CN218218932U - Closed freezing pole that carries - Google Patents
Closed freezing pole that carries Download PDFInfo
- Publication number
- CN218218932U CN218218932U CN202221877304.2U CN202221877304U CN218218932U CN 218218932 U CN218218932 U CN 218218932U CN 202221877304 U CN202221877304 U CN 202221877304U CN 218218932 U CN218218932 U CN 218218932U
- Authority
- CN
- China
- Prior art keywords
- sleeve
- closed
- length
- radial cross
- sectional dimension
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The application discloses pole is carried in closed freezing, includes: a carrier rod and a sleeve; the carrying rod comprises a connecting part, a handheld part and a carrying piece part which are respectively arranged at two ends of the connecting part; the connecting part comprises a connecting upper part, a connecting middle part and a connecting lower part, and the connecting lower part is connected with the carrying piece part; the sleeve comprises an outer sleeve and a weight piece; the outer sleeve is divided into an upper sleeve part, a middle sleeve part and a lower sleeve part, and the counterweight is fixedly arranged in the lower sleeve part; the cross-sectional dimension of the upper part of the sleeve is larger than the radial cross-sectional dimension of the middle part of the sleeve, and the radial cross-sectional dimension of the middle part of the sleeve is matched with the radial cross-sectional dimension of the connecting middle part. This application is through carrying the pole spacing in the sleeve, has solved prior art's inserted bar and slide glass combination and has inserted not have the strong point in the overcoat pipe, leads to embryo or cell on the slide glass directly to strike on the metal easily, the technical problem who causes embryo or cell to drop and damage of existence.
Description
Technical Field
The application relates to the technical field of biological reproduction, in particular to a closed type freezing carrying rod and an operation method.
Background
Biological samples, especially tissue samples, play an important role in the study of various diseases. With the development of precise medicine, the importance of tissue samples is more prominent. Therefore, the construction of a biological sample library is paid much attention, and the low-temperature cryopreservation technology of the tissue sample becomes a research hotspot. After the isolated tissue is frozen, whether the histiocyte can still keep good activity and function has important significance for the basic, clinical and transformation medical research of diseases.
At present, the cryopreservation of tissues mainly comprises 2 methods, namely a programming method and a vitrification method. The programmed freezing method has the obvious defects of long time consumption, generation of intracellular ice crystals, dependence on instruments and equipment and the like, the vitrification method can effectively avoid the formation of the ice crystals, is simple to operate, has better freezing effect and becomes a hot spot of the freezing research in recent years. Vitrification cryopreservation is the first use of all and Fahy in 1985, and the vitrification solution of mixed multi-element protective agent is adopted by the animals to successfully cryopreserve mouse embryos, and the vitrification cryopreservation method is widely applied to cryopreservation of various biological samples.
Cryopreservation of human oocytes may be performed using either conventional slow freezing or vitrification methods. In conventional slow freezing methods, cells are slowly cooled to very low temperatures, thereby reducing the formation of ice crystals within the cells. At the same time, the low molarity cryoprotectant is used to reduce adverse effects due to increased solute concentrations. Vitrification, on the other hand, is a non-equilibrium cryopreservation method that uses high concentrations of cryoprotectants to coagulate the embryo/oocyte without the formation of ice crystals, since exposure to high concentrations of cryoprotectants helps rapid dehydration prior to placing the embryo/oocyte in liquid nitrogen.
Currently, vitrified frozen carriers are divided into two types, one is an open type frozen carrier, and the other is a closed type frozen carrier. Most animal research laboratories and human assisted reproductive center laboratories use open freezing carrier to accomplish the cryopreservation of gamete and embryo at present because open freezing carrier can make solution direct contact liquid nitrogen, reaches rapid cooling, helps the formation of vitrification state, reduces the ice crystal injury.
However, cross contamination between embryos may exist in the open freezing carrier, or embryo contamination due to liquid nitrogen contamination, and especially for human assisted centers, the use of closed freezing carriers is necessary.
In the prior art, the liquid nitrogen carrier is generally formed by assembling an outer sleeve, an insertion rod, a carrier rod and a slide glass, wherein one end of the outer sleeve is closed, the other end of the outer sleeve is opened to facilitate the insertion and sealing of the carrier rod, and the closed end is provided with metal for balancing the carrier and preventing the carrier from floating in liquid nitrogen. The design has the advantages that: can realize the embryo closed freezing on the carrying rod, and the structural part is not complex, and is easier to process and lower in cost.
