CN218893659U

CN218893659U - Cassette disc

Info

Publication number: CN218893659U
Application number: CN202223451953.9U
Authority: CN
Inventors: 袁庆明; 陈居勇; 李芳敏; 祝君; 秦资
Original assignee: Shanghai 3D Medicines Co Ltd
Current assignee: Shanghai 3D Medicines Co Ltd
Priority date: 2022-12-22
Filing date: 2022-12-22
Publication date: 2023-04-21
Anticipated expiration: 2032-12-22

Abstract

The embodiment of the application provides a cartridge disk, which comprises: the probe reagent tube, the primer reagent tube, the disk body, probe reagent hole and primer reagent hole have been seted up to the disk body, probe reagent hole is suitable for detachably installs the probe reagent tube, primer reagent hole is suitable for detachably installs the primer reagent tube. Through setting up the probe reagent hole of demountable installation probe reagent pipe and the primer reagent hole of demountable installation primer reagent pipe, can freely select to dismantle different probe reagent pipes and primer reagent pipes of changing to with each general reagent cooperation of other gene capture objects, use same cartridge dish to realize the gene library preparation to corresponding gene capture object to different capture objects, thereby facilitated the preparation of gene library.

Description

Cassette disc

Technical Field

The embodiment of the application relates to the technical field of gene sequencing devices, in particular to a cartridge disc.

Background

Gene sequencing is an important technology for analyzing genomics data, has been increasingly expanded and deepened in clinical application, and a high-throughput sequencing technology (NGS) is used as a gene sequencing technology, and is widely applied to the fields of basic research, prenatal diagnosis, genetic disease diagnosis, tumor diagnosis, precise medical treatment and the like by virtue of the advantages of high throughput, high accuracy, abundant information and the like. However, not only is whole genome sequencing time and effort consuming, but it also generates much redundant and clinically worthless data, so capturing specific regions for detection is the mainstream treatment protocol in clinical use today.

The second generation hybrid capture technology mainly comprises multiple PCR capture technology, solid phase chip technology, liquid phase probe capture technology, molecular inversion capture technology and other technologies. The liquid phase hybridization capture technology is a technology which enables target DNA fragments to be effectively enriched through the reaction of biotin and streptavidin on magnetic beads by directly hybridizing the target DNA fragments with a probe with biotin marks in a solution, and the obtained target DNA fragments have good uniformity and repeatability, so that the target DNA fragments are widely applied.

The liquid phase hybridization capture technology requires library construction, and the library construction requires the use of a cartridge having a cartridge tray, and different capture objects require the use of different cartridge trays, resulting in various inconveniences.

Therefore, how to construct libraries more conveniently becomes a technical problem that needs to be solved by those skilled in the art.

Disclosure of Invention

In view of this, the present embodiments provide a cartridge to use the same cartridge for different capture objects.

In order to achieve the above purpose, the embodiment of the present application provides the following technical solutions:

the embodiment of the application provides a cartridge disk, which comprises:

a probe reagent tube;

primer reagent tube;

the probe reagent hole is suitable for detachably mounting the probe reagent tube, and the primer reagent hole is suitable for detachably mounting the primer reagent tube.

Optionally, the magnetic bead reagent tube is further included, the disk body includes:

the main disk body is provided with a magnetic bead disk hole;

the magnetic bead disk body is arranged in the magnetic bead disk hole, detachably mounted on the main disk body and provided with the magnetic bead reagent tube.

Optionally, the probe reagent hole and the primer reagent hole are both opened on the magnetic bead disk body.

Optionally, the magnetic bead disk is rotatably mounted to the main disk and adapted to rotate about a magnetic bead disk axis of the magnetic bead disk.

Optionally, the magnetic bead disk further comprises a quality control tube, and the quality control tube is further installed on the magnetic bead disk.

Optionally, the device further comprises a reaction reagent tube and a capturing washing reagent tube, the main tray body is further provided with a reaction tray hole, and the tray body further comprises:

the reaction disk body is arranged in the reaction disk hole, is installed in the main disk body, and is provided with the reaction reagent tube and the capturing washing reagent tube.

Optionally, the reaction disk is rotatably mounted to the main disk and adapted to rotate about a reaction disk axis of the reaction disk.

Optionally, the main disc body is further provided with a linear reagent disc hole, and the disc body further comprises:

the linear reagent disk body is arranged in the linear reagent disk hole, is installed in the main disk body, and is provided with reagent pipes which are arranged linearly.

