CN218728316U - Multi-wavelength laser light source confocal microscope using diffraction grating - Google Patents
Multi-wavelength laser light source confocal microscope using diffraction grating Download PDFInfo
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- CN218728316U CN218728316U CN202223175031.XU CN202223175031U CN218728316U CN 218728316 U CN218728316 U CN 218728316U CN 202223175031 U CN202223175031 U CN 202223175031U CN 218728316 U CN218728316 U CN 218728316U
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Abstract
The utility model discloses an use multi-wavelength laser light source confocal microscope of diffraction grating, including light source module, fiber collimator, speculum, diffraction grating component, focusing lens, wave filter, dichroic mirror, scanning module, microscope objective, light filter and detector, light source module, fiber collimator, speculum, diffraction grating component, focusing lens, wave filter, dichroic mirror, scanning module, microscope objective set gradually along laser incident direction, and microscope objective, dichroic mirror, light filter, detector set gradually along fluorescence incident direction. The utility model discloses in, the light source module is closed by the laser instrument of a plurality of different wavelengths and is restrainted and form, closes and restraints light and incides to the diffraction grating component after optical collimator and speculum collimate and reflect, and through utilizing the diffraction grating component to replace optical filter to filter, the light beam of different wavelengths can part the outgoing each other, and diffraction efficiency is higher, and the optical power loss is little, and overall structure volume is less, is fit for integrating the design.
Description
Technical Field
The utility model belongs to the technical field of the microscope, concretely relates to use multi-wavelength laser light source confocal microscope of diffraction grating.
Background
A confocal laser microscope is an apparatus for molecular and cell biology research, and is mainly characterized in that a laser scanning beam is utilized to form a point light source through a grating pinhole, the point light source scans point by point on a focal plane of a fluorescence-labeled specimen, an optical signal of an acquisition point reaches a detector through a detection pinhole, and an image is formed on a computer monitoring screen after signal processing. In the existing laser confocal microscope, the light source emission end filters out the unwanted excitation wavelength mostly through an optical filter, the transmittance of the optical filter is often influenced by a coating process and an incident angle, the filtering capability is limited, and the unwanted excitation wavelength can be filtered out only by overlapping a plurality of optical filters, so that the cost is increased, and the assembly precision is also improved.
SUMMERY OF THE UTILITY MODEL
For solving the technical problem that exists among the prior art, the utility model aims to provide an use multi-wavelength laser light source confocal microscope of diffraction grating.
In order to realize the above purpose, reach above-mentioned technological effect, the utility model discloses a technical scheme be:
the light source module, the optical fiber collimator, the reflecting mirror, the diffraction grating element, the focusing lens, the filter, the dichroic mirror, the scanning module, the microscope objective, the optical filter and the detector are sequentially arranged along a laser incidence direction, and the microscope objective, the dichroic mirror, the optical filter and the detector are sequentially arranged along a fluorescence incidence direction.
The utility model provides a pair of use multi-wavelength laser light source confocal microscope of diffraction grating, the speculum includes speculum one and speculum two, optical collimator, speculum one, speculum two set gradually along laser incident direction.
The utility model provides a pair of use multi-wavelength laser light source confocal microscope of diffraction grating, the light source module includes laser instrument group, collimating lens group, dichroic mirror group, two adhesive lens and the single mode fiber that set gradually along laser incidence direction, single mode fiber, fiber collimator, speculum set gradually along laser incidence direction, the light of laser instrument group transmission becomes a bunch of light through collimating lens group after the multiple beam collimated light is do not become through dichroic mirror combination one beam of light again, and the light beam after the beam combination advances single mode fiber through two adhesive lens couplings.
The utility model provides a pair of use multi-wavelength laser light source confocal microscope of diffraction grating, the diameter of facula after closing is 0.3mm ~ 6mm.
The utility model provides an in the multi-wavelength laser light source confocal microscope of an use diffraction grating, single mode fiber's fibre core is 3 mu m or 9 mu m, and numerical aperture is 0.1 ~ 0.12.
The utility model provides a pair of use multi-wavelength laser light source confocal microscope of diffraction grating, laser instrument group includes the laser instrument of the different wavelength of a plurality of, collimating lens group includes a plurality of collimating lens, dichroic mirror group includes a plurality of dichroic mirror, and every laser instrument sets up with a collimating lens and a dichroic mirror looks adaptation and one-to-one.
