CN218262492U

CN218262492U - Sample storage tube for automatic nucleic acid extraction equipment

Info

Publication number: CN218262492U
Application number: CN202120046148.4U
Authority: CN
Inventors: 居金良; 金浩; 赵玉军; 曹春鹏
Original assignee: Shanghai Rendu Biotechnology Co ltd
Current assignee: Shanghai Rendu Biotechnology Co ltd
Priority date: 2021-01-08
Filing date: 2021-01-08
Publication date: 2023-01-10
Anticipated expiration: 2031-01-08

Abstract

The utility model discloses a sample storage tube for automatic nucleic acid extraction equipment, which aims at the problems that the sample tube of the current automatic nucleic acid extraction equipment needs manual opening, wastes time and labor and easily causes sample sprinkling and pollution, by designing the tube cap of the sample tube into a hollow body, the inner wall of the tube cap is fixedly provided with an annular limiting leak-proof piece, and a hot pressing film is arranged on the annular limiting leak-proof piece and is used for sealing the upper opening of the tube cap; the lower part of the inner wall of the pipe cap is provided with threads which are matched with the external threads at the opening end of the pipe body; when the pipe body is connected with the pipe cap, the annular limiting leak-proof piece gradually contacts the opening end of the sealing pipe body; when sampling the sample, the suction head can directly pierce the hot pressing film to take the liquid. Effectively prevent the sample and leak, and need not artifical uncapping, reduce artifical the participation, improve the automation process of nucleic acid extraction, effectively prevent to pollute and reduce and detect the failure rate.

Description

Sample storage tube for automatic nucleic acid extraction equipment

Technical Field

The utility model belongs to the technical field of automatic nucleic acid extraction, especially, relate to a sample preservation pipe for automatic nucleic acid extraction equipment.

Background

In the molecular diagnosis and detection process, a sample of a target group is collected and collected firstly, and the sample collection is to collect body fluid of the target group, such as blood, saliva, urine, semen and the like, preserve and transfer the body fluid into a specific sample tube for preservation, storage and detection. The sample from collection to detection involves the preservation of the sample and the disposal against contamination.

The sample tube used by the automated nucleic acid extraction apparatus on the market at present is the same as the sample tube of the manual extraction operation, and can be divided into two types according to the way of closing the tube orifice: one is close-fitting direct-pressure sealing of the sample tube, and the sealed sample tube is forcibly shifted up and down by a manual finger; the other type is a sample tube sealed by screw threads, and the sample tube is opened by rotating the left and right hands of the artificial finger; all need manually open the sample tube cap when putting into automatic nucleic acid extraction equipment, consuming time and wasting power to can appear artifical error and cause the sample unrestrained, the condition such as pollution.

One method is to seal a film on the top of a sample tube cap, screw down the tube cap after collecting a sample tube, store the sample tube, directly transfer the sample tube cap to an automatic instrument without manually screwing down the sample tube cap, directly break a hole and sample by the automatic instrument, reduce manual interference and improve efficiency; because of sealing membrane material, intensity and broken hole mode and broken hole time occupy whole equipment operating duration extension and reduce extraction efficiency and increase cost, more importantly this kind of mode of sample tube membrane sealing broken hole use in the market at present not very ripe, cause the broken hole easily and get liquid unable accurate liquid of getting because of the intraductal and outer pressure differential of sample that the jam formed when getting liquid.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a sample storage tube for automizing nucleic acid extraction equipment directly puts into automizing nucleic acid extraction equipment, need not artifical uncapping, reduces artifical the participation, improves the automation process of nucleic acid extraction, effectively prevents to pollute and reduces and detects the failure rate.

In order to solve the above problem, the technical scheme of the utility model is that:

a sample storage tube for an automatic nucleic acid extraction device comprises a tube body and a tube cap, wherein the tube body is in threaded connection with the tube cap;

the pipe cap is a hollow body, an annular limiting leak-proof piece is fixedly arranged on the inner wall of the pipe cap, a hot pressing film is arranged on the annular limiting leak-proof piece, and the upper opening of the pipe cap is sealed by the hot pressing film; the lower part of the inner wall of the pipe cap is provided with threads, and the threads are matched with the external threads at the opening end of the pipe body;

when the pipe body is connected with the pipe cap, the lower end face of the annular limiting leakage-proof piece extrudes the opening end of the pipe body to form sealing.

According to the utility model relates to an embodiment, hot pressing membrane includes epiphragma and lower floor's membrane, the epiphragma easily pierces through, the lower floor's membrane with the spacing leak protection of annular is easily laminated.

According to the utility model discloses an embodiment, the upper strata rete is the fragile aluminium membrane, the underlayer film is the plastic film.

According to the utility model relates to an embodiment, be equipped with the puncture position of ventilating of fretwork and the liquid of getting of fretwork puncture position on the lower membrane.

According to an embodiment of the present invention, the ventilation puncture site or the liquid-extraction puncture site is circular.

According to the utility model discloses an embodiment, the puncture position of ventilating and the puncture position intercommunication of getting liquid to be spindle-shaped.

