CN218627509U

CN218627509U - Freeze-drying device suitable for conical bottom storage tube

Info

Publication number: CN218627509U
Application number: CN202222845190.XU
Authority: CN
Inventors: 方剑秋; 沙海天; 夏龚杰; 白艳军
Original assignee: Shanghai Wanzijian Biotechnology Co ltd
Current assignee: Shanghai Wanzijian Biotechnology Co ltd
Priority date: 2022-10-27
Filing date: 2022-10-27
Publication date: 2023-03-14
Anticipated expiration: 2032-10-27

Abstract

The utility model relates to a freeze-drying technical field especially relates to a freeze-drying device suitable for cone end storage tube, cone end storage tube includes the cylindricality section and the back taper section that is connected with the bottom of cylindricality section, freeze-drying device includes the freeze-drying tray, upwards extend on the upper surface of freeze-drying tray and be formed with a plurality of fixed column, the equal undercut of upper surface of every fixed column forms the jack, the jack includes the cylindricality through-hole and the back taper hole groove that is linked together with the lower extreme of cylindricality through-hole, the interior profile of cylindricality through-hole and the outline phase-match of the bottom of cylindricality section, the interior profile of back taper hole groove and the outline phase-match of back taper section. The cone bottom storage tube can be completely attached to the inner contour surface of the jack when the inverted cone section and the cylindrical section of the cone bottom storage tube are inserted into the jack, so that the cone bottom storage tube is fully contacted with a freeze-drying tray (liquid cooling plate), heat conduction and heat radiation of the cone bottom storage tube in the freeze-drying process are promoted, and the freeze-dried sample is complete in form, compact in structure and free of reduction in activity.

Description

Freeze-drying device suitable for conical bottom storage tube

Technical Field

The utility model relates to a freeze-drying technical field especially relates to a freeze-drying device suitable for awl end storage tube.

Background

Vacuum freeze-drying, which originated in the 20's of the 19 th century, was referred to as lyophilization or freeze-drying, a dehydration process that is commonly used to preserve perishable materials or to make materials more transportable.

The basic principle of freeze-drying is based on the tri-state change of water. The water has solid state, liquid state and gaseous state, and the three states can be mutually converted and coexist. When water is at the triple point (temperature of 0.01 deg.C, water vapor pressure of 610.5 Pa), water, ice and water vapor can coexist and balance each other. Under the high vacuum state, the sublimation principle is utilized, so that the moisture in the pre-frozen material is directly sublimated into water vapor in the ice state without melting the ice, and the water vapor is removed, thereby achieving the aim of freeze drying. The freeze-dried product is spongy, has no drying shrinkage, has excellent rehydration property and extremely low water content, and can be preserved and transported for a long time at normal temperature after being correspondingly packaged. Vacuum freeze-drying has become increasingly popular due to its incomparable advantages over other drying methods, and has become widely used in medicine, biological products and food. Most of biological products such as serum, strains, chinese and western medicines and the like are bioactive substances, and the vacuum freeze drying technology also provides a good solution for preserving the bioactivity.

In the 21 st century, vacuum freeze-drying technology has been widely used in fields other than medicine, biological products, food, blood products, and active substances.

With the development of in vitro diagnostic techniques, lyophilization technology has also been applied to the active storage and transport of in vitro diagnostic reagents. In molecular diagnosis, raw materials such as DNA amplification enzyme, dNTP, oligonucleotide, plasmid, cell and the like have extremely high requirements on the storage temperature, and the transportation cost of finished reagents is increased. Uncertainty also occurs during transportation, resulting in inactivation or reduction of activity of the active ingredient in the molecular diagnostic agent. The in vitro diagnostic reagent treated by the freeze-drying technology has no influence on the detection activity and reduces the dependence on the transportation condition. This will facilitate the transportation and storage of molecular diagnostic reagents and is of great significance to the development of extra-molecular diagnostic reagents.

At present, the freeze-drying equipment is mainly divided into a one-step method and a two-step method, wherein the one-step method adopts a cold trap and a freeze-drying area to be combined, and the two-step method separates the cold trap and the freeze-drying area. The freeze-drying area has the main function of freeze-drying the sample, and the cold trap has the main function of cooling. The freeze-drying district generally adopts liquid cooling board, evacuation to carry out the cooling drying to freeze-drying sample, and the heat-conduction and the heat radiation of freeze-drying district internal environment and sample container after the bottom of sample container and the laminating of liquid cooling board are relied on in the cooling of sample. The material and shape of the sample container also determine the shape and stability of the freeze-dried sample.

