CN218629829U - Creatinine detection card - Google Patents
Creatinine detection card Download PDFInfo
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- CN218629829U CN218629829U CN202221879431.6U CN202221879431U CN218629829U CN 218629829 U CN218629829 U CN 218629829U CN 202221879431 U CN202221879431 U CN 202221879431U CN 218629829 U CN218629829 U CN 218629829U
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Abstract
The utility model discloses a creatinine detects card relates to creatinine and examines technical field soon. The utility model discloses a creatinine detects card includes the sample gasket, and the membrane is handled to the blood specimen, and the combination gasket, nitrocellulose membrane, blood specimen detection line T1, urine detection line T2, quality control line C support the gasket, the pad that absorbs water, plastic housing, the sample addition mouth, the detection mouth, the bar code is regional, blood specimen detection line T1, urine creatinine detection line T2 contain the creatinine antigen, and quality control line C contains goat anti mouse antibody, makes the utility model discloses a creatinine detects card can be compatible to the short-term test of blood specimen creatinine and urine creatinine.
Description
Technical Field
The utility model relates to a creatinine examines technical field soon, specifically, relates to a creatinine detects card.
Background
Creatinine is a low molecular weight nitrogenous compound, and is a metabolic end product produced from creatine phosphate and creatine in muscle tissue through a nonenzymatic, irreversible dephosphorylation reaction. Creatinine is filtered only from the glomeruli into the urine and is not reabsorbed by the renal tubules. Serum creatinine concentration is an important indicator for evaluating glomerular filtration function.
At present, the methodologies for detecting creatinine mainly comprise a picric acid method, an enzyme method, a radionuclide dilution mass spectrometry method, a high performance liquid chromatography, a capillary electrophoresis method, an electrode method and the like. In the clinical use process, most products use a picric acid method and an enzyme method, and other methods are complex to operate and high in operation cost. However, the picric acid method is based on a simpler chemical reaction, has a great interference and a tendency of being gradually replaced, and the enzymatic detection of creatinine becomes the mainstream. However, almost all enzymatic reagents need to be refrigerated and stored, have short expiration date, need to recover temperature in advance in the using process, and cannot be well applicable to detection scenes needing to give reports in time
Clinical guidelines for renal injury often suggest that urinary creatinine be measured simultaneously with blood creatinine for use in calculating creatinine clearance to assess glomerular filtration function. However, the content of the creatinine in the blood of a normal human body is 44-133uM/L, while the content of the creatinine in the urine is about 8.4-13.2mM/L, which is more than one hundred times of that of a blood sample, and the existing quick test products cannot be compatible.
In the field of rapid detection reagents capable of rapidly obtaining detection results, products for detecting creatinine items do not exist at present, so that a rapid detection product capable of compatibly detecting creatinine in blood samples and creatinine in urine is urgently needed.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a creatinine detects card to solve the problem that the background art provided.
In order to achieve the above object, the utility model provides a creatinine detects card, including sample gasket (1), blood specimen processing membrane (2), combination thing gasket (3), nitrocellulose membrane (4), blood specimen detection line T1 (5), urine detection line T2 (6), quality control line C (7), support gasket (8), pad that absorbs water (9), plastic casing (10), sample addition port (11), detect mouthful (12) and bar code region (13).
The blood sample detection line T1 (5), the urine detection line T2 (6) and the quality control line C (7) are arranged on the nitrocellulose membrane (4).
The plastic shell (10) is provided with two openings, namely a sample adding opening (11) and a detection opening (12)
The detection port (12) is positioned above the blood sample detection line T1 (5), the urine creatinine detection line T2 (6) and the quality control line C (7).
The blood sample detection line T1 (5) is closest to the sample adding port, the urine detection line T2 (6) is arranged next, the quality control line C (7) is arranged last, and the sequence of the blood sample detection line T1 (5), the urine detection line T2 (6) and the quality control line C (7) is not adjustable.
The blood sample detection line T1 and the urine detection line T2 contain creatinine antigen, and the quality control line C contains goat anti-mouse antibody.
The sample gasket (1) is made of glass fiber materials, and plays a role in primary filtration of liquid samples.
The blood sample processing membrane (2) is made of glass fiber materials and plays a role in intercepting red blood cells in a blood sample.
The conjugate pad (3) is made of polyester fiber and plays a role of a carrier of an antibody conjugate.
The nitrocellulose membrane (4) is made of nitrocellulose, plays a role of combining an antigen and an antibody with a carrier, and is also a signal detection area.
