CN218454184U - Cell inoculation device - Google Patents

Abstract

A cell inoculation device comprises a liquid storage chamber, a liquid inlet channel, a liquid suction channel, a pressurization channel, a liquid outlet channel, a preparation chamber and other structures which are built in the cell inoculation device by adopting a micro-nano processing technology, wherein the cell inoculation device comprises a base, a support, and an inoculation micro-needle, a mechanical arm, a liquid storage tank, a negative pressure micro-pump, an inoculation table, a conveyor belt system and the like which are fixed on the base. The beneficial effects of the utility model are that, can combine fluorescence display to the even spraying of certain array on the inoculation flat plate of culture medium to the cell, conveniently fix a position, analyze, select the cell community to degree of automation is high, the flux is high, has improved the efficiency of cell screening.

Description

Cell inoculation device

Technical Field

The utility model relates to a biological medicine equipment, more specifically relates to a cell inoculation device.

Background

The biological pharmacy is a medical industry field which is developed and developed vigorously in the twenty-first century, main products comprise recombinant protein drugs, monoclonal antibody drugs and the like, the biological pharmacy has excellent curative effect in the treatment of various major diseases such as malignant tumors, autoimmune diseases, genetic diseases, infectious diseases and the like, the biological pharmacy has been developed into industries with annual output value reaching the billion dollar level at present, the global research and development enthusiasm is promoted, and more research institutions and pharmaceutical enterprises are put into the research, development and production of biological drugs.

Prokaryotic cells have the advantages of simple culture conditions, rapid proliferation, high expression quantity and low cost, and are the main expression host cells of biopharmaceuticals. However, with the increasing medical demand for recombinant proteins with large molecular weight, complex structure, and complex post-translational modifications of monoclonal antibodies, and the development of cell culture technology, mammalian cell expression systems have become more widely used in biopharmaceuticals. Among monoclonal antibodies approved as drugs on the market, 90% or more of them are expressed by mammalian cells such as chinese hamster ovary cells and mouse hybridoma cells.

In order to obtain engineering cell strains with high expression quantity, the construction of a mammalian cell expression system needs a great deal of cell screening and monoclonality work, and the cell screening is greatly facilitated by the appearance of a semisolid culture medium combined with a fluorescence screening technology. However, the current inoculation of the semisolid culture medium adopts a plate coating mode, the distribution of cell colonies is messy and irregular, the selection is not facilitated, and the monoclonality work is carried out after the cell selection.

Disclosure of Invention

In order to improve the convenience of screening flux, degree of automation and screening of the monoclonal cells, the utility model provides a cell inoculation device can realize the inoculation of the monoclonal high-flux matrix of cell.

The technical scheme of the utility model as follows:

a cell inoculation micro needle (20) comprises a liquid storage chamber (1), a liquid inlet channel (2), a liquid suction channel (3), a pressurizing channel (4), a liquid outlet channel (5) and a preparation chamber (13) which are constructed in the micro-nano processing technology; the liquid inlet channel (2), the liquid suction channel (3), the pressurizing channel (4) and the liquid outlet channel (5) are intersected in the preparation chamber (13); the diameter of the liquid suction channel (3) is smaller than that of the liquid inlet channel (2), preferably, the diameter of the liquid inlet channel is between 15 and 25 micrometers, and the diameter of the liquid suction channel is between 5 and 10 micrometers; a push rod (6) is embedded in the pressurizing channel (4), the push rod (6) is connected with a piezoelectric ceramic module (7), and the piezoelectric ceramic module (7) is connected with an electrifying interface (9) on the outer surface through a lead (8); the liquid inlet passage (2) and the liquid suction passage (3) are respectively provided with a liquid inlet interface (10) and a liquid suction interface (11) on the outer surface; the outer surface is also provided with a fixing bayonet (12) for fixing the inoculation micro-needle (20).

A cell inoculation device comprises a base (21), a support (22), a mechanical arm (23), a liquid storage box (25), a negative pressure micro pump (26), an inoculation table (27), a conveyor belt system (28), a stepping motor (31) and an integrated circuit controller (50), wherein the mechanical arm (23), the liquid storage box (25), the negative pressure micro pump, the inoculation table (27) and the integrated circuit controller are fixed on the base (21) or the support (22). An inoculation needle frame (24) is fixed on the mechanical arm (23), 1-256 inoculation micro needles (20) are fixed on the inoculation needle frame (24), the inoculation micro needles (20) are arranged on the inoculation needle frame (24) in a straight line or a matrix, and the fixed bayonet (12) on the inoculation micro needles (20) is embedded with the clamping seat (30) on the inoculation needle frame (24) to realize fixation. The liquid storage tank (25) is higher than the inoculation micro-needle (20) and is hermetically connected with the liquid inlet interface (10) through a soft catheter; the negative pressure micro pump (26) is hermetically connected with the liquid suction interface (11) of the inoculation micro needle (20) through a pipeline; the inoculation plate (29) loaded with cell culture medium is placed on a conveyor system (28) and transported to an inoculation station (27). The mechanical arm (23) is connected with and controlled by a stepping motor (31). The power-on interface (9) of the inoculation micro-needle (20), the negative pressure micro-pump (26), the conveyor belt system (28) and the stepping motor (31) are all connected with and controlled by the integrated circuit controller (50). The controller (50) may be connected to a computer and programmed.