Optionally, the magnetic bead disk is rotatably mounted on the main disk and is adapted to rotate around a magnetic bead disk axis of the magnetic bead disk, a first distance between a center of each of the magnetic bead reagent tubes and a center of the magnetic bead disk, and a second distance between a center of each of the reaction reagent tubes and a center of the capture wash reagent tube and a center of the reaction disk are the same.

Optionally, the reagent tube of the linear reagent disk comprises:

a first row of reagent tubes arranged along a first row of the linear reagent tray;

the second row of reagent tubes are arranged along a second row of the linear reagent tray body, the first row is parallel to the second row, and the distance between the liquid taking center of the first row of reagent tubes and the liquid taking center of the second row of reagent tubes is equal to twice the first distance or the second distance;

the connecting line of the center of the magnetic bead disk body and the center of the reaction disk body coincides with the central line between the first row of reagent pipes and the second row of reagent pipes.

Reagent tubes are mounted in a double line arrangement with a spacing between the double lines twice the first distance and the second distance.

Optionally, the magnetic bead reagent tube of the magnetic bead disk body 4 is a film sealing reagent tube.

Optionally, the magnetic bead disk body is provided with a magnetic bead disk guide post, and the magnetic bead disk guide post protrudes out of the disk surface of the magnetic bead disk body and is suitable for indicating the film sealing information of the film sealing reagent tube.

Optionally, the trapping washing reagent tube of the reaction disk body is a film sealing reagent tube.

Optionally, the reaction tray body is provided with a reaction tray guide post, and the reaction tray guide post protrudes out of the tray surface of the tray body and is suitable for indicating the film sealing information of the film sealing reagent tube.

The embodiment of the application provides a cartridge disk, which comprises: the probe reagent tube, the primer reagent tube, the disk body, probe reagent hole and primer reagent hole have been seted up to the disk body, probe reagent hole is suitable for detachably installs the probe reagent tube, primer reagent hole is suitable for detachably installs the primer reagent tube. Thus, according to the cartridge disc provided by the embodiment of the application, through arranging the probe reagent hole for detachably installing the probe reagent tube and the primer reagent hole for detachably installing the primer reagent tube, when the gene library is prepared, the probe reagent tube or the primer reagent tube can be freely selected and removed for replacement according to different gene capturing objects, so that the cartridge disc is matched with all universal reagent tubes of other gene capturing objects, and the gene library preparation of the corresponding gene capturing objects is realized by using the same cartridge disc according to different capturing objects, so that the preparation of the gene library is facilitated; meanwhile, the waste of reagents in the cartridge tray can be reduced.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present application, and that other drawings may be obtained according to the provided drawings without inventive effort to a person skilled in the art.

FIG. 1 is a schematic diagram of a cartridge according to an embodiment of the present disclosure;

FIG. 2 is a schematic diagram of a magnetic bead disk body of a cartridge according to an embodiment of the present disclosure;

FIG. 3 is a schematic view of another structure of a cartridge according to an embodiment of the present disclosure;

FIG. 4 is a schematic structural view of a reaction plate body of the cartridge plate according to the embodiment of the present application;

FIG. 5 is a top view of a cartridge tray provided in an embodiment of the present application;

fig. 6 is another schematic structural diagram of a magnetic bead disk body of the cartridge disk according to the embodiment of the present application.

Wherein, 1-the tray body; 2-a main disc body; 11-a magnetic bead disk hole; 21-probe reagent wells; 22-probe reagent tube; 31-primer reagent wells; 32-primer reagent tube; 4-a magnetic bead disk body; 41-magnetic bead reagent tube; 42-a quality control tube; 5-reaction tray body; 51-reagent tube; 52-capturing a wash reagent tube; 6-a linear reagent tray; 71-sealing the film; 72-a magnetic bead disk guide post; 73-reaction disk guide post.

Detailed Description

The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all, of the embodiments of the present application. All other embodiments, which can be made by one of ordinary skill in the art without undue burden from the present disclosure, are within the scope of the present disclosure.

As known from the background art, the existing cartridge has no flexibility, and the immobilized probes and primers are not convenient for constructing capture libraries of various target areas, so as to solve the foregoing technical problems, the embodiments of the present application provide a cartridge, refer specifically to fig. 1-3, and fig. 1 is a schematic structural diagram of the cartridge provided in the embodiments of the present application; FIG. 2 is a schematic diagram of a magnetic bead disk body of a cartridge according to an embodiment of the present disclosure; fig. 3 is another schematic structural diagram of a cartridge according to an embodiment of the present application.

