CN218491694U

CN218491694U - Culture apparatus of survey bacterium concentration

Info

Publication number: CN218491694U
Application number: CN202221215689.6U
Authority: CN
Inventors: 潘海婷; 王利
Original assignee: Inner Mongolia Medical University
Current assignee: Inner Mongolia Medical University
Priority date: 2022-05-20
Filing date: 2022-05-20
Publication date: 2023-02-17
Anticipated expiration: 2032-05-20

Abstract

The utility model discloses a culture device for measuring the concentration of bacteria, which comprises a constant-temperature culture shaking table, a triangular flask and an ultraviolet spectrophotometer, wherein the triangular flask is arranged in the constant-temperature culture shaking table for bacterial culture, a liquid taking hole is arranged on the side wall of the triangular flask, a liquid taking pipe is arranged in the liquid taking hole, the outer end of the liquid taking pipe extends out of the constant-temperature culture shaking table and is connected with a liquid taking pump, the outlet of the liquid taking pump is connected with a guide pipe, and the guide pipe is communicated with a sample pool of the ultraviolet spectrophotometer; and directly taking the bacterial liquid from the triangular flask by the liquid taking pump, and then introducing the bacterial liquid into an ultraviolet spectrophotometer in real time for monitoring and measuring. The utility model discloses in, combine the characteristic of constant temperature cultivation shaking table and ultraviolet spectrophotometer, can carry out the concentration of real-time supervision fungus liquid at the cultivation bacterial in-process, need not take out the fungus liquid and carry out concentration measurement, very big data measurement who has made things convenient for in the medical test has guaranteed measured data's accuracy to and the security of medical experiment.

Description

Culture apparatus of survey bacterium concentration

Technical Field

The utility model belongs to the technical field of the medical science clinical science and specifically relates to a survey culture apparatus of bacterium concentration.

Background

In the process of a laboratory bacterial culture experiment, the concentration of bacteria is often required to be quantitatively measured, the conventional method for measuring the concentration of bacteria comprises a Mach turbidimetry method, an ultraviolet spectrophotometry and the like, the Mach turbidimetry method is often used for visually measuring the approximate concentration of a bacterial liquid, and the ultraviolet spectrophotometer 3 can be used for accurately measuring the bacterial liquid, but cannot be used for monitoring and measuring in real time.

However, the ultraviolet spectrophotometer 3 cannot measure the concentration of bacteria in real time by monitoring the progress of the culture of bacteria.

SUMMERY OF THE UTILITY MODEL

To the problem that prior art exists, the utility model aims to provide a structure is through improving, be convenient for real-time measuring's survey bacteria concentration's culture apparatus.

In order to achieve the purpose, the utility model relates to a culture device for measuring the concentration of bacteria, which comprises a constant temperature culture shaking table, a triangular flask and an ultraviolet spectrophotometer, wherein the triangular flask is arranged in the constant temperature culture shaking table for bacteria culture, a liquid taking hole is arranged on the side wall of the triangular flask, a liquid taking pipe is arranged in the liquid taking hole, the outer end of the liquid taking pipe extends out of the constant temperature culture shaking table and then is connected with a liquid taking pump, the outlet of the liquid taking pump is connected with a conduit, and the conduit is communicated with a sample pool of the ultraviolet spectrophotometer; and directly taking the bacterial liquid from the triangular flask by the liquid taking pump, and then introducing the bacterial liquid into an ultraviolet spectrophotometer in real time for monitoring and measuring.

Furthermore, a sealing ring is arranged on the liquid taking hole, and the liquid taking pipe is inserted in the sealing ring to be fixedly installed.

Further, the sealing ring comprises a fixing ring and a movable ring, and the movable ring is sleeved inside the fixing ring.

Further, the fixed ring is fixedly installed on the side wall of the triangular flask, the fixed ring is of a hollow structure, and the movable ring is movably arranged in the fixed ring.

Further, a sealing ring is arranged between the inner wall of the fixing ring and the outer wall of the movable ring.

Further, the sealing ring is arranged on the edge of the movable ring.

Furthermore, the middle part of the movable ring is provided with a through hole for inserting the liquid taking pipe.

Further, the movable ring and the sealing ring are integrally made of sealing rubber.

Furthermore, a plurality of small holes are arranged on part of the pipe wall of the liquid taking pipe in the triangular flask.

Further, the inlet and the outlet of the liquid taking pump are both provided with check valves.