The insertion rod and the slide combination in the prior art are inserted into the outer sleeve without a supporting point, so that embryos or cells on the slide are easily directly impacted on metal, and the technical problem of embryo or cell shedding and damage is solved.
Disclosure of Invention
The application provides a closed freezing pole of carrying has solved that prior art's inserted bar and slide glass combination insert not have the strong point in the overcoat pipe, leads to easily that embryo or cell on the slide glass directly strike on the metal, the technical problem who causes embryo or cell to drop and damage that exists.
In view of the above, the present application provides a closed-type freezing carrying bar, comprising:
a carrier rod and a sleeve;
the carrying rod comprises a connecting part, a handheld part and a carrying piece part which are respectively arranged at two ends of the connecting part;
the connecting part comprises a connecting upper part, a connecting middle part and a connecting lower part, and the connecting lower part is connected with the carrying piece part;
the sleeve comprises an outer sleeve and a weight piece;
the outer sleeve is divided into an upper sleeve part, a middle sleeve part and a lower sleeve part, and the counterweight is fixedly arranged in the lower sleeve part;
the cross section size of the upper part of the sleeve is larger than the radial cross section size of the middle part of the sleeve, and the radial cross section size of the middle part of the sleeve is matched with the radial cross section size of the middle part of the connecting part.
Optionally, the radial cross-section of the connecting upper portion is square of a first size;
the radial section of the connecting middle part is a rectangle with a second size;
the radial section of the connecting lower part is circular;
the first size is greater than the second size.
Optionally, the connecting lower portion gradually decreases in size in a radial cross section from one end near the connecting middle portion to the other end.
Optionally, an end of the carrier part far away from the connecting lower part is provided with an ink mark.
Optionally, the radial section of the upper part of the sleeve is a structure with an outer circle and an inner square.
Optionally, the middle portion of the sleeve tapers in size in radial cross-section from a quarter of the way near one end of the upper portion of the sleeve to the other end.
Optionally, the length, the width and the height of the upper connecting portion are respectively: 49 x 2 x 3mm; the length, the width and the height of the middle part of the connection are respectively 1.5 x 2.9 x 3mm; the maximum outer diameter of the connecting lower part is 2.4mm, the minimum outer diameter is 2.0mm, the inner diameter is 1mm, the length of size reduction is 8mm, and the total length is 34mm.
Optionally, the upper portion of the cannula has an inner diameter dimension of 3.1 x 3.1 and a length dimension of 65mm; the inner diameter of the middle part of the sleeve is 3mm, and the length of the middle part of the sleeve is 10mm; the inner diameter of the lower part of the sleeve is 4.4mm, and the length of the lower part of the sleeve is 55mm.
Optionally, the weight member has an outer diameter dimension of 4.3mm and a length dimension of 5mm.
According to the technical scheme, the embodiment of the application has the following advantages:
in this application, a closed freezing pole that carries is provided, includes: a carrier rod and a sleeve; the carrying rod comprises a connecting part, a handheld part and a carrying piece part which are respectively arranged at two ends of the connecting part; the connecting part comprises a connecting upper part, a connecting middle part and a connecting lower part, and the connecting lower part is connected with the carrying piece part; the sleeve comprises an outer sleeve and a counterweight; the outer sleeve is divided into an upper sleeve part, a middle sleeve part and a lower sleeve part, and the counterweight is fixedly arranged in the lower sleeve part; the cross-sectional dimension of the upper part of the sleeve is larger than the radial cross-sectional dimension of the middle part of the sleeve, and the radial cross-sectional dimension of the middle part of the sleeve is matched with the radial cross-sectional dimension of the connecting middle part.
This application is through increasing the sleeve pipe for carrying the pole, realize freezing under the embryo sealed environment, carry out the design of structure and size to carrying pole and sheathed tube upper and lower portion simultaneously, it is spacing to carrying the pole at the sleeve pipe middle part, when avoiding carrying the pole and pack into the sleeve pipe, embryo or the direct striking of cell on the slide glass are on the metal, it does not have the strong point to have solved prior art's inserted bar and slide glass combination insert the overcoat intraductally, lead to embryo or the direct striking of cell on the slide glass on the metal easily, the technical problem that the embryo or cell that cause of existence drop and harm.
