CN218811709U - Escherichia coli engineering bacteria collecting device - Google Patents
Escherichia coli engineering bacteria collecting device Download PDFInfo
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- CN218811709U CN218811709U CN202222928526.9U CN202222928526U CN218811709U CN 218811709 U CN218811709 U CN 218811709U CN 202222928526 U CN202222928526 U CN 202222928526U CN 218811709 U CN218811709 U CN 218811709U
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- waste liquid
- collecting box
- liquid collecting
- escherichia coli
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The utility model provides an engineering bacteria collecting device for escherichia coli, which comprises an upper waste liquid collecting box, a peristaltic pump, a fermentation liquid collecting tank, a peristaltic pump and a fermentation liquid outlet, wherein the top of the upper waste liquid collecting box is used for receiving fermentation liquid pumped out from the fermentation liquid accumulating tank; the filtering unit is arranged in the upper waste liquid collecting box, the bottom of the filtering unit is positioned above the fermentation liquor outlet, and a spring is arranged between the filtering unit and the inner wall of the upper waste liquid collecting box; the top of the lower waste liquid collecting box is open, the bottom of the lower waste liquid collecting box is closed, a cavity for receiving liquid flowing out from the bottom of the filtering unit is formed inside the lower waste liquid collecting box, and the side wall of the lower waste liquid collecting box, which is positioned inside the lower waste liquid collecting box, is also integrally extended towards the inner side horizontally to form a supporting surface structure for supporting the upper waste liquid collecting box; the horizontal vibration motor head is connected with the side surface of the upper waste liquid collecting box. The utility model discloses an escherichia coli engineering bacteria zymotic fluid is collected in accumulation tank, uses the peristaltic pump slowly with the zymotic fluid pump to the filter pulp on, vibrating motor makes the filter pulp shake slowly, makes escherichia coli cell enrich on the filter pulp, and operating condition is mild, realizes going up appearance in succession.
Description
Technical Field
The utility model belongs to the technical field of the cell collection technique and specifically relates to an escherichia coli engineering bacteria collection device in zymotic fluid is related to in the cell collection field.
Background
The fermentation of engineering bacteria of Escherichia coli is a common technical path for producing microbial products, and different collection means, specifically collecting cells or collecting extracellular clear liquid, are selected intracellularly or extracellularly according to the distribution condition of target substances.
In the prior art, solid-liquid separation equipment is often selected to treat fermentation liquor for the intracellular condition of a target substance so as to rapidly collect cells. Therefore, on the basis of improving the production efficiency, the equipment has the characteristics of rapidness, mild conditions, capability of avoiding cell breakage and target object loss, convenience in operation and easiness in collection.
Therefore, the common practice in industrial production is to adopt a disk centrifuge as solid-liquid separation equipment, and the single treatment capacity is large, but the disadvantages are that the space occupation is large, the operation cost is high, the maintenance procedure is complicated, the treatment process is easy to cause the condition of cell disruption in a large amount, the method is not suitable for the requirement of small amount of rapid collection, and the problem that continuous sample loading cannot be realized exists.
Disclosure of Invention
In view of the above shortcomings in the prior art, the present invention provides an engineering bacteria collecting device for escherichia coli, which is used to solve the difficulties in the prior art.
In order to realize above-mentioned purpose and other relevant purpose, the utility model provides an escherichia coli engineering bacteria collection device, include:
the top of the upper waste liquid collecting box 1 receives fermentation liquid pumped out from a fermentation liquid accumulation tank 2 through a peristaltic pump 3, and the bottom of the upper waste liquid collecting box 1 is provided with a fermentation liquid outlet 4;
the filtering unit 5 is arranged in the upper waste liquid collecting box 1, the bottom of the filtering unit 5 is positioned above the fermentation liquid outlet 4, the top of the filtering unit 5 is positioned below the inflow of the fermentation liquid, and a spring 6 is arranged between the filtering unit 5 and the inner wall of the upper waste liquid collecting box 1;
the top of the lower waste liquid collecting box 7 is open, the bottom of the lower waste liquid collecting box 7 is closed, a cavity for receiving liquid flowing out from the bottom of the filtering unit 5 is formed inside the lower waste liquid collecting box 7, and the side wall of the lower waste liquid collecting box 7 positioned inside the lower waste liquid collecting box also extends horizontally towards the inner side integrally to form a supporting surface structure 8 for supporting the upper waste liquid collecting box 1;
and the horizontal vibration motor 9 is arranged on the lower waste liquid collecting box 7, and a motor head is connected with the side surface of the upper waste liquid collecting box 1.
According to a preferred solution, the size of the through hole formed inside the supporting surface structure 8 is smaller than the size of the bottom of the upper waste collection tank 1.
According to a preferred solution, the filtering unit 5 comprises:
a hard frame 51, wherein springs 6 are arranged on two side surfaces of the hard frame 51, which are close to the same axis of the horizontal vibration motor 9, on the same axis;
the bottom of the hard frame 51 is provided with filter meshes 52, and the inner diameter of each filter mesh 52 is larger than that of each filter cotton 53;
and the filter cotton 53, wherein the filter cotton 53 is attached to the bottom surface of the hard frame 51 in a replaceable way.
According to a preferable scheme, the material of the hard frame 51 is metal, durable material or light polymer material.
According to the preferred scheme, the filter cotton 53 can be manually disassembled and assembled.
According to the preferred scheme, the filter cotton 53 is wave filter cotton, and the direction of the waves is the same as the vibration direction of the horizontal vibration motor 9.
According to the preferred scheme, the top of the upper waste liquid collecting box 1 is also provided with a top cover 11, and a liquid inlet 12 is formed in the top cover 11 above the filtering unit 5.
According to a preferred solution, the size of the liquid inlet 12 is smaller than the size of the top opening of the upper waste liquid collecting tank 1.
Preferably, the width of the bottom of the inlet 12 is less than the width of the top.
According to the preferred scheme, the height of the upper waste liquid collecting box 1 is larger than that of the filter cotton 53.
According to the preferred scheme, a liquid outlet 13 is arranged on one side of the bottom of the lower waste liquid collecting box 7, and a liquid discharge valve 14 is installed on the liquid outlet 13.
According to the preferred scheme, a cavity for collecting waste liquid in the lower waste liquid collecting box 7 is internally provided with a liquid guide platform 10 which is arranged close to the liquid outlet 13 and has gradually reduced height.
The utility model discloses an go up the waste liquid collecting box, the filter unit, lower waste liquid collecting box and horizontal vibration motor, it gathers in the accumulation jar to have realized escherichia coli engineering bacteria zymotic fluid, use the peristaltic pump slowly with the zymotic fluid pump to filter pulp on, open the shock motor, make filter pulp slowly shake, promote and filter, finally make escherichia coli cell enrich on filter pulp, after the operation is accomplished, open the flowing back valve, with waste liquid exhaust effect, and operating condition is mild, be difficult for causing the cell breakage, it is convenient to maintain, can realize going up appearance in succession.
The following description of the preferred embodiments for carrying out the present invention will be made in detail with reference to the accompanying drawings so that the features and advantages of the present invention can be easily understood.
Drawings
Fig. 1 is a schematic perspective view of the present invention;
FIG. 2 is a view showing an internal structure of the present invention;
FIG. 3 is an enlarged schematic view of the upper waste liquid collecting box of the present invention;
FIG. 4 is an enlarged schematic view of the upper waste liquid collecting box of the present invention from another perspective;
FIG. 5 is a schematic view of the structure of the present invention;
description of the reference symbols
1. An upper waste liquid collecting box; 2. a fermentation liquor accumulation tank; 3. a peristaltic pump; 4. a fermentation liquor outlet; 5. a filtering unit 51, a hard frame 52, a filtering mesh 53 and filtering cotton; 6. a spring; 7. a lower waste liquid collection box; 8. a support surface structure; 9. a horizontal vibration motor; 10. a liquid guide table; 11. a top cover; 12. a liquid inlet; 13. a liquid outlet; 14. and a liquid discharge valve.
Detailed Description
In order to make the technical solution of the present invention's purpose, technical solution and advantages clearer, the following description will be combined with the drawings of the specific embodiments of the present invention, to carry out clear and complete description on the technical solution of the embodiments of the present invention. Like reference symbols in the various drawings indicate like elements. It should be noted that the described embodiments are some, not all embodiments of the present invention. Based on the described embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.
Possible embodiments within the scope of the invention may have fewer parts, have other parts not shown in the figures, different parts, differently arranged parts or differently connected parts, etc. than the examples shown in the figures. In addition, two or more of the components in the drawings may be implemented in a single component, or a single component shown in the drawings may be implemented as multiple separate components.
Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs. The use of "first," "second," and similar terms in the description and in the claims does not indicate any order, quantity, or importance, but rather is used to distinguish one element from another. Also, the use of the terms "a" or "an" and the like do not necessarily denote a limitation of quantity. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used only to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
The utility model provides an escherichia coli engineering fungus collection device for in the technology of collecting the bacterial in the liquid fermentation liquid, the utility model discloses do not restrict vibrating device's type, but in this collection device's structure specially adapted escherichia coli engineering fungus zymotic fluid.
Generally, the utility model provides an escherichia coli engineering bacteria collection device mainly includes waste liquid collecting box 1, filter unit 5, lower waste liquid collecting box 7 and horizontal vibration motor 9. Among them, reference may be made to fig. 1, which shows the arrangement relationship of an upper waste liquid collection tank 1, a filter unit 5, a lower waste liquid collection tank 7, and a horizontal vibration motor 9.
In order to realize the purpose of automatically collecting cells in escherichia coli engineering bacteria fermentation broth and solve the problem of high starting cost of opening experimental equipment due to small manual shaking in the background technology, in the technical scheme provided by the embodiment, an upper waste liquid collecting box 1 and a lower waste liquid collecting box 7 which are arranged in an up-and-down stacked mode are arranged, the upper waste liquid collecting box 1 can shake under the action of a horizontal vibrating motor 9, the cost problem and the labor problem are avoided, and the collection efficiency is improved.
It is specific, go up waste liquid collecting box 1 and set up the top at waste liquid collecting box 7 down, 7 open-top of lower waste liquid collecting box, the bottom is sealed, in order to load on waste liquid collecting box 1, down waste liquid collecting box 7 correspond on the inside wall an organic whole towards inboard horizontal extension formation support face structure 8 of waste liquid collecting box 1, make and go up waste liquid collecting box 1 and can remove and can not drop along the removal about face structure 8, consequently the inside through-hole size that forms of face structure 8 is less than the bottom size of waste liquid collecting box 1.
As shown in fig. 1, the top of the upper waste liquid collecting tank 1 receives the fermentation liquid pumped out from the fermentation liquid accumulation tank 2 through the peristaltic pump 3, the rotation speed of the peristaltic pump is adjusted to enable the fermentation liquid to be filtered out from the filter cotton gently without overflowing, the bottom of the upper waste liquid collecting tank is provided with a fermentation liquid outlet 4, the fermentation liquid outlet 4 is located above a through hole formed in the supporting surface structure 8, wherein the upper waste liquid collecting tank 1 is a structure mainly bearing filtering action, a filtering unit 5 is loaded in the upper waste liquid collecting tank, the bottom of the upper waste liquid collecting tank is provided with a fermentation liquid outlet 4, and the liquid flowing out from the fermentation liquid outlet 4 flows into the lower waste liquid collecting tank 7 to play a role in bearing the filtered liquid.
As shown in fig. 2, 3 and 4, regarding the filtering unit 5, the upper waste liquid collecting box 1 is placed in the hard frame 51, the filtering cotton 53 which can be manually disassembled and assembled is placed in the hard frame 51, because of the vibration filtering, the filtering cotton 53 is a wave type filtering cotton, the bottom of the hard frame 51 is provided with a filtering mesh 52 which is positioned below the filtering cotton 53, wherein the inner diameter of the filtering mesh 52 is larger than that of the filtering cotton 53; next, a spring 6 is arranged between the hard frame 51 and the inner wall of the upper waste liquid collecting box 1, and because the horizontal vibrating motor 9 is arranged on the lower waste liquid collecting box 7, and the motor head is connected with the side surface of the upper waste liquid collecting box 1, the hard frame 51 can be driven to vibrate under the driving of the horizontal vibrating motor 9 to accelerate the filtering of the fermentation liquid, and the principle of the device is similar to that of a vibration table; further, in order to improve the vibration strength of the hard frame 51, the hard frame 51 is generally made of metal, durable material or light polymer material, so as to reduce the influence of the weight on the vibration strength.
It should be noted that the filter cotton 53 is placed on the hard frame 51 for filtering the fermentation liquid to collect the cells; after the cells are collected, the filter cotton 53 is taken down, and the cells can be washed from the filter cotton 53 according to different production requirements, so that the filter cotton 53 can be reused; or the used filter cotton can be discarded and directly replaced by new filter cotton.
As described above, since the filtered liquid falls straight from the top of the filter unit 5, it is necessary to consider whether the liquid will spill when reaching the filter unit 5, on one hand, the top cover 11 is installed on the top of the upper waste liquid collecting tank 1, the liquid inlet 12 is opened on the top cover 11 above the filter unit 5, and since the size of the liquid inlet 12 is smaller than the size of the top opening of the upper waste liquid collecting tank 1 and the bottom width of the liquid inlet 12 is smaller than the top width, the liquid inlet range can be effectively reduced, that is, the liquid inlet 12 is located in the hard frame 51 when the liquid inlet 12 and the hard frame 51 are viewed from above;
on the other hand, the height of the upper waste liquid collecting box 1 is larger than that of the filter cotton 53, so that the side wall can be used for blocking splashed liquid.
