CN218620761U - Anaerobic bacteria culture medium deaerating plant of area partial shipment function - Google Patents
Anaerobic bacteria culture medium deaerating plant of area partial shipment function Download PDFInfo
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- CN218620761U CN218620761U CN202221602828.0U CN202221602828U CN218620761U CN 218620761 U CN218620761 U CN 218620761U CN 202221602828 U CN202221602828 U CN 202221602828U CN 218620761 U CN218620761 U CN 218620761U
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Abstract
The utility model provides an anaerobic culture medium deoxidizing device with a split charging function, wherein the mouth of a glass bottle is sealed by a rubber plug, and the bottle is filled with a culture medium; the exhaust non-return device is used for receiving oxygen discharged from the glass bottle, an exhaust port is reserved, and liquid paraffin is filled in the exhaust non-return device; the anaerobic container is used for receiving the culture medium after oxygen removal; the first vent pipe is inserted into the culture medium and used for introducing deoxygenated gas into the glass bottle, the vent pipe is communicated between the glass bottle and the exhaust check device, a pipe orifice at one end is flush with the bottom end of the rubber plug, and a pipe orifice at the other end is vertically inserted into the liquid paraffin; the liquid separating pipe is communicated between the glass bottle and the anaerobic container, one end of the liquid separating pipe is vertically inserted into the culture medium downwards, and the other end of the liquid separating pipe is vertically inserted into the anaerobic container; the second vent pipe is inserted into the anaerobic container and used for introducing oxygen-removing gas into the anaerobic container; the exposed pipe sections of the pipelines are respectively provided with a switch valve. The utility model discloses can solve the not enough of partial shipment in-process existence behind the anaerobe culture medium deoxidization.
Description
Technical Field
The utility model relates to an experimental device, more specifically say an anaerobe culture medium deaerating plant of area partial shipment function that says so.
Background
At present, the oxygen of an anaerobic bacteria culture medium is removed in a laboratory, the culture medium is generally subjected to manual sub-packaging by using a pipette and the like after being removed, and the phenomenon that oxygen is contacted in the operation process so that the oxygen of the culture medium is not completely removed exists, so that the cultured strict anaerobic bacteria cannot grow normally.
SUMMERY OF THE UTILITY MODEL
The utility model discloses aim at solving above-mentioned technical problem to a certain extent at least. Therefore, the utility model provides an anaerobe culture medium deaerating plant of area partial shipment function to can solve after the anaerobe culture medium deoxidization partial shipment in-process exist such as the deoxidization not thoroughly not enough.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model provides an anaerobe culture medium deaerating plant of area partial shipment function which structural feature is:
comprises a glass bottle, a first vent pipe, a liquid separating pipe, an exhaust check device, an anaerobic container and a second vent pipe;
the mouth of the glass bottle is sealed by a rubber plug, and the bottle is filled with a culture medium; the exhaust non-return device is used for receiving oxygen exhausted from the glass bottle, an exhaust port is reserved, and liquid paraffin is contained in the exhaust non-return device; the anaerobic container is used for receiving the culture medium discharged from the glass bottle and subjected to deoxygenation, and the bottle mouth can be sealed by a butyl rubber plug;
the vent pipe is vertically inserted into a culture medium in the glass bottle through the rubber plug, a pipe orifice exposed out of the bottle is used for introducing deoxygenation gas into the culture medium contained in the glass bottle, the exhaust pipe is communicated between the glass bottle and the exhaust check device, a pipe orifice at one end is inserted into the glass bottle through the rubber plug and is flush with the bottom end of the rubber plug, and a pipe orifice at the other end is vertically inserted into liquid paraffin in the exhaust check device; the liquid distribution pipe is communicated between the glass bottle and the anaerobic container, one end of the liquid distribution pipe is vertically downwards inserted into a culture medium in the glass bottle through the rubber plug, a gap is vertically reserved between the liquid distribution pipe and the bottom of the glass bottle, and the other end of the liquid distribution pipe is vertically downwards inserted into the anaerobic container through the butyl rubber plug; the vent pipe II is vertically inserted into the anaerobic container through a butyl rubber plug, and a pipe orifice exposed out of the bottle is used for introducing oxygen-removing gas into the anaerobic container;
and the exposed pipe sections of the first vent pipe, the liquid distribution pipe, the exhaust pipe and the second vent pipe are respectively provided with a switch valve.
