CN218710304U

CN218710304U - Gene sequencing library preparation facilities

Info

Publication number: CN218710304U
Application number: CN202221624246.2U
Authority: CN
Inventors: 洪志强; 曹震廷; 王兆松; 郭小建
Original assignee: Shanghai 3D Medicines Co Ltd
Current assignee: Shanghai 3D Medicines Co Ltd
Priority date: 2022-06-27
Filing date: 2022-06-27
Publication date: 2023-03-24
Anticipated expiration: 2032-06-27

Abstract

The embodiment of the utility model provides a gene sequencing library preparation facilities, include: the card box integration piece comprises a card box bracket and a card box, wherein the card box bracket is provided with a card box installation groove suitable for installing the card box; the fluorescence module comprises a fluorescence quantitative detection part which can move relative to the card box integration part and is suitable for detecting the concentration of the experimental sample in the card box; the card box detection part is arranged on the card box bracket and is suitable for detecting the installation position of the card box; and the control system is suitable for controlling the card box bracket according to the acquired card box installation instruction and the signal of the card box detection part, installing the card box, and adjusting the relative positions of the card box integration part and the fluorescence quantitative detection part according to the acquired quantitative detection instruction so as to detect the concentration of the experimental sample. The gene sequencing library preparation device provided by the embodiment of the application can improve the integration level of the gene sequencing preparation device and reduce the operation difficulty.

Description

Gene sequencing library preparation facilities

Technical Field

The embodiment of the utility model provides a relate to biotechnology field, especially relate to a gene sequencing library preparation facilities.

Background

In recent years, the value of genomics data in clinical diagnosis and treatment is continuously discovered, wherein gene sequencing is an important technology for analyzing genomics data, and the value of genomics data is increasingly expanded and deepened in clinical application.

The gene sequencing experiment system is complex, and relates to multiple links of extraction, library establishment, sequencing and analysis, wherein the link of library establishment (namely the link of preparing a gene sequencing library) is one of the most critical factors for determining whether the whole gene sequencing process can realize automatic detection.

At present, gene sequencing library preparation mainly comprises a manual experiment method and a pipeline method, wherein a plurality of devices are required to be used in the two methods, the problem of cross contamination can be caused in the operation process, a large amount of manual operations are required to be added in the manual experiment method, and the methods have high requirements on the operation environment and space and are inconvenient to operate in order to reduce the cross contamination.

Therefore, how to reduce the operation difficulty of the gene sequencing library preparation device becomes a technical problem which needs to be solved urgently.

SUMMERY OF THE UTILITY MODEL

The embodiment of the utility model provides a technical problem who solves is how to reduce the operation degree of difficulty of gene sequencing library preparation facilities.

In order to solve the above problem, an embodiment of the present invention provides a gene sequencing library preparation apparatus, including:

the card box integration piece comprises a card box bracket and a card box, wherein the card box bracket is provided with a card box installation groove, and the card box is suitable for being installed in the card box installation groove;

the fluorescence module comprises a fluorescence quantitative detection part which can move relative to the card box integration part and is suitable for detecting the concentration of the experimental sample in the card box;

the card box detection piece is arranged on the card box bracket and is suitable for detecting the installation position of the card box;

and the control system is suitable for controlling the card box bracket according to the acquired card box installation instruction and the signal of the card box detection part, installing the card box, and adjusting the relative positions of the card box integration part and the fluorescence quantitative detection part according to the acquired quantitative detection instruction so as to detect the concentration of the experimental sample.

Optionally, the apparatus for preparing a gene sequencing library further comprises:

the ultrasonic module comprises an ultrasonic breaking component which can move relative to the card box integrated component and is suitable for breaking the experiment sample in the card box to obtain a broken experiment sample;

the control system is further adapted to adjust the relative positions of the cassette integration part and the ultrasonic break-out part according to the acquired ultrasonic break-out instruction so as to break out the experimental sample in the cassette.

Optionally, the cartridge comprises a sample tube and a breaking tube, the ultrasound module is adapted to break an experimental sample in the cartridge, and obtaining a broken experimental sample comprises:

breaking the experimental sample in the breaking pipe to obtain a broken experimental sample;

the control system is further adapted to adjust the relative positions of the cartridge integration part and the ultrasonic break-off part according to the acquired ultrasonic break-off instruction, so as to break off the experimental sample in the cartridge, and comprises:

according to the obtained ultrasonic breaking instruction, the experimental sample in the sample tube is moved into the breaking tube, and the relative positions of the card box integration piece and the ultrasonic breaking part are adjusted to break the experimental sample in the breaking tube.

Optionally, the cartridge further comprises a fluorescence quantitative tube, and the fluorescence module adapted to perform concentration detection on the experimental sample in the cartridge comprises:

detecting the concentration of the experimental sample in the fluorescent quantitative tube;

the control system is adapted to adjust the relative positions of the cartridge integration member and the fluorescence quantitative detection member according to the obtained quantitative detection instruction, so as to detect the concentration of the experimental sample, and comprises:

according to the obtained quantitative detection instruction, the experimental sample is put into the fluorescence quantitative tube, and the relative positions of the card box integration piece and the fluorescence quantitative detection part are adjusted to detect the concentration of the experimental sample.

a rotating electrical machine including a motor output shaft;

the main rotating disc is connected with the motor output shaft and is provided with a mounting groove for mounting the card box integrated piece;

the control system is suitable for controlling the card box bracket according to the acquired card box installation instruction and the signal of the card box detection part, installing the card box, and adjusting the relative positions of the card box integration part and the fluorescence quantitative detection part according to the acquired quantitative detection instruction so as to detect the concentration of the experimental sample, and comprises:

controlling the rotating motor to drive a card box bracket arranged on the main rotary disc to rotate to a card box loading and unloading position according to the acquired card box installation instruction, and controlling the card box bracket to install the card box according to a signal of the card box detection piece; according to the obtained quantitative detection instruction, the rotating motor is controlled to drive the card box integrated piece arranged on the main rotating disc to rotate to a quantitative detection position, and the relative positions of the card box integrated piece and the fluorescence quantitative detection part are adjusted to detect the concentration of the experimental sample.

the main rotating shaft is connected with the output shaft of the motor;

the main turntable includes:

the first main rotating disc is sleeved on the main rotating shaft;

and the second main rotary disc is sleeved on the main rotary shaft and is arranged at intervals with the first main rotary disc along the axial direction of the main rotary shaft.

Optionally, the cartridge comprises a closed cartridge.

Optionally, the cartridge integration comprises:

the card box loading and unloading module is arranged on the wall of the card box installation groove and is suitable for moving the card box arranged in the notch of the card box installation groove to the inside of the card box installation groove or moving the card box arranged in the card box installation groove to the notch of the card box installation groove;

and the card box locking module is fixed on the wall of the card box mounting groove and is suitable for locking the card box positioned in the card box mounting groove.

Optionally, the cartridge further comprises a sample application tip, and the cartridge assembly further comprises:

the suction head driving module is connected with the sample adding suction head and is suitable for driving the sample adding suction head to move to the sample tube of the card box;

and the liquid transferring pump device is fixed at the top of the card box mounting groove, is communicated with the sample adding suction head, and is suitable for being matched with the sample adding suction head to move the experimental sample in the sample tube.

