CN218860741U - Measurement and control system for preparing single-cell micro-droplets and single-cell micro-droplet preparation equipment - Google Patents
Measurement and control system for preparing single-cell micro-droplets and single-cell micro-droplet preparation equipment Download PDFInfo
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- CN218860741U CN218860741U CN202221977271.9U CN202221977271U CN218860741U CN 218860741 U CN218860741 U CN 218860741U CN 202221977271 U CN202221977271 U CN 202221977271U CN 218860741 U CN218860741 U CN 218860741U
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Abstract
The utility model provides a measurement and control system and unicellular micro-droplet preparation equipment for preparation of unicellular micro-droplet relates to unicellular sequencing technical field, the measurement and control system includes: host system, two at least electronic pressure control module, two at least atmospheric pressure detection module and two at least air current detection module, wherein: the electronic pressure control module, the air pressure detection module and the air flow detection module are in communication connection with the main control module and are all arranged in the air flow channel, wherein the electronic pressure control module is used for controlling the air flow required by the air flow channel in real time, the air pressure detection module is used for measuring the air pressure in the air flow channel, and the air flow detection module is used for measuring the air flow in the air flow channel; the main control module is used for controlling the air pressure and the air flow in the air flow channel of the single-cell micro-droplet chip in real time and judging whether the air flow channel leaks air or not. The utility model discloses can ensure the atmospheric pressure stability in the little liquid drop preparation process of unicellular to improve and prepare the qualification rate.
Description
Technical Field
The utility model relates to a unicellular sequencing technology field especially relates to a preparation of unicellular microdroplet is with survey control system and unicellular microdroplet preparation equipment.
Background
In recent years, single cell microfluidic technology has become one of the conventional research techniques in single cell sequencing. In single cell sequencing technology, the preparation of single cell micro-droplets is a key step in single cell sequencing. In the process of sequencing single cells by using the microfluidic technology, single cells need to be captured, that is, single cells are separated from a micron-sized single-cell micro-droplet chip channel and fixed at a specific position so as to perform subsequent operation and analysis and detection.
At present, for most single-cell micro-droplet preparation devices, the number, size and uniformity of the generated single-cell micro-droplets are important parameters for measuring the performance index of the single-cell micro-droplet preparation device. However, the conventional microfluidic devices mostly adopt the following two methods for performing the microfluidic control:
in the micro-injection pump mode, the air pressure stability is poor, so that the preparation qualification rate of the single-cell micro-droplets is relatively low, and the scientific research work in related fields is limited;
however, the stability of the pressure output in this method is poor, and the requirement of single cell sequencing application cannot be met well under the condition of high requirement of the pressure stability.
SUMMERY OF THE UTILITY MODEL
The utility model provides a measurement and control system and unicellular micro-droplet preparation equipment are used in preparation of unicellular micro-droplet for solve among the prior art the relatively poor and lower defect of preparation qualification rate of atmospheric pressure stability, ensure the atmospheric pressure stability in the preparation of unicellular micro-droplet, further improve the preparation qualification rate of unicellular micro-droplet.
The utility model provides a measurement and control system is used in preparation of unicellular micro-droplet, include: host system, two at least electronic pressure control module, two at least atmospheric pressure detection module and two at least air current detection module, wherein:
the electronic pressure control module, the air pressure detection module and the air flow detection module are all in communication connection with the main control module and are all arranged in the air flow channel, wherein the electronic pressure control module is used for controlling the air flow required by the air flow channel in real time, the air pressure detection module is used for measuring the air pressure in the air flow channel, and the air flow detection module is used for measuring the air flow in the air flow channel;
the main control module is used for controlling the air pressure and the air flow in an air flow channel of the single-cell micro-droplet chip in real time and judging whether the air flow channel leaks air or not.
According to the utility model provides a measurement and control system is used in preparation of unicellular micro-droplet, atmospheric pressure detection module includes: the air pressure sensor is in communication connection with the first signal conditioning unit, and the first signal conditioning unit is in communication connection with the main control module and is used for receiving and forwarding an air pressure detection instruction issued by the main control module.
