CN218202737U - Card box - Google Patents

Abstract

An embodiment of the utility model provides a card box, card box includes: the kit comprises a cartridge body, a detection device and a control device, wherein the cartridge body comprises a central hole groove and a plurality of reagent storage structures, the reagent storage structures are used for providing space for samples or reagents for sample detection, liquid discharge holes are formed in the side walls, facing the central hole groove, of the reagent storage structures, and the liquid discharge holes of the plurality of reagent storage structures are located at the same height; the transfer device is positioned in the central hole groove and can rotate in the central hole groove, a liquid transfer hole is formed in the side wall of the transfer device, the liquid transfer hole and the liquid discharge hole are positioned at the same height, and when the transfer device rotates and moves to a designated position, the liquid transfer hole is communicated with the liquid discharge hole of a reagent storage structure. The embodiment of the utility model provides a can reduce the sample or be used for the reagent of sample detection to receive the probability of pollution in testing process.

Description

Card box

Technical Field

The embodiment of the utility model provides a relate to gene sequencing technical field, especially relate to a card box.

Background

In many chemical or biological assays and tests, it is often necessary to perform multiple processing steps on a sample. For example, in the case of nucleic acid detection, it is necessary to perform multiple steps of processing such as lysis, binding, washing, elution, and amplification on an obtained sample. In the related art, a manual experiment method is generally adopted, and an experimenter completes nucleic acid extraction by using a nucleic acid extractor, and then completes Quantitative analysis by using a QPCR (Real-time Quantitative PCR detection System) instrument. However, in the current method of manual experiment, it is necessary to transfer the sample or reagent many times manually, but the sample or reagent is very easy to be polluted during the transfer process.

For this reason, it is desirable to provide a cartridge that reduces the probability of contamination of the sample or reagents used in the detection of the sample during the detection process.

SUMMERY OF THE UTILITY MODEL

In view of this, the embodiments of the present invention provide a cartridge, which can reduce the probability that a sample or a reagent for sample detection is contaminated during the detection process.

In a first aspect, an embodiment of the present invention provides a cartridge, including:

the kit comprises a cartridge body, a detection device and a control device, wherein the cartridge body comprises a central hole groove and a plurality of reagent storage structures, the reagent storage structures are used for providing space for samples or reagents for sample detection, liquid discharge holes are formed in the side walls, facing the central hole groove, of the reagent storage structures, and the liquid discharge holes of the plurality of reagent storage structures are located at the same height;

the transfer device is positioned in the central hole groove and can rotate in the central hole groove, a liquid transfer hole is formed in the side wall of the transfer device, the liquid transfer hole and the liquid discharge hole are positioned at the same height, and when the transfer device rotates and moves to a designated position, the liquid transfer hole is communicated with the liquid discharge hole of a reagent storage structure.

Optionally, the reagent storage structure is further provided with an exhaust hole facing the side wall of the central hole groove, and the exhaust holes of the plurality of reagent storage structures are located at the same height; the transfer device is also provided with a gas transfer hole; when the transfer device rotates and moves to a designated position, the air transfer hole is communicated with an exhaust hole of a reagent storage structure.

Optionally, the plurality of reagent storage structures comprises: a nucleic acid extraction well, a PCR reaction well and a plurality of reagent storage wells.

Optionally, the plurality of reagent storage holes are used for containing different reagents, and the plurality of reagent storage holes include a first reagent storage hole, a second reagent storage hole, a third reagent storage hole, a fourth reagent storage hole, and a fifth reagent storage hole, where the first reagent storage hole is used for providing a containing space for a sample, the second reagent storage hole is used for storing lysis solution, the third reagent storage hole is used for storing washing solution, the fourth reagent storage hole is used for storing elution solution, and the fifth reagent storage hole is used for storing PCR reaction solution; the nucleic acid extraction hole is used for providing a reaction space for nucleic acid extraction, and the PCR reaction hole is used for providing a reaction space for PCR reaction liquid.

Optionally, the nucleic acid extraction hole and the PCR reaction hole are disposed at two sides of the central hole groove, and the plurality of reagent storage holes are distributed oppositely by taking the nucleic acid extraction hole and the PCR reaction hole as a symmetry line.

Optionally, the transfer device is a syringe, the syringe includes a piston and a syringe barrel, the piston is inserted in the syringe barrel, and the piston is used for reciprocating movement in the syringe barrel to transfer the sample or a reagent for sample detection.

Optionally, the bottom of the syringe barrel is provided with a keyway keyed to a rotating motor of the cartridge body.

Optionally, the syringe further comprises a rubber layer, and the rubber layer is arranged on the outer wall of the syringe barrel.

Optionally, the nucleic acid extraction well comprises a nucleic acid well main body, and a first pipeline and a second pipeline connected to the nucleic acid well main body, wherein the first pipeline is connected to the liquid discharge hole of the nucleic acid extraction well, and the second pipeline is connected to the gas discharge hole of the nucleic acid extraction well; the nucleic acid well body protrudes from the plurality of reagent storage wells; the PCR reaction hole comprises a PCR reaction hole main body, a third pipeline and a fourth pipeline, wherein the third pipeline and the fourth pipeline are connected with the PCR reaction hole main body; the PCR reaction hole main body protrudes out of the plurality of reagent storage holes.

Optionally, the plurality of reagent storage holes include a first second reagent combination hole, a third reagent storage hole, a fourth reagent storage hole, and a fifth reagent storage hole, the first second reagent combination hole is used for accommodating a sample and a lysis solution at the same time, the third reagent storage hole is used for storing a washing solution, the fourth reagent storage hole is used for storing an eluent, and the fifth reagent storage hole is used for storing a PCR reaction solution; the nucleic acid extraction hole is used for providing a reaction space for nucleic acid extraction, and the PCR reaction hole is used for providing a reaction space for PCR reaction liquid.

Optionally, a plurality of reagent storage holes include first reagent storage hole, third reagent storage hole, fourth reagent storage hole and fifth reagent storage hole, wherein, the nucleic acid extraction hole is used for providing reaction space for lysate and nucleic acid extraction, the PCR reaction hole is used for providing reaction space for the PCR reaction liquid, and first reagent storage hole is used for providing accommodation space for the sample, and third reagent storage hole is used for storing the washing liquid, fourth reagent storage hole is used for storing the eluant, fifth reagent storage hole is used for storing the PCR reaction liquid.

