CN218842153U

CN218842153U - Anaerobic culture dish

Info

Publication number: CN218842153U
Application number: CN202222921253.5U
Authority: CN
Inventors: 刘文华; 林勇敢; 王盛洲; 何学松; 王京苏
Original assignee: Ganzhou Shanjian Biotechnology Co ltd
Current assignee: Ganzhou Shanjian Biotechnology Co ltd
Priority date: 2022-11-03
Filing date: 2022-11-03
Publication date: 2023-04-11
Anticipated expiration: 2032-11-03

Abstract

The invention relates to the field of biological culture, in particular to an anaerobic culture dish which comprises a barrel-shaped main body, wherein a supporting plate is arranged in the main body, a piston is further fixed on the supporting plate, the piston is arranged around the supporting plate, and an exhaust pipe, a liquid injection pipe and an air inlet pipe are further fixed on the supporting plate respectively. The anaerobic environment of anaerobic bacterial colony can be realized to this device to through the complete discharge of oxygen in the culture dish of culture solution, need not to use oxygen uptake agent such as pyrogallic acid, can effectively reduce cost and can effectively avoid oxygen uptake agent to mention and react with oxygen, and pyrogallic acid has the hypertoxic, and this device need not to use pyrogallic acid further to guarantee the security of operation.

Description

Anaerobic culture dish

[ technical field ] A

The invention relates to the field of biological culture, in particular to an anaerobic culture dish.

[ background of the invention ]

The cultivation of anaerobic microorganisms usually requires special equipment such as an anaerobic incubator to create an anaerobic environment and isolate the anaerobic microorganisms from oxygen. It is now common to culture anaerobic microorganisms by removing oxygen from a petri dish by reacting pyrogallic acid with a sodium hydroxide solution, as described in patent application No.: CN202120681460.0 discloses an anaerobic microorganism culture dish, the culture dish body includes outer ware, well ware and interior ware, puts into the inside of interior ware with the material that needs to cultivate, tears the inside sealing paper of first cavity, establishes the lid inside the second cavity, consumes the inside oxygen of culture dish body when the inside pyrogallic acid of first cavity uses, makes inside formation anaerobic environment. However, the following problems are encountered when the method is carried out using such a culture dish: the oxygen-eliminating agent placed in the dish is not easy to control, and before the cover body is covered after the sealing paper in the first cavity is torn, the pyrogallic acid can be contacted with oxygen in advance to react.

The present invention is developed in view of the shortcomings of the prior art.

[ summary of the invention ]

The object of the present invention is to overcome the above mentioned drawbacks of the prior art and to provide an anaerobic culture dish.

The invention can be realized by the following technical scheme:

the invention discloses an anaerobic culture dish which comprises a barrel-shaped main body, wherein a supporting plate is arranged in the main body, a piston is further fixed on the supporting plate, the piston is arranged around the supporting plate, and an exhaust pipe, a liquid injection pipe and an air inlet pipe are further respectively fixed on the supporting plate. When the culture dish used, push the backup pad to the culture dish bottom, in injecting the main part of backup pad below with the culture solution through annotating the liquid pipe, when the culture solution was injected through annotating the liquid pipe gradually, the air of backup pad below passes through the blast pipe and discharges, when the culture solution reachd annotates liquid pipe top position, seal the blast pipe through the sealing plug, realize that the backup pad below is anaerobic environment, after the blast pipe seals, again toward the backup pad below inject the culture solution, the culture solution promotes backup pad and piston and rises, after the volume of culture solution reached bacterial colony culture requirement, once more through the intake pipe toward the backup pad below inject nitrogen gas, nitrogen gas promotes backup pad and piston and rises, the backup pad has certain distance with the culture solution, conveniently observe bacterial colony growth, after injecting nitrogen gas, at last inject the bacterial colony once more through annotating the liquid pipe into the culture solution and breed the cultivation, the anaerobic environment of anaerobic bacterial colony can be realized to this device, and through the culture solution with the culture dish in the oxygen gas completely discharge, need not to use oxygen uptake agent such as pyrogallic acid, can effectively reduce the cost and can effectively avoid oxygen uptake agent to mention and oxygen reaction, and pyrogallic acid has toxicity, this device need not to further guarantee the security of operation.

Preferably, the support plate is of a conical structure, and the exhaust pipe is arranged in the middle of the support plate.