As shown in the drawings, a cartridge provided in an embodiment of the present application may include:

a probe reagent tube 22;

primer reagent tube 32;

the probe reagent hole 21 and the primer reagent hole 31 are formed in the tray body 1, the probe reagent hole 21 is suitable for detachably mounting the probe reagent tube 22, and the primer reagent hole 31 is suitable for detachably mounting the primer reagent tube 32.

The disc body 1 refers to the rest part of each reagent tube in the cartridge disc provided in the embodiment of the present application, the disc body 1 is provided with a probe reagent hole 21 and a primer reagent hole 31, in some embodiments, the structures of the probe reagent hole 21 and the primer reagent hole 31 may be the same, so that the connection structure of the probe reagent tube 22 and the primer reagent tube 32 is the same, and thus, the different reagent holes and reagent tubes may be distinguished by colors and the like, so that the processing difficulty of the disc body 1, the probe reagent tube 22 and the primer reagent tube 32 may be reduced; in other embodiments, the connection structure between the probe reagent well 21 and the primer reagent well 31 is different, and correspondingly, the structures of the probe reagent tube 22 and the primer reagent tube 32 are also different, and the probe reagent tube 22 corresponds to the probe reagent well 21, and the primer reagent tube 32 corresponds to the primer reagent well 31, so that the reagent tubes can be conveniently and quickly matched with the corresponding reagent wells, and the error-filling reagent tube and the determination reagent tube can be prevented.

It is to be understood that any specific structure capable of implementing the above-described detachable mounting of the probe reagent tube 22 and the primer reagent tube 32 can implement the present embodiment, and solve the technical problem solved by the present embodiment, and therefore, the present embodiment is not limited to the specific structure implementing the above-described detachable mounting, and only for more clearly describing the present embodiment, in a specific implementation, may specifically be: the probe reagent hole 21 and/or the primer reagent hole 31 are provided with a female buckle, the probe reagent tube 22 and/or the primer reagent tube 32 are provided with male buckles, the probe reagent tube 22 and/or the primer reagent hole 32 can be fastened between the male buckles and the female buckle to be installed on the probe reagent hole 21 and/or the primer reagent hole 31, in another specific embodiment, the probe reagent hole 21 and/or the primer reagent hole 31 can be a threaded hole, and the probe reagent tube 22 and/or the primer reagent tube 32 are provided with threads and can be connected (installed) on the probe reagent hole 21 and/or the primer reagent hole 31 in a threaded manner.

It is to be understood that the above-described connection structure is a structure in which connection fixation is achieved when the probe reagent tube 22 is attached to the probe reagent well 21, and a structure in which connection fixation is achieved when the primer reagent tube 32 is attached to the primer reagent well 31.

Of course, the probe reagent tube 22 includes probe reagents therein, and different probe reagents for capturing different capture objects are contained in different probe reagent tubes 22, and different probe reagent tubes 22 have the same structure so as to be capable of being mounted in the same probe reagent well 21; likewise, the different primer reagent tubes 32 have the same structure so as to be capable of being mounted in the same primer reagent hole 31, so that the same cartridge tray can mount the probe reagent tube 22 having different probe reagents and the primer reagent tube 32 having different primer reagents, and the probe reagent tube 22 and the primer reagent tube 32 corresponding thereto are selectively mounted according to a specific capturing object, avoiding that the cartridge tray cannot be used because the gene capturing object to which the probe reagent tube 22 and the primer reagent tube 32 are directed is different from the target capturing object, requiring additional replacement of the cartridge tray corresponding to the target capturing object, and wasting the entire cartridge tray and all reagents thereon.

A specific application scenario is provided below to further explain the reagent waste:

it is easy to understand that in actual production, many target capturing objects exist in the actual application process, so that corresponding needs are prepared for various capturing objects, after capturing the target objects, genetic library construction may not be performed in a short time, the preparation for other capturing objects prepared before is temporarily not used, the storage of the whole cartridge tray occupies more space, and because a certain temperature condition is required for reagent storage, the storage requirement is higher, and more energy is occupied; in addition, when the storage requirement is not satisfied, reagent damage may be caused, reagent waste is caused, and all reagents in the cartridge tray may be wasted.

