CN218189727U - Device capable of realizing liquid transfer of stepped dosage liquid - Google Patents
Device capable of realizing liquid transfer of stepped dosage liquid Download PDFInfo
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- CN218189727U CN218189727U CN202222594528.9U CN202222594528U CN218189727U CN 218189727 U CN218189727 U CN 218189727U CN 202222594528 U CN202222594528 U CN 202222594528U CN 218189727 U CN218189727 U CN 218189727U
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Abstract
The utility model discloses a device that can realize that ladder dosage liquid moves liquid, include: a Z-direction linear displacement mechanism; a liquid transfer frame capable of performing linear motion in the Z direction under the drive of the Z-direction linear displacement mechanism; and at least two sets of mutually independent liquid-transfering components arranged on the liquid-transfering rack, wherein the liquid-transfering components comprise an air cylinder, a piston push plate connected with an output rod of the air cylinder and a plurality of injection pumps arranged on the piston push plate, an injection tube of the injection pump is fixedly arranged on the liquid-transfering rack, the upper end of a piston push rod of the injection pump is fixedly connected to the bottom of the piston push plate, and the piston push rod is driven by the piston push plate to move up and down relative to the injection tube so as to realize liquid-suction and liquid-injection actions. The utility model provides a device that can realize ladder dosage liquid moves liquid can divide the district to realize moving liquid of different dosages to can simplify the operation process, reduce the repetitive operation step, improve work efficiency, have fine application prospect.
Description
Technical Field
The utility model relates to a check out test set field, in particular to can realize ladder dosage liquid moves device of liquid.
Background
In biochemical experiments and clinical detection, a pipettor is one of the core devices in the whole experimental process, and the pipetting precision of the pipettor directly influences the whole experiment and detection results.
In the prior art, the liquid can only be pipetted in equal dosage by one-time liquid-absorbing spraying liquid, and in an actual experiment, the hole plate is often required to be subjected to stepped dilution, so that the liquid can only be pipetted in different dosages one by one, the operation process is more complicated, and the multiple operations have the probability of inaccurate liquid-transferring amount to a certain extent.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that to the not enough among the above-mentioned prior art, provide a device that can realize that ladder dosage liquid moves liquid.
In order to solve the technical problem, the utility model discloses a technical scheme is: an apparatus for enabling step-dose liquid pipetting, comprising:
a Z-direction linear displacement mechanism;
a liquid transfer frame capable of performing a linear motion in the Z direction by being driven by the Z-direction linear displacement mechanism;
the liquid transfer assembly comprises an air cylinder, a piston push plate connected with an output rod of the air cylinder and a plurality of injection pumps arranged on the piston push plate, injection tubes of the injection pumps are fixedly arranged on the liquid transfer rack, the upper ends of piston push rods of the injection pumps are fixedly connected to the bottom of the piston push plate, and the piston push plate drives the piston push rods to move up and down relative to the injection tubes to achieve liquid suction and injection actions.
Preferably, the liquid transferring frame is sequentially provided with a top plate, a middle supporting plate and a bottom plate from top to bottom; the air cylinder is arranged on the top plate, the piston push plate is movably arranged between the top plate and the middle support plate, the upper portion and the lower portion of the injection tube are fixedly connected with the middle support plate and the bottom plate respectively, and the bottom of the injection tube penetrates through the bottom plate and is connected with a hollow gun head rod which is used for being inserted into the liquid-transfer gun head in a matched mode.
Preferably, the device capable of realizing step dosage liquid pipetting further comprises a pipette head assembly arranged on the pipetting rack and used for disassembling the pipette heads on all the pipette head rods of all the pipetting assemblies at one time.
Preferably, the lance head withdrawing assembly comprises a lance head withdrawing plate which is arranged below the bottom plate in a vertically sliding manner, a reset connecting plate which is fixedly connected to the liquid moving frame and is positioned between the middle supporting plate and the bottom plate, and a plurality of lance head withdrawing driving mechanisms which are arranged between the lance head withdrawing plate and the reset connecting plate;
the gun withdrawing head plate is provided with a first sliding hole for the bottom of the injection tube to pass through in a sliding manner, and the diameter of the first sliding hole is smaller than the outer diameter of the top end of the liquid-transfering gun head.
