CN218507602U - Device for separating and enriching bovine serum albumin in emulsion wastewater - Google Patents

Abstract

The utility model relates to a device of bovine serum albumin in separation enrichment emulsion waste water belongs to bovine serum albumin recovery technical field in the emulsion waste water. The emulsion sewage pool is sequentially communicated with a sewage tank, an imprinting filter membrane filter, a water outlet header pipe, a filtrate tank and a filtrate outlet pipeline through pipelines; the blotting filter membrane filter comprises a plurality of independent filter units, a sewage main pipe is branched into a plurality of independent pipelines to be communicated with the independent filter units, each independent filter unit comprises a filter cartridge and a filter element, the filter cartridge is connected with the independent pipelines, the filter element is arranged inside the filter cartridge, the filter element comprises an inner layer metal net and an outer layer metal net and a blotting filter membrane arranged between the two layers of metal nets, and a rubber sealing gasket is arranged between the filter cartridge and the filter units. The utility model is specially designed for filtering and recovering bovine serum albumin, and aims at a blot filtering membrane, a filtering device with reasonable structure is designed, and the filtering device has good sealing performance and good filtering effect; the filter element can be directly and integrally drawn out from the filter cartridge, so that the filter is convenient to disassemble and maintain and replace; the filter screen can the backwash, is convenient for collect bovine serum albumin sample, life cycle is long.

Description

Device for separating and enriching bovine serum albumin in emulsion wastewater

Technical Field

The utility model relates to a device of bovine serum albumin in separation enrichment emulsion waste water especially relates to a device based on bovine serum albumin in temperature sensitive type blotting material specificity separation enrichment emulsion waste water, belongs to bovine serum albumin recovery technical field in the emulsion waste water.

Background

The molecular imprinting technique is a technique for preparing a polymer having a specific recognition function in the presence of a template molecule. Compared with natural recognition materials (such as antibodies), the molecularly imprinted polymer has the advantages of good stability, strong practicability, simple and convenient preparation and the like, and is widely applied to the fields of biosensors, artificial receptors, selective cell recognition, solid phase extraction, separation and enrichment, drug controlled release and the like. A number of researchers have successfully investigated small molecule templates of molecularly imprinted polymers of proteins, such as lysozyme (Lyz, M) _W 23.8 kDa), ribonuclease A (RNase, M) _W 13.7 kDa) and cytochrome C (Cyt C, M) _W 12.3kDa)。

The treatment of waste water of dairy products is mainly divided into physical, chemical and biological methods. Wherein the chemical method can completely remove the pollutants in the dairy wastewater. The biological method mainly comprises the following steps: activated sludge processes, aeration tanks, sequencing batch reactors, upflow anaerobic sludge beds and anaerobic filters, however, these processes require large area and long time in the treatment process and also produce large amounts of sludge. In addition, there are other treatment methods such as nanofiltration, reverse osmosis coagulation and flocculation, electrochemical treatment membrane bioreactors, and the like. These treatment techniques are intended to treat the emulsion waste water to treat the water quality and to recover and reuse the water which has reached the standard after the treatment of the pollutants contained in the waste water, but these treatment methods have ignored the recovery and utilization of some organic pollutants such as proteins contained in the emulsion waste water. Bovine serum albumin is an endogenous protein and has the advantages of no toxicity, no immunogenicity, good biocompatibility, low cost and the like. It has wide biomedical value, and has been applied in tumor diagnosis and treatment, fluorescence imaging, etc. Bovine serum albumin belongs to natural degradable macromolecular organisms and is a renewable resource, but no device report specially aiming at bovine serum albumin recovery exists at present.

Disclosure of Invention

The utility model discloses a remedy prior art not enough, provide a separation enrichment emulsion waste water in bovine serum albumin's device.