The utility model discloses in, combine constant temperature to cultivate shaking table and ultraviolet spectrophotometer's characteristic, can carry out the concentration of real-time supervision fungus liquid at the cultivation bacterium in-process, need not take out the fungus liquid and carry out the concentration measurement, very big data measurement who has made things convenient for in the medical experiment has guaranteed measured data's accuracy to and the security of medical experiment.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of a seal ring structure;

FIG. 3 is a first schematic view illustrating a usage state of the seal ring;

fig. 4 is a schematic view of a second usage state of the seal ring.

Detailed Description

The technical solution of the present invention will be described clearly and completely with reference to the accompanying drawings, and obviously, the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

The following detailed description of the embodiments of the present invention will be made with reference to the accompanying drawings. It should be understood that the description herein is provided for illustration and explanation of the invention and is not intended to limit the invention.

As shown in FIGS. 1 to 4, the utility model relates to a culture apparatus for determining bacterial concentration, which comprises a constant temperature culture shaking table 1, a triangular flask 2 and an ultraviolet spectrophotometer 3.

The constant temperature culture shaking table 1 is a multipurpose biochemical device, is laboratory equipment indispensable for precise culture and preparation in scientific research, education and production departments of plants, organisms, microorganisms, heredity, viruses, environmental protection, medicine and the like, and is suitable for shaking culture of various liquid and solid compounds such as organisms, biochemistry, cells, strains and the like in scientific research departments of universities and colleges, oil chemical industry, health epidemic prevention, environmental monitoring and the like. In the utility model, the constant temperature shaking table 1 is a table with the devices in the prior art.

The ultraviolet spectrophotometer 3 can carry out quantitative measurement (standard curve measurement, direct concentration reading can be carried out on substances); the OD value is directly measured (direct reading of absorbance, transmittance, energy and the like); kinetic testing (measuring the change in OD of the concentration of the substance over time); spectral scanning (full-wave-band scanning can be carried out on a certain substance, the characteristic wavelength of the substance is analyzed, and the error of the experimental process is judged); an instrument for multi-wavelength testing (which can simultaneously test a substance at multiple wavelengths and analyze the relevant characteristics of the substance); besides the functions, the device can be used for DNA/protein test, total phosphorus and total nitrogen test, heavy metal test, pesticide residue test, food safety detection, thermal power generation metal ion test and the like, and is widely applied to various fields of food, medicine, electric power, biological research, teaching and scientific research, chemical industry, quality supervision, water quality environmental protection, commodity inspection and the like. Such as UV-1500PC type ultraviolet-visible spectrophotometer, wavelength range: 190nm-1100nm, which can meet the test of different substances. In the utility model, the ultraviolet spectrophotometer 3 adopts the equipment in the prior art can.

The utility model discloses in, the triangular flask 2 sets up carries out the bacterial culture in constant temperature cultivates shaking table 1, and the fungus liquid in the triangular flask 2 needs to be measured in leading-in ultraviolet spectrophotometer 3.

Liquid taking holes 12 are formed in the side wall of the triangular bottle 2, sealing rings 7 are arranged on the liquid taking holes 12, and liquid taking pipes 6 are inserted into the sealing rings 7 to be fixedly installed. The sealing ring 7 is a fixed mounting part for the extractor tube 6.

Simultaneously, because during the bacteria cultivation is carried out to triangular flask 2 in constant temperature cultivation shaking table 1, the active state can appear in triangular flask 2, in order to guarantee external device's stability, need guarantee that liquid taking pipe 6 is in stable state, so sealing ring 7 need possess simultaneously can the motion and sealed dual function.

Sealing ring 7 is including solid fixed ring 8 and activity ring 9, and solid fixed ring 8 adopts hard material to make, and activity ring 9 adopts the sealing rubber material to make, and solid fixed ring 8 fixed mounting is on the lateral wall of triangular flask 2, and solid fixed ring 8 is hollow structure, and its internalization is provided with activity ring 9.

A sealing ring 11 is arranged between the inner wall of the fixed ring 8 and the outer wall of the movable ring 9. The sealing ring 11 ensures effective sealing between the fixed ring 8 and the movable ring 9, preventing liquid leakage. The side wall of the inner ring of the fixing ring 8 is provided with a limiting ring 10, the sealing ring 11 is arranged at the edge of the movable ring 9, the sealing ring 11 is simultaneously used as one of movable limiting parts of the movable ring 9, the limiting ring 10 and the sealing ring 11 are integrally combined into the movable limiting part of the movable ring 9, and the movable ring 9 is limited to move within a certain range.