At present, a freeze-drying sample container commonly used is a penicillin bottle, and the penicillin bottle is generally made of glass materials and has a bottle bottom and good bottom heat conduction and bottle peripheral heat radiation effects. The molecular diagnostic reagent usually adopts a cone-bottom storage tube, the bottom of the cone-bottom storage tube is in an inverted cone shape, and the contact surface between the tube bottom and the liquid cooling plate is very small, so that the bottle bottom cannot be fully contacted with the liquid cooling plate like a penicillin bottle. This results in a low heat transfer efficiency between the conical bottom storage tube and the liquid-cooled plate, and the freeze-drying and shaping of the sample in the tube mainly depends on heat radiation. However, thermal radiation has great instability, and the mere dependence on thermal radiation to freeze-dry a sample in a sample container leads to poor stability after freeze-drying, large batch difference and the like. Therefore, a set of freeze-drying device specially used for freeze-drying the conical bottom storage tube is needed.

Among the prior art, publication No. CN 216970362U's utility model discloses a freeze-drying tray, it includes horizontal roof and annular lateral wall, thereby horizontal roof sets up the vacuole formation body on the annular lateral wall between horizontal roof and annular lateral wall, and horizontal roof is provided with vertical jack, and the vertical jack intercommunication cavity body, the mutual horizontal interval of a plurality of vertical jacks sets up, can place the PCR body upright, is convenient for carry out the freeze-drying to the PCR reactant in the PCR body.

The utility model patent publication No. CN211782299U discloses a freeze-drying tray, which comprises a tray body and a cover body; the tray body is provided with a bottom plate, a partition plate and two vertical plates which are oppositely arranged, the bottoms of opposite surfaces of the two vertical plates are respectively provided with a plug board groove, and the top surfaces of the vertical plates are provided with sliding grooves; the two opposite sides of the bottom plate are inserted in the two inserting plate grooves in a sliding manner, the partition plate is arranged between the two vertical plates and positioned above the bottom plate, and a plurality of through holes are formed in the partition plate and are suitable for separating a plurality of freeze-drying bottles; the bottom surface of the cover body is provided with a sliding plate which is suitable for being inserted in the sliding groove in a sliding way so as to cover the cover body above the bottle mouth of the freeze-drying bottle. The bottom plate is pegged graft when the picture peg groove, and the through-hole on the freeze-drying bottle accessible division board is placed on the bottom plate for tray body transports freeze-drying bottle to freeze-drying machine in batches, when in the freeze-drying machine, can take out the bottom plate, when the bottom plate was taken out from the picture peg inslot, the shelf of the bottle bottom contact freeze-drying machine of freeze-drying bottle, the bottleneck terminal surface of freeze-drying bottle is in on the same horizontal plane with the top surface of riser.

The utility model patent of publication No. CN211261717U discloses a freeze-drying tray with adjustable depth, which comprises a tray fixing frame, wherein the tray fixing frame is of a rectangular frame structure, a tray core is fixedly arranged in the tray fixing frame, the outer side wall of the tray core is tightly attached to the inner side wall of the tray fixing frame, the top end of the tray core is uniformly provided with a carrying groove, the carrying groove is of a cylindrical cavity structure, the number of the carrying grooves is a plurality, cover bodies are arranged above the carrying grooves, and carrying cylinders are embedded in the carrying grooves; the tray core is fixed through inserted bar and constant head tank, and is consequently dismantled and assembled, when big object or irregular object are placed to needs, then pull down the tray core, when the object that needs to place is bottle or rectangular form, can load onto the tray core, utilizes the objective lens on the tray core to come the splendid attire material.

The three representative freeze-drying trays have corresponding use scenes and functions, but cannot meet the freeze-drying requirement of the conical-bottom storage tube.