The support gasket (8) is made of PCV materials and plays a role in supporting.
The water absorption pad (9) is made of plant fiber materials and plays a role in absorbing liquid and promoting liquid chromatography.
The bar code area (13) is formed by printing code on the plastic shell without an opening.
The content of creatinine antigens coated by a blood sample detection line T1 (5) and a urine detection line T2 (6) of the nitrocellulose membrane (4) is different, the concentration of the antigens matched with the blood sample detection line T1 is 0.1mg/ml, the concentration of the antigens matched with the urine creatinine detection line T2 is 2mg/ml, and the concentration of the antibodies matched with a quality control line C (7) is 1mg/ml.
The blood sample detection line T1 (5), the urine detection line T2 (6) and the quality control line C (7) are equal in distance, and the distance is 5-10 mm.
The utility model discloses a creatinine detects card application method carries out according to following step:
dropping 800uL diluted sample into the sample addition port, collecting fluorescence signals by using an immunoassay analyzer after 5-10 minutes, and respectively calculating detection signals of different samples by adopting T1/C and (T1 + T2)/C to obtain the detection value of the creatinine sample.
The sample is a blood sample or a urine sample, and the type of the sample can be selected after the barcode region (13) is identified by software.
The utility model provides a technical scheme has following beneficial effect at least:
(1) The compatibility of the detection card for detecting creatinine blood samples and urine samples is realized through the arrangement of the blood sample detection line T1 and the urine detection line T2.
(2) The sequence of a blood sample detection line T1, a urine detection line T2 and a quality control line C is specified, and T1/C and (T1 + T2)/C are adopted to calculate detection signals of different samples respectively, so that the detection of the blood sample and the urine sample can obtain better accuracy.
(3) The detection is carried out by antigen-antibody reaction based on solid-phase chromatography, the detection specificity is good, the sample is stored at normal temperature, the creatinine blood sample and the urine sample can be detected at any time, the field operation is simplified, and the kit is suitable for the detection scene needing to give a report in time.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural view of the creatinine detection card of the present invention;
in the figure: the kit comprises a sample gasket (1), a blood sample processing membrane (2), a conjugate gasket (3), a nitrocellulose membrane (4), a blood sample detection line T1 (5), a urine detection line T2 (6), a quality control line C (7), a support gasket (8), a water absorption pad (9), a plastic shell (10), a sample adding port (11), a detection port (12) and a barcode region (13).
Detailed Description
In order to facilitate understanding of the present invention, the technical solution of the present invention will be described more fully and meticulously with reference to the drawings and preferred embodiments, but the scope of protection of the present invention is not limited to the following specific embodiments, and all other embodiments obtained by a person skilled in the art without creative work belong to the scope of protection of the present invention based on the embodiments of the present invention.
It will be understood that when an element is referred to as being "attached" to, secured to, connected to or communicating with another element, it can be directly attached to, secured to, connected to or communicating with the other element or indirectly attached to, secured to, connected to or communicating with the other element through other intervening elements.
Unless otherwise defined, all terms of art used hereinafter have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention.
Example 1
A rapid detection card capable of detecting creatinine and urine creatinine of a blood sample simultaneously comprises a shell and detection test paper arranged in the shell.
The detection card is used for detecting creatinine in blood samples and creatinine in urine. The detection card comprises a sample pad (1), a blood sample processing membrane (2), a conjugate pad (3), a nitrocellulose membrane (4), a blood sample detection line T1 (5), a urine detection line T2 (6), a quality control line C (7), a support pad (8), a water absorption pad (9), a plastic shell (10), a sample adding port (11), a detection port (12) and a bar code area (13).
And (3) dripping 80uL of diluted sample into the sample addition port, collecting a fluorescence signal by using an immunoassay analyzer after 10 minutes, and respectively calculating detection signals of different samples by adopting T1/C and (T1 + T2)/C to obtain the detection value of the creatinine sample. The sample is a blood sample or a urine sample.
Example 2
The utility model provides a can detect quick test card of blood sample creatinine and urine creatinine simultaneously, includes the shell and sets up the test paper in the shell.
The test paper is used for detecting creatinine of a blood sample and urine creatinine. The detection card comprises a sample pad (1), a blood sample processing membrane (2), a conjugate pad (3), a nitrocellulose membrane (4), a blood sample detection line T1 (5), a urine detection line T2 (6), a quality control line C (7), a support pad (8), a water absorption pad (9), a plastic shell (10), a sample adding port (11), a detection port (12) and a bar code area (13).
