CN218666025U - Novel extracellular matrix extraction device - Google Patents
Novel extracellular matrix extraction device Download PDFInfo
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- CN218666025U CN218666025U CN202223211382.1U CN202223211382U CN218666025U CN 218666025 U CN218666025 U CN 218666025U CN 202223211382 U CN202223211382 U CN 202223211382U CN 218666025 U CN218666025 U CN 218666025U
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Abstract
The utility model relates to an extracellular matrix extraction element field discloses a novel extracellular matrix extraction element, including drawing the bucket, the front end of drawing the bucket upper surface is provided with the inlet pipe, draw the first mounting box of rear end fixedly connected with of bucket upper surface, the one side of drawing the bucket upper surface is provided with the medicament entry, draw the lower extreme fixedly connected with fluid-discharge tube of bucket one side outer wall, draw the inside upper end of bucket and seted up the water storage chamber, draw the inside lower extreme of bucket and seted up the working chamber, draw the inside of bucket outer wall and seted up the chamber of ventilating, draw the center department fixedly connected with electric telescopic handle of bucket upper surface. The utility model discloses in, the contrast is current device, and the device can start the hot plate when drawing and keep the best extraction temperature to whole bucket that draws through the hot plate that sets up, probably because of the low or high death that leads to the cell of temperature excessively when can effectually avoiding drawing.
Description
Technical Field
The utility model relates to an extracellular matrix extraction element field especially relates to a novel extracellular matrix extraction element.
Background
The cell types are various, different cell types have different cell matrixes, wherein stem cells are cells with unlimited or immortal self-renewal capacity, can generate at least one type of highly differentiated progeny cells, the definition of the stem cells is continuously corrected for many years, and the extracellular matrix is defined from different levels and mainly consists of 5 types of substances, namely collagen, non-collagen, elastin, proteoglycan and aminoglycan which are mainly divided into a basement membrane and an interstitial matrix according to distribution parts.
Most of the existing extraction devices can not effectively control the temperature of the extraction device in the extraction process of the extracellular matrix layer of the cells, the cell death can be caused by the too low or too high temperature of the extraction device, the extraction devices can not be effectively cleaned after the extraction is finished, and the extraction reagent and the raw materials can not be fully fused in the extraction process, so that the technical improvement is urgently needed.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the shortcoming that exists among the prior art, and the novel extracellular matrix extraction element who proposes, the current device of contrast, the device is through the water storage chamber that sets up, after extracting that the whole work of bucket finishes, accessible injection valve erupts the messenger of water storage intracavity, wash the inner wall of working chamber with this, then start electric telescopic handle, make electric telescopic handle fall servo motor, servo motor drives the carousel and stirs the post rotation, thereby it rotates to drive the rivers of working intracavity, thereby make rivers can be better reach the abluent effect of working chamber inner wall.
In order to achieve the above purpose, the utility model provides a following technical scheme: a novel extracellular matrix extraction device comprises an extraction barrel with a hollow interior, wherein an inner cavity of the extraction barrel is divided into a ventilation cavity and a sealed cavity, the ventilation cavity is formed between the sealed cavity and the outer wall of the extraction barrel, a water storage cavity and a working cavity are formed in the sealed cavity through a partition plate, the water storage cavity is positioned above the working cavity, a feed pipe, a first mounting box and a medicament inlet are arranged at the top end of the extraction barrel, the feed pipe is communicated with the working cavity after passing through the ventilation cavity and the water storage cavity, the medicament inlet is communicated with the water storage cavity, and a liquid discharge pipe is arranged on the outer wall of the extraction barrel and is communicated with the working cavity;
an electric telescopic rod is fixedly connected to the center of the top of the extraction barrel, the output end of the electric telescopic rod penetrates through the extraction barrel and is fixedly connected with a second mounting box, the second mounting box is located in the water storage cavity and is fixed with the extraction barrel and the partition plate, the bottom of the second mounting box penetrates through the partition plate, a servo motor is fixedly connected to the inner top surface of the second mounting box, the output end of the servo motor penetrates through the second mounting box and is fixedly connected with a rotary table, a plurality of stirring columns are fixedly connected to the lower surface of the rotary table, and the rotary table and the stirring columns are located in the working cavity;
the interior bottom surface fixedly connected with air-blower of first mounting box, the output of air-blower runs through first mounting box, draws the bucket to the chamber of ventilating, ventilate a plurality of gas outlets of the bottom upper surface through connection in chamber, gas outlet and working chamber intercommunication are provided with the check valve in the gas outlet, draw bucket outer wall fixedly connected with water intaking valve, water intaking valve and water storage chamber through connection, are provided with a plurality of injection valves on the baffle, and the injection valve communicates with water storage chamber and working chamber simultaneously, the equal fixedly connected with hot plate of both sides inner wall of working chamber, the fixedly connected with flowing back valve is located at the center of working chamber lower surface.
