CN218520084U

CN218520084U - Magnetic bead method tissue/cell DNA extraction kit

Info

Publication number: CN218520084U
Application number: CN202222397553.8U
Authority: CN
Inventors: 郭陆军; 邹春平; 鲁明霞; 张义强; 刘洪�
Original assignee: Henan Taifu Medical Laboratory Center Co ltd
Current assignee: Henan Taifu Medical Laboratory Center Co ltd
Priority date: 2022-09-09
Filing date: 2022-09-09
Publication date: 2023-02-24
Anticipated expiration: 2032-09-09

Abstract

The utility model relates to a magnetic bead method tissue/cell DNA extraction kit, a box body comprises a plurality of reagent grooves and a jacking device; the jacking device comprises a fixed seat, a jacking seat, a supporting spring, a jacking air bag and a first inflation unit for inflating the jacking air bag; the pushing air bag is provided with an exhaust valve which can be opened and closed; the first inflation unit comprises a first piston mechanism and a first air cavity arranged at the air inlet end of the first piston mechanism; the first air cavity is provided with a first one-way valve for guiding air flow to enter the first air cavity and a second one-way valve for guiding air flow to enter the pushing air bag from the first air cavity. The utility model has the advantages of reasonable design, the staff has inclined plane slidable footstock through designing at reagent tank bottom, hides the protection completely to the reagent bottle when the storage, reduces the risk of reagent bottle collision, carries out synchronous top to the reagent bottle when using and pushes away and rise, reduces the degree of difficulty that the staff took the reagent bottle, reduces the risk that the staff adopted anchor clamps rigidity centre gripping reagent bottle.

Description

Magnetic bead method tissue/cell DNA extraction kit

Technical Field

The utility model belongs to the technical field of the kit and specifically relates to a magnetic bead method tissue/cell DNA draws kit.

Background

The kit is used for containing chemical reagents for detecting chemical components, drug residues, virus types and the like, and the magnetic bead method DNA extraction kit is a box for storing the DNA extraction reagent and is used for extracting pure nucleic acid from samples such as plasma, serum, ascites, tissue grinding supernatant, cultured cell supernatant, exfoliated cell suspension, swabs and the like so as to be used for subsequent molecular diagnosis. Chinese patent document of grant publication No. CN207987181U discloses a magnetic bead method virus DNA/RNA extraction kit, including box body and lid, the bottom fixedly connected with electromagnetic plate of box inner chamber, the top swing joint of electromagnetic plate has reagent frame, and the right side of box body set up with the logical groove of reagent frame looks adaptation, the thing groove has been seted up to the inside of reagent frame, the inside sliding connection who puts the thing groove has the tray, and the bottom of tray through first spring with put the bottom fixed connection in thing groove, put the left and right sides in thing groove and all seted up the rotation groove, and the internal surface rotation that rotates the groove is connected with the baffle, the utility model relates to a kit technical field. This magnetic bead method virus DNA/RNA draws kit utilizes and presses cover and push rod cooperation, can push up the ejector pin downwards, and then makes the baffle break away from the fixture block, through first spring and tray cooperation, can be automatic with reagent pipe upper and lower promotion, easy operation is convenient, and can take out corresponding reagent pipe, can not influence other reagent pipes, preserves effectually. But the medicine hole that current kit was seted up is darker, and the user generally need press from both sides with the help of anchor clamps and get the reagent bottle in the reagent of taking, and the anchor clamps centre gripping in-process causes the reagent bottle damaged easily, pollutes kit and reagent, causes the work easily and repeats, the wasting of resources.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a magnetic bead method tissue/cell DNA draws kit for the medicine hole that current kit of solution had was offered is darker, and the user generally need press from both sides with the help of anchor clamps and get the reagent bottle in the reagent of taking, causes the reagent bottle damaged easily in the anchor clamps centre gripping process, pollutes kit and reagent, causes the work easily repeatedly, the problem of extravagant resource.

In order to solve the problems, the utility model provides a magnetic bead method tissue/cell DNA extraction kit, a box body comprises a plurality of reagent grooves for holding reagent bottles and a jacking device which is arranged at the bottom of the reagent grooves and can jack the reagent bottles; the jacking device comprises a fixed seat fixed at the bottom of the box body for supporting a reagent bottle, a top seat which can horizontally slide and is close to the fixed seat for jacking the bottom of the reagent bottle, a supporting spring which is arranged on the fixed seat and is used for jacking and separating the top seat, a jacking air bag for jacking the top seat to be close to the supporting spring, and a first inflation unit for inflating the jacking air bag; the pushing air bag is provided with an exhaust valve which can be opened and closed; the first inflation unit comprises a first piston mechanism and a first air cavity arranged at the air inlet end of the first piston mechanism; the first air cavity is provided with a first one-way valve for guiding air flow to enter the first air cavity and a second one-way valve for guiding air flow to enter the pushing air bag from the first air cavity.