The preparation method of the detection test paper comprises the following steps:
1) Taking 1mg of fluorescent microspheres with proper particle size, adding NHS and EDC for activation, coupling the activated microspheres with 0.1mg of creatinine antibody, incubating for more than 4 hours, using 0.5% BSA solution for sealing for 1 hour, centrifuging to remove supernatant, suspending the obtained microspheres in buffer solution, spraying the buffer solution on a pretreated conjugate pad, and drying;
2) 10mg/ml BSA solution and 2mg/ml creatinine solution were mixed in a 1:5, and mixing. Adding 100ul of 10mg/ml EDC solution, reacting at normal temperature for 2 hours, and performing centrifugal purification to obtain creatinine antigen;
3) Preparing PBS buffer solution containing 2% sucrose, adding creatinine antigen to prepare two concentrations of 0.1mg/ml and 2mg/ml, respectively spraying the concentrations of 0.1mg/ml and 2mg/ml on nitrocellulose membrane to form two detection lines, and spraying C line antibody with the concentration of 1mg/ml as a control strip, wherein the interval between the three lines is 5mm;
4) Pretreating the sample pad and the blood sample treatment membrane by using a pretreatment buffer solution, taking out and drying at 37 ℃ overnight;
5) And connecting the sample gasket, the blood sample treatment film, the conjugate gasket, the nitrocellulose membrane and the water absorption gasket in sequence, adhering the sample gasket, the blood sample treatment film, the conjugate gasket, the nitrocellulose membrane and the water absorption gasket to a support gasket to obtain an assembled large card, cutting the large card according to the width of 4cm, assembling the cut card into a code-sprayed plastic card shell, and sealing and storing the card to obtain the creatinine detection card.
The above description is only a partial example of the present invention, and does not limit the scope of the present invention, and it will be apparent to those skilled in the art that various modifications and variations can be made in the present invention. Any improvement or equivalent replacement made by utilizing the contents of the specification and the drawings of the present invention can be directly or indirectly applied to other related technical fields within the spirit and principle of the present invention, and shall be included in the scope of the present invention.
Claims (3)
1. A creatinine detection card is characterized by comprising a sample pad (1), a blood sample processing membrane (2), a conjugate pad (3), a nitrocellulose membrane (4), a blood sample detection line T1 (5), a urine detection line T2 (6), a quality control line C (7), a support pad (8), a water absorption pad (9), a plastic shell (10), a sample adding port (11), a detection port (12) and a bar code area (13);
the blood sample detection line T1 (5), the urine detection line T2 (6) and the quality control line C (7) are arranged on the nitrocellulose membrane (4);
the plastic shell (10) is provided with two openings, namely a sample adding opening (11) and a detection opening (12);
the detection port (12) is positioned above the blood sample detection line T1 (5), the urine detection line T2 (6) and the quality control line C (7);
the blood sample detection line T1 (5) is closest to the sample adding port, the urine detection line T2 (6) is arranged next, the quality control line C (7) is arranged last, and the sequence of the blood sample detection line T1 (5), the urine creatinine detection line T2 (6) and the quality control line C (7) is not adjustable;
the blood sample detection line T1 and the urine detection line T2 contain creatinine antigen, and the quality control line C contains goat anti-mouse antibody.
2. The creatinine test card according to claim 1, wherein the blood sample test line T1 (5) is matched to an antigen concentration of 0.1mg/ml, the urine test line T2 (6) is matched to an antigen concentration of 2mg/ml, and the quality control line C (7) is matched to an antibody concentration of 1mg/ml.
3. The creatinine detection card according to claim 1, wherein the blood sample detection line T1 (5), the urine detection line T2 (6), and the quality control line C (7) are equally spaced at intervals of 5 to 10mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221879431.6U CN218629829U (en) | 2022-07-20 | 2022-07-20 | Creatinine detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221879431.6U CN218629829U (en) | 2022-07-20 | 2022-07-20 | Creatinine detection card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218629829U true CN218629829U (en) | 2023-03-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221879431.6U Active CN218629829U (en) | 2022-07-20 | 2022-07-20 | Creatinine detection card |
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| Country | Link |
|---|---|
| CN (1) | CN218629829U (en) |
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2022
- 2022-07-20 CN CN202221879431.6U patent/CN218629829U/en active Active
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