Preferably, the liquid injection pipe is further fixed with a bacterium injection pipe. And discharging the bacterial colony into a culture dish through a bacterium injection pipe.

Compared with the prior art, the invention has the following advantages:

the anaerobic environment of anaerobism bacterial colony can be realized to this device to discharge oxygen in with the culture dish completely through the culture solution, need not to use oxygen uptake agent such as burnt gallic acid, can effectively reduce cost and can effectively avoid oxygen uptake agent to mention and react with oxygen, and burnt gallic acid has the hypertoxic, and this device need not to use burnt gallic acid to further guarantee the security of operation.

[ description of the drawings ]

The following detailed description of embodiments of the invention is provided in conjunction with the appended drawings, in which:

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a top view of the present invention;

FIG. 3 isbase:Sub>A cross-sectional view taken at A-A of FIG. 2;

in the figure: 1. a main body; 2. a piston; 3. a support plate; 4. an exhaust pipe; 5. an air inlet pipe; 6. a liquid injection pipe; 7. injecting a bacteria tube;

[ detailed description ] embodiments

The embodiments of the present invention will be described in detail below with reference to the accompanying drawings:

example 1:

as shown in fig. 1 to 3, the present embodiment discloses an anaerobic culture dish, which comprises a barrel-shaped main body 1, a support plate 3 is arranged in the main body 1, a piston 2 is further fixed on the support plate 3, the piston 2 is arranged around the support plate 3, and an exhaust pipe 4, a liquid injection pipe 6 and an air inlet pipe 5 are further fixed on the support plate 3 respectively. When the culture dish uses, push backup pad 3 to the culture dish bottom, inject the culture solution into main part 1 of backup pad 3 below through annotating liquid pipe 6, when the culture solution is injected through annotating liquid pipe 6 gradually, the air of backup pad 3 below passes through blast pipe 4 and discharges, when the culture solution arrives annotates liquid pipe 6 top end position, seal blast pipe 4 through the sealing plug, realize that backup pad 3 below is anaerobic environment, after the seal of blast pipe 4, again toward the injection culture solution of backup pad 3 below, the culture solution promotes backup pad 3 and piston 2 and rises, after the volume of culture solution reaches the bacterial colony culture requirement, once more through intake pipe 5 toward backup pad 3 below injection nitrogen gas, nitrogen gas promotes backup pad 3 and piston 2 and rises, backup pad 3 has certain distance with the culture solution, conveniently observe the bacterial colony and grow, after injecting nitrogen gas into, breed the culture solution with the bacterial colony at last, the anaerobic environment of anaerobic bacterial colony can realize the anaerobic bacterial colony, and discharge the oxygen in the culture dish completely through the culture solution, need not use oxygen uptake agent such as burnt gallic acid, can effectively reduce cost and can effectively avoid oxygen uptake agent to mention and react with oxygen, and the further the operation scorching safety of gallic acid is guaranteed, this device, it has the violent toxicity to further use.

Wherein, the supporting plate 3 is a cone-shaped structure, and the exhaust pipe 4 is arranged in the middle of the supporting plate 3. The supporting plate 3 is of a conical structure, so that when the culture solution is injected, the air below the supporting plate can be completely discharged through the exhaust pipe 4, and the anaerobic environment in the culture dish is ensured.

Example 2:

the embodiment discloses an anaerobic culture dish, and based on the structure, principle and basis of the embodiment 1, a bacterium injection pipe 7 is further fixed on the liquid injection pipe 6 of the embodiment. The colonies are injected into the culture dish through the injection tube 7 alone.

The above description is only a preferred embodiment of the present invention, and it should be noted that a person skilled in the art can make various changes, modifications, substitutions and alterations to the embodiments without departing from the technical principles of the present invention, and such changes, modifications, substitutions and alterations should also be regarded as the protection scope of the present invention.

Claims

1. An anaerobic culture dish, includes the main part of tubbiness, its characterized in that: the novel liquid filling device is characterized in that a supporting plate is arranged in the main body, a piston is further fixed to the supporting plate, the piston is arranged around the supporting plate, an exhaust pipe, a liquid filling pipe and an air inlet pipe are further fixed to the supporting plate respectively, the supporting plate is of a conical structure, the exhaust pipe is arranged in the middle of the supporting plate, and the liquid filling pipe is further fixed to the liquid filling pipe.