CN218865926U - Cylindrical inspection chip for cat plague - Google Patents
Cylindrical inspection chip for cat plague Download PDFInfo
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- CN218865926U CN218865926U CN202222253654.8U CN202222253654U CN218865926U CN 218865926 U CN218865926 U CN 218865926U CN 202222253654 U CN202222253654 U CN 202222253654U CN 218865926 U CN218865926 U CN 218865926U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The utility model provides a cylindrical inspection chip of cat plague, including the chip carrier, chip carrier top is equipped with the sampling mouth, is equipped with filtration membrane in the sampling mouth, and the filtration membrane below is equipped with first microchannel, and first microchannel below is equipped with the reaction tank, and the reaction tank below is equipped with second microchannel, and second microchannel below is equipped with the dashpot, and the dashpot below is equipped with third microchannel, and third microchannel is connected with detection channel, and the detection channel below is equipped with fourth microchannel. The utility model discloses it is convenient to detect, and detection efficiency is high, conveniently carries.
Description
Technical Field
The utility model relates to a cat plague virus detection device technical field, in particular to cylindrical inspection chip of cat plague.
Background
The feline infectious diseases, also called feline panleukopenia and feline infectious enteritis, are acute high-contact infectious diseases of cats, the morbidity and mortality are high, and recovered animals can carry viruses for a long time to become potential hidden dangers, so that the feline infectious diseases virus needs to be quickly detected.
SUMMERY OF THE UTILITY MODEL
The object of the utility model is to solve at least one of the technical defects.
Therefore, an object of the present invention is to provide a cylindrical inspection chip for cat plague, so as to solve the problems mentioned in the background art and overcome the disadvantages existing in the prior art.
In order to realize the above-mentioned purpose, the embodiment of the utility model provides a cylindrical inspection chip of cat plague, including the chip carrier, chip carrier top is equipped with the sample connection, is equipped with filtration membrane in the sample connection, and the filtration membrane below is equipped with first microchannel, and first microchannel below is equipped with the reaction tank, and the reaction tank below is equipped with the second microchannel, and the second microchannel below is equipped with the dashpot, and the dashpot below is equipped with the third microchannel, and the third microchannel is connected with detecting channel, and detecting channel below is equipped with the fourth microchannel.
Preferably, the detection channel consists of a cylindrical matrix.
In any of the above embodiments, preferably, the upper end of the detection channel is attached with an anti-feline pestivirus antibody, and the lower end of the detection channel is attached with an anti-murine IgG antibody.
In any of the above embodiments, the sampling port preferably has a conical bottom.
In any of the above embodiments, preferably, a waste liquid tank is provided below the fourth microchannel.
In any of the above aspects, it is preferable that the number of the second microchannels is two.
In any of the above aspects, it is preferable that the number of the fourth microchannels is two.
In any of the above embodiments, the reaction tank is preferably filled with an anti-feline pestivirus antibody-binding copolymer labeled with red fluorescent microspheres.
In any of the above embodiments, it is preferable that the chip carrier is a cylinder, the length of the chip carrier is 70mm, and the diameter of the chip carrier is 3.5mm.
In any of the above schemes, preferably, the chip carrier and the detection channel are both made of PDMS material.
Compared with the prior art, the utility model has the advantages and beneficial effects do:
1. the utility model discloses an embedded miniature passageway of cat plague cylindrical inspection chip, dashpot, reaction tank, detection line passageway etc. more convenient in the testing process prevents the interference of external factor to the detection sample in the testing process, only need instil into the sample and arrive the sample connection, utilizes the gravity of sample self to pass through miniature passageway and detection channel, can accomplish the detection, and detection efficiency is high, and whole journey need not the manual work and carries out other operations, and is more convenient.
2. The utility model discloses a be equipped with filtration membrane in the cylindrical inspection chip sampling mouth of cat plague, filter the impurity in the sample, make more pure of detected sample, detect more accurate.
3. The utility model discloses a cylindrical inspection chip of cat plague conveniently carries, requires lowly to the detection ring border, detects convenience more.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
fig. 1 is a front view of a cylindrical inspection chip for feline infectious diseases according to the embodiment of the present invention.