According to the utility model provides a measurement and control system is used in preparation of unicellular micro-droplet, the air current detection module includes: the gas flow sensor is in communication connection with the second signal conditioning unit, and the second signal conditioning unit is in communication connection with the main control module and is used for receiving and forwarding a gas flow detection instruction issued by the main control module.
According to the utility model provides a measurement and control system is used in preparation of unicellular micro-droplet still includes:
and the air pump driving circuit module is respectively in communication connection with the air pump and the main control module and is used for driving the air pump to generate the air pressure required by the preparation of the unicellular micro-droplets according to the instruction of the main control module.
According to the utility model provides a measurement and control system is used in preparation of unicellular little liquid drop still includes:
and the touch display screen module is in communication connection with the main control module and is used for inputting system control parameters and displaying acquired data.
According to the utility model provides a measurement and control system is used in preparation of unicellular micro-droplet still includes:
and the sound-light alarm module is in communication connection with the main control module and is used for giving an alarm under the condition that the air leakage of the air flow channel is detected.
According to the utility model provides a measurement and control system is used in preparation of unicellular micro-droplet still includes:
and the electromagnetic valve driving circuit module is in communication connection with the electromagnetic valve and the main control module and is used for adjusting the closing state of the electromagnetic valve in the airflow channel and changing the airflow direction in the airflow channel.
According to the utility model provides a measurement and control system is used in preparation of unicellular micro-droplet still includes:
and the data storage module is in communication connection with the main control module, and a user stores the data collected in the airflow channel.
According to the utility model provides a measurement and control system is used in preparation of unicellular micro-droplet still includes:
the main control module is in communication connection with the electronic pressure control module, the air pressure detection module, the airflow detection module, the air pump driving circuit module, the touch display screen module, the sound-light alarm module, the electromagnetic valve driving circuit module and the data storage module through the communication bus respectively.
The utility model also provides a unicellular micro-droplet preparation equipment, include as above-mentioned arbitrary above unicellular micro-droplet preparation with measurement and control system.
The utility model provides a measurement and control system and unicellular micro-droplet preparation equipment are used in preparation of unicellular micro-droplet, through host system control electronic pressure control module, required atmospheric pressure in each airflow channel when accurate control supplies with unicellular micro-droplet preparation, and through atmospheric pressure and the air flow in atmospheric pressure detection module and the real-time detection of airflow detection module correspond airflow channel, and judge whether air leakage of airflow channel is based on above-mentioned atmospheric pressure and air flow, under the condition of air leakage, prepare with stable atmospheric pressure, stop the preparation under the condition of air leakage, and then avoid preparing out the unicellular micro-droplet that does not satisfy the requirement, further improve the qualification rate of unicellular micro-droplet preparation.
Drawings
In order to more clearly illustrate the technical solutions of the present invention or the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural view of a measurement and control system for preparing single-cell micro-droplets provided by the present invention;
fig. 2 is a schematic structural diagram of the air pressure detecting module provided by the present invention;
fig. 3 is a schematic structural diagram of an airflow detection module provided by the present invention;
FIG. 4 is a schematic flow chart of the measurement and control system for preparing single-cell micro-droplets provided by the present invention.
Detailed Description
To make the objects, technical solutions and advantages of the present invention clearer, the drawings of the present invention are combined to clearly and completely describe the technical solutions of the present invention, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
At present, a micro-fluidic control device is mostly adopted for preparing single-cell micro-droplets, and in the micro-fluidic control device, a micro-injection pump mode and a mode of adjusting air pressure output in real time are generally adopted for micro-flow control, however, when the micro-injection pump mode is adopted, the qualified rate of preparing the single-cell micro-droplets is relatively low, and the rate of preparing the qualified single-cell micro-droplets is relatively slow, so that the scientific research work of related fields is limited; when the mode of adjusting the air pressure output in real time is adopted, the stability of the air pressure output is poor, and the requirements of the single cell sequencing application at present cannot be well met under the condition of high requirement of the air pressure stability. In addition, the micro-fluidic device has a complex composition structure, has the obvious defects of large volume, heavy weight, difficulty in carrying, unfriendly operation interface and the like, and has the serious defects of monopoly foreign countries and the like.