Optionally, the PCR reaction hole is packaged with PCR reaction liquid, the nucleic acid extraction hole is used for providing reaction space for nucleic acid extraction, a plurality of reagent storage holes include first reagent storage hole, second reagent storage hole, third reagent storage hole, fourth reagent storage hole, and first reagent storage hole is used for providing accommodation space for the sample, second reagent storage hole is used for storing the lysate, and third reagent storage hole is used for storing the washing liquid, fourth reagent storage hole is used for storing the eluant.

Optionally, the cartridge further comprises a magnet, magnetic beads are arranged in the nucleic acid extraction hole, and the magnet is fixed by a clamping mechanism and arranged below the nucleic acid extraction hole; the clamping mechanism is movable, and the magnet is based on the movement of the clamping mechanism, and adsorbs magnetic beads in the nucleic acid extraction hole when being close to the nucleic acid extraction hole.

Optionally, the cartridge further comprises a fluorescent quantitative detection device, the fluorescent quantitative detection device is used for monitoring the nucleic acid concentration of the reaction solution in the PCR reaction hole, and the included angle between the light source end of the fluorescent quantitative detection device and the light source of the detection end is 60-120 °.

Optionally, the height of the exhaust hole is higher than that of the liquid discharge hole; the height of the gas transfer hole is higher than that of the liquid transfer hole.

The embodiment of the utility model discloses card box, include: the cartridge comprises a cartridge body and a plurality of reagent storage structures, wherein the cartridge body comprises a central hole groove and the reagent storage structures are used for providing space for a sample or a reagent for sample detection, the side walls of the reagent storage structures facing the central hole groove are provided with liquid discharge holes, and the liquid discharge holes of the reagent storage structures are positioned at the same height; the transfer device is positioned in the central hole groove and can rotate in the central hole groove, the side wall of the transfer device is provided with a liquid transfer hole, the liquid transfer hole and the liquid discharge hole are positioned at the same height, and when the transfer device rotates and moves to a designated position, the liquid transfer hole is communicated with the liquid discharge hole of a reagent storage structure. The embodiment of the utility model provides a behind transfer device rotary motion to assigned position, realize that the weeping hole on the transfer device lateral wall is linked together with the outage of a reagent storage structure, and then can realize the liquid transfer between transfer device and a reagent storage structure, need not to expose the sample in the testing process or sample detection's reagent in the air, and avoided artifical the participation probably pollution that causes, for this reason, the utility model provides a card box can be at the inside sample or the reagent that is used for sample detection of shifting of the card box of same card box, has reduced the sample or has been used for sample detection's reagent to receive the probability of pollution in the testing process, can promote the detection efficiency of sample.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

Fig. 1 is a schematic view of a cartridge provided in an embodiment of the present invention;

FIG. 2 is a schematic view of a cartridge body according to an embodiment of the present invention;

FIG. 3 is a schematic view of another angle of the main body of the cartridge in the embodiment of the present invention;

fig. 4 is an alternative schematic view of a transfer device provided in an embodiment of the present invention;

fig. 5 is a flowchart of a detection method according to an embodiment of the present invention;

fig. 6 is a schematic flow chart of another detection method according to an embodiment of the present invention;

FIG. 7 is a schematic view of an embodiment of the present invention showing a state where an injector communicates with a first reagent storage well of a cartridge body;

FIG. 8 is a schematic view showing a state where the injector is not connected to the nucleic acid extracting hole of the cartridge body in the embodiment of the present invention;

FIG. 9 is a schematic view showing a state where an injector communicates with a nucleic acid extracting hole of a cartridge main body according to an embodiment of the present invention;

FIG. 10 is a schematic view showing the communication state between the syringe and the PCR reaction well of the cartridge body according to the embodiment of the present invention.

Detailed Description

As known from the background art, in the prior art, in the process of detecting a sample, the sample or a reagent needs to be transferred manually for many times, and the sample or the reagent is easily polluted in the process of transferring the sample or the reagent. Utility model people discovers, this is because, at the artifical in-process that realizes sample or reagent and shift, need utilize the pipettor as the transfer device, extract sample or reagent many times to after the extraction, manually move to the container that corresponds in, this process can inevitably take the pipettor, make the injection port of pipettor extraction liquid very easily contact the air, and then probably be polluted by the pollutant in the air.

In view of this, embodiments of the present invention provide a cartridge, which includes: the kit comprises a cartridge body, a detection device and a control device, wherein the cartridge body comprises a central hole groove and a plurality of reagent storage structures, the reagent storage structures are used for providing space for samples or reagents for sample detection, liquid discharge holes are formed in the side walls, facing the central hole groove, of the reagent storage structures, and the liquid discharge holes of the plurality of reagent storage structures are located at the same height; the transfer device is positioned in the central hole groove and can rotate in the central hole groove, the side wall of the transfer device is provided with a liquid transfer hole, the liquid transfer hole and the liquid discharge hole are positioned at the same height, and when the transfer device rotates and moves to a designated position, the liquid transfer hole is communicated with the liquid discharge hole of a reagent storage structure.

It can be seen that in the embodiment of the utility model, sample and reagent can set up in the different reagent storage structures of same card box, and the swivelling movement through transfer device, the liquid discharge hole that can realize transfer device moves liquid hole and different reagent storage structures is linked together to realize the liquid-transfering of sample and reagent and handle, and should move liquid the process leakproofness better, avoided artifical the pollution that participates in and probably causes, and need not expose in the air, thereby reduced sample or reagent and received the probability of pollution, and then promoted the detection efficiency of sample.

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Fig. 1 is a schematic view of a cartridge provided in an embodiment of the present invention. Specifically, referring to fig. 1, the cartridge comprises:

a cartridge body 10, wherein the cartridge body 10 includes a central hole groove 110 and a plurality of reagent storage structures 100, the reagent storage structures 100 are used for providing a space for a sample or a reagent for sample detection, the reagent storage structures are provided with liquid discharge holes (not shown) towards the side wall of the central hole groove, and the liquid discharge holes of the plurality of reagent storage structures are located at the same height;

the cartridge body 10 is used to carry the transfer device 20 and to provide space for samples or reagents for sample testing. In one embodiment, the reagent storage structure 100 of the cartridge body 10 may include a nucleic acid extraction well 120, a PCR reaction well 130, which may provide a reaction space for nucleic acid extraction and a PCR reaction well, which may provide a reaction space for PCR reaction solution, and a plurality of reagent storage wells, which may be used to accommodate different reagents.

