CN218458709U

CN218458709U - Protein purification chromatography equipment

Info

Publication number: CN218458709U
Application number: CN202222576661.1U
Authority: CN
Inventors: 高智星; 胡俊华; 侯方政; 温剑阳
Original assignee: Mobao Xiamen Biotechnology Co ltd
Current assignee: Mobao Xiamen Biotechnology Co ltd
Priority date: 2022-09-28
Filing date: 2022-09-28
Publication date: 2023-02-10
Anticipated expiration: 2032-09-28

Abstract

The utility model is suitable for the technical field of biological preparation, and provides a protein purification chromatography device, which comprises a transverse plate, an installation mechanism and a clamping mechanism; the vertical fixedly connected with riser of the other end at diaphragm top, the vertical fixedly connected with fixed box of the other end at diaphragm top, the vertical fixedly connected with install bin of one side of fixed box. This protein purification chromatography equipment, when needing to install the chromatography column, through placing the chromatography column in the protective housing inner chamber, the limiting plate rotates to the horizontality, the chromatography column is connected with pan feeding pipe and conveyer pipe, the pan feeding pipe is carried the sample to the chromatography column in, the chromatography column carries out the stratification analysis, carry the sample to detect in the protein analysis equipment through the conveyer pipe, the conveyer pipe is carried liquid to the holding vessel of difference by the branch material pipe, make qualified and unqualified sample separately deposit, make things convenient for follow-up unified ejection of compact, protect the chromatography column, the chromatography column of easy to assemble simultaneously is dismantled the maintenance with follow-up operating personnel to the chromatography column, excellent in use effect.

Description

Protein purification chromatography equipment

Technical Field

The utility model belongs to the technical field of biological preparation, especially, relate to a protein purification chromatography equipment.

Background

The protein purification and chromatography equipment is the most central equipment in the development and production process of protein medicines, and is used for removing impurities in the cell culture process and extracting a pure product which can be used for injection, namely the protein medicines.

When to protein purification chromatography, generally mainly carry out the purification chromatography of protein through the chromatographic column, when using the chromatographic column, it is generally directly exposed in external environment, when the chromatography, if receive unexpected condition striking and rock, can lead to the chromatographic column to damage, lead to inside protein to reveal, influence the chromatography and imitate and strain, the result of use is not good, and the device is installed simultaneously, generally directly places in the assigned position or through the bolt installation, and the fixed effect of installation is not good.

SUMMERY OF THE UTILITY MODEL

The utility model provides a protein purification chromatography equipment aims at solving when the chromatography, if receive the unexpected condition striking and rock, can lead to the chromatography column to damage, leads to inside protein to reveal, influences the chromatography and imitates and strain, and the result of use is not good, and the device is installed when the installation simultaneously, generally directly places in assigned position or through the bolt installation, installs the problem that fixed effect is not good.

The utility model is realized in such a way, and the protein purification chromatography device comprises a transverse plate, a mounting mechanism and a clamping mechanism;

the device comprises a transverse plate, a vertical plate, a fixing box, a conveying pipe, a protein analysis device, a protective box, a material distribution pipe, a chromatography column and a chromatography column, wherein the other end of the top of the transverse plate is vertically and fixedly connected with the vertical plate, the other end of the top of the transverse plate is vertically and fixedly connected with the fixing box, one side of the fixing box is vertically and fixedly connected with the mounting box, the inner cavity of the mounting box is vertically and fixedly connected with the protein analysis device, one side of the mounting box is vertically and fixedly connected with the protective box, the top of the protective box is vertically and communicated with the material distribution pipe, the bottom of the inner cavity of the protective box is communicated with the conveying pipe, the bottom of the conveying pipe penetrates to the top of the inner cavity of the fixing box through the protein analysis device, the surface of the conveying pipe is vertically and communicated with the inner cavity of the fixing box, and the bottom of the protective box and the top of the material distribution pipe are vertically and detachably provided with the chromatography column;

the mounting mechanism comprises two fixed columns, one ends of the fixed columns are rotatably provided with limiting plates, and the inner side surfaces of the limiting plates are in contact with the surfaces of the chromatographic columns;

fixture detachably set up in the opposite side of riser, fixture includes the mounting panel, the opposite side of mounting panel rotationally transversely is provided with the axis of rotation, the other end fixedly connected with connecting plate of axis of rotation, the front end and the equal sliding connection in rear end of connecting plate opposite side have the grip block.

