CN218872244U

CN218872244U - Novel detection reagent disc

Info

Publication number: CN218872244U
Application number: CN202220126032.6U
Authority: CN
Inventors: 吴卫阳
Original assignee: XIAMEN YUNPENG SCIENCE AND TECHNOLOGY DEVELOPMENTCO Ltd
Current assignee: XIAMEN YUNPENG SCIENCE AND TECHNOLOGY DEVELOPMENTCO Ltd
Priority date: 2022-01-18
Filing date: 2022-01-18
Publication date: 2023-04-18
Anticipated expiration: 2032-01-18

Abstract

The utility model discloses a novel detect reagent disc relates to detect reagent disc technical field, can not adjust the chip quantity that uses when aiming at solving current micro-fluidic disc and using, easily cause the waste of chip, improve the detection cost, the problem of extravagant resource, the utility model discloses a set up the chip groove of annular array on the disc, set up constant head tank and socket in the chip inslot, make the chip fix in the chip inslot very easily to can nimble quantity of chip on the adjustment disc, use the chip of different quantity to detect as required, reach the maximize of chip quantity, carry out make full use of to the chip, reduce the waste of chip, practice thrift the cost, through setting up a plurality of trench on the chip, utilize the different rotational speeds of detector, can control solution and the test agent that awaits measuring on the chip, thereby the going on of control detection reaction, through the corotation of disc, reversal, shock mode such as, accelerated reaction rate, improved detection efficiency, shortened check-out time.

Description

Novel detection reagent disc

Technical Field

The utility model relates to a detect reagent disc technical field, specifically be a novel detect reagent disc.

Background

The microfluidic disc serving as a novel analysis and detection platform has the advantages of high flux, integration, portability, easiness in operation, low cost and the like, and is widely applied to various fields.

The micro-fluidic analysis chip is a main platform for realizing the micro-fluidic technology (Mixrofluiyixs), and can integrate basic operation units of sample preparation, reaction, separation, detection and the like in the biological, chemical and medical analysis process on a micron-scale chip to automatically finish the whole analysis process.

The micro-fluidic disc adopts micro-electro-mechanical processing technology similar to a semiconductor to construct a micro-channel system on a chip, transfers the experiment and analysis process to a chip structure consisting of a path and a small liquid-phase chamber which are mutually connected, and drives the flow of buffer solution in the chip by adopting methods such as a micro-mechanical pump, an electric hydraulic pump, electroosmotic flow and the like after loading a biological sample and reaction liquid to form a micro-channel, so as to carry out one or more continuous reactions on the chip.

The existing microfluidic disk adopts a production mode of integral production and manufacturing, the number of chips used cannot be adjusted when the existing microfluidic disk is used, the waste of the chips is easily caused, the detection cost is increased, and the resources are wasted.

SUMMERY OF THE UTILITY MODEL

In view of the problem that exists among the prior art, the utility model discloses a novel detect reagent disc, the technical scheme of adoption is, including the disc, the disc is circular disk body, be equipped with chip groove, mounting hole on the disc, the centre of a circle department of disc is equipped with the mounting hole, the chip groove have the multiunit and use the centre of a circle forms annular array as the center, be close to on the chip groove the one end in the centre of a circle is equipped with the constant head tank, the both sides in chip groove are equipped with the socket, the socket has multiunit and symmetry to set up, the below of socket is equipped with the spout, the spout with the size phase-match of socket, sliding connection has the chip in the chip groove, be equipped with locating piece, slider on the chip, the locating piece with constant head tank size phase-match position is corresponding, the slider with the size phase-match position of socket is corresponding, the slider with the size phase-match of spout.

As a preferred technical scheme of the utility model, the chip is equipped with application of sample groove, separating tank, ration groove, raffinate groove, injection tank I, injection tank II, reaction tank, waste liquid groove, capillary channel has the multiunit, application of sample groove is close to the locating piece, application of sample groove with the left side of separating tank is passed through capillary channel connects, the right side of separating tank is equipped with separation channel, separation channel's middle section is through annotating liquid channel connection ration groove, separation channel's end is equipped with raffinate groove, ration groove passes through the miniflow valve and connects the reaction tank, the right side of reaction tank is passed through capillary channel connects injection tank I injection tank II, the left side of reaction tank is passed through capillary channel connects the waste liquid groove can be under the centrifugal force effect of difference, the control solution that awaits measuring reacts with the test agent going on.

