CN218646704U

CN218646704U - Sample processing device

Info

Publication number: CN218646704U
Application number: CN202222869344.9U
Authority: CN
Inventors: 池沛冬
Original assignee: Sun Yat Sen University Cancer Center
Current assignee: Guangzhou Weimi Biological Science & Technology Co ltd
Priority date: 2022-10-27
Filing date: 2022-10-27
Publication date: 2023-03-17
Anticipated expiration: 2032-10-27

Abstract

The utility model relates to a sample processing device, including the organism to and set up stock solution box, reaction pot and the liquid receiving pipe that connects gradually in the organism, placed buffer solution or frozen stock solution in the stock solution box, the stock solution box passes through the pipeline intercommunication with the reaction pot, is equipped with the ultrasonic vibrator with adjustable frequency on the reaction pot, and the liquid receiving pipe is connected with the liquid outlet of reaction pot. Still including being used for placing the thing box of putting of tissue sample, put the thing box and place the holding intracavity at the reaction kettle, put and be equipped with a plurality of through-holes that communicate with the holding chamber on the thing box. The utility model discloses a sample processing apparatus improves the utilization ratio of cell extraction efficiency and tissue sample greatly, has replaced artifical the grinding simultaneously.

Description

Sample processing device

Technical Field

The utility model relates to a organize sample treatment facility technical field, especially relate to a sample processing apparatus.

Background

At present, the needle biopsy method is the most commonly used method for determining cancer, the needle biopsy method has the advantages that a surgical incision is not needed, but only a small amount of tissue samples can be taken by adopting puncture, and in clinical diagnosis work, physical methods such as manual grinding, shearing and the like are adopted when cells in the tissue samples are extracted.

In the method, under the condition that the lesion part is small or the lesion part is inconvenient to obtain, a coarse needle puncture specimen with a small original tissue quantity is inevitably used, so that the tissue quantity of pathological examination is insufficient in many cases, the simultaneous detection of pathological biopsy and cell typing (or immune state) cannot be realized, re-sampling is needed for examination, and the pain of a patient is aggravated; in addition, manual grinding is time-consuming and labor-consuming, the processing method is complicated in operation and time-consuming, and the integrity of cells is easily damaged by manual grinding due to the fact that great artificial factors exist in grinding force of operators during operation, so that the detection of the cells is influenced, and the accuracy of a diagnosis result is caused.

SUMMERY OF THE UTILITY MODEL

In view of the above, there is a need to provide a sample processing device, which can effectively control the cell peeling amount of a tissue sample by controlling the frequency of ultrasonic vibration and the vibration time, and can break the tissue sample to prepare a cell suspension, or peel only superficial cells of the tissue sample for subsequent pathology and cell detection. The sample processing device greatly improves the cell extraction efficiency and the utilization rate of tissue samples, and replaces manual grinding.

The technical scheme is as follows:

on one hand, the sample processing device comprises a machine body, a liquid storage box, a reaction pot and a liquid receiving pipe, wherein the liquid storage box, the reaction pot and the liquid receiving pipe are arranged in the machine body and are sequentially connected, a buffer solution or a frozen solution is placed in the liquid storage box, the liquid storage box is communicated with the reaction pot through a pipeline, an ultrasonic vibrator with adjustable frequency is arranged on the reaction pot, and the liquid receiving pipe is connected with a liquid outlet of the reaction pot;

still including being used for placing the thing box of putting of tissue sample, it places to put the thing box the holding intracavity of reaction kettle, put be equipped with on the thing box a plurality of with the through-hole of holding chamber intercommunication.

The technical solution is further explained as follows:

in one embodiment, an operating platform is arranged on the machine body, and the reaction pot and the liquid receiving pipe are placed on the operating platform.

In one embodiment, the operating platform is provided with a liquid receiving frame, a placing cavity for placing the liquid receiving pipe is arranged in the liquid receiving frame, and the upper end of the liquid receiving frame is provided with an opening which is gradually inclined from inside to outside.

In one embodiment, the reaction kettle further comprises a waste liquid box, and the waste liquid box is connected with the liquid outlet of the reaction kettle.

In one embodiment, the liquid receiving frame is arranged between the reaction pot and the waste liquid box, one end of the placing cavity is connected with a liquid outlet of the reaction pot, and a liquid outlet hole communicated with the waste liquid box is formed in the bottom of the placing cavity.

In one embodiment, the machine body is further provided with a protective cover for sealing the operating platform, and one end of the protective cover is hinged to the machine body.