As shown in fig. 1 and 2, a cavity for receiving the liquid flowing out from the bottom of the filtering unit 5 is formed in the lower waste liquid collecting tank 7, a liquid guiding table 10 with gradually reduced height is formed in the cavity for collecting the waste liquid in the lower waste liquid collecting tank 7, the liquid guiding table is arranged close to the liquid outlet 13, a liquid outlet 13 is arranged on one side of the liquid guiding table, and the purpose of discharging the waste liquid is achieved through a liquid discharging valve 14 on the liquid outlet 13.
The above embodiments are merely illustrative of the principles and effects of the present invention, and are not to be construed as limiting the invention. Any person skilled in the art can modify or change the above embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (7)
1. The utility model provides an escherichia coli engineering bacteria collection device which characterized in that includes:
the top of the upper waste liquid collecting box (1) receives fermentation liquid pumped out from the fermentation liquid accumulation tank (2) through the peristaltic pump (3), and the bottom of the upper waste liquid collecting box (1) is provided with a fermentation liquid outlet (4);
the filter unit (5) is arranged in the upper waste liquid collecting box (1), the bottom of the filter unit (5) is positioned above the fermentation liquid outlet (4), the top of the filter unit is positioned below the inflow of the fermentation liquid, and a spring (6) is arranged between the filter unit (5) and the inner wall of the upper waste liquid collecting box (1);
the top of the lower waste liquid collecting box (7) is open, the bottom of the lower waste liquid collecting box (7) is closed, a cavity for receiving liquid flowing out from the bottom of the filtering unit (5) is formed inside the lower waste liquid collecting box, and the side wall of the lower waste liquid collecting box (7) positioned inside the lower waste liquid collecting box is also integrally and horizontally extended towards the inner side to form a supporting surface structure (8) for supporting the upper waste liquid collecting box (1);
the horizontal vibration motor (9), horizontal vibration motor (9) are installed on lower waste liquid collecting box (7), and the motor head is connected with the side of last waste liquid collecting box (1).
2. The Escherichia coli engineering bacteria collection device according to claim 1, wherein the filter unit (5) comprises:
the spring (6) is arranged on two side faces, close to the same axis, of the hard frame (51), of the horizontal vibration motor (9);
the bottom of the hard frame (51) is provided with filter meshes (52), and the inner diameter of each filter mesh (52) is larger than that of each filter cotton (53);
the filter cotton (53), the filter cotton (53) is attached to the bottom surface of the hard frame (51) in a replaceable way.
3. The Escherichia coli engineering bacteria collection device according to claim 2, wherein the filter cotton (53) is wave filter cotton, and the direction of the wave is the same as the vibration direction of the horizontal vibration motor (9).
4. The escherichia coli engineering bacteria collection device according to claim 3, wherein a top cover (11) is further mounted at the top of the upper waste liquid collection box (1), and a liquid inlet (12) is formed in the position, above the filter unit (5), of the top cover (11).
5. The Escherichia coli engineering bacteria collection device according to claim 4, wherein the height of the upper waste liquid collection box (1) is larger than that of the filter cotton (53).
6. The Escherichia coli engineering bacteria collection device according to claim 5, wherein a liquid outlet (13) is arranged at one side of the bottom of the lower waste liquid collection box (7), and a liquid discharge valve (14) is mounted on the liquid outlet (13).
7. The Escherichia coli engineering bacteria collection device according to claim 6, wherein a liquid guide platform (10) with gradually reduced height is formed in the cavity for collecting waste liquid in the lower waste liquid collection box (7) and is arranged close to the liquid outlet (13).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222928526.9U CN218811709U (en) | 2022-11-02 | 2022-11-02 | Escherichia coli engineering bacteria collecting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222928526.9U CN218811709U (en) | 2022-11-02 | 2022-11-02 | Escherichia coli engineering bacteria collecting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218811709U true CN218811709U (en) | 2023-04-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222928526.9U Active CN218811709U (en) | 2022-11-02 | 2022-11-02 | Escherichia coli engineering bacteria collecting device |
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| CN (1) | CN218811709U (en) |
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- 2022-11-02 CN CN202222928526.9U patent/CN218811709U/en active Active
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