As the utility model discloses a preferred technical scheme, be equipped with the sample loading port on the sample loading groove, the injection groove I all be equipped with the filling opening on the injection groove II, be equipped with the gas pocket on the waste liquid groove, the sample loading port can be used to the dropwise add solution that awaits measuring, the filling opening is used for dropwise add test agent, the gas pocket is used for the exhaust air.

As a preferred technical scheme of the utility model, the constant head tank with the locating piece all is equipped with two sets ofly, the constant head tank is the dovetail groove, can be convenient for the locating piece inserts in the constant head tank.

As a preferred technical scheme of the utility model, separation channel the notes liquid passageway all reaches hydrophobic effect through the infiltration nature that changes the passageway inner wall through hydrophobic processing.

As an optimized technical solution of the present invention, the number of the chip grooves is an integral multiple of 3, and the chip grooves can form an annular array.

As an optimal technical scheme of the utility model, the injection groove I with between the reaction tank capillary passageway with the contained angle of reaction tank is the acute angle, injection groove II with the contained angle of reaction tank is the right angle, and is usable the angle of capillary passageway is different, makes it reach predetermined filling requirement under different rotational speeds.

As an optimal technical scheme of the utility model, the injection tank I with be equipped with vice injection tank between the reaction tank, can add other functional reagent as required, reach multiple detection effect.

The utility model has the advantages that: the utility model discloses a set up the chip groove of annular array on the disc, set up constant head tank and socket in the chip inslot, make the chip fix at the chip inslot very easily, thereby can adjust the quantity of chip on the disc in a flexible way, the chip that uses different quantity as required detects, reach the maximize of chip quantity, carry out make full use of to the chip, reduce the waste of chip, save the cost, through setting up a plurality of trench on the chip, utilize the different rotational speeds of detector, can control solution that awaits measuring and test agent on the chip, thereby the going on of control detection reaction, through the corotation of disc, the reversal, mode such as shock, reaction rate has been accelerated, detection efficiency is improved, detection time has been shortened.

Drawings

The technical solution of the present invention will be clearly and completely described with reference to the accompanying drawings. In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below; throughout the drawings, like elements or portions are generally identified by like reference numerals; in the drawings, elements or portions are not necessarily drawn to scale.

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural diagram of a disk of the present invention;

FIG. 3 is a schematic view of a chip groove structure according to the present invention;

fig. 4 is a schematic diagram of the chip structure of the present invention.

In the figure: 1. a disk; 2. a chip slot; 3. mounting holes; 4. a chip; 21. positioning a groove; 22. a socket; 23. a chute; 41. positioning blocks; 42. a slider; 501. a sample adding slot; 502. a sample addition port; 503. a separation tank; 504. a separation channel; 505. a quantifying tank; 506. a residual liquid tank; 507. a microfluidic valve; 508. injecting into a groove I; 509. an injection groove II; 510. a reaction tank; 511. a waste liquid tank.

Detailed Description

Example 1

As shown in fig. 1-4, the utility model discloses a novel detect reagent disc, the technical scheme of adoption is, including disc 1, disc 1 is circular disk body, disc 1 is last to be equipped with chip groove 2, mounting hole 3, the centre of a circle department of disc 1 is equipped with mounting hole 3, chip groove 2 have the multiunit and use the centre of a circle is annular array for the center, be close to on the chip groove 2 the one end in the centre of a circle is equipped with constant head tank 21, the both sides in chip groove 2 are equipped with socket 22, socket 22 has multiunit and symmetry to set up, the below of socket 22 is equipped with spout 23, spout 23 with the size phase-match of socket 22, sliding connection has chip 4 in the chip groove 2, be equipped with locating piece 41, slider 42 on the chip 4, locating piece 41 with constant head tank 21 size phase-match position is corresponding, slider 42 with socket 22's size phase-match position is corresponding, slider 42 with the size phase-match of spout 23.

