CN218689598U - Integrated micro-fluidic chip - Google Patents
Integrated micro-fluidic chip Download PDFInfo
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- CN218689598U CN218689598U CN202223160252.XU CN202223160252U CN218689598U CN 218689598 U CN218689598 U CN 218689598U CN 202223160252 U CN202223160252 U CN 202223160252U CN 218689598 U CN218689598 U CN 218689598U
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Abstract
The utility model relates to a bioengineering technical field discloses a micro-fluidic chip of integrated form. The integrated microfluidic chip comprises a reagent exchange unit and a working unit which are attached in a water-tight manner, wherein the upper surface of the reagent exchange unit is provided with a conventional reagent tank, a reagent exchange tank, a product tank and a waste liquid tank, and the lower surface of the reagent exchange unit is provided with a micro-channel and a groove; the conventional reagent tank is communicated with the reagent exchange tank through a micro-flow channel, and the groove is communicated with the reagent exchange tank, the product tank and the waste liquid tank through the micro-flow channel; the working unit covers the micro-channel and the groove and forms a working area with the groove; the upper surface of the reagent exchange unit is also provided with a reverse transcription reagent groove which is communicated with the product groove through a micro-channel. The utility model provides an integrated form micro-fluidic chip can pass through the mode of taking out the pressure or pressurizeing, lets the mixture of the product in the product groove and the reverse transcription reagent in the reverse transcription reagent groove back shake after, and the reverse transcription process is accomplished in the abundant reaction.
Description
Technical Field
The utility model relates to a bioengineering technical field especially relates to an integrated form micro-fluidic chip.
Background
The biochip is prepared by sequentially solidifying a large amount of biological macromolecules, such as nucleic acid fragments, polypeptide molecules, tissue slices, cells and other biological samples, on the surface of a support by adopting methods such as light guide in-situ synthesis or micro-spotting and the like to form dense two-dimensional molecular arrangement, hybridizing with target molecules in a marked biological sample to be detected, and rapidly, parallelly and efficiently detecting and analyzing the intensity of a hybridization signal through a specific instrument so as to judge the number of the target molecules in the sample.
In the prior art, biochips comprising reaction vessels such as reagent vessels, exchange vessels, product vessels, etc. have been disclosed, but microfluidic chip devices that integrate automated nucleic acid extraction and transcriptome reverse transcription reactions have been lacking. In the specific operation process, after nucleic acid is generally extracted, the nucleic acid needs to be independently placed on a special instrument to perform a reverse transcription process by constant-temperature oscillation, so that the operation is complex, and the reagent transfer process also increases the possibility of reagent pollution.
SUMMERY OF THE UTILITY MODEL
Based on above, the utility model aims to provide a can accomplish the buffer solution and wash and the micro-fluidic chip of integrated form of reverse transcription reaction after nucleic acid extraction accomplishes.
To achieve the purpose, the utility model adopts the following technical proposal:
the integrated microfluidic chip comprises a reagent exchange unit and a working unit which are attached in a watertight manner, wherein a conventional reagent tank, a reagent exchange tank, a product tank and a waste liquid tank are arranged on the upper surface of the reagent exchange unit, and a micro-channel and a groove are formed in the lower surface of the reagent exchange unit; the conventional reagent tank and the reagent exchange tank are communicated through the micro-channel, and the groove is communicated with the reagent exchange tank, the product tank and the waste liquid tank through the micro-channel; the working unit covers the micro-channel and the groove and forms a working area with the groove;
the upper surface of the reagent exchange unit is also provided with a reverse transcription reagent groove which is communicated with the product groove through the micro-channel.
As an alternative to the integrated microfluidic chip, the reagent exchange unit includes a substrate layer and an intermediate layer, the conventional reagent tank, the reagent exchange tank, the product tank, the waste tank and the reverse transcription reagent tank are disposed on an upper surface of the substrate layer, and the micro flow channel and the groove are disposed on a lower surface of the intermediate layer.
As an alternative to an integrated microfluidic chip, the intermediate layer is provided with a resistive heater located below the product and reverse transcription reagent reservoirs.
As an alternative to an integrated microfluidic chip, the intermediate layer is provided with a resistance temperature sensor located below the product and reverse transcription reagent reservoirs.
As an alternative of the integrated microfluidic chip, a capacitive sensor is arranged on the intermediate layer, and the capacitive sensor is arranged below the working interval and connected with the working interval.
As an alternative to the integrated microfluidic chip, the surface of the groove is a hydrophilic layer; the surface of the micro-channel is a hydrophobic layer;
the inner surfaces of the conventional reagent reservoir, the reagent exchange reservoir, the product reservoir, the waste reservoir and the reverse transcription reagent reservoir are hydrophobic.