According to the utility model discloses an embodiment, the spacing leak protection of annular is located the middle part of pipe cap inner wall, hot pressing membrane indent in the pipe cap.

According to the utility model relates to an embodiment, the body is cylindrically, the external diameter of body and the position matching is placed with the sample of automatic nucleic acid extraction equipment to the height.

The utility model discloses owing to adopt above technical scheme, make it compare with prior art and have following advantage and positive effect:

1) The utility model discloses a sample storage tube for automatic nucleic acid extraction equipment in one embodiment, to the sample tube of present automatic nucleic acid extraction equipment need manual to open, waste time and energy and easily cause the problem that the sample spills, pollutes, through designing the pipe cap of sample tube into the hollow body, the inner wall of pipe cap sets firmly an annular spacing leak protection piece, is equipped with the hotpressing membrane on this annular spacing leak protection piece and is used for the upper portion opening of sealed pipe cap; the lower part of the inner wall of the pipe cap is provided with threads which are matched with the external threads at the opening end of the pipe body; when the pipe body is connected with the pipe cap, the annular limiting leak-proof piece gradually contacts the opening end of the sealing pipe body; the hot pressing film can be directly punctured by the sucker to take liquid during sampling. Effectively prevent that the sample from leaking, and need not artifical uncapping, reduce artifical the participation, improve the automation process of nucleic acid extraction, effectively prevent to pollute and reduce and detect the failure rate.

2) The sample storage tube for the automatic nucleic acid extraction equipment in one embodiment of the utility model can extrude the open end of the tube body through the annular limiting leak-proof piece when the tube cap is connected with the tube body, on one hand, the sealing is formed from the side surface, and the sample side leakage is prevented; on the other hand, play limiting displacement, prevent that the body from excessively deepening the pipe cap, causing the damage of hot pressing membrane.

3) The utility model discloses a sample storage tube for automizing nucleic acid and draw equipment in an embodiment adopts fragile aluminium membrane as the upper strata membrane of hot pressing membrane, just can impale with very little power when the puncture, makes things convenient for the sample.

4) The utility model discloses a sample storage tube for automatic nucleic acid extraction equipment in an embodiment sets up the puncture position of ventilating of fretwork and gets liquid puncture position on the lower layer membrane of hot pressing membrane, effectively prevents to get the in-process of liquid at broken hole, can't accurately get liquid because of the inside and outside pressure differential of the sample pipe that the jam formed.

5) The utility model discloses a sample preservation tube for automizing nucleic acid and draw equipment in an embodiment, the design that the hot pressing membrane is concave in the pipe cap can make the hot pressing membrane protected by the pipe cap, prevents effectively that the sample pipe from causing the damage of hot pressing membrane because of the collision in transportation, storage process.

Drawings

FIG. 1 is a schematic view of a cap for use in a sample storage tube of an automated nucleic acid extraction apparatus according to an embodiment of the present invention;

fig. 2 is a schematic view of a hot pressing film according to an embodiment of the present invention;

fig. 3 is a schematic view of an upper film in a hot-pressed film according to an embodiment of the present invention;

fig. 4 is a schematic view of an underlying film in a hot-pressed film according to an embodiment of the present invention;

fig. 5 is a schematic view of a tube body in a sample storage tube for an automated nucleic acid extraction apparatus according to an embodiment of the present invention.

Description of reference numerals:

1: a pipe cap; 11: an upper layer of film; 12: a lower layer film; 13: a thread; 14: an annular limiting anti-leakage piece; 2: a tube body; 21: volume scales; 22: an external thread; a: a ventilating puncture site; b: taking liquid and puncturing the position.

Detailed Description

The following provides a sample storage tube for an automated nucleic acid extraction device, which is provided in the present invention, with reference to the accompanying drawings and specific embodiments. The advantages and features of the present invention will become more fully apparent from the following description and appended claims.

The embodiment provides a sample storage tube for an automated nucleic acid extraction device, please refer to fig. 1 and 5, the sample storage tube comprises a tube body 2 and a tube cap 1, and the tube body 2 is connected with the tube cap 1 by screw threads.

Specifically, the cap 1 is a hollow body, and has a cylindrical shape. The inner wall of the pipe cap 1 is fixedly provided with an annular limiting leak-proof piece 14, and a hot pressing film is arranged on the annular limiting leak-proof piece 14 and used for sealing the upper opening of the pipe cap 1. The lower part of the inner wall of the pipe cap 1 is provided with threads 13, and the threads 13 are matched with external threads 22 at the opening end of the pipe body 2. When the pipe body 2 is connected with the pipe cap 1, the lower end face of the annular limiting leak-proof piece 14 extrudes the opening end of the pipe body 2 to form sealing.

Wherein, the spacing leak protection of annular 14 locates the middle part of pipe cap 1 inner wall, and hot pressing membrane indent is in pipe cap 1, can make hot pressing membrane protected by the pipe cap like this, effectively prevents that the sample pipe from causing the damage of hot pressing membrane because of the collision in transportation, storage process. Of course, the annular limiting leak-proof member 14 may also be disposed on the upper portion of the inner wall of the cap 1, as long as the hot-pressed film is not flush with the open end of the cap 1.