Fig. 2 is a left side view of the cylindrical inspection chip for cat plague of the present invention shown in fig. 1.
Fig. 3 is a schematic diagram of a positive detection result of the cylindrical chip for detecting feline panleukopenia according to the embodiment of the present invention.
Fig. 4 is a schematic diagram of a negative detection result of the cylindrical chip for detecting feline panleukopenia according to the embodiment of the present invention.
Fig. 5 is a schematic diagram of the invalid detection result of the cylindrical inspection chip for cat plague according to the embodiment of the present invention.
Wherein: 1-a chip carrier; 2-a sampling port; 3-a filtration membrane; 4-a first microchannel; 5-a reaction tank; 6-a second microchannel; 7-a buffer tank; 8-a third microchannel; 9-detection channel; 10-a fourth microchannel; 11-a waste liquid tank; 12-T line detection line; and 13-C linear quality control detection line.
Detailed Description
Reference will now be made in detail to the embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary intended for explaining the present invention, and should not be construed as limiting the present invention.
In the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly, e.g., as being fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those of ordinary skill in the art.
As shown in fig. 1 and fig. 2, the utility model discloses a cylindrical inspection chip of cat plague, including chip carrier 1, 1 top of chip carrier is equipped with sampling port 2, is equipped with filtration membrane 3 in the sampling port 2, and filtration membrane 3 below is equipped with first micro channel 4, and first micro channel 4 below is equipped with reaction tank 5, and reaction tank 5 below is equipped with second micro channel 6, and second micro channel 6 below is equipped with dashpot 7, and the dashpot 7 below is equipped with third micro channel 8, and third micro channel 8 is connected with sense passage 9, and sense passage 9 below is equipped with fourth micro channel 10. The chip carrier 1 is a chip housing.
The chip carrier 1 of the utility model is formed by die casting and bonding a grinding tool, and a circular groove and a micro channel are formed in the chip; the top of the sampling tube is provided with a circular sampling port 2, a layer of filter membrane is arranged at the sampling port 2 and used for filtering a sample, and the lower end of the sampling port 2 is conical and is connected with a first micro channel 4; the micro channel is connected with a circular reaction tank 5, and the bottom of the reaction tank 5 contains an anti-feline pestivirus antibody binding copolymer marked with red fluorescent microspheres; the bottom of the reaction tank 5 is connected with a second micro-channel 6; the bottom of the second micro-channel 6 is connected with the buffer groove 7, pressure is formed by gravity, and liquid can flow into the detection channel 9 through the buffer groove 7; the surface of the detection channel 9 is composed of a cylindrical matrix, after treatment, the upper end is attached with a matched anti-feline plague virus antibody to form a strip shape, and the lower end is attached with a coating anti-mouse IgG antibody to form a strip shape. The bottom of the detection channel 9 is provided with a fourth micro-channel 10, and the lower part of the detection channel is connected with a waste liquid groove 11; the chip carrier 1 is a cylinder, when in use, the chip carrier is vertically arranged on a table top, and the liquid sample forms pressure and flow velocity by means of the gravity of the liquid sample and traverses the whole detection chip. The top end of the sampling port 2 is provided with a cylindrical sampling port 2, the middle part of the sampling port is provided with a layer of filter membrane for filtering a sample, large-particle impurities are removed, the micro channel is prevented from being blocked, and the bottom of the sampling port is conical and is connected with the micro channel.
The utility model discloses a new red fluorescence microballon and antigen or antibody carry out condensation reaction, form irreversible whole, have realized the mark to target protein to prepare out cat plague virus and detect the chip. The rhodamine B fluorescent dye and the modified styrene are copolymerized together to prepare the fluorescent microsphere, and the surface hydroxyl functional groups can be synthesized with the target protein into a whole through condensation reaction to finally form the protein for substituting red fluorescence.