By detachably attaching the probe reagent tube 22 and the primer reagent tube 32 of the cartridge to the disk body 1, when preparing the article, if only one cartridge is required, only one cartridge may be prepared, and a plurality of probe reagent tubes 22 and a plurality of primer reagent tubes 32 which may be used may be prepared, and after determining the target gene capturing object, the corresponding reagent tube is selected from the plurality of probe reagent tubes 22 and the plurality of primer reagent tubes 32 which are prepared in advance, and attached to the corresponding position of the disk body, and used in cooperation with the universal reagent tube in the cartridge, thereby realizing gene library preparation.

In the above manner, if gene banking is not performed in a short time later, there is a need for storage of the unused probe reagent tube 22 and primer reagent tube 32 and a risk of damage waste, however, waste of the entire cartridge tray (including the tray body 1, the universal reagent tube, and the unused probe reagent tube 22 and primer reagent tube 32) can be avoided; on the other hand, the volume of the probe reagent tube 22 and the primer reagent tube 32 is smaller than that of a complete cartridge disk, so that the cartridge disk is convenient to store, and the required storage space and the required energy source can be reduced, thereby reducing the damage probability of the reagent.

Thus, according to the cartridge tray provided by the embodiment of the application, by arranging the probe reagent hole 21 for detachably mounting the probe reagent tube 22 and the primer reagent hole 31 for detachably mounting the primer reagent tube 32, when preparing a gene library, the probe reagent tube 22 or the primer reagent tube 32 can be freely selected and replaced according to different gene capturing objects, so that the cartridge tray is matched with all universal reagent tubes of other gene capturing objects, and the gene library preparation of the corresponding gene capturing objects is realized by using the same cartridge tray according to different capturing objects, so that the preparation of the gene library is facilitated; meanwhile, the waste of reagents in the cartridge tray can be reduced.

In order to better store various reagents in the cartridge, in a specific embodiment, the tray body 1 of the cartridge provided in the embodiment of the present application may include:

the main disk body 2 is provided with a magnetic bead disk hole 11;

the magnetic bead disk body 4 is provided in the magnetic bead disk hole 11, detachably mounted on the main disk body, and mounted with the magnetic bead reagent tube 41.

It will be readily appreciated that the optimal storage temperatures for the various reagents in the cartridge are not the same, such as: the optimal storage temperature of the magnetic bead reagent in the magnetic bead reagent tube 41 is 4 ℃, and the optimal storage temperature of other reagents is-20 ℃, so that different reagents need to be stored in a partitioning mode for better storing the reagents, and according to the cartridge tray provided by the embodiment of the utility model, the magnetic bead tray holes 11 are formed in the tray body, the magnetic bead tray body 4 can be detachably arranged, and the magnetic bead reagent tube 41 is arranged in the magnetic bead tray body 4, so that when the reagents are stored, the magnetic bead tray body 4 can be detached from the main tray body 2 and can be independently placed into a temperature zone for storage, and the remaining reagents with the cartridge tray can be stored in the temperature zone, so that different reagents are stored in different temperature zones according to the optimal storage temperature of the reagents, and each reagent in each cartridge tray can be better stored; meanwhile, since each of the magnetic bead reagent tubes 41 needs to be stored in a warm area having a temperature of 4 ℃, by installing each of the magnetic bead reagent tubes 41 in the magnetic bead disk body 4 and detachably installing the magnetic bead disk body 4 in the main disk body 2, the common storage and use installation of all the magnetic bead reagent tubes 41 can be rapidly realized, the operation is simplified, and the individual magnetic bead reagent tubes 41 are prevented from being lost in the storage process.

It is to be readily understood that any specific structure capable of realizing the above-described detachably mounted magnetic bead disk body 4 can realize the present embodiment, solving the technical problems solved by the present embodiment, and thus, the present embodiment is not limited to realizing the above-described detachably mounted specific structure, which can be referred to the above description about the probe reagent well 21, the primer reagent well 31, the probe reagent tube 22 and the primer reagent tube 32.

It should be noted that, the "the magnetic bead disk 4 is mounted with the magnetic bead reagent tube 41" includes both the magnetic bead disk 4 detachably mounting the magnetic bead reagent tube 41 and the magnetic bead disk 4 fixedly mounting the magnetic bead reagent tube 41, and when the magnetic bead reagent tube 41 is fixedly mounted, the magnetic bead disk 4 may be integrally formed with the magnetic bead reagent tube 41, for example: the magnetic bead reagent tube 41 may be a groove formed in the magnetic bead disk body 4, or the magnetic bead reagent tube 41 may be fixed to the magnetic bead disk body 4 by a fixed connection method such as adhesion.

Of course, in other embodiments, the magnetic bead disk 41 may be directly provided without the magnetic bead reagent tube 41 detachably attached to the disk 1, and the partition storage may be similarly realized by detaching and storing the magnetic bead reagent tubes 41.

In order to more reasonably utilize the space of the disk body, in a specific embodiment, the probe reagent holes 21 and the primer reagent holes 22 of the cartridge disk provided in the embodiment of the present application may be both opened in the magnetic bead disk body 4.

Thus, the magnetic bead reagent tube 41, the probe reagent tube 22 and the primer reagent tube 32 can be arranged on the magnetic bead tray body 4, and other types of reagent tubes can be arranged on other positions of the tray body, so that the reagent tubes can be placed in a partitioned manner, the placement of each reagent tube is more reasonable, the space of the tray body can be more reasonably utilized, and the corresponding reagent can be conveniently and rapidly acquired based on different regions when the preparation of the gene library is specifically carried out.

Of course, since the optimal storage temperatures of the probe reagent tube 22 and the primer reagent tube 32 are different from the optimal storage temperature of the magnetic bead reagent tube 41, the probe reagent tube 22 and the primer reagent tube 32 can be detached from the magnetic bead disk body 4 at the time of storage, the magnetic bead disk body 4 with the magnetic bead reagent tube 41 can be stored in a region having a temperature of 4 ℃, and the probe reagent tube 22 and the primer reagent tube 32 can be stored in a region having a temperature of-20 ℃, so that the magnetic bead reagent tube 41, the probe reagent tube 22 and the primer reagent tube 32 can be stored similarly well.

In order to improve convenience of gene banking and thus efficiency of gene banking, in a specific embodiment, the magnetic bead disk body 4 of the cartridge disk provided in the embodiment of the present application may be rotatably mounted on the main disk body 2 and adapted to rotate around the magnetic bead disk axis of the magnetic bead disk body 4.

Of course, the magnetic bead disk body 4 may be a circular turntable and have a center, so that the axis of the magnetic bead disk body is a straight line passing through the center and perpendicular to the disk surface of the magnetic bead disk body 4.

In other embodiments, the magnetic bead disk 4 may have other shapes, such as a regular polygon, so that a straight line passing through the center of the regular polygon and perpendicular to the disk surface of the magnetic bead disk 4 is the magnetic bead disk axis.

Therefore, when the gene library is built, the magnetic bead disk body 4 can be rotated, and the reagent which needs to be moved and used can be rotated to the target position, so that the corresponding reagent can be conveniently extracted by the pipette gun in the equipment provided with the cartridge disk, the moving mode of the pipette gun can be simplified, the convenience of the gene library is improved, the rotation of the magnetic bead disk body 4 and the movement of the pipette gun can be simultaneously carried out, and the efficiency of the gene library can be improved.

It is easy to understand that in the process of carrying out gene banking, the quality of intermediate products needs to be mastered at any time, in order to realize quality monitoring of the gene banking process and make full use of the space of the tray body, in a specific embodiment, the cartridge tray provided in the embodiment of the present application may further include a quality control tube 42, and the magnetic bead tray body 4 may further be provided with the quality control tube 42.

It is easy to understand that the quality control tube 42, which is commonly known in the art as a QC tube, does not store reagents in advance before the library is built, but acquires intermediate products generated during the library building process, so that by performing quality measurement and control on the intermediate products (through a quality control module under the quality control tube 42), it is found whether the current library building step and the previous steps of the current library building step have errors, and whether the requirements of uniformity and the like are met.

In this way, by providing the mass control tube 42 to the bead disk 4, the mass control tube 42 can be stored together in the temperature partition of the bead disk 4, and since the mass control tube 42 is an empty tube before the warehouse is built, it is not necessary to limit specific temperature conditions, and therefore, by storing it in a higher temperature region, the energy consumed in storing the cartridge disk can be reduced; meanwhile, for the gene library building equipment, a sufficient space is arranged below the magnetic bead disc body 4 to be provided with a quality control module (QC module), so that the quality control module is convenient to be matched with the quality control tube 42 for quality monitoring; on the other hand, the reagent tubes can be placed in a partitioned mode, so that the reagent tubes are placed more reasonably, the space of the tray body can be utilized more reasonably, and corresponding reagents can be acquired based on different areas rapidly when the gene library preparation is carried out.

