CN218404233U - Double chemotaxis type micro-fluidic device for semen separation and detection - Google Patents
Double chemotaxis type micro-fluidic device for semen separation and detection Download PDFInfo
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- CN218404233U CN218404233U CN202221043787.6U CN202221043787U CN218404233U CN 218404233 U CN218404233 U CN 218404233U CN 202221043787 U CN202221043787 U CN 202221043787U CN 218404233 U CN218404233 U CN 218404233U
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- groove
- seminal fluid
- semen
- pdms
- microchannel
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- 210000000582 semen Anatomy 0.000 title claims abstract description 103
- 238000000926 separation method Methods 0.000 title claims abstract description 26
- 230000035605 chemotaxis Effects 0.000 title claims abstract description 16
- 238000001514 detection method Methods 0.000 title claims description 11
- 239000004205 dimethyl polysiloxane Substances 0.000 claims abstract description 43
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims abstract description 43
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims abstract description 43
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims abstract description 43
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims abstract description 43
- 238000010438 heat treatment Methods 0.000 claims abstract description 42
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 claims abstract description 26
- 229960003387 progesterone Drugs 0.000 claims abstract description 13
- 239000000186 progesterone Substances 0.000 claims abstract description 13
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 4
- 239000011521 glass Substances 0.000 claims abstract description 4
- 230000009977 dual effect Effects 0.000 claims description 7
- 238000004891 communication Methods 0.000 claims description 2
- 239000002975 chemoattractant Substances 0.000 claims 4
- 231100000527 sperm abnormality Toxicity 0.000 abstract description 4
- 239000003795 chemical substances by application Substances 0.000 description 11
- 238000000034 method Methods 0.000 description 9
- 230000003399 chemotactic effect Effects 0.000 description 7
- 229920000936 Agarose Polymers 0.000 description 6
- 108010010803 Gelatin Proteins 0.000 description 6
- 229920000159 gelatin Polymers 0.000 description 6
- 239000008273 gelatin Substances 0.000 description 6
- 235000019322 gelatine Nutrition 0.000 description 6
- 235000011852 gelatine desserts Nutrition 0.000 description 6
- 230000005012 migration Effects 0.000 description 5
- 238000013508 migration Methods 0.000 description 5
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- 230000008569 process Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000019100 sperm motility Effects 0.000 description 2
- 208000007466 Male Infertility Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 238000013021 overheating Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000005394 sealing glass Substances 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
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Abstract
The utility model relates to a seminal fluid separation technical field, concretely relates to two chemotaxis type micro-fluidic device for seminal fluid separation detects, including the base, the top of base is provided with the PDMS board, is provided with the heating unit that is used for terminal surface formation even temperature field under the PDMS board between base and the PDMS board, is provided with seminal fluid entrance slot, seminal fluid export groove on the PDMS board to and the microchannel, seminal fluid entrance slot, seminal fluid export groove, and the microchannel is located directly over the heating unit, through the microchannel intercommunication between seminal fluid entrance slot and the seminal fluid export groove, progesterone chemotaxis has still been placed on the seminal fluid export groove, and the up end of PDMS board still is provided with the sealed glass board that is used for sealed seminal fluid entrance slot, seminal fluid export groove and microchannel. The scheme can quickly separate normal sperms from abnormal sperms, and cannot influence the sperms.
Description
Technical Field
The utility model relates to a semen separation technical field, concretely relates to two chemotaxis type micro-fluidic devices for semen separation detects.
Background
The assisted reproduction technology is a direct method for solving infertility, sperm screening is a key step for determining in vitro fertilization results, and meanwhile, because the existing male infertility is more and more serious, for the male, how to screen normal sperm from semen is particularly important.
At present, sperm screening mainly comprises an upstream method and an ultracentrifugation method. Both of these methods rely on the motility of sperm for screening, and at the same time, they consume a lot of samples and time, and cannot form a uniform standard, especially centrifugation will damage sperm DNA.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a two chemotaxis type micro-fluidic device for semen separation detects, the realization that can be quick is to the separation of normal sperm and abnormal sperm to can not lead to the fact the influence to sperm self.