Through above-mentioned technical scheme, the current device of contrast, the device is through the air-blower that sets up, when putting into the working chamber to drawing reagent and raw and other materials, the accessible starts the air-blower, and the air-blower inhales outside wind to from the gas outlet blowout, make gas outlet spun gas can carry out better integration with drawing reagent and raw and other materials, with this promote the extraction speed on the outer matrix layer, the check valve that sets up moreover can prevent that liquid from flowing backwards and ventilate the chamber.
Furthermore, a plurality of supporting legs are fixedly connected to the lower surface of the extraction barrel;
through the technical scheme: the lower surface of the extraction barrel is fixedly connected with a plurality of supporting legs, so that the extraction barrel can be supported.
Furthermore, the lower surfaces of the supporting legs are fixedly connected with supporting pads;
through above-mentioned technical scheme, see the lifting surface area of effectual increase supporting leg to workspace ground through the equal fixedly connected with supporting pad of lower surface of supporting leg.
Furthermore, a filter screen is arranged on the outer wall of one side of the first mounting box;
through above-mentioned technical scheme, one side outer wall through first mounting box is provided with the impurity in the filter screen can be better gas and filters.
Furthermore, a disc body of the turntable and a column body of the stirring column are both provided with a plurality of holes;
through above-mentioned technical scheme, the shaft of the dish through the carousel and the shaft of stirring post have all seted up the drive rivers that a plurality of holes can be better and have washd the working chamber.
Further, the upper end of the feeding pipe is in threaded connection with a sealing cover;
through above-mentioned technical scheme, there is the purity of assurance raw and other materials that the closing cap can be better through the upper end threaded connection of inlet pipe.
Further, the upper end of the medicament inlet is in threaded connection with a threaded cover;
through the technical scheme, the upper end of the medicament inlet is in threaded connection with the threaded cover, so that raw materials or extracted reagents are effectively prevented from overflowing during working.
Furthermore, an observation window is arranged on the outer wall of one side of the extraction barrel, which is far away from the liquid discharge pipe;
through the technical scheme, the outer wall of one side of the extraction barrel, which is far away from the liquid discharge pipe, is provided with the observation window, so that the working condition of the extraction barrel can be observed more conveniently.
The utility model discloses following beneficial effect has:
1. the utility model provides a novel extracellular matrix extraction device compares current device, and the device can start the hot plate when drawing and keep the best temperature of drawing to whole bucket that draws through the hot plate that sets up, probably because of the temperature is low excessively or the high death that leads to the cell when can effectually avoiding drawing.
2. The utility model provides a novel extracellular matrix extraction device, compare current device, the device is through the water storage chamber that sets up, after extracting that the whole work of bucket finishes, accessible injection valve erupts the messenger of water storage intracavity to this washs the inner wall of working chamber, then starts electric telescopic handle, make electric telescopic handle fall servo motor, servo motor drives the carousel and stirs the post rotation, thereby it rotates to drive the rivers of working chamber, thereby make rivers can be better reach the abluent effect of working chamber inner wall.
3. The utility model provides a novel extracellular matrix extraction device, the current device of contrast, the device is through the air-blower that sets up, when putting into the working chamber extracting reagent and raw and other materials, the accessible starts the air-blower, and the air-blower inhales outside wind to from the gas outlet blowout, make gas outlet spun gas can carry out better integration with raw and other materials extracting reagent, with this promote the extraction rate on external matrix layer.
Drawings
FIG. 1 is an isometric view of a novel extracellular matrix extraction device of the present invention;
FIG. 2 is a front sectional view of a novel extracellular matrix extraction device provided by the present invention;
FIG. 3 is an enlarged view taken at A in FIG. 2;
fig. 4 is an enlarged view at B in fig. 2.