As an alternative to an integrated microfluidic chip, the hydrophobic layer is formed by a hydrophobic coating, a hydrophobic material, or a surface hydrophobic treatment; the hydrophilic layer is in the same structure as the hydrophobic layer.
As an alternative to an integrated microfluidic chip, the bottoms of the reverse transcription reagent and product reservoirs are both conical; the conical bottom end is provided with a through hole communicated with the micro-channel.
As an alternative to an integrated microfluidic chip, the reverse transcription reagent reservoir communicates with the product reservoir via two of the microchannels.
As an alternative to an integrated microfluidic chip, the product and reverse transcription reagent reservoirs are fixedly connected.
The utility model has the advantages that:
the utility model provides an integrated form micro-fluidic chip has add reverse transcription reagent groove, and the result groove on the chip is linked together through the miniflow channel with reverse transcription reagent groove, during the use, can pass through the mode of withholding or pressor, lets the result of result inslot and the reverse transcription reagent in the reverse transcription reagent inslot mix and back the vibration afterwards, and the reverse transcription process is accomplished in the abundant reaction.
Drawings
Fig. 1 is a schematic view of an overall structure of an integrated microfluidic chip according to a first view angle;
fig. 2 is a schematic view of an overall structure of the integrated microfluidic chip at a second viewing angle;
fig. 3 is a top view of the integrated microfluidic chip provided in the present invention;
fig. 4 is a schematic diagram of a bottom structure of an intermediate layer of the integrated microfluidic chip provided by the present invention;
fig. 5 is a perspective view of an integrated microfluidic chip provided by the present invention;
fig. 6 is a schematic diagram of a top structure of an intermediate layer of the integrated microfluidic chip according to the present invention;
fig. 7 is an enlarged view of a portion of the resistance heater and resistance temperature sensor of the integrated microfluidic chip provided by the present invention;
fig. 8 is an enlarged view of a capacitive sensor portion of the integrated microfluidic chip according to the present invention.
In the figure:
100-a reagent exchange unit; 110-a base layer; 120-an intermediate layer; 121-resistive heater; 122-a resistance temperature sensor; 123-a capacitive sensor;
101-a product tank; 102-reverse transcription reagent tank; 103-conventional reagent tank; 104-a reagent exchange tank; 105-a waste liquid tank; 106-working interval; 107-micro flow channel;
200-a working unit.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention. It should be further noted that, for the convenience of description, only some of the structures related to the present invention are shown in the drawings, not all of the structures.
In the description of the present invention, unless expressly stated or limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, e.g., as meaning permanently connected, detachably connected, or integral to one another; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
In the description of the present embodiment, the terms "upper", "lower", "right", etc. are used in an orientation or positional relationship based on that shown in the drawings only for convenience of description and simplicity of operation, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used only for descriptive purposes and are not intended to have a special meaning.
The embodiment provides an integrated micro-fluidic chip, adds reverse transcription reagent groove on the basis of current micro-fluidic chip, increases the function of reverse transcription experiment for this integrated micro-fluidic chip to solve current micro-fluidic chip after nucleic acid extraction is accomplished, need to shift the experiment result to carry out reverse transcription reaction on other instruments, operation process is comparatively complicated, and the reagent easily receives the problem of pollution.
Referring to fig. 1 to 5, the integrated microfluidic chip provided in this embodiment includes a reagent exchange unit 100 and a working unit 200 which are attached to each other in a water-tight manner, wherein a cup structure including a conventional reagent tank 103, a reagent exchange tank 104, a product tank 101, and a waste liquid tank 105 is disposed on an upper surface of the reagent exchange unit 100, a micro channel 107 and a groove are disposed on a lower surface of the reagent exchange unit 100, and the cup structure is communicated with the micro channel 107 through a through hole disposed on the reagent exchange unit 100; the conventional reagent tank 103 and the reagent exchange tank 104 are communicated through a micro flow channel 107, and the groove is communicated with the reagent exchange tank 104, the product tank 101 and the waste liquid tank 105 through the micro flow channel 107; the working unit 200 covers the micro flow channel 107 and the groove, and forms a working space 106 with the groove for the reaction reagent to mix and react.
In the experiment, the required reagent is pressurized, the reagent flows to the reagent exchange tank 104 along the micro flow channel 107 at the bottom of the reagent exchange unit 100, and then the reaction reagent in the reagent exchange tank 104 flows into the working space 106 by pumping the waste liquid tank 105 or the product tank 101.
In the integrated microfluidic chip provided in this embodiment, the reagent exchange unit 100 is further provided with a reverse transcription reagent tank 102 on the upper surface, and the reverse transcription reagent tank 102 is communicated with the product tank 101 through a micro channel 107.
