CN218174990U

CN218174990U - Cell culture equipment

Info

Publication number: CN218174990U
Application number: CN202222238375.4U
Authority: CN
Inventors: 罗玉龙; 胡璧梁; 彭倩
Original assignee: Chutian Siweikang Gene Technology Changsha Co ltd
Current assignee: Chutian Siweikang Gene Technology Changsha Co ltd
Priority date: 2022-08-23
Filing date: 2022-08-23
Publication date: 2022-12-30
Anticipated expiration: 2032-08-23

Abstract

The utility model discloses a cell culture equipment, including the box that is equipped with a body, be located the wind-guiding cover in the box, the fan subassembly, motor and disposable culture container, motor output shaft is located one side of box, the opposite side of box is equipped with detachable rotating assembly, the one end of disposable culture container can be dismantled with the transport axle of motor and be connected, the other end is equipped with the rotary joint who is connected with rotating assembly, the rotary joint end of disposable culture container is equipped with the infusion pipeline, be equipped with the pinch valve of installation infusion pipeline on the box, delivery pump and preheating part, be equipped with transparent window on the disposable culture container, be equipped with the optical vision part through the transparent window monitoring cell culture condition on the box. The cell culture equipment has the advantages of realizing the rotation adjustment of the disposable culture container, being convenient for the replacement of the disposable culture container, being convenient for the cleaning and maintenance of the interior of the box body, and the like.

Description

Cell culture equipment

Technical Field

The utility model relates to a food, pharmaceutical packaging mechanical equipment technical field especially relate to a cell culture equipment.

Background

With the continuous improvement of human medical technology, gene and cell sequencing technology is mature and popularized, and the demands on cell culture equipment and automatic production are greatly increased. The existing cell culture equipment generally comprises a box body, an air guide sleeve arranged in the box body and a disposable culture container arranged in the air guide sleeve. The disposable culture container and the air guide sleeve in the box body of the existing cell culture equipment are fixedly arranged, so that the disposable culture container and the air guide sleeve are inconvenient to disassemble and cause difficulty in cleaning the box body.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to overcome prior art not enough, provide one kind and can realize disposable culture vessel rotation regulation, the disposable culture vessel of being convenient for again is changed, the inside cell culture equipment who washs and maintains of box of still being convenient for.

In order to solve the technical problem, the utility model discloses a following technical scheme:

the utility model provides a cell culture equipment, is including the box that is equipped with the door body, be located guide sleeve, fan subassembly, motor and the disposable culture container of box, the motor output shaft is located one side of box, the opposite side of box is equipped with detachable rotating assembly, the one end of disposable culture container can be dismantled with the delivery shaft of motor and be connected, and the other end is equipped with the rotary joint who is connected with rotating assembly, the rotary joint end of disposable culture container is equipped with the infusion pipeline, be equipped with pinch valve, the delivery pump and the preheating part of installation infusion pipeline on the box, be equipped with transparent window on the disposable culture container, be equipped with the optical vision part through the transparent window monitoring cell culture condition on the box.

As a further improvement of the above technical solution:

the disposable culture container is provided with a butt joint component which is connected with a conveying shaft spline of a motor.

The box body is provided with a clamping piece, and the rotating assembly is clamped on the clamping piece.

The one end of the buckle spare of rotating assembly orientation is equipped with joint portion, the buckle spare is equipped with the draw-in groove that runs through to the side towards rotating assembly's one end, joint portion buckle is in the draw-in groove, the side opening department of draw-in groove is equipped with detachably and seals the part.

The rotating assembly comprises a fixed shaft and a bearing, the rotating joint is coaxially connected with the fixed shaft through the bearing, and the clamping portion is arranged on the fixed shaft.

And a flange plate is arranged on the inner wall of the box body, and one end of the buckle piece, which is far away from the rotating assembly, is connected with a spline of the flange plate and is in axial butt joint with the flange plate through a butt joint magnet.

The butt joint assembly comprises a rotating shaft and a butt joint shaft, the rotating shaft is fixed on the disposable culture container, one end of the butt joint shaft is in spline connection with the conveying shaft of the motor and is in axial butt joint with the conveying shaft of the motor through a connecting magnet, and the other end of the butt joint shaft is in detachable butt joint with the rotating shaft.

The periphery of axis of rotation is equipped with spacing annular, be equipped with the elasticity ball in the spacing annular, the axis of rotation is located to the butt joint axle sleeve, the elasticity ball supports tightly between butt joint axle and axis of rotation, just butt joint axle and axis of rotation pass through the synchronous swivelling joint of round pin spare.

The air guide sleeve is positioned in the box body and is a square sleeve with an open bottom side and an open front side, the bottom and the side of the air guide sleeve are respectively provided with a separation column, so that the air guide sleeve is separated from the inner wall of the box body, the back of the air guide sleeve is provided with an air outlet, the fan assembly is in butt joint with the air outlet, and a water tank is arranged below the air guide sleeve in the box body.

The optical visual component is arranged at the bottom of the box body, a refrigerating box is arranged on one side of the box body, and the preheating component is positioned above the refrigerating box.

Compared with the prior art, the utility model has the advantages of:

the preheating component of the cell culture equipment of the utility model preheats the disposable culture container through the delivery pump and the infusion pipeline; the transfusion pipeline is installed through a pinch valve, so that the installation is convenient; the cell culture conditions in the disposable culture vessel were monitored by optical vision means. The one end of disposable culture container can be dismantled with the transport axle of motor and be connected, and the other end passes through rotary joint and is connected with the rotating assembly to can dismantle with the box through the rotating assembly and be connected, can realize disposable culture container's rotation regulation, the change of the disposable culture container of being convenient for again, and still be convenient for inside washing of box and maintenance.

Drawings

FIG. 1 is a schematