CN218860754U - Automatic cell culture workstation - Google Patents
Automatic cell culture workstation Download PDFInfo
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- CN218860754U CN218860754U CN202222034619.7U CN202222034619U CN218860754U CN 218860754 U CN218860754 U CN 218860754U CN 202222034619 U CN202222034619 U CN 202222034619U CN 218860754 U CN218860754 U CN 218860754U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
Abstract
The utility model relates to a cell culture technical field specifically provides an automatic change cell culture workstation, aims at solving current automatic cell culture workstation disinfection inefficiency and easily arouses cross contamination's problem. Mesh for this reason, the utility model discloses an automatic cell culture workstation includes the box, is equipped with the first cavity and the second cavity that are linked together in the box, still includes degassing unit, and degassing unit still includes degassing unit including the container that holds disinfectant, the injection apparatus towards first cavity and/or the inside injection disinfectant of second cavity, container and injection apparatus intercommunication still are equipped with first fan in the first cavity, still are equipped with the second fan in the second cavity, the air-out direction subtend setting of first fan and second fan.
Description
Technical Field
The utility model relates to a cell culture technical field specifically provides an automatic change cell culture workstation.
Background
The automatic cell culture workstation can realize the automatic culture of cells, and in the cell culture process, the prevention of cell contamination is the key for determining the success or failure of cultured cells. Therefore, cell manipulation for application in automated cell culture workstations needs to meet the following requirements: 1. operating in a sterile and clean environment; 2. in the cell batch switching process, in order to avoid cross contamination among cells in the workstation, the internal environment of the workstation is disinfected in a preventive manner; 3. when pollution occurs, the internal space of the workstation and the surface of the equipment can be disinfected and sterilized integrally.
In order to meet the requirements of the cell culture environment and improve the capability of the automated cell culture workstation to deal with the cell pollution problem, the inside of the workstation is generally subjected to pollution elimination by adopting a manual operation mode, and the mode has the following defects: 1. an open space is formed in the process of manually placing and taking out the sterilizer, so that potential pollution inside the workstation can be leaked; 2. the disinfection and sterilization agent naturally diffuses in the workstation, so that the disinfection and sterilization agent in the workstation is unevenly distributed, the diffusion time is long, and the disinfection efficiency is low; 3. the sterilization dosage in the workstation cannot be judged, and excessive disinfectant not only causes resource waste, but also accelerates the corrosion of equipment materials in the workstation; and excessive residual disinfectants can also have an impact on cell health.
In order to solve the above technical problems, the invention patent with application publication No. CN 104498359A discloses an integrated cell culture machine, which can realize sterilization in the machine body, but in the scheme, in order to realize air circulation in the sterile chamber, air in the sterile chamber is led out and then led into the sterile chamber under the action of a fan, so as to form air circulation in the inner periphery and the outer periphery of the sterile chamber. The structure can cause potential pollution in the induced air pipeline, and cross contamination is easy to cause when cell batches are switched.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the technical problem that the existing automatic cell culture workstation has low sterilization efficiency and is easy to cause cross contamination.
The utility model provides an automatic change cell culture workstation, automatic change cell culture workstation includes the box, be equipped with the first cavity and the second cavity that are linked together in the box, still include degassing unit, degassing unit is including the container, the orientation that hold disinfectant the inside injection apparatus who sprays disinfectant of first cavity and/or second cavity, the container with the injection apparatus intercommunication, still be equipped with first fan in the first cavity, still be equipped with the second fan in the second cavity, the air-out direction opposite direction setting of first fan and second fan.
Under the condition that adopts above-mentioned technical scheme, the utility model discloses a be equipped with degassing unit, can realize the disinfection to the internal environment of cavity, reduce cell pollution and take place probability, improve the cell culture success rate. Through being equipped with first fan and second fan, can promote the circulation of the interior air of cavity, guarantee the evenly distributed of disinfectant in the cavity. Simultaneously, the air-out direction opposite direction setting of first fan and second fan makes the distance between two fans apart from the farthest in limited cavity space, and the air-out of two fans forms the convection current in the cavity to make the air in the cavity form the convection current circulation, can realize the fastest air mixing of the disinfectant that erupts in the cavity, thereby accelerate the evenly distributed of disinfectant in the cavity, shorten long when hydrogen peroxide sprays, improve disinfection and isolation efficiency. In addition, the first fan and the second fan are both arranged inside the chamber, only the air inside the chamber is disturbed, and the pollution in the chamber cannot be brought out, so that the phenomenon of cross contamination cannot occur when cell batches are switched.