In the multi-wavelength laser light source confocal microscope using the diffraction grating, four lasers are arranged, which are sequentially marked as a first laser, a second laser, a third laser and a fourth laser from left to right, and the wavelength range is 375 nm-785 nm; the collimating lens group comprises four collimating lenses which are sequentially marked as a first collimating lens, a second collimating lens, a third collimating lens and a fourth collimating lens from left to right, and the collimating lenses can be aspheric lenses, spherical lenses or spherical lenses; the dichroic mirror group comprises four dichroic mirrors, and the four dichroic mirrors are sequentially marked as a first dichroic mirror, a second dichroic mirror, a third dichroic mirror and a fourth dichroic mirror from left to right; the three-phase laser device, the three-phase collimating lens and the three-phase dichroic mirror are adaptive and arranged in a one-to-one correspondence manner, and the four-phase laser device, the four-phase collimating lens and the four-phase dichroic mirror are adaptive and arranged in a one-to-one correspondence manner.
In the multi-wavelength laser source confocal microscope using the diffraction grating, the diffraction order of the diffraction grating element is +/-1, +/-2, the scale line number is 3600 Lines/mm-7200 Lines/mm, and the incident angle is-40 degrees.
The utility model provides an in the multi-wavelength laser light source confocal microscope of use diffraction grating, the wave filter is the pinhole filter, and its pinhole bore is 1 ~ 20 mu m.
The utility model provides a pair of use multi-wavelength laser light source confocal microscope of diffraction grating, microobjective's numerical aperture is 0.8 ~ 1.2, and the line field of view is 15mm ~ 23mm, and magnification is 4 ~ 100X, one side that scanning module was kept away from to microobjective is provided with the sample, and the fluorescence of different wavelengths is excited to the exciting light in the sample, and fluorescence is incited to the dichroic mirror through microobjective on, is incited to pass through the detector again on the filter by the fluorescence of dichroic mirror reflection and receives fluorescence signal.
Compared with the prior art, the beneficial effects of the utility model are that:
the utility model discloses an use multi-wavelength laser light source confocal microscope of diffraction grating, including light source module, fiber collimator, speculum, diffraction grating component, focusing lens, wave filter, dichroic mirror, scanning module, microscope objective, light filter and detector, light source module, fiber collimator, speculum, diffraction grating component, focusing lens, wave filter, dichroic mirror, scanning module, microscope objective set gradually along laser incident direction, and microscope objective, dichroic mirror, light filter, detector set gradually along fluorescence incident direction. The utility model discloses in, the light source module closes by the laser instrument of a plurality of different wavelengths and restraints and form, closes and restraints light and incides to the diffraction grating component after optical collimator and speculum are collimated and are reflected, filters through utilizing the diffraction grating component to replace optical filter, and the light beam of different wavelengths can part the outgoing each other, and diffraction efficiency is higher, and the optical power loss is little, and overall structure volume is less, is fit for integrating the design.
Drawings
Fig. 1 is a schematic structural view of the present invention;
fig. 2 is a schematic structural diagram of a light source module according to the present invention;
fig. 3 is a schematic structural diagram of the pinhole filter of the present invention;
FIG. 4 is a speckle pattern of different wavelengths obtained by the diffraction grating element of the present invention after beam splitting and focusing;
FIG. 5 is a graph of fluorescence signals obtained by the detector of the present invention.
Detailed Description
The present invention is described in detail below to enable the advantages and features of the present invention to be more easily understood by those skilled in the art, thereby making more clear and definite definitions of the scope of the present invention.
The following presents a simplified summary of one or more aspects in order to provide a basic understanding of such aspects. This summary is not an extensive overview of all contemplated aspects, and is intended to neither identify key or critical elements of all aspects nor delineate the scope of any or all aspects. Its sole purpose is to present some concepts of one or more aspects in a simplified form as a prelude to the more detailed description that is presented later.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Example 1
As shown in fig. 1 to 5, a multi-wavelength laser light source confocal microscope using a diffraction grating includes a light source module, an optical fiber collimator 12, a first reflector 13, a second reflector 14, a diffraction grating element 15, a focusing lens 16, a pinhole filter 17, a dichroic mirror five 18, a scanning module 19, a microscope objective 20, a filter 22, and a detector 23, where the light source module, the optical fiber collimator 12, the first reflector 13, the second reflector 14, the diffraction grating element 15, the focusing lens 16, the pinhole filter 17, the dichroic mirror five 18, the scanning module 19, and the microscope objective 20 are sequentially arranged along a laser incidence direction, a sample 21 is arranged on a side of the microscope objective 20 away from the scanning module 19, excitation light excites fluorescence of different wavelengths in the sample 21, and the microscope objective 20, the dichroic mirror five 18, the filter 22, and the detector 23 are sequentially arranged along the fluorescence incidence direction.