In addition, after the freeze-drying process of the freeze-dried sample is completed, in order to prevent the freeze-dried sample from being affected with moisture, shrinkage, morphological change and change of the activity of the sample, it is preferable to perform a gland sealing in a vacuum environment of the freeze-drying region after the freeze-drying process is completed, so that the water content in the freeze-drying container can be reduced as much as possible, and the freeze-dried sample can be prevented from being affected with moisture, shrinkage, morphological change and change of the activity of the sample due to the entry of water. For sample freeze-drying with a freeze-drying container being a penicillin bottle, before a freeze-drying procedure is executed, a rubber soft plug with a vent groove is placed on the penicillin bottle; after the freeze-drying procedure is completed, the stopper is pressed in the vacuum environment of the freeze-drying area of the freeze dryer, and the freeze-dried sample is taken out after the stopper is pressed. Therefore, when penicillin bottles are used as freeze-drying containers, the problem of vacuum gland sealing is avoided, and the conical-bottom storage tube for molecular diagnostic reagents is usually an external-screwing type threaded cap, so that the cap cannot be pressed.

SUMMERY OF THE UTILITY MODEL

In view of the above-mentioned defects in the prior art, the to-be-solved technical problem of the present invention is to provide a freeze-drying device suitable for conical bottom storage tubes, which can make the conical bottom storage tubes achieve better heat conduction during freeze-drying.

In order to solve the technical problem, the utility model discloses a following technical scheme:

the utility model provides a freeze-drying device suitable for cone end storage tube, cone end storage tube includes the cylindricality section and the back taper section that is connected with the bottom of cylindricality section, freeze-drying device includes the freeze-drying tray, upwards extend on the upper surface of freeze-drying tray and be formed with a plurality of fixed column, the equal undercut of upper surface of every fixed column forms the jack, the jack includes cylindricality through-hole and the back taper hole groove that is linked together with the lower extreme of cylindricality through-hole, the outline phase-match of the bottom of the interior outline of cylindricality through-hole and cylindricality section, the outline phase-match of interior outline and back taper section of back taper hole groove.

Preferably, a portion of the inverted cone-shaped hole groove is located in the fixing column and another portion is located in the freeze-drying tray.

Preferably, the plurality of fixation posts are distributed in an array on the upper surface of the lyophilization tray.

Preferably, the freeze-drying device further comprises a tray handle, and a handle fixing hole for mounting the tray handle is formed in the upper surface of the freeze-drying tray.

Preferably, the tray handles are two, two sets of handle fixing holes are respectively formed in the edges of two opposite sides of the upper surface of the freeze-drying tray, and the two sets of handle fixing holes are respectively used for installing the two tray handles.

Preferably, the freeze-drying device further comprises an inner plug of the storage tube, the inner plug of the storage tube comprises a cylinder body and a top cover, the cylinder body is vertically communicated with the inner portion of the inner plug of the storage tube, the top cover is used for sealing the upper end of the cylinder body, the outer diameter of the cylinder body is consistent with the inner diameter of the top opening of the storage tube with the conical bottom, the outer periphery of the top cover protrudes out of the outer periphery of the cylinder body, the diameter of the top cover is consistent with the outer diameter of the top end of the storage tube with the conical bottom, and a vent groove is formed in the cylinder body; when the bottom of the column body is placed into the top opening of the conical-bottom storage tube, the inner space of the conical-bottom storage tube is communicated with the outer space through the vent groove; when the column body is pressed into the cone bottom storage tube, the top cover is attached to the top end face of the cone bottom storage tube, and the inner space of the cone bottom storage tube is not communicated with the outer space.

Preferably, one or more annular sealing rings are arranged on the outer peripheral surface of the cylinder along the circumferential direction.

Preferably, the at least one annular sealing ring is located above the vent channel.

Preferably, two vent grooves are formed in the column body, and the two vent grooves are arranged oppositely along the radial direction of the column body.

Compared with the prior art, the utility model discloses the progress that has showing:

the utility model discloses a freeze-drying device suitable for cone end storage tube, the confession cone end storage tube male jack that forms on freeze-drying tray has the outline assorted cylindricality through-hole of the bottom outline of interior profile and cone end storage tube cylindricality section and the outer profile assorted back taper hole groove of interior profile and cone end storage tube back taper section, can laminate with the interior profile face of jack completely when making the back taper section of cone end storage tube and the bottom part of cylindricality section insert in the jack, thereby realize cone end storage tube and freeze-drying tray (liquid cooling board) abundant contact, promote the heat conduction, the heat radiation of cone end storage tube in freeze-drying process, make the sample form after the freeze-drying complete, compact structure, activity does not reduce; the cylindrical through hole of the jack can ensure the heat conduction of the conical bottom storage pipe column section part and can also play a role in fixing and retaining the inserted conical bottom storage pipe.

