CN218250303U

CN218250303U - Micro-channel structure of micro-fluidic chip

Info

Publication number: CN218250303U
Application number: CN202220437328.XU
Authority: CN
Inventors: 付文成; 李思斌
Original assignee: Yihong Shenzhen Gene Co ltd
Current assignee: Yihong Shenzhen Gene Co ltd
Priority date: 2022-03-02
Filing date: 2022-03-02
Publication date: 2023-01-10
Anticipated expiration: 2032-03-02

Abstract

A micro-channel structure of a micro-fluidic chip comprises an upper plate and a lower plate, wherein a micro-channel forming support is arranged on the lower plate, a closed notch is formed in the micro-channel forming support, and the upper plate and the lower plate are connected to form a micro-channel. Because the lower plate is provided with the micro-channel forming support with the sealing gap, the upper plate and the lower plate can be connected to form the micro-channel, when the micro-channel needs to be sealed, the upper plate is melted or pressed to be attached to the lower plate at the sealing gap, and the sealing of the micro-channel can be completed.

Description

Micro-channel structure of micro-fluidic chip

Technical Field

The application relates to the technical field of micro-fluidic chips, in particular to a micro-channel structure of a micro-fluidic chip.

Background

Microfluidics (Microfluidics) refers to the science and technology involved in systems that process or manipulate tiny fluids using microchannels, and microfluidic devices are commonly referred to as microfluidic chips, also known as lab-on-a-chip.

The micro flow channel is an essential infrastructure for achieving microfluidic control. The smooth formation and the reclosing of the micro-flow channel are key technologies for realizing micro-flow control, and various enterprise organizations have different technical routes for the micro-flow control. Common methods include injection molding, gluing, ultrasonic welding, laser engraving, and the like; the micro flow channel sealing technology includes micro valve method, rubber plug method, mechanical pressing and other methods. However, these methods have problems of complicated structure, high cost, interfering with the properties of the fluid, and the like, and have not been widely used so far.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the application provides a micro-channel structure of a micro-fluidic chip.

According to a first aspect of the present application, there is provided in one embodiment a micro flow channel structure of a micro flow chip, including:

an upper plate;

the lower plate is provided with a micro-channel forming support;

a closed gap is arranged on the micro-channel forming support;

the upper plate and the lower plate are connected to form a micro-channel.

In one embodiment, the upper plate is a film or flexible sheet made of a lower melting point material; the lower plate is made of hard plastic, is stable in physical and chemical properties, is not easy to melt and has certain mechanical strength.

In one embodiment, the upper plate and the lower plate are connected by heat melting or gluing.

In one embodiment, the microchannel forming support pattern comprises a dotted line pattern, an open pattern, or a discontinuous curve pattern.

In one embodiment, a liquid storage chamber is disposed on the lower plate, and the liquid storage chamber is communicated with the micro flow channel.

In one embodiment, the lower plate is provided with a reaction hole, and the reaction hole is communicated with the micro flow channel.

In one embodiment, a PCR reagent ball is arranged in the reaction hole, the inner layer of the PCR reagent ball is reagent dry powder required by PCR reaction, and the outer layer of the embedding object is low-melting-point inert solid.

In one embodiment, the outer layer embedding substance of the PCR reagent ball comprises paraffin, alkyl or long-chain alkane simple substance or mixture.

In one embodiment, the lower plate is provided with a plurality of liquid storage chambers or reaction holes, and the upper plate and the lower plate are connected to form a plurality of micro channels communicated with the liquid storage chambers or the reaction holes.

In one embodiment, the microchannels are connected in series or in parallel with the reservoir chamber or the reaction well.

According to the micro-channel structure of the micro-fluidic chip, the lower plate is provided with the micro-channel forming support with the sealing gap, so that the upper plate and the lower plate are connected to form the micro-channel, when the micro-channel needs to be sealed, the upper plate is melted or pressed and attached to the lower plate at the sealing gap, and the sealing of the micro-channel can be completed.