The utility model discloses a structural feature also lies in:
when the glass bottle body is used for containing a liquid culture medium, the glass bottle body is cylindrical.
When the glass bottle is used for containing a solid culture medium, the glass bottle is a glass triangular flask movable round bottom flask.
The capacity of the glass bottle is 1-5L.
When the culture medium bottle is used for containing a solid culture medium, the culture medium bottle also comprises an electric heating furnace which is used for heating the glass bottle.
The oxygen-removed gas is oxygen-free nitrogen.
An air pipe which is communicated with the inner cavity and inclines upwards and outwards is formed at the upper end part of the exhaust check device and is used as the exhaust port communicated with the outside.
The first vent pipe, the liquid separating pipe and the exhaust pipe are all glass pipes.
The liquid distributing pipe is of a sectional type structure, and the two sections of pipe bodies are connected through a communicating pipe.
The bottle body of the anaerobic container is provided with scales and is an anaerobic tube with the capacity of less than 50mL or an anaerobic bottle with the capacity of 50-100 mL.
Compared with the prior art, the utility model discloses beneficial effect embodies:
the utility model discloses deoxidization and the automatic partial shipment of anaerobe culture medium can carry out, utilize the deoxidization gas to carry out the deoxidization back to the culture medium, recycle the culture medium pump income anaerobic container after the deoxidization in the gaseous atmospheric pressure effect of deoxidization in with the glass bottle, the partial shipment process adopts the mode of sealing the circulation, do not have the contact air and lead to the not thorough risk of culture medium deoxidization, and in complete back of culture medium partial shipment, supplement the culture medium through continuing toward the glass bottle, can realize continuous deoxidization, the partial shipment, the inconvenience and the culture oxygen infiltration risk nature brought of artifical manual partial shipment have been reduced, and is rational in infrastructure, and convenient for operation.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
In the figure, 1 glass bottle; 2, culture medium; 3, rubber plug; 4, a first vent pipe; 5, a liquid separating pipe; 6, exhausting the pipe; 7 exhaust check device; 8, an exhaust port; 9 liquid paraffin; 10 anaerobic container; 11, a second air pipe; 12 communicating pipes; 13 to open and close the valve.
Detailed Description
To make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the embodiments of the present invention are clearly and completely described below in combination with the technical solution of the embodiments of the present invention, and obviously, the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
The anaerobic bacteria culture medium deoxygenation device with the split charging function provided by the embodiment of the utility model is used for the deoxygenation of a large amount of culture medium 2 required by strict anaerobic bacteria and the split charging or tube split charging under the anaerobic condition so as to realize the strict deoxygenation before the sterilization of the culture medium 2, please refer to fig. 1, the device comprises a glass bottle 1, a first vent pipe 4, a liquid splitting pipe 5, an exhaust pipe 6, an exhaust non-return device 7, an anaerobic container 10 and a second vent pipe 11;
the mouth of the glass bottle 1 is sealed by a rubber plug 3, and the bottle is filled with a culture medium 2; the exhaust check device 7 is used for receiving oxygen exhausted from the glass bottle 1, an exhaust port 8 is reserved, and liquid paraffin 9 is contained in the exhaust check device; the anaerobic container 10 is used for receiving the culture medium 2 which is discharged from the glass bottle 1 and is deoxygenated, and the bottle mouth can be sealed by a butyl rubber plug;
the first vent pipe 4 is vertically inserted into the culture medium 2 in the glass bottle 1 through the rubber plug 3, a pipe orifice exposed outside the bottle is used for introducing oxygen-removing gas into the culture medium 2 contained in the glass bottle 1, the vent pipe 6 is communicated between the glass bottle 1 and the exhaust check device 7, a pipe orifice at one end is inserted into the glass bottle 1 through the rubber plug 3 and is flush with the bottom end of the rubber plug 3, and a pipe orifice at the other end is vertically inserted into liquid paraffin 9 in the exhaust check device 7; the liquid separating pipe 5 is communicated between the glass bottle 1 and the anaerobic container 10, one end of the liquid separating pipe is vertically downwards inserted into the culture medium 2 in the glass bottle 1 through the rubber plug 3, a gap is vertically reserved between the liquid separating pipe and the bottom of the glass bottle 1, and the other end of the liquid separating pipe is vertically downwards inserted into the anaerobic container 10 through the butyl rubber plug; the vent pipe II 11 is vertically inserted into the anaerobic container 10 through a butyl rubber plug, and a pipe orifice exposed outside the bottle is used for introducing oxygen-removing gas into the anaerobic container 10;
In specific implementation, the corresponding structural arrangement also includes:
when the glass bottle 1 is used for containing the liquid culture medium 2, the bottle body is cylindrical.
