CN218435781U

CN218435781U - Micro-tumor cell culture device for colorectal cancer

Info

Publication number: CN218435781U
Application number: CN202222659947.6U
Authority: CN
Inventors: 徐敏
Original assignee: Zhengzhou University
Current assignee: Zhengzhou University
Priority date: 2022-10-10
Filing date: 2022-10-10
Publication date: 2023-02-03
Anticipated expiration: 2032-10-10

Abstract

The utility model relates to the field of biotechnology, especially, relate to a micro-tumor cell culture device of colorectal cancer. The colorectal cancer micro-tumor cell culture device can facilitate people to pour culture solution when the culture solution is poured and purified for cancer cells, and reduces the manual operation difficulty and pollution. The utility model provides a little tumour cell culture apparatus of colorectal cancer, includes box, chamber door and places the seat etc. and two front sides rotation type are connected with the chamber door about the box, and the rotary type is connected with a plurality of seats of placing in the box. The utility model discloses a first motor of both sides drives the lead screw and rotates for the guide block carries out the back-and-forth movement under the effect of lead screw, thereby makes the rotating turret drive and is emptyd work by the tight culture dish of clamping piece clamp after, and then has reached and has made things convenient for people to emptys the culture solution, prevents that manual operation from causing the culture solution to topple over and spill unevenly, influences the effect that follow-up work goes on.

Description

Micro-tumor cell culture device for colorectal cancer

Technical Field

The utility model relates to the field of biotechnology, especially, relate to a micro-tumor cell culture device of colorectal cancer.

Background

Colorectal cancer is one of numerous malignant tumors which seriously threaten human life and affect human health, the recurrence and metastasis rate of the colorectal cancer is high and difficult to cure radically, patients can have recurrence and metastasis of different degrees within months to years after radical treatment, and the colorectal cancer cell can be cultured in order to know the biological processes of occurrence, development, recurrence and metastasis and the like of the disease, so that research and observation are carried out, and the colorectal cancer can be overcome early.

Present rectum cancer tumor cell culture apparatus, place in the culture solution through the cancer cell sample, breed the maintenance, purify the cancer cell after reaching a certain amount until the cancer cell, derive the culture solution in the culture dish during the purification, the cancer cell can flow out along the culture solution, and miscellaneous cell in the culture dish can be because cell characteristic adsorbs at the culture dish inner wall, through empting the culture solution repeatedly, reach the purpose of purification cancer cell, but manual operation pours to the culture dish, the problem that hand trembles and hand pollution appears easily, the cancer cell that leads to carefully looking after appears infecting and the culture solution pours and leads to the fact inconvenient problem.

To the above-mentioned problem, developed now one kind and when purifying and pouring the culture solution to cancer cell, can make things convenient for people to pour the culture solution, reduced the little tumour cell culture apparatus of colorectal cancer of the manual operation degree of difficulty and pollution.

SUMMERY OF THE UTILITY MODEL

When empting the cancer cell purification in order to overcome manual operation to the culture dish, the problem that the hand trembles and the hand pollutes appears easily, leads to the cancer cell of carefully taking care to appear infecting and the culture solution is toppled over and is caused inconvenient shortcoming, provides one kind and when purifying to cancer cell and toppling over the culture solution, can make things convenient for people to topple over the culture solution, reduces the colorectal cancer micro tumor cell culture apparatus of the manual operation degree of difficulty and pollution.

The technical scheme is as follows: the utility model provides a little tumour cell culture apparatus of carcinoma of colon, including box, chamber door, place seat, culture dish, bed hedgehopping platform and empty the mechanism, two front sides rotation type is connected with the chamber door about the box, and the box rotary type is connected with a plurality of seats of placing, places and all has placed the culture dish on the seat, and the inside intermediate junction of box has the bed hedgehopping platform, and the culture dish has also been placed at bed hedgehopping bench top, and the bed hedgehopping bench is equipped with empting the mechanism.

Further, topple over the mechanism including the installed part, the lead screw, the guide block, spacing post, first motor, the rotating turret, clamping piece and spring, two upsides all are connected with the installed part about the bed hedgehopping platform, the equal rotary type in installed part lower part is connected with the lead screw, two upsides all are connected with first motor about the bed hedgehopping platform, the first motor output shaft of the left and right sides is connected with the lead screw of the left and right sides respectively, the installed part lower part all is connected with spacing post, all be connected with the guide block between spacing post and the lead screw, guide block and lead screw threaded connection, guide block and spacing post slidingtype are connected, the rotary type is connected with the rotating turret between the installed part upper portion, the rotating turret front portion is connected with the guide block rotary type of the left and right sides respectively, the equal slidingtype in rotating turret rear portion is connected with the clamping piece, all be connected with the spring between clamping piece and the rotating turret.