Preferably, the gun head withdrawing driving mechanism comprises a linear bearing arranged on the middle supporting plate, a gun head withdrawing shaft movably inserted in the linear bearing and the upper end of the gun head withdrawing shaft is connected with the piston push plate, a shaft hole formed in the reset connecting plate and used for the gun head withdrawing shaft to pass through in a matching manner, a second slide hole formed in the bottom plate and positioned right below the shaft hole, a boss movably inserted in the second slide hole and the bottom of the boss is connected with the gun head withdrawing plate, and a reset spring connected between the boss and the reset connecting plate;
the gun withdrawing head shaft can downwards penetrate through the cavity in the middle of the return spring and is in contact with the boss below, so that the gun withdrawing head plate is pushed to downwards move.
Preferably, a plurality of gaskets are arranged between the bottom plate and the gun withdrawing head plate.
Preferably, four sets of pipetting assemblies are sequentially arranged on the pipetting rack along the Y direction.
Preferably, the Z-direction linear displacement mechanism includes a motor, a linear guide rail arranged along the Z direction, a slider arranged on the guide rail in a matching manner, and a synchronous belt driving mechanism for driving and connecting the motor and the slider, and the motor drives the slider to perform reciprocating movement in the Z direction through the synchronous belt driving mechanism;
the liquid transferring frame further comprises a driving connecting plate connected with the sliding block.
Preferably, the linear guide rail and the slide block both comprise two groups, and the synchronous belt driving mechanism comprises a driving wheel in driving connection with an output shaft of the motor, a first driven wheel in driving connection with the driving wheel through a first belt, a second driven wheel and a third driven wheel coaxially connected with the first driven wheel, a fourth driven wheel in driving connection with the second driven wheel through a second belt, and a fifth driven wheel in driving connection with the third driven wheel through a third belt;
one of the two groups of sliding blocks is connected with the second belt, and the other sliding block is connected with the third belt.
Preferably, the device capable of realizing step dosage liquid pipetting further comprises a base and a shell arranged on the base, and the Z-direction linear displacement mechanism is arranged inside the shell.
The utility model has the advantages that:
the utility model provides a device that can realize ladder dosage liquid moves liquid can divide the district to realize moving liquid of different dosages to can simplify the operation process, reduce the repetitive operation step, improve work efficiency, have fine application prospect.
Drawings
FIG. 1 is a schematic structural view of the device for transferring liquid in stepped dosage according to the present invention with the casing removed;
FIG. 2 is a schematic view of the overall structure of the device for transferring liquid in stepped dosage according to the present invention;
FIG. 3 is a schematic cross-sectional view of a perspective of the stepped liquid pipetting device of the present invention;
FIG. 4 is a schematic cross-sectional view of another perspective of the device for stepped liquid pipetting according to the present invention;
fig. 5 is a schematic structural view of the Z-direction linear displacement mechanism of the present invention.
Description of reference numerals:
1-Z direction linear displacement mechanism; 10, a motor; 11-linear guide; 12-a slide block; 13-synchronous belt drive mechanism; 130-driving wheel; 131-a first belt; 132 — a first driven wheel; 133 — a second driven wheel; 134 — third driven wheel; 135-a second belt; 136 — fourth driven wheel; 137 — a third belt; 138 — fifth driven wheel;
2-a liquid transferring rack; 20-a top plate; 21-middle support plate; 22-a base plate; 23-driving connecting plate;
3-a pipetting assembly; 30-a cylinder; 31-piston push plate; 32-a syringe pump; 33-an injection cartridge; 34-piston pusher rod; 35-a lance tip rod;
4, withdrawing the gun head assembly; 40, withdrawing the gun head plate; 41-reset connecting plate; 42-withdrawing the gun head driving mechanism; 43 — first slide hole; 44-a gasket; 420-linear bearing; 421-withdraw the gun head shaft; 422-shaft hole; 423 — second slide hole; 424-boss; 425-a return spring;
5, a base; 6-a housing; 7-pipette tips; 8, a microporous plate.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can implement the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
As shown in fig. 1 to 5, the device for pipetting a liquid in a step dosage of the present embodiment comprises:
a Z-direction linear displacement mechanism 1;
a liquid transfer frame 2 that can perform linear motion in the Z direction by being driven by the Z-direction linear displacement mechanism 1;
and at least two sets of mutually independent liquid-transfering components 3 arranged on the liquid-transfering rack 2, the liquid-transfering components 3 comprise an air cylinder 30, a piston push plate 31 connected with an output rod of the air cylinder 30 and a plurality of injection pumps 32 arranged on the piston push plate 31, injection tubes 33 of the injection pumps 32 are fixedly arranged on the liquid-transfering rack 2, the upper ends of piston push rods 34 of the injection pumps 32 are fixedly connected with the bottom of the piston push plate 31, and the piston push plate 31 drives the piston push rods 34 to move up and down relative to the injection tubes 33 so as to realize liquid-suction and liquid-injection actions.
