CN218811707U - Quick filter equipment of post is extracted to plasmid - Google Patents
Quick filter equipment of post is extracted to plasmid Download PDFInfo
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- CN218811707U CN218811707U CN202221355838.9U CN202221355838U CN218811707U CN 218811707 U CN218811707 U CN 218811707U CN 202221355838 U CN202221355838 U CN 202221355838U CN 218811707 U CN218811707 U CN 218811707U
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- negative pressure
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- plasmid extraction
- plasmid
- extraction column
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Abstract
The utility model discloses a rapid filtering device of a plasmid extraction column, which comprises a base, a negative pressure connecting pipe, a threaded connecting piece and a negative pressure generating assembly; the negative pressure connecting pipe is arranged on the base and is connected with the negative pressure generating assembly through a conduit; the threaded connecting piece is arranged on the negative pressure connecting pipe in a threaded manner, and threaded holes matched with plasmid extraction columns with different diameters are formed in the top end of the threaded connecting piece; the negative pressure generating assembly is connected with the negative pressure connecting pipe through a conduit and is used for providing a negative pressure environment. The utility model discloses a detachable structure has realized the plasmid of different models and has extracted quick installation and the dismantlement of post, has improved the filter efficiency that the post was extracted to the plasmid greatly, has taken the receiving flask to carry out the collection of liquid composition in addition the filter process, has avoided liquid composition to enter into the vacuum pump effectively in, has guaranteed the stability of overall structure operation.
Description
Technical Field
The utility model relates to a biological treatment field especially relates to a quick filter equipment of post is extracted to plasmid.
Background
The plasmid extraction method is to remove RNA, separate the plasmid from the bacterial genomic DNA, and remove proteins and other impurities to obtain relatively pure plasmid. The methods for extracting plasmid DNA are various, and the extraction yield can be divided into micro extraction, medium extraction and large extraction, the extraction yield can be divided into general extraction and kit extraction, the specific operation methods can be divided into alkaline lysis method, boiling method, toothpick method and the like, various methods have advantages and disadvantages, and the proper extraction method can be adopted according to different experimental purposes.
In the process of extracting DNA from plasmids, liquid components such as deionized water and the like need to be filtered, and the filtering device in the prior art can not carry out filtering operation on plasmid extraction columns with different models and sizes, and has certain defects.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a plasmid extraction post rapid filtration device can filter the plasmid extraction post of different models size, can also guarantee the stability of overall structure operation simultaneously.
In order to solve the technical problem, the utility model provides a fast filtering device of a plasmid extraction column, which comprises a base, a negative pressure connecting pipe, a threaded connecting piece and a negative pressure generating assembly;
the negative pressure connecting pipe is arranged on the base and is connected with the negative pressure generating assembly through a conduit;
the threaded connecting piece is arranged on the negative pressure connecting pipe in a threaded manner, and threaded holes matched with plasmid extraction columns with different diameters are formed in the top end of the threaded connecting piece;
the negative pressure generating assembly is connected with the negative pressure connecting pipe through a conduit and is used for providing a negative pressure environment.
Furthermore, a switch valve is arranged in the middle of the threaded connecting piece.
Furthermore, the joints of the two ends of the threaded connecting piece are provided with sealing gaskets.
Further, the negative pressure generating assembly comprises a collecting bottle, an air inlet pipe and an air exhaust pipe are arranged on the collecting bottle, the air inlet pipe is connected with the conduit, and the air exhaust pipe is connected with a vacuum pump.
Furthermore, the bottom end of the air inlet pipe extends into the bottom in the collecting bottle.
Further, the bottom end of the air inlet pipe is arranged to be pointed.
Further, the inner end of the exhaust tube is positioned at the top in the collecting bottle.