To the above problem, the utility model provides a little liquid drop of unicellular preparation is with observing and controling system, figure 1 is the utility model provides a little liquid drop of unicellular preparation is with observing and controlling system's schematic structure, as shown in figure 1, this observing and controlling system includes: the main control module 100, at least two electronic pressure control modules 101, at least two air pressure detection modules 102, and at least two air flow detection modules 103, wherein:
the electronic pressure control module 101, the air pressure detection module 102, and the air flow detection module 103 are all in communication connection with the main control module 100, and are all disposed in the air flow channel, wherein the electronic pressure control module 101 is configured to control a flow rate of air required to be supplied to the air flow channel in real time, the air pressure detection module 102 is configured to measure an air pressure in the air flow channel, and the air flow detection module 103 is configured to measure a flow rate of air in the air flow channel;
the main control module 100 is configured to control the air pressure and the air flow in the air flow channel of the single-cell micro droplet chip in real time and determine whether the air flow channel leaks air.
Specifically, because the rate of the qualified unicellular little liquid drop of preparation of current scheme is lower and atmospheric control stability is lower, lead to preparing the qualification rate lower, therefore, the utility model discloses in, according to the atmospheric pressure parameter of user input, atmospheric pressure in adjusting each airflow channel through a plurality of electron pressure control module 101 of host system 100 control, make and stably supply atmospheric pressure when predetermineeing, and then each airflow channel through air pressure drive unicellular little liquid drop chip, utilize the interact preparation unicellular little liquid drop between little liquid drop flow shearing force and the surface tension, because atmospheric pressure in each airflow channel is stable, make the size of the unicellular little liquid drop of preparation unified, further improve homogeneity and the qualification rate that unicellular little liquid drop prepared, each electron pressure control module 101 is independent each other, each other does not influence, improve the preparation efficiency of unicellular little liquid drop. Meanwhile, in the preparation process of the single-cell micro-droplets, an air pressure detection module 102 and an air flow detection module are also arranged in each air flow channel, and whether air leaks in the air flow channel is judged by detecting the air pressure and the air flow in each air flow channel in real time, so that the single-cell micro-droplets with the sizes not meeting the requirements are prevented from being prepared under the condition of air leakage, and the qualified rate is reduced.
Optionally, the preset time period may be a preparation time period, that is, within the preset time period, stable air pressure may be provided by the electronic pressure control module 101 to prepare the single-cell micro-droplet, and after the preparation time period reaches the preset time period, the air pressure value in the corresponding air flow channel is adjusted to be a 0 pressure value, so as to finish the preparation.
Optionally, the number of the air flow channels, the number of the electronic pressure control modules 101, the number of the air pressure detection modules 102, and the number of the air flow detection modules 103 are all equal, or the number of the air flow channels is greater than the number of the electronic pressure control modules 101, the number of the air pressure detection modules 102, and the number of the air flow detection modules 103 are all equal, that is, there may be an empty air flow channel in the single-cell micro-droplet chip.
Optionally, fig. 2 is a schematic structural diagram of the air pressure detecting module 102 provided by the present invention, as shown in fig. 2, the air pressure detecting module 102 includes: the pressure sensor 1011 is in communication connection with the first signal conditioning unit 1012, and the first signal conditioning unit 1012 is in communication connection with the main control module 100 and is used for receiving and forwarding a pressure detection instruction issued by the main control module 100.
Specifically, when the air pressure detection module 102 performs air pressure detection, the first signal conditioning unit 1012 receives a control instruction of the main control module 100 and transmits the control instruction to the air pressure sensor 1011, and the air pressure sensor 1011 detects air pressure data in a corresponding air flow channel in real time and uploads the air pressure data to the main control module 100 through the first signal conditioning unit 1012, so as to perform subsequent judgment and data storage.
