6. A constant temperature tissue cell dissociation device as claimed in claim 1 wherein: the number of the connecting blocks (12) is a plurality, and the connecting blocks (12) are uniformly distributed on the inner wall of the dissociation tube (4) in an equiangular manner.

7. A constant temperature tissue cell dissociation device as claimed in claim 1 wherein: the upper end of the heating component (17) is provided with a temperature sensor (16), and the temperature sensor (16) is electrically connected with the heating component (17).