The experimental process comprises the following steps:
1. according to the requirement, reagents required by the reaction, such as cell samples, flushing liquid, lysate, molecular markers, reverse transcription reagents and the like, are put into the corresponding grooves of the reagent exchange unit 100;
2. enabling the cell sample to enter the working area 106, enabling the working unit 200 to capture single cells, enabling the flushing liquid to enter the working area 106, and flushing unnecessary impurity molecules;
3. the molecular marker enters a working interval 106, and each single cell is marked;
4. the lysate enters the working space 106 to extract the nucleic acid in the cells, then the buffer enters the working space 106 to wash unnecessary impurity molecules, and the working space 106 is filled with liquid to facilitate the collection of the product;
5. the product is recovered into the product tank 101, and the product is mixed with the reverse transcription reagent in the reverse transcription reagent tank 102 by means of pressure pumping or pressurization, and then the mixture is vibrated back to react fully, so that the reverse transcription process is completed.
When the integrated microfluidic chip provided in this embodiment is used, the reverse transcription reagent tank 102 is additionally provided, and the micro flow channel 107 is used to communicate the product tank 101 and the reverse transcription reagent tank 102, so that when the integrated microfluidic chip is used, the product in the product tank 101 and the reverse transcription reagent in the reverse transcription reagent tank 102 can be mixed by pumping or pressurizing, and then oscillated back, thereby fully reacting to complete the reverse transcription process.
Preferably, in this embodiment, the reverse transcription reagent tank 102 is connected to the product tank 101 through two micro flow channels 107 to avoid blockage, so that the oscillation process can be smoothly performed; meanwhile, the product tank 101 and the reverse transcription reagent tank 102 are fixedly connected to improve the mechanical strength of the two, and the product tank 101 and the reverse transcription reagent tank 102 are prevented from being deformed or damaged due to frequent pressurization or pressure extraction.
Furthermore, in this embodiment, the bottoms of the reverse transcription reagent tank 102 and the product tank 101 are both conical, and the conical bottoms are both provided with through holes communicated with the micro flow channel 107, so as to avoid liquid residues in the reverse transcription reagent tank 102 and the product tank 101, and ensure sufficient mixing and oscillation of the product and the reverse transcription reagent.
With continued reference to FIGS. 1 and 2, in the present embodiment, the reagent exchange unit 100 includes a base layer 110 and an intermediate layer 120 which are attached to each other in a watertight manner, cup structures such as a normal reagent tank 103, a reagent exchange tank 104, a product tank 101, a waste liquid tank 105, and a reverse transcription reagent tank 102 are disposed on the upper surface of the base layer 110, and a micro flow channel 107 and a groove are disposed on the lower surface of the intermediate layer 120. The purpose of this design is to allow for the replacement and assembly of the intermediate layer 120 during the manufacturing process, depending on the different requirements of the micro flow channel 107 for different products.
In this embodiment, the surface of recess is hydrophilic layer, and the surface of microchannel 107 is the hydrophobic layer, and the internal surface of cup structure is the hydrophobic layer. The hydrophobic layer may be formed by a hydrophobic coating, a hydrophobic material or a surface hydrophobic treatment, the hydrophilic layer being in the same order as the hydrophobic layer. For example, a hydrophilic coating may be disposed on the surface of the groove, a hydrophobic coating may be disposed on the surface of the micro flow channel 107, and the cup structure may be formed by injection molding of a hydrophobic material.
Set up the hydrophobic layer on the surface of cup and microchannel 107 and can prevent that reagent from appearing remaining when shifting, set up hydrophilic layer in the recess and can make more abundant that reagent diffuses in working interval 106, avoided the production of bubble, guarantee the accuracy that detects.
Referring to fig. 6 to 8, in the integrated microfluidic chip provided in this embodiment, the middle layer 120 is provided with a resistive heater 121, a resistive temperature sensor 122, and a capacitive sensor 123, wherein: the resistance heater 121 is positioned below the product tank 101 and the reverse transcription reagent tank 102, and heats the reagents in the product tank 101 and the reverse transcription reagent tank 102; the resistance temperature sensor 122 is positioned below the product tank 101 and the reverse transcription reagent tank 102 and feeds back the temperature; the capacitance sensor 123 is disposed below the operating section 106, is in communication with the liquid in the operating section 106, and feeds back the liquid and the gas in the operating section 106 by a change in capacitance value due to a difference in dielectric constant ∈ r between the liquid and the gas.
Specifically, in this embodiment, the resistive heater 121, the resistive temperature sensor 122 and the capacitive sensor 123 can be prepared by sputtering and patterning by a method provided in the patent-a biochip with a temperature control matrix and a processing method thereof-CN 101086009. Of course, other existing devices, such as those manufactured by thin film technology, micro-ultra technology, etc., specifically, the thin film resistive heater 121, the thin film resistive temperature sensor 122, the micro-ultra capacitive sensor 123, etc., may also be used to achieve the desired effects of the present patent.