The transfer device 20 is positioned in the central hole groove, the transfer device 20 can rotate in the central hole groove 110, a liquid transfer hole is formed in the side wall of the transfer device, the liquid transfer hole and the liquid discharge hole are positioned at the same height, and when the transfer device rotates and moves to a designated position, the liquid transfer hole is communicated with the liquid discharge hole of a reagent storage structure.

The utility model provides a transfer device 20 can be the syringe, and when the liquid transfer hole that the lateral wall of syringe was seted up and the outage intercommunication of a reagent storage structure, can realize the liquid transfer between transfer device and this reagent storage structure.

Alternatively, the arrows in FIG. 1 indicate the clockwise rotation of the transfer device 20 within the central bore 110. In other alternative embodiments, the transfer device 20 may also be rotated counterclockwise within the central bore, which is not illustrated here.

Based on above, the embodiment of the utility model provides a after transfer device rotary motion to assigned position, realized that the drain hole that moves liquid hole and a reagent storage structure on the transfer device lateral wall are linked together, after the intercommunication, can realize the liquid transfer between transfer device and the reagent storage structure, need not to expose the sample among the testing process or sample detection's reagent in the air, and the pollution that probably causes when having avoided artifical the participation, for this reason, the utility model provides a card box can shift the sample or be used for sample detection's reagent in the inside transfer sample of card box of same card box, has reduced sample or reagent and has received the probability of pollution in the testing process, can promote the detection efficiency of sample.

Based on the above, the respective components in the cartridge main body in the embodiment of the present invention will be described below. Fig. 2-3 are schematic views of the cartridge body in the embodiment of the present invention. Fig. 2 is a schematic view of a reference of the cartridge body according to an embodiment of the present invention, and fig. 3 is a schematic view of another angle of the cartridge body according to an embodiment of the present invention.

Referring to FIGS. 2 and 3, the cartridge body 10 includes a central hole groove 110 and a plurality of reagent storage structures including a nucleic acid extracting well 120, a PCR reaction well 130, and a plurality of reagent storage wells. The side wall of any one reagent storage structure facing the central hole groove is provided with liquid discharge holes (such as liquid discharge holes 1022b and 1022c in fig. 2), the liquid discharge holes (such as liquid discharge holes 1022b and 1022c in fig. 2) of a plurality of reagent storage structures are positioned at the same height, and the liquid discharge holes of the reagent storage structures are aligned with the liquid transfer holes of the transfer device when the transfer device rotates, so that the samples or the liquid of the reagents for sample detection between the reagent storage structures and the transfer device can be transferred.

The utility model discloses in other optional realizations of the embodiment, at the lateral wall of reagent storage structure, can also be provided with the exhaust hole that is higher than the outage. Specifically, the reagent storage structure is provided with vent holes (such as vent holes 1021b and vent holes 1021c in fig. 2) facing the side wall of the central hole groove, the vent holes of the plurality of reagent storage structures are located at the same height, and when the vent holes of the plurality of reagent storage structures are communicated with the air moving holes on the transfer device, the balance of air pressure between the reagent storage structures and the transfer device can be maintained, and meanwhile, the liquid transfer between the reagent storage structures and the transfer device is realized.

The embodiment of the utility model provides a transfer device is rotatory when moving target location, can realize the sample between reagent storage structure and the transfer device or the transfer of the liquid of sample testing's reagent. Further, the embodiment of the utility model provides an on the basis of seting up the outage on reagent storage structure, the exhaust hole has been seted up simultaneously, when reagent storage structure's outage aligns with transfer device's transfer liquid hole, reagent storage structure's exhaust hole also aligns with the last gas pocket that moves of transfer device, and at this moment, when can realize maintaining the atmospheric pressure balance between the cavity that reagent storage structure and transfer device formed, the transfer of liquid between realization reagent storage structure and the transfer device.

Optionally, the nucleic acid extraction hole and the PCR reaction hole are disposed at two sides of the central hole groove, and the plurality of reagent storage holes are distributed oppositely by taking the nucleic acid extraction hole and the PCR reaction hole as a symmetry line. In an alternative embodiment, the nucleic acid extracting well is positioned on the same axis as the PCR reaction well, the central well groove and the PCR reaction well, and the plurality of reagent storage wells are oppositely disposed with the nucleic acid extracting well, the central well groove and the PCR reaction well as a line of symmetry, so that the cartridge body is balanced.

Referring to FIG. 3, in one embodiment, the nucleic acid extraction well 120 and the PCR reaction well 130 are each a single layer structure and are fixed to the cartridge body by joining.

In addition, referring to fig. 1, the reagent storage structure 100 comprises a nucleic acid extraction well 120 and a PCR reaction well 130, wherein the nucleic acid extraction well and the PCR reaction well are positioned at two sides of the cartridge body, specifically, the nucleic acid extraction well 120 comprises a nucleic acid well body 125, and a first pipeline 126 and a second pipeline 127 connected to the nucleic acid well body 125, the first pipeline 126 is connected to a liquid discharge hole of the nucleic acid extraction well, and the second pipeline 127 is connected to a gas discharge hole of the nucleic acid extraction well; the nucleic acid well body 125 protrudes from the plurality of reagent storage wells; the PCR reaction well 130 includes a PCR reaction well main body 135, and a third pipe 136 and a fourth pipe 137 connected to the PCR reaction well main body 135, the third pipe being connected to the liquid discharge hole of the PCR reaction well, the fourth pipe being connected to the gas discharge hole of the PCR reaction well; the PCR reaction well main body protrudes from the plurality of reagent storage wells.

The nucleic acid extraction hole and the PCR reaction hole in the embodiment of the utility model are both flat. With the flat structure as shown in FIG. 3, the contact area between the reagents contained in the nucleic acid isolation well or the PCR reaction well and the heat source can be increased, and the elevation rate of the reaction solution contained in the well can be effectively increased.