The utility model discloses a use method and the operating mechanism of inoculation micropin (20) are: the solution containing the cells is added to the reservoir (25) and the controller (50) initiates the "seeding procedure": opening a negative pressure pump (26) to apply negative pressure to the inner cavity of the inoculation micro needle (20) through the liquid suction channel (3), sucking the cell solution into the liquid storage chamber (1), then applying micro negative pressure, pumping the cell solution from the liquid storage chamber into the liquid inlet channel (3), and staying the cells in the preparation chamber (13); the controller (50) gives an electric signal to the piezoelectric ceramic module (7) through the power-on interface (9) and the lead (8), the piezoelectric ceramic deforms, the push rod (6) is pushed to move, and cells in the preparation chamber (13) are ejected to the surface of the inoculation flat plate (29) through the liquid outlet channel (5).

The utility model discloses a cell inoculation device's application method and operating mechanism are: the inoculation micro-needles (20) are arranged on an inoculation needle frame (24) according to the required quantity and arrangement mode, the components of the cell inoculation device are connected in the connection mode, and the controller (50) starts a transmission program: driving the conveyor system (28) to transport the inoculation plate (29) beneath the inoculation microneedles (20); the controller (50) then initiates a positioning procedure: a stepping motor (31) drives a mechanical arm (23) to precisely position an inoculation microneedle (20) at an inoculation position of an inoculation plate (29); finally, the controller (50) initiates an inoculation procedure to eject the cells onto the surface of the inoculation plate (29).

The beneficial effects of the utility model are that, can combine fluorescence display to the even spraying of certain array on the inoculation flat plate of culture medium to the cell, conveniently fix a position, analyze, select the cell community to degree of automation is high, the flux is high, has improved the efficiency of cell screening.

Drawings

FIG. 1A structural diagram of a cell-seeded microneedle

FIG. 2A view showing the structure of a cell seeding apparatus

Reference numerals: 1-liquid storage chamber, 2-liquid inlet channel, 3-liquid suction channel, 4-pressurizing channel, 5-liquid outlet channel, 6-push rod, 7-piezoelectric module, 8-lead, 9-electrifying interface, 10-liquid inlet interface, 11-liquid suction interface, 12-fixing bayonet, 13-preparation chamber, 20-inoculation micro needle, 21-base, 22-bracket, 23-mechanical arm, 24-inoculation needle bracket, 25-liquid storage tank, 26-negative pressure micro pump, 27-inoculation table, 28-conveyor belt system, 29-inoculation flat plate, 30-clamping seat, 31-stepping motor and 50-controller.

Detailed Description

Example 1 Structure of cell-seeded microneedle

A cell inoculation device is designed into an inoculation microneedle (20), and comprises a liquid storage chamber (1), a liquid inlet channel (2), a liquid suction channel (3), a pressurization channel (4), a liquid outlet channel (5) and a preparation chamber (13) which are constructed in the micro-nano processing technology; the liquid inlet channel (2), the liquid suction channel (3), the pressurizing channel (4) and the liquid outlet channel (5) are intersected in the preparation chamber (13); the diameter of the liquid suction passage (3) is smaller than that of the liquid inlet passage (2), the diameter of the liquid inlet passage is 20 micrometers, and the diameter of the liquid suction passage is 8 micrometers; a push rod (6) is embedded in the pressurizing channel (4), the push rod (6) is connected with a piezoelectric ceramic module (7), and the piezoelectric ceramic module (7) is connected with an electrifying interface (9) on the outer surface through a lead (8); the liquid inlet passage (2) and the liquid suction passage (3) are respectively provided with a liquid inlet interface (10) and a liquid suction interface (11) on the outer surface; the outer surface is also provided with a fixing bayonet (12) for fixing the inoculation micro-needle (20).