Further, still including slewing mechanism, the slewing mechanism board all is connected with the second motor including ring gear, gear and second motor, the inside left and right sides of box, all is connected with the gear on the second motor output shaft, all is connected with the ring gear on placing the seat, ring gear and gear engagement.

Furthermore, the box doors are all provided with observation windows.

Furthermore, the liquid guide part of the culture dish is in a sharp mouth shape.

Further, the clamping piece is provided with a pulling handle.

The utility model has the advantages of it is following: 1. the utility model discloses a first motor of both sides drives the lead screw and rotates for the guide block carries out the back-and-forth movement under the effect of lead screw, thereby makes the rotating turret drive and is emptyd work by the tight culture dish of clamping piece clamp after, and then has reached and has made things convenient for people to emptys the culture solution, prevents that manual operation from causing the culture solution to topple over and spill unevenly, influences the effect that follow-up work goes on.

2. The utility model discloses a second motor output shaft drives the gear and rotates, and rethread gear drives the ring gear and rotates for place the purpose that the seat drove the culture dish and rotate and realize stirring culture solution, thereby make when stirring the culture solution, can reach and reduce the manual operation and cause the culture solution to pollute, and can stir the culture solution fast, improve the effect of cultivateing efficiency.

Drawings

Fig. 1 is a schematic perspective view of the present invention.

Fig. 2 is a schematic structural diagram of the present invention.

Fig. 3 is a schematic view of a partial three-dimensional structure of the present invention.

Fig. 4 is a schematic view of the three-dimensional structure of the dumping mechanism of the present invention.

Fig. 5 is a schematic view of a partial three-dimensional structure of the dumping mechanism of the present invention.

Fig. 6 is a schematic view of the three-dimensional structure of the rotating mechanism of the present invention.

Wherein: 1-box body, 2-box door, 3-placing seat, 4-culture dish, 5-heightening table, 6-mounting piece, 7-screw rod, 8-guide block, 9-limiting column, 10-first motor, 11-rotating frame, 12-clamping piece, 13-spring, 14-gear ring, 15-gear and 16-second motor.

Detailed Description

The invention will be further described with reference to specific examples, which are intended to illustrate, unless otherwise explicitly specified or limited, terms such as: the arrangement, installation, connection are to be understood broadly, for example, they may be fixed, detachable, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The utility model provides a little tumour cell culture apparatus of colorectal cancer, as shown in fig. 1-4, including box 1, chamber door 2, place seat 3, culture dish 4, fill up high platform 5 and empty the mechanism, box 1 is controlled two front sides rotation type and is connected with chamber door 2, all be equipped with the observation window on the chamber door 2, make things convenient for people to observe 1 internal state of box, inside culture dish 4 can be protected to chamber door 2, prevent that external bacterium from causing the infection to the cancer cell in the

culture dish

4, 1 internal rotation type of box is connected with a plurality of seats 3 of placing, place 3 total six of seat, every three is a set of, each a set of seat 3 of placing of two portions about in the box 1, culture dish 4 has all been placed on placing seat 3, culture dish 4 drain position is the point mouth form, the convenience is derived cancer cell culture solution when empting, 1 internal intermediate junction has fill up high platform 5, culture dish 4 has also been placed at fill up high platform 5 top, be equipped with the mechanism of empting that is used for empting culture dish 4 on fill up high platform 5.