Drawings

Fig. 1 is a schematic structural view of a cone-bottom storage tube in the embodiment of the present invention.

Fig. 2 is a schematic structural diagram of a freeze-drying tray in the freeze-drying device suitable for the cone-bottom storage tube according to the embodiment of the present invention.

Fig. 3 is a schematic top view of a freeze-drying tray in a freeze-drying device suitable for a conical-bottom storage tube according to an embodiment of the present invention.

Fig. 4 is a schematic front view of a freeze-drying tray in the freeze-drying device suitable for the cone-bottom storage tube according to the embodiment of the present invention.

Fig. 5 is a schematic front perspective view of a freeze-drying tray in the freeze-drying device suitable for the cone-bottom storage tube according to the embodiment of the present invention.

Fig. 6 is a schematic structural diagram of an inner plug of a storage tube in the freeze-drying device suitable for a conical-bottom storage tube according to an embodiment of the present invention.

Fig. 7 is a schematic front view of an inner plug of a storage tube in the freeze-drying device suitable for the conical-bottom storage tube according to the embodiment of the present invention.

Fig. 8 isbase:Sub>A schematic sectional view taken alongbase:Sub>A-base:Sub>A in fig. 7.

Wherein the reference numerals are as follows:

1. conical bottom storage tube

11. Cylindrical section

12. Inverted cone shaped section

2. Freeze-drying tray

21. Fixing column

22. Jack hole

221. Cylindrical through hole

222. Inverted cone-shaped hole groove

23. Handle fixing hole

3. Inner plug of preservation tube

31. Column body

32. Top cover

33. Ventilation trough

34. Ring-shaped sealing ring

Detailed Description

The following describes the present invention in further detail with reference to the accompanying drawings. These embodiments are provided only for illustrating the present invention and are not intended to limit the present invention.

In the description of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In addition, in the description of the present invention, "a plurality" means two or more unless otherwise specified.

As shown in fig. 1 to 8, the present invention provides an embodiment of a freeze-drying device suitable for a conical bottom storage tube.

Referring to fig. 1, the conical-bottom storage tube 1 includes a cylindrical section 11 and an inverted conical section 12 connected to the bottom end of the cylindrical section 11, and the conical-bottom storage tube 1 is a freeze-dried sample container for molecular diagnostic reagents.

Referring to fig. 2 to 5, the freeze-drying device suitable for the conical-bottom storage tube of the present embodiment includes a freeze-drying tray 2, a plurality of fixing posts 21 are formed on the upper surface of the freeze-drying tray 2 in an upward extending manner, the upper surface of each fixing post 21 is recessed downward to form a receptacle 22, and the receptacles 22 are isolated and independent from each other by the fixing posts 21. The insertion hole 22 is used for inserting the cone-bottom storage tube 1 and placing the cone-bottom storage tube on the freeze-drying tray 2 to perform the freeze-drying process, and the freeze-drying tray 2 is used as a liquid cooling plate. The plug hole 22 comprises a cylindrical through hole 221 and an inverted cone-shaped hole slot 222 communicated with the lower end of the cylindrical through hole 221, the inner contour of the cylindrical through hole 221 is matched with the outer contour of the bottom of the cylindrical section 11 of the cone bottom storage tube 1, namely, the inner contour shape and the size of the cylindrical through hole 221 are consistent with the outer contour shape and the size of the bottom of the cylindrical section 11 of the cone bottom storage tube 1, and the inner contour of the inverted cone-shaped hole slot 222 is matched with the outer contour of the inverted cone-shaped section 12 of the cone bottom storage tube 1, namely, the inner contour shape and the size of the inverted cone-shaped hole slot 222 are consistent with the outer contour shape and the size of the inverted cone-shaped section 12 of the cone bottom storage tube 1. Therefore, the bottom parts of the inverted cone-shaped section 12 and the cylindrical section 11 of the cone-bottom storage tube 1 can be completely attached to the inner contour surface of the insertion hole 22 when inserted into the insertion hole 22, so that the cone-bottom storage tube 1 is fully contacted with the freeze-drying tray 2 (liquid cooling plate), heat conduction and heat radiation of the cone-bottom storage tube 1 in the freeze-drying process are promoted, and a freeze-dried sample is complete in shape, compact in structure and free from reduction in activity; the cylindrical through hole 221 of the insertion hole 22 not only ensures the heat conduction of the cylindrical section 11 of the cone-bottom holding tube 1, but also plays a role in fixing and holding the inserted cone-bottom holding tube 1.