Wherein, a top plate 20, a middle support plate 21 and a bottom plate 22 are sequentially arranged on the liquid-transferring rack 2 from top to bottom;
the air cylinder 30 is arranged on the top plate 20, the piston push plate 31 is movably arranged between the top plate 20 and the middle support plate 21, the upper part and the lower part of the injection tube 33 are fixedly connected with the middle support plate 21 and the bottom plate 22 respectively, and the bottom of the injection tube 33 penetrates through the bottom plate 22 and is connected with a hollow gun head rod 35 which is used for being inserted into the pipette head 7 in a matching mode.
In a preferred embodiment, the device capable of realizing step dosage liquid pipetting further comprises a pipette tip withdrawing assembly 4 which is arranged on the pipetting rack 2 and is used for unloading the pipette tips 7 on all the tip rods 35 of all the pipetting assemblies 3 at one time.
The gun head withdrawing assembly 4 comprises a gun head withdrawing plate 40 which is arranged below the bottom plate 22 in a vertically sliding manner, a resetting connecting plate 41 which is fixedly connected to the liquid transferring frame 2 and is positioned between the middle supporting plate 21 and the bottom plate 22, and a plurality of gun head withdrawing driving mechanisms 42 which are arranged between the gun head withdrawing plate 40 and the resetting connecting plate 41;
the withdrawal head plate 40 is provided with a first sliding hole 43 through which the bottom of the injection tube 33 can slide, and the diameter of the first sliding hole 43 is smaller than the outer diameter of the top end of the pipette tip 7.
The gun head withdrawing driving mechanism 42 comprises a linear bearing 420 arranged on the middle support plate 21, a gun head withdrawing shaft 421 movably inserted in the linear bearing 420 and the upper end of the gun head withdrawing shaft is connected with the piston push plate 31, a shaft hole 422 formed on the reset connecting plate 41 and through which the gun head withdrawing shaft 421 is matched, a second slide hole 423 formed on the bottom plate 22 and positioned right below the shaft hole 422, a boss 424 movably inserted in the second slide hole 423 and the bottom of the boss 424 connected with the gun head withdrawing plate 40, and a reset spring 425 connected between the boss 424 and the reset connecting plate 41;
the gun withdrawing shaft 421 can pass through the cavity in the middle of the return spring 425 downwards and contact with the boss 424 below to push the gun withdrawing head plate 40 to move downwards.
In the preferred embodiment, spacers 44 are also provided between the base plate 22 and the tip plate 40.
In a preferred embodiment, the Z-direction linear displacement mechanism 1 comprises a motor 10, a linear guide rail 11 arranged along the Z direction, a slide block 12 arranged on the guide rail in a matching way, and a synchronous belt driving mechanism 13 for driving and connecting the motor 10 and the slide block 12, wherein the motor 10 drives the slide block 12 to perform reciprocating movement in the Z direction through the synchronous belt driving mechanism 13; the pipetting rack 2 further comprises a drive link plate 23 connected to the slide 12.
In a further preferred embodiment, the linear guide 11 and the sliding block 12 each include two sets, and the synchronous belt driving mechanism 13 includes a driving wheel 130 drivingly connected to the output shaft of the motor 10, a first driven wheel 132 drivingly connected to the driving wheel 130 through a first belt 131, a second driven wheel 133 and a third driven wheel 134 coaxially connected to the first driven wheel 132, a fourth driven wheel 136 drivingly connected to the second driven wheel 133 through a second belt 135, and a fifth driven wheel 138 drivingly connected to the third driven wheel 134 through a third belt 137; one slider 12 of the two sets of sliders 12 is connected to a second belt 135 and the other slider 12 is connected to a third belt 137.
The working principle of the Z-direction linear displacement mechanism 1 is as follows: the motor 10 drives the first driven wheel 132 to rotate through the driving wheel 130, and then drives the second driven wheel 133 and the third driven wheel 134 to rotate, so that the second belt 135 and the third belt 137 move, thereby driving the two sets of sliders 12 to perform synchronous movement in the Z direction, and finally driving the entire pipetting rack 2 to perform reciprocating movement in the Z direction through the sliders 12.
In the preferred embodiment, the device capable of realizing step dosage liquid pipetting further comprises a base 5 and a shell 6 arranged on the base 5, and the Z-direction linear displacement mechanism 1 is arranged inside the shell 6.