As a preferred technical scheme of the utility model, chip 4 is equipped with application of sample groove 501, separation tank 503, ration groove 505, surplus liquid groove 506, injection tank I508, injection tank II 509, reaction tank 510, waste liquid groove 511, capillary channel has the multiunit, application of sample groove 501 is close to locating piece 41, application of sample groove 501 with the left side of separation tank 503 is passed through capillary channel connects, separation tank 503's right side is equipped with separation channel 504, separation channel 504's middle section is through annotating liquid channel connection ration groove 505, separation channel 504's end is equipped with surplus liquid groove 506, ration groove 505 passes through miniflow valve 507 and connects reaction tank 510, reaction tank 510's right side is passed through capillary channel connects injection tank I508, injection tank II 509, reaction tank 510's left side is passed through capillary channel connects waste liquid groove 511 can be under the centrifugal force effect of difference, and the solution that the control awaits measuring is reacted with the reagent goes on, improves the reaction precision, improves detection efficiency.

As the utility model discloses a preferred technical scheme, be equipped with the sample loading port 502 on the sample loading slot 501, the injection tank I508 all be equipped with the filling opening on the injection tank II 509, be equipped with the gas pocket on the waste liquid groove 511, the sample loading port 502 can be used to the dropwise add solution that awaits measuring, the filling opening is used for dropwise add test agent, the gas pocket is used for the exhaust air, improves the accuracy that detects.

As a preferred technical scheme of the utility model, constant head tank 21 with locating piece 41 all is equipped with two sets ofly, constant head tank 21 is the dovetail groove, can be convenient for locating piece 41 inserts in the constant head tank 21, improve installation effectiveness, save time.

As a preferred technical scheme of the utility model, separation channel 504 the filling channel all reaches hydrophobic effect through the infiltration nature that changes the passageway inner wall through hydrophobic processing, cuts off the both sides solution that awaits measuring.

As an optimized technical scheme of the utility model, the quantity of chip groove 2 is 6, chip groove 2 can form annular array, strengthens stability when 1 high-speed rotations of disc.

As a preferred technical scheme of the utility model, injection groove I508 with between the reaction tank 510 capillary passageway with the contained angle of reaction tank 510 is the acute angle, injection groove II 509 with the contained angle of reaction tank 510 is the right angle, and is usable the angle of capillary passageway is different, makes it reach predetermined filling requirement under different rotational speeds.

As a preferred technical scheme of the utility model, the input tank I508 with be equipped with vice input tank between the reaction tank 510, can add other functional reagent as required, reach multiple detection effect, strengthen chip 4's functionality.

The utility model discloses a theory of operation: when in use, the mounting holes 3 are arranged on a rotating shaft of a detector, the chip 4 to be detected is placed on the disk 1, the chip 4 is arranged in an annular array and is uniformly arranged, the chip 4 is placed in the chip groove 2, firstly, the positioning block 41 is aligned with the positioning groove 21 and is placed, then, the chip 4 is placed in the chip groove 2, the slide block 42 enters the slide groove 23 from the insertion opening 22, the chip 4 is shifted to the outer side of the disk 1, the slide block 42 is clamped in the slide groove 23, the chip 4 is limited in the chip groove 2, the liquid to be detected is dripped from the sample injection opening 502 to enter the sample injection groove 501, the testing agent is dripped from the injection opening to enter the injection groove I508 and the injection groove II 509, then, the detector is started, the rotating shaft drives the disk 1 to rotate, thereby driving the chip 4 to rotate, centrifugal detection is carried out, different centrifugal forces are generated by utilizing different rotating speeds of the detector in the centrifugal detection process, thereby controlling the liquid to be detected and the testing agent on the chip 4, finally, the purpose of controlling the reaction is achieved, under the condition of a rotating speed a, the component x and the component y in the liquid to be detected are separated, the component x and the component y both enter a separation groove 503, the component x is left in the separation groove 503, the component y continuously flows into a separation channel 504 and then enters a quantitative groove 505, the component y cannot break through a micro-flow valve 507 under the rotating speed a, after the quantitative groove 505 overflows, the component y flows into a residual liquid groove 506 along the separation channel 504, the rotating speed is increased to reach a rotating speed b, the centrifugal force received by the component y in the quantitative groove 505 is increased, the component y breaks through the micro-flow valve 507 and enters a reaction groove 510, meanwhile, the test agent in an injection groove I508 enters the reaction groove 510 along an inclined capillary channel to generate a first step reaction, when a detector reaches the rotating speed c, the test agent in an injection groove II 509 enters the reaction groove 510 along the capillary channel, the detector oscillates to make the reaction in the reaction tank 510 more complete, the excess solution to be tested and the test agent enter the waste liquid tank 511, and after the reaction is finished, the disk 1 can be taken out for further observation.