In order to achieve the purpose, the utility model is realized by adopting the following technical scheme:

a device for separating and enriching bovine serum albumin in emulsion wastewater comprises an emulsion sewage tank, wherein the emulsion sewage tank is sequentially communicated with a sewage tank, an imprinted filter membrane filter, a water outlet header pipe, a filtrate tank and a filtrate outlet pipeline through pipelines; a first liquid level control valve is arranged between the emulsion sewage pool and the sewage tank, a sewage main pipe, a sewage pump and a flow regulating valve are arranged between the sewage tank and the blotting filter membrane filter, a second liquid level control valve is arranged in the filtrate tank, and a filtrate pump is arranged on a filtrate outlet pipeline; the blotting filter membrane filter comprises a plurality of independent filter units, a sewage main pipe is branched into a plurality of independent pipelines to be communicated with the independent filter units, each independent filter unit comprises a filter cartridge and a filter element, the filter cartridges are connected with the independent pipelines, the filter elements are arranged inside the filter cartridges, each filter element comprises an inner layer of metal mesh and an outer layer of metal mesh, a blotting filter membrane is arranged between the two layers of metal meshes, and a rubber sealing gasket is arranged between each filter cartridge and each filter unit.

Preferably, the inner layer metal mesh and the outer layer metal mesh of the filter element are both porous cylinders, the edges of the inner layer metal mesh and the outer layer metal mesh and the upper layer of the imprinted filter membrane are turned outwards, the inner side of the rubber sealing gasket is provided with a groove, and the groove is sleeved on the edge turning-outwards structure.

Preferably, the filter cartridge is connected with the independent pipeline through a flange, and the diameter of the rubber gasket is between the inner diameter of the filter cartridge and the diameter of the flange plate.

Preferably, the inlet pipeline and the outlet pipeline of the independent filtering unit are both provided with sampling valves, and the inlet pipeline of the independent filtering unit is also provided with a pressure gauge.

Preferably, the front end of the independent filtering unit is connected with a cleaning pipeline and a cleaning valve.

Compared with the prior art, the utility model discloses an advantage lies in with positive effect:

the utility model is specially designed for filtering and recovering bovine serum albumin, and aims at a blot filtering membrane, a filtering device with reasonable structure is designed, and the filtering device has good sealing performance and good filtering effect; the filter element can be directly and integrally drawn out of the filter cylinder, so that the filter is convenient to disassemble and maintain and replace; the filter screen can be backwashed, so that a bovine serum albumin sample can be conveniently collected, and the service cycle is long; and the sampling and monitoring are convenient in real time.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

FIG. 2 is a schematic diagram of the structure of the blotting filter device.

FIG. 3 is a schematic view of a filter unit

The various reference numbers are: 1 sewage tank, 2 blotting filter membrane filters, 3 water outlet header pipes, 4 filtrate tanks, 5 filtrate outlet pipelines, 6 first liquid level control valves, 7 sewage header pipes, 8 sewage pumps, 9 flow regulating valves, 10 second liquid level control valves, 11 filtrate pumps, 12 metal meshes, 13 sampling valves and 14 pressure meters.

Detailed Description

In order to make the above objects, features and advantages of the present invention more clearly understood, the present invention will be further described with reference to the accompanying drawings and examples. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and therefore the present invention is not limited to the limitations of the specific embodiments of the present disclosure.

Example 1

The accompanying drawings illustrate a specific embodiment of the present invention, as shown in fig. 1-3.