Compared with the prior art, the utility model discloses following beneficial effect has at least:
the utility model discloses a detachable structure has realized the plasmid of different models and has extracted the quick installation and the dismantlement of post, has improved the filtration efficiency that the post was extracted to the plasmid greatly, has taken the receiving flask to carry out the collection of liquid composition in addition the filtration process, has avoided the liquid composition to enter into the vacuum pump effectively in, has guaranteed the stability of overall structure operation.
Drawings
FIG. 1 is a schematic view of the overall structure of one embodiment of the rapid filtering device for plasmid extraction column of the present invention;
FIG. 2 is a schematic view of the structure of the threaded connection piece of one embodiment of the rapid filtration device for plasmid extraction column of the present invention.
Detailed Description
The plasmid extraction column rapid filtration device of the present invention will now be described in greater detail with reference to the schematic drawings, in which preferred embodiments of the present invention are shown, it being understood that those skilled in the art can modify the invention described herein while still achieving the advantageous effects of the present invention. Accordingly, the following description should be construed as broadly as possible to those skilled in the art and not as limiting the invention.
The invention is described in more detail in the following paragraphs by way of example with reference to the accompanying drawings. The advantages and features of the present invention will become more fully apparent from the following description and appended claims. It should be noted that the drawings are in simplified form and are not to precise scale, and are provided for convenience and clarity in order to facilitate the description of the embodiments of the present invention.
As shown in fig. 1 and fig. 2, an embodiment of the present invention provides a rapid filtration device for a plasmid extraction column, which includes a base 1, a negative pressure connection pipe 2, a threaded connection member 3, and a negative pressure generation assembly;
the negative pressure connecting pipe 2 is arranged on the base 1, and the negative pressure connecting pipe 2 is connected with the negative pressure generating assembly through a conduit 5;
the threaded connecting piece 3 is arranged on the negative pressure connecting pipe 2 in a threaded manner, and the top end of the threaded connecting piece 3 is provided with threaded holes 4 matched with plasmid extraction columns 12 with different diameters;
and the negative pressure generating assembly is used for providing a negative pressure environment.
In this embodiment, when the plasmid extraction column 12 needs to be filtered, the threaded connectors 3 of different models are selected according to the diameter of the plasmid extraction column 12 and screwed into the negative pressure connecting pipe 2, and then the plasmid extraction column 12 is screwed into the threaded hole 4 at the top of the threaded connector 3, the negative pressure generating assembly is opened, and the liquid component of the plasmid extraction column 12 is filtered into the negative pressure generating assembly by using the negative pressure for temporary storage, so that the filtering operation of the plasmid extraction columns 12 of different sizes is realized.
The following examples of the plasmid extraction column rapid filtration device are presented to clearly illustrate the contents of the present invention, and it should be understood that the contents of the present invention are not limited to the following examples, and other modifications by conventional technical means of those skilled in the art are within the scope of the idea of the present invention.
And a switch valve 6 is arranged in the middle of the threaded connecting piece 3. In this embodiment, the setting of the switch valve 6 can be selected to open or close according to the actual use requirement, so as to ensure the stable maintenance of the negative pressure environment in the negative pressure connecting pipe 3, and further ensure the smooth operation of the filtration operation of the plasmid extraction column 12.
And sealing gaskets 7 are arranged at the joints of the two ends of the threaded connecting piece 3. In this embodiment, the bottom end of the threaded connector 3 is an external thread, and the top end is an internal thread, so as to realize the quick connection between the threaded connector 3 and the negative pressure connecting pipe 2 and the plasmid extraction column 12. When the threaded connecting piece 3 and the plasmid extraction column 12 are installed, the sealing gasket 7 is added, so that the whole air tightness is ensured, and the stable operation of the filtering operation of the plasmid extraction column 12 is realized.
The negative pressure generating assembly comprises a collecting bottle 8, an air inlet pipe 9 and an air exhaust pipe 10 are arranged on the collecting bottle 8, the air inlet pipe 9 is connected with the catheter 5, and the air exhaust pipe 10 is connected with a vacuum pump 13. In this embodiment, a vacuum pump 13 is used to pump out the air in the collection bottle 8 to form a negative pressure environment, and the filtration operation of the plasmid extraction column 12 is realized by the negative pressure.
