CN218411922U - Blood sampling test tube, support and test tube assembly - Google Patents
Blood sampling test tube, support and test tube assembly Download PDFInfo
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- CN218411922U CN218411922U CN202222117733.6U CN202222117733U CN218411922U CN 218411922 U CN218411922 U CN 218411922U CN 202222117733 U CN202222117733 U CN 202222117733U CN 218411922 U CN218411922 U CN 218411922U
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Abstract
The utility model discloses a blood sampling test tube, support and test tube subassembly, the blood sampling test tube include the test tube, sealed lid in the sealing plug of test tube port, the inside vacuum of test tube, it has the anticoagulant to embed in the test tube, the immune magnetic bead freeze-drying microballon is equipped with in the test tube. The utility model does not need to use centrifugation and filtration to separate blood plasma and blood cells; the use of the plasma separation device is simplified, and the blood sample can be directly used for an immunological detection instrument; red blood cells in a fresh whole blood sample can be rapidly separated; the utility model discloses well blood sampling test tube directly is whole blood processing apparatus.
Description
Technical Field
The utility model relates to a blood sampling test tube field, especially a blood sampling test tube, support and test tube subassembly.
Background
The traditional venous blood sampling method is that samples are collected through a blood sampling tube added with an anticoagulant, a common method for separating red blood cells from the blood samples to obtain serum or plasma is centrifugal separation (more than 5 minutes), special centrifugal equipment is required, and the requirement for rapid detection of clinical immunoassay is difficult to meet. The centrifugation method is difficult to realize the automation of red blood cell separation, is not beneficial to the miniaturization of detection equipment, and the existence of red blood cells brings great interference to the existing blood marker detection. The blood sample treatment and detection can be performed quickly and accurately, and a treatment time window can be won for a patient.
The technology for obtaining plasma by whole blood sample pretreatment is mainly researched by a hemofiltration membrane, a sample pad (with anti-RBC antibody attached to the surface) and a microsphere carrier (phytohemagglutinin or anti-RBC antibody coated by physical adsorption or chemical coupling). The blood filter membrane and the sample pad are mainly used for separating red blood cells in a whole blood sample and are mainly used in a fluorescence immunochromatographic kit for detecting the whole blood sample, however, in the methods, on one hand, the red blood cells remained on the immunoreagent strip have obvious influence on the flow of the chromatographic reaction. On the other hand, the red blood cell debris generated by hemolysis is difficult to be trapped in the blood filtering membrane or the sample pad, and can be chromatographed on the nitrocellulose membrane along with the reaction solution, thereby influencing the immunochromatography reaction or causing strong background interference. Furthermore, the existing microsphere carriers (phytohemagglutinin or anti-RBC antibody coated by physisorption) are harsh in processing whole blood samples (no addition of any lectin or other reagents) and the separation time is too long (greater than 2 min). Clinically, a portion of the patient sample is usually pooled and tested on-machine, and the whole blood sample is limited in the test time. While other microsphere carriers (anti-RBC antibody coated by chemical coupling) have improved efficiency in red blood cell separation, the treatment time is still longer (greater than 1 min).
Chinese patent publication No. CN109998561B discloses a disposable blood coagulation vacuum blood collection tube, belonging to the technical field of medical instruments. The disposable blood coagulation vacuum blood collection tube comprises a tube body, wherein a label with scales is arranged on the tube body, and a sodium citrate anticoagulant is arranged in the tube body. Chinese patent publication No. CN111662869A discloses taking blood from a blood-taking test tube, mixing with an anticoagulant, taking fresh anticoagulated whole blood, adding into a vial containing immunomagnetic beads, and placing on a magnetic rack. Need use blood sampling test tube, xiLin bottle, magnetic rack in this patent, the device is complicated, complex operation.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that, not enough to prior art, provide a blood sampling test tube, realize the effect of anti-freezing and quickly separating erythrocyte after the blood sampling simultaneously.
The utility model also provides a with the supporting support that uses of blood sampling test tube, fixed blood sampling test tube to the realization utilizes the erythrocyte in the magnetic separation blood.
The utility model also provides a test tube subassembly realizes the effect of anti-freezing and quickly separating erythrocyte after the blood sampling simultaneously, need not with the help of other magnetic separation device.