Drawings
FIG. 1 is a schematic view of a closed-type freezing bar according to an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a carrier bar in an embodiment of the present application;
FIG. 3 is a schematic structural diagram of a bushing in an embodiment of the present application;
wherein the reference numerals are:
1. a carrier bar; 2. a sleeve; 11. a connecting portion; 12. a hand-held portion; 13. a carrier sheet section; 111. connecting the upper part; 112. connecting the middle part; 113. connecting the lower part; 21. the upper part of the sleeve; 22. the middle part of the sleeve; 23. the lower part of the sleeve; 24. a counterweight member.
Detailed Description
In order to make the technical solutions of the present application better understood, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without making any creative effort belong to the protection scope of the present application.
The application designs a pole is carried in closed freezing, has solved that prior art's inserted bar and slide glass combination insert not have the strong point in the overcoat pipe, leads to embryo or cell on the slide glass directly to strike on the metal easily, the technical problem who causes embryo or cell to drop and damage that exists.
For easy understanding, please refer to fig. 1 to fig. 3, wherein fig. 1 is a schematic structural diagram of a closed-type freezing carrying rod in an embodiment of the present application, as shown in fig. 1, specifically:
a carrier rod 1 and a sleeve 2;
the carrying rod 1 comprises a connecting part 11, a handheld part 12 and a carrying piece part 13 which are respectively arranged at two ends of the connecting part 11;
the connecting part 11 is divided into a connecting upper part 111, a connecting middle part 112 and a connecting lower part 113, and the connecting lower part 113 is connected with the carrying piece part 13;
the sleeve 2 comprises an outer sleeve and a weight 24;
the outer sleeve is divided into a sleeve upper part 21, a sleeve middle part 22 and a sleeve lower part 23, and the counterweight 24 is fixedly arranged in the sleeve lower part 23;
the cross-sectional dimension of the sleeve upper portion 21 is greater than the radial cross-sectional dimension of the sleeve middle portion 22, and the radial cross-sectional dimension of the sleeve middle portion 22 matches the radial cross-sectional dimension of the connecting middle portion 112.
It should be noted that the carrying rod 1 includes a connecting portion 11, a handheld portion 12 and a carrying piece portion 13 respectively disposed at two ends of the connecting portion 11, the handheld portion 12 is used for an operator to operate, the carrying piece portion 13 is used for placing carriers such as cells or embryos, and the connecting portion 11 is used for detachably connecting the carrying rod 1 and the inner sleeve 4.
The radial cross-sectional dimension of the middle portion 22 of the sleeve is matched with the radial cross-sectional dimension of the middle connecting portion 112, so that the carrying rod 1 can be limited, the carrying piece portion 13 of the carrying rod 1 cannot touch the counterweight 24 arranged on the lower portion 23 of the sleeve, and the falling off of cells or embryos on the carrying piece portion 13 is avoided.
Because the cell or embryo of the carrying piece part 13 is lost due to the floating of the carrying rod 1 in the liquid nitrogen, in order to enable the carrying rod 1 to completely sink to the bottom of the liquid nitrogen pail, the minimum weight of the counterweight 24 which can ensure that the carrying rod 1 completely sinks to the bottom of the liquid nitrogen pail can be obtained by calculating the influence factors such as the buoyancy of the carrying rod 1, the volume of the carrying rod 1, the buoyancy of the liquid nitrogen and the weight of the carrying rod 1. Because of the fixed weight, stainless steel material may be selected to increase the density, but reduce the volume of the weight 24.
After the carrying rod 1 is connected with the sleeve 2, sealing and freezing can be carried out without other auxiliary tools.
Further, the radial section of the connecting upper portion 111 is a square of a first size;
the radial cross section of the connecting middle portion 112 is rectangular of a second size;
the radial cross-section of the connecting lower portion 113 is circular;
the first dimension is greater than the second dimension.
Further, the connecting lower portion 113 gradually decreases in size in a radial cross section from one end near the connecting middle portion 112 to the other end.
Further, an end of the carrier plate portion 13 away from the connecting lower portion 113 is provided with an ink mark.
Further, the radial section of the upper sleeve portion 21 is of an outer circle and an inner square structure.
Further, the sleeve middle portion 22 is gradually reduced in size in radial section from a quarter position near one end of the sleeve upper portion 21 to the other end.