Drawings

FIG. 1 is a schematic diagram showing a structure of a micro flow channel of a microfluidic chip according to an embodiment;

FIG. 2 is an exploded view of a micro flow channel structure of a micro flow chip according to an embodiment;

FIG. 3 is a schematic view showing the structure of the lower plate of the micro flow channel structure of the micro flow control chip in one embodiment;

FIG. 4 is a schematic structural view of a front upper plate of a micro flow channel structure of a micro flow chip in a thermal fusion bonding manner according to an embodiment;

FIG. 5 is a schematic diagram of a structure of a top plate after a micro channel is closed in a micro channel structure of a micro fluidic chip according to an embodiment.

Description of reference numerals: 1. an upper plate; 2. a lower plate; 21. forming and supporting the micro-channel; 22. sealing the gap; 3. a micro flow channel; 4. a reaction well; 5. and (4) PCR reagent balls.

Detailed Description

The present application will be described in further detail below with reference to the accompanying drawings by way of specific embodiments. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments, and the operation steps involved in the embodiments may be interchanged or modified in order as will be apparent to those skilled in the art. Accordingly, the description and drawings are merely for clarity of description of certain embodiments and are not intended to necessarily refer to a required composition and/or order.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

The micro-channel structure of the micro-fluidic chip in the embodiment of the application comprises an upper plate and a lower plate, wherein a micro-channel forming support is arranged on the lower plate, a closed notch is arranged on the micro-channel forming support, and the upper plate and the lower plate are connected to form a micro-channel. Because be equipped with the microchannel shaping of taking the closed gap on the hypoplastron and support for the upper plate is connected with the hypoplastron and can be formed the microchannel, and when the microchannel need be sealed, melts the upper plate or press the laminating to the hypoplastron in closed breach department and can accomplish the closure of microchannel, and the formation and the closed step of microchannel are simple, and upper plate and hypoplastron simple structure, and low cost, and can not disturb fluid nature.

The present application is illustrated by the following specific examples.

The first embodiment is as follows:

as shown in fig. 1 to 5, an embodiment of the present application provides a micro channel structure of a micro fluidic chip, including: an upper plate 1 and a lower plate 2. The lower plate 2 is provided with a micro-channel forming support 21, and the micro-channel forming support 21 is provided with a closed gap 22. The upper plate 1 and the lower plate 2 are connected to form a microchannel 3. Because the lower plate 2 is provided with the micro-channel forming support 21, when the upper plate 1 is attached to the lower plate 2, part of the area of the upper plate 1 is supported by the micro-channel forming support 21, the supported part of the upper plate 1 and the micro-channel forming support 21 are semi-sealed to form the micro-channel 3, and when the micro-channel 3 needs to be sealed, the upper plate 1 is melted or pressed to be attached to the lower plate 2 at the sealing gap 22, so that the sealing of the micro-channel 3 can be completed.

In this embodiment, in order to make the connection effect between the upper plate 1 and the lower plate 2 better, the upper plate 1 is made of a film or a flexible thin plate made of a material with a lower melting point, such as PE, PET, PVC, etc.; the lower plate 2 is made of hard plastic, is stable in physical and chemical properties, not easy to melt and has certain mechanical strength, such as ABS, PC, PP and the like, and is matched with proper pressure when the upper plate 1 and the lower plate 2 are connected, and the lower plate 2 is provided with the flow channel forming support 21, so that the upper plate 1 can be tightly attached to the flow channel forming support 21, the tight sealing of the micro flow channel 3 can be realized, the book and the air cannot leak, and the sealing performance is good.

The micro flow channel forming support 21 may be configured in various forms such as a dotted line type, a cut-off type, a discontinuous curve type, etc. according to different requirements or conditions, and may realize the micro flow channel supporting function and leave a closed gap, which is not limited herein.

In this embodiment, the reaction hole 4 is formed in the lower plate 2, the micro flow channel 3 is communicated with the reaction hole 4 to feed liquid into the reaction hole 4, and the upper plate 1 can be melted or pressed and attached to the lower plate 2 at the sealing gap 22 after the feed liquid into the reaction hole 4 is completed, so as to complete the sealing of the micro flow channel 3.

In this embodiment, the reaction well 4 is provided with a PCR reagent ball 5, the inner layer of the PCR reagent ball 5 is reagent dry powder required for PCR reaction, and the outer layer of the embedded material is inert solid with low melting point, such as paraffin, alkyl, long-chain alkane simple substance or mixture, and other inert organic substances with melting point of 40-100 ℃. When PCR reaction is carried out, the embedded object on the outer layer of the PCR reagent ball 5 is heated and melted, and the PCR reaction reagent is released, so that the PCR reaction is finished.

It will be appreciated by those skilled in the art that the lower plate 2 may also be provided with chambers for performing other functions, such as reservoir chambers, with which the microchannels 3 communicate to effect the inlet of liquid. The lower plate 2 can be also provided with a plurality of liquid storage chambers and reaction holes 4 or chambers for realizing other functions, the chambers can be independently communicated with the micro-channel 3, the chambers can be connected in series or in parallel through the micro-channel 3, after liquid inlet of each chamber is completed, the upper plate 1 is melted or pressed and attached to the lower plate 2 at the sealing gap 22, so that sealing of each part of the micro-channel 3 can be completed, and independent PCR reaction conditions of each chamber are ensured.

According to the micro-channel structure of the micro-fluidic chip in the above embodiment, the micro-channel structure includes an upper plate and a lower plate, the lower plate is provided with a micro-channel forming support, the micro-channel forming support is provided with a closed gap, and the upper plate and the lower plate are connected to form a micro-channel. Because be equipped with the microchannel shaping of taking the closure gap on the hypoplastron and support for the upper plate can form the microchannel with hypoplastron connection, when the microchannel needs to seal, melt the upper plate or press the laminating to the hypoplastron in closure gap department and can accomplish the closure of microchannel, the formation and the closure step of microchannel are simple, upper plate and hypoplastron simple structure, low cost, be convenient for miniaturization and automatic volume production, and can not disturb fluid property.

It is right to have used specific individual example above the utility model discloses expound, only be used for helping to understand the utility model discloses, not be used for the restriction the utility model discloses. To the technical field of the utility model technical personnel, the foundation the utility model discloses an idea can also be made a plurality of simple deductions, warp or replacement.

Claims

1. A micro flow channel structure of a micro flow control chip is characterized by comprising:

an upper plate;

the lower plate is provided with a micro-channel forming support;

a closed gap is arranged on the micro-channel forming support;

the upper plate and the lower plate are connected to form a micro-channel.

2. The micro flow channel structure of a microfluidic chip according to claim 1, wherein the upper plate and the lower plate are connected by thermal fusion or adhesive.

3. The micro flow channel structure of a microfluidic chip according to claim 2, wherein the micro flow channel formation support form includes a dotted line type, an open type or a discontinuous curve type.

4. The micro flow channel structure of a microfluidic chip according to claim 3, wherein the lower plate is provided with a reservoir chamber, and the reservoir chamber is communicated with the micro flow channel.

5. The micro flow channel structure of a micro flow control chip according to claim 3, wherein the lower plate is provided with reaction holes, and the reaction holes are communicated with the micro flow channel.

6. The micro flow channel structure of a micro flow control chip according to claim 5, wherein PCR reagent balls are provided in the reaction wells.

7. The micro flow channel structure of a microfluidic chip according to claim 3, wherein the lower plate has a plurality of liquid storage chambers or reaction wells, and the upper plate and the lower plate are connected to form a plurality of micro flow channels communicating with the liquid storage chambers or the reaction wells.

8. The micro flow channel structure of a microfluidic chip according to claim 7, wherein the micro flow channel is connected in series or in parallel to the reservoir chamber or the reaction well.