Optionally, the apparatus for preparing a gene sequencing library of any one of the preceding embodiments, the cartridge further comprising a thermal cap for controlling the temperature of a sample tube in the cartridge, the cartridge assembly further comprising:

and the hot cover driving module is suitable for driving the hot cover to change the position or the state.

Optionally, the thermal cover driving module comprises:

a vertical driving means mounted to the cartridge integration member and adapted to drive the thermal cover to change a position or a state in a direction perpendicular to a sidewall of the cartridge;

and a horizontal driving means fixed to the vertical driving means and adapted to drive a position or a state of the thermal cover in a direction parallel to the side wall of the cartridge.

Optionally, the apparatus for preparing a gene sequencing library according to any one of the preceding embodiments, the cassette assembly further comprises:

the heating module, with the adjacent setting in card box mounting groove, and be fixed in the card box support is suitable for right the experiment sample in the card box goes up and down the temperature.

Optionally, the apparatus for preparing a gene sequencing library according to any one of the preceding embodiments, further comprising:

the shell is provided with a loading and unloading card box opening, and the loading and unloading card box opening is suitable for being correspondingly communicated with the notch of the card box mounting groove.

Optionally, the apparatus for preparing a gene sequencing library of any one of the preceding embodiments, the fluorescence module comprising:

the first direction quantitative driving part is suitable for driving the fluorescence quantitative detection part to move along the first direction;

the second direction quantitative driving part is suitable for driving the fluorescence quantitative detection part to move along a second direction, and the second direction is perpendicular to the first direction;

and the third direction quantitative driving part is suitable for driving the fluorescence quantitative detection part to move along a third direction, and the third direction is perpendicular to the first direction and the second direction.

Optionally, the apparatus of any preceding embodiment, the ultrasound module comprising:

a fourth direction breaking drive adapted to drive the ultrasonic breaking member to move in a fourth direction;

a fifth direction breaking drive adapted to drive the ultrasonic breaking member in a fifth direction, the fifth direction being perpendicular to the fourth direction;

a sixth direction breaking drive adapted to drive the ultrasonic breaking member in a sixth direction, the sixth direction being perpendicular to the fourth direction and perpendicular to the fifth direction.

Compared with the prior art, the embodiment of the utility model provides a technical scheme has following advantage:

the embodiment of the utility model provides a gene sequencing library preparation device, including card box integrated piece, supersound module, fluorescence module, card box detection piece and control system, card box integrated piece includes card box support and card box, and the card box support is seted up and is suitable for the card box mounting groove of installation card box; the fluorescence module comprises a fluorescence quantitative detection part which can move relative to the cartridge integration part and is suitable for detecting the concentration of the experimental sample in the cartridge; the card box detection piece is arranged on the card box bracket and is suitable for detecting the installation position of the card box; and the control system is suitable for controlling the card box bracket according to the acquired card box installation instruction and the signal of the card box detection part, installing the card box, and adjusting the relative positions of the card box integration part and the fluorescence quantitative detection part according to the acquired quantitative detection instruction to detect the concentration of the experimental sample.

It can be seen that the device for preparing a gene sequencing library provided in the embodiment of the present application integrates a card box capable of realizing preparation of an experimental sample, extraction of nucleic acid, construction and capture of a library, a card box integrated member capable of installing the card box, a fluorescence module capable of performing quantitative fluorescence detection, a card box detection member capable of obtaining the position of the card box, and a control system capable of automatically controlling each functional member, so that the preparation processes of the sample preparation of the gene sequencing library, extraction of nucleic acid, construction of the library, capture, quantitative fluorescence and the like can be integrally realized.

In the alternative, the gene sequencing library preparation device provided by the embodiment of the utility model also comprises a rotary motor, which comprises a motor output shaft; the main rotating disc is connected with the motor output shaft and provided with mounting grooves for mounting the card box integrated piece, and the number of the mounting grooves in the circumferential direction of the main rotating disc comprises at least two; the control system is suitable for controlling the rotating motor to drive the card box bracket arranged on the main rotary disc to rotate to a card box loading and unloading position according to the acquired card box installation instruction, and controlling the card box bracket to install the card box according to the signal of the card box detection piece; according to the obtained quantitative detection instruction, the rotating motor is controlled to drive the card box integrated piece arranged on the main rotating disc to rotate to a quantitative detection position, the relative position of the card box integrated piece and the fluorescence quantitative detection part is adjusted, and the concentration of the experimental sample is detected. It is thus clear that through being provided with and installing a plurality ofly the main carousel of card box integrated piece can drive through the rotation of main carousel the card box integrated piece accomplishes the step of breaking and fluorescence quantitative determination, can realize the preparation of many batches of gene sequencing samples simultaneously, improves the efficiency of obtaining the required sample of gene sequencing.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, it is obvious that the drawings in the following description are only embodiments of the present application, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

FIG. 1 is a perspective view of an apparatus for preparing a gene sequencing library provided in the examples of the present application;

FIG. 2 is a perspective view showing the internal structure of the apparatus for preparing a gene sequencing library shown in FIG. 1;

FIG. 3 is a perspective view showing the internal overall structure of the apparatus for preparing a gene sequencing library shown in FIG. 1;

FIG. 4 is a perspective view showing a partial structure of the apparatus for preparing a gene sequencing library shown in FIG. 3;

FIG. 5 is a schematic view showing the structure of a cartridge assembly of the gene sequencing library preparation apparatus shown in FIG. 3;

FIG. 6 is a schematic diagram of the operation state of the apparatus for preparing a gene sequencing library provided in the embodiment of the present application, which performs ultrasonic disruption;

FIG. 7 is an enlarged view of a part A of the structure of an ultrasonic breaking part of the apparatus for preparing a gene sequencing library shown in FIG. 6;

FIG. 8 is a schematic view showing the operation of the apparatus for preparing a gene sequencing library for fluorescence quantitative detection according to the embodiment of the present application;

FIG. 9 is an enlarged schematic view of a part of the fluorescent quantitative section B of the apparatus for preparing a gene sequencing library shown in FIG. 8.

/>

Detailed Description

As known from the background art, the operation difficulty of the gene sequencing library preparation device is high.

For the operation degree of difficulty that reduces gene sequencing library preparation facilities, the embodiment of the utility model provides a gene sequencing library preparation facilities includes:

the card box integration piece comprises a card box bracket and a card box, wherein the card box bracket is provided with a card box installation groove, and the card box is suitable for being installed in the card box installation groove; the fluorescence module comprises a fluorescence quantitative detection part which can move relative to the card box integration part and is suitable for detecting the concentration of the experimental sample in the card box;

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.

It should be noted that the indication of the direction or the positional relationship referred to in the present specification is based on the direction or the positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and it is not intended to indicate or imply that the indicated device must have a specific direction, be configured in a specific direction, and thus, should not be construed as limiting the present invention.

Referring to FIGS. 1 to 5, FIG. 1 is a perspective view of a gene sequencing library preparation apparatus according to an embodiment of the present invention, FIG. 2 is a perspective view of an internal partial structure of the gene sequencing library preparation apparatus shown in FIG. 1, FIG. 3 is a perspective view of an internal overall structure of the gene sequencing library preparation apparatus shown in FIG. 1, FIG. 4 is a perspective view of a partial structure of the gene sequencing library preparation apparatus shown in FIG. 3, and FIG. 5 is a schematic structural view of a cartridge assembly of the gene sequencing library preparation apparatus shown in FIG. 3.

As shown in the figure, the gene sequencing library preparation apparatus provided in the embodiments of the present application includes:

the card box integration member 1 comprises a card box bracket 11 and a card box 12, wherein the card box bracket 11 is provided with a card box installation groove 111, and the card box 12 is suitable for being installed in the card box installation groove 111;

a fluorescence module 3, including a fluorescence quantitative detection part 31 movable relative to the cartridge assembly 1, adapted to perform concentration detection on the test sample in the cartridge 12;

a cartridge detecting member 4 mounted to the cartridge holder 11 and adapted to detect a mounting position of the cartridge 12;

and the control system is suitable for controlling the card box bracket 11 and installing the card box 12 according to the acquired card box installation instruction and the signal of the card box detection part 4, and adjusting the relative positions of the card box integration part 1 and the fluorescence quantitative detection part 31 according to the acquired quantitative detection instruction so as to detect the concentration of the experimental sample.

In this embodiment, the test sample in the cartridge may be a test sample capable of directly performing concentration detection (a prepared test sample having completed sample preparation), and in some embodiments, may also be a test sample capable of performing concentration detection after being further processed by the gene sequencing library preparation apparatus provided in this application.

The experimental sample referred to in the "to detect the concentration of the experimental sample" refers to the experimental sample obtained in the whole experimental process (nucleic acid extraction, library construction and capture) of gene sequencing library preparation, and may include: extracting an experimental sample, constructing the experimental sample and capturing the experimental sample.

The cartridge holder 11 of the cartridge integrated unit 1 can install the cartridge 12 and various functional parts related to the process of the cartridge 12, improve the assembly integration of the cartridge 12, and facilitate the control of the cartridge 12 and the process of an experimental sample.

In some embodiments, the cartridge 12 may comprise a closed cartridge, so that it is ensured that cross contamination of the sample (each test sample and the interrupted test sample) does not occur when the test sample inside the cartridge 12 is processed on the basis of the integrated loading and unloading of the cartridge 12, and the requirement of the genetic sequencing library preparation apparatus on the experimental environment can be reduced. The fluorescence module 3, including for the fluorescence quantitative determination part 31 that the integrated piece 1 of card box removed, adjust the position relation between fluorescence quantitative determination part 31 and the integrated piece 1 of card box in real time through control system, realize the concentration detection of experimental sample fast, easy operation, the control of being convenient for. The card box detection member 4 can be arranged on the wall of the card box installation groove 111 of the card box bracket 11, detect the position information of the card box 12 in real time, transmit the detected information to the control system, and control the card box integration member 1 to complete the installation and the unloading of the card box 12 through the control system.

The "quantitative fluorescence detecting means 31 movable with respect to the cartridge assembly 1" means that the fluorescence module 3 and the cartridge assembly 1 are movable with respect to each other, that is, the position of the fluorescence module 3 is fixed and the cartridge assembly 1 is movable; or the position of the card box integration member 1 is fixedly arranged, and the position of the fluorescent module 3 can be movably arranged; or both may be movably positioned.

When the fluorescent card box integrated piece 1 works, when the position of the fluorescent module 3 is movably arranged and the position of the card box integrated piece 1 is fixedly arranged, the fluorescent quantitative detection part 31 can be controlled to move through the control system according to the quantitative detection instruction until the fluorescent quantitative detection part 31 reaches the position corresponding to the card box integrated piece 1, and the movement is stopped, so that the concentration detection of the experiment sample in the card box 12 in the card box integrated piece 1 is realized.

When the position of the card box integration member 1 is movably arranged and the position of the fluorescence quantitative detection component 31 is fixedly arranged, the control system can control the card box integration member 1 to move according to a quantitative detection instruction until the card box integration member 1 reaches the position corresponding to the fluorescence quantitative detection component 31, and the movement is stopped, so that the concentration detection of the experiment sample in the card box 12 in the card box integration member 1 is realized.

When the positions of the card box integrated piece 1 and the fluorescent quantitative detection part 31 are both movably arranged, the fluorescent quantitative detection part 31 and the card box integrated piece 1 can be controlled to move by the control system according to a quantitative detection instruction until the two parts reach the same position, and the concentration detection of an experimental sample is realized.

Like this, when carrying out gene sequencing library preparation through the gene sequencing library preparation facilities that this application embodiment provided, can obtain the signal that card box detection piece 4 through control system according to the initial instruction (card box installation instruction) that the library that receives prepared, control card box integrated piece 1 will be equipped with the card box 12 of experimental sample and install in card box mounting groove 111, then according to each flow instruction of gene sequencing library preparation, when needs carry out the quality testing to the experimental sample, control system obtains the quantitative detection instruction, adjust the position of card box integrated piece 1 and fluorescence module 3, realize the concentration detection of experimental sample.

Of course, the respective instructions may be acquired by the control panel 51 of the control system according to the specific operation steps.

It can be seen that the gene sequencing library preparation device provided in the embodiment of the present application integrates the card box 12 capable of implementing preparation of an experimental sample, extraction of nucleic acid, construction and capture of a library, the card box integration component 1 capable of installing the card box 12, the ultrasonic module 2 capable of performing ultrasonic interruption, the fluorescent module 3 capable of performing fluorescent quantitative detection, the card box detection component 4 capable of obtaining the position of the card box 12, and the control system capable of automatically controlling each functional component, so that the preparation processes of sample preparation, extraction of nucleic acid, construction of a library, capture, fluorescent quantitative detection and the like of a gene sequencing library can be integrally implemented, and when the sample concentration detection is required, the concentration detection of the experimental sample can be immediately implemented by adjusting the relative positions of the card box integration component 1 and the fluorescent module 3 through the control system, the integration degree of the gene sequencing library preparation device can be improved, and the operation difficulty of the gene sequencing library preparation device can be reduced.

In order to enrich the types of experimental samples that can be processed by the gene sequencing library preparation device and improve the practicability of the gene sequencing library preparation device, in one embodiment, the gene sequencing library preparation device provided in the embodiments of the present application may further include:

the ultrasonic module 2 comprises an ultrasonic breaking component 21 which can move relative to the card box integration component 1 and is suitable for breaking the experiment sample in the card box 12 to obtain a broken experiment sample;

the control system is further adapted to adjust the relative positions of the cartridge integrated unit 1 and the ultrasound interruption unit 21 in response to the acquired ultrasound interruption instructions to interrupt the test sample in the cartridge 12.

Of course, the above-mentioned "ultrasonic breaking component 21 movable relative to the cartridge integrated component 1" means that the ultrasonic module 2 and the cartridge integrated component 1 are movable relative to each other, that is, the position of the ultrasonic module 2 is fixed, and the cartridge integrated component 1 is movable; or the position of the card box integration piece 1 is fixedly arranged, and the position of the ultrasonic module 2 can be movably arranged; or both may be movably positioned.

At specific during operation, when the position of supersound module 2 is portable setting, the position of card box integrated package 1 is when fixed setting, can break the removal that part 21 was broken to the instruction control supersound through control system according to the supersound, stops moving when the supersound breaks part 21 and arrives the position corresponding with the card box integrated package 1, realizes that the supersound of the inside experiment sample of card box 12 in the card box integrated package 1 breaks.