The device for separating and enriching bovine serum albumin in emulsion wastewater provided by the embodiment comprises an emulsion sewage pool, sewage in the emulsion sewage pool enters a sewage tank 1 through a pipeline, the liquid level of the sewage tank 1 is controlled through a liquid level meter on the sewage tank 1 and a valve (a first liquid level control valve 6) on the pipeline, and an emptying valve is arranged at the upper part of the sewage tank 1. The outlet of the emulsion sewage pool can be designed to be higher than the inlet of the sewage tank 1, so that sewage enters the sewage tank 1 under the action of gravity, or a corresponding pump is arranged to provide power, and the outlet of the emulsion sewage pool can be provided with a filter screen to reduce particle impurities from entering the sewage tank 1 and subsequent parts. The outlet of the sewage tank 1 is provided with a sewage pump 8, and two sewage pumps can be arranged, one sewage pump is standby and convenient to overhaul. The other end of the sewage pump 8 is provided with a sewage main pipe 7, and the sewage main pipe 7 is provided with a corresponding flow regulating valve 9 and a pressure gauge of the main pipe. The sewage main pipe 7 is divided into a plurality of branch pipelines through a connector (customized as required), each branch pipeline is communicated to an independent blotting membrane filter 2, each branch pipeline of an outlet of the blotting membrane filter 2 is gathered to the water outlet main pipe 3 through connector connection, the water outlet main pipe 3 is connected with a filtrate tank 4 which is used for containing filtrate after filtration, the filtrate tank 4 is controlled by the second liquid level control valve 10 and then communicated with a filtrate outlet pipeline 5, and a corresponding filtrate pump 11 is arranged on the filtrate outlet pipeline 5.

The independent filter unit comprises a filter cartridge and a filter element, the filter cartridge is connected with an independent pipeline, the filter element is arranged inside the filter cartridge, the filter element comprises an inner layer of metal mesh 12 and an outer layer of metal mesh 12 and a print filter membrane arranged between the two layers of metal mesh 12, and a rubber sealing gasket is arranged between the filter cartridge and the filter element. The inner and outer layers of metal nets of the filter element are thin-walled porous cylinders, the inner and outer layers of metal nets 12 and the upper layer of the imprinted filter membrane are turned outwards, the inner side of the rubber sealing gasket is provided with a groove, and the groove is sleeved on the turned-outwards structure. The filter cartridge is connected with the independent pipeline through a flange, and the diameter of the rubber gasket is between the inner diameter of the filter cartridge and the diameter of the flange disc. The inlet pipeline and the outlet pipeline of the independent filtering unit are both provided with sampling valves 13, and the inlet pipeline of the independent filtering unit is also provided with a pressure gauge 14. The front end of the independent filtering unit is connected with a cleaning pipeline and a cleaning valve.

Specifically, the sewage is delivered to a sewage header pipe 7, the water in the sewage header pipe 7 is divided into a plurality of independent pipelines to enter the independent blotting membrane filters 2 to realize the separation and enrichment of bovine serum albumin, and the details are explained by using one independent blotting membrane filter 2. The sewage of house steward at first gets into independent branch pipe, the sewage of branch pipe passes through the pipeline and gets into blotting membrane filter 2, water after adsorbing filtration through the blotting membrane flows out from the branch pipe lower extreme, gather water outlet manifold 3, the water of house steward passes through filtrate pump 11 and carries the external world, realize the follow-up industry of water and retrieve the circulation and use, the bovine serum albumin of adsorption enrichment on blotting membrane filter 2 elutes or dismantles the back with acid aqueous solution through rinsing mouthful with acid aqueous solution and elutes, the bovine serum albumin that elutes is collected for subsequent use. A valve is arranged in front of each independent water inlet pipeline, so that the blotting membrane filter 2 can be conveniently overhauled when being blocked or damaged. The valve is connected with a pipeline through a flange, a sealing gasket is added between the flange and the flange, and then the valve is fixed through bolts. The sampling valves are arranged at the water inlet pipe and the water outlet pipe on each independent pipeline, the sampling valves are convenient for collecting water samples at the water inlet pipe and the water outlet pipe, the concentration of bovine serum albumin in the water solution is detected before and after separation, and the sampling valves can be used as a vent and a drain outlet. Each independent water inlet pipeline is provided with a pressure gauge 14, the pressure gauge 14 detects the pressure change of each pipeline, the blockage or damage condition of the blotting filter membrane filter 2 is monitored at any time, and if the pressure is increased, the blotting filter membrane filter 2 is seriously blocked and needs to be cleaned or replaced. Each independent blotting membrane filter consists of two parts, one is a filtering unit and the other is a filtrate cylinder. The filter unit mainly comprises three parts, wherein the first layer is a corrosion-resistant metal net, the second layer is a print filter membrane with a specific adsorption effect, the third layer is also a corrosion-resistant metal net, the three layers form a core part filter element of the print membrane filter, the whole structure of the filter unit is cylindrical, the periphery and the bottom of the filter unit are both provided with structures with uniformly and densely distributed holes, the opening of the circular filter element cylinder is outwards turned out to form a circular outer edge, and the circular outer edge is covered by the metal sheet to form a flat circular opening at the upper end. The outer edge of the metal filter element is coated with a rubber sealing gasket which is corrosion-resistant, high-pressure-resistant and high-temperature-resistant, a groove is formed in the inner side of the sealing ring, the sealing ring is just clamped on the outer edge of the metal filter element, the outer diameter of the sealing ring is larger than the inner diameter of the imprinted membrane filter cylinder, so that the metal filter element can be suspended on the upper edge of the filter cylinder, the filter element is integrally suspended, and the filter liquor can flow out conveniently. The filter element with the sealing ring is placed on the filter cylinder, the bolt holes are formed in the outer edge of the filter cylinder, the sealing gasket is added to the upper portion of the filter cylinder, and the upper pipeline is perfectly connected with the lower filter cylinder through the bolts. In addition, the front part of each independent blotting filter membrane filter 2 is provided with a cleaning pipeline (independently arranged), and the pipeline is provided with a valve, so that the blotting filter can be cleaned conveniently.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in other forms, and any person skilled in the art may use the above-mentioned technical contents to change or modify the equivalent embodiment into equivalent changes and apply to other fields, but any simple modification, equivalent change and modification made to the above embodiments according to the technical matters of the present invention will still fall within the protection scope of the technical solution of the present invention.