In order to solve the technical problem, the utility model discloses the technical scheme who adopts is: the utility model provides a blood sampling test tube, includes the test tube, sealed lid in the sealing plug of test tube port, the inside vacuum of test tube, it has the anticoagulant to embed in the test tube, the test tube is equipped with immunomagnetic bead freeze-drying microsphere in.
The utility model discloses a blood sampling test tube can directly take a blood sample, and the anticoagulant in the test tube is dissolved in fresh blood, and after the misce bene, arrange magnetic means in on, can the red blood cell in the quick separation blood with the help of the freeze-dried microballon of immunomagnetic bead.
In a preferred embodiment of the present invention, the anticoagulant is coated on the bottom of the inner wall of the test tube.
In a preferred embodiment of the present invention, the anticoagulant is coated on the surface of the immunomagnetic bead freeze-dried microsphere.
In a preferred embodiment of the present invention, the particle size of the immunomagnetic bead lyophilized microsphere is less than 1 μm.
In a preferred embodiment of the present invention, the particle size of the immunomagnetic bead lyophilized microsphere is less than 0.5 μm.
In a preferred embodiment of the present invention, the particle size of the immunomagnetic bead lyophilized microsphere is 0.1-0.4 μm. The immunomagnetic bead freeze-dried microsphere with the particle size has better separation efficiency.
The utility model discloses a preferred embodiment, immunomagnetic bead freeze-drying microsphere is 1 ~ 5, according to the volume of fresh blood, selects appropriate immunomagnetic bead freeze-drying microsphere quantity.
The utility model also discloses a with supporting support that uses of blood sampling test tube, the test tube passes through the support can be dismantled fixedly, be equipped with magnet in the support, magnet is located under the test tube.
The utility model discloses a preferred embodiment, square groove has been seted up to the support inboard, place in the test tube in the square groove, be convenient for fixed test tube directly place the test tube in the square groove can.
In a preferred embodiment of the present invention, the magnet is a square magnet embedded in the bracket and located directly below the square groove.
The utility model also discloses a test tube subassembly, which comprises a bracket, be equipped with on the support the blood sampling test tube.
Compared with the prior art, the utility model discloses the beneficial effect who has does: 1. the separation of plasma and blood cells is carried out by filtration without using centrifugation; 2. the use of the plasma separation device is simplified, and the blood sample can be directly used for an immunological detection instrument; 3. red blood cells in a fresh whole blood sample can be rapidly separated; 4. the utility model discloses well blood sampling test tube directly is whole blood processing apparatus.
Drawings
Fig. 1 is a schematic sectional view of a blood collection chamber tube according to embodiment 1 of the present invention.
Fig. 2 is a schematic sectional view of a blood collection chamber tube according to embodiment 2 of the present invention.
Fig. 3 is a schematic structural diagram of an immunomagnetic bead lyophilized microsphere in embodiment 2 of the present invention.
Wherein 1 is the test tube, 2 is immunomagnetic bead freeze-drying microballon, 3 is the support, 4 is magnet, 5 is the anticoagulant, 6 is sealed lid, 7 is square groove.
Detailed Description
Example 1
As shown in fig. 1, the blood sampling test tube comprises a test tube 1, a sealing plug 6 sealing a port of the test tube 1, and a vacuum inside the test tube 1, wherein 1 immunomagnetic bead lyophilized microsphere 2 is filled in the test tube 1. The anticoagulant 5 is coated on the bottom of the inner wall of the test tube 1. The particle size of the immunomagnetic bead freeze-dried microsphere 2 is 0.4 μm.
The test tube 1 that the inner wall bottom coating has anticoagulant 5 can directly adopt current coating to have anticoagulant vacuum blood sampling test tube, arranges immunomagnetic bead freeze-drying microballon 2 in test tube 1, carries out evacuation again and handles. The immunomagnetic beads freeze-dried microspheres 2 are prepared from the immunomagnetic beads disclosed in Chinese patent with publication No. CN 111662869A.