Illustration of the drawings:
1. an extraction barrel; 2. a liquid discharge pipe; 3. supporting legs; 4. a support pad; 5. an observation window; 6. a first mounting box; 7. a threaded cap; 8. filtering with a screen; 9. an electric telescopic rod; 10. sealing the cover; 11. a feed pipe; 12. a water inlet valve; 13. a servo motor; 14. a stirring column; 15. a hole; 16. a vent lumen; 17. a drain valve; 18. heating plates; 19. a second mounting box; 20. a water storage cavity; 21. a blower; 22. a working chamber; 23. a turntable; 24. an injection valve; 25. an air outlet; 26. a medicament inlet.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides an embodiment: the device comprises an extraction barrel 1, wherein a feeding pipe 11 is arranged at the front end of the upper surface of the extraction barrel 1, a first mounting box 6 is fixedly connected with the rear end of the upper surface of the extraction barrel 1, a medicament inlet 26 is arranged on one side of the upper surface of the extraction barrel 1, a liquid discharge pipe 2 is fixedly connected with the lower end of the outer wall of one side of the extraction barrel 1, a water storage cavity 20 is formed in the upper end of the interior of the extraction barrel 1, a working cavity 22 is formed in the lower end of the interior of the extraction barrel 1, and a ventilation cavity 16 is formed in the interior of the outer wall of the extraction barrel 1;
the center of the upper surface of the extraction barrel 1 is fixedly connected with an electric telescopic rod 9, the output end of the electric telescopic rod 9 penetrates through the extraction barrel 1 and is fixedly connected with a second mounting box 19, the inner top surface of the second mounting box 19 is fixedly connected with a servo motor 13, the output end of the servo motor 13 penetrates through the second mounting box 19 and is fixedly connected with a turntable 23, and the lower surface of the turntable 23 is fixedly connected with a plurality of stirring columns 14;
the inner bottom surface of the first mounting box 6 is fixedly connected with an air blower 21, the output end of the air blower 21 penetrates through the first mounting box 6 and the extraction barrel 1 to the ventilation cavity 16, the upper surface of the bottom end of the ventilation cavity 16 is connected with a plurality of air outlets 25 in a penetrating manner, the upper end of the outer wall of one side of the extraction barrel 1 is fixedly connected with a water inlet valve 12, the water inlet valve 12 is connected with a water storage cavity 20 in a penetrating manner, the lower surface of the water storage cavity 20 is connected with a plurality of injection valves 24 in a penetrating manner, the inner walls of two sides of the working cavity 22 are fixedly connected with heating plates 18, and the center of the lower surface of the working cavity 22 is fixedly connected with a liquid discharge valve 17;
compare current device, the device is through the air-blower 21 that sets up, when putting into working chamber 22 extracting reagent and raw and other materials, the accessible starts air-blower 21, and air-blower 21 inhales outside wind to spout from gas outlet 25, make gas outlet 25 spun gaseous can carry out better integration with extracting reagent and raw and other materials, with this promotion to the extraction rate on outer matrix layer. In order to cope with the internal water pressure, it is conceivable to provide the air outlet 25 with a pressurizing device for increasing the pressure of the air flow, thereby enabling the air flow to be injected into the working chamber 22.
Draw a plurality of supporting legs 3 of lower fixed surface of bucket 1, through above-mentioned technical scheme: draw a plurality of supporting legs 3 of lower fixed surface of bucket 1 and can play the supporting role for this draws bucket 1, the equal fixedly connected with supporting pad 4 of lower surface of supporting leg 3, see the lifting surface area of effectual increase supporting leg 3 to workspace ground through the equal fixedly connected with supporting pad 4 of lower surface of supporting leg 3, one side outer wall of first mounting box 6 is provided with filter screen 8, it can better impurity in the gas to be provided with filter screen 8 through one side outer wall of first mounting box 6 filters, a plurality of holes 15 have all been seted up to the shaft of carousel 23 and the shaft of stirring post 14, it can better drive rivers to wash working chamber 22 to have all been seted up a plurality of holes 15 through the shaft of carousel 23 and the shaft of stirring post 14, the upper end threaded connection of inlet pipe 11 has closing cap 10, there is the pure of assurance raw and other materials that closing cap 10 can be better through the upper end threaded connection of inlet pipe 11, the upper end threaded connection of medicament inlet 26 has threaded cap 7 the effectual outer raw and other materials of avoiding the during operation or drawing reagent, draw the fluid-discharge tube 2's that one side outer wall of drawing bucket 1 keeps away from observation window 5 through drawing one side of outer wall of 1 of bucket 2 and can be provided with the observation window condition that the observation barrel 1 that makes things convenient observation barrel 1 that the observation barrel carries out.
The working principle is as follows: when the device is used, raw materials are put into the feeding pipe 11, an extraction reagent is put into the reagent inlet 26 according to the existing proportion, the heating plate 18 is started, the working cavity 22 is kept at the optimal extraction temperature, the blower 21 is started, the blower 21 filters external air and blows the air out of the air outlet 25 through the air vent cavity 16, the raw materials and the extraction reagent in the working cavity 22 are fully fused, after the fusion is completed, the upper layer is supernatant, the lower layer is insoluble, the liquid discharge valve 17 is opened, the insoluble substances are discharged through the observation window 5, then the supernatant is discharged through the liquid discharge pipe 2, the liquid discharge pipe 2 is closed, all the insoluble substances are discharged through the liquid discharge valve 17, then the liquid discharge valve 17 is closed, the water in the water storage cavity 20 is sprayed through the injection valve 24, the interior of the working cavity 22 is cleaned, when a large amount of water appears on the surface of the working cavity 22, the second mounting box 19 is lowered through the electric telescopic rod 9, the servo motor 13 in the second mounting box 19 drives the rotary table 23 and the stirring column 14 to rotate, so as to drive the water flow to clean the interior of the working cavity 22, and further open the water flow and discharge pipe 17.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.