Further, the length, width and height of the connecting upper portion 111 are respectively: 49 x 2 x 3mm; the length, width and height of the connecting middle part 112 are respectively 1.5 × 2.9 × 3mm; the maximum outer diameter of the connecting lower portion 113 is 2.4mm, the minimum outer diameter is 2.0mm, the inner diameter is 1mm, the length of the size reduction is 8mm, and the total length is 34mm.
Further, the inner diameter of the upper sleeve portion 21 is 3.1 × 3.1, and the length thereof is 65mm; the inner diameter of the middle part 22 of the sleeve is 3mm, and the length is 10mm; the lower portion 23 of the sleeve has an internal diameter of 4.4mm and a length of 55mm.
Further, the weight member 24 has an outer diameter size of 4.3mm and a length size of 5mm.
In the embodiment of the application, a closed type freezing carrying rod is provided, a sleeve is added for the carrying rod, freezing treatment is carried out under the embryo sealing environment, meanwhile, the structure and the size of the upper, middle and lower parts of the carrying rod and the sleeve are designed, the carrying rod is limited in the middle of the sleeve, when the carrying rod is prevented from being loaded into the sleeve, embryos or cells on a slide sheet directly impact on metal, the problem that in the prior art, no supporting point exists in the outer sleeve in the inserting rod and slide sheet combination inserting process, embryos or cells on the slide sheet directly impact on the metal easily, and the existing technical problems of embryo or cell shedding and damage are caused is solved.
It is clear to those skilled in the art that, for convenience and brevity of description, the specific working processes of the above-described systems, apparatuses and units may refer to the corresponding processes in the foregoing method embodiments, and are not described herein again.
The terms "first," "second," "third," "fourth," and the like (if any) in the description of the present application and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the application described herein are, for example, capable of operation in sequences other than those illustrated or otherwise described herein. Moreover, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
It should be understood that in the present application, "at least one" means one or more, "a plurality" means two or more. "and/or" for describing an association relationship of associated objects, indicating that there may be three relationships, e.g., "a and/or B" may indicate: only A, only B and both A and B are present, wherein A and B may be singular or plural. The character "/" generally indicates that the former and latter associated objects are in an "or" relationship. "at least one of the following" or similar expressions refer to any combination of these items, including any combination of the singular or plural items. For example, at least one (one) of a, b, or c, may represent: a, b, c, "a and b", "a and c", "b and c", or "a and b and c", wherein a, b, c may be single or plural.
The above embodiments are only used to illustrate the technical solutions of the present application, and not to limit the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions in the embodiments of the present application.
Claims (9)
1. A closed freeze bar comprising:
a carrier rod and a sleeve;
the carrying rod comprises a connecting part, a handheld part and a carrying piece part which are respectively arranged at two ends of the connecting part;
the connecting part comprises a connecting upper part, a connecting middle part and a connecting lower part, and the connecting lower part is connected with the carrying piece part;
the sleeve comprises an outer sleeve and a counterweight;
the outer sleeve is divided into an upper sleeve part, a middle sleeve part and a lower sleeve part, and the counterweight is fixedly arranged in the lower sleeve part;
the cross-sectional dimension of the upper part of the sleeve is larger than the radial cross-sectional dimension of the middle part of the sleeve, and the radial cross-sectional dimension of the middle part of the sleeve is matched with the radial cross-sectional dimension of the connecting middle part.
2. A closed-type freeze carrier bar as set forth in claim 1 wherein the radial cross-section of the connecting upper portion is square of a first size;
the radial section of the connecting middle part is a rectangle with a second size;
the radial section of the connecting lower part is circular;
the first size is greater than the second size.
3. A closed-type freeze carrier bar as set forth in claim 2 wherein the connecting lower portion has a decreasing radial cross-sectional dimension from one end proximate the connecting middle portion to the other end.
4. A closed-type freeze carrier bar as set forth in claim 1 wherein an end of the carrier strip portion distal from the connecting lower portion is provided with ink indicia.
5. A closed-type freezing carrying rod according to claim 1, wherein the radial section of the upper part of the sleeve is a structure with an outer circle and an inner square.
6. A closed-type freeze load bar as set forth in claim 1 wherein the central portion of the sleeve tapers in size in radial cross-section from a quarter point near one end of the upper portion of the sleeve to the other end.