In order to further improve the efficiency of gene library construction and improve the space utilization rationality of the cartridge, the embodiment of the present application further provides a cartridge, please refer to fig. 4, fig. 4 is a schematic structural diagram of the reaction disk body 5 of the cartridge provided in the embodiment of the present application.

As shown in the figure, in a specific embodiment, the cartridge tray provided in the present application further includes a reagent tube 51 and a capturing washing reagent tube 52, the main tray body 2 further includes a reaction tray hole, and the tray body 1 further includes: the reaction disk body 5 is provided in the reaction disk hole, is attached to the main disk body 2, and is provided with the reaction reagent tube 51 and the trapping washing reagent tube 52.

It should be noted that the reagent tube 51 is not pre-filled with reagent, and thus can be stored in any one of the temperature zones during the storage stage, and thus, in some embodiments, the reagent tube 51 may be disposed in the magnetic bead disk 4.

However, since the optimum storage temperature of the capture washing reagent is different from that of the magnetic bead reagent tube 41, there is a certain inconvenience in that the capture washing reagent tube 52 containing the capture washing reagent is provided on the magnetic bead disk body 4, and in the construction process, the pipette can repeat the steps of taking out the reagent from the reagent tube of the cartridge disk and adding the reagent to the reaction reagent tube 51, and in order to prevent contamination of the reagent in each reagent tube of the cartridge disk, it is generally necessary to wash the pipette with the capture washing reagent, so that the reaction reagent tube 51 is provided in the vicinity of the capture washing reagent tube 52, and the reagent can be washed more rapidly after the pipette has put the reagent into the reaction reagent tube 51, thereby significantly increasing the construction speed, and in this way, the reaction reagent tube 51 is provided on the reaction disk body 5, and the capture washing reagent tube 52 can be more closely provided than the reaction reagent tube 4, thereby increasing the gene construction speed.

It is noted that the reaction disk 5 also belongs to the disk of a cartridge, which in some embodiments may be simply a logically divided area of the disk, including the reagent tubes 51 and the capture wash reagent tubes 52.

Of course, in other embodiments, the reaction tray 5 may have a separate physical structure, and the reaction tray 5 may be detachably mounted to the main tray 2, so that the reaction tray 5 may be separately stored in a warm area adapted to store the captured wash reagent tubes 52, reducing the volume put into the warm area.

In order to improve convenience of gene banking and thus efficiency of gene banking, in a specific embodiment, the reaction disk body 5 of the cartridge disk provided in the embodiment of the present application may be rotatably mounted to the main disk body 2, and adapted to rotate around a reaction disk shaft of the reaction disk body 5.

Of course, the reaction disk 5 may be a circular turntable, and has a center, so that the reaction disk axis is a straight line passing through the center and perpendicular to the disk surface of the reaction disk 5.

In other embodiments, the reaction disk 5 may have other shapes, such as a regular polygon, and thus, a straight line passing through the center of the regular polygon and perpendicular to the disk surface of the reaction disk 5 is the reaction disk axis.

In this way, when the gene library is built, the reaction disc body 5 can be rotated to realize that the reagent which needs to be moved and used is rotated to the target position, so that the corresponding reagent can be conveniently extracted by the pipette in the equipment installed by the cartridge disc, the moving mode of the pipette can be simplified, and the convenience of the gene library is improved; and the rotation of the reaction disk body 5 and the movement of the pipetting gun can be performed simultaneously, and the efficiency of gene library establishment can be improved.

In order to reduce the pipetting difficulty, in a specific embodiment, the cartridge tray provided in the embodiment of the present application further has a linear reagent tray hole, and the tray body 1 further includes:

a linear reagent disk 6 provided in the linear reagent disk hole and mounted on the main disk body 2, and having reagent tubes arranged in a linear manner.

It is easy to understand that the kit provided in this application necessarily includes all the reagent tubes required for completing the library construction, and since the temperature area suitable for storing the magnetic bead reagent tubes 41 in the magnetic bead tray body 4 is different from the temperature area suitable for storing most reagent tubes, most reagent tubes can only be arranged in the reaction tray body 5, which can cause the overlarge area of the reaction tray body 5, the reagent tubes are too crowded, the precision requirements for moving the pipette and rotating the reaction tray body 5 are too high, and extraction errors easily occur, therefore, the kit tray provided in this application further includes the linear reagent tray 6, so that the reagent tubes of the reaction tray body 5 can be shared by the linear reagent tray 6, and the reaction tray body 5 only needs to have the reagent tubes 51 and a small amount of capture and washing reagent tubes 52.