The bracket 3 is matched with the blood sampling test tube for rapidly separating the red blood cells, the test tube 1 is fixed by the bracket 3, and a magnet 4 is arranged in the bracket 3. Square groove 7 has been seted up to support 3 inboard, place in test tube 1 in the square groove 7. Magnet 4 is square magnet, embedded in support 3, and be located under square groove 7. The support 3 is made of transparent materials by self-manufacturing, has the function of integral fixing and supporting, and is used for fixing the test tube 1. Magnet 4 is purchased for the outside, and the main function is magnetic attraction separation, guarantees that red blood cell can quick separation.
Example 2
As shown in fig. 2, this embodiment is different from embodiment 1 in that EDTA-K2 anticoagulant 5 is directly coated on the surface of immunomagnetic bead lyophilized microsphere 2, as shown in fig. 3, it is not coated on the bottom of test tube 1, anticoagulant 5 is coated on the surface of immunomagnetic bead lyophilized microsphere 2, and anticoagulant 5 is the existing anticoagulant EDTA-K2.
Collected fresh blood (without anticoagulant) in an amount of 200uL was directly added to the blood collection tube obtained in example 1 or the blood collection tube obtained in example 2, which was then capped and sealed, and shaken by hand for 20 seconds, to thereby rapidly dissolve and disperse microspheres 1 at the bottom of the tube. Then, insert the test tube and inhale the support magnetically, the separation is inhaled to magnetism, and obvious layering phenomenon can be seen: the upper layer is clear plasma and the lower layer is aggregated red blood cells.
The method for separating the red blood cells provided by the patent does not need to use a blood collection tube and directly acts on fresh blood. Meanwhile, the separation time is far shorter than the time required by natural sedimentation of red blood cells in conventional whole blood and even shorter than the separation time of the microspheres reported currently. In addition, in the using process, extra tools and operation are not needed, compared with the conventional electric centrifugal separation, the method is simpler and more reliable, and is suitable for the bedside diagnosis requirement of immunological detection.
Claims (10)
1. The utility model provides a blood sampling test tube, includes test tube (1), sealed lid in sealing plug (6) of test tube (1) port, the inside vacuum of test tube (1), it has anticoagulant (5) to embed in test tube (1), its characterized in that, immune magnetic bead freeze-drying microballon (2) are equipped with in test tube (1).
2. Blood collection tube according to claim 1, characterized in that the anticoagulant (5) is coated on the bottom of the inner wall of the tube (1).
3. The blood collection tube of claim 1, wherein the anticoagulant (5) is coated on the surface of the immunomagnetic bead lyophilized microspheres (2).
4. The blood collection tube according to claim 1, wherein the anticoagulant (5) is in the form of a layer or a pellet.
5. The blood collection tube according to claim 1, wherein the diameter of the immunomagnetic bead lyophilized microsphere (2) is 0.1-0.4 μm.
6. The blood collection tube according to any one of claims 1 to 5, wherein the number of the immunomagnetic bead lyophilized microspheres (2) is 1 to 5.
7. Holder for use with a blood collection cuvette according to any one of claims 1 to 6, wherein the cuvette (1) is detachably held by the holder (3), and wherein a magnet (4) is arranged in the holder (3), the magnet (4) being located directly below the cuvette (1).
8. The holder according to claim 7, characterized in that the holder (3) is provided with a square recess (7) on the inside, and the test tube (1) is placed in the square recess (7).
9. The bracket according to claim 8, wherein the magnet (4) is a square magnet embedded in the bracket (3) and located directly below the square groove (7).
10. Test tube assembly, characterized in that it comprises a holder (3), said holder (3) being equipped with a blood collection test tube according to any one of claims 1 to 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222117733.6U CN218411922U (en) | 2022-08-12 | 2022-08-12 | Blood sampling test tube, support and test tube assembly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222117733.6U CN218411922U (en) | 2022-08-12 | 2022-08-12 | Blood sampling test tube, support and test tube assembly |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218411922U true CN218411922U (en) | 2023-01-31 |
Family
ID=85025985
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222117733.6U Active CN218411922U (en) | 2022-08-12 | 2022-08-12 | Blood sampling test tube, support and test tube assembly |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218411922U (en) |
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2022
- 2022-08-12 CN CN202222117733.6U patent/CN218411922U/en active Active
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