CN218879896U - Bacterial biofilm on culture solution plate trades gets device - Google Patents
Bacterial biofilm on culture solution plate trades gets device Download PDFInfo
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- CN218879896U CN218879896U CN202223200223.1U CN202223200223U CN218879896U CN 218879896 U CN218879896 U CN 218879896U CN 202223200223 U CN202223200223 U CN 202223200223U CN 218879896 U CN218879896 U CN 218879896U
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Abstract
The utility model relates to a medical treatment experimental facilities technical field discloses a bacterial biofilm on culture solution board trades gets device, through attached bacterial biofilm with the bacterial biofilm cultivates the inner wall of cup, effectually played the guard action to the bacterial biofilm, when wasing, can directly cultivate the framework board with the bacterial biofilm of placing in the culture solution inboard and mention, the culture solution inboard of renewal, shorten operating time greatly, reduce the operation degree of difficulty, and improve the survival rate of biofilm, be applicable to the high flux and survey the biofilm.
Description
Technical Field
The utility model relates to a medical treatment experimental facilities technical field specifically is a bacterial biofilm on culture solution board trades gets device.
Background
Many acute bacterial infectious diseases are often caused by non-adhering (planktonic) pathogenic bacteria, such as chronic otitis media, chronic tonsillitis, endocarditis, and urinary tract infections, which are generally treated effectively with antibiotics.
The biofilm refers to a multicellular colony aggregated membranous substance formed by the growth of microorganisms in certain materials or tissues through intercellular adhesion and the wrapping of the microorganisms by extracellular polysaccharide matrixes generated and secreted by the microorganisms, is closely related to the self-protection function of bacteria, and the formed biofilm is also considered to be one of virulence factors of pathogenic bacteria, so that the biofilm generated at an infection part is also one of very important reasons for delaying the disease condition of a patient in the treatment of clinical infection. In addition, many biofilm-producing pathogens can also form biofilms on surfaces of various medical devices and instruments, such as surgical instruments and biomimetic implants, which can cause nosocomial infections in patients if not effectively prevented and removed. It is considered that 65% to 80% of infections are associated with biofilms, such as endocarditis, osteomyelitis, urinary tract infections and chronic lung infections, which impose a great burden on public health safety issues. The reason for the massive infection is that cells in the biofilm state are often phenotypically and physiologically different from planktonic cells, and cells in mature biofilms have greater tolerance and resistance to antibiotics, often 100-1000 times the MIC of the planktonic state. Thus, biofilms are an indispensable part of research on colonization and pathogenicity of bacteria.
In the process of culturing bacterial biofilms, a 96-well plate made of plastic is usually used for quantitative determination of crystal violet, and in the process of culturing, part of biofilms are easily lost due to membrane washing and liquid replacement, so that the result of quantitative determination is inaccurate, and the result judgment of further experiments is influenced, therefore, an effective method is needed for reducing bacterial biofilms slipping and leaving holes. The necessary operation of the conventional culture and determination process at present often causes defect and loss of partial biomembranes, and how to accurately determine the content of the biomembranes is a problem for researchers researching pathogenic microorganisms. The main method adopted in the current experiment is to stain the biofilm on a 96-well plate by crystal violet, and measure the OD value to obtain the biofilm formation amount. However, this method mainly faces the following two problems: (1) After the old culture medium is removed in the culture process, the culture hole needs to be cleaned by PBS for 2-3 times, and the biological membrane can be partially or completely removed from the hole due to the impact force of the liquid when the fresh culture medium is added, the crystal violet is dyed and the crystal violet dye solution is discarded after the dyeing in the process. (2) Cleaning and liquid changing are long in time consumption because liquid needs to be slowly injected into the holes in order to reduce the liquid impact force; (3) In the existing method, transcriptome and proteomics researches are carried out by adhering a circular polycarbonate membrane on a solid flat plate for culture, taking down the membrane after the culture is finished, putting the membrane into an EP tube, stripping a biological membrane, and taking out the polycarbonate membrane, so that the whole process is long in time consumption and easy to pollute. (4) The method is time-consuming and labor-consuming, even the skilled experimenter often needs 1 hour of operation to complete the liquid changing and determination operation on the premise of keeping the integrity of the biological membrane as much as possible, and the experiment intensity is high.
SUMMERY OF THE UTILITY MODEL
In order to overcome the defect that above-mentioned prior art exists, the utility model aims to provide a device is traded to bacterium biomembrane on culture solution board to make the biomembrane flow through the washing liquid easily, the biomembrane is damaged easily and trade long technical problem of time among the solution prior art in trading the bacterium biomembrane.
The utility model discloses a realize through following technical scheme:
a bacterial biofilm replacing device on a culture solution plate comprises a bacterial biofilm culture framework plate; the bacterial biofilm culture framework plate comprises a plurality of groups of bacterial biofilm culture strip-shaped plates which are spliced in parallel; each group of bacterial biofilm culturing strip-shaped plates comprise a plurality of bacterial biofilm culturing blocks, a bacterial biofilm culturing cup is arranged in each bacterial biofilm culturing block, the bacterial biofilms are attached to the inner wall of the bacterial biofilm culturing cup, the bacterial biofilm culturing cup is placed in a solution cup of a culture solution plate, and solution in the solution cup is in contact with the bacterial biofilms through the bacterial biofilm culturing cup.
Preferably, be equipped with two sets of fixed buckles on the bacterial biofilm culture framework board, one of them group of fixed buckle is fixed on the first bacterial biofilm culture bar of bacterial biofilm culture framework board, and another group of fixed buckle is fixed on the last bacterial biofilm culture bar of bacterial biofilm culture framework board, fixed buckle is used for fixed connection in the both sides draw-in groove of culture solution board.
Preferably, fixed buckle is V type structure, and fixed buckle one end is 75 contained angles with the bacterial biofilm culture plate and is connected, and the other end is the level setting.
Preferably, both sides of the bacterial biofilm culture strip-shaped plate are respectively provided with a replacing support handle.
Preferably, folding indentations are arranged between each group of bacterial biofilm culture strip plates and between each group of bacterial biofilm culture blocks, and the folding indentations are distributed in a dotted line and used for independently disassembling the bacterial biofilm culture blocks.
Preferably, one side of each bacterial biofilm culture block is provided with a lifting belt for lifting the single bacterial biofilm culture block.
Preferably, the bacterial biofilm culture cup is of a semi-permeable membrane truncated cone structure, and a polycarbonate membrane is adopted as a material.
Preferably, the bacterial biofilm culturing cup is located at the central position of the bacterial biofilm culturing block.
Preferably, the number of bacterial biofilm culture cups in the bacterial biofilm culture framework plate corresponds to the number of solution cups in the culture solution plate.
Preferably, the cup diameter of the solution cup of the culture plate is smaller than the plate diameter of the bacterial biofilm culture block.
Compared with the prior art, the utility model discloses following profitable technological effect has:
the utility model provides a device is got instead to bacterial biofilm on culture solution board, through attaching the inner wall that the cup was cultivateed to bacterial biofilm, bacterial biofilm cultivates the cup and has played the guard action to bacterial biofilm, when, bacterial biofilm cultivates in the solution cup that the cup ingresses the culture solution board, the solution cup cultivates cup and bacterial biofilm contact through bacterial biofilm, when wasing or getting the culture solution board instead, bacterial biofilm passes through bacterial biofilm and cultivates the solution separation in cup and the culture solution board solution cup, the damage that the solution of biofilm in culture solution board solution cup caused to the biofilm through the washing liquid outflow and in wasing has been avoided, the percentage of reserving to improving the biofilm is improved.
Furthermore, two groups of fixing buckles are arranged on the bacterial biofilm culturing framework plate, so that the bacterial biofilm culturing framework plate is effectively fixed in clamping grooves on two sides of the culture solution plate, and meanwhile, the fixation is conveniently released.
Further, fixed buckle is V type structure, and fixed buckle one end is 75 contained angles with the bacterial biofilm culture board and is connected, and the other end is the level setting, is convenient for cultivate the bacterial biofilm and constructs the board and fix in the both sides draw-in groove of culture solution board, also is convenient for remove fixedly simultaneously.
Furthermore, the two sides of the bacterial biofilm culturing strip-shaped plate are respectively provided with a replacing support handle, so that the bacterial biofilm culturing strip-shaped plate can be conveniently lifted, and the replacing time of the bacterial biofilm is reduced.
Furthermore, be equipped with between every group's bacterial biofilm culture bar shaped plate and between every bacterial biofilm culture piece and roll over the indentation, it is the dotted line distribution to roll over the indentation for dismantle bacterial biofilm culture piece alone, can carry out nimble dismantlement to bacterial biofilm culture plate or bacterial biofilm culture piece as required.
Furthermore, one side of each bacterial biofilm culture block is provided with a lifting belt for lifting the single bacterial biofilm culture block, and the bacterial biofilms are replaced.
Further, the bacterial biofilm culture cup is of a semi-permeable membrane truncated cone structure, and a polycarbonate membrane is adopted as a material, so that a solution can permeate into the bacterial biofilm culture cup, but the bacterial biofilm culture cup has a blocking effect, and the bacterial biofilm culture cup is convenient to extract.
Drawings
FIG. 1 is a schematic structural view of a bacterial biofilm changer according to the present invention;
FIG. 2 is a schematic side view of the bacterial biofilm culturing plate and the replacement holder of the present invention;
FIG. 3 is a schematic top view of the bacterial biofilm culturing plate of the present invention;
FIG. 4 is a schematic side view of the bacterial biofilm culture plate of the present invention;
FIG. 5 is a schematic structural view of the device for replacing bacterial biofilm of the present invention;
FIG. 6 is a schematic structural view of the EP tube placed on the bacterial biofilm culturing plate of the present invention.
In the figure: 1-bacterial biofilm culture framework plate; 2, fixing a buckle; 3, replacing the support handle; 4-bacterial biofilm culture block; 5-folding and impressing; 6-bacterial biofilm culturing cup; 7-lifting the pull belt; 8-plates of culture medium; 10-EP tube; 11-a solvent; 12-bacterial biofilm.
Detailed Description
In order to make the technical solution of the present invention better understood, the technical solution of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts shall belong to the protection scope of the present invention.
It should be noted that the terms "first," "second," and the like in the description and claims of the present invention and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the invention described herein are capable of operation in sequences other than those illustrated or otherwise described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
The present invention will be described in further detail with reference to the accompanying drawings:
an object of the utility model is to provide a bacterial biofilm on culture solution board trades gets device to solve among the prior art and make the biomembrane easily flow through the washing liquid in trading the bacterial biofilm, the biomembrane is damaged easily and trade the long technical problem of time.
Specifically, as shown in fig. 1 and 2, the bacterial biofilm exchange device includes a bacterial biofilm culturing framework plate 1 disposed in a culture solution plate 8; the bacterial biofilm culture framework plate 1 comprises a plurality of groups of bacterial biofilm culture plates which are spliced in parallel; each group of bacterial biofilm culture plates comprises a plurality of bacterial biofilm culture blocks 4, a bacterial biofilm culture cup 6 is arranged in each bacterial biofilm culture block 4, the bacterial biofilms 12 are attached to the inner wall of each bacterial biofilm culture cup 6, and each bacterial biofilm culture cup 6 is placed in a solution cup of each culture solution plate 8.
Specifically, as shown in fig. 2, two sets of fixing buckles 2 are arranged on a bacterial biofilm culturing framework plate 1, one set of fixing buckles 2 is fixed on a first set of bacterial biofilm culturing strip-shaped plate of the bacterial biofilm culturing framework plate 1, the other set of fixing buckles 2 is fixed on a last set of bacterial biofilm culturing strip-shaped plate of the bacterial biofilm culturing framework plate 1, and the fixing buckles 2 are fixedly connected in clamping grooves on two sides of a culture solution plate 8.
Concretely, fixed buckle 2 is V type structure, and 2 one end of fixed buckle are 75 contained angles with the bacterial biofilm culture bar shaped plate and are connected, and the other end is the level setting, bottom length 5mm.
Specifically, as shown in FIG. 3, the two sides of the bacterial biofilm culturing strip-shaped plate are respectively provided with a replacing support handle 3, the length of the replacing support handle is 10mm, and the length of the replacing support handle is 5mm.
Specifically, folding indentations 5 are arranged between each group of bacterial biofilm culture strip-shaped plates and between each group of bacterial biofilm culture blocks 4, and the folding indentations 5 are distributed in a dotted line and used for independently disassembling the bacterial biofilm culture blocks 4.
Specifically, as shown in fig. 3, a lifting belt 7 is provided on one side of each bacterial biofilm culture block 4 for lifting the individual bacterial biofilm culture blocks 4.
Specifically, as shown in FIG. 4, the bacterial biofilm culturing cup 6 is a semi-permeable membrane truncated cone structure, wherein the inner diameter of each hole is 6.90mm, the height of each hole is 10.9mm, and the material is polycarbonate membrane.
Specifically, the number of the bacterial biofilm culture cups 6 in the bacterial biofilm culture framework plate 1 corresponds to the number of the solution cups in the culture solution plate 8, wherein the size of the bacterial biofilm culture framework plate 1 is 128mm × 86mm.
Example 1
According to fig. 5, in this embodiment, a biofilm bacteria culture framework plate 1 is provided, the biofilm bacteria culture framework plate 1 corresponds to a solution cup of a culture solution plate 8, a biofilm bacteria culture cup 6 is simultaneously placed in the solution cup of the culture solution plate 8 added with a culture medium/PBS, a fixing device of a fixing buckle 2 is used, when the culture solution/cleaning planktonic cells need to be replaced, the device is directly taken out, a new culture solution plate 8 is placed in, and the culture solution plate 8 is a 96-well plate.
Example 2
According to fig. 6, in the present embodiment, a bacterial biofilm culture framework plate is provided, after the bacterial biofilm transcriptomics research and the bacterial biofilm proteomics research, after the culture is completed, the individual bacterial biofilm culture blocks 4 can be obtained by individually disassembling each group of bacterial biofilm culture plates in the bacterial biofilm culture framework plate and each bacterial biofilm culture block 4 through the folding impression 5, the individual bacterial biofilm culture blocks 4 are placed in the EP tube 10 added with the required solvent 11, and the device is taken out of the EP tube 10 by using the lifting belt 7.
The utility model discloses an operating principle can dismantle the mode in the cultivation hole of taking out through adding the one deck, greatly reduces the biomembrane that loses because of uncontrollable factor in the experiment, will show the error that reduces in the relevant experiment of biomembrane. And due to the design of convenient disassembly and replacement, the time required by the experiment is greatly shortened, and the operation difficulty is reduced. In order to reduce the possibility of pollution of the old experimental method, the device reduces the possibility of pollution through the disassembly type and the lifting belt.
To sum up, the utility model discloses this bacterial biofilm trades device has simplified the relevant experiment of biomembrane greatly, the utility model discloses can accomplish the experiment in the aspect of the little biological characteristic in the biomembrane research, observe, biomembrane transcriptomics and proteomics analysis and determination including the electron microscope of biomembrane formation ability quantitative determination (micropore board crystal violet dyeing method), the micro-form of biomembrane. The method specifically comprises the following three aspects:
in the biofilm formation ability quantitative detection experiment, the culture wells with required number can be directly taken down through the folding and pressing marks, inserted into a common 96-well plate and fixed on the plate, so that the experiment operation is convenient; the required liquid can be directly added into a new 96-pore plate in the cleaning and liquid changing operations, and then the device is taken down and loaded into the new 96-pore plate, so that the operation time is greatly shortened, the operation difficulty is reduced, the retention rate of the biological membrane is improved, and the device is suitable for measuring the biological membrane with high flux.
In a confocal microscope observation experiment of a biological membrane microscopic form, a single hole after culture is taken down, washed by PBS and directly installed on an installation medium, and operations such as dyeing, fixing and the like are performed. Compared with the glass slide method of the existing scheme, the device has the advantages that the biological membrane only grows on the polycarbonate membrane, the surrounding non-hole positions are convenient to clamp and do not need to worry about damaging the integrity of the organism, the operation difficulty is reduced, and the experiment duration is shortened.
In the research of bacterial biomembrane transcriptomics and proteomics, the culture hole after the culture is finished is taken down and is placed into an EP tube, and the lifting belt is left outside the EP tube. The biomembrane is peeled off through operations such as vortex oscillation, and after peeling is finished, the device is directly taken out by using the lifting belt, so that a feasibility scheme for liquid culture biomembranes is provided, the method is suitable for extracting biomembrane samples by a high-flux method, and is simple and convenient to operate and not easy to pollute.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit the same, and although the present invention is described in detail with reference to the above embodiments, those of ordinary skill in the art should understand that: modifications and equivalents of the embodiments of the invention may be made without departing from the spirit and scope of the invention, which should be construed as falling within the scope of the claims of the invention.