The bottom end of the air inlet pipe 9 extends into the bottom of the collecting bottle 8. In this embodiment, the bottom end of the air inlet pipe 8 extends into the bottom of the collection bottle 8, so that splashing caused by the liquid components filtered in the plasmid extraction column 12 falling into the collection bottle 8 can be avoided, and the liquid components are effectively prevented from being sucked into the vacuum pump 13.
The bottom end of the air inlet pipe 9 is arranged to be pointed. In this embodiment, the bottom end of the air inlet tube 9 is pointed, so that a gap can be formed between the air inlet tube 9 and the inner bottom surface of the collection bottle 8, thereby ensuring that the liquid components filtered in the plasmid extraction column 12 smoothly enter the collection bottle 8.
The inner end of the air suction pipe 10 is positioned at the top in the collecting bottle 8. In this embodiment, the inner end of the air extraction tube 10 is located at the top of the collection bottle 8, and has a certain height difference with the air inlet tube 9, so as to effectively prevent the liquid components filtered in the plasmid extraction column 12 from being sucked into the vacuum pump 13.
To sum up, the utility model discloses a detachable structure has realized the plasmid of different models and has extracted the quick installation and the dismantlement of post, has improved the filter efficiency that the post was extracted to the plasmid greatly, has taken the receiving flask to carry out the collection of liquid composition in addition the filter process, has avoided liquid composition to enter into the vacuum pump effectively in, has guaranteed the stability of overall structure operation.
It will be apparent to those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (7)
1. A rapid filtering device of a plasmid extraction column is characterized by comprising a base, a negative pressure connecting pipe, a threaded connecting piece and a negative pressure generating assembly;
the negative pressure connecting pipe is arranged on the base and is connected with the negative pressure generating assembly through a conduit;
the threaded connecting piece is arranged on the negative pressure connecting pipe in a threaded manner, and threaded holes matched with plasmid extraction columns with different diameters are formed in the top end of the threaded connecting piece;
the negative pressure generating assembly is connected with the negative pressure connecting pipe through a conduit and is used for providing a negative pressure environment.
2. The plasmid extraction column rapid filtration device of claim 1, wherein a switch valve is arranged in the middle of the threaded connector.
3. The plasmid extraction column rapid filtration device of claim 1, wherein the joints of both ends of the threaded connector are provided with sealing gaskets.
4. The plasmid extraction column rapid filtering device of claim 1, wherein the negative pressure generating assembly comprises a collection bottle, the collection bottle is provided with an air inlet pipe and an air exhaust pipe, the air inlet pipe is connected with the conduit, and the air exhaust pipe is connected with a vacuum pump.
5. The plasmid extraction column rapid filtration device of claim 4, wherein the bottom end of the air inlet pipe extends into the bottom of the collection bottle.
6. The plasmid extraction column rapid filtration device of claim 4, wherein the bottom end of the air inlet pipe is provided in a pointed shape.
7. The plasmid extraction column rapid filtration device of claim 4, wherein the inner end of the suction tube is positioned at the top inside the collection bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221355838.9U CN218811707U (en) | 2022-06-01 | 2022-06-01 | Quick filter equipment of post is extracted to plasmid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221355838.9U CN218811707U (en) | 2022-06-01 | 2022-06-01 | Quick filter equipment of post is extracted to plasmid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218811707U true CN218811707U (en) | 2023-04-07 |
Family
ID=87252006
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221355838.9U Active CN218811707U (en) | 2022-06-01 | 2022-06-01 | Quick filter equipment of post is extracted to plasmid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218811707U (en) |
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2022
- 2022-06-01 CN CN202221355838.9U patent/CN218811707U/en active Active
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