As shown in fig. 4 and 5, the dumping mechanism comprises a mounting member 6, a screw rod 7, a guide block 8, a limit post 9, a first motor 10, a rotating frame 11, a clamping member 12 and a spring 13, wherein the mounting member 6 is connected to the upper sides of the left and right parts of the heightening table 5, the screw rod 7 is connected to the lower part of the mounting member 6 in a rotating manner, the first motor 10 is connected to the upper sides of the left and right parts of the heightening table 5, the output shafts of the first motors 10 on the left and right sides are respectively connected to the screw rods 7 on the left and right sides, the limit post 9 is connected to the lower part of the mounting member 6, the guide block 8 is connected between the limit post 9 and the screw rod 7, the guide block 8 is in threaded connection with the screw rod 7, the guide block 8 is in sliding connection with the limit post 9, the rotating frame 11 is connected between the upper parts of the mounting member 6 in a rotating manner, the front part of the rotating frame 11 is respectively connected with the guide block 8 on the left and right sides in a rotating manner, the rear part of the rotating frame 11 is connected with the clamping member 12 in a sliding manner, from having the pulling handle on the clamping piece 12, can make things convenient for people to pull clamping piece 12, place and press from both sides tight culture dish 4, all be connected with spring 13 between clamping piece 12 and the rotating turret 11, pulling clamping piece 12, make clamping piece 12 keep away from each other, clamping piece 12 is when keeping away from the motion each other, can extrude spring 13, spring 13 atress warp, later place culture dish 4 on rotating turret 11, loosen clamping piece 12 again, clamping piece 12 resets under spring 13's effect, press from both sides tight fixed culture dish 4, later start the first motor 10 of the left and right sides, first motor 10 output shaft drives lead screw 7 and rotates, make guide block 8 remove backward, thereby make rotating turret 11 overturn and drive culture dish 4 and overturn, it arrives the effect of empting the culture solution in the culture dish 4 to transfer.

In order to understand the biological processes of colorectal cancer, such as occurrence, development, recurrence and metastasis, colorectal cancer cells are cultured, so as to study and observe, the colorectal cancer cells can be favorably combated early, the device can be used when the colorectal cancer cells are cultured, firstly, culture solution is led into a culture dish 4, then, the colorectal cancer cells needing to be cultured are led into the culture dish 4 for culture, after the colorectal cancer cells are cultured to a certain number, the cancer cells in the culture dish 4 need to be purified, some miscellaneous cells are removed, therefore, the culture dish 4 needing to be purified can be placed on a rotating frame 11, when the culture dish is placed, two clamping pieces 12 are pulled firstly, the clamping pieces 12 on the two sides are forced to be away from each other to press springs 13 on the two sides, the springs 13 are compressed to enable the clamping pieces 12 to improve the placing space for the culture dish 4, after the culture dish 4 is placed, the clamping piece 12 is loosened to restore the spring 13, the clamping piece 12 is pushed to clamp and fix the culture dish 4, then the first motors 10 on the two sides are started, the output shaft of the first motor 10 drives the screw rod 7 to rotate, the guide blocks 8 on the two sides move backwards along the limiting columns 9 under the action of the screw rod 7, when the guide blocks 8 move, the rotating frame 11 can be rotated gradually, the clamped culture dish 4 can also be rotated gradually, when the rotation is performed, because the rotating point of the rotating frame 11 and the liquid outlet position of the culture dish 4 are in the same horizontal line, the toppling position of the culture dish 4 can be invariable all the time, the problem that the subsequent operation is influenced by uneven toppling of the culture solution and the colorectal cancer cells due to shaking can be avoided, when the toppling is completed, because the characteristics of the mixed cells and the cancer cells, the mixed cells in the culture solution can be adsorbed on the inner wall of the culture dish 4, and the colorectal cancer cell then can stay in the culture solution, flow along with the culture solution and derive, the culture solution of derivation and colorectal cancer cell can fall into in the new culture dish 4 on the elevating platform 5, carry out the purification of colorectal cancer cell, operating personnel can empty the culture solution in the culture dish 4 repeatedly through controlling first motor 10, further purify the colorectal cancer cell in the culture dish 4, empty after the purification is accomplished, first motor 10 output shaft drives lead screw 7 and rotates, make guide block 8 remove forward gradually and reset, make rotating turret 11 rotate gradually under the effect of guide block 8 and reset, make the culture dish 4 of being pressed from both sides gradually be the level and place, later pulling clamping piece 12 takes out culture dish 4, clean, spring 13 recovers when clamping piece 12 loosens makes clamping piece 12 reset, make things convenient for operating personnel to use next time, after rotating turret 11 and guide block 8 reset and accomplish, close the first motor 10 of both sides, to sum up, first motor 10 through both sides drives lead screw 7 and rotates, make guide block 8 carry out the effect of leading, thereby make the effect of the guide block 8 carry out and carry out the maintenance, thereby make the rotating turret 11 drive the culture dish that inclines and empty the manual work liquid that has all caused to pour out, thereby the influence of the culture dish that people to pour out, the convenient pouring, the culture dish is poured out and has reached after the effect of pouring.