When the glass bottle 1 is used for containing the solid culture medium 2, the glass bottle is a glass triangular flask living round-bottom flask.
The glass bottle 1 has a capacity of 1-5L.
When the culture medium bottle is used for containing the solid culture medium 2, the culture medium bottle also comprises an electric heating furnace which is used for heating the glass bottle 1.
The oxygen-scavenging gas is oxygen-free nitrogen.
An air pipe which is communicated with the inner cavity and inclines upwards and outwards is formed at the upper end part of the exhaust non-return device 7 and is used as an exhaust port 8 communicated with the outside.
The first vent pipe 4, the liquid separating pipe 5 and the exhaust pipe 6 are all glass pipes.
The liquid distributing pipe 5 is of a sectional type structure, and the two sections of pipe bodies are connected through a communicating pipe 12. The connection pipe 12 is used to detachably connect the liquid separation pipe 5 between the glass bottle 1 and the anaerobic container 10, and is not limited to a glass tube, and is connected when the culture medium 2 is dispensed and detached when the culture medium 2 is replenished.
The anaerobic container 10 is provided with scales and is an anaerobic tube with the capacity of less than 50mL or an anaerobic bottle with the capacity of 50-100 mL.
The experiment can be operated as follows:
when the culture medium 2 is deaerated, the first vent pipe 4 is communicated with deaerated gas (anaerobic nitrogen), the switch valve 13 on the liquid distribution pipe 5 is closed at the moment, the anaerobic nitrogen entering the culture medium 2 in the glass bottle 1 enters the oxygen in the culture medium 2 into the exhaust non-return device 7 through the exhaust pipe 6, so that the oxygen in the culture medium 2 is discharged, when the anaerobic indicator of the culture medium 2 indicates the anaerobic condition, the anaerobic container 10 prepared in advance is connected with the liquid distribution pipe 5, the switch valve 13 on the second anaerobic nitrogen vent pipe 11 connected with the anaerobic container 10 is opened, the air in the bottle is removed, the switch valve 13 on the liquid distribution pipe 5 is opened, the switch valve 13 on the exhaust pipe 6 is closed, in the process, the first vent pipe 4 at the end of the glass bottle 1 keeps the circulation of the deaerated gas all the time, the pressure effect of the deaerated gas on the culture medium 2 in the glass bottle 1 is utilized to pump the culture medium 2 in the liquid distribution pipe 5 into the anaerobic container 10, after the subpackage culture medium 2 reaches the required volume scale, the switch valve 13 on the liquid distribution pipe 5 is closed, the new anaerobic container 10 is accessed, the continuous operation of the glass bottle 1, the continuous deaerated culture medium can be realized, and the continuous subpackage of the anaerobic culture medium can be realized, and the anaerobic liquid can be taken out, and the anaerobic culture medium can be taken from the glass bottle 2, and the glass bottle 2.
Example 1:
deoxygenation and split charging of liquid culture medium 2
The glass bottle 1 for containing the liquid culture medium 2 is a cylindrical glass bottle 1, the liquid culture medium 2 is deoxygenated, firstly, a resazurin oxygen indicator is added when the liquid culture medium 2 is prepared, the indicator is blue or dark blue when the indicator is oxygenated, the indicator is colorless when the indicator is not oxygenated, a first vent pipe 4 and a switch valve 13 on a vent pipe 6 are opened when the liquid culture medium 2 is deoxygenated, wherein the oxygen-free nitrogen introduced into the first vent pipe 4 is mainly used for deoxygenating the liquid culture medium 2, the vent pipe 6 is connected with an exhaust check device 7, an exhaust port 8 is arranged on the exhaust check device 7, liquid paraffin 9 is added in the exhaust check device 7, on one hand, the oxygen in the liquid culture medium 2 is favorably discharged, on the other hand, air can be prevented from flowing back into the liquid culture medium 2, the switch valve 13 on a liquid separation pipe 5 is closed, deoxygenation is carried out, and when the color of the liquid culture medium 2 is changed into colorless, the oxygen in the liquid culture medium 2 is completely removed.
