CN218572874U - Separation column integrated health-care product total saponin extraction device - Google Patents
Separation column integrated health-care product total saponin extraction device Download PDFInfo
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- CN218572874U CN218572874U CN202222413818.9U CN202222413818U CN218572874U CN 218572874 U CN218572874 U CN 218572874U CN 202222413818 U CN202222413818 U CN 202222413818U CN 218572874 U CN218572874 U CN 218572874U
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Abstract
The utility model relates to a health products extraction element technical field discloses a total saponin extraction element of health products of separation column integration, including extracting bottle, separation column and constant volume bottle, there is the dull polish charge door on extracting the bottle, and the lower extreme has the dull polish discharge gate, and there is a piston control at the dull polish discharge gate middle part, and the separation column is connected to dull polish discharge gate lower extreme, and the lower extreme of separation column has separation column piston control, and the separation column lower extreme is connected and is decided the appearance bottle. The extraction bottle extracts active ingredients in the health care product to form an extracting solution, the extracting solution is directly connected to the separation column to be separated, the extracting solution flows into the constant volume bottle to directly measure the total saponin concentration in a constant volume mode, and the health care product is stored in the extraction bottle from the beginning, so that the transfer step of the extracting solution is reduced, and the loss of the extracting solution caused in the transfer process is also reduced.
Description
Technical Field
The utility model relates to a health products extraction element technical field especially relates to a health products total saponin extraction element of separation post integration.
Background
The health product comprises various chemical substances beneficial to human body, wherein saponin is a common health product component, is extracted from the health product, passes through a separation column, and is compounded by using organic solvents with different polarities and water, so that the effects of separating protein, amino acid, polysaccharide and saponin in the health product extracting solution can be achieved.
For example, chinese patent CN101200487B provides a "method for preparing asiatic centella total saponins by using macroporous adsorption resin", which adopts macroporous adsorption resin to extract total saponins from centella, but the total saponins are often separated only by changing the polarity of the solvent, and meanwhile, in the process of processing health food in factories, organic solvent and heavy metal ion residues may occasionally exist, the separation effect of saponins by using a separation column of a single resin is limited, and the extract may be lost when being transferred to the separation column.
Disclosure of Invention
In order to solve the problem of material loss caused by transferring the extracting solution to the separation column, the invention provides the separation column integrated health-care product total saponin extraction device, which can efficiently complete the purification of total saponin and reduce the transferring steps of the extracting solution so as to reduce the loss in the transferring process.
The utility model discloses realize with following technical scheme:
the utility model provides a total saponin extraction element of health products of separation column integration, is including extracting bottle, separator and constant volume bottle, and there is the dull polish charge door extraction bottle upper end, and the lower extreme has the dull polish discharge gate, and there is extraction bottle piston control frosted discharge gate middle part, and the separator is connected to dull polish discharge gate lower extreme, and the lower extreme of separator has separator piston control, and the separator lower extreme is connected and is decided the appearance bottle.
The extraction bottle extracts active ingredients in the health care product to form an extracting solution, the extracting solution is directly connected to the separation column to be separated, the extracting solution flows into the constant volume bottle to directly measure the total saponin concentration in a constant volume mode, and the health care product is stored in the extraction bottle from the beginning, so that the transfer step of the extracting solution is reduced, and the loss of the extracting solution caused in the transfer process is also reduced.
Preferably, the separation column is formed by splicing a plurality of sections of single separation columns, a filter structure is arranged on the transverse contact surface of each section of single separation column, the filler is arranged among the filter structures, and the single separation columns are connected by joints; the filtering structure separates different fillers in the single separation column, and can be eluted at one time to achieve different separation effects.
Preferably, the joint is a concave end and a convex end which are respectively arranged at the upper end and the lower end of the single separation column, the outer side of the convex end and the inner side of the concave end are provided with concave-convex end threads which are matched, the convex end and the concave end are spliced together and screwed together, the concave end is opened upwards, the convex end is combined with the concave end downwards, and the joint is not easy to backflow.
Preferably, at least one of the convex end and the concave end of the single separation column is detachably connected with the column wall of the single separation column, and the convex end and the concave end can be clamped and sealed with the single separation column and can be detached from the single separation column, so that the single separation column can be filled with the filling material conveniently.
Preferably, the concave end outer side wall in the detachable connection is connected with the inner side wall of the lower single-separation column, the convex end inner side wall in the detachable connection is connected with the outer side wall of the upper single-separation column, and the convex end and/or the concave end and the single-separation column are/is provided with column wall threads matched with each other on the wall surface in mutual contact, so that the connection mode can reduce liquid backflow in the column.
Preferably, the filtering structure is a sand core, and the sand core is a structure with the pore diameter larger than that of the protein and the polysaccharide but smaller than the particle diameter of the filler, so that the filler is wrapped in the sand core, and the outflow of the extracting solution is not hindered.
Preferably, each single separation column is different in length, and the single separation columns with different lengths can be selected by workers according to different requirements.
Preferably, the filler in the separation column comprises one or more of ion exchange resin, gel type exchange resin, macroporous adsorption resin, alumina, silica gel, graphitized carbon, and carbon nanofibers.
The extracting solution obtained from the health-care product through crude extraction contains a plurality of proteins, amino acids, polysaccharides and total saponins, and possible residues of organic solvents and heavy metal ions, wherein the molecular weights of the proteins and the polysaccharides are large, the amino acids and the total saponins have different polarities due to different molecular structures, the proteins and the polysaccharides can be separated by using a macroporous adsorption separation column, and then the finely-compounded eluent between the organic solvents and water is designed according to the polarity of the total saponins, so that the total saponins can be effectively and accurately eluted, meanwhile, the carbon nanofibers can well adsorb toxic substances after being oxidized and connected with a catalyst, and the heavy metal ions can be removed by using an ionic separation column, so that different fillers are filled into multiple single-section separation columns to connect the multiple single-section separation columns, and the extracting solution passes through multiple different single-section separation columns once, so that the separation efficiency can be improved, and the separation accuracy cannot be lost.
Preferably, each single separation column is packed with a different packing material; the extracting solution passes through each single separation column from top to bottom through an isolation net, and various fillers are not interfered with each other; according to the different impurities to be removed, different fillers are selected, molecular imprinting type fillers can be selected for different molecules which have similar polar molecular weights and are difficult to separate, fine compounding of a solvent is adjusted, the single separation columns are disassembled and independently eluted to achieve the effect of accurate separation, and when the single separation columns are disassembled, different chemical substances left in the single separation columns are independently eluted and collected again, so that the worry that the different chemical substances are mixed with other chemical substances again is avoided.
Preferably, a sand core at the lowest end of the single separation column at the lowest end of the separation column is connected with a sand outlet, the diameter of the sand outlet is smaller than that of each single separation column, so that effluent liquid can be conveniently collected, and the piston controls liquid in the column to flow downwards.
Preferably, the lower part of the extraction bottle is connected with a ampulla bottle, the lower end of the ampulla bottle is connected with the single separation column, and the ampulla bottle is of an expanding structure and is favorable for temporarily storing the extracting solution.
The utility model discloses beneficial effect: (1) The separation column is directly connected with the extraction bottle, so that the material transfer step is omitted, and the material loss is reduced; (2) The separation column is formed by assembling a plurality of single separation columns containing different fillers, can achieve the separation effect of separating a plurality of different impurities including toxic organic solvents and heavy metal ions, reduces the accuracy requirement of compounding an eluent by using an organic solvent, and effectively improves the separation accuracy; (3) The single separation column can be disassembled, can be flexibly assembled according to different separation requirements, and can meet the actual separation conditions of different Chinese herbal medicines; (4) The fillers in the single separation columns are not interfered with each other, different chemical substances in different single separation columns can be eluted and collected independently, and the separation accuracy is not influenced.
Drawings
FIG. 1 is a schematic view of an integrated structure of a separation column.
FIG. 2 is a side view of two single separation columns in isolation.
Fig. 3 is a schematic view of the structure of the extraction bottle connected with the condenser tube.