The light source module comprises a laser group, a collimating lens group 5, a dichroic lens group, a double-cemented lens 10 and a single-mode optical fiber 11, wherein the double-cemented lens 10 is used for coupling and achromatizing, the fiber core of the single-mode optical fiber 11 is 3 mu m or 9 mu m, and the numerical aperture is 0.1-0.12; the light emitted by the laser group is changed into a plurality of collimated lights through the collimating lens group 5 to be emitted, and then the collimated lights are combined into a beam of light through the dichroic mirror respectively, the diameter of the combined beam spot can be 0.3-6 mm, and the combined beam of light is coupled into a single-mode optical fiber 11 through a double-cemented lens 10.
In this embodiment, the laser group includes four lasers with different wavelengths, which are sequentially recorded as a first laser 1, a second laser 2, a third laser 3, and a fourth laser 4 from left to right, the wavelength usable range is 375nm to 785nm, light rays emitted by the four lasers are respectively changed into four collimated light beams by the collimating lens group 5 to be emitted, and then the four collimated light beams are respectively combined into one light beam by the dichroic mirror.
The collimating lens group 5 includes four collimating lenses, which are sequentially marked as a first collimating lens 51, a second collimating lens 52, a third collimating lens 53, and a fourth collimating lens 54 from left to right, and the collimating lens may be an aspheric lens, a spherical lens, or a spherical lens.
The dichroic mirror group comprises four dichroic mirrors, namely a dichroic mirror I6, a dichroic mirror II 7, a dichroic mirror III 8 and a dichroic mirror IV 9 which are sequentially marked from left to right.
The utility model provides an use diffraction grating's multi-wavelength laser light source confocal microscope's theory of operation does:
light emitted by the first laser 1 becomes collimated light to be emitted through the first collimating lens 51, is reflected by the first dichroic mirror 6, is transmitted out of the second dichroic mirror 7, and is then transmitted out of the third dichroic mirror 8 and the fourth dichroic mirror 9 in sequence, light emitted by the second laser 2 becomes collimated light to be emitted through the second collimating lens 52, is transmitted out of the third dichroic mirror 8 and the fourth dichroic mirror 9 in sequence after being reflected by the second dichroic mirror 7, light emitted by the third laser 3 becomes collimated light to be emitted through the third collimating lens 53, is transmitted out of the fourth dichroic mirror 9 after being reflected by the third dichroic mirror 8, light emitted by the fourth laser 4 becomes collimated light to be emitted through the fourth collimating lens 54, is reflected through the fourth dichroic mirror 9, and four beams of light are coupled into the single-mode optical fiber 11 through the double-adhesive lens 10;
then, after light is emitted through the single-mode fiber 11, the light is first changed into collimated light beams through a fiber collimator 12, the collimated light beams pass through a first reflector 13 and a second reflector 14 to adjust angles, the first reflector 13 and the second reflector 14 are respectively controlled by a motor M, the incident angle is determined by design parameters of a diffraction grating element 15, when the collimated light beams with a certain angle are incident on the diffraction grating element 15, light beams with different wavelengths are emitted separately, as shown in fig. 4, the angle of the emitted light beams is determined by design parameters of the diffraction grating element 15, the light beams split by the diffraction grating element 15 are incident on a focusing lens 16, the focusing lens 16 is an aspheric lens with a clear aperture which ensures that all the split light beams can be received, the light beams converged by the focusing lens 16, namely, a pinhole filter 17 is arranged at an image point of an imaging, other light beams which are not needed can be filtered by the pinhole filter 17, as shown in fig. 3, the aperture of each pinhole filter 17 can be 1-20 μ M, the distance between different pinholes of the different wavelengths of the pinhole filter 17 is matched with the wavelength of an excitation light beam and the excitation light beam from the diffraction grating element 15, the excitation light beam passes through a dichroic scanning microscope 20, the excitation lens 18, the excitation light beam is filtered by a scanning microscope system, the excitation light beam 18, the excitation light beam which is not capable of passing through a sample with a high-scanning microscope scanning tolerance, the scanning microscope 18, the excitation light beam which is required for detecting the scanning precision, the sample 18, the sample with the scanning precision, due to the wavelength selectivity of the dichroic mirror, the fluorescence will be emitted without being transmitted, but the dichroic mirror five 18 will also reflect a small portion of the excitation light reflected from the sample 21, because the film coated by the dichroic mirror five 18 cannot have a transmittance of 100%, and therefore, the reflected fluorescence must be incident on the filter 22 to filter the reflected excitation light, and finally the detector 23 receives the fluorescence signal, as shown in fig. 5. Because the fluorescence signal tends to be weak, the detector 23 should typically use a photomultiplier tube (PMT) arrangement to amplify the fluorescence signal.