Preferably, a through hole is vertically formed in one side of the protection box, and a detachable and vertically-arranged disassembling plate capable of plugging the through hole is detachably arranged on one side of the protection box.

Preferably, the equal vertical bin that is connected with in both sides of fixed case inner chamber, it is a plurality of divide the material pipe to run through respectively to corresponding the bin inner chamber, the vertical intercommunication in bottom of bin inner chamber is provided with the discharging pipe, the bottom of discharging pipe run through to the bottom of fixed case.

Preferably, the top and the bottom of mounting panel transversely run through with the equal threaded connection ground of bottom and be provided with connecting bolt, the top and the bottom of riser opposite side with the both ends of diaphragm bottom all seted up with the screw hole of connecting bolt looks adaptation.

Preferably, a connector is formed in one end of the fixing column in an inwards recessed mode, a rotating rod is rotatably and transversely arranged in an inner cavity of the connector, and one end of the rotating rod extends to the outside of the connector and is fixedly connected with the limiting plate.

Preferably, the front end and the rear end of the other side of the connecting plate are fixedly connected with connecting blocks transversely, two bidirectional threaded rods are rotatably arranged between the connecting blocks, threads at two ends of the surfaces of the bidirectional threaded rods are opposite, thread blocks are connected at two ends of the surfaces of the bidirectional threaded rods in a threaded manner, and the clamping plate is fixedly connected with the corresponding thread blocks.

Advantageous effects

Compared with the prior art, the beneficial effects of the utility model are that: the utility model discloses a protein purification chromatography equipment, when the chromatography column needs to be installed, through placing the chromatography column in the protective housing inner chamber, the limiting plate rotates to the horizontality, thereby block the chromatography column, the chromatography column is connected with pan feeding pipe and conveyer pipe, the pan feeding pipe is carried the sample to the chromatography column in, carry out the stratification analysis of protein through the chromatography column, carry the sample to the protein analysis equipment in through the conveyer pipe and detect, when making the conveyer pipe carry liquid to the storage tank of difference by the branch material pipe according to testing result, make qualified and unqualified sample separately deposit, make things convenient for follow-up unified ejection of compact, protect the chromatography column, the maintenance is dismantled to the chromatography column with follow-up operating personnel to the chromatography column of easy to assemble simultaneously, the result of use is better.

Drawings

FIG. 1 is a front view of the present invention showing a cross-sectional structure;

FIG. 2 is a schematic top view of the structure of the clamping plate and the bidirectional threaded rod of the present invention;

fig. 3 is a schematic top sectional view of the transfer rod and the fixed column structure of the present invention.

In the figure: 1-transverse plate, 2-vertical plate, 3-fixed box, 4-installation box, 5-protection box, 6-feeding pipe, 7-conveying pipe, 8-chromatographic column, 9-protein analysis equipment, 10-storage box, 11-material distributing pipe, 12-discharging pipe, 13-installation plate, 14-rotating shaft, 15-connecting plate, 16-connecting block, 17-bidirectional threaded rod, 18-threaded block, 19-clamping plate, 20-fixed column, 21-rotating rod, 22-limiting plate and 23-locating plate.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Referring to fig. 1-3, the present invention provides a technical solution: a protein purification chromatography device comprises a transverse plate 1, an installation mechanism and a clamping mechanism; the vertical fixedly connected with riser 2 of the other end at diaphragm 1 top, the vertical fixedly connected with fixed box 3 of the other end at diaphragm 1 top, the vertical fixedly connected with install bin 4 of one side of fixed box 3, the vertical fixedly connected with albumen analytical equipment 9 of 4 inner chambers of install bin, the vertical fixedly connected with protective box 5 of one side of install bin 4, the vertical intercommunication in top of protective box 5 is provided with pan feeding pipe 6, the bottom intercommunication of protective box 5 inner chamber is provided with conveyer pipe 7, the bottom of conveyer pipe 7 by the bottom warp of install bin 4 albumen analytical equipment 9 runs through to the top of fixed box 3 inner chamber, the surface of conveyer pipe 7 just is located the vertical intercommunication of fixed box 3 inner chambers is provided with a plurality of branch material pipes 11, the bottom of protective box 5 with just be located between the top of pan feeding pipe 6 the vertical chromatographic column 8 that is provided with of protective box 5 inner chamber detachably.