EXAMPLE 2 Structure of cell seeding apparatus

A cell inoculation device comprises a base (21), a support (22), a mechanical arm (23) fixed on the base (21) or the support (22), a liquid storage box (25), a negative pressure micro pump (26), an inoculation platform (27), a conveyor belt system (28), a stepping motor (31) and an integrated circuit controller (50). An inoculation needle frame (24) is fixed on the mechanical arm (23), 12 inoculation micro needles (20) are fixed on the inoculation needle frame (24), the inoculation micro needles (20) are linearly arranged on the inoculation needle frame (24), and the fixing bayonets (12) on the inoculation micro needles (20) are fixed with the bayonets (30) on the inoculation needle frame (24) through embedding. The liquid storage tank (25) is higher than the inoculation micro-needle (20) and is hermetically connected with the liquid inlet interface (10) through a soft catheter; the negative pressure micro pump (26) is hermetically connected with the liquid suction interface (11) of the inoculation micro needle (20) through a pipeline; the inoculation plate (29) loaded with cell culture medium is placed on a conveyor system (28) and transported to an inoculation station (27). The mechanical arm (23) is connected with and controlled by a stepping motor (31). The power-on interface (9) of the inoculation micro-needle (20), the negative pressure micro-pump (26), the conveyor belt system (28) and the stepping motor (31) are all connected with and controlled by the integrated circuit controller (50). The controller (50) is connected to the computer and is programmed.

Example 3 cell inoculation microneedle, and method and mechanism for using cell inoculation device

The utility model discloses a use method and the operating mechanism of inoculation micropin (20) are: the solution containing the cells is added to the reservoir (25), and the controller (50) initiates a "seeding procedure": opening a negative pressure pump (26) to apply negative pressure to the inner cavity of the inoculating microneedle (20) through the liquid suction channel (3) to suck the cell solution into the liquid storage chamber (1), then applying a micro negative pressure to pump the cell solution from the liquid storage chamber into the liquid inlet channel (3), and enabling the cells to stay in the preparation chamber (13); the controller (50) gives an electric signal to the piezoelectric ceramic module (7) through the electrifying interface (9) and the lead (8), the piezoelectric ceramic deforms, the push rod (6) is pushed to move, and cells in the preparation chamber (13) are ejected to the surface of the inoculation flat plate (29) through the liquid outlet channel (5).

The utility model discloses a cell inoculation device's application method and operating mechanism are: the inoculation micro-needles (20) are arranged on an inoculation needle frame (24) according to the required quantity and arrangement mode, the components of the cell inoculation device are connected in the connection mode, and the controller (50) starts a transmission program: driving the conveyor system (28) to transport the inoculation plate (29) beneath the inoculation microneedles (20); the controller (50) then initiates a positioning procedure: a stepping motor (31) drives a mechanical arm (23) to precisely position an inoculation microneedle (20) at an inoculation position of an inoculation plate (29); finally, the controller (50) initiates an inoculation procedure to eject the cells onto the surface of the inoculation plate (29).

Claims (2)

1. A cell inoculation device is characterized in that the cell inoculation device is an inoculation micro needle (20) and comprises a liquid storage chamber (1), a liquid inlet channel (2), a liquid absorption channel (3), a pressurization channel (4), a liquid outlet channel (5) and a preparation chamber (13) which are constructed in the cell inoculation device by adopting a micro-nano processing technology; the liquid inlet channel (2), the liquid suction channel (3), the pressurizing channel (4) and the liquid outlet channel (5) are intersected in the preparation chamber (13); the diameter of the liquid inlet channel (2) is between 15 and 25 micrometers, and the diameter of the liquid absorbing channel (3) is between 5 and 10 micrometers; a push rod (6) is embedded in the pressurizing channel (4), the push rod (6) is connected with a piezoelectric ceramic module (7), and the piezoelectric ceramic module (7) is connected with an electrifying interface (9) on the shell through a lead (8); the liquid inlet passage (2) and the liquid suction passage (3) are respectively provided with a liquid inlet interface (10) and a liquid suction interface (11) on the outer surface; the outer surface is provided with a fixing bayonet (12) for fixing the inoculation micro-needle (20).

2. A cell inoculation device is characterized by comprising a base (21), a support (22), a mechanical arm (23), a liquid storage tank (25), a negative pressure micropump (26), an inoculation table (27), a conveyor belt system (28), a stepping motor (31) and an integrated circuit controller (50), wherein the mechanical arm (23), the liquid storage tank (25), the negative pressure micropump (26), the inoculation table (27), the conveyor belt system (28) and the integrated circuit controller (50) are fixed on the base (21) or the support (22); an inoculation needle frame (24) is fixed on the mechanical arm (23), 1-256 inoculation micro needles (20) according to claim 1 are fixed on the inoculation needle frame (24), the inoculation micro needles (20) are arranged on the inoculation needle frame (24) in a straight line or a matrix, and a fixing bayonet (12) on the inoculation micro needles (20) is fixed with a clamping seat (30) on the inoculation needle frame (24) through embedding; the liquid storage tank (25) is hermetically connected with the liquid inlet interface (10) through a soft guide pipe; the negative pressure micro pump (26) is hermetically connected with the liquid suction interface (11) of the inoculation micro needle (20) through a pipeline; the mechanical arm (23) is connected with a stepping motor (31); the power-on interface (9) of the inoculation micro needle (20), the negative pressure micro pump (26), the conveyor belt system (28) and the stepping motor (31) are all connected with the integrated circuit controller (50).

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