In one embodiment, the machine body is provided with a storage box for storing the liquid storage box, the storage box is provided with a closed door, the closed door is hinged with the machine body through a rotating shaft, and one or more liquid storage boxes are arranged.

In one embodiment, the device further comprises a plurality of driving mechanisms for driving liquid to flow among the liquid storage box, the reaction pot and the liquid receiving pipe, and the driving mechanisms are respectively arranged between the liquid storage box and the reaction pot and between the reaction pot and the liquid receiving pipe.

In one embodiment, the drive mechanism is configured as a peristaltic pump.

In one embodiment, a liquid outlet pipe is arranged between the reaction pot and the liquid receiving pipe, a liquid outlet of the liquid outlet pipe is arranged above the liquid receiving pipe, and the liquid outlet pipe is telescopically arranged in the machine body.

The utility model has the advantages that:

1. by utilizing the ultrasonic cavitation technology, the tissue sample can be completely crushed or the superficial layer cells can be crushed and peeled layer by layer. The whole broken tissue can be directly prepared into cell suspension; the tissue with the surface layer cells peeled off and the remaining undamaged tissue can be subjected to pathological detection, and the peeled cells can be subjected to subsequent cell detection, so that the utilization rate of a tissue sample is improved.

2. Through utilizing this sample processing apparatus to improve cell extraction efficiency greatly, reduce artifical intensity of labour simultaneously.

3. In the case where the lesion site is small or the lesion site is not easily obtained, simultaneous detection of pathological biopsy and cell typing (or immune status) cannot be achieved. The technology can reduce the waste of tissue samples and the wound of patients caused by obtaining the tissue samples from the bodies of the patients.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention and do not constitute a limitation on the invention.

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a schematic diagram of the schematic structure of a sample processing device in one embodiment;

FIG. 2 is a schematic diagram of the structure of the housing in one embodiment;

FIG. 3 is a schematic top view of the housing in one embodiment;

FIG. 4 is a schematic diagram of an embodiment of a storage compartment;

figure 5 is a schematic diagram of an embodiment of an effluent channel.

Description of reference numerals:

100. a body; 110. an operating platform; 120. a liquid receiving frame; 121. a placement chamber; 122. a liquid outlet hole; 123. a connecting plate; 124. a chute; 130. a protective cover; 140. a storage box; 141. a closing door; 142. a rotating shaft; 150. a touch screen; 200. a liquid storage box; 300. a reaction pot; 310. an ultrasonic vibrator; 400. a liquid receiving pipe; 500. a storage box; 510. a through hole; 520. a box cover; 530. a connecting portion; 600. a waste liquid box; 700. a peristaltic pump; 800. a liquid outlet pipe; 810. a slide block.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The invention may be embodied in many other forms different from those described herein and similar modifications may be made by those skilled in the art without departing from the spirit and scope of the invention and, therefore, the invention is not to be limited to the specific embodiments disclosed below.

In the description of the present invention, it should be noted that the terms "inside", "outside", "upper", "lower", "horizontal", and the like indicate the directions or positional relationships based on the directions or positional relationships shown in the drawings, or the directions or positional relationships that the products of the present invention are usually placed when used, and are only for the convenience of describing the present invention and simplifying the description, but not for indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," "fourth," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.

In the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," "secured," "attached," and the like are to be construed broadly, e.g., as being fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

As shown in fig. 1 to 5, in one embodiment, a sample processing apparatus is provided, which includes a machine body 100, and a liquid storage box 200, a reaction pot 300, and a liquid receiving tube 400 sequentially disposed in the machine body 100, wherein a buffer solution or a frozen solution is disposed in the liquid storage box 200, the liquid storage box 200 is communicated with the reaction pot 300 through a pipeline, an ultrasonic vibrator 310 with adjustable frequency is disposed on the reaction pot 300, and the liquid receiving tube 400 is connected with a liquid outlet of the reaction pot 300. Still including being used for placing the thing box 500 of putting of tissue sample, put the thing box 500 and place the holding intracavity at reaction kettle 300, put and be equipped with a plurality of through-holes 510 with the holding chamber intercommunication on the thing box 500.