The bottom of riser 2 and diaphragm 1's top fixed connection, thereby guarantee riser 2's stability, the bottom of fixed box 3 is provided with the supporting leg, the bottom of supporting leg and diaphragm 1's top fixed connection, there is certain interval through the supporting leg messenger between fixed box 3 and the diaphragm 1, guarantee subsequent flowing back effect, mode fixed connection such as opposite side and one side accessible welding or bolt of fixed box 3 of install bin 4, the fixed mode does not specifically prescribe a limit to, the opposite side of guard box 5 and one side fixed connection of install bin 4 simultaneously, guarantee the stability of guard box 5, the bottom of install bin 4 is provided with the top fixed connection of supporting leg and diaphragm 1 equally.

Protein analysis equipment 9 is current device, it can be the ultraviolet detector, after passing through chromatographic column 8 with protein purification chromatography, liquid accessible conveyer pipe 7 is carried to protein analysis equipment 9 after the purification, carry out corresponding detection to it through protein analysis equipment 9, the protein sample after the detection is accomplished passes through conveyer pipe 7 and carries to branch material pipe 11 in, the protein discharge after will detecting the completion by branch material pipe 11 is collected to the assigned position, chromatographic column 8 is ripe current device, therefore do not do specific explanation, install bin 4 inner chamber is provided with the water pump, conveyer pipe 7 guarantees through the water pump that it can be to dividing in the material pipe 11 liquid conveying.

Installation mechanism includes two fixed columns 20, the one end of fixed column 20 rotationally is provided with limiting plate 22, limiting plate 22 the medial surface with the surface of chromatography column 8 contacts, the connector has been seted up to the one end of fixed column 20 inwards caves in, the connector inner chamber rotationally transversely is provided with dwang 21, the one end of dwang 21 extends to the outside of connector and with limiting plate 22 fixed connection.

One end of the rotating rod 21 is rotatably connected with the other end of the inner wall surface of the connecting port through a damping bearing, so that the rotating stability of the rotating rod 21 is ensured, meanwhile, one end of the rotating rod 21 is fixedly connected with the surface of the limiting plate 22, so that the limiting plate 22 is driven to rotate to be vertical or horizontal when the rotating rod 21 rotates, the test tube is in contact with or is not in contact with the test tube, the stability when the test tube is communicated with the feeding tube 6 and the conveying tube 7 is ensured,

only carry out preliminary spacing to the test tube, the top and the bottom of 5 inner chambers of protective housing all are provided with pipe connection, can make the bottom of pan feeding pipe 6 and the top of chromatography post 8 be connected through pipe connection, the top of conveyer pipe 7 is connected with the bottom of chromatography post 8, the surface of dwang 21 is encircleed the reduction and is provided with a plurality of spacing mouths, the internal face of connector encircles the interval and is provided with a plurality of elastic fixture blocks with connector looks adaptation, thereby can carry out preliminary spacing its rotation of avoiding to dwang 21.

The rear end in the inner cavity of the protective box 5 can be transversely provided with a positioning plate 23 to avoid the inclination of the chromatographic column 8.

Fixture detachably set up in the opposite side of riser 2, fixture includes mounting panel 13, mounting panel 13's opposite side rotationally transversely is provided with axis of rotation 14, the other end fixedly connected with connecting plate 15 of axis of rotation 14, the front end and the equal sliding connection in rear end of connecting plate 15 opposite side have grip block 19.

When needs are installed the device to the assigned position, thereby can install the installation demand to the bottom of diaphragm 1 or riser 2's opposite side and satisfy multiple difference according to the user demand, the rotation through axis of rotation 14 drives the position that clamping plate 19 was adjusted to connecting plate 15, can carry out adaptability according to concrete installation environment equally and adjust, two clamping plates 19 can be close to each other or keep away from each other to change the interval between two clamping plates 19, satisfy the centre gripping to the assigned position, thereby guarantee fixed mounting.