The middle part of activity ring 9 is provided with the through-hole 13 that is used for alternate liquid taking pipe 6, and the diameter of through-hole 13 slightly is lighter than the diameter of liquid taking pipe 6. The movable ring 9 is also made of sealing rubber and has certain elasticity, and after the liquid taking pipe 6 is inserted into the through hole 13, the liquid taking pipe 6 is fixedly connected with the movable ring 9 into a whole. Because be in movable state between activity ring 9 and the solid fixed ring 8, when the triangular flask 2 drove solid fixed ring 8 and moves together, can guarantee that liquid taking pipe 6 and activity ring 9 keep relative quiescent condition. As shown in FIG. 3 and FIG. 4, the two extreme positions of the fixed ring 8 and the movable ring 9 are shown in the vertical direction when the triangular flask 2 is moved in the constant temperature incubator 1. As can be seen from fig. 3 and 4, although the position of the triangular flask 2 is shifted during the movement, the fixed ring 8 and the movable ring 9 are still in a sealed state, and the evidence liquid tube 6 and the movable ring 9 can also be kept in a relatively stationary state.

In this embodiment, the movable ring 9 and the seal ring 11 are made of sealing rubber integrally. In order to obtain the bacterial liquid in each region of the triangular flask 2 and ensure the consistency of the concentration of the obtained bacterial liquid, the liquid taking pipe 6 positioned in the triangular flask 2 can be properly lengthened, and the pipe wall of the extension part is provided with a plurality of small holes.

The utility model discloses in, get liquid pump 4 adopt among the prior art equipment can, get all to be provided with the check valve in the exit of liquid pump 4.

When the device works, a sealing ring 7 is arranged in the liquid taking hole 12, a liquid taking pipe 6 is arranged in the sealing ring 7, the outer end of the liquid taking pipe 6 extends out of the constant-temperature culture shaking table 1 and then is connected with a liquid taking pump 4, a guide pipe 5 is connected to the outlet of the liquid taking pump 4, and the guide pipe 5 is communicated with a sample pool of the ultraviolet spectrophotometer 3; the liquid taking pump 4 works in real time according to the requirement set by the work, and directly takes a certain amount of bacteria liquid from the triangular flask 2 in a set time interval, and then introduces the bacteria liquid into the ultraviolet spectrophotometer 3 in real time for monitoring and determination.

Claims

1. A culture device for determining the concentration of bacteria is characterized by comprising a constant-temperature culture shaking table, a triangular flask and an ultraviolet spectrophotometer, wherein the triangular flask is arranged in the constant-temperature culture shaking table for bacterial culture, a liquid taking hole is formed in the side wall of the triangular flask, a liquid taking pipe is arranged in the liquid taking hole, the outer end of the liquid taking pipe extends out of the constant-temperature culture shaking table and then is connected with a liquid taking pump, the outlet of the liquid taking pump is connected with a guide pipe, and the guide pipe is communicated with a sample pool of the ultraviolet spectrophotometer; and directly taking the bacterial liquid from the triangular flask by the liquid taking pump, and then introducing the bacterial liquid into an ultraviolet spectrophotometer in real time for monitoring and measuring.

2. The culture apparatus for measuring the concentration of bacteria according to claim 1, wherein the liquid extraction hole is provided with a sealing ring, and the liquid extraction pipe is fixedly installed by being inserted into the sealing ring.

3. The culture apparatus for measuring a concentration of bacteria according to claim 2, wherein the sealing ring comprises a fixed ring and a movable ring, and the movable ring is fitted inside the fixed ring.

4. The culture apparatus for measuring the concentration of bacteria according to claim 3, wherein the fixed ring is fixedly installed on the side wall of the triangular flask, the fixed ring has a hollow structure, and the movable ring is movably installed therein.

5. The culture device for measuring the concentration of bacteria according to claim 3, wherein a sealing ring is provided between an inner wall of the fixed ring and an outer wall of the movable ring.

6. The culture device for measuring the concentration of bacteria according to claim 5, wherein the sealing ring is disposed at the edge of the movable ring.

7. The culture apparatus for measuring a concentration of bacteria according to claim 3, wherein a through hole for inserting the liquid extraction pipe is provided at a middle portion of the movable ring.

8. The culture device for measuring the concentration of bacteria according to claim 5, wherein the movable ring and the sealing ring are integrally formed of a sealing rubber.

9. The culture device for measuring the concentration of bacteria according to claim 1, wherein the liquid extraction tube is provided with a plurality of small holes on a part of the tube wall in the triangular flask.

10. The culture apparatus for measuring the concentration of bacteria according to claim 1, wherein check valves are provided at the inlet and outlet of the liquid-extracting pump.