The mechanical connection of the present invention is a conventional means used by those skilled in the art, and can be suggested by a limited number of tests, which belong to the common knowledge.

Components not described in detail herein are prior art.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; the two elements may be directly connected or indirectly connected through an intermediate medium, and the two elements may be connected through an intermediate medium.

Although the present invention has been described in detail with reference to the specific embodiments, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge range of those skilled in the art, and modifications or variations without creative efforts are still within the scope of the present invention.

Claims

1. A novel assay reagent disk comprising a disk (1) characterized in that: disc (1) is circular disk body, be equipped with chip groove (2), mounting hole (3) on disc (1), the centre of a circle department of disc (1) is equipped with mounting hole (3), chip groove (2) have the multiunit and with the centre of a circle is annular array for the center, be close to on chip groove (2) the one end in the centre of a circle is equipped with constant head tank (21), the both sides of chip groove (2) are equipped with socket (22), socket (22) have multiunit and symmetry to set up, the below of socket (22) is equipped with spout (23), spout (23) with the size phase-match of socket (22), sliding connection has chip (4) in chip groove (2), be equipped with locating piece (41), slider (42) on chip (4), locating piece (41) with constant head tank (21) size phase-match position is corresponding, slider (42) with the size phase-match position of socket (22) is corresponding, slider (42) with the size phase-match of spout (23).

2. The novel detector reagent disk of claim 1, wherein: chip (4) are equipped with application of sample groove (501), separating tank (503), ration groove (505), surplus liquid groove (506), injection tank I (508), injection tank II (509), reaction tank (510), waste liquid groove (511), capillary channel has the multiunit, application of sample groove (501) are close to locating piece (41), application of sample groove (501) with the left side of separating tank (503) is passed through capillary channel connects, the right side of separating tank (503) is equipped with separation channel (504), the middle section of separation channel (504) is through annotating liquid channel connection ration groove (505), the end of separation channel (504) is equipped with surplus liquid groove (506), ration groove (505) are connected through miniflow valve (507) reaction tank (510), the right side of reaction tank (510) is passed through capillary channel connects injection tank I (508), injection tank II (509), the left side of reaction tank (510) is passed through capillary channel connects waste liquid groove (511).

3. The novel detector reagent disk of claim 2, wherein: and a sample adding port (502) is formed in the sample adding groove (501), injection ports are formed in the injection groove I (508) and the injection groove II (509), and air holes are formed in the waste liquid groove (511).

4. The novel detector reagent disk of claim 1 wherein: the positioning grooves (21) and the positioning blocks (41) are both provided with two groups, and the positioning grooves (21) are trapezoidal grooves.

5. The novel detector reagent disk of claim 2 wherein: the separation channel (504) and the liquid injection channel are both subjected to hydrophobic treatment.

6. The novel detector reagent disk of claim 1 wherein: the number of the chip grooves (2) is a multiple of three.

7. The novel detector reagent disk of claim 2, wherein: the included angle between the capillary channel and the reaction tank (510) between the injection groove I (508) and the reaction tank (510) is an acute angle, and the included angle between the injection groove II (509) and the reaction tank (510) is a right angle.

8. The novel detector reagent disk of claim 7 wherein: an auxiliary injection groove is arranged between the injection groove I (508) and the reaction groove (510).