The anaerobic bottle for subpackaging the liquid culture medium 2 is characterized in that before subpackaging the liquid culture medium 2, firstly, a switch valve 13 on a vent pipe II 11 is opened, anaerobic nitrogen is introduced, the anaerobic bottle is deoxidized for about 1-3min, then a communicating pipe 12 is utilized to communicate a liquid separating pipe 5 between a glass bottle 1 and the anaerobic bottle, the switch valve 13 on an exhaust pipe 6 is closed, the switch valve 13 on the liquid separating pipe 5 is opened, the liquid culture medium 2 after deoxidization in the glass bottle 1 is continuously pumped into the anaerobic bottle to the required volume scale under the action of the anaerobic nitrogen pressure introduced through the vent pipe I4, then the switch valve 13 on the exhaust pipe 6 is opened, a butyl rubber plug of the anaerobic bottle is plugged, and an aluminum foil cover is pressed on by a capping device.
And (4) continuously subpackaging according to the subpackaging steps until the liquid culture medium 2 in the glass bottle 1 is completely subpackaged.
Example 2:
deoxygenation and split charging of solid culture medium 2
The glass bottle 1 for containing the solid culture medium 2 is a glass triangular bottle, the solid culture medium 2 is deoxygenated, firstly, a resazurin oxygen indicator is added when the solid culture medium 2 is prepared, and the indicator is blue or dark blue when oxygen exists and is colorless when oxygen does not exist. When the solid culture medium 2 is deoxygenated, the glass triangular bottle filled with the solid culture medium 2 is placed on an electric heating furnace, the power is as small as possible, the solid culture medium 2 is kept in a liquid state, the vent pipe I4 and the switch valve 13 on the vent pipe 6 are opened, wherein, oxygen-free nitrogen is introduced into the glass bottle 1 through the vent pipe I4 and is mainly used for deoxygenation of the culture medium 2, the vent pipe 6 is connected with the exhaust non-return device 7, the exhaust non-return device 7 is provided with the exhaust port 8, liquid paraffin 9 is added into the exhaust non-return device 7, on one hand, the oxygen in the culture medium 2 is favorably discharged, on the other hand, air can be prevented from flowing back to the culture medium 2, the switch valve 13 on the liquid distribution pipe 5 is closed, deoxygenation is carried out, and when the color of the culture medium 2 is changed into colorless, the oxygen in the solid culture medium 2 is completely removed.
The anaerobic tube for the sub-packaging of the solid culture medium 2 is placed on a test tube rack during deoxygenation and sub-packaging, before the sub-packaging of the culture medium 2, firstly, a switch valve 13 IV on a vent pipe II 11 is opened, anaerobic nitrogen is introduced into the anaerobic tube, deoxygenation is performed on the anaerobic tube for about 1min, then a communicating pipe 12 is used for connecting a liquid distributing pipe 5 between a glass bottle 1 and the anaerobic bottle, the switch valve 13 on the vent pipe 6 is closed, the switch valve 13 on the liquid distributing pipe 5 is opened, the solid culture medium 2 after deoxygenation in the glass bottle 1 is automatically pumped into the anaerobic tube to the required volume scale under the pressure action of the anaerobic nitrogen continuously introduced through a vent pipe I4, then the switch valve 13 on the vent pipe 6 is opened, a butyl rubber plug of the anaerobic tube is plugged, and an aluminum foil cover is pressed by a capping device.
And continuously subpackaging according to the subpackaging steps until the solid culture medium 2 in the glass bottle 1 is completely subpackaged.
While embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.
