CN218281804U - Liquid-transfering gun head - Google Patents

Abstract

The application provides a pipette head, including the suction nozzle of the pipette connector, columniform stock solution main part and the frustum shape of connecting in order, wherein, pipette connector, stock solution main part and suction nozzle three have common center pin, in the section face through the center pin, the opening edge of suction nozzle is first circular arc shape. The application of liquid-transfering gun head, because the opening edge of suction nozzle has convex cross-section, smooth no burr can reduce the damage that shearing force and tissue precision structure and mouth of pipe collision caused when using, the protection is absorb the integrality of object.

Description

Liquid-transfering gun head

Technical Field

The application relates to the field of biochemical experimental equipment, in particular to a liquid-transfering gun head.

Background

The conventional pipettor works by the expansion and contraction of a spring and needs to be matched with a pipette tip for use to suck thin or viscous liquid and cell tissues.

The field of stem cells has made breakthrough progress over the last decade, with one of the key advances being organoid differentiation and application. Organoids are 3D cell cultures, can possess spatial tissues and partial functions similar to those of corresponding organs, are used in physiological studies, disease model simulation, drug screening, and the like of different organs, and are a hotspot of research. Organoids are derived from pluripotent stem cells or embryonic stem cells and need to be cultured in vitro for a long time, including retinal organoids, brain organoids, intestinal organoids, and the like. For example, in the case of more retinal organoids, the mature retina can be obtained by culturing in a culture dish for more than 120 days, during which several transfers of different media and different types of culture dishes are required. The differentiation of retinal organs requires a significant time and expense for researchers, and is expensive. The retina organoids can grow out of the outer segment after being mature, are fine villi which are prominent on the surfaces of spherical organoids, are important phenotypes in disease simulation, are easily damaged by people in the transfer process, and influence the scientificity and objectivity of experimental results. At present, pipette tips in the market are small in caliber and are designed in a plane mode at the opening end, and the pipette tips which can protect organoid microstructures when not used are not very large in damage to precision structures, and waste of cost, instability of experimental results and objective scientific conclusion are caused.

Disclosure of Invention

In view of the above, the present application aims to provide a pipette tip suitable for aspirating biological tissue.

The liquid-transfering gun head of this application embodiment, including the suction nozzle of the liquid-transfering gun connector that connects in order, columniform stock solution main part and frustum shape, wherein, liquid-transfering gun connector, stock solution main part and suction nozzle three have common center pin, process in the section of the center pin, the opening edge of suction nozzle is first circular arc shape.

Optionally, a first arc angle corresponding to the first arc shape is 100 ° to 180 °.

Optionally, the method further comprises: the easy-to-break ring is located on the suction nozzle and parallel to the plane where the edge of the opening is located, the easy-to-break ring is broken to form a breaking fracture, and the breaking fracture is in a second arc shape in a cutting plane passing through the central shaft.

Optionally, a second arc angle corresponding to the second arc shape is 100 ° to 180 °.

Optionally, the pipette connector comprises an inward convex ring and a gun head outward protrusion, the inward convex ring can be matched with a connector of the pipette, and the gun head outward protrusion can be matched with a gun head hole of the gun head accommodating box.

Optionally, the distance between the plane of the opening and the plane of the easy-to-break ring is greater than 3mm.

Optionally, the length of the pipette connector is L1, and the value of L1 is 15 to 25mm; the length of the liquid storage main body is L2, and the value of L2 is 20-30 mm; the length of the suction nozzle is L3, and the value of L3 is 15-25 mm.

Optionally, the opening has a diameter of 2 to 4mm.

Optionally, the cone angle of the frustum-shaped nozzle is 15 ° to 30 °.

Optionally, the cone angle of the frustum-shaped nozzle is 18 °.

According to the technical scheme of this application, the opening of pipetting gun head's suction nozzle has convex cross-section, and is smooth no burr, can reduce the damage that shearing force and tissue precision structure and mouth of pipe collision caused when using, and the protection is absorb the integrality of object.

Drawings

The drawings are included to provide a further understanding of the application and are not to be construed as limiting the application. Wherein:

fig. 1 is a perspective view of a pipette tip according to an embodiment of the invention;

fig. 2 is a schematic perspective view of a pipette tip according to an embodiment of the present invention, in which a half of the pipette tip is cut along a central axis;

FIG. 3 is a schematic perspective view of another angle of the structure shown in FIG. 2;

FIG. 4 is an enlarged partial view of the contents of the box in FIG. 3;

FIG. 5 is a schematic view of a pipette tip according to an embodiment of the present invention after being broken from an easy-break ring position;

FIGS. 6 and 7 are a perspective and a perspective schematic view, respectively, of the upper half of the pipette tip of FIG. 5 after it has been broken away from the frangible ring position;

fig. 8 and 9 are a perspective and perspective schematic view, respectively, of the lower half of the pipette tip of fig. 5 broken away from the frangible ring position.

Detailed Description

The utility model discloses liquid-transfering gun head is as shown in fig. 1 to fig. 4, and this liquid-transfering gun head is including the suction nozzle 3 of liquid-transfering gun connector 1, columniform stock solution main part 2 and frustum shape that connect in order. Wherein, the pipette tip 1, the liquid storage body 2 and the suction nozzle 3 have a common central axis, and in a section plane passing through the central axis, the edge of the opening 31 of the suction nozzle 3 is in a first arc shape (see position a in fig. 4).

According to the utility model discloses a liquid-transfering gun head, because suction nozzle opening edge has convex cross-section, smooth no burr can reduce the damage that shearing force and tissue precision structure and mouth of pipe collision caused when using, the integrality of protection absorbed object.