In this embodiment, the design parameters of the diffraction grating element 15 generally include the diffraction order, the number of rulings, and the incident angle, the diffraction order of the diffraction grating element 15 can be ± 1 and ± 2, the number of the rulings is 3600Lines/mm to 7200Lines/mm, the incident angle is-40 ° to 40 °, and the manufacturing material of the diffraction grating element 15 is glass.
The numerical aperture of the microscope objective lens 20 is 0.8-1.2, the linear view field is 15-23 mm, and the magnification is 4-100X.
The utility model discloses the part or the structure that do not specifically describe adopt prior art or current product can, do not do here and describe repeatedly.
The above-mentioned only be the embodiment of the utility model discloses a not consequently restriction the patent scope of the utility model, all utilize the equivalent structure or the equivalent flow transform of what the specification content was done, or directly or indirectly use in other relevant technical field, all the same reason is included in the patent protection scope of the utility model.
Claims (10)
1. The multi-wavelength laser light source confocal microscope using the diffraction grating is characterized by comprising a light source module, an optical fiber collimator, a reflecting mirror, a diffraction grating element, a focusing lens, a filter, a dichroic mirror, a scanning module, a microscope objective, an optical filter and a detector, wherein the light source module, the optical fiber collimator, the reflecting mirror, the diffraction grating element, the focusing lens, the filter, the dichroic mirror, the scanning module and the microscope objective are sequentially arranged along a laser incidence direction, and the microscope objective, the dichroic mirror, the optical filter and the detector are sequentially arranged along a fluorescence incidence direction.
2. The confocal microscope of claim 1, wherein the reflector comprises a first reflector and a second reflector, and the fiber collimator, the first reflector and the second reflector are sequentially arranged along the incident direction of the laser.
3. The multi-wavelength laser light source confocal microscope with the diffraction grating as claimed in claim 1, wherein the light source module comprises a laser group, a collimating lens group, a dichroic lens group, a double cemented lens and a single mode fiber, which are arranged in sequence along the laser incidence direction, and the single mode fiber, the fiber collimator and the reflector are arranged in sequence along the laser incidence direction.
4. The confocal microscope of claim 3, wherein the diameter of the combined spot is 0.3mm to 6mm.
5. The confocal microscope of claim 3, wherein the single-mode fiber has a core of 3 μm or 9 μm and a numerical aperture of 0.1-0.12.
6. The confocal microscope of claim 3, wherein the set of lasers comprises a plurality of lasers with different wavelengths, the collimating lens set comprises a plurality of collimating lenses, the dichroic mirror set comprises a plurality of dichroic mirrors, and each laser is adapted to and arranged in a one-to-one correspondence with one collimating lens and one dichroic mirror.
7. The multi-wavelength laser light source confocal microscope using the diffraction grating as claimed in claim 6, wherein there are four lasers, which are sequentially marked as a first laser, a second laser, a third laser and a fourth laser from left to right, and the wavelength range is 375nm to 785nm; the collimating lens group comprises four collimating lenses, which are sequentially marked as a first collimating lens, a second collimating lens, a third collimating lens and a fourth collimating lens from left to right, and the collimating lenses can be aspheric lenses, spherical lenses or spherical lenses; the dichroic mirror group comprises four dichroic mirrors, and the four dichroic mirrors are sequentially marked as a first dichroic mirror, a second dichroic mirror, a third dichroic mirror and a fourth dichroic mirror from left to right; the three-phase laser device, the three-phase collimating lens and the three-phase dichroic mirror are adaptive and arranged in a one-to-one correspondence manner, and the four-phase laser device, the four-phase collimating lens and the four-phase dichroic mirror are adaptive and arranged in a one-to-one correspondence manner.
8. The confocal microscope of claim 1, wherein the diffraction grating element has a diffraction order of ± 1, ± 2, a scale of 3600Lines/mm to 7200Lines/mm, and an incident angle of-40 ° to 40 °.
9. The confocal microscope of claim 1, wherein the filter is a pinhole filter having a pinhole aperture of 1-20 μm.
10. The confocal microscope of claim 1, wherein the micro objective has a numerical aperture of 0.8-1.2, a linear field of view of 15-23 mm, and a magnification of 4-100X, a sample is disposed on a side of the micro objective away from the scanning module, the excitation light excites fluorescence with different wavelengths in the sample, the fluorescence is incident on the dichroic mirror through the micro objective, and the fluorescence reflected by the dichroic mirror is incident on the optical filter and receives a fluorescence signal through the detector.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116712020A (en) * | 2023-07-05 | 2023-09-08 | 苏州璞煜科技服务有限公司 | Multispectral cold light source system for medical endoscope |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116712020A (en) * | 2023-07-05 | 2023-09-08 | 苏州璞煜科技服务有限公司 | Multispectral cold light source system for medical endoscope |
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