In a specific embodiment of the above automated cell culture workstation, the first blower is disposed at a higher height on a side wall of the first chamber than the second blower is disposed at a side wall of the second chamber.
In a specific embodiment of the above automated cell culture workstation, the first fan is disposed at a top end of a side wall of the first chamber, and the second fan is disposed at a bottom end of a side wall of the second chamber.
In one embodiment of the above automated cell culture workstation, the number of the spraying devices is at least two, wherein one of the spraying devices and the other of the spraying devices are disposed in the first chamber or the second chamber on opposite sides.
Under the condition of adopting the technical scheme, the spraying devices in the cavities form a spraying state, the spraying time is shortened, and the disinfection and sterilization efficiency is improved.
In a particular embodiment of the above automated cell culture workstation, further comprising a vaporization generator disposed between the container and the sparging device.
Under the condition of adopting the technical scheme, the vaporization generator vaporizes the disinfectant in the container, so that the disinfectant forms vaporized particles.
In a particular embodiment of the above automated cell culture workstation, further comprising a pump device, the pump device being disposed between the container and the sparging device.
In a specific embodiment of the above automated cell culture workstation, the automated cell culture workstation further comprises a control module, and the control module is in communication connection with the vaporization generator, the first fan, the second fan and the pump device, respectively.
Under the condition of adopting above-mentioned technical scheme, can realize automatic disinfection, need not artifical manually operation, liberation both hands not only improves disinfection and isolation work efficiency to can reduce the contact of operating personnel and disinfectant, the protection operating personnel is healthy and safe.
In a specific embodiment of the above automated cell culture workstation, the automated cell culture workstation further comprises a disinfectant concentration detection sensor, the disinfectant concentration detection sensor is in communication connection with the control module, and the disinfectant concentration detection sensor is disposed in the first chamber and/or the second chamber.
Under the condition of adopting above-mentioned technical scheme, disinfectant concentration detection sensor can real-time supervision intracavity disinfectant's concentration to guarantee that the disinfection dose in the cavity is in reasonable within range, thereby can guarantee disinfection effect and cell safety.
In the above embodiment of the automated cell culture workstation, the automated cell culture workstation further comprises an angle adjusting assembly, and the spraying device is connected with the angle adjusting assembly.
Under the condition of adopting the technical scheme, the spraying device can realize the adjustment of the spraying angle, increase the spraying range of the disinfectant and improve the spraying efficiency.
In the above specific embodiment of the automated cell culture workstation, the automated cell culture workstation further comprises an air exhaust device, and the air exhaust device is disposed on the first chamber and/or the second chamber.
Under the condition of adopting above-mentioned technical scheme, can realize discharging the residual disinfectant in the cavity, avoid causing the influence because of remaining disinfectant to the cell health to cooperate with disinfectant concentration detection sensor and use, can the scientific control disinfectant residual amount, guarantee cell safety.
Drawings
Preferred embodiments of the present invention are described below with reference to the accompanying drawings, in which:
FIG. 1 is a top view of the automated cell culture workstation of the present invention;
FIG. 2 is a control schematic diagram of the automated cell culture workstation of the present invention;
FIG. 3 is a schematic diagram of the sterilization apparatus in a second embodiment of the automated cell culture workstation of the present invention;
list of drawings:
1. a storage chamber; 2. an operating room; 3. a culture chamber; 4. an electric cabinet; 5. a first fan; 6. a second fan; 7. a disinfectant concentration detection sensor; 8. an injection device; 9. a vaporization generator; 10. a pump device; 11. a container; 12. an air exhaust device; 13. and a control module.
Detailed Description
Preferred embodiments of the present invention will be described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only for explaining the technical principle of the present invention, and are not intended to limit the scope of the present invention. And can be adjusted as needed by those skilled in the art to suit particular applications. For example, although the description has been made in connection with a hydrogen peroxide disinfectant, it will be apparent that the present invention may be used in various other forms of disinfectant, so long as the disinfectant is suitable for use in the field of cell culture.