As shown in fig. 6, the cell culture plate further comprises a rotating mechanism, the rotating mechanism plate comprises a gear ring 14, a gear 15 and a second motor 16, the second motor 16 is connected to the left side and the right side inside the box body 1, the gear 15 is connected to the output shaft of the second motor 16, the gear ring 14 is connected to the placing seat 3, the gear ring 14 is meshed with the gear 15, the gear 15 is driven by the output shaft of the second motor 16 to rotate, the gear 15 drives the placing seat 3 to rotate through the gear ring 14, and then the placing seat 3 drives the culture dish 4 to rotate, so that the purpose of stirring the culture solution to promote the mutual fusion of cells is achieved.

When carrying out cancer cell culture, generally need stir the culture solution, promote the colorectal cancer cell in the culture solution to propagate and grow, consequently, can start the second motor 16 of both sides, second motor 16 output shaft drives gear 15 and rotates, it is rotatory again to drive ring gear 14 by gear 15, make place seat 3 rotate, place seat 3 when taking place to rotate, can drive culture dish 4 on placing seat 3 and rotate, culture dish 4 rotates and to make inside culture solution appear rocking, thereby reach the purpose of stirring, after the rotatory stirring of culture solution in culture dish 4 is accomplished, close second motor 16 of both sides, make place seat 3 and culture dish 4 stop the stirring, operating personnel can control second motor 16 according to the on-the-spot needs, realize the quick stirring work, be convenient for operating personnel operate, to sum up, drive gear 15 through second motor 16 output shaft and rotate, rethread gear 15 drives ring gear 14 and rotates, make place seat 3 and drive culture dish 4 and rotate the purpose of realizing the stirring culture solution, thereby make when stirring the culture solution, can reduce the artificial operation solution and the culture solution that reduces, the culture solution and can cause the pollution to the fast efficiency, the culture solution, the effect of stirring.

The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.

Claims

1. The utility model provides a little tumour cell culture apparatus of colorectal cancer, a serial communication port, including box (1), chamber door (2), place seat (3), culture dish (4), bed hedgehopping platform (5) and empty the mechanism, box (1) is controlled two front sides and is rotated and be connected with chamber door (2), box (1) internal rotation formula is connected with a plurality of seats (3) of placing, culture dish (4) have all been placed on placing seat (3), box (1) inside intermediate junction has bed hedgehopping platform (5), culture dish (4) have also been placed at bed hedgehopping platform (5) top, be equipped with on bed hedgehopping platform (5) and empty the mechanism.

2. The colorectal cancer micro-tumor cell culture device according to claim 1, wherein the dumping mechanism comprises a mounting part (6), a lead screw (7), a guide block (8), a limiting column (9), a first motor (10), a rotating frame (11), a clamping part (12) and a spring (13), the mounting part (6) is connected to the upper sides of the left part and the right part of the elevating table (5), the lead screw (7) is rotatably connected to the lower part of the mounting part (6), the first motor (10) is connected to the upper sides of the left part and the right part of the elevating table (5), the output shaft of the first motor (10) on the left side and the right side is rotatably connected to the lead screw (7) on the left side and the right side respectively, the limiting column (9) is connected to the lower part of the mounting part (6), the guide block (8) is connected to the front part of the rotating frame (11), the guide block (8) is threadedly connected to the lead screw (7), the guide block (8) is slidably connected to the limiting column (9), the rotating frame (11) is rotatably connected to the rear part of the rotating frame (12), and the clamping part (12) is rotatably connected to the rotating frame (11).

3. The colorectal cancer micro-tumor cell culture device according to claim 2, further comprising a rotating mechanism, wherein the rotating mechanism plate comprises a gear ring (14), a gear (15) and a second motor (16), the second motor (16) is connected to the left side and the right side inside the box body (1), the gear (15) is connected to the output shaft of the second motor (16), the gear ring (14) is connected to the placing seat (3), and the gear ring (14) is meshed with the gear (15).

4. The device for culturing micro-tumor cells of colorectal cancer according to claim 1, wherein the box doors (2) are provided with observation windows.

5. The apparatus as claimed in claim 1, wherein the drainage portion of the dish (4) is in the shape of a sharp nozzle.

6. The device for culturing the micro-tumor cells of colorectal cancer according to claim 2, wherein the clamping member (12) is provided with a pulling handle.