Preferably, the first arc angle (see the angle α corresponding to the position a in fig. 4) corresponding to the first arc shape is 100 ° to 180 °, and the arc shape in the angle range is relatively smooth and easy to process, and is more suitable for absorbing a precise tissue structure and protecting an absorbed object from being damaged.

The utility model discloses liquid-transfering gun head can also include: and the easy-folding ring 4 is positioned on the suction nozzle 3 and is parallel to the plane of the edge of the opening 31, and the easy-folding ring 4 is broken to form a breaking fracture. Wherein the fracture has a second circular arc shape in a cross-sectional plane passing through the central axis (see B-position in fig. 4).

Preferably, the second arc angle (see the angle β corresponding to the position B in fig. 4) corresponding to the second arc shape is 100 ° to 180 °, and the arc shape in the angle range is relatively smooth and easy to process, and is more suitable for absorbing the precise structure of the tissue and protecting the absorbed object from being damaged.

The frangible ring 4 can facilitate the user breaking the mouthpiece into two parts. The user may then discard the portion having the original opening 31. At this moment, smooth burr-free and the bigger destruction fracture of diameter has been exposed to the position that easy rupture ring 4 of pipette tip originally was located, and this destruction fracture can supply the user to use as new opening, is applicable to the object of absorbing the volume great or viscosity great. For example, as shown in fig. 5, the user can apply a force on the frangible ring 4 such that the nozzle 3 of the pipette tip is divided into a lower nozzle portion 32 and an upper nozzle portion 33, thereby dividing the pipette tip into upper and lower portions, the details of which can be seen with further reference to fig. 6-9.

The utility model discloses in the liquid-transfering gun head, liquid-transfering gun connector 1 is connected with liquid-transfering gun detachably when using. The pipette connector 1 may include an inwardly projecting annular ring 11 and a pipette tip projection 12. The inward convex ring 11 can ensure that the connection between the inward convex ring and the pipette tip is firmer and the pipette tip is not easy to fall off. Rifle head evagination 12 can match the rifle head hole that the rifle head held the box for guarantee that the liquid-transfering gun head is placed steadily in the rifle head holds the box before starting.

In order to avoid too small difference between the diameters of the opening and the breakable ring and to facilitate the application of force during breaking, the distance between the plane of the opening 31 and the plane of the breakable ring 4 should be greater than 3mm.

In the pipette head of the embodiment of the utility model, the length L1 value of the pipette head 1 can be 15-25 mm, preferably 20mm; the length L2 of the reservoir body 2 may be 20 to 30mm, preferably 25mm; the length L3 of the suction nozzle 3 can take the value of 15 to 25mm, preferably 20mm; the diameter of the opening 31 may be 2 to 4mm, preferably 3mm; the cone angle of the frustum-shaped suction nozzle 3 can be 15 ° to 30 °, preferably 18 °.

In conclusion, the pipette tip of the application is suitable for organoids to be used in culture and various experimental scenes, for example, immunofluorescence staining, electron microscope scanning, live cell imaging and the like need to be carried out on the transfer of different culture dishes.

The liquid-transfering gun head of this application embodiment can reduce the shearing force when using and to the destruction of cell and tissue, and the damage when reducing the accurate micro-structure of tissue and the mouth of pipe collision when absorbing can be used for viscous liquid's absorption simultaneously, like tween, triton, glycerine etc.. In addition, due to the arrangement of the easy-breaking ring which is easy to break, the product can meet the use requirements of objects with different sizes when being sucked.

The above-described embodiments should not be construed as limiting the scope of the present application. Those skilled in the art will appreciate that various modifications, combinations, sub-combinations, and substitutions can occur, depending on design requirements and other factors. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims (10)

1. The pipette tip is characterized by comprising a pipette tip connector, a cylindrical liquid storage main body and a frustum-shaped suction nozzle which are sequentially connected, wherein the pipette tip connector, the liquid storage main body and the suction nozzle are provided with a common central shaft, and the opening edge of the suction nozzle is in a first arc shape in a cutting plane passing through the central shaft.

2. A pipette tip according to claim 1, characterised in that the first arc shape corresponds to a first arc angle of 100 ° to 180 °.

3. A pipette tip according to claim 1, and further comprising: an easy-to-break ring on the mouthpiece parallel to the plane of the opening edge, the easy-to-break ring being broken to form a breaking break, wherein,

in a section plane passing through the central axis, the fracture is in a second arc shape.

4. A pipette tip according to claim 3, characterised in that the second arc shape corresponds to a second arc angle of 100 ° to 180 °.

5. The pipette tip according to claim 1, wherein the pipette tip connector comprises an inward protruding ring and a tip protrusion, the inward protruding ring can match a port of the pipette tip, and the tip protrusion can match a tip hole of the tip accommodating box.

6. A pipette tip according to claim 3, characterised in that the distance between the plane of the opening and the plane of the frangible ring is greater than 3mm.

7. The pipette tip according to any one of claims 1 to 5, wherein the length of the pipette tip connector is L1, and the value of L1 is 15 to 25mm; the length of the liquid storage main body is L2, and the value of L2 is 20-30 mm; the length of the suction nozzle is L3, and the value of L3 is 15-25 mm.

8. A pipette tip according to any of claims 1 to 5, characterised in that the diameter of the opening is 2 to 4mm.

9. A pipette tip according to any of claims 1 to 5, characterised in that the cone angle of the frustum-shaped suction nozzle is from 15 ° to 30 °.

10. A pipette tip according to any of claims 1 to 5, characterised in that the cone angle of the frustum-shaped suction nozzle is 18 °.

Images