It should be noted that in the description of the present invention, the terms "top end", "bottom end", "inner", "outer", etc. indicating directions or positional relationships are based on the directions or positional relationships shown in the drawings, which are only for convenience of description, and do not indicate or imply that the related devices or elements must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Moreover, the ordinal numbers "first", "second", etc., are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Furthermore, it should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "mounted" and "connected" are to be interpreted broadly, and may be, for example, a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Example 1
At first refer to figure 1, the utility model provides an automatic change cell culture workstation, automatic cell culture workstation include the box, are equipped with the first cavity and the second cavity that are linked together in the box. In the present embodiment, the first chamber is a storage chamber 1, and the second chamber is an operation chamber 2. The first chamber can be the operation chamber 2 and the second chamber can be the storage chamber 1, as required by those skilled in the art.
In addition, a culture room 3 and an electric cabinet 4 are arranged in the box body.
With continued reference to FIG. 1, the automated cell culture workstation further comprises a sterilizing device comprising a container 11 (not shown in FIG. 1) for holding a sterilizing agent and a spraying device 8, the container 11 being in communication with the spraying device 8. The disinfectant contained in the container 11 is hydrogen peroxide disinfectant. And the spraying devices 8 are arranged at the tops of the first chamber and the second chamber, the number of the spraying devices 8 in each chamber is selectively set according to the space, and the spraying devices 8 arranged on each chamber respectively spray disinfectant towards the interior of each chamber so as to sterilize each chamber. In addition, since the first chamber is communicated with the second chamber, under the condition that the space allows, a person skilled in the art can also selectively arrange the spraying device 8 at the top of the first chamber or the second chamber to sterilize each chamber.
In the present embodiment, the container 11 and the spraying device 8 may be in direct communication or in communication via a pipeline, as required by those skilled in the art. Between the container 11 and the spraying devices 8, there may be a plurality of spraying devices 8 corresponding to one container 11, the container 11 delivering the sterilizing agent to each spraying device 8, or there may be a plurality of spraying devices 8 corresponding to one container 11, the container 11 delivering the sterilizing agent only to the spraying device 8 corresponding thereto.
To achieve the delivery of the disinfectant in the container 11, a pump device 10 is provided between the container 11 and the spraying device 8. Specifically, the pump device 10 is a liquid supply pump, and the injection device 8 is an atomizing nozzle. The hydrogen peroxide disinfectant in the container 11 is conveyed to the atomizing nozzle under the power action of the liquid supply pump, and the hydrogen peroxide disinfectant forms mist-shaped liquid drops through the atomizing nozzle to be sprayed into the cavity. In addition, the spraying device 8 is not limited to the atomizing nozzle mentioned in the embodiment, and a person skilled in the art may select other nozzles according to actual needs to be able to spray the hydrogen peroxide disinfectant into the chamber.
In order to enable the injection device 8 to realize the adjustment of the injection angle, in the present embodiment, an angle adjusting assembly (not shown in the figure) is further included, and the injection device 8 is connected with the angle adjusting assembly. Specifically, the angle adjustment assembly is a mechanical structure that is conventionally adopted by those skilled in the art, and is not described herein again.
Continuing to refer to fig. 1, a first fan 5 is further disposed in the first chamber, a second fan 6 is further disposed in the second chamber, and the air outlet directions of the first fan 5 and the second fan 6 are opposite. Wherein, the arrow direction shown in fig. 1 is the air outlet direction. In the present embodiment, the first fan 5 and the second fan 6 are impeller fans. Specifically, the first fan 5 is disposed on the left side wall of the first chamber, and the second fan 6 is disposed on the right side wall of the second chamber. Alternatively, the first fan 5 is disposed on the front side wall of the first chamber, and the second fan 6 is disposed on the rear side wall of the second chamber. Alternatively, the first fan 5 is disposed on a rear side wall of the first chamber, and the second fan 6 is disposed on a front side wall of the second chamber. And, the installation height of the first fan 5 and the second fan 6 in the corresponding chamber includes but is not limited to the following cases:
(1) The first fan 5 and the second fan 6 are arranged at the same height, namely the height of the first fan 5 from the ground is consistent with the height of the second fan 6 from the ground.