The utility model provides a magnetic bead method tissue-cell DNA draws kit still has following technical characteristics:

furthermore, a positioning device for positioning and supporting the reagent bottle is arranged in the box body; the positioning device comprises a plurality of airbag chambers arranged around the reagent tank, supporting airbags arranged in the airbag chambers, and a second inflating unit communicated with the supporting airbags; a plurality of supporting holes which are communicated with the air bag chamber and the inner cavity of the reagent groove and can be used for the supporting air bags to pass through are arranged on the side wall of the reagent groove; the second inflation unit comprises a second piston mechanism and a second air cavity arranged at the air inlet end of the second piston mechanism; and a third one-way valve for guiding outside air flow into the second air cavity and a fourth one-way valve for guiding air flow from the second air cavity into the supporting air bag are arranged on the second air cavity.

Further, the air inlet end of the first one-way valve is synchronously communicated with the plurality of supporting air bags through a pipeline.

Furthermore, the first piston mechanism comprises two piston cylinders which can be lifted synchronously and a lifting handle which is connected with handles of the two piston cylinders; the piston in the piston cylinder can only slide in a reciprocating manner in the piston cylinder; the second piston mechanism is identical in structure to the first piston mechanism.

Furthermore, the top of the fixed seat is provided with a flexible gasket for supporting the reagent bottle.

Furthermore, a plurality of supporting blocks arranged at intervals are arranged on the fixed seat; a plurality of inserting blocks capable of being inserted into the gaps of the supporting blocks are arranged on the top seat; the supporting spring is arranged in the gap between the two supporting blocks.

Furthermore, the top of the supporting block is provided with a supporting surface which inclines downwards along the direction close to the top seat.

Furthermore, the top of the inserting block is provided with a supporting surface which obliquely supports the reagent bottle downwards along the direction close to the supporting spring.

Furthermore, a cover body for detachably plugging the opening of the box body is arranged at the top of the box body; the box body is provided with a plurality of threaded rods; the cover body is provided with a through hole for the threaded rod to pass through; and the cover body is provided with a flexible plugging convex ring matched with the inner diameter of the reagent groove.

The utility model discloses following beneficial effect has: the utility model has the advantages of reasonable design and simple structure, the staff completely hides the protection to the reagent bottle during storage through design at the bottom of the reagent tank with the slidable footstock of inclined plane, reduces the risk of reagent bottle collision, and when in use, the reagent bottle is pushed up synchronously, thereby reducing the difficulty of taking the reagent bottle by the staff and reducing the risk of clamping the reagent bottle by the staff by adopting the fixture; the inflatable positioning device supports and protects the reagent bottle storage, so that the risk of collision of the reagent bottle in the storage and movement process is reduced; the gas circuit through the intercommunication is when jacking the reagent bottle, and positioner releases the reagent bottle, guarantees that the reagent bottle stably goes up and down safety protection, and has improved the utilization ratio of equipment, guarantees to the safe storage of reagent bottle.

Drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present invention;

fig. 2 is a structural sectional view of an embodiment of the present invention;

fig. 3 is an exploded view of the embodiment of the present invention;

in the figure: 1-box body, 11-threaded rod, 2-cover body, 21-through hole, 22-blocking convex ring, 3-reagent groove, 31-supporting hole, 4-positioning device, 41-air bag chamber, 42-supporting air bag, 43-second inflating unit, 431-second piston mechanism, 432-second air chamber, 433-third one-way valve, 434-fourth one-way valve, 5-jacking device, 51-fixing seat, 511-supporting block, 52-top seat, 521-inserting block, 53-supporting spring, 54-jacking air bag, 541-exhaust valve, 55-first inflating unit, 551-first piston mechanism, 552-first air chamber, 553-first one-way valve, 554-second one-way valve, 555-piston cylinder and 556-lifting handle.

Detailed Description

The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments. It should be noted that, in the case of no conflict, the embodiments and features of the embodiments of the present invention may be combined with each other.

In one embodiment of the tissue/cell DNA extraction kit according to the magnetic bead method of the present invention as shown in fig. 1 to 3, the kit body 1 comprises a plurality of reagent tanks 3 for containing reagent bottles, and a lifting device 5 disposed at the bottom thereof for lifting up the reagent bottles; the jacking device 5 comprises a fixed seat 51 fixed at the bottom of the box body 1 for supporting a reagent bottle, a top seat 52 capable of horizontally sliding and approaching the fixed seat 51 to jack the bottom of the reagent bottle, a supporting spring 53 arranged on the fixed seat 51 for jacking and separating the top seat 52, a jacking air bag 54 for jacking the top seat 52 to approach the supporting spring 53, and a first inflation unit 55 for inflating the jacking air bag 54; an exhaust valve 541 capable of being opened and closed is arranged on the pushing air bag 54; the first air charging unit 55 comprises a first piston means 551, a first air chamber 552 arranged at the air inlet end of the first piston means 551; the first air chamber 552 is provided with a first check valve 553 for guiding the air flow into the interior thereof and a second check valve 554 for guiding the air flow from the first air chamber 552 into the pusher air chamber 54.