Claims (10)
1. A bacterial biofilm replacing device on a culture solution plate is characterized by comprising a bacterial biofilm culturing framework plate (1); the bacterial biofilm culturing framework plate (1) comprises a plurality of groups of bacterial biofilm culturing strip-shaped plates which are spliced in parallel; each group of bacterial biofilm culturing strip-shaped plates comprise a plurality of bacterial biofilm culturing blocks (4), a bacterial biofilm culturing cup (6) is arranged in each bacterial biofilm culturing block (4), a bacterial biofilm (12) is attached to the inner wall of each bacterial biofilm culturing cup (6), each bacterial biofilm culturing cup (6) is placed in a solution cup of each culture solution plate (8), and a solution in each solution cup is in contact with the bacterial biofilm (12) through each bacterial biofilm culturing cup (6).
2. The device for exchanging the bacterial biofilm on the culture solution plate as claimed in claim 1, wherein two sets of fixing buckles (2) are arranged on the bacterial biofilm culturing framework plate (1), one set of fixing buckles (2) is fixed on a first set of bacterial biofilm culturing strip-shaped plates of the bacterial biofilm culturing framework plate (1), the other set of fixing buckles (2) is fixed on a last set of bacterial biofilm culturing strip-shaped plates of the bacterial biofilm culturing framework plate (1), and the fixing buckles (2) are fixedly connected in clamping grooves on two sides of the culture solution plate (8).
3. The device for replacing the bacterial biofilm on the culture solution plate as claimed in claim 2, wherein the fixing buckle (2) is of a V-shaped structure, one end of the fixing buckle (2) is connected with the bacterial biofilm culture strip-shaped plate at an included angle of 75 degrees, and the other end of the fixing buckle is horizontally arranged.
4. The device for replacing bacterial biofilms on culture solution plates according to claim 1, wherein the two sides of the bacterial biofilm culture strip plates are respectively provided with a replacing support handle (3).
5. The device for exchanging bacterial biofilms on culture solution plates, according to claim 1, wherein folding indentations (5) are provided between each group of bacterial biofilm culture strips and between each bacterial biofilm culture block (4), said folding indentations (5) being distributed in a dotted line for individually disassembling the bacterial biofilm culture blocks (4).
6. A bacterial biofilm exchange device on a culture plate according to claim 1, wherein each bacterial biofilm culture block (4) is provided with a lifting belt (7) at one side for lifting the single bacterial biofilm culture block (4).
7. The device for replacing bacterial biofilm on the culture solution plate as claimed in claim 1, wherein the bacterial biofilm culture cup (6) is of a semi-permeable membrane truncated cone structure, and a polycarbonate membrane is adopted as a material.
8. A bacterial biofilm exchange device on a culture plate according to claim 1, wherein said bacterial biofilm culturing cup (6) is located at the central position of the bacterial biofilm culturing block (4).
9. The device for exchanging bacterial biofilm on culture plates as claimed in claim 1, wherein the number of bacterial biofilm culture cups (6) in the bacterial biofilm culture framework plate (1) corresponds to the number of solution cups of the culture plate (8).
10. The device for exchanging bacterial biofilm on culture plates as claimed in claim 1, wherein the cup diameter of the solution cup of the culture plate (8) is smaller than the plate diameter of the bacterial biofilm culture block (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223200223.1U CN218879896U (en) | 2022-11-29 | 2022-11-29 | Bacterial biofilm on culture solution plate trades gets device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223200223.1U CN218879896U (en) | 2022-11-29 | 2022-11-29 | Bacterial biofilm on culture solution plate trades gets device |
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| Publication Number | Publication Date |
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| CN218879896U true CN218879896U (en) | 2023-04-18 |
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| CN202223200223.1U Active CN218879896U (en) | 2022-11-29 | 2022-11-29 | Bacterial biofilm on culture solution plate trades gets device |
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- 2022-11-29 CN CN202223200223.1U patent/CN218879896U/en active Active
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