When the position of the cartridge integrated component 1 is movably arranged and the position of the ultrasonic interruption component 21 is fixedly arranged, the cartridge integrated component 1 can be fixed on the ultrasonic interruption fixing seat 22 (shown in fig. 3), and the control system can control the cartridge integrated component 1 to move according to an ultrasonic interruption instruction until the cartridge integrated component 1 stops moving when reaching the same corresponding position as the ultrasonic interruption component 21 or the fluorescence quantitative detection component 31, so that the concentration detection of the interrupted experiment sample in the cartridge 12 in the cartridge integrated component 1 is realized.

When the positions of the card box integrated part 1 and the ultrasonic breaking part 21 are movably arranged, the ultrasonic breaking part 21 and the card box integrated part 1 can be controlled to move through the control system according to an ultrasonic breaking instruction until the two parts reach the same position, and the ultrasonic breaking of the experimental sample is realized.

Supersound module 2, including can for the part 21 is broken to the supersound that the integrated piece 1 of card box removed, through the real-time adjustment supersound of control system the position relation between part 21 and the integrated piece 1 of card box, realize the operation of breaking of experiment sample fast, easy operation, the control of being convenient for.

Therefore, the practicability of the gene sequencing library preparation device can be improved, the combination mode of the functional modules (the ultrasonic module 2 and the fluorescent module 3) can be flexibly adjusted, and the preparation of different types of experimental samples can be realized.

Of course, the cartridge assembly 1 of the gene sequencing library preparation apparatus provided in the embodiments of the present application may further include a plurality of structures in order to improve convenience and efficiency in processing of an experimental sample.

To facilitate loading, unloading, and control of the cartridge 12, in one embodiment, please continue to refer to fig. 3 and 5.

As shown in the figure, the cartridge assembly 1 of the gene sequencing library preparation apparatus provided in the embodiments of the present application may further include: a cartridge loading and unloading module 112 disposed on a wall of the cartridge mounting slot 111 and adapted to move the cartridge 12 mounted in the slot of the cartridge mounting slot 111 into the cartridge mounting slot 111 or to move the cartridge 12 mounted in the cartridge mounting slot 111 into the slot of the cartridge mounting slot 111;

a cartridge locking module 113 fixed to a wall of the cartridge installation slot 111 and adapted to lock the cartridge 12 positioned inside the cartridge installation slot 111.

Thus, the cartridge loading and unloading module 112 capable of loading and unloading the cartridge 12 and the cartridge locking module 113 capable of fixing the cartridge 12 are integrated on the cartridge holder 11, and the loading and unloading of the cartridge 12 is automatically controlled by the control system, which is simple in operation.

Of course, in other embodiments, the cartridge 12 may be manually installed in the cartridge installation slot 111 and locked by the locking device when the cartridge 12 is installed.

To facilitate processing of the experimental sample inside the cartridge 12, in one embodiment, the cartridge holder 11 may also be designed according to the specific structure of the cartridge 12, please continue to refer to fig. 3 and 5.

As shown in the figure, the cartridge 12 of the gene sequencing library preparation apparatus provided in the embodiment of the present application may further include a sample application tip, and the cartridge assembly 1 may further include:

a tip driving module 114 connected to the sample-adding tip and adapted to drive the sample-adding tip to move to the sample tube of the cartridge 12; and a liquid transfer pump device 115 fixed on the top of the cartridge mounting groove 111, communicating with the sample-adding suction head, and adapted to cooperate with the sample-adding suction head to move the experimental sample in the sample tube.

It will be readily appreciated that when the cartridge 12 is provided with a interrupt tube 121 or a quantitative fluorescent tube 122 to satisfy different functional requirements, the cartridge assembly 1 comprises:

a suction head driving module 114 connected to the sample-adding suction head and adapted to drive the sample-adding suction head to move to the breaking tube 121 or the fluorescence quantitative tube 122 of the cartridge 12;

and a liquid transfer pump device 115 fixed to the top of the cartridge mounting groove 111, communicating with the sample addition tip, and adapted to cooperate with the sample addition tip to move the test sample in the break tube 121 or the fluorescence quantitative tube 122.

Like this, can avoid the experimental sample in the card box 12 the condition that takes place cross contamination in concrete operation, reduce gene sequencing library preparation facilities to experimental environment's requirement to can be according to actual need, the nimble test tube quantity that sets up in the card box 12 and the test tube function that corresponds.

In order to prevent the evaporation of water in the sample inside the cartridge 12 and the condensation of water on the tube cap of the sample tube containing the sample during the preparation of the gene sequencing library, in one embodiment, the apparatus for preparing the gene sequencing library provided in the embodiment of the present application is further provided with a device capable of controlling the temperature of the sample tube in the cartridge 12, please refer to fig. 1-5.

As shown in the figure, the cartridge 12 further includes a thermal cover that controls the temperature of the sample tube in the cartridge 12, and the cartridge assembly 1 further includes:

a thermal cover driving module 116 adapted to drive the thermal cover to change position or state.

The "change of state" refers to changing the opening and closing state of the thermal cover on the side walls of the cartridge 12 to achieve control and adjustment of the temperature inside the cartridge 12.

Like this, use hot lid drive module 116 to set up in hot lid on the lateral wall of card box 12 carries out temperature control, satisfy the needs in the gene sequencing library preparation process, and simultaneously, in the mineral oil sealing mode that current method commonly used, because mineral oil's operating characteristic, make the experimental sample temperature that is close to mineral oil at the intensification in-process high, the experimental sample that is close to mineral oil in the cooling process is low on the side, the temperature that leads to the experimental sample in the sample tube is inhomogeneous, and use the hot lid drive module 116 that this application embodiment provided to carry out temperature control's mode to the hot lid, can avoid using mineral oil to seal, thereby can improve the condition of being heated of experimental sample.

In order to drive the thermal cover and improve the flexibility of moving the thermal cover, in one embodiment, the thermal cover driving module 116 of the apparatus for preparing a gene sequencing library provided in the embodiments of the present application includes:

a vertical driving means 1161 installed at the cartridge integration member 1, adapted to drive the thermal cover to change a position or a state in a direction perpendicular to a sidewall of the cartridge 12;

a horizontal driving means 1162 fixed to the vertical driving means 1161 and adapted to drive a position or state of the thermal cover in a direction parallel to the sidewall of the cartridge 12.

The "direction perpendicular to the side wall of the cartridge 12" is the direction indicated by the arrow C shown in fig. 4, the "direction parallel to the side wall of the cartridge 12" is the direction indicated by the arrow D shown in fig. 4, and the directions indicated by the arrows C and D are perpendicular to each other.

Of course, "vertical" and "horizontal" as used herein mean that the driving directions of the two-directional driving means of the gene sequencing library preparation apparatus are perpendicular to each other, and are not limited to vertical and horizontal.

When the thermal cover needs to be controlled to close the cartridge 12, the horizontal driving device 1162 controls the thermal cover to move in the vertical direction, so as to control the thermal cover to turn and push from the initial position (which is in an open state) to the preset position; when the hot lid is turned over to a predetermined position, and the lid is vertically aligned with the sample tube, the vertical driving device 1161 is controlled to move downward, so as to push and press the hot lid to close the sample tube, and the hot lid is kept at the closed position. When the heating process is finished, the vertical driving device 1161 moves reversely to perform vertical upward displacement according to the control instruction, the contact between the thermal cover and the thermal cover is broken, and the thermal cover is far away from the sample tube; the horizontal drive 1162 moves in reverse away from the hot lid so that the hot lid can return to the position in which the hot lid is open.