In this embodiment, through connect the setting in proper order stock solution box 200, reaction pot 300 and connect liquid pipe 400 in an organism 100, utilize stock solution box 200 to deposit buffer solution or frozen stock solution, when carrying out the operation, carry buffer solution or frozen stock solution in the stock solution box 200 to the reaction pot 300 in, put into the thing box 500 of putting that is equipped with the tissue sample in the reaction pot 300, be equipped with a plurality of through-holes 510 that communicate with reaction pot 300 holding chamber on putting the thing box 500, so that buffer solution or frozen stock solution in the reaction pot 300 can flow into put the thing box 500 in, make the tissue sample soak in buffer solution or frozen stock solution. Then, the ultrasonic cavitation technology is utilized to break and peel off the superficial layer cells of the tissue sample layer by layer through the ultrasonic vibrator 310 arranged on the reaction kettle 300, the peeled cells flow into the reaction kettle 300 along with the buffer solution or the frozen stock solution through the through hole 510 of the storage box 500 and are discharged into the liquid receiving tube 400 from the liquid outlet, and the liquid receiving tube 400 collects the buffer solution or the frozen stock solution containing the cells and sends the buffer solution or the frozen stock solution to the next working procedure for operations such as separation, detection and the like.

Specifically, by using the ultrasonic cavitation technique, the frequency of the ultrasonic vibrator 310 and the time of ultrasonic treatment are controlled, so that the tissue sample can be completely crushed or the superficial cell layer can be crushed and peeled. The whole broken tissue can be directly prepared into cell suspension; after the superficial layer cells of the tissue sample are exfoliated, the exfoliated cells can be used for cell subsequent detection, and the remaining undamaged tissues can be used for pathological detection, so that the utilization rate of the tissue sample can be improved. Particularly, when the lesion site is small or the lesion site is not easily obtained, the simultaneous detection of the pathological biopsy and the cell typing (or immune state) cannot be realized. The technology can reduce the waste of tissue samples and the wound of patients caused by obtaining the tissue samples from the bodies of the patients.

In actual operation, still be equipped with lid 520 on putting thing box 500, the one end of lid 520 is connected through soft connecting portion 530 with the box body of putting thing box 500 to realize the fixed of lid 520 and box body, conveniently realize simultaneously that lid 520 seals and opens the box body. In addition, the bottom of the reaction kettle 300 is inclined downwards towards the liquid outlet, so that under the action of gravity, the liquid in the reaction kettle 300 can flow out in a concentrated manner towards the liquid outlet. Further, a filter screen can be arranged at the outlet of the reaction pot 300, so that a tissue sample of larger particles obtained by crushing is filtered, and the pressure of separation and detection in subsequent operation is reduced.

In one embodiment, the machine body 100 is provided with an operation platform 110, and the reaction pot 300 and the liquid receiving tube 400 are placed on the operation platform 110, so that an operator can conveniently place and take out the storage box 500 and the liquid receiving tube 400. Meanwhile, the operator can observe the real-time state of the ultrasonic cavitation treatment of the tissue sample and the condition that the broken cells flow into the liquid receiving tube 400 along with the buffer solution or the frozen stock solution conveniently and intuitively.

In one embodiment, the liquid receiving rack 120 is disposed on the operation platform 110, a placing cavity 121 for placing the liquid receiving tube 400 is disposed in the liquid receiving rack 120, and an opening gradually inclined from inside to outside is disposed at an upper end of the liquid receiving rack 120. Specifically, the size of the placement cavity 121 is slightly larger than the pipette 400 so that the pipette 400 is placed and kept fixed. Further, by arranging the opening at the upper end of the liquid receiving rack 120 to be gradually inclined from inside to outside, in actual operation, the opening is arranged to be a trumpet-shaped opening, so that the operator can place and take out the liquid receiving pipe 400. Of course, in other embodiments, other opening structures may be adopted, as long as the operation of placing and taking out the liquid receiving tube 400 by the operator is convenient, and such designs all belong to the protection scope of the present invention.

In one embodiment, the reaction kettle further comprises a waste liquid box 600, and the waste liquid box 600 is connected with the liquid outlet of the reaction kettle 300. After the tissue sample is subjected to ultrasonic cavitation and fragmentation, and a sufficient amount of liquid containing the tissue sample is collected through the liquid collecting tube 400, since residual liquid is remained in the reaction pot 300, buffer solution or frozen stock solution needs to be continuously conveyed into the reaction pot 300 through the liquid storage box 200, the residual liquid is discharged out of the reaction pot 300, and the reaction pot 300 is cleaned, so that the problem that the residual liquid in the reaction pot 300 is mixed with new liquid to cause errors in subsequent detection in the next operation is avoided.