Referring to fig. 2 and 3, in the present embodiment, preferably, a plurality of fixing columns 21 are arranged in an array on the upper surface of the lyophilization tray 2.

Referring to fig. 5, in the present embodiment, preferably, a portion of the reverse tapered hole grooves 222 of the insertion hole 22 is located in the fixing post 21 and another portion is located in the lyophilization tray 2, whereby the cylindrical through hole 221 of the insertion hole 22 is located at the upper end portion of the fixing post 21, and the reverse tapered hole grooves 222 of the insertion hole 22 extend from the lower end portion of the fixing post 21 to below the upper surface of the lyophilization tray 2, i.e., to the inside of the lyophilization tray 2. Therefore, the thickness of the freeze-drying tray 2 is reduced, and the freeze-drying tray 2 can stably support the inserted conical-bottom storage tube.

Preferably, the freeze-drying device suitable for the conical-bottom storage tube of the present embodiment further includes a tray handle (not shown in the figure), referring to fig. 3, a handle fixing hole 23 for installing the tray handle is formed on the upper surface of the freeze-drying tray 2, and the tray handle is detachably connected to the handle fixing hole 23. When the tray handle is installed in the handle fixing hole 23 of the freeze-drying tray 2, the freeze-drying tray 2 can be grabbed and transferred through the tray handle.

Preferably, there are two tray handles, and two sets of handle fixing holes 23 are respectively formed at two opposite side edges of the upper surface of the freeze-drying tray 2, and the two sets of handle fixing holes 23 are respectively used for installing the two tray handles.

Referring to fig. 6 to 8, the freeze-drying device for conical-bottom storage tubes of the present embodiment preferably further comprises a storage tube inner plug 3, and the storage tube inner plug 3 is used for sealing the top opening of the conical-bottom storage tube 1. The inner plug 3 of the storage tube comprises a column 31 with the inside penetrating up and down and a top cover 32 for sealing the upper end of the column 31, the outer diameter of the column 31 is consistent with the inner diameter of the top opening of the conical-bottom storage tube 1, the outer peripheral edge of the top cover 32 protrudes out of the outer peripheral surface of the column 31, and the diameter of the top cover 32 is consistent with the outer diameter of the top end of the conical-bottom storage tube 1. The column 31 is provided with a vent groove 33. Preferably, the column 31 is provided with two ventilation slots 33, and the two ventilation slots 33 are oppositely arranged along the radial direction of the column 31. When the bottom of the cylinder 31 is placed into the top opening of the conical-bottom storage tube 1, the inner space of the conical-bottom storage tube 1 is communicated with the outer space through the vent groove 33, and air in the conical-bottom storage tube 1 is discharged in a vacuum state during freeze-drying. When the column 31 is pressed into the conical-bottom storage tube 1, i.e. after the cover is pressed, the top cover 32 is attached to the top end surface of the conical-bottom storage tube 1, the internal space of the conical-bottom storage tube 1 is not communicated with the external space, and the conical-bottom storage tube 1 is sealed. Preferably, one or more annular sealing rings 34 are provided on the outer circumferential surface of the cylinder 31 along the circumferential direction, and the annular sealing rings 34 are used for enhancing and ensuring the sealing performance of the conical-bottom preservation tube 1 after freeze-drying capping. Preferably, at least one annular sealing ring 34 is located above the vent groove 33. From this, the freeze-drying device who is applicable to awl end storage tube of this embodiment has solved the unable gland's of awl end storage tube problem among the prior art through keeping intraductal stopper 3, can realize accomplishing the gland under vacuum environment.