The heating by the resistance heater 121, the monitoring of the temperature by the resistance temperature sensor 122, the control of the working conditions of the resistance heater 121 and the resistance temperature sensor 122 by the control circuit to realize the constant temperature function, and the monitoring of the gas-liquid condition or the liquid level in the container by the capacitance sensor 123 are the prior art, and are not described herein again.
The integrated microfluidic chip provided by this embodiment can monitor the temperatures of the product tank 101 and the reverse transcription reagent tank 102 through the resistance heater 121 and the resistance temperature sensor 122 to satisfy the constant temperature heating condition of the reverse transcription reaction, ensure the accuracy of the detection result, and feed back the gas-liquid condition in the working interval 106 to the experimental instrument through the capacitance sensor 123, so that the detection process can be performed automatically.
Note: the fixing means not explicitly described herein may be a conventional fixing means such as a screw connection, a welding or an adhesive connection, as required.
It is obvious that the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Numerous obvious variations, rearrangements and substitutions will now occur to those skilled in the art without departing from the scope of the invention. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (10)
1. The integrated micro-fluidic chip comprises a reagent exchange unit (100) and a working unit (200) which are attached in a water-tight manner, wherein a conventional reagent tank (103), a reagent exchange tank (104), a product tank (101) and a waste liquid tank (105) are arranged on the upper surface of the reagent exchange unit (100), and a micro-channel (107) and a groove are formed in the lower surface of the reagent exchange unit (100); the conventional reagent tank (103) and the reagent exchange tank (104) are communicated through the micro flow channel (107), and the groove is communicated with the reagent exchange tank (104), the product tank (101) and the waste liquid tank (105) through the micro flow channel (107); the working unit (200) covers the micro-channel (107) and the groove and forms a working area (106) with the groove;
the method is characterized in that: the upper surface of the reagent exchange unit (100) is also provided with a reverse transcription reagent groove (102), and the reverse transcription reagent groove (102) is communicated with the product groove (101) through the micro-channel (107).
2. The integrated microfluidic chip according to claim 1, wherein the reagent exchange unit (100) comprises a water-tight base layer (110) and an intermediate layer (120), the conventional reagent tank (103), the reagent exchange tank (104), the product tank (101), the waste liquid tank (105), and the reverse transcription reagent tank (102) are disposed on an upper surface of the base layer (110), and the micro flow channel (107) and the groove are disposed on a lower surface of the intermediate layer (120).
3. The integrated microfluidic chip according to claim 2, wherein a resistive heater (121) is disposed on the intermediate layer (120), the resistive heater (121) being located below the product reservoir (101) and the reverse transcription reagent reservoir (102).
4. The integrated microfluidic chip according to claim 2, wherein a resistive temperature sensor (122) is disposed on the intermediate layer (120), the resistive temperature sensor (122) being located below the product reservoir (101) and the reverse transcription reagent reservoir (102).
5. The integrated microfluidic chip according to claim 2, wherein a capacitive sensor (123) is arranged on the intermediate layer (120), and the capacitive sensor (123) is arranged below the working space (106) and connected to the working space (106).
6. The integrated microfluidic chip according to claim 1, wherein the surface of the groove is a hydrophilic layer; the surface of the micro-channel (107) is a hydrophobic layer;
the inner surfaces of the normal reagent tank (103), the reagent exchange tank (104), the product tank (101), the waste liquid tank (105), and the reverse transcription reagent tank (102) are hydrophobic.
7. The integrated microfluidic chip according to claim 6, wherein said hydrophobic layer is formed by a hydrophobic coating, a hydrophobic material or a surface hydrophobic treatment; the hydrophilic layer is in the same order as the hydrophobic layer.
8. The integrated microfluidic chip according to claim 1, wherein the bottoms of the reverse transcription reagent tank (102) and the product tank (101) are both conical; the conical bottom end is provided with a through hole communicated with the micro-channel (107).
9. The integrated microfluidic chip according to claim 1, wherein the reverse transcription reagent reservoir (102) communicates with the product reservoir (101) via two microchannels (107).
10. The integrated microfluidic chip according to claim 1, wherein the product reservoir (101) and the reverse transcription reagent reservoir (102) are fixedly connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223160252.XU CN218689598U (en) | 2022-11-28 | 2022-11-28 | Integrated micro-fluidic chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223160252.XU CN218689598U (en) | 2022-11-28 | 2022-11-28 | Integrated micro-fluidic chip |
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| CN218689598U true CN218689598U (en) | 2023-03-24 |
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| CN202223160252.XU Active CN218689598U (en) | 2022-11-28 | 2022-11-28 | Integrated micro-fluidic chip |
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