In order to achieve the above object, the technical scheme of the utility model provide a two chemotaxis type micro-fluidic device for semen separation detects, the on-line screen storage device comprises a base, the top of base is provided with the PDMS board, be provided with between base and the PDMS board and be used for the heating element of the terminal surface formation even temperature field under the PDMS board, be provided with semen inlet groove, semen export groove on the PDMS board to and the microchannel, semen inlet groove, semen export groove to and the microchannel is located directly over the heating element, through the microchannel intercommunication between semen inlet groove and the semen export groove, progesterone chemotactic agent has still been placed on the semen export groove, the up end of PDMS board still is provided with the sealed glass board that is used for sealed semen inlet groove, semen export groove and microchannel.
The technical principle and the technical effect of the scheme are as follows: in the prior art, when utilizing the temperature to separate the sperm, mostly mainly set up different temperatures in the both sides of seminal fluid business turn over groove, set up a temperature in the seminal fluid import groove promptly, set up another temperature again in seminal fluid export groove, make corresponding seminal fluid from the low removal to the temperature height of temperature like this to the realization is to the separation of seminal fluid.
According to the semen inseminating device, the sperm corresponding to the semen outlet groove is further attracted by the progesterone agent in the semen outlet groove when the semen moves towards the semen outlet groove, and therefore the separation of the normal sperm from the abnormal sperm is achieved.
This application passes through heating element and progesterone chemotaxis agent, realized the long-range migration of seminal fluid and the short-range migration that corresponds, promptly through the heating element, come to simulate the process that the seminal fluid enters into uterus, the migration of the longer stroke of corresponding seminal fluid has been realized, and through progesterone chemotaxis agent, further short-range migration to the seminal fluid, through these two kinds of migrations, make the process of seminal fluid separation more rapid and accurate, be convenient for later the development of the detection achievement of corresponding seminal fluid, through the aforesaid realized the quick separation to normal sperm and abnormal sperm, simultaneously through this kind of method, the separation that the characteristic of the sperm self of utilization realized, can not cause the influence to sperm self.
Furthermore, the heating unit comprises a controller, a selection key, a control key, a display and a plurality of heating modules, and the plurality of heating modules are uniformly arranged in the length direction of the microchannel; the controller is respectively electrically connected with the selection key, the control key, the display and the plurality of heating modules;
the controller is used for determining the heating modules of which the temperatures need to be adjusted through the selection keys, adjusting the temperatures of the heating modules through the control keys, and displaying the temperatures of the heating modules through the display.
Through the cooperation of selecting the button and controlling the button, can be so that the quick correspondence of operating personnel needs the heating module of adjustment temperature to adjust, can realize the repetitious usage of whole device like this, the utilization ratio of the device that improves greatly.
Further, the heating unit is embedded on the lower end face of the PDMS plate.
The heating unit is inlaid on the lower end face of the PDMS plate, so that the whole structure is more compact, the connection between the PDMS plate and the base is more firm, and meanwhile, the heating unit is tightly surrounded by the base and the PDMS plate, so that the environment corresponding to the heating unit is more closed, and the loss of heat of the heating unit is avoided.
Further, the PDMS plate was filled with an agarose/gelatin mixture.
The diffusion of the corresponding semen is made more rapid by the filling of the agarose/gelatin mixture.
Further, the micro-channel is a connecting groove arranged on the upper end face of the PDMS plate.
The setting of spread groove makes more convenience when handling the seminal fluid.
Further, the microchannel is a communication hole arranged in the PDMS plate.
The semen that avoids that the setting of intercommunicating pore can be better receives the pollution to and the sealed effect of the sealed glass board that corresponds can be better.
Furthermore, the connecting grooves comprise a first groove and a second groove, two ends of the first groove are respectively communicated with the semen outlet groove and the semen inlet groove, and the first groove and the second groove are perpendicularly crossed on the PDMS plate.
When seminal fluid migrates in first recess, when the second recess is passed through, can carry out an effective and quick judgement to the effect of heating element and progesterone chemotaxis agent through the motion condition of seminal fluid in the second recess, when whole device is used, the seminal fluid that observes moves along the length direction of first recess promptly that the whole flow direction of seminal fluid in the spread groove removes to the seminal fluid exit, and when in the second recess, if observe that the seminal fluid has obvious to the removal of second recess both sides, when leading to the unable normal removal to the seminal fluid exit of corresponding seminal fluid, then judge that corresponding device is useless.