When the freeze-drying device is used, the conical-bottom storage tube 1 filled with a sample is placed in the jack 22 on the freeze-drying tray 2, the storage tube inner plug 3 is placed at the top opening of the conical-bottom storage tube 1 before freeze-drying, and the vent groove 33 is kept smooth, so that air in the conical-bottom storage tube 1 can be discharged through the vent groove 33 in the freeze-drying process. After the freeze-drying process is completed, the bottle is directly pressed in a vacuum environment in a freeze-drying area of a freeze dryer, so that the cylinder 31 of the inner plug 3 of the storage tube is completely pressed into the conical-bottom storage tube 1, the top cover 32 of the inner plug 3 of the storage tube is attached to the top end face of the conical-bottom storage tube 1 outside the conical-bottom storage tube 1, at the moment, the vent groove 33 is also completely pressed into the conical-bottom storage tube 1, air inside and outside the conical-bottom storage tube 1 is not circulated, and the conical-bottom storage tube 1 is sealed. After the capping is finished, the conical bottom storage tube 1 can be taken out, a sealed vacuum environment is arranged in the conical bottom storage tube 1, the water content in the conical bottom storage tube 1 can be reduced to the maximum extent, and the sample in the conical bottom storage tube 1 is kept dry, so that the sample is not easy to damp, shrink, change in form and change in activity of the sample during storage.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and replacements can be made without departing from the technical principle of the present invention, and these modifications and replacements should also be regarded as the protection scope of the present invention.

Claims

1. A freeze-drying device suitable for a cone-bottom storage tube, wherein the cone-bottom storage tube (1) comprises a cylindrical section (11) and an inverted cone-shaped section (12) connected with the bottom end of the cylindrical section (11), the freeze-drying device is characterized by comprising a freeze-drying tray (2), a plurality of fixing columns (21) extend upwards from the upper surface of the freeze-drying tray (2), the upper surface of each fixing column (21) is downwards sunken to form a jack (22), each jack (22) comprises a cylindrical through hole (221) and an inverted cone-shaped hole groove (222) communicated with the lower end of the cylindrical through hole (221), the inner contour of the cylindrical through hole (221) is matched with the outer contour of the bottom of the cylindrical section (11), and the inner contour of the inverted cone-shaped hole groove (222) is matched with the outer contour of the inverted cone-shaped section (12).

2. The lyophilization apparatus for cone-bottom storage tubes according to claim 1, wherein a portion of the inverted cone-shaped hole groove (222) is located in the fixing column (21) and another portion is located in the lyophilization tray (2).

3. The lyophilization apparatus for conical-bottomed storage tubes according to claim 1, wherein a plurality of the fixing columns (21) are distributed in an array on the upper surface of the lyophilization tray (2).

4. The freeze-drying device suitable for the cone-bottom storage tube is characterized by further comprising a tray handle, wherein a handle fixing hole (23) for mounting the tray handle is formed in the upper surface of the freeze-drying tray (2).

5. The freeze-drying device for the conical-bottom storage tube is characterized in that the number of the tray handles is two, two sets of the handle fixing holes (23) are respectively formed in two opposite side edges of the upper surface of the freeze-drying tray (2), and the two sets of the handle fixing holes (23) are respectively used for installing the two tray handles.

6. The freeze-drying device suitable for the conical-bottom storage tube is characterized by further comprising a storage tube inner plug (3), wherein the storage tube inner plug (3) comprises a cylinder (31) which is internally communicated up and down and a top cover (32) which closes the upper end of the cylinder (31), the outer diameter of the cylinder (31) is consistent with the inner diameter of the top opening of the conical-bottom storage tube (1), the outer periphery of the top cover (32) protrudes out of the outer periphery of the cylinder (31), the diameter of the top cover (32) is consistent with the outer diameter of the top end of the conical-bottom storage tube (1), and a vent groove (33) is formed in the cylinder (31); when the bottom of the cylinder (31) is placed into the top opening of the conical-bottom storage tube (1), the inner space of the conical-bottom storage tube (1) is communicated with the outer space through the vent groove (33); when the cylinder (31) is pressed into the conical bottom storage tube (1), the top cover (32) is attached to the top end face of the conical bottom storage tube (1), and the inner space of the conical bottom storage tube (1) is not communicated with the outer space.

7. The lyophilization apparatus for cone-bottom storage tubes according to claim 6, wherein the cylindrical body (31) is provided with one or more annular sealing rings (34) along the circumferential direction on the outer circumferential surface.

8. Freeze-drying device for tapered-bottom holding tubes according to claim 7, characterized in that at least one of said annular sealing rings (34) is located above said vent groove (33).

9. The lyophilization apparatus for cone-bottom storage tubes according to claim 6, wherein the cylinder (31) is provided with two vent grooves (33), and the two vent grooves (33) are arranged oppositely along the radial direction of the cylinder (31).