The surface threaded connection ground of connecting plate 15 transversely runs through there is spacing bolt, and the opposite side of mounting panel 13 encircles the interval and is provided with a plurality of screw holes with spacing bolt looks adaptation to can make its and screw hole threaded connection through rotating spacing bolt, fix the position of connecting plate 15, guarantee that connecting plate 15 is stable.

Furthermore, one side of the protection box 5 is vertically provided with a through hole, and one side of the protection box 5 is detachably and vertically provided with a disassembling plate capable of plugging the through hole.

In this embodiment, dismantle board accessible bolt or other modes and can dismantle with the surface of protective housing 5 and be connected, can pass through the shutoff of mouthful or cancel the shutoff through dismantling the board to the accessible passes through mouthful to dismantle the change or the installation to the chromatography column 8 that is arranged in the inner chamber of protective housing 5, satisfies the user demand.

Further, the equal vertical bin 10 that is connected with in both sides of 3 inner chambers of fixed case, it is a plurality of branch material pipe 11 runs through to corresponding respectively the bin 10 inner chamber, the vertical intercommunication in bottom of bin 10 inner chamber is provided with discharging pipe 12, the bottom of discharging pipe 12 run through to the bottom of fixed case 3.

In this embodiment, the bottom of bin 10 and the bottom fixed connection of 3 inner chambers of fixed case, thereby guarantee bin 10's stability, bin 10 can be provided with two simultaneously, two bin 10 are used for storing respectively and detect qualified sample and unqualified sample, thereby make things convenient for follow-up ejection of compact according to user demand selectivity, all be provided with the solenoid valve on branch material pipe 11 with discharging pipe 12, the solenoid valve is controlled through external control module, for ripe prior art, the bottom of two discharging pipe 12 is external infusion pipeline respectively simultaneously, sample with in the bin 10 inner chamber is discharged to the assigned position.

Further, the top and the equal threaded connection ground of bottom of mounting panel 13 transversely run through and are provided with connecting bolt, the top and the bottom of 2 opposite sides of riser and the both ends of diaphragm 1 bottom all seted up with the screw hole of connecting bolt looks adaptation.

In this embodiment, when the mounting plate 13 needs to be mounted to the other side of the vertical plate 2 or the bottom of the horizontal plate 1 according to the use requirement, the position of the mounting plate 13 is fixed by connecting the connecting bolts screwed to the two ends of the mounting plate 13 with the screw holes formed in the surface of the horizontal plate 1 or the screw holes formed in the other side of the vertical plate 2.

Furthermore, the front end and the rear end of the other side of the connecting plate 15 are both transversely and fixedly connected with connecting blocks 16, two bidirectional threaded rods 17 are rotatably arranged between the connecting blocks 16, the threads of the two ends of the surfaces of the bidirectional threaded rods 17 are opposite, the threads of the two ends of the surfaces of the bidirectional threaded rods 17 are both connected with thread blocks 18 in a threaded manner, and the clamping plate 19 is fixedly connected with the corresponding surfaces of the thread blocks 18.

In this embodiment, the two-way threaded rod 17 can rotate clockwise or counterclockwise, so that the two thread blocks 18 are close to or far away from each other, the distance between the two clamping plates 19 is adjusted, the distance between the two clamping plates 19 is increased or decreased, the clamping installation of a specified position is completed, the surface of the thread block 18 is in sliding connection with the other side of the connecting plate 15, and the movement effect of the thread block 18 is ensured.