Thus, the temperature inside the cassette 12 can be flexibly controlled, and the operational requirements for gene sequencing library preparation can be met.

The nucleic acid extraction is the most basic operation step in performing a molecular experiment, and is the basic operation step that a gene sequencing library preparation apparatus can smoothly perform, so that in order to realize the ultrasound interruption of the gene sequencing library preparation apparatus on an experimental sample or the concentration detection of the experimental sample, in a specific embodiment, the gene sequencing library preparation apparatus provided in the embodiment of the present application may further add components required for nucleic acid extraction, and specifically, please refer to fig. 4 continuously.

As shown in the figure, the cassette assembly 1 of the gene sequencing library preparation apparatus provided in the embodiments of the present application may further include:

and the heating module 117 is arranged adjacent to the cartridge mounting groove 111, fixed to the cartridge support 11, and adapted to heat the test sample in the cartridge 12.

The temperature inside the card box 12 is controlled by the heating module 117, so that the requirement of the experimental sample on the temperature in the preparation process of the gene sequencing library is met.

In order to realize the accurate positioning of the fluorescence module 3 and the card box integrated member 1, in one embodiment, the fluorescence quantitative detection component 31 of the fluorescence module 3 is configured as a multi-degree-of-freedom structure, specifically, the fluorescence module 3 further includes:

a first direction quantitative driving member adapted to drive the fluorescence quantitative detection part 31 to move in a first direction;

a second direction quantitative driving member adapted to drive the fluorescent quantitative detection member 31 to move along a second direction, the second direction being perpendicular to the first direction;

and a third direction quantitative driving member adapted to drive the fluorescent quantitative detection member 31 to move along a third direction, wherein the third direction is perpendicular to the first direction and perpendicular to the second direction.

For convenience of understanding, the first direction, the second direction and the third direction may be understood as an x direction, a y direction and a z direction, respectively, and when the first direction is the x direction, the second direction and the third direction may be the y direction and the z direction, respectively, and when the first direction is the y direction, the second direction and the third direction may be the x direction and the z direction, respectively.

In this way, the fluorescent module 3 and the fluorescent quantitative detection component 31 are connected by the quantitative driving member with multiple degrees of freedom, after the positions of the fluorescent module 3 and the card box integrated component 1 are aligned, the fluorescent quantitative detection component 31 is further finely adjusted by the quantitative driving member with multiple degrees of freedom, so that the accurate positioning of the card box 12 in the fluorescent quantitative detection component 31 and the card box integrated component 1 is realized, and the accuracy of concentration detection can be improved.

On the other hand, in order to achieve precise positioning of the ultrasound module 2 and the cartridge integrated component 1, in an embodiment, the ultrasound breaking component 21 of the ultrasound module 2 is configured as a structure with multiple degrees of freedom, and in particular, please continue to refer to fig. 2.

As shown in the figure, the ultrasound module 2 of the gene sequencing library preparation apparatus provided in the embodiment of the present application may further include:

a fourth direction breaking drive 231 adapted to drive said ultrasonic breaking member 21 in a fourth direction;

a fifth direction breaking drive 232 adapted to drive said ultrasonic breaking member 21 in a fifth direction, said fifth direction being perpendicular to said fourth direction;

a sixth direction breaking drive 233 adapted to drive said ultrasonic breaking member 21 in a sixth direction, perpendicular to said fourth direction and perpendicular to said fifth direction.

Here, the fourth direction, the fifth direction and the sixth direction may be understood as x direction, y direction and z direction, respectively, and when the fourth direction is x direction, the fifth direction and the sixth direction may be y direction and z direction, respectively, and when the fourth direction is y direction, the fifth direction and the sixth direction may be x direction and z direction, respectively.

Like this, break the part with supersound module 2 and supersound and realize being connected through the driving piece that breaks that has multi freedom between 21, can be after the position with supersound module 2 and card box integrated package 1 is aimed at, break the driving piece through having multi freedom and carry out further fine setting to supersound breaking part 21, realize that supersound breaks the accurate location of the sample tube in the card box 12 in part 21 and the card box integrated package 1, can improve the precision that supersound breaks the result.

In order to facilitate the integrated configuration and assembly of the aforementioned functional components, in a specific embodiment, the apparatus for preparing a gene sequencing library provided in the embodiments of the present application may further include:

the shell 8 is provided with a loading and unloading card box opening 81, and the loading and unloading card box opening 81 is suitable for being correspondingly communicated with the notch of the card box installation groove 111.

It is easily understood that the removable cartridge slot 81 described herein is adapted to be in corresponding communication with the slot of the cartridge mounting slot 111, which means that when the genetic sequencing library preparation apparatus includes only one cartridge mounting slot 111, the removable cartridge slot 81 can be in corresponding communication with the slot of the cartridge mounting slot 111 at all times, enabling the cartridge mounting; when the gene sequencing library preparation apparatus includes a plurality of cartridge mounting grooves 111, the loading and unloading cartridge slot 81 can be in corresponding communication with the slot opening of the cartridge mounting groove 111 by adjusting the position of the cartridge mounting groove 111.

It can be seen that the apparatus for preparing a gene sequencing library provided in the embodiment of the present application may initially place the cartridge 12 containing all the experimental samples to be prepared through the loading and unloading cartridge slot 81, or first use a part of the cartridge assembly 1 to install a small number of cartridges 12 to complete the preparation of the gene sequencing library of the part of the small number of cartridges 12, when there is an additional experimental sample cartridge 12 to be processed, then select the cartridge assembly 1 not loaded into the cartridge 12 through the control panel 51 of the control system, and send a command to the control component 52 of the control system, adjust the position of the cartridge assembly 1, so that the slot of the cartridge installation slot 111 can be aligned and communicated with the loading and unloading cartridge slot 81, install a new cartridge 12 to be processed to achieve the breaking of the experimental sample or the concentration detection of the experimental sample, or withdraw the cartridge 12 having completed the preparation of the gene sequencing library and then load the new cartridge 12, thereby achieving the flexible control of the apparatus for preparing the gene sequencing library, and the operation is simple.

Specifically, with continuing reference to fig. 1 and 2, as shown in the figure, in order to facilitate the overall packaging of the gene sequencing library preparation apparatus, in an embodiment, each integrated functional module is integrally packaged by a housing 8, as shown in the figure, the ultrasound module 2, the fluorescence module 3, and the control component 52 and the control sensor 53 of the control system may be fixed on a base of the housing 8, and after each functional module is integrated on the base of the housing 8, in order to facilitate the control of the operation of the gene sequencing library preparation apparatus, in an embodiment, a control panel 51 may be disposed on the housing 8, and according to the overall structural size of the gene sequencing library apparatus, in order to facilitate the control of the control panel 51, the control panel 51 may be disposed on a side wall of the housing 8, or may be disposed on the top of the housing 8, which is convenient for the control.

Of course, the control mode of the control panel 51 may be a touch type or a semi-touch type, which only needs to meet the actual requirement.

The respective instructions acquired by the control system can be acquired through the control panel 51.