The setting of second recess can be so that operating personnel when using the device, carries out a quick and accurate judgement to the quality of device, the later stage unnecessary step that has significantly reduced for the time that corresponds is consumed and separation efficiency's reduction.
Furthermore, the number of the second grooves is multiple, and the second grooves are uniformly arranged along the length direction of the first grooves.
Can judge at a plurality of positions through the even setting of a plurality of second recesses, carry out judgement many times to this comes to carry out reasonable more accurate judgement to the good or bad of whole device, avoids judging the mistake, thereby the extravagant problem appears.
Drawings
Fig. 1 is a schematic diagram of an overall structure of a dual-chemotaxis microfluidic device for semen separation and detection according to an embodiment of the present invention.
Fig. 2 is a cross-sectional view of a dual chemotaxis microfluidic device for semen separation detection in accordance with one embodiment of the present invention.
Detailed Description
The following is further detailed by way of specific embodiments:
the reference numbers in the drawings of the specification include: base 1, PDMS board 2, seminal fluid entry groove 3, seminal fluid export groove 4, first recess 5, second recess 6.
The first embodiment is as follows:
the first embodiment is substantially as shown in figures 1 and 2: the utility model provides a two chemotaxis type micro-fluidic device for semen separation detects, includes base 1, and the top of base 1 is provided with PDMS board 2, is provided with between base 1 and the PDMS board 2 to be used for the heating unit of PDMS board 2 bottom surface formation even temperature field, and in this embodiment, the heating unit is inlayed on PDMS board 2's bottom surface, and it can be more firm to fix between PDMS board 2 and the base 1 like this, and is concrete, gives the liquefaction with corresponding PDMS material, then pours the model through corresponding, arrives PDMS in the heating unit top for PDMS solidifies, thereby forms corresponding PDMS board 2. While the corresponding agarose/gelatin mixture is injected into the interior throughout the casting process, so that the agarose/gelatin mixture is cured together with the PDMS. In this example, the agarose/gelatin mixture ratio is 80%/20%, i.e. 80% agarose and 20% gelatin, which is more favorable for sperm motility, in which sperm motility is not particularly affected, and the corresponding temperature does not cause them to melt, make them too watery, and also promote the diffusion of progesterone chemotactic agent.
Be provided with semen entering groove 3, semen export groove 4 on PDMS board 2 to and the microchannel, semen entering groove 3, semen export groove 4, and the microchannel is located directly over heating unit, through the microchannel intercommunication between semen entering groove 3 and the semen export groove 4, the microchannel that corresponds in this embodiment is for setting up the spread groove on PDMS board 2 up end, the microchannel is the intercommunicating pore of setting in PDMS board 2 inside in another embodiment.
The corresponding connecting groove comprises a first groove 5 and a second groove 6, two ends of the first groove 5 are respectively communicated with the semen outlet groove 4 and the semen inlet groove, and the first groove 5 and the second groove 6 are perpendicularly crossed on the PDMS plate 2. Meanwhile, the number of the second grooves 6 is multiple and the second grooves are uniformly arranged along the length direction of the first groove 5. The number of second recesses 6 is 4 in this embodiment.
In this embodiment, the heating unit includes a controller, a selection key, a control key, a display, and a plurality of heating modules, and the plurality of heating modules are uniformly arranged along the length direction of the microchannel; the controller is respectively electrically connected with the selection key, the control key, the display and the plurality of heating modules;
the controller is used for determining the heating modules of which the temperatures need to be adjusted through the selection keys, adjusting the temperatures of the heating modules through the control keys, and displaying the temperatures of the heating modules through the display. In this embodiment, the corresponding controller is an STM 32-series single chip microcomputer, and the selection key and the control key are micro-touch keys.
The semen outlet groove 4 is also provided with a progesterone chemotactic agent, and in this embodiment, when the progesterone chemotactic agent is placed, the corresponding progesterone chemotactic agent is placed after the corresponding temperature gradient is kept stable.
The upper end face of the PDMS plate 2 is also provided with a sealing glass plate for sealing the semen inlet groove 3, the semen outlet groove 4 and the micro-channel.