Claims (8)
1. A novel extracellular matrix extraction device comprises an internal hollow extraction barrel (1), and is characterized in that: the inner cavity of the extraction barrel (1) is divided into a ventilation cavity (16) and a sealing cavity, the ventilation cavity (16) is arranged between the sealing cavity and the outer wall of the extraction barrel (1), a water storage cavity (20) and a working cavity (22) are formed in the sealing cavity through a partition plate, the water storage cavity (20) is located above the working cavity (22), a feeding pipe (11), a first mounting box (6) and a medicament inlet (26) are arranged at the top end of the extraction barrel (1), the feeding pipe (11) penetrates through the ventilation cavity (16) and the water storage cavity (20) and then is communicated with the working cavity (22), the medicament inlet (26) is communicated with the water storage cavity (20), a liquid discharge pipe (2) is arranged on the outer wall of the extraction barrel (1), and the liquid discharge pipe (2) is communicated with the working cavity (22);
an electric telescopic rod (9) is fixedly connected to the center of the top of the extraction barrel (1), the output end of the electric telescopic rod (9) penetrates through the extraction barrel (1) and is fixedly connected with a second mounting box (19), the second mounting box (19) is located in a water storage cavity (20) and is fixed with the extraction barrel (1) and a partition plate, the bottom of the second mounting box (19) penetrates through the partition plate, a servo motor (13) is fixedly connected to the inner top surface of the second mounting box (19), the output end of the servo motor (13) penetrates through the second mounting box (19) and is fixedly connected with a rotary table (23), a plurality of stirring columns (14) are fixedly connected to the lower surface of the rotary table (23), and the rotary table (23) and the stirring columns (14) are both located in a working cavity (22);
the interior bottom surface fixedly connected with air-blower (21) of first mounting box (6), the output of air-blower (21) runs through first mounting box (6), draws bucket (1) to ventilate chamber (16), ventilate a plurality of gas outlets (25) of bottom upper surface through connection of chamber (16), gas outlet (25) and working chamber (22) intercommunication, be provided with the check valve in gas outlet (25), draw bucket (1) outer wall fixedly connected with water intaking valve (12), water intaking valve (12) and water storage chamber (20) through connection, be provided with a plurality of injection valves (24) on the baffle, injection valve (24) communicate with water storage chamber (20) and working chamber (22) simultaneously, the equal fixedly connected with hot plate (18) of both sides inner wall of working chamber (22), fixedly connected with flowing back valve (17) are located at the center of working chamber (22) lower surface.
2. The novel extracellular matrix extraction device according to claim 1, wherein: the lower surface of the extraction barrel (1) is fixedly connected with a plurality of supporting legs (3).
3. The novel extracellular matrix extraction device according to claim 2, wherein: the lower surface of the supporting leg (3) is fixedly connected with a supporting pad (4).
4. The novel extracellular matrix extraction device according to claim 1, wherein: and a filter screen (8) is arranged on the outer wall of one side of the first mounting box (6).
5. The novel extracellular matrix extraction device according to claim 1, wherein: the disc body of the rotary disc (23) and the column body of the stirring column (14) are provided with a plurality of holes (15).
6. The novel extracellular matrix extraction device according to claim 1, wherein: the upper end of the feeding pipe (11) is connected with a sealing cover (10) in a threaded manner.
7. The novel extracellular matrix extraction device according to claim 1, wherein: the upper end of the medicament inlet (26) is in threaded connection with a threaded cover (7).
8. The novel extracellular matrix extraction device according to claim 1, wherein: the outer wall of the extraction barrel (1) is provided with an observation window (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223211382.1U CN218666025U (en) | 2022-12-01 | 2022-12-01 | Novel extracellular matrix extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223211382.1U CN218666025U (en) | 2022-12-01 | 2022-12-01 | Novel extracellular matrix extraction device |
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| Publication Number | Publication Date |
|---|---|
| CN218666025U true CN218666025U (en) | 2023-03-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223211382.1U Active CN218666025U (en) | 2022-12-01 | 2022-12-01 | Novel extracellular matrix extraction device |
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|---|---|
| CN (1) | CN218666025U (en) |
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2022
- 2022-12-01 CN CN202223211382.1U patent/CN218666025U/en active Active
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