It should be noted that the control sensor 53 can be used to determine the angle formed by the ultrasonic module 2 and the initial position of the cartridge assembly 1 and determine the angle formed by the fluorescence module 3 and the initial position of the cartridge assembly 1, so that when the operation of the genetic sequencing library preparation apparatus is controlled by the control panel 51, the control unit 52 can adjust the positions of the ultrasonic breaking unit 21 of the ultrasonic module 2 and the cartridge assembly 1 according to the angle information transmitted by the control sensor 53, so as to break the test sample, and the control unit 52 can adjust the relative positions of the fluorescence quantitative detection unit 31 of the fluorescence module 3 and the cartridge assembly 1, so as to detect the concentration of the test sample.

The housing 8 is provided with a loading and unloading cassette port 81 which can facilitate the installation and unloading of the cassette 12 and realize the preparation of the gene sequencing library.

According to gene sequencing library preparation facilities's overall structure setting, can realize once only accomplishing the preparation of gene sequencing sample in the same test tube in one card box 12, at this moment, can directly carry out the operation that the sample breaks and the concentration detection of experimental sample to this test tube to accomplish the preparation of the experimental sample in this test tube. Of course, the tubes in the cartridge 12 may be designed for zonal sorting in order to avoid cross-contamination between samples obtained from different processing steps.

Therefore, in some embodiments, the test tubes inside the cartridge 12 can be functionally arranged, specifically, please refer to fig. 6 and 7, fig. 6 is a schematic diagram of the operation state of the gene sequencing library preparation apparatus provided in the embodiments of the present application for performing ultrasonic disruption, and fig. 7 is an enlarged view of the structure of the part a of the ultrasonic disruption part of the gene sequencing library preparation apparatus shown in fig. 6.

As shown in the figure, in order to facilitate different operations on the test samples in the respective test tubes in the cartridge 12, meet the requirements of the test tubes for different operations, and reduce the design and use cost of the test tubes, the cartridge 12 may include a sample tube and a breaking tube 121, the ultrasound module 2 is adapted to break the test sample in the cartridge 12, and obtaining the broken test sample includes:

breaking the experimental sample in the breaking pipe 121 to obtain a broken experimental sample;

the control system, adapted to adjust the relative positions of the cartridge integrated member 1 and the ultrasound disrupting element 21 according to the acquired ultrasound disrupting instructions to disrupt the experimental sample in the cartridge 12, comprises:

according to the acquired ultrasonic interruption instruction, the experimental sample in the sample tube is moved into the interruption tube 121, and the relative positions of the cassette integration member 1 and the ultrasonic interruption member 21 are adjusted to interrupt the experimental sample in the interruption tube 121.

It should be noted that the "experimental sample" may be an experimental sample after being subjected to the operation of sample preparation and nucleic acid extraction.

Therefore, the test tubes in the card box 12 are functionally distinguished, so that experimental samples with different properties can be conveniently processed, the processing requirements of the experimental samples are met, and the practicability of the gene sequencing library preparation device is improved.

For the experiment sample needing ultrasonic breaking, because the breaking tube 121 is arranged, during the concrete treatment, the control system can be utilized to firstly realize the treatment of the experiment sample in the sample tube (the sample preparation and the nucleic acid extraction of the experiment sample), then the treated experiment sample (namely the extraction experiment sample conforming to the extraction standard concentration value in the sample nucleic acid extraction process) is transferred into the breaking tube 121, then the relative positions of the ultrasonic breaking component 21 of the ultrasonic module 2 and the card box integrated component 1 are adjusted, the ultrasonic breaking component 21 of the ultrasonic module 2 is moved to the position of the breaking tube 121, the ultrasonic breaking of the experiment sample in the breaking tube 121 is completed, and then the library construction operation is carried out on the breaking experiment sample conforming to the size of the standard breaking sample, the sample obtained by library construction needs nucleic acid quantification, at this time, the positions of the fluorescence module 3 and the card box integration member 1 can be adjusted through the control system, so that the fluorescence quantitative detection part 31 of the fluorescence module 3 performs quality detection on the experimental sample (library construction sample) obtained by library construction, after the quality detection of the library construction sample is completed, the next capturing operation is performed on the library construction sample meeting the requirements, the fragment analysis is performed on the experimental sample obtained by capturing treatment, and nucleic acid quantification is performed, at this time, the fluorescence quantitative detection is continuously performed on the captured sample in the broken tube 121 through the fluorescence quantitative detection part 31, and finally the experimental sample meeting the gene library sequencing requirement is obtained. Therefore, the required test tube environment can be provided for the experimental sample needing ultrasonic treatment, so that the operation of the gene sequencing library preparation device is more convenient and flexible.

For the experimental sample which does not need to be subjected to ultrasonic interruption, the operations of sample preparation, sample nucleic acid extraction, library construction and capture of the experimental sample can be completed in a sample tube, so that the gene sequencing library preparation device is simple to operate.

Of course, in other embodiments, the test tubes in the cartridge 12 may be further arranged in a sorting manner in order to improve the operational rationality of the gene sequencing library preparation and to meet the experimental environment required for each process of the experimental sample. Specifically, please refer to fig. 8 and 9, wherein fig. 8 is a schematic diagram of an operation state of the gene sequencing library preparation apparatus for performing fluorescent quantitative detection according to the embodiment of the present application, and fig. 9 is an enlarged schematic diagram of a part B of the fluorescent quantitative part of the gene sequencing library preparation apparatus shown in fig. 8.

As shown in the figure, the cartridge 12 may further include a fluorescence quantitative tube 122, and the fluorescence module 3 is adapted to perform concentration detection on the experimental sample in the cartridge 12 and includes:

performing concentration detection on the experimental sample in the fluorescence quantitative tube 122;

the control system, adapted to adjust the relative positions of the cartridge assembly 1 and the fluorescence quantitative determination unit 31 according to the obtained quantitative determination instruction, so as to detect the concentration of the test sample, comprises:

according to the obtained quantitative determination instruction, the experimental sample is put into the quantitative fluorescence detection tube 122, and the relative positions of the cartridge assembly 1 and the quantitative fluorescence detection part 31 of the gene sequencing library preparation apparatus are adjusted to detect the concentration of the experimental sample.

Like this, can deposit the experiment sample in advance in the sample pipe in card box 12, when the experiment sample of handling needs to carry out the ultrasonic interruption and handles, at first, with the experiment sample in the sample pipe (the experiment sample this moment is for the extraction experiment sample that accords with sample nucleic acid extraction in-process extraction standard concentration value) remove to in breaking the pipe 121, control system adjusts the position of ultrasonic interruption part 21, break the experiment sample supersound in the pipe 121 of breaking, and then carry out the aassessment of breaking the effect to breaking the experiment sample in the pipe 121 of breaking, obtain the experiment sample that breaks that accords with standard and breaks the sample size.

Then, the broken experimental sample is subjected to subsequent library construction processing to obtain a constructed experimental sample, the constructed experimental sample is moved to the fluorescent quantitative tube 122, and the concentration detection of the constructed experimental sample is realized by the fluorescent quantitative detection part 31 of the fluorescent module 3.

Finally, the constructed experiment sample meeting the constructed standard concentration value is moved to a sample tube for capture processing to obtain a captured experiment sample, the captured experiment sample is moved to a fluorescent quantitative tube 122, the concentration detection of the captured experiment sample is realized through a fluorescent quantitative detection part 31 of the fluorescent module 3, and finally the captured experiment sample meeting the captured standard concentration value is obtained, namely, the sequencing experiment sample meeting the gene sequencing library standard is finally obtained.