The utility model discloses a theory of operation and use flow: the utility model discloses install the back, through place chromatography column 8 in protection box 5 inner chamber and be located between pan feeding pipe 6 and the conveyer pipe 7, rotate through dwang 21 and drive limiting plate 22 and rotate to the horizontality, thereby block chromatography column 8, make things convenient for follow-up chromatography column 8 to be connected with pan feeding pipe 6 and conveyer pipe 7, carry the sample to chromatography column 8 through pan feeding pipe 6, carry out the stratification analysis of protein through chromatography column 8, after the purification, carry the sample to protein analysis equipment 9 through conveyer pipe 7, detect through protein analysis equipment 9, when making conveyer pipe 7 carry liquid to different bin 10 by branch material pipe 11 according to the testing result, make qualified and unqualified sample separately deposit, make things convenient for follow-up unified ejection of compact, when needs are installed according to the user demand, through installing mounting panel 13 to the opposite side of riser 2 or the bottom of diaphragm 1, after the installation is accomplished, rotate drive connecting plate 15 through axis of rotation 14 and rotate, after rotating to the suitable position, can make two grip blocks 18 drive two grip blocks through forward rotation two threaded rod blocks 18 each other, thereby two reverse threaded block of installation of fixing device are close to the completion, thereby two installation of screw blocks need cancel when two cancellation installation of cancellation, 18 cancel the installation of the installation, two reverse threaded block 19, thereby two installation of cancellation.

The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.

Claims

1. A protein purification chromatography equipment which characterized in that: comprises a transverse plate (1), an installation mechanism and a clamping mechanism;

the other end of the top of the transverse plate (1) is vertically and fixedly connected with a vertical plate (2), the other end of the top of the transverse plate (1) is vertically and fixedly connected with a fixed box (3), one side of the fixed box (3) is vertically and fixedly connected with an installation box (4), an inner cavity of the installation box (4) is vertically and fixedly connected with protein analysis equipment (9), one side of the installation box (4) is vertically and fixedly connected with a protection box (5), the top of the protection box (5) is vertically communicated with a feeding pipe (6), the bottom of the inner cavity of the protection box (5) is communicated with a conveying pipe (7), the bottom end of the conveying pipe (7) penetrates from the bottom of the installation box (4) to the top of the inner cavity of the fixed box (3) through the protein analysis equipment (9), the surface of the conveying pipe (7) is vertically communicated with the inner cavity of the fixed box (3) and provided with a plurality of material distribution pipes (11), and a chromatography column (8) is detachably arranged between the bottom of the protection box (5) and the top of the feeding pipe (6) and in the inner cavity of the protection box (5);

the mounting mechanism comprises two fixed columns (20), one ends of the fixed columns (20) are rotatably provided with limiting plates (22), and the inner side surfaces of the limiting plates (22) are in contact with the surfaces of the chromatographic columns (8);

fixture detachably set up in the opposite side of riser (2), fixture includes mounting panel (13), the opposite side of mounting panel (13) rotationally transversely is provided with axis of rotation (14), the other end fixedly connected with connecting plate (15) of axis of rotation (14), the front end and the equal sliding connection in rear end of connecting plate (15) opposite side have grip block (19).

2. The protein purification chromatography apparatus of claim 1, wherein: one side of the protective box (5) is vertically provided with a through hole, and one side of the protective box (5) is detachably and vertically provided with a disassembling plate capable of plugging the through hole.

3. The protein purification chromatography apparatus of claim 1, wherein: the equal vertical connection in both sides of fixed case (3) inner chamber has bin (10), and is a plurality of divide material pipe (11) to run through respectively to corresponding bin (10) inner chamber, the vertical intercommunication in bottom of bin (10) inner chamber is provided with discharging pipe (12), the bottom of discharging pipe (12) run through extremely the bottom of fixed case (3).

4. The protein purification chromatography apparatus of claim 1, wherein: the top of mounting panel (13) transversely runs through with the equal threaded connection ground in bottom and is provided with connecting bolt, the top and the bottom of riser (2) opposite side with the both ends of diaphragm (1) bottom all seted up with the screw hole of connecting bolt looks adaptation.

5. The protein purification chromatography apparatus of claim 1, wherein: the connector has been seted up to the one end of fixed column (20) inwards cavedly, the connector inner chamber rotationally transversely is provided with dwang (21), the one end of dwang (21) extend to the outside of connector and with limiting plate (22) fixed connection.

6. The protein purification chromatography apparatus of claim 1, wherein: the front end of connecting plate (15) opposite side and the equal horizontal fixedly connected with connecting block (16) in rear end, two rotationally be provided with two-way threaded rod (17) between connecting block (16), the both ends screw thread on two-way threaded rod (17) surface is opposite, the equal threaded connection in both ends on two-way threaded rod (17) surface has screw block (18), grip block (19) and corresponding screw block (18) fixed surface is connected.