Furthermore, the flat one side or multiple sides of the nucleic acid extraction hole and the PCR reaction hole are packaged by films, so that when the nucleic acid extraction hole and the PCR reaction hole need to be heated, an external heat source can quickly raise and lower the temperature of reaction liquid in the nucleic acid extraction hole and the PCR reaction hole so as to meet the requirement of the reaction liquid on the temperature.

Optionally, the external heat source of the nucleic acid extraction hole may be at least one of a ceramic heating plate, a PI membrane and a semiconductor cooling plate; the external heat source of the PCR reaction well may be a semiconductor cooling plate, and the external heat source in the embodiment of the present invention may be other heat sources, which are not listed here.

Additionally, the utility model discloses well nucleic acid extraction hole and PCR reaction hole's flat one side or multilateral adopt the film encapsulation, can make things convenient for the transportation and the storage of card box, prevent that the reagent of storage from taking place reagent in a plurality of reagent storage structures and revealing or reagent is contaminated in the transportation.

In addition to the above-described nucleic acid extraction wells and PCR reaction wells, as shown in fig. 2, the plurality of reagent storage structures may further include: the plurality of reagent storage wells are specifically a first reagent storage well 101, a second reagent storage well 102, a third reagent storage well 103, a fourth reagent storage well 104 and a fifth reagent storage well 105, and the plurality of reagent storage wells (the first reagent storage well 101, the second reagent storage well 102, the third reagent storage well 103, the fourth reagent storage well 104 and the fifth reagent storage well 105) are arranged in opposition to each other with the nucleic acid extraction well and the PCR reaction well as a line of symmetry. And the fifth reagent storage hole is the reagent storage hole closest to the PCR reaction hole, and the fourth reagent storage hole is the reagent storage hole closest to the nucleic acid extraction hole, so that the pipetting distance is shortest, and mutual contamination among liquids is reduced.

The embodiment of the utility model provides an in another optional realization, a plurality of reagent store holes include first two reagent merging holes, third reagent store hole, fourth reagent store hole and fifth reagent store hole, and first two reagent merging holes are used for holding sample and lysate simultaneously, and third reagent store hole is used for saving the washing liquid, fourth reagent store hole is used for saving the eluant, fifth reagent store hole is used for saving PCR reaction liquid.

Compared with the previous embodiment, the first and second reagent merging holes in the embodiment of the present invention can establish a larger reagent merging hole based on the position of the first reagent storing hole and the second reagent storing hole, and the first and second reagent merging holes can simultaneously accommodate the sample and the lysate, and the first and second reagent merging holes in the embodiment can be used as an alternative mode of the first reagent storing hole and the second reagent storing hole.

In yet another optional implementation of the embodiments of the present invention, the plurality of reagent storage holes include a first reagent storage hole, a third reagent storage hole, a fourth reagent storage hole and a fifth reagent storage hole, and the plurality of reagent storage structures include a nucleic acid extraction hole, a PCR reaction hole, the first reagent storage hole, the third reagent storage hole, the fourth reagent storage hole and the fifth reagent storage hole. The nucleic acid extraction hole is used for providing a reaction space for lysis solution and nucleic acid extraction, the PCR reaction hole is used for providing a reaction space for the PCR reaction solution, the first reagent storage hole is used for providing an accommodation space for a sample, the third reagent storage hole is used for storing washing liquid, the fourth reagent storage hole is used for storing eluent, and the fifth reagent storage hole is used for storing the PCR reaction solution. Only four reagent storage wells are required to achieve storage of the sample or reagent.

The embodiment of the utility model provides an in another optional realization, the PCR reaction hole is packaged with PCR reaction liquid, the nucleic acid extraction hole is used for providing reaction space for nucleic acid extraction, a plurality of reagent storage holes include first reagent storage hole, second reagent storage hole, third reagent storage hole, fourth reagent storage hole, and first reagent storage hole is used for providing accommodation space for the sample, second reagent storage hole is used for storing the lysate, and third reagent storage hole is used for storing the washing liquid, fourth reagent storage hole is used for storing the eluant, and is visible, also only needs four reagent storage holes to realize the storage of sample or reagent this moment.

In order to realize the detection of the concentration of the reaction liquid, the card box also comprises a fluorescent quantitative detection device, the fluorescent quantitative detection device is used for monitoring the concentration of the nucleic acid in the reaction liquid in the PCR reaction hole, and the included angle between the light source end of the fluorescent quantitative detection device and the light source of the detection end is 60-120 degrees.

In an alternative embodiment, the light source end of the fluorescence quantitative detection device and the light source of the detection end form an included angle of 90 degrees, and the accuracy of nucleic acid concentration monitoring is highest at the included angle. Of course, there is an error in actual use, and it is specifically based on an ideal light source included angle that can be provided between the light source end of the fluorescence quantitative detection device and the detection end, and details are not described here.

Further, the card box also comprises a magnet (not shown in the figure), magnetic beads are arranged in the nucleic acid extraction holes, and the magnet is fixed by a clamping mechanism and is arranged below the nucleic acid extraction holes; the clamping structure is movable, and the magnet is based on the movement of the clamping mechanism and adsorbs magnetic beads in the nucleic acid extraction holes when being close to the nucleic acid extraction holes.

In an optional realization, the nucleic acid is stored in the nucleic acid extraction hole, and the magnetic bead is arranged in the nucleic acid extraction hole, and the magnetic bead can adsorb nucleic acid, and then when the magnet is close to the nucleic acid extraction hole, the magnetic bead of the nucleic acid extraction hole is adsorbed by the magnet, and nucleic acid is adsorbed on the magnetic bead, and the syringe can absorb liquid except for nucleic acid and transfer the liquid as waste liquid.

The embodiment of the utility model provides an in still provide the transfer device who is located the centre bore groove. Fig. 4 is an alternative schematic diagram of a transfer device provided in an embodiment of the present invention.

As shown in fig. 4, the transferring device in the embodiment of the present invention is a syringe, the syringe is rotatable in the central hole groove, the sidewall of the syringe is provided with a liquid transferring hole 222, the liquid transferring hole 222 and the liquid discharging hole of the reagent storage structure are located at the same height, and when the syringe is rotated to a designated position, the liquid transferring hole is communicated with the liquid discharging hole of the reagent storage structure.