Specifically, a positioning device 4 for positioning and supporting the reagent bottle is arranged in the box body 1; the positioning device 4 comprises a plurality of air bag chambers 41 arranged around the reagent tank 3, supporting air bags 42 arranged in the air bag chambers 41, and a second inflating unit 43 communicated with the plurality of supporting air bags 42; a plurality of supporting holes 31 which are communicated with the air bag chamber 41 and the inner cavity of the reagent tank 3 and can be used for supporting the air bag 42 to pass through are arranged on the side wall of the reagent tank 3; the second inflation unit 43 includes a second piston mechanism 431, a second air chamber 432 provided at an inlet end of the second piston mechanism 431; the second air chamber 432 is provided with a third check valve 433 for guiding the outside air flow into the inside thereof and a fourth check valve 434 for guiding the air flow from the second air chamber 432 into the support airbag 42.

Specifically, the intake end of the first check valve 553 is in synchronous communication with the plurality of support airbags 42 through a pipe.

Specifically, the first piston mechanism 551 comprises two piston cylinders 555 which can be lifted and lowered synchronously, and a lifting handle 556 which is connected with handles of the two piston cylinders 555; the piston in the piston cylinder 555 can only slide back and forth in the piston cylinder 555; the second piston mechanism 431 is identical in structure to the first piston mechanism 551.

Specifically, a flexible gasket for supporting the reagent bottle is disposed on the top of the fixing base 51.

Specifically, the fixing base 51 is provided with a plurality of supporting blocks 511 arranged at intervals; the top seat 52 is provided with a plurality of inserting blocks 521 which can be inserted into the gaps of the supporting blocks 511; the support spring 53 is disposed in the gap between the two support blocks 511.

Specifically, the top of the supporting block 511 is provided with a supporting surface inclined downward in a direction approaching the top seat 52.

Specifically, the top of the insert 521 is provided with a support surface for supporting the reagent bottle obliquely downward in a direction approaching the support spring 53.

Specifically, the top of the box body 1 is provided with a cover body 2 which can be detached to seal the opening of the box body 1; the box body 1 is provided with a plurality of threaded rods 11; the cover body 2 is provided with a through hole 21 for the threaded rod 11 to pass through; the cover body 2 is provided with a flexible plugging convex ring 22 matched with the inner diameter of the reagent groove 3.

The working principle is as follows: when the reagent kit is used by a worker, after the screw cap on the threaded rod 11 is removed, the cover body 2 is removed to unseal the reagent tank 3, the worker pulls the first piston mechanism 551, the piston moves to drive the gas in the supporting air bag 42 for supporting the reagent bottle to enter the pushing air bag 54 through the first check valve 553, the first air cavity 552 and the second check valve 554 in sequence, so as to release the reagent bottle in the reagent tank 3, the pushing air bag pushing top seat 52 extrudes the supporting spring 53, so that the supporting surface inclined on the insert block 521 pushes the bottom of the reagent bottle, the reagent bottle mouth is pushed out of the reagent tank 3, the worker can conveniently clamp the reagent bottle by hand, the inconvenience of clamping the reagent bottle by the clamp is avoided, after the worker finishes using the reagent bottle, the reagent bottle is put into the reagent tank 3 again, the bottom of the reagent bottle is buffered under the support of the flexible gasket at the top of the insert block 521, the impact of the bottom of the reagent bottle is reduced, after all the reagent bottles are reset, the exhaust valve 541 is opened to exhaust the gas in the jacking air bag 54, the top seat 52 is reset under the action of the supporting spring 53 to release the reagent bottles until the reagent bottles are completely immersed in the reagent tank 3, the exhaust valve 541 is closed after the reagent bottles stop descending, the second piston mechanism 431 is pulled to guide the gas to enter the second air cavity 432 from the outside through the third one-way valve 433, and then enter each supporting air bag 42 through the fourth one-way valve 434 and communicated pipelines to inflate the supporting air bags 42, when the supporting air bags 42 stretch until the gas is exhausted out of the first one-way valve 553 to jack the first piston mechanism 551, the supporting air bags 42 stop inflating the supporting air bags 42, are in a stretching state, enter the reagent tank 3 through the supporting holes 31 to support and protect the reagent bottles, avoid the reagent bottle to carry out the rigidity collision to the reagent groove, cushion the support to the reagent bottle, improve the safety of reagent bottle storage, attach lid 2 lid at 1 tops of box body, shutoff bulge loop 22 carries out the shutoff to reagent groove 3, further guarantees the shutoff of reagent bottle, reduces lid 2 to the impact collision of lid 1, and through two piston barrels of carrying the handle intercommunication, conveniently carry to the box body and carry and draw the removal, and guarantee to aerify efficiency.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.