Further, the detection channel 9 is composed of a cylindrical matrix. The cylinder matrix includes a plurality of cylinders of arranging along vertical direction, and every cylinder is hollow structure, and the structure of similar honeycomb when detecting liquid flow through the cylinder flows through hollow cylinder structure, sets up hollow cylinder structure and conveniently paints cat plague virus antibody and anti mouse source IgG antibody, reacts with antigen and antibody when detecting liquid flow through hollow cylinder, makes things convenient for the later stage to observe.
Furthermore, an anti-feline pestivirus antibody is attached to the upper end of the detection channel 9, and an anti-murine IgG antibody is attached to the lower end of the detection channel 9.
Alternatively, the bottom of the sampling port 2 is formed in a conical shape. Facilitating the flow of sample liquid into the first microchannel 4.
Further, a waste liquid tank 11 is provided below the fourth microchannel 10. The waste liquid is recovered by the waste liquid tank 11, preventing contamination.
Specifically, the second microchannel 6 is provided in two. The two second microchannels 6 are parallel to each other.
Further, the fourth micro channel 10 is provided in two. The two fourth microchannels 10 are parallel to each other.
Specifically, the reaction tank 5 is filled with an anti-feline pestivirus antibody binding copolymer marked with red fluorescent microspheres. The first microchannel 4 is connected with the reaction tank 5, and the reaction tank 5 contains an anti-feline fever virus antibody binding copolymer marked with red fluorescent microspheres. 150ul of liquid sample is put in through the sampling port 2, filtered by the filter membrane 3, enters the reaction tank 5 along the first micro-channel 4 under the self-gravity regulation, and is mixed with the antibody marked with the red fluorescent microspheres. The mixture of the sample and the antibody marked with the red fluorescent microspheres flows through two parallel second microchannels 6, enters a buffer tank 7, then enters a detection channel 9, and finally flows into a waste liquid tank 11.
Specifically, the chip carrier 1 is a cylinder, the length of the chip carrier 1 is 70mm, and the diameter of the chip carrier 1 is 2mm. Optionally, the chip carrier 1 and the detection channel 9 are both made of PDMS (polydimethylsiloxane) materials, and the whole is transparent and visible.
The utility model discloses an operation flow:
1. and uniformly spraying the solution containing the marked feline infectious bronchitis virus antibody and the red fluorescent microsphere polymer onto the reaction tank 5 according to a certain proportion.
2. The paired antibodies of the feline panleukosis virus are coated on the corresponding positions at the upper end of the detection channel 9 according to a certain proportion and are coated into a strip shape.
3. And (3) coating the anti-mouse IgG antibody on the corresponding position at the lower end of the detection channel 9 according to a certain proportion, and coating the anti-mouse IgG antibody into a strip shape.
4. After the treatment, the chips were oven dried for 24 hours (temperature 40 ℃).
5. And then bonding and pressing the chip, standing for 3 hours in an environment with the temperature of 4 ℃, solidifying the glue, and then testing.
The utility model discloses a cylindrical inspection chip theory of operation of cat plague does:
put chip carrier 1 perpendicularly, will await measuring the sample and drip into through sampling port 2, get rid of large granule impurity through the filtration of filter membrane 3, prevent that the later stage from blockking up miniature passageway, in the sample enters into first miniature passageway 4 through the bottom cone, first miniature passageway 4 and reaction tank 5 intercommunication to pass and enter into reaction tank 5, enter into in reaction tank 5 and mix with the antibody that the mark is good, form canine cat pestivirus and antibody fluorescence microsphere polymer. Then the sample enters a buffer groove 7 through a second micro channel 6 and continuously enters a detection channel 9, a T line detection line is a long line coated with a feline pestivirus antibody on the detection channel 9, the T line detection line and a paired feline pestivirus antibody coated on the T line detection line carry out sandwich reaction, if the sample contains the feline pestivirus, a fluorescent compound of a labeled antibody combined antigen and the paired antibody (sandwich method) is formed on the T line detection line, and red fluorescence is deposited on the fluorescent compound, so that the T line detection line can display red fluorescence. If the sample does not contain feline pestivirus, the T-line detection line cannot display red fluorescence. The C line quality control detection line is a long line coated with IgG antibodies on the detection channel 9, meanwhile, the mouse source antibody fluorescent microsphere polymer in the sample can be combined with the anti-mouse source IgG antibodies coated on the C line quality control detection line to form a red fluorescent compound, and the C line quality control detection line can display red fluorescence. The sample flows out of the detection channel 9 and is finally absorbed and recovered completely after entering the waste liquid 8. The red fluorescence value can be observed and quantitatively detected by a related fluorescence quantitative detection instrument, and the obvious red fluorescence is displayed under the irradiation of 532nm excitation wavelength.