Optionally, fig. 3 is a schematic structural diagram of the airflow detecting module 103 provided by the present invention, as shown in fig. 3, the airflow detecting module 103 includes: the gas flow sensor 1021 and the second signal conditioning unit 1022 are in communication connection, the gas flow sensor 1021 is in communication connection with the second signal conditioning unit 1022, and the second signal conditioning unit 1022 is in communication connection with the main control module 100, and is configured to receive and forward a gas flow detection instruction issued by the main control module 100.
Specifically, when the airflow detection module 103 detects airflow, the second signal conditioning unit 1022 receives a control instruction of the main control module 100 and transmits the control instruction to the airflow sensor 1021, and the airflow sensor 1021 detects airflow data in the airflow channel in real time and then uploads the data to the main control module 100 through the second signal conditioning unit 1022, so as to perform subsequent determination and data storage.
Optionally, the measurement and control system further includes:
and the air pump driving circuit module 104 is respectively in communication connection with the air pump and the main control module 100, and is used for driving the air pump to generate the air pressure required by the preparation of the unicellular micro-droplets according to the instruction of the main control module 100.
Specifically, the main control module 100 drives the motor of the air pump to rotate through the air pump driving circuit module 104, so as to generate air pressure, so that the measurement and control system can prepare single-cell micro-droplets.
Optionally, the measurement and control system further includes:
and the touch display screen module 105 is in communication connection with the main control module 100 and is used for inputting system control parameters and displaying acquired data.
Specifically, before the preparation of the single-cell micro-droplet, a user may edit and input the air pressure parameters in each air flow channel and the preset time length of the corresponding air flow channel when the single-cell micro-droplet is prepared through the touch display screen module 105, and send the preset parameters to the main control module 100, so that the main control module 100 can provide stable air pressure according to the set parameters to prepare the single-cell micro-droplet. In addition, in the preparation process, after the data collected by the air pressure detection module 102 and the airflow detection module 103 are uploaded to the main control module 100, the main control module 100 sends the data to the touch display screen module 105 for data display, so that the data display is clear and intuitive.
Optionally, the measurement and control system further includes:
and the sound and light alarm module 106 is in communication connection with the main control module 100 and is used for giving an alarm when the air leakage of the air flow channel is detected.
Specifically, after the detection data in each airflow channel is acquired in the preparation process, the real-time detection data is compared with the preset air pressure value in the corresponding airflow channel, if the real-time detection data is smaller than the preset parameter value, air leakage occurs, and the main control module 100 reminds the user of preparation failure through the sound-light alarm module 106, so as to perform failure removal.
Optionally, the sound and light alarm module 106 may be a buzzer or a flash lamp, or may be an intelligent device with a broadcasting function such as an intelligent sound, and the sound and light alarm module reminds a user of troubleshooting by reporting that a fault occurs in the preparation process.
Optionally, the measurement and control system further includes:
and the electromagnetic valve driving circuit module 107 is in communication connection with the electromagnetic valve and the main control module 100, and is used for adjusting the closing state of the electromagnetic valve in the airflow channel and changing the airflow direction in the airflow channel.
Specifically, an electromagnetic valve is arranged in each airflow channel, and the electromagnetic valve is closed by controlling the electromagnetic valve driving circuit module 107 through the main control module 100, so that the inside of the airflow channel is kept in a sealed state, and further, the required air pressure is provided for the inside of the airflow channel. In addition, after the preparation is finished, the electromagnetic valve in the airflow channel can be opened to release the pressure.
Optionally, the measurement and control system further includes:
and the data storage module 108 is in communication connection with the main control module 100, and a user stores the data collected in the airflow channel.
Specifically, after the detection data is acquired in the preparation process, the detection data is uploaded to the main control module 100 to judge whether air leakage occurs, and meanwhile, the main control module 100 sends the detection data to the data storage module 108 to store the detection data so as to perform troubleshooting after air leakage occurs.
Optionally, the measurement and control system further includes:
the main control module 100 is in communication connection with the electronic pressure control module 101, the air pressure detection module 102, the airflow detection module 103, the air pump driving circuit module 104, the touch display screen module 105, the sound and light alarm module 106, the electromagnetic valve driving circuit module 107, and the data storage module 108 through the communication bus.