Claims (5)

1. The device for separating and enriching bovine serum albumin in emulsion wastewater is characterized by comprising an emulsion sewage tank, wherein the emulsion sewage tank is sequentially communicated with a sewage tank, a blotting filter membrane filter, a water outlet header pipe, a filtrate tank and a filtrate outlet pipeline through pipelines; a first liquid level control valve is arranged between the emulsion sewage pool and the sewage tank, a sewage main pipe, a sewage pump and a flow regulating valve are arranged between the sewage tank and the blotting filter membrane filter, a second liquid level control valve is arranged in the filtrate tank, and a filtrate pump is arranged on a filtrate outlet pipeline; the blotting filter membrane filter comprises a plurality of independent filter units, a sewage main pipe is branched into a plurality of independent pipelines to be communicated with the independent filter units, each independent filter unit comprises a filter cartridge and a filter element, the filter cartridges are connected with the independent pipelines, the filter elements are arranged inside the filter cartridges, each filter element comprises an inner layer of metal mesh and an outer layer of metal mesh, a blotting filter membrane is arranged between the two layers of metal meshes, and a rubber sealing gasket is arranged between each filter cartridge and each filter element.

2. The device for separating and enriching bovine serum albumin in emulsion wastewater according to claim 1, wherein the inner and outer metal nets of the filter element are porous cylinders, the edges of the inner and outer metal nets and the upper layer of the blotting filter membrane are turned outwards, and the inner side of the rubber sealing gasket is provided with a groove which is sleeved on the edge turning-outwards structure.

3. The apparatus for separating bovine serum albumin enriched in emulsion wastewater according to claim 2, wherein the filter cartridge is connected with the independent pipeline through a flange, and the diameter of the rubber gasket is between the inner diameter of the filter cartridge and the diameter of the flange.

4. The apparatus for separating bovine serum albumin from emulsion-enriched wastewater according to claim 3, wherein the inlet pipeline and the outlet pipeline of the independent filtration unit are both provided with sampling valves, and the inlet pipeline of the independent filtration unit is also provided with pressure gauges.

5. The apparatus of claim 4, wherein the front end of the independent filtering unit is connected to a cleaning pipeline and a cleaning valve.

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