(2) The first fan 5 and the second fan 6 have a height difference, that is, the height of the first fan 5 from the ground can be higher than the height of the second fan 6 from the ground, and the height of the first fan 5 from the ground can also be lower than the height of the second fan 6 from the ground. Preferably, the first fan 5 is arranged at the intersection of three side walls of the first chamber, the second fan 6 is arranged at the intersection of three side walls of the second chamber, so that the first fan 5 and the second fan 6 are furthest arranged in a diagonal manner, and the circulating air coverage in the first chamber and the second chamber can be ensured to be maximum during operation, so that the hydrogen peroxide disinfectant coverage in the chambers is most uniform. For example, the first fan 5 is disposed at the intersection of the left side wall, the top and the rear side wall of the first chamber, and the second fan 6 is disposed at the intersection of the right side wall, the bottom and the front side wall of the second chamber.
In particular, by providing the first fan 5 and the second fan 6, the circulation of the air inside the chamber can be promoted, ensuring a uniform distribution of the disinfectant inside the chamber. Simultaneously, the air-out direction opposite direction setting of first fan 5 and second fan 6 makes the distance between two fans apart from the farthest in limited cavity space, and the air-out of two fans forms the convection current in the cavity to make the air in the cavity form the convection current circulation, can realize the fastest mixed wind of the disinfectant that erupts in the cavity, thereby accelerate the evenly distributed of disinfectant in the cavity, shorten hydrogen peroxide and spray when long, improve disinfection and isolation efficiency. In addition, the first fan 5 and the second fan 6 are both arranged inside the chamber, only disturbance is carried out on air inside the chamber, pollution in the chamber cannot be brought out, and therefore cross pollution cannot occur when cell batches are switched.
The disinfectant concentration monitoring device is characterized by further comprising a disinfectant concentration detection sensor 7, wherein the disinfectant concentration detection sensor 7 is arranged in the first cavity and/or the second cavity and is used for monitoring the concentration of disinfectant in the cavity in real time. Specifically, in the present embodiment, the disinfectant concentration detection sensor 7 is a hydrogen peroxide concentration detection sensor. Further, the number of the disinfectant concentration detection sensors 7 to be provided and the positions thereof may be adaptively selected as necessary by those skilled in the art. Preferably, the disinfectant concentration detection sensors 7 are respectively and correspondingly arranged in the farthest range of the spraying device 8 in the corresponding chamber.
The device also comprises an air exhaust device 12, and the air exhaust device 12 is arranged on the first chamber and/or the second chamber. Specifically, the exhaust device 12 includes an exhaust duct and an exhaust fan disposed in the exhaust duct, and the exhaust duct is communicated with an exhaust port on the chamber to realize exhaust inside the chamber. In this embodiment, the exhaust port is provided at the lower portion of the chamber.
Referring to fig. 2, the electronic device further includes a control module 13, the control module 13 is disposed in the electric cabinet 4, and the control module 13 may adopt an MCU control chip. The control module 13 is in communication connection with the first fan 5, the second fan 6, the exhaust fan, the pump device 10 and the disinfectant concentration detection sensor 7, respectively. Wherein, control module 13 is in order to control opening of first fan 5, second fan 6, air exhaust fan and pump unit 10 respectively and to open, and control module 13 is in order to receive the disinfectant concentration that disinfectant concentration detection sensor 7 monitored in real time to can be according to the disinfectant concentration that monitors with opening of control air exhaust fan and pump unit 10 and stop.
Specifically, when disinfection is required, the control module 13 controls the pump device 10 to operate, so that the disinfectant in the container 11 is delivered to the spraying device 8, and the disinfectant in the chamber is sprayed. The control module 13 controls the first fan 5 and the second fan 6 to work, so that the circulating convection of the air in the chamber is realized, and the sprayed disinfectant is uniformly distributed in the chamber. Disinfectant concentration in each chamber of disinfectant concentration detection sensor 7 real-time supervision, give control module 13 with disinfectant concentration real-time transfer, control module 13 and compare the disinfectant concentration that receives with predetermined disinfectant concentration, if the disinfectant concentration that receives reaches predetermined first disinfectant concentration, control module 13 control pump unit 10 stop work, make injection apparatus 8 stop spraying, and delay control air exhaust fan work, when injection apparatus 8 stops spraying promptly, start the disinfection time countdown, and open air exhaust apparatus 12 work automatically when the disinfection finishes. When the exhaust device 12 exhausts air, the disinfectant concentration detection sensor 7 can continuously monitor the concentration of the disinfectant in each chamber in real time, and if the concentration of the received disinfectant is lower than the concentration of the preset second disinfectant, the control module 13 controls the exhaust fan to stop working and prompts workers to finish disinfection. Wherein, for the preset first disinfectant concentration value, the second disinfectant concentration value and the disinfection time, the technicians in the field can set the concentration values according to the actual needs.