And (4) judging a result:
as shown in fig. 3, fig. 3 shows:
and (3) displaying strips on the T line detection line and the C line quality control detection line under the irradiation of a fluorescent lamp, and proving that the sample contains the feline infectious disease virus. Note that: the fluorescence detection instrument can be used for observing the strength of fluorescence coloration of the T-line detection line, so that the content of the feline panleukosis virus in the sample can be quantitatively analyzed.
As shown in FIG. 4, no strip was shown in the T-line test line and a strip was shown in the C-line quality control test line under the irradiation of fluorescent light, and as a negative result, the sample did not contain feline panleukosis virus
As shown in FIG. 5, the C-line quality control detection line shows no bands, and is considered as an invalid result, and needs to be re-detected.
The utility model discloses a cylindrical inspection chip of cat plague convenient to carry does not need artifical the participation in the testing process, detects convenient simple, has improved the detection efficiency of cat plague virus greatly.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the invention, and that those skilled in the art may make variations, modifications, substitutions and alterations herein without departing from the spirit and scope of the invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. The utility model provides a cylindrical inspection chip of cat plague, a serial communication port, includes the chip carrier, chip carrier top is equipped with the sample connection, be equipped with filtration membrane in the sample connection, the filtration membrane below is equipped with first micro channel, first micro channel below is equipped with the reaction tank, the reaction tank below is equipped with the micro channel of second, the micro channel below of second is equipped with the dashpot, the dashpot below is equipped with the third micro channel, the third micro channel is connected with the test channel, the test channel below is equipped with the fourth micro channel.
2. The cylindrical inspection chip for feline plague of claim 1, characterized in that the detection channel is composed of a cylindrical matrix.
3. The cylindrical detection chip for feline panleukopenia according to claim 2, wherein the upper end of the detection channel is attached with an anti-feline panleukopenia virus antibody, and the lower end of the detection channel is attached with an anti-murine IgG antibody.
4. The cylindrical chip for detecting feline bacterial wilt according to claim 1, wherein the bottom of the sampling port is conical.
5. The cylindrical inspection chip for feline plague according to claim 1, characterized in that a waste liquid tank is provided below the fourth microchannel.
6. The cylindrical inspection chip of claim 1, wherein there are two second microchannels.
7. The cylindrical inspection chip for feline bacterial wilt according to claim 1, wherein there are two of said fourth micro-channels.
8. The cylindrical chip for detecting feline fever according to claim 1, wherein the reaction tank is filled with an anti-feline fever virus antibody-binding copolymer labeled with red fluorescent microspheres.
9. The cylindrical inspection chip for feline bacterial wilt according to claim 1, wherein the chip carrier is a cylinder, the length of the chip carrier is 70mm, and the diameter of the chip carrier is 3.5mm.
10. The cylindrical chip for detecting feline bacterial wilt according to claim 1, wherein both the chip carrier and the detection channel are made from PDMS material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222253654.8U CN218865926U (en) | 2022-08-25 | 2022-08-25 | Cylindrical inspection chip for cat plague |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222253654.8U CN218865926U (en) | 2022-08-25 | 2022-08-25 | Cylindrical inspection chip for cat plague |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218865926U true CN218865926U (en) | 2023-04-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222253654.8U Active CN218865926U (en) | 2022-08-25 | 2022-08-25 | Cylindrical inspection chip for cat plague |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218865926U (en) |
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2022
- 2022-08-25 CN CN202222253654.8U patent/CN218865926U/en active Active
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