In a further embodiment, the injector may also be provided with a gas transfer port 221 corresponding to the reagent storage structure of the cartridge body. When the injector rotates to a designated position, the air vent 221 of the injector can be communicated with an air vent of a reagent storage structure, and when the air vent of the injector is communicated with the air vent of the reagent storage structure, the balance of air pressure between the reagent storage structure and the transfer device can be maintained.

In an alternative embodiment, the shape of the opening of the air vent 221 of the injector may be square (as shown in fig. 4), but in the embodiment of the present invention, the shape of the opening of the air vent 221 may also be round, which is not described herein again.

The shape of the opening of the pipetting hole 222 of the syringe can refer to the shape of the opening of the pipetting hole 221 of the syringe described above and will not be described herein.

In addition, the height of the gas transfer hole 221 of the syringe is not lower than the height of the liquid transfer hole 222. When the utility model discloses when moving the exhaust hole that moves the gas pocket and reagent storage structure in the syringe and the liquid discharge hole that moves the liquid pocket and reagent storage structure in the syringe is linked together simultaneously, can maintain the equilibrium of atmospheric pressure between reagent storage structure and the transfer device, realize the transfer of liquid between reagent storage structure and the transfer device.

In an alternative embodiment, the syringe comprises a piston 200 and a syringe barrel 210, wherein the piston 200 is inserted in the syringe barrel 210, and the piston is inserted in the syringe barrel for forming a cavity between the piston and the syringe barrel to contain liquid when the piston moves back and forth.

In addition, the piston in the embodiment of the present invention can reciprocate in the syringe barrel to realize the transfer of the sample or the reagent for sample detection under the premise that the external drive provides power.

In one embodiment, the external drive may be a screw mechanism, a crank block, a belt transmission, or other drive structure, and the piston may reciprocate in the syringe barrel under the power of the drive structure, so as to transfer the sample or the reagent for sample detection.

Further, the injector of the embodiment of the present invention may further include: a key slot 230 provided at the bottom of the syringe barrel for keying to a rotary motor (not shown) of the cartridge body to effect rotation in a central bore slot in the cartridge body upon actuation of the rotary motor.

In addition, the injector also comprises a rubber layer which is arranged on the outer wall of the injector cylinder, so that the seal between the injector and the central hole groove can be realized, and the seal between the injector and the central hole groove is ensured.

Based on above, the embodiment of the utility model provides a still provide the detection method who corresponds with the card box to the realization is to the detection of sample. Fig. 5 is a flowchart of a detection method according to an embodiment of the present invention. Specifically, referring to fig. 5, the detection method includes:

s51, obtaining a card box main body provided with a transfer device, wherein the card box main body comprises a central hole groove and a plurality of reagent storage structures, the reagent storage structures are used for providing space for a sample or a reagent for sample detection, liquid drainage holes are formed in the side walls of the reagent storage structures, which face the central hole groove, and the liquid drainage holes of the plurality of reagent storage structures are located at the same height;

s52, rotating and moving a transfer device of the central hole groove to a designated position, and transferring a sample or a reagent for sample detection between the transfer device and a reagent storage structure, wherein a liquid transfer hole is formed in the side wall of the transfer device, and the liquid transfer hole and the liquid discharge hole are located at the same height; and under the designated position, the liquid transfer hole of the transfer device is communicated with a liquid discharge hole of a reagent storage structure.

It can be seen that the embodiment of the utility model provides a card box can shift the sample or be used for sample detection's reagent in the card box of same card box is inside, has reduced the probability that receives the pollution in the testing process, can promote the detection efficiency of sample.

It should be noted that the reagent storage structure is further provided with an exhaust hole facing the side wall of the central hole groove, and the exhaust holes of the plurality of reagent storage structures are located at the same height; the transfer device is also provided with a gas transfer hole, and the exhaust hole is arranged corresponding to the gas transfer hole;

in the step of transferring the sample or the reagent for sample detection between the transfer device and the reagent storage structure, when the transfer device located in the central hole groove is rotated and moved to a designated position, the air moving hole on the transfer device is communicated with the air exhaust hole of the reagent storage structure.

Optionally, the embodiment of the present invention provides a cartridge main body as shown in fig. 2 or fig. 3, the cartridge main body includes a central hole groove and a plurality of reagent storage structures, the plurality of reagent storage structures of the cartridge main body include a nucleic acid extraction hole, a PCR reaction hole and a plurality of reagent storage holes, the plurality of reagent storage holes may include a first reagent storage hole, a second reagent storage hole, a third reagent storage hole, a fourth reagent storage hole and a fifth reagent storage hole, in this embodiment, the first reagent storage hole is used for providing a containing space for a sample, the second reagent storage hole is used for storing a lysate, the third reagent storage hole is used for storing a washing solution, the fourth reagent storage hole is used for storing an eluent, and the fifth reagent storage hole is used for storing a PCR reaction solution.

The transfer device can be a syringe, the syringe is shown in fig. 4, the syringe comprises a piston 200 and a syringe barrel 210, the piston 200 is inserted in the syringe barrel 210, and the piston 200 is used for reciprocating in the syringe barrel to realize the transfer of the sample or the reagent for sample detection; the injector may also be provided with a keyway keyed to a rotary motor of the cartridge body to enable rotation within a central bore slot.

Based on the above, in order to implement the detection method in the embodiment of the present invention, fig. 6 is a schematic flow chart of another detection method in the embodiment of the present invention. Specifically, as shown in fig. 6, after the cartridge body with the syringe mounted therein is obtained, the steps of rotating the transfer device for moving the central hole slot to a predetermined position and transferring the sample or the reagent for sample detection between the transfer device and the reagent storage structure include:

s61, rotating an injector to a first reagent storage hole of the cartridge body, wherein the injector sucks a sample contained in the first reagent storage hole based on a first channel, the first channel comprises a first liquid discharge channel and a first gas discharge channel, the first liquid discharge channel is formed when a liquid transfer hole of the injector is communicated with the liquid discharge hole of the first reagent storage hole, and the first gas discharge channel is formed when a gas transfer hole of the injector is communicated with the gas discharge hole of the first reagent storage hole;

optionally, the first reagent storage well is used for providing a containing space for the sample, and after the sample is added into the first reagent storage well, the injector is in an initialization state, and pipetting is started based on the initialization state to complete detection of the sample.