Claims (10)
1. The utility model provides an anaerobe culture medium deaerating plant of area partial shipment function, characterized by:
comprises a glass bottle, a first vent pipe, a liquid separating pipe, an exhaust check device, an anaerobic container and a second vent pipe;
the mouth of the glass bottle is sealed by a rubber plug, and the bottle is filled with a culture medium; the exhaust non-return device is used for receiving oxygen exhausted from the glass bottle, an exhaust port is reserved, and liquid paraffin is contained in the exhaust non-return device; the anaerobic container is used for receiving the culture medium which is discharged from the glass bottle and is deoxygenated, and the bottle mouth can be sealed by a butyl rubber plug;
the vent pipe is vertically inserted into a culture medium in the glass bottle through the rubber plug, a pipe orifice exposed out of the bottle is used for introducing deoxygenation gas into the culture medium contained in the glass bottle, the exhaust pipe is communicated between the glass bottle and the exhaust check device, a pipe orifice at one end is inserted into the glass bottle through the rubber plug and is flush with the bottom end of the rubber plug, and a pipe orifice at the other end is vertically inserted into liquid paraffin in the exhaust check device; the liquid distribution pipe is communicated between the glass bottle and the anaerobic container, one end of the liquid distribution pipe is vertically inserted into a culture medium in the glass bottle through the rubber plug, a gap is vertically reserved between the liquid distribution pipe and the bottom of the glass bottle, and the other end of the liquid distribution pipe is vertically inserted into the anaerobic container; the second vent pipe is vertically inserted into the anaerobic container, and a pipe orifice exposed out of the bottle is used for introducing oxygen-removing gas into the anaerobic container;
switch valves are respectively arranged on exposed pipe sections of the first vent pipe, the liquid distributing pipe, the exhaust pipe and the second vent pipe.
2. The anaerobic culture medium oxygen removing device with the subpackaging function as recited in claim 1, which is characterized in that: when the glass bottle body is used for containing a liquid culture medium, the glass bottle body is cylindrical.
3. The anaerobic culture medium oxygen removing device with the subpackaging function as recited in claim 1, which is characterized in that: when the glass bottle is used for containing a solid culture medium, the glass bottle is a glass triangular flask living round-bottom flask.
4. The anaerobic bacteria culture medium oxygen removing device with the subpackaging function as claimed in claim 1, which is characterized in that: the capacity of the glass bottle is 1-5L.
5. The anaerobic culture medium oxygen removing device with a subpackaging function according to claim 1 or 3, which is characterized in that: when the culture medium bottle is used for containing a solid culture medium, the culture medium bottle also comprises an electric heating furnace which is used for heating the glass bottle.
6. The anaerobic culture medium oxygen removing device with the subpackaging function as recited in claim 1, which is characterized in that: the oxygen-removed gas is oxygen-free nitrogen.
7. The anaerobic bacteria culture medium oxygen removing device with the subpackaging function as claimed in claim 1, which is characterized in that: an air pipe which is communicated with the inner cavity and inclines upwards and outwards is formed at the upper end part of the exhaust check device and is used as the exhaust port communicated with the outside.
8. The anaerobic bacteria culture medium oxygen removing device with the subpackaging function as claimed in claim 1, which is characterized in that: the first vent pipe, the liquid separating pipe and the exhaust pipe are all glass pipes.
9. The anaerobic bacteria culture medium oxygen removing device with the subpackaging function as claimed in claim 1, which is characterized in that: the liquid distributing pipe is of a sectional type structure, and the two sections of pipe bodies are connected through a communicating pipe.
10. The anaerobic culture medium oxygen removing device with the subpackaging function as recited in claim 1, which is characterized in that: the bottle body of the anaerobic container is provided with scales and is an anaerobic tube with the capacity of less than 50mL or an anaerobic bottle with the capacity of 50-100 mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221602828.0U CN218620761U (en) | 2022-06-24 | 2022-06-24 | Anaerobic bacteria culture medium deaerating plant of area partial shipment function |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221602828.0U CN218620761U (en) | 2022-06-24 | 2022-06-24 | Anaerobic bacteria culture medium deaerating plant of area partial shipment function |
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| CN218620761U true CN218620761U (en) | 2023-03-14 |
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| CN202221602828.0U Active CN218620761U (en) | 2022-06-24 | 2022-06-24 | Anaerobic bacteria culture medium deaerating plant of area partial shipment function |
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- 2022-06-24 CN CN202221602828.0U patent/CN218620761U/en active Active
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