Further, the liquid receiving rack 120 is disposed between the reaction kettle 300 and the waste liquid box 600, one end of the placing cavity 121 is connected to a liquid outlet of the reaction kettle 300, and a liquid outlet 122 communicated with the waste liquid box 600 is disposed at the bottom of the placing cavity 121. When the liquid receiving pipe 400 receives and takes out the liquid receiving pipe 400 after enough liquid is taken out, the buffer liquid or the frozen stock liquid in the liquid storage box 200 is controlled to continuously flow out at the moment, and the residual liquid in the reaction pot 300 is flushed out through the pipeline, and the residual liquid directly falls into the placing cavity 121 of the liquid receiving frame 120 from the pipeline because the liquid receiving pipe 400 is taken out, and is discharged into the waste liquid box 600 through the liquid outlet hole 122 at the bottom of the placing cavity 121, so that the waste liquid recovery is realized.

In one embodiment, the machine body 100 is further provided with a protective cover 130 for sealing the operation platform 110, one end of the protective cover 130 is hinged to the machine body 100, the operation platform 110 is sealed by the protective cover 130, and in the operation process, the operation platform 110 is ensured to be sealed in the whole process, so that dust is prevented from falling into the reaction pot 300, the liquid receiving tube 400 and other devices arranged on the operation platform 110 to cause sample pollution. Further, the protection cover 130 is made of transparent material, so as to ensure that the operation condition can be monitored by an operator in real time.

In one embodiment, the machine body 100 is provided with a storage box 140 for storing the storage boxes 200, the storage box 140 is provided with a sealing door 141, the sealing door 141 is hinged with the machine body 100 through a rotating shaft 142, and one or more storage boxes 200 are arranged. Make things convenient for storage and the placing of stock solution box 200 through storage tank 140 to can be favorable to avoiding carrying out the in-process that the device removed, stock solution box 200 receives the damage of colliding with and causes the condition of weeping. Meanwhile, the storage box 200 is sealed in the machine body 100 through the storage box 140 sealing door 141, which is also beneficial to avoiding the influence of external environment such as illumination, temperature and other factors on the buffer solution or the frozen stock solution stored in the storage box 200 to influence the quality of the buffer solution or the frozen stock solution.

In one embodiment, the device further comprises a plurality of driving mechanisms for driving the liquid flow among the liquid storage box 200, the reaction pot 300 and the liquid receiving tube 400, wherein the driving mechanisms are respectively arranged between the liquid storage box 200 and the reaction pot 300 and between the reaction pot 300 and the liquid receiving tube 400. Of course, in other embodiments, gravity may be used as the power for the liquid flow by adjusting the distribution positions of the liquid storage box 200, the reaction vessel 300 and the liquid receiving tube 400 on the machine body 100. Further, in the above embodiment, a driving mechanism is also provided between the waste liquid box 600 and the liquid receiving rack 120 to provide power for discharging the waste liquid. Of course, the waste liquid box 600 may also be disposed at the bottom of the liquid receiving frame 120, and the waste liquid is discharged by gravity.

Further, since less tissue sample is typically required, less fluid is required to extract the cells. In this embodiment, the driving mechanism is set as the peristaltic pump 700, so that the peristaltic pump 700 pumps liquid at a relatively low speed, which is beneficial to conveniently controlling the liquid outlet speed and avoiding overflow of the liquid due to too fast liquid outlet.

In one embodiment, a liquid outlet pipe 800 is disposed between the reaction kettle 300 and the liquid receiving pipe 400, a liquid outlet of the liquid outlet pipe 800 is disposed above the liquid receiving pipe 400, and the liquid outlet pipe 800 is telescopically disposed in the machine body 100. Specifically, the liquid outlet pipe 800 is partially retracted in the machine body 100 initially, and when the liquid outlet is performed normally, the liquid receiving pipe 400 is put in, and the liquid outlet pipe 800 is pulled out, so that the liquid outlet of the liquid outlet pipe 800 is located right above the liquid receiving pipe 400, and thus the liquid directly falls into the liquid receiving pipe 400 after flowing out from the liquid outlet. After the liquid is discharged, the liquid outlet pipe 800 is retracted into the machine body 100 to take out the liquid receiving pipe 400, and the liquid outlet pipe 800 is arranged in a telescopic manner to avoid obstructing the taking out of the liquid receiving pipe 400.

Specifically, in actual operation, a connecting plate 123 is disposed at one end of the liquid receiving frame 120, a sliding groove 124 is disposed on the connecting plate 123, and a sliding block 810 slidably connected to the sliding groove 124 is disposed on the liquid outlet pipe 800, so as to achieve sliding connection between the liquid outlet pipe 800 and the connecting plate 123, and achieve sliding and stretching of the liquid outlet pipe 800 on the machine body 100.

Certainly, in the utility model discloses in, do not restrict the installation connected mode with drain pipe 800, also can adopt the structural design who rotates drain pipe 800 and organism 100 to be connected, as long as can realize in leading-in liquid receiving pipe 400 with the liquid in the reaction kettle 300 to place and take out the operation and cause the hindrance can liquid receiving pipe 400, such a design all belongs to the protection scope of the utility model.

Further, in an embodiment, the operation platform 110 is further provided with a touch screen 150, and is electrically connected to the ultrasonic vibrator 310 and the peristaltic pump 700 through the touch screen 150, so that the operations of switching and frequency adjustment of the ultrasonic vibrator 310, switching and flow rate adjustment of the peristaltic pump 700 and the like can be directly controlled through the touch screen 150, and the operating states and parameters of the ultrasonic vibrator 310, the peristaltic pump 700 and the like can be displayed in real time through the touch screen 150, so that an operator can monitor in real time in the whole operation process, and the operation safety is ensured.

The above examples only represent some embodiments of the present invention, and the description thereof is more specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. The sample processing device is characterized by comprising a machine body (100), a liquid storage box (200), a reaction pot (300) and a liquid receiving pipe (400), wherein the liquid storage box (200), the reaction pot (300) and the liquid receiving pipe (400) are sequentially connected in the machine body (100), a buffer solution or a frozen solution is placed in the liquid storage box (200), the liquid storage box (200) is communicated with the reaction pot (300) through a pipeline, an ultrasonic vibrator (310) with adjustable frequency is arranged on the reaction pot (300), and the liquid receiving pipe (400) is connected with a liquid outlet of the reaction pot (300);

still including putting thing box (500) that is used for placing the tissue sample, it places to put thing box (500) the holding intracavity of reaction kettle (300), put be equipped with on thing box (500) a plurality of with through-hole (510) of holding chamber intercommunication.

2. The sample processing device according to claim 1, wherein the body (100) is provided with an operation platform (110), and the reaction pot (300) and the liquid receiving tube (400) are placed on the operation platform (110).

3. The sample processing device according to claim 2, wherein a liquid receiving rack (120) is disposed on the operation platform (110), a placing cavity (121) for placing the liquid receiving tube (400) is disposed in the liquid receiving rack (120), and an opening gradually inclined from inside to outside is disposed at an upper end of the liquid receiving rack (120).

4. The sample processing device according to claim 3, further comprising a waste liquid box (600), wherein the waste liquid box (600) is connected with the liquid outlet of the reaction vessel (300).

5. The sample processing device according to claim 4, wherein the liquid receiving rack (120) is disposed between the reaction pot (300) and the waste liquid box (600), one end of the placing cavity (121) is connected with a liquid outlet of the reaction pot (300), and a liquid outlet hole (122) communicated with the waste liquid box (600) is disposed at the bottom of the placing cavity (121).

6. The sample processing device according to claim 3, wherein the body (100) is further provided with a protective cover (130) for sealing the operation platform (110), and one end of the protective cover (130) is hinged on the body (100).

7. The sample processing device according to claim 1, wherein a storage box (140) for storing the liquid storage box (200) is arranged on the body (100), a closing door (141) is arranged on the storage box (140), the closing door (141) is hinged with the body (100) through a rotating shaft (142), and one or more liquid storage boxes (200) are arranged.

8. The sample processing device according to claim 1, further comprising a driving mechanism for driving liquid flow between the reservoir cartridge (200), the reaction pan (300) and the liquid receiving tube (400), the driving mechanism being respectively disposed between the reservoir cartridge (200) and the reaction pan (300) and between the reaction pan (300) and the liquid receiving tube (400).

9. The sample processing device according to claim 8, wherein the drive mechanism is provided as a peristaltic pump (700).

10. The sample processing device according to claim 1, wherein a liquid outlet pipe (800) is disposed between the reaction pot (300) and the liquid receiving pipe (400), a liquid outlet of the liquid outlet pipe (800) is disposed above the liquid receiving pipe (400), and the liquid outlet pipe (800) is telescopically disposed in the body (100).