FIG. 7 is a schematic view showing a state in which an injector communicates with a first reagent storage well of a cartridge body according to an embodiment of the present invention. Referring to FIG. 7, when the injector is rotated to the first reagent storage well of the cartridge body, the gas transfer hole 221 of the injector is aligned with the gas discharge hole 1021a of the first reagent storage well of the cartridge body, and the liquid transfer hole 222 of the injector is aligned with the liquid discharge hole 1022a of the first reagent storage well of the cartridge body, so that the sucking of the sample temporarily contained in the first reagent storage well by the injector can be achieved. In addition, since the air vent hole communicates with the air transfer hole and the liquid discharge hole communicates with the liquid transfer hole, for this reason, the air pressure between the syringe and the cartridge body is also balanced during the liquid transfer between the syringe and the cartridge body.

In addition, since the plurality of reagent storage wells have similar structures, the state in which the syringe communicates with any one of the reagent storage wells of the cartridge body can be referred to in FIG. 7, and will not be described again.

S62, rotating the injector to a second reagent storage hole of the card box main body, and sucking the lysate in the second reagent storage hole by the injector through a second channel, wherein the second channel comprises a second liquid drainage channel and a second exhaust channel, the second liquid drainage channel is formed when the liquid drainage hole of the injector is communicated with the liquid drainage hole of the second reagent storage hole, and the second exhaust channel is formed when the gas drainage hole of the injector is communicated with the exhaust hole of the second reagent storage hole;

in a further embodiment of the present invention, the first reagent storage well and the second reagent storage well may be combined to form a first and second reagent combining well, and the first and second reagent combining wells may be formed by a predetermined splitting process of the sample. In this embodiment, the first and second reagent wells may be added to the sample and the lysate simultaneously or sequentially, and after the lysis treatment of the sample by the lysate, the syringe may aspirate the mixture of the lysate and the sample to perform the subsequent treatment.

In addition, in other embodiments of the present invention, it may be preset to add the lysis buffer stored in the second reagent storage well to the nucleic acid extraction well, and in this case, the second reagent storage well is omitted and the remaining reagent storage wells remain unchanged, in which case, step S63 may be directly performed after step S61, and the detection process may also be implemented. In addition, when the volume of the cartridge body is small, the above-mentioned mode of omitting one reagent storage hole may be adopted, and the embodiment of the present invention preferably uses the mode of coexisting five reagent storage holes as described above, so that when the sample or reagent in any one of the reagent storage holes is not enough, the corresponding sample or reagent can be added in time.

And S63, rotating the injector to the nucleic acid extraction hole of the cartridge body, and transferring the liquid in the injector to the nucleic acid extraction hole through a nucleic acid channel, wherein the nucleic acid channel is formed when the liquid transfer hole of the injector is communicated with the liquid discharge hole of the nucleic acid extraction hole, the nucleic acid channel comprises a nucleic acid liquid discharge channel and a nucleic acid exhaust channel, the nucleic acid liquid discharge channel is formed when the liquid transfer hole of the injector is communicated with the liquid discharge hole of the nucleic acid reagent storage hole, and the nucleic acid exhaust channel is formed when the liquid transfer hole of the injector is communicated with the exhaust hole of the nucleic acid reagent storage hole.

Optionally, before the injector is rotated to reach the position of the nucleic acid extraction hole, the pipetting hole on the injector is not aligned with the liquid discharge hole on the cartridge body, and the gas transfer hole on the injector is not aligned with the exhaust hole on the cartridge body, and at this time, the injector is not communicated with the nucleic acid extraction hole of the cartridge body; when the rotary injector rotates to a designated position, the rotary injector is aligned with the nucleic acid extraction hole, the liquid transfer hole on the injector is aligned with the liquid discharge hole on the card box body, and the gas transfer hole on the injector is aligned with the exhaust hole on the card box body.

Referring specifically to FIGS. 8 to 9, FIG. 8 is a schematic view showing a state in which the injector is not connected to the nucleic acid extracting well of the cartridge body according to the embodiment of the present invention; FIG. 9 is a schematic view showing a state where an injector communicates with a nucleic acid extracting hole of a cartridge main body according to an embodiment of the present invention.

As shown in FIG. 8, when the syringe 20 is in a state of non-communication with the nucleic acid extracting hole of the cartridge body 10, the reagent is stored in the syringe, the gas transfer hole of the syringe is in a state of non-communication with the gas discharge hole 121f of the nucleic acid extracting hole, and the liquid transfer hole of the syringe is in a state of non-communication with the liquid discharge hole 122f of the nucleic acid extracting hole; when the syringe 20 is rotated and the syringe is in communication with the nucleic acid extraction hole of the cartridge body 10, as shown in FIG. 9, the gas transfer hole 221 of the syringe 20 is aligned with the gas discharge hole 121f of the nucleic acid extraction hole to form a nucleic acid gas discharge passage, and the liquid transfer hole 222 of the syringe 20 is aligned with the liquid discharge hole 122f of the nucleic acid extraction hole to form a nucleic acid liquid discharge passage.

Optionally, when the cracking operation is completed in the nucleic acid extraction hole, the magnet fixed by the clamping mechanism is close to the nucleic acid extraction hole to attract the magnetic beads (nucleic acids are adsorbed on the magnetic beads) of the cracking solution in the nucleic acid extraction hole, the injector sucks the residual liquid except the magnetic beads, the residual liquid is transferred to the first reagent storage hole to be stored as waste liquid, and the magnet is far away from the nucleic acid extraction hole.