When the processed experimental sample does not need to be processed by the ultrasonic module 2, the original experimental sample in the sample tube is firstly subjected to sample preparation processing to obtain a prepared experimental sample, and certainly, the nucleic acid extraction processing can be directly performed on the experimental sample which does not need to be prepared.

Then, the prepared experimental sample is subjected to sample nucleic acid extraction treatment to obtain an extracted experimental sample, and the concentration of the extracted experimental sample is detected by the fluorescent quantitative detection part 31 of the fluorescent module 3.

Then, the extraction experiment sample meeting the extraction standard concentration value is moved to a sample tube to perform library construction processing, so as to obtain a constructed experiment sample, the constructed experiment sample is moved to a fluorescent quantitative tube 122, and the concentration detection of the constructed experiment sample is realized through a fluorescent quantitative detection part 31 of the fluorescent module 3.

Therefore, test tubes meeting experimental conditions can be correspondingly arranged according to different processes of gene sequencing library preparation, and the experimental cost can be reduced while the practicability of the gene sequencing library preparation device is improved.

In order to meet the requirement of preparing a larger number of gene sequencing libraries, a plurality of cassette integration units 1 can be provided to complete the preparation of gene sequencing libraries of a plurality of cassettes 12 at a time. Please refer to fig. 2-4.

As shown in the figure, the gene sequencing library preparation apparatus may further include:

a rotating electrical machine including a motor output shaft;

the main rotating disc 6 is connected with the motor output shaft and provided with mounting grooves for mounting the card box integrated part 1, and the number of the mounting grooves in the circumferential direction of the main rotating disc 6 comprises at least two;

the control system, adapted to control the cartridge holder 11, mount the cartridge 12, according to the acquired cartridge mounting instruction and the signal of the cartridge detecting member 4, adjust the relative positions of the cartridge integrator 1 and the ultrasonic disrupting member 21 according to the acquired ultrasonic disrupting instruction to disrupt the test sample in the cartridge 12, and adjust the relative positions of the cartridge integrator 1 and the quantitative fluorescence detecting member 31 according to the acquired quantitative detection instruction to detect the concentration of the test sample, includes:

controlling the rotating motor to drive a card box bracket 11 arranged on the main rotary disc 6 to rotate to a card box loading and unloading position according to the acquired card box installation instruction, controlling the card box bracket 11 according to the signal of the card box detection piece 4, and installing the card box 12;

controlling the rotating motor to drive the card box integration member 1 arranged on the main rotary disc 6 to rotate to an ultrasonic breaking position according to the acquired ultrasonic breaking instruction, and adjusting the relative positions of the card box integration member 1 and the ultrasonic breaking member 21 to break the experimental sample in the card box 12;

according to the obtained quantitative detection instruction, the rotating motor is controlled to drive the card box integration member 1 arranged on the main rotating disc 6 to rotate to a quantitative detection position, and the relative positions of the card box integration member 1 and the fluorescence quantitative detection part 31 are adjusted to detect the concentration of the experimental sample.

The "circumferential direction" is the R direction shown in fig. 4.

Of course, when the cartridge 12 includes a sample tube and a breaking tube 121, the step of "controlling the rotary motor to rotate the cartridge integrated member 1 mounted on the main turntable 6 to an ultrasonic breaking position according to the acquired ultrasonic breaking instruction, and adjusting the relative positions of the cartridge integrated member 1 and the ultrasonic breaking member 21 to break the experimental sample in the cartridge 12" may include:

according to the acquired ultrasonic breaking instruction, the experimental sample is moved to the breaking pipe 121 from the sample pipe, the rotating motor is controlled to drive the card box integration member 1 arranged on the main rotating disc 6 to rotate to the ultrasonic breaking position, the relative positions of the card box integration member 1 and the ultrasonic breaking part 21 are adjusted, and the experimental sample in the card box 12 is broken.

When the card box 12 further includes the fluorescence quantitative tube 122, the "controlling the rotating motor to drive the card box integrated part 1 installed on the main turntable 6 to rotate to the quantitative detection position according to the obtained quantitative detection instruction, adjusting the relative positions of the card box integrated part 1 and the fluorescence quantitative detection part 31, and detecting the concentration of the experimental sample" may include:

according to the obtained quantitative detection instruction, the experimental sample is put into the fluorescence quantitative tube 122, the rotating motor is controlled to drive the card box integration member 1 arranged on the main rotating disc 6 to rotate to the quantitative detection position, the relative position of the card box integration member 1 and the fluorescence quantitative detection part 31 is adjusted, and the concentration of the experimental sample is detected.

Thus, a plurality of mounting grooves are arranged on one main turntable 6, a plurality of card box integration members 1 can be mounted, so that the preparation of experimental samples in a plurality of card boxes 12 is realized simultaneously, and the preparation efficiency of a gene sequencing library is improved.

In some embodiments, 4 mounting slots may be provided on one

main turntable

6, and 4 cartridge modules 1 may be provided in the circumferential direction. Of course, in other embodiments, more installation slots may be provided, and the design may be specifically performed according to the actual size of the gene sequencing library preparation apparatus or the number of gene sequencing samples to be prepared.

In some embodiments, in order to prepare a larger number of gene sequencing libraries at a time, the design can also be performed in a direction perpendicular to the main rotating disk 6, please continue to refer to fig. 2-4.

the main rotating shaft 7 is connected with the output shaft of the motor;

the main turntable 6 includes:

the first main rotating disc 61 is sleeved on the main rotating shaft 7;

and the second main rotating disc 62 is sleeved on the main rotating shaft 7 and is arranged at an interval with the first main rotating disc 61 along the axial direction of the main rotating shaft 7.

Thus, a plurality of main rotating discs 6 (a first main rotating disc 61 and a second main rotating disc 62) can be arranged on the main rotating shaft 7, and a sufficient number of main rotating discs 6 can be arranged according to the actual size of the gene sequencing library preparation device, so as to meet the preparation requirements of a large number of gene sequencing libraries.

In order to be able to prepare large quantities of gene sequencing libraries, it is desirable to handle multiple cassettes 12, and thus, in one embodiment, the number of cassette ports 81 can be amplified, with continued reference to FIGS. 1-5.

As shown in the figure, the number of cassette loading/unloading ports 81 is equal to the number of main rotating disks 6 of the gene sequencing library preparation apparatus.

The number of the loading and unloading cassette openings 81 is consistent with that of the main turntables 6, so that the requirements of the cassettes 12 to be installed on the cassette integrated parts 1 of each main turntable 6 can be met, the preparation of large-batch gene sequencing libraries can be completed at one time, and the preparation efficiency of the gene sequencing libraries is improved.

In order to facilitate understanding of the specific structure and specific operation of the apparatus for preparing a gene sequencing library provided in the embodiments of the present application, a detailed description is provided below with reference to an embodiment.

Based on the foregoing description, in one embodiment, the apparatus for preparing a gene sequencing library provided in the examples of the present application may include at least one multi-freedom ultrasonic breaking unit 21, at least one multi-freedom fluorescent quantitative detection unit 31, a plurality of cartridge integrated units 1, a main turntable 6, a control system, a housing 8, and a control panel 51.