Meanwhile, in the process of library establishment, the pipetting of the reagent tubes in the linear reagent disk 6 only relates to the linear movement of the pipetting gun, but not to the nonlinear movement of the pipetting gun and the rotation of the turntable, so that pipetting is easy, and the greatest reagent tubes are arranged linearly, so that the overall difficulty of pipetting steps in library establishment can be reduced, and meanwhile, the linear reagent disk 6 with the reagent tubes arranged linearly is simple in structure and can be convenient for mechanical production.

Of course, in some embodiments, the linear reagent disk 6 may be only a logically divided area on the main disk body 2, so that the production difficulty may be reduced; in other embodiments, the linear reagent disk 6 may be physically detachably mounted to the main disk body 2, so that the linear reagent disk 6 may be separately stored in a temperature zone suitable for storage, and the volume placed in the temperature zone may be reduced, which may reduce the loss of energy and the required space.

In order to further improve the warehouse-building efficiency, in an embodiment, please refer to fig. 5, fig. 5 is a top view of a cartridge tray provided in an embodiment of the present application.

As shown in the drawing, in the cartridge tray provided in the embodiment of the present application, the magnetic bead tray body 4 is rotatably mounted on the main tray body 2, and is adapted to rotate around the axis of the magnetic bead tray body 4, a first distance between the center of each magnetic bead reagent tube 41 and the center of the magnetic bead tray body 4, and a second distance between the center of each reaction reagent tube 51 and the center of the capture washing reagent tube 52 and the center of the reaction tray body 5 are the same.

Of course, the reaction disk 5 also rotates about the reaction disk axis, as shown, the first distance being shown as distance a, and the second distance being shown as distance b, and distance a being equal to distance b.

Therefore, by rotating the magnetic bead disc body 4 and the reaction disc body 5, the magnetic bead disc body 4 and the reaction disc body 5 are respectively provided with a reagent tube on the line A or the line B, and therefore, a pipette matched with the cartridge disc only needs to linearly move along the line A or the line B to align the reagent tube of the reagent to be extracted, and the reagent tube is not required to be aligned through secondary adjustment after moving to the magnetic bead disc body 4 or the reaction disc body 5, so that the pipetting speed is accelerated, and the warehouse building efficiency is improved.

In order to further improve the efficiency of library creation, in a specific embodiment, the embodiment of the present application provides a cartridge tray, the reagent tube of the linear reagent tray body 6 includes:

the line connecting the center of the magnetic bead disk 4 and the center of the reaction disk 5 coincides with the center line between the first row of reagent tubes and the second row of reagent tubes.

Like this, first row reagent pipe is located line A, the second row reagent pipe is located line B, and the rifle that moves through online A and line B can draw the reagent of all reagent pipes of linear reagent dish 6, through rotatory magnetic bead disk body 4 and reaction disk body 5 in advance, makes magnetic bead disk body 4 and reaction disk body 5 need be moved to A line and B line by the test tube of pipetting to make the rifle that moves through the linearity can accomplish the storehouse flow of building up.

In order to prevent the magnetic bead reagent from being contaminated, in a specific embodiment, the magnetic bead reagent tube 41 of the magnetic bead disk body 4 is a film sealing reagent tube.

As shown in fig. 4, in the magnetic bead disk body 4, for transporting and storing the magnetic bead reagent for a long time, the magnetic bead reagent tube 41 needs to be sealed, and a sealing film 71 is arranged at the tube opening of the magnetic bead reagent tube 41, wherein the sealing film can isolate air and liquid and can be punctured by the gun head of the pipetting gun, so that the magnetic bead reagent in the magnetic bead reagent tube 41 is protected while pipetting is not hindered.

Of course, the probe reagent tube 22 and the primer reagent tube 32 may be sealed for transportation and long-term storage of the probe reagent and the primer reagent, and it is easy to understand that the reaction reagent tube 51 and the quality control tube 42 are not preloaded with reagents, and thus do not need to be sealed, and of course, the probe reagent tube 22 and the primer reagent tube 32 need to be sealed separately for convenience of disassembly, and cannot be sealed together with the bead reagent tube 41.

In this way, the magnetic bead reagent tube 41 and the primer reagent tube 32 can be transported and stored for a long time.