The specific implementation process is as follows: in the use process, firstly, the heating units are communicated, the temperature corresponding to each heating module is adjusted by utilizing the selection keys, the temperature gradient required by people is achieved, after the corresponding temperature gradient is stabilized, a proper amount of progesterone chemotactic agent is dripped on the semen outlet groove, the early-stage preparation work is completed, then the corresponding semen is dripped on the semen inlet groove, the semen can move towards the semen outlet groove under the action of the temperature gradient, meanwhile, when the semen is close to the semen outlet groove, the corresponding progesterone chemotactic agent can attract the semen, the normal semen can flow towards the semen outlet groove, the abnormal semen is still near the semen inlet groove, and the separation of the semen is realized.
The above are only embodiments of the present invention, the present invention is not limited to the field related to this embodiment, the common general knowledge of the known specific structure and characteristics in the scheme is not described herein too much, and those skilled in the art know the common technical knowledge in the technical field of the present invention before the application date or the priority date, can know all the prior art in this field, and have the capability of applying the conventional experimental means before this date, and those skilled in the art can combine their own capability to perfect and implement the scheme, and some typical known structures or known methods should not become obstacles for those skilled in the art to implement the present application. It should be pointed out that, for the person skilled in the art, without departing from the structure of the invention, several variants and modifications can be made, which should also be regarded as the scope of protection of the invention, which will not affect the effectiveness of the implementation of the invention and the utility of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (7)
1. A two chemotaxis type micro-fluidic device for semen separation detects which characterized in that: the device comprises a base, the top of base is provided with the PDMS board, be provided with between base and the PDMS board and be used for the heating unit of the even temperature field of PDMS board downside formation, be provided with seminal fluid entry groove, seminal fluid export groove on the PDMS board to and the microchannel, seminal fluid entry groove, seminal fluid export groove, and the microchannel is located directly over the heating unit, through the microchannel intercommunication between seminal fluid entry groove and the seminal fluid export groove, progesterone chemotaxis has still been placed on the seminal fluid export groove, the up end of PDMS board still is provided with the sealed glass board that is used for sealed seminal fluid entry groove, seminal fluid export groove and microchannel.
2. The dual chemoattractant microfluidic device for semen separation detection of claim 1, wherein: the heating unit comprises a controller, a selection key, a control key, a display and a plurality of heating modules, and the plurality of heating modules are uniformly arranged in the length direction of the microchannel; the controller is respectively electrically connected with the selection key, the control key, the display and the plurality of heating modules;
the controller is used for determining the heating modules of which the temperatures need to be adjusted through the selection keys, adjusting the temperatures of the heating modules through the control keys, and displaying the temperatures of the heating modules through the display.
3. The dual chemoattractant microfluidic device for semen separation detection of claim 2, wherein: the heating unit is embedded on the lower end face of the PDMS plate.
4. The dual chemotaxis microfluidic device for semen separation detection of claim 3, wherein: the micro-channel is a connecting groove arranged on the upper end face of the PDMS plate.
5. The dual chemoattractant microfluidic device for semen separation detection of claim 3, wherein: the microchannel is a communication hole arranged in the PDMS plate.
6. The dual chemotaxis microfluidic device for semen separation detection of claim 4, wherein: the connecting groove comprises a first groove and a second groove, two ends of the first groove are respectively communicated with the semen outlet groove and the semen inlet groove, and the first groove and the second groove are perpendicularly crossed on the PDMS plate.
7. The dual chemoattractant microfluidic device for semen separation detection of claim 6, wherein: the quantity of second recess has a plurality ofly, evenly sets up along the length direction of first recess.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221043787.6U CN218404233U (en) | 2022-04-29 | 2022-04-29 | Double chemotaxis type micro-fluidic device for semen separation and detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221043787.6U CN218404233U (en) | 2022-04-29 | 2022-04-29 | Double chemotaxis type micro-fluidic device for semen separation and detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218404233U true CN218404233U (en) | 2023-01-31 |
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|---|---|---|---|
| CN202221043787.6U Active CN218404233U (en) | 2022-04-29 | 2022-04-29 | Double chemotaxis type micro-fluidic device for semen separation and detection |
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| CN (1) | CN218404233U (en) |
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2022
- 2022-04-29 CN CN202221043787.6U patent/CN218404233U/en active Active
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