The cartridge integrated member 1 including a plurality of components such as the cartridge mounting groove 111, the cartridge locking module 113, the pipette pump device 115, the suction head drive 114, the thermal cap drive module 116, the heating module 117, the cartridge loading and unloading module 112, etc. is uniformly distributed on the main turn plate 6, and the cartridge integrated member 1 is mounted in the cartridge mounting groove 111 on the main turn plate 6 through the mounting plate thereon, so that the cartridge integrated member 1 can rotate with the main turn plate 6. The main turntable 6, control system, ultrasound module 2 and fluorescence module 3 may be mounted on a housing 8.

The sample application tip in the cartridge 12 can move freely, and the pipetting action among a plurality of test tubes in the cartridge 12 is realized (in this embodiment, the test tubes in the cartridge 12 include a sample tube, a fluorescence quantifying tube 122, and a breaking tube 121).

In specific implementation, the control system receives a card box installation instruction, drives the main turntable 6 to rotate the corresponding card box integration member 1 to the position of the card box opening 81 for loading and unloading, when the card box detection member 4 (sensor) on the card box integration member 1 detects that the card box 12 is positioned at the notch position of the card box installation groove 111, the control card box loading and unloading module 112 sucks the card box 12 into the card box installation groove 111, after the card box 12 reaches a preset position, the card box locking module 113 locks the card box 12 in the card box installation groove 111, and the gene sequencing library preparation device starts library building.

Pipette driving module 114 and the cooperation of liquid-transfering pump device 115 realize the liquid-transfering operation of application of sample pipette in card box 12, the experiment sample that will break puts into breaking pipe 121 in card box 12, control system breaks the instruction according to the supersound of acquireing, control rotating electrical machines drives main rotary table 6 and rotates this card box integrated component 1 to the supersound station, supersound module 2 breaks part 21 with the supersound and removes the position that breaks pipe 121 in this card box 12, and the completion breaks the processing of experimenting the sample in pipe 121 in this card box 12, break after finishing, supersound module 2 breaks part 21 and removes the resetting, the supersound of experiment sample breaks the completion in this card box 12, obtain breaking the experiment sample.

According to the experiment requirement, the experiment sample needing fluorescence quantification is added into the fluorescence quantification tube 122 in the card box 12 through the cooperation of the suction head driving module 114 and the liquid-transfer pump device 115, the control system controls the rotating motor to drive the main rotating disc 6 to rotate the card box integration member 1 to a fluorescence quantification station according to the obtained quantitative detection instruction, the fluorescence module 3 moves the fluorescence quantification fixing seat 32 (shown in figure 8) to the position of the fluorescence quantification tube 122 of the card box 12 and completes the fluorescence quantification of the experiment sample in the fluorescence quantification tube 122 of the card box 12, after the fluorescence quantification is finished, the fluorescence module 3 moves the fluorescence quantification fixing seat 32 away and resets, the fluorescence quantification of the fluorescence quantification tube 122 of the card box 12 is completed, and each card box 12 is provided with not less than 2 fluorescence quantification tubes 122 for fluorescence quantification of the sample in the card box 12.

According to the gene sequencing library preparation process, after a sample is built in the card box 12, the control system drives the main rotary disc 6 to rotate the card box integration member 1 to the position of the card box loading and unloading opening 81, the card box locking module 113 unlocks the card box 12 in the card box installation groove 111, the card box loading and unloading module 112 sends the card box 12 out of the card box installation groove 111, and the gene sequencing library preparation device completes one-time library building.

Although the embodiments of the present invention are disclosed above, the present invention is not limited thereto. Various changes and modifications may be effected therein by one of ordinary skill in the pertinent art without departing from the scope or spirit of the present invention, and the scope of the present invention is defined by the appended claims.

Claims

1. A gene sequencing library preparation apparatus, comprising:

the card box detection part is arranged on the card box bracket and is suitable for detecting the installation position of the card box;

2. The apparatus for preparing a gene sequencing library of claim 1, further comprising:

3. The apparatus of claim 2, wherein the cartridge comprises a sample tube and a break tube, and the ultrasound module is adapted to break an experimental sample in the cartridge, and obtaining the broken experimental sample comprises:

the control system is further adapted to adjust the relative positions of the cartridge integration part and the ultrasonic break-off part according to the acquired ultrasonic break-off instruction, so as to break off the experimental sample in the cartridge, and comprises: according to the obtained ultrasonic breaking instruction, the experimental sample in the sample tube is moved into the breaking tube, and the relative positions of the card box integration piece and the ultrasonic breaking part are adjusted to break the experimental sample in the breaking tube.

4. The apparatus of claim 1, wherein the cartridge further comprises a fluorescence quantification tube, and the fluorescence module adapted to perform concentration detection on the test sample in the cartridge comprises:

the control system, adapted to adjust the relative positions of the cartridge integrated component and the fluorescent quantitative detection component according to the obtained quantitative detection instruction, so as to detect the concentration of the experimental sample, comprises: and according to the obtained quantitative detection instruction, putting the experimental sample into the fluorescence quantitative tube, and adjusting the relative positions of the card box integration piece and the fluorescence quantitative detection part so as to detect the concentration of the experimental sample.

5. The apparatus for preparing a gene sequencing library of claim 1, further comprising:

a rotating electrical machine including a motor output shaft;

the main turntable is connected with the motor output shaft and is provided with a mounting groove for mounting the card box integrated piece; the control system is suitable for controlling the card box bracket according to the acquired card box installation instruction and the signal of the card box detection part, installing the card box, and adjusting the relative positions of the card box integration part and the fluorescence quantitative detection part according to the acquired quantitative detection instruction so as to detect the concentration of the experimental sample, and comprises:

controlling the rotating motor to drive a card box bracket arranged on the main rotary disc to rotate to a card box loading and unloading position according to the acquired card box installation instruction, and controlling the card box bracket to install the card box according to a signal of the card box detection piece;

according to the obtained quantitative detection instruction, the rotating motor is controlled to drive the card box integrated piece arranged on the main rotating disc to rotate to a quantitative detection position, and the relative positions of the card box integrated piece and the fluorescence quantitative detection part are adjusted to detect the concentration of the experimental sample.

6. The apparatus for preparing a gene sequencing library of claim 5, further comprising:

the main rotating shaft is connected with the output shaft of the motor;

the main turntable includes:

the first main rotating disc is sleeved on the main rotating shaft;

7. The apparatus of claim 1, wherein the cartridge comprises a closed cartridge.

8. The apparatus for preparing a gene sequencing library of claim 1, wherein the cassette integration unit comprises:

9. The apparatus of claim 7, wherein the cartridge further comprises a sample application tip, and the cartridge assembly further comprises:

10. The gene sequencing library preparation device of any one of claims 1 to 9, wherein the cartridge further comprises a thermal cap for controlling the temperature of a sample tube in the cartridge, and the cartridge assembly further comprises:

11. The apparatus of claim 10, wherein the thermal lid drive module comprises:

12. The apparatus for preparing a gene sequencing library of any one of claims 1 to 9, wherein the cassette assembly further comprises:

13. The apparatus for preparing a gene sequencing library of any one of claims 1-9, further comprising:

14. The apparatus for preparing a gene sequencing library of any one of claims 1-9, wherein said fluorescence module comprises:

15. The apparatus of any of claims 2 and 3, wherein the ultrasound module comprises:

a fifth direction breaking drive adapted to drive the ultrasonic breaking member to move in a fourth direction, the fifth direction being perpendicular to the fourth direction;