1-extracting bottle; 2-ampulla; 3-a separation column; 4-single separation column; 5-sand core; 6-a linker; 6.1-convex end; 6.2-concave end; 7-extraction bottle piston; 8-a frosted feed inlet; 9-frosted discharge hole; 10-a fixed-volume bottle; 11-a frosted outflow opening; 12-a separator piston; 13-concave-convex end threads; 14-column wall threads; 15-a condenser tube; 16-neutral alumina column; 17-macroporous adsorbent resin column; an 18-ionic separation column; 19-carbon nanofiber column.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and the detailed description.
Example 1
A total saponin extraction device of a health care product integrated with a separation column is shown in figure 1 and comprises an extraction bottle 1, a separation column 3 and a constant volume bottle 10, wherein the upper end of the extraction bottle 1 is provided with a frosted feeding port 8, the lower end of the extraction bottle is provided with a frosted discharge port 9, the middle part of the frosted discharge port 9 is controlled by an extraction bottle piston 7, the lower end of the frosted discharge port 9 is connected with the separation column 3, the lower end of the separation column 3 is controlled by a separation column piston 12, the lower end of the separation column 3 is connected with the constant volume bottle 10, the separation column 3 is divided into a plurality of sections of single separation columns 4 which are assembled, the transverse contact surface of each section of single separation column 4 is provided with a sand core 5, a filler is arranged between the sand cores 5, the pore diameter of the sand core 5 is larger than each component of the extract but smaller than the particle diameter of the filler, and does not obstruct the outflow of the extract; the single separation columns 4 are connected by joints 6, the separation column 3 is composed of a neutral alumina column 16 filled with neutral alumina, an ionic type separation column 18 filled with ionic type exchange resin, a carbon nanofiber column 19 filled with carbon oxide nanofibers connected with a catalyst and a macroporous adsorption resin column 17 filled with macroporous adsorption resin in sequence from top to bottom, wherein the carbon oxide nanofibers connected with the catalyst come from Chinese patent CN109157867B, the extracting solution passes through the single separation columns 4 filled with a plurality of different fillers at one time to play a role in improving the separation efficiency without losing the separation precision, and the frosted feed inlet 8 and the frosted discharge outlet 9 are beneficial to the close connection of the extracting bottle 1 and a device below the extracting bottle to prevent liquid from seeping out; the lower end of the sand core 5 at the bottommost end of the single separation column 4 at the bottommost end of the separation column 3 is a frosted outflow port 11, a separation column piston 12 is arranged on the frosted outflow port 11, the diameter of the outflow port 11 is smaller than that of each single separation column 4, so that effluent liquid can be conveniently collected, and the two pistons jointly control liquid in the columns to flow downwards; draw 1 below of bottle and still be connected with ampulla bottle 2, ampulla bottle 2 lower extreme is connected with single-separating column 3, and ampulla bottle 2 is an enlarged structure, is favorable to temporarily storing the extract.
As shown in fig. 2, the joint 6 is a convex end 6.1 and a concave end 6.2 which are respectively arranged at the lower end and the upper end of the single separation column 4, the outer side of the convex end 6.1 and the inner side of the concave end 6.2 are provided with matched concave-convex end threads 13, the convex end 6.1 is spliced with the concave end 6.2 under the concave end 6.2 and screwed together, and liquid in the column is not easy to flow back from a joint surface; the convex end 6.1 and the concave end 6.2 of the single separation column 4 are detachably connected with the column wall of the single separation column 4 per se, the outer side wall of the concave end 6.2 is connected with the inner side wall of the single separation column 4 below, the inner side wall of the convex end 6.1 is connected with the outer side wall of the single separation column 4 above, and adaptive column wall threads 14 are arranged on the mutual contact wall surfaces of the convex end 6.1 and the concave end 6.2 with the single separation column 4, so that the convex end 6.1 and the concave end 6.2 can be detached from the single separation column 4 for filling packing, and liquid in the column is not easy to flow back from a joint surface; the single separation columns 4 are different in length and can be selected according to needs.