In order to improve the accuracy of the sealing film, in a specific embodiment, the magnetic bead disk has a magnetic bead disk guiding post 72, and the magnetic bead disk guiding post 72 protrudes out of the disk surface of the magnetic bead disk body 4 and is suitable for indicating the sealing film information of the sealing film reagent tube.

The sealing information, that is, the position information of the sealing reagent tube, in some embodiments, the magnetic bead disk guide posts 72 may be rectangular columns, so as to identify the reagent tube to be sealed, in other embodiments, the magnetic bead disk guide posts 72 may be cylindrical, and by providing two or more magnetic bead disk guide posts 72, the same identification function is achieved.

Thus, when the magnetic bead disk body 4 is a circular disk or the like, and the shape of each reagent tube is not easily distinguished, and the reagent tubes which need to be sealed are included, the reagent tubes which need to be sealed are identified by the sealing information, and the sealing error of the reagent tubes can be avoided.

For transporting and storing the captured washing reagent for a long time, please refer to fig. 6 in an embodiment, fig. 6 is a schematic diagram of another structure of the magnetic bead disk body of the cartridge disk according to the embodiment of the present application.

As shown in the drawing, in the reaction disk 5, for transportation and long-term storage of the capture washing reagent, the capture washing reagent tube 52 needs to be sealed, and specific reference is made to the above description of the magnetic bead reagent tube sealing.

In order to improve the accuracy of the sealing film, in a specific embodiment, the reaction tray body is provided with a reaction tray guiding column 73, and the reaction tray guiding column 73 protrudes out of the tray surface of the tray body 1 and is suitable for indicating the sealing film information of the sealing film reagent tube.

It will be readily appreciated that the reaction disk guide posts 73 and the bead disk guide posts 72 function identically and are all reagent tubes for which a sealing film is required, wherein the reaction disk guide posts 73 are located on the reaction disk body 5, as described above with reference to the bead disk guide posts.

Although the embodiments of the present application are disclosed above, the present application is not limited thereto. Various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the utility model, and the scope of the utility model shall be defined by the appended claims.

Claims

1. A cartridge comprising:

a probe reagent tube;

primer reagent tube;

2. The cartridge tray of claim 1, further comprising a magnetic bead reagent tube, the tray body comprising:

the main disk body is provided with a magnetic bead disk hole;

3. The cartridge tray of claim 2, wherein the probe reagent wells and the primer reagent wells are both open to the magnetic bead tray body.

4. The cartridge of claim 2, wherein the magnetic bead disk is rotatably mounted to the main disk and adapted to rotate about a magnetic bead disk axis of the magnetic bead disk.

5. The cartridge of claim 2, further comprising a mass control tube, the magnetic bead disk body further mounted with the mass control tube.

6. The cartridge tray of claim 2, further comprising a reagent tube and a capture wash reagent tube, wherein the main tray body is further provided with a reaction tray hole, and wherein the tray body further comprises:

7. The cartridge tray of claim 6, wherein the reaction tray is rotatably mounted to the main tray and adapted to rotate about a reaction tray axis of the reaction tray.

8. The cartridge of claim 7, wherein the main tray body is further provided with a linear reagent tray hole, the tray body further comprising:

the linear reagent disk body is arranged in the linear reagent disk hole, is installed in the main disk body, and is provided with reagent pipes which are in linear arrangement.

9. The cartridge tray of claim 8, wherein the magnetic bead tray is rotatably mounted to the main tray and adapted to rotate about a magnetic bead tray axis of the magnetic bead tray, a first distance from a center of each of the magnetic bead reagent tubes to a center of the magnetic bead tray, and a second distance from a center of each of the reagent tubes and a center of the capture wash reagent tube to a center of the reaction tray.

10. The cartridge tray of claim 9, wherein the reagent tube of the linear reagent tray body comprises:

11. The cartridge tray of claim 2, wherein the magnetic bead reagent tube of the magnetic bead tray body is a film-sealing reagent tube.

12. The cartridge tray according to claim 11, wherein the magnetic bead tray body is provided with a magnetic bead tray guide post protruding from a tray surface of the magnetic bead tray body, adapted to indicate film sealing information of the film sealing agent tube.

13. The cartridge tray according to claim 6, wherein the trapping wash reagent tube of the reaction tray body is a film-sealing reagent tube.

14. The cartridge tray of claim 13, wherein the reaction tray body has a reaction tray guide post protruding from a tray surface of the tray body adapted to indicate the envelope information of the envelope reagent tube.