In this embodiment, four sets of liquid-transfering assemblies 3 have set gradually along the Y direction on the liquid-transfering rack 2, move back rifle head actuating mechanism 42 and also include four, set up on moving back rifle head plate 40 and four angles between the connecting plate 41 that resets, following this is taken as the example to the utility model discloses a theory of operation explains:
1. loading a pipette tip 7:
the pipette head box loaded with the pipette heads 7 is placed under the pipette stand 2, and the Z-direction linear displacement mechanism 1 drives the pipette stand 2 to integrally move downwards, so that the pipette head rods 35 are inserted into the pipette heads 7 under certain pressure, and the pipette heads 7 are loaded on the pipette head rods 35; the size of the gun head rod 35 is matched with that of the liquid-transfer gun head 7, so that stable connection and good sealing can be guaranteed;
2-1, when carrying out equal liquid absorption and injection: the Z-direction linear displacement mechanism 1 drives the liquid transfer rack 2 to move upwards integrally, the lower gun head box is replaced by a reagent box, and four air cylinders 30 in the four liquid transfer assemblies 3 respectively drive corresponding piston push plates 31 to move downwards to drive piston push rods 34 connected with the piston push plates to move downwards to discharge air in injection cylinders 33; then the Z-direction linear displacement mechanism 1 drives the liquid transfer rack 2 to move downwards integrally, the liquid transfer gun head 7 extends into the reagent, then the four air cylinders 30 drive the corresponding piston push rods 34 to move upwards in an equivalent manner, and the reagent is sucked into the liquid transfer gun head 7 by utilizing pressure difference;
during liquid injection, the Z-direction linear displacement mechanism 1 drives the liquid transfer frame 2 to move upwards integrally, the lower kit is replaced by the micro-porous plate 8, the liquid transfer frame 2 moves downwards integrally to enable the liquid transfer gun head 7 to extend into the micro-porous plate 8, and the four cylinders 30 drive the corresponding piston push plates 31 to move downwards by the same stroke as that during liquid absorption respectively, so that equivalent liquid absorption and injection are completed;
2-2, when gradient liquid suction and injection are carried out: the difference from 2-1 is that, during pipetting, the four cylinders 30 respectively drive the corresponding piston push plates 31 to move downwards by a stroke a, b, c and d, for example, a < b < c < d, so as to aspirate unequal amounts of reagents; during liquid injection, the four cylinders 30 respectively drive the corresponding piston push plates 31 to move upwards by a stroke a, a stroke b, a stroke c and a stroke d, and unequal amounts of reagents are injected, so that gradient liquid absorption and injection are realized, and a sample with gradient dilution is obtained;
3. unloading the pipette tip 7: the two cylinders 30 at the two ends work synchronously to drive the piston plate to move downwards to drive the four gun head withdrawing shafts 421 to move downwards synchronously, and the bottoms of the gun head withdrawing shafts 421 penetrate through the cavity in the middle of the return spring 425 and press the bosses 424 below downwards, so that the gun head withdrawing plate 40 is pushed to move downwards; because the diameter of the first slide hole 43 formed in the gun withdrawing head plate 40 is smaller than the outer diameter of the top end of the pipetting gun heads 7, the gun withdrawing head plate 40 generates downward pressure on all the pipetting gun heads 7, so that the pipetting gun heads 7 are separated from the gun head rod 35, and all the pipetting gun heads 7 are detached at one time; the air cylinder 30 then drives the piston plate upward, and the muzzle plate 40 is returned upward under the pulling force of the return spring 425.
It should be understood that, since the distance between the gun withdrawing shaft 421 and the boss 424 is suitable, the distance that the piston plate moves downwards does not make the gun withdrawing shaft 421 contact the boss 424 during the liquid sucking and injecting process, that is, the gun withdrawing head plate 40 does not move relative to the pipette head 7 during the liquid sucking and injecting process.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship indicated based on the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
While the embodiments of the invention have been disclosed above, it is not limited to the applications listed in the description and the embodiments, which are fully applicable in all kinds of fields where the invention is suitable, and further modifications may readily be made by those skilled in the art, and the invention is therefore not limited to the specific details without departing from the general concept defined by the claims and the scope of equivalents.
Claims (10)
1. An apparatus for enabling step-dose liquid pipetting, comprising:
a Z-direction linear displacement mechanism;
a liquid transfer frame capable of performing linear motion in the Z direction by being driven by the Z-direction linear displacement mechanism;
the liquid transfer assembly comprises an air cylinder, a piston push plate connected with an output rod of the air cylinder and a plurality of injection pumps arranged on the piston push plate, injection tubes of the injection pumps are fixedly arranged on the liquid transfer frame, the upper ends of piston push rods of the injection pumps are fixedly connected to the bottom of the piston push plate, and the piston push plate drives the piston push rods to move up and down relative to the injection tubes to achieve liquid suction and injection actions.