The working process of the invention is as follows:
1. sample extraction and reflux: as shown in figure 3, accurately weighing health product, pulverizing, sieving, placing into an extraction bottle 1, connecting with a condenser tube 15, adding water, heating in boiling water bath for reflux, extracting total saponins from the sample, and cooling;
2. column passing: preparation of the separation column 3: connecting a ampulla bottle 2 above the separation column 3, connecting the lowest end of the separation column 2 with a constant volume bottle 10 through a frosted outflow port 11, washing the column with ethanol, discarding the eluent, washing the column with water, discarding the eluent collected in the constant volume bottle 10, connecting the ampulla bottle 2 to the upper end of the separation column 3, taking the extraction bottle 1 down from a condensation pipe 15, connecting the extraction bottle 2 with the ground outflow port 9, opening a piston 7 of the extraction bottle, enabling the extraction solution to pass through the column, discarding the filtrate collected in the constant volume bottle 10, detaching the extraction bottle 1, adding water into the ampulla bottle 2 to wash the column, discarding the water collected in the constant volume bottle 10, then washing off the saponin in the column with 70% ethanol, and collecting the saponin in the constant volume bottle 10;
3. volume fixing: and (3) fixing the volume of the saponin ethanol solution in the volume fixing bottle 10, sucking a certain amount of the saponin ethanol solution, and performing color development and standard solution color comparison to obtain the concentration of the total saponin in the extracting solution.
Claims (10)
1. The utility model provides a total saponin extraction element of health products of separation column integration, is including drawing bottle, separation column and surely holding the bottle, its characterized in that, draws the bottle upper end and has the dull polish charge door, and the lower extreme has the dull polish discharge gate, and there is a piston control of drawing bottle at dull polish discharge gate middle part, and the separation column is connected to dull polish discharge gate lower extreme, and the lower extreme of separation column has separation column piston control, and the separation column lower extreme is connected and is surely held the bottle.
2. The device for extracting total saponins in a health-care product integrated with a separation column as claimed in claim 1, wherein the separation column is formed by assembling a plurality of sections of single separation columns, a filter structure is arranged on the transverse contact surface of each section of single separation column, a filler is arranged among the filter structures, and the single separation columns are connected by joints.
3. The device for extracting total saponins in health products with integrated separation column as claimed in claim 2, wherein the connectors are a concave end and a convex end respectively arranged at the upper end and the lower end of the single separation column, and the outer side of the convex end and the inner side of the concave end are provided with matched concave-convex end threads.
4. The device for extracting total saponins from health products with integrated separation column as claimed in claim 3, wherein at least one of the convex end and the concave end of the single separation column is detachably connected with the column wall of the single separation column.
5. The device for extracting total saponins in health care products integrated with separation column according to claim 4, characterized in that the outer side wall of the concave end in the detachable connection is connected with the inner side wall of the single separation column below, the inner side wall of the convex end in the detachable connection is connected with the outer side wall of the single separation column above, and adaptive column wall threads are arranged on the wall surfaces of the convex end and/or the concave end, which are in mutual contact with the single separation column.
6. The device for extracting total saponins in health products with integrated separation column as claimed in claim 2, wherein the filtering structure is a sand core.
7. The device for extracting total saponins in a health product integrated with a separation column as claimed in any one of claims 1 to 6, wherein the filler in the separation column comprises one or more of ion exchange resin, gel exchange resin, macroporous adsorption resin, alumina, silica gel, graphitized carbon and carbon nanofibers.
8. The device for extracting total saponins in health products with integrated separation column as claimed in claim 7, wherein each single separation column is filled with a different filler.
9. The device for extracting total saponins in health care products integrated with separation columns as claimed in claim 6, wherein the sand core at the lowest end of the single separation column at the lowest end of the separation column is connected with a sand outlet with a diameter smaller than that of each single separation column.
10. The device for extracting total saponins from health products with integrated separation column as claimed in claim 1, wherein a ampulla bottle is connected below the extraction bottle, and the lower end of the ampulla bottle is connected with the single separation column.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222413818.9U CN218572874U (en) | 2022-09-09 | 2022-09-09 | Separation column integrated health-care product total saponin extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222413818.9U CN218572874U (en) | 2022-09-09 | 2022-09-09 | Separation column integrated health-care product total saponin extraction device |
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| CN218572874U true CN218572874U (en) | 2023-03-07 |
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| CN202222413818.9U Active CN218572874U (en) | 2022-09-09 | 2022-09-09 | Separation column integrated health-care product total saponin extraction device |
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