S64, rotating an injector to a third reagent storage hole of the card box main body, and sucking washing liquid in the third reagent storage hole by the injector through a third channel, wherein the third channel comprises a third liquid drainage channel and a third exhaust channel, the third liquid drainage channel is formed when a liquid transfer hole of the injector is communicated with the liquid drainage hole of the third reagent storage hole, and the third exhaust channel is formed when a gas transfer hole of the injector is communicated with an exhaust hole of the third reagent storage hole;

step S65, rotating the injector to the nucleic acid extraction hole of the cartridge body, wherein the injector transfers the washing solution in the sucked third reagent storage hole to the nucleic acid extraction hole through the nucleic acid channel;

specifically, after the injector rotationally moves to the third reagent storage hole, the injector sucks washing liquid in the third reagent storage hole through the third channel, then the injector rotates to the nucleic acid extraction hole, the injector transfers the washing liquid to the nucleic acid extraction hole, after the washing liquid is uniformly mixed, the magnet is close to the nucleic acid extraction hole, magnetic beads in reaction liquid in the nucleic acid extraction hole are sucked (nucleic acid is adsorbed on the magnetic beads and washed), the injector sucks residual liquid except the magnetic beads, the residual liquid is transferred to the second reagent storage hole to be stored as waste liquid, and the magnet is far away from the nucleic acid extraction hole.

Step S66, rotating the injector to a fourth reagent storage hole of the cartridge main body, sucking the eluent stored in the fourth reagent storage hole by the injector through a fourth channel, wherein the fourth channel comprises a fourth liquid drainage channel and a fourth exhaust channel when the liquid drainage hole of the injector is communicated with the liquid drainage hole of the fourth reagent storage hole, the fourth liquid drainage channel when the liquid drainage hole of the injector is communicated with the liquid drainage hole of the fourth reagent storage hole, and the fourth exhaust channel when the liquid drainage hole of the injector is communicated with the exhaust hole of the fourth reagent storage hole;

step S67, rotating the injector to the nucleic acid extraction hole of the cartridge body, and transferring the eluent in the absorbed fourth reagent storage hole to the nucleic acid extraction hole through the nucleic acid channel by the injector;

specifically, the elution liquid in the fourth reagent storage hole is transferred to the nucleic acid extraction hole through the nucleic acid channel by the injector, the magnetic beads in the elution liquid are absorbed by the magnet after uniform mixing, the nucleic acid can be eluted from the magnetic beads by the elution liquid, the elution liquid carrying the nucleic acid in the nucleic acid extraction hole is absorbed by the injector, the magnet is separated from the nucleic acid extraction hole, and the magnetic beads are remained in the nucleic acid extraction hole.

And S68, rotating the injector to a fifth reagent storage hole of the cartridge body, continuously sucking the PCR reaction liquid in the fifth reagent storage hole by the injector through a fifth channel, wherein the fifth channel is formed when the liquid transfer hole of the injector is communicated with the liquid discharge hole of the fifth reagent storage hole, the fifth channel comprises a fifth liquid discharge channel and a fifth air discharge channel, the fifth liquid discharge channel is formed when the liquid transfer hole of the injector is communicated with the liquid discharge hole of the fifth reagent storage hole, and the fifth air discharge channel is formed when the liquid transfer hole of the injector is communicated with the air discharge hole of the fifth reagent storage hole.

The PCR reaction solution is a high-fidelity PCR amplification premixed solution, and is suitable for PCR amplification and other PCR-related cloning and detection.

In other embodiments, the PCR reaction solution stored in the fifth reagent storage well may be pre-packaged in the PCR reaction well, and the fifth reagent storage well is omitted, so that after step S68, step S69 is directly performed, the liquid stored in the syringe is transferred to the PCR reaction well, mixing with the PCR reaction solution is achieved in the PCR reaction well, and detection of the sample is achieved in the PCR reaction well through real-time fluorescence quantification.

And S69, rotating the injector to the PCR reaction hole of the card box body, transferring the liquid in the injector to the PCR reaction hole through the PCR channel by the injector to realize the nucleic acid concentration monitoring of the sample by utilizing fluorescence quantification, wherein the PCR channel comprises a PCR liquid drainage channel and a PCR exhaust channel, the PCR liquid drainage channel is formed when the liquid drainage hole of the injector is communicated with the liquid drainage hole of the PCR reaction hole, and the PCR exhaust channel is formed when the gas drainage hole of the injector is communicated with the exhaust hole of the PCR reaction hole.

Specifically, when the syringe is rotated to align with the fifth reagent storage well, the syringe sucks the reagent in the fifth reagent storage well, and then rotates the syringe to the PCR reaction well of the cartridge body, the syringe and the PCR reaction well are in a communicated state, and the syringe transfers all the sucked liquid to the PCR reaction well through the PCR channel. FIG. 10 is a schematic view showing the state where the syringe communicates with the PCR reaction well of the cartridge body according to the embodiment of the present invention. Referring to FIG. 10, in a state where the injector 20 is in communication with the PCR reaction wells 130 of the cartridge body 10, all the liquids in the injector 20 (including the PCR reaction liquid and the nucleic acid-containing eluent) can be transferred to the PCR reaction wells through the PCR drainage channels in which the liquid transfer wells 222 are in communication with the drainage wells 132g in the PCR reaction wells; the gas in the syringe 20 can be transferred to the PCR reaction well through the PCR exhaust channel in which the gas transfer hole 221 communicates with the exhaust hole 131g in the PCR reaction well.

In this embodiment, the sample in the PCR reaction well can be subjected to fluorescent quantitative PCR detection by a fluorescent quantitative detection device to determine the concentration of nucleic acid in the PCR reaction well. Real-time fluorescent Quantitative PCR (Quantitative Real-time PCR) is a method for measuring the total amount of products after each Polymerase Chain Reaction (PCR) cycle by using fluorescent chemical substances in DNA amplification reaction.

It should be noted that, in the detection process, the included angle between the light source end of the fluorescence quantitative detection device and the light source of the detection end is 60 ° to 120 °. Ideally, the light source end of the fluorescence quantitative detection device and the light source of the detection end form an included angle of 90 °.

It is therefore clear that, the embodiment of the utility model provides an after transfer device rotary motion to assigned position, realized that the drain hole that moves liquid hole and a reagent storage structure on the transfer device lateral wall is linked together, after the intercommunication, can realize the liquid transfer between transfer device and the reagent storage structure, need not to expose sample or sample detection's in the testing process reagent in the air, and the pollution that probably causes when having avoided artifical the participation, for this reason, the utility model provides a card box can be at the inside transfer sample of the card box of same card box or be used for sample detection's reagent, has reduced the sample or has been used for sample detection's reagent to receive the probability of pollution in the testing process, can promote the detection efficiency of sample.