2. The device capable of realizing step-dose liquid pipetting of claim 1, wherein the pipetting rack is provided with a top plate, a middle support plate and a bottom plate from top to bottom in sequence;
the air cylinder is arranged on the top plate, the piston push plate is movably arranged between the top plate and the middle support plate, the upper portion and the lower portion of the injection tube are fixedly connected with the middle support plate and the bottom plate respectively, and the bottom of the injection tube penetrates through the bottom plate and is connected with a hollow gun head rod which is used for being inserted into the liquid-transfer gun head in a matched mode.
3. The apparatus for stepped liquid pipetting according to claim 2, further comprising a pipette tip assembly disposed on the pipetting rack for removing the pipette tips from all the tip rods of all the pipetting assemblies at a time.
4. A device capable of achieving stepped dosage liquid pipetting, as recited in claim 3, wherein the pipette tip withdrawing assembly comprises a pipette tip withdrawing plate slidably disposed below the bottom plate, a reset connecting plate fixedly connected to the pipetting frame and disposed between the middle support plate and the bottom plate, and a plurality of pipette tip withdrawing driving mechanisms disposed between the pipette tip withdrawing plate and the reset connecting plate;
the gun withdrawing head plate is provided with a first sliding hole for the bottom of the injection tube to pass through in a sliding manner, and the diameter of the first sliding hole is smaller than the outer diameter of the top end of the liquid-transfering gun head.
5. The device capable of achieving liquid transfer of stepped doses according to claim 4, wherein the tip withdrawing driving mechanism comprises a linear bearing arranged on the middle support plate, a tip withdrawing shaft movably inserted into the linear bearing and having an upper end connected with the piston push plate, a shaft hole formed in the reset connecting plate and allowing the tip withdrawing shaft to pass through in a matching manner, a second slide hole formed in the bottom plate and located right below the shaft hole, a boss movably inserted into the second slide hole and having a bottom connected with the tip withdrawing plate, and a reset spring connected between the boss and the reset connecting plate;
the gun withdrawing head shaft can downwards penetrate through the cavity in the middle of the return spring and is in contact with the boss below, so that the gun withdrawing head plate is pushed to downwards move.
6. The device capable of achieving step dosing liquid pipetting of claim 5, wherein a plurality of gaskets are further arranged between the bottom plate and the pipette head plate.
7. Device for stepped liquid pipetting according to any of claims 1 to 6, characterized in that four sets of pipetting units are arranged in succession in the Y direction on the pipetting rack.
8. The apparatus for achieving step-dose liquid pipetting according to claim 1, wherein the Z-direction linear displacement mechanism comprises a motor, a linear guide rail arranged along the Z-direction, a slide block arranged on the guide rail in a matching way, and a synchronous belt driving mechanism for driving and connecting the motor and the slide block, and the motor drives the slide block to reciprocate in the Z-direction through the synchronous belt driving mechanism;
the liquid transferring frame further comprises a driving connecting plate connected with the sliding block.
9. A device capable of achieving step dosage liquid pipetting as recited in claim 8, wherein the linear guide rails and the slide blocks are comprised of two sets, the synchronous belt driving mechanism comprises a driving wheel drivingly connected with the output shaft of the motor, a first driven wheel drivingly connected with the driving wheel through a first belt, a second driven wheel and a third driven wheel coaxially connected with the first driven wheel, a fourth driven wheel drivingly connected with the second driven wheel through a second belt, and a fifth driven wheel drivingly connected with the third driven wheel through a third belt;
one of the two groups of sliding blocks is connected with the second belt, and the other sliding block is connected with the third belt.
10. The device capable of step dose liquid pipetting of claim 9, further comprising a base and a housing disposed on said base, said Z-direction linear displacement mechanism being disposed inside said housing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222594528.9U CN218189727U (en) | 2022-09-29 | 2022-09-29 | Device capable of realizing liquid transfer of stepped dosage liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222594528.9U CN218189727U (en) | 2022-09-29 | 2022-09-29 | Device capable of realizing liquid transfer of stepped dosage liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218189727U true CN218189727U (en) | 2023-01-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222594528.9U Active CN218189727U (en) | 2022-09-29 | 2022-09-29 | Device capable of realizing liquid transfer of stepped dosage liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218189727U (en) |
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- 2022-09-29 CN CN202222594528.9U patent/CN218189727U/en active Active
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