Example 2
This embodiment is different from embodiment 1 in that, referring to fig. 3, a vaporization generator 9 is further included, and the vaporization generator 9 is disposed between the container 11 and the injection device 8. In this embodiment, the vaporization generator 9 is a hydrogen peroxide vaporization generator 9, and the spraying device 8 may be a nozzle. The vaporization generator 9 is in communication connection with the control module 13, and the control module 13 controls the vaporization generator 9 to work, so that the disinfectant in the container 11 forms vaporized particles and is sprayed into the cavity through the nozzle.
Example 3
This embodiment differs from embodiment 1 in that at least two injection devices 8 are provided, with one injection device 8 and the other injection device 8 being disposed in the first chamber or the second chamber on opposite sides.
In particular, the spraying means 8 are arranged at the top of the first chamber and, correspondingly, the spraying means 8 are arranged at opposite positions at the bottom of the first chamber, so that the two spraying means 8 form a spraying-on state, which ensures the efficiency of spraying the disinfectant inside the chamber. Similarly, the second chamber is provided with a spray device 8 at the top and correspondingly a spray device 8 at the bottom opposite the second chamber, so that the two spray devices 8 form a double spray. One skilled in the art can also selectively position the first chamber or the second chamber, or both the first chamber and the second chamber, as desired.
Above-mentioned embodiment and various expansion embodiments make the utility model discloses an automatic cell culture workstation is carrying out the during operation of automatic disinfection and isolation, can realize the air convection circulation of the inside airtight space of cavity, guarantees the evenly distributed of disinfection agent in the cavity, improves disinfection and isolation efficiency, and cross contamination and pollution can not appear leaking. Meanwhile, manual operation is replaced, the working efficiency of disinfection and sterilization is improved, contact between operators and disinfectants can be reduced, the health and safety of the operators are protected, the clean environment of cell culture in a workstation can be effectively maintained and guaranteed, and the success rate of cell culture is improved.
So far, the technical solution of the present invention has been described with reference to the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, a person skilled in the art can make equivalent changes or substitutions to the related technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.
Claims (10)
1. An automatic cell culture workstation comprises a box body, a first chamber and a second chamber which are communicated are arranged in the box body,
the disinfectant spraying device is characterized by further comprising a disinfecting device, wherein the disinfecting device comprises a container for containing disinfectant and a spraying device for spraying the disinfectant towards the inside of the first cavity and/or the inside of the second cavity, the container is communicated with the spraying device, a first fan is further arranged in the first cavity, a second fan is further arranged in the second cavity, and the air outlet directions of the first fan and the second fan are opposite.
2. The automated cell culture workstation of claim 1, wherein the first blower is disposed at a higher elevation on a sidewall of the first chamber than the second blower is disposed at a sidewall of the second chamber.
3. The automated cell culture workstation of claim 2, wherein the first fan is disposed at a top end of a side wall of the first chamber and the second fan is disposed at a bottom end of a side wall of the second chamber.
4. The automated cell culture workstation of claim 1, wherein at least two of the jetting devices are provided, wherein one of the jetting devices and the other of the jetting devices are disposed in the first chamber or the second chamber on opposite sides.
5. The automated cell culture workstation of claim 1, further comprising a vaporization generator disposed between the container and the sparging device.
6. The automated cell culture workstation of claim 5, further comprising a pump device disposed between the container and the sparging device.
7. The automated cell culture workstation of claim 6, further comprising a control module in communication with the vaporization generator, the first fan, the second fan, and the pump device, respectively.
8. The automated cell culture workstation of claim 7, further comprising a disinfectant concentration detection sensor in communicative connection with the control module, the disinfectant concentration detection sensor disposed within the first chamber and/or the second chamber.
9. The automated cell culture workstation of claim 1, further comprising an angle adjustment assembly, the sparging device being connected to the angle adjustment assembly.
10. The automated cell culture workstation of claim 1, further comprising an air exhaust device disposed on the first chamber and/or the second chamber.
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CN202222034619.7U CN218860754U (en) | 2022-08-03 | 2022-08-03 | Automatic cell culture workstation |
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CN202222034619.7U CN218860754U (en) | 2022-08-03 | 2022-08-03 | Automatic cell culture workstation |
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