While various embodiments of the present invention have been described above, various alternatives described in the embodiments can be combined and cross-referenced without conflict to extend the variety of possible embodiments that can be considered disclosed and disclosed in the embodiments of the present invention.

Although the embodiments of the present invention have been disclosed, the present invention is not limited thereto. Various changes and modifications may be effected therein by one of ordinary skill in the pertinent art without departing from the scope or spirit of the present invention, and the scope of the present invention is defined by the appended claims.

Claims (15)

1. A cartridge, comprising:

the cartridge comprises a cartridge body and a plurality of reagent storage structures, wherein the cartridge body comprises a central hole groove and the reagent storage structures are used for providing space for a sample or a reagent for sample detection, the side walls of the reagent storage structures facing the central hole groove are provided with liquid discharge holes, and the liquid discharge holes of the reagent storage structures are positioned at the same height;

the transfer device is positioned in the central hole groove and can rotate in the central hole groove, the side wall of the transfer device is provided with a liquid transfer hole, the liquid transfer hole and the liquid discharge hole are positioned at the same height, and when the transfer device rotates and moves to a designated position, the liquid transfer hole is communicated with the liquid discharge hole of a reagent storage structure.

2. The cartridge of claim 1, wherein the reagent storage structure is further provided with vent holes disposed toward the side wall of the central well slot, the vent holes of a plurality of the reagent storage structures being located at the same height; the transfer device is also provided with a gas transfer hole; when the transfer device rotates and moves to a designated position, the air transfer hole is communicated with an exhaust hole of a reagent storage structure.

3. The cartridge of any of claims 1-2, wherein the plurality of reagent storage structures comprises: a nucleic acid extraction well, a PCR reaction well and a plurality of reagent storage wells.

4. The cartridge of claim 3, wherein the plurality of reagent storage wells are configured to contain different reagents, the plurality of reagent storage wells include a first reagent storage well configured to provide a receiving space for a sample, a second reagent storage well configured to store a lysis solution, a third reagent storage well configured to store a washing solution, a fourth reagent storage well configured to store an elution solution, and a fifth reagent storage well configured to store a PCR reaction solution; the nucleic acid extraction hole is used for providing a reaction space for nucleic acid extraction, and the PCR reaction hole is used for providing a reaction space for PCR reaction liquid.

5. The cartridge of claim 4, wherein the nucleic acid extraction well and the PCR reaction well are disposed on both sides of the central well groove, and the plurality of reagent storage wells are oppositely disposed with the nucleic acid extraction well and the PCR reaction well as a line of symmetry.

6. The cartridge of claim 1, wherein the transfer device is a syringe comprising a piston and a syringe barrel, the piston being inserted in the syringe barrel, the piston reciprocating within the syringe barrel to effect transfer of the sample or a reagent for sample testing.

7. The cartridge of claim 6, wherein the bottom of the syringe barrel is provided with a keyway keyed to a rotary motor of the cartridge body.

8. The cartridge of claim 7, wherein the injector further comprises a rubber layer disposed on an outer wall of the injector barrel.

9. The cartridge according to claim 3, wherein the nucleic acid extracting well comprises a nucleic acid well main body, and a first piping and a second piping connected to the nucleic acid well main body, the first piping being connected to the liquid drain hole of the nucleic acid extracting well, the second piping being connected to the gas drain hole of the nucleic acid extracting well; the nucleic acid well body protrudes from the plurality of reagent storage wells; the PCR reaction hole comprises a PCR reaction hole main body, a third pipeline and a fourth pipeline, wherein the third pipeline and the fourth pipeline are connected with the PCR reaction hole main body; the PCR reaction well main body protrudes from the plurality of reagent storage wells.

10. The cartridge of claim 3, wherein the plurality of reagent storage wells includes a first second reagent pooling well for simultaneously containing the sample and the lysate, a third reagent storage well for storing the wash solution, a fourth reagent storage well for storing the eluate, and a fifth reagent storage well for storing the PCR reaction solution; the nucleic acid extraction hole is used for providing a reaction space for nucleic acid extraction, and the PCR reaction hole is used for providing a reaction space for PCR reaction liquid.

11. The cartridge of claim 3, wherein the plurality of reagent storage wells includes a first reagent storage well, a third reagent storage well, a fourth reagent storage well and a fifth reagent storage well, wherein the nucleic acid extraction well is configured to provide a reaction space for a lysis solution and a nucleic acid extraction, the PCR reaction well is configured to provide a reaction space for a PCR reaction solution, the first reagent storage well is configured to provide an accommodation space for a sample, the third reagent storage well is configured to store a washing solution, the fourth reagent storage well is configured to store an elution solution, and the fifth reagent storage well is configured to store a PCR reaction solution.

12. The cartridge of claim 3, wherein the PCR reaction wells are packaged with PCR reaction solution, the nucleic acid extraction wells are configured to provide a reaction space for nucleic acid extraction, the plurality of reagent storage wells include a first reagent storage well, a second reagent storage well, a third reagent storage well, and a fourth reagent storage well, the first reagent storage well is configured to provide an accommodation space for a sample, the second reagent storage well is configured to store lysis solution, the third reagent storage well is configured to store washing solution, and the fourth reagent storage well is configured to store elution solution.

13. The cartridge of claim 3, further comprising a magnet, wherein the nucleic acid extraction well is provided with magnetic beads, and the magnet is fixed by a clamping mechanism and is disposed below the nucleic acid extraction well; the clamping mechanism is movable, and the magnet is based on the movement of the clamping mechanism and adsorbs magnetic beads in the nucleic acid extraction hole when being close to the nucleic acid extraction hole.

14. The cartridge of claim 3, further comprising a quantitative fluorescence detection device for monitoring the concentration of nucleic acid in the reaction solution in the PCR reaction well, wherein the light source end of the quantitative fluorescence detection device is disposed at an angle of 60 ° to 120 ° with respect to the light source of the detection end.

15. The cartridge of claim 2, wherein the air discharge hole has a height higher than that of the liquid discharge hole; the height of the gas transfer hole is higher than that of the liquid transfer hole.

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