Fig. 4 is the utility model provides a little liquid drop of unicellular preparation is with measurement and control system's flow schematic diagram, as shown in fig. 4, the utility model provides a little liquid drop of unicellular preparation is with measurement and control system's use flow as follows:
(1) An operator edits and inputs air pressure parameters corresponding to each air flow channel and preset duration corresponding to each air flow channel through the touch display screen module 105, and sends the preset parameters to the main control module 100 through the communication bus;
(2) After receiving the set parameters sent by the touch display screen module 105, the main control module 100 firstly controls the air pump driving circuit module 104 to drive the air pump motor to rotate so as to generate the air pressure required by each airflow channel to prepare the single-cell micro-droplets, and meanwhile, sends the set parameters to the electronic pressure control module 101 corresponding to the airflow channel and starts a timing function, wherein the set duration is preset duration;
(3) The electronic pressure control module 101 adjusts the air pressure in the corresponding air flow channel according to the set parameters, so that the air pressure in the corresponding channel is equal to the set parameters, and the required single-cell micro liquid drop is generated by utilizing the interaction between the shearing force of the liquid drop flow and the surface tension;
(4) Meanwhile, in the process of preparing the single-cell micro-droplets, the main control module 100 controls the air pressure detection module 102 to detect the air pressure in the corresponding air flow channel in real time, controls the air flow detection module 103 to detect the air flow in the corresponding air flow channel in real time, and uploads the obtained real-time detection data to the main control module 100;
(5) The main control module 100 compares the real-time detection data with preset parameters before preparation to judge whether air leakage occurs, if the air leakage occurs, the main control module 100 controls the sound-light alarm module 106 to remind a user of the occurrence of a fault in the preparation process through modes of alarm sound, voice broadcast, flash and the like, so that the user is called to check the air leakage fault in the future, the air leakage fault is checked and solved, and the fault elimination process is displayed in real time through the touch display screen module 105; if no air leakage occurs, the preparation of the single-cell micro-droplets is continuously carried out, and under the condition that the preparation time reaches the preset time, the preparation is finished, namely, the air pressure value in each air flow channel is adjusted to be 0 pressure value.
Optionally, the utility model provides a measurement and control system is used in preparation of unicellular micro-droplet not only can be used to unicellular sequencing technical field, still can be applied to other fields such as medical analysis, medicine preparation.
The utility model provides a measurement and control system is used in preparation of unicellular microdroplet, through the 100 control electron pressure control module 101 of host system, required atmospheric pressure in each airflow channel when accurate control supplies with the preparation of unicellular microdroplet, and atmospheric pressure and gas flow in the corresponding airflow channel of real-time detection through atmospheric pressure detection module 102 and gas flow detection module 103, and judge whether gas flow channel leaks gas based on above-mentioned atmospheric pressure and gas flow, under the condition of not leaking gas, prepare with stable atmospheric pressure, stop the preparation under the condition of leaking gas, and then avoid preparing out the unsatisfactory unicellular microdroplet of requirement, further improve the qualification rate of unicellular microdroplet preparation and the efficiency of preparing qualified unicellular microdroplet. Furthermore, the utility model provides a measurement and control system is used in preparation of unicellular micro-droplet provides friendly human-computer interaction interface, easy operation, and simple as a result for the system volume is less, portable.
Based on above-mentioned preparation of unicellular micro-droplet is with measurement and control system, the utility model also provides a preparation of unicellular micro-droplet equipment, including the aforesaid preparation of unicellular micro-droplet is with measurement and control system.