Referring to fig. 2, 3 and 4, the hot-pressed film includes an upper layer 11 and a lower layer 12. The upper membrane 11 is a readily pierceable membrane such as brittle aluminium. The underlying film 12 is a film, such as a plastic film, that is easily conformable to the annular restraining leak-proof element 14. The fragile aluminum film is used as the upper layer film 11 of the hot pressing film, so that the film can be punctured by small force during liquid taking and puncturing, and sampling is facilitated. And the film which is easy to be jointed with the annular limiting leakage-proof piece 14 is adopted as the lower layer film 12, so that the binding force of the annular limiting leakage-proof piece 14 is strong during hot pressing, and the sealing property is good so as to prevent the sample liquid from leaking.

The lower layer film 12 is provided with a hollow ventilation puncture site A and a hollow liquid taking puncture site B. The ventilation puncture site A or the liquid taking puncture site B can be in a circular shape, a square shape, a star shape or other shapes and is distributed on the lower layer film 12, so that the hollow part is prevented from being blocked due to burst caused by brittleness of the aluminum film when the aluminum film is punctured. The air-permeable puncture site A and the liquid-taking puncture site B can also be communicated and are spindle-shaped and positioned in the middle of the lower layer film 12. Through setting up the puncture position A of ventilating of fretwork and getting liquid puncture position B, effectively prevent at the broken hole in-process of getting liquid, can't get liquid because of the sample tube internal and external pressure differential that the jam formed.

The sample preservation pipe that this embodiment provided is used for automatic nucleic acid extraction equipment, and position cooperation is placed to its

cap

1 and 2 external diameters of body and height and the sample of automatic nucleic acid extraction equipment, and the sample position of the automatic nucleic acid extraction equipment of different structures is suitable for the sample preservation pipe of different volume capacity.

In addition, in order to avoid interference to take liquid after the test paper swabs for collecting mucus, excrement and the like are placed in the sample tube body, the depth and the width of the annular groove at the lower part of the tube cap 1 can be increased, and the test paper swabs are used for fixing the rear ends of the test paper swabs at the edge of the sample tube.

The operation of the sample storage tube of the present embodiment for use in an automated nucleic acid extraction apparatus is briefly described as follows:

the collected sample is collected in the tube body 2, the volume scale 21 on the side of the tube body 2 is observed according to the predetermined amount of the collected sample, and the tube cap 1 is screwed after the predetermined amount of the collected sample is completed. When extracting and purifying, the sample storage tube can be directly placed into an automatic nucleic acid extraction device, and the automatic nucleic acid extraction device firstly punctures the air-permeable puncture site A and then punctures the liquid-taking puncture site B for sampling.

The sample storage tube for the automatic nucleic acid extraction equipment provided by the utility model has the advantages of simple structure, easy manufacture and low cost; need not artificially uncapping, practice thrift the manual work, improve the rate of automation.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the embodiments. Even if various changes are made to the present invention, the changes are still within the scope of the present invention if they fall within the scope of the claims and their equivalents.

Claims

1. A sample storage tube for an automatic nucleic acid extraction device comprises a tube body and a tube cap, wherein the tube body is in threaded connection with the tube cap; it is characterized in that the preparation method is characterized in that,

the pipe cap is a hollow body, an annular limiting leak-proof piece is fixedly arranged on the inner wall of the pipe cap, a hot pressing film is arranged on the annular limiting leak-proof piece, and the upper opening of the pipe cap is sealed by the hot pressing film; the lower part of the inner wall of the pipe cap is provided with a thread which is matched with an external thread at the opening end of the pipe body;

2. The sample storage tube for an automated nucleic acid extraction apparatus according to claim 1, wherein the heat pressing film comprises an upper film and a lower film, the upper film is easy to pierce, and the lower film is easy to attach to the annular position-limiting leakage-preventing member.

3. The sample storage tube for an automated nucleic acid extraction apparatus according to claim 2, wherein the upper film is a brittle aluminum film and the lower film is a plastic film.

4. The sample storage tube for an automated nucleic acid extraction apparatus according to claim 2 or 3, wherein the lower film is provided with a hollow-out air-vent puncture site and a hollow-out liquid-extraction puncture site.

5. The sample storage tube for an automated nucleic acid extraction apparatus of claim 4, wherein the vent puncture site or the draw puncture site is circular.

6. The sample storage tube for an automated nucleic acid extraction apparatus according to claim 4, wherein the vent puncture site is in communication with the access puncture site and has a spindle shape.

7. The sample storage tube for an automated nucleic acid extraction apparatus according to claim 1, wherein the annular position-limiting leak-proof member is disposed in a middle portion of an inner wall of the cap, and the hot-pressing film is recessed in the cap.

8. The sample storage tube for an automated nucleic acid extraction apparatus according to claim 1, wherein the tube body has a cylindrical shape, and an outer diameter and a height of the tube body match with a sample placement position of the automated nucleic acid extraction apparatus.