Claims

1. A magnetic bead method tissue/cell DNA extraction kit is characterized in that: the box body (1) comprises a plurality of reagent tanks (3) for containing reagent bottles and a jacking device (5) which is arranged at the bottom of the box body and can jack the reagent bottles; the jacking device (5) comprises a fixed seat (51) fixed at the bottom of the box body (1) and used for supporting a reagent bottle, a top seat (52) capable of horizontally sliding and approaching to the fixed seat (51) to jack the bottom of the reagent bottle, a supporting spring (53) arranged on the fixed seat (51) and used for jacking and separating the top seat (52), a jacking air bag (54) used for jacking the top seat (52) to approach the supporting spring (53), and a first inflation unit (55) used for inflating the jacking air bag (54); an exhaust valve (541) which can be opened and closed is arranged on the pushing air bag (54); the first inflation unit (55) comprises a first piston mechanism (551) and a first air cavity (552) arranged at the air inlet end of the first piston mechanism (551); the first air cavity (552) is provided with a first one-way valve (553) for guiding air flow to enter the first air cavity (552) and a second one-way valve (554) for guiding air flow to enter the pushing air bag (54) from the first air cavity (552).

2. The kit for extracting DNA from tissue/cells by the magnetic bead method according to claim 1, wherein: a positioning device (4) for positioning and supporting the reagent bottle is arranged in the box body (1); the positioning device (4) comprises a plurality of air bag chambers (41) arranged around the reagent tank (3), supporting air bags (42) arranged in the air bag chambers (41), and second inflation units (43) communicated with the supporting air bags (42); a plurality of supporting holes (31) which are communicated with the air bag chamber (41) and the inner cavity of the reagent groove (3) and can be used for the supporting air bags (42) to pass through are formed in the side wall of the reagent groove (3); the second inflation unit (43) comprises a second piston mechanism (431), and a second air chamber (432) arranged at the air inlet end of the second piston mechanism (431); and a third one-way valve (433) for guiding outside air flow into the second air cavity (432) and a fourth one-way valve (434) for guiding air flow from the second air cavity (432) into the supporting air bag (42) are arranged on the second air cavity (432).

3. The kit for extracting tissue/cell DNA by the magnetic bead method according to claim 2, wherein: the air inlet end of the first one-way valve (553) is synchronously communicated with the plurality of supporting air bags (42) through a pipeline.

4. The kit for extracting DNA from tissue/cells by the magnetic bead method according to claim 2, wherein: the first piston mechanism (551) comprises two synchronously lifting piston cylinders (555) and a lifting handle (556) connected with handles of the two piston cylinders (555); the piston in the piston cylinder (555) can only slide in a reciprocating way in the piston cylinder (555); the second piston means (431) is of the same construction as the first piston means (551).

5. The kit for extracting DNA from tissue/cells by the magnetic bead method according to claim 1, wherein: the top of the fixed seat (51) is provided with a flexible gasket for supporting the reagent bottle.

6. The kit for extracting DNA from tissue/cells by the magnetic bead method according to claim 1, wherein: a plurality of supporting blocks (511) arranged at intervals are arranged on the fixed seat (51); a plurality of inserting blocks (521) capable of being inserted into the gaps of the supporting blocks (511) are arranged on the top seat (52); the supporting spring (53) is arranged in the gap between the two supporting blocks (511).

7. The kit for extracting DNA from tissue/cells by magnetic bead method according to claim 6, wherein: the top of the supporting block (511) is provided with a supporting surface inclining downwards along the direction close to the top seat (52).

8. The kit for extracting DNA from tissue/cells by magnetic bead method according to claim 6, wherein: the top of the inserting block (521) is provided with a supporting surface which obliquely supports the reagent bottle downwards along the direction close to the supporting spring (53).

9. The kit for extracting DNA from tissue/cells by the magnetic bead method according to claim 1, wherein: the top of the box body (1) is provided with a cover body (2) which can be detached to seal the opening of the box body (1); the box body (1) is provided with a plurality of threaded rods (11); a through hole (21) for the threaded rod (11) to pass through is arranged on the cover body (2); and the cover body (2) is provided with a flexible plugging convex ring (22) matched with the inner diameter of the reagent groove (3).