The utility model provides a unicellular micro-droplet preparation equipment, through the control electronic pressure control module 101 of host system 100 control, required atmospheric pressure in each airflow channel when accurate control supplies with unicellular micro-droplet preparation, and atmospheric pressure and gas flow in corresponding airflow channel through atmospheric pressure detection module 102 and air current detection module 103 real-time detection, and judge whether air channel leaks gas based on above-mentioned atmospheric pressure and gas flow, under the condition of not leaking gas, prepare with stable atmospheric pressure, stop the preparation under the condition of leaking gas, and then avoid preparing the unicellular micro-droplet that does not satisfy the requirement, further improve the qualification rate of preparation unicellular micro-droplet in each airflow channel and the efficiency of preparation qualified unicellular micro-droplet, and the real-time detection through airflow channel, further improve the qualification rate of unicellular micro-droplet preparation. In addition, through a friendly human-computer interaction interface, the requirement on the professional degree of operators of the unicellular micro-droplet preparation equipment is reduced, the operation can be quickly performed, and the preparation efficiency is further improved.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications or substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. A measurement and control system for preparing single-cell micro-droplets is characterized by comprising: host system, two at least electron pressure control module, two at least atmospheric pressure detection module and two at least air current detection module, wherein:
the electronic pressure control module, the air pressure detection module and the air flow detection module are all in communication connection with the main control module and are all arranged in an air flow channel, wherein the electronic pressure control module is used for controlling the air pressure required by the air flow channel in real time, the air pressure detection module is used for measuring the air pressure in the air flow channel, and the air flow detection module is used for measuring the air flow in the air flow channel;
the main control module is used for controlling the air pressure and the air flow in the air flow channel of the single-cell micro-droplet chip in real time and judging whether the air flow channel leaks air or not.
2. The measurement and control system for single-cell micro-droplet preparation according to claim 1, wherein the air pressure detection module comprises: the air pressure sensor is in communication connection with the first signal conditioning unit, and the first signal conditioning unit is in communication connection with the main control module and is used for receiving and transmitting an air pressure detection instruction issued by the main control module.
3. The measurement and control system for single-cell micro-droplet preparation according to claim 1, wherein the gas flow detection module comprises: the gas flow sensor is in communication connection with the second signal conditioning unit, and the second signal conditioning unit is in communication connection with the main control module and is used for receiving and forwarding a gas flow detection instruction issued by the main control module.
4. The measurement and control system for single-cell microdroplet preparation according to any one of claims 1 to 3, further comprising:
and the air pump driving circuit module is respectively in communication connection with the air pump and the main control module and is used for driving the air pump to generate the air pressure required by the preparation of the single-cell micro-droplets according to the instruction of the main control module.
5. The measurement and control system for single-cell micro-droplet preparation according to claim 4, further comprising:
and the touch display screen module is in communication connection with the main control module and is used for inputting system control parameters and displaying acquired data.
6. The measurement and control system for single-cell micro-droplet preparation according to claim 5, further comprising:
and the sound-light alarm module is in communication connection with the main control module and is used for giving an alarm under the condition that the air leakage of the air flow channel is detected.
7. The measurement and control system for single-cell micro-droplet preparation according to claim 6, further comprising:
and the electromagnetic valve driving circuit module is in communication connection with the electromagnetic valve and the main control module and is used for adjusting the closing state of the electromagnetic valve in the airflow channel and changing the airflow direction in the airflow channel.
8. The measurement and control system for preparing single-cell micro-droplets according to claim 7, further comprising:
and the data storage module is in communication connection with the main control module, and a user stores the data collected in the airflow channel.
9. The measurement and control system for preparing single-cell micro-droplets according to claim 8, further comprising:
the main control module is in communication connection with the electronic pressure control module, the air pressure detection module, the airflow detection module, the air pump driving circuit module, the touch display screen module, the sound-light alarm module, the electromagnetic valve driving circuit module and the data storage module through the communication bus respectively.
10. A single-cell microdroplet preparation apparatus comprising the measurement and control system for single-cell microdroplet preparation according to any one of claims 1 to 9.
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|---|---|---|---|
| CN202221977271.9U CN218860741U (en) | 2022-07-28 | 2022-07-28 | Measurement and control system for preparing single-cell micro-droplets and single-cell micro-droplet preparation equipment |
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| CN202221977271.9U CN218860741U (en) | 2022-07-28 | 2022-07-28 | Measurement and control system for preparing single-cell micro-droplets and single-cell micro-droplet preparation equipment |
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