7. A closed-type freezing carrying rod according to claim 6, wherein the length, width and height of the connecting upper part are respectively as follows: 49 x 2 x 3mm; the length, the width and the height of the middle part of the connection are respectively 1.5 x 2.9 x 3mm; the maximum outer diameter of the connecting lower part is 2.4mm, the minimum outer diameter is 2.0mm, the inner diameter is 1mm, the length of size reduction is 8mm, and the total length is 34mm.
8. A closed freeze carrier bar according to claim 2 wherein the sleeve upper portion has an inner diameter dimension of 3.1 x 3.1 and a length dimension of 65mm; the inner diameter of the middle part of the sleeve is 3mm, and the length of the middle part of the sleeve is 10mm; the inner diameter of the lower part of the sleeve is 4.4mm, and the length of the lower part of the sleeve is 55mm.
9. A closed-end freeze load bar as set forth in claim 1 wherein the weight member has an outer diameter dimension of 4.3mm and a length dimension of 5mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221877304.2U CN218218932U (en) | 2022-07-19 | 2022-07-19 | Closed freezing pole that carries |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221877304.2U CN218218932U (en) | 2022-07-19 | 2022-07-19 | Closed freezing pole that carries |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218218932U true CN218218932U (en) | 2023-01-06 |
Family
ID=84676039
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221877304.2U Active CN218218932U (en) | 2022-07-19 | 2022-07-19 | Closed freezing pole that carries |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218218932U (en) |
-
2022
- 2022-07-19 CN CN202221877304.2U patent/CN218218932U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kong et al. | Comparison of open pulled straw (OPS) vs glass micropipette (GMP) vitrification in mouse blastocysts | |
| He et al. | Vitrification by ultra-fast cooling at a low concentration of cryoprotectants in a quartz micro-capillary: a study using murine embryonic stem cells | |
| Kuwayama et al. | Highly efficient vitrification method for cryopreservation of human oocytes | |
| Wu et al. | In vitro maturation, fertilization and embryo development after ultrarapid freezing of immature human oocytes | |
| Woods et al. | Fundamental cryobiology of reproductive cells and tissues | |
| Arav et al. | A new, simple, automatic vitrification device: preliminary results with murine and bovine oocytes and embryos | |
| Newton et al. | Osmotically inactive volume, hydraulic conductivity, and permeability to dimethyl sulphoxide of human mature oocytes | |
| JP2017046711A (en) | Improved micromanipulation and storage apparatus and method | |
| JP6475493B2 (en) | Germ cell preservation tool and germ cell preservation method | |
| Withler | Cryopreservation of spermatozoa of some freshwater fishes cultured in South and Southeast Asia | |
| Dobrinsky et al. | Cryopreservation of porcine embryos by vitrification: a study of in vitro development | |
| JP5224703B2 (en) | Collected tissue piece vitrification cryopreservation tool, tissue piece cryopreservation plate, and harvested tissue piece vitrification cryopreservation method | |
| Yamada et al. | Immature bovine oocyte cryopreservation: comparison of different associations with ethylene glycol, glycerol and dimethylsulfoxide | |
| EP3522076A1 (en) | Individual management system | |
| Carmichael et al. | Cryopreservation and in vitro fertilization at the zebrafish international resource center | |
| Gupta et al. | Effect of cryopreservation technique and season on the survival of in vitro produced cattle embryos | |
| Manjunatha et al. | In vitro embryo development and blastocyst hatching rates following vitrification of river buffalo embryos produced from oocytes recovered from slaughterhouse ovaries or live animals by ovum pick-up | |
| Nowshari et al. | Effect of freezing rate and exposure time to cryoprotectant on the development of mouse pronuclear stage embryos | |
| CN218218932U (en) | Closed freezing pole that carries | |
| Saragusty | Directional freezing for large volume cryopreservation | |
| Tsang et al. | Cryopreservation of mammalian embryos: Advancement of putting life on hold | |
| Cho et al. | Improvement in post-thaw viability of in vitro-produced bovine blastocysts vitrified by glass micropipette (GMP) | |
| Lee et al. | An efficient and mass reproducible method for vitrifying mouse embryos on a paper in cryotubes | |
| JP2007261973A (en) | Vitrifying preservation instrument for embryo, and method for vitrifying preservation of embryo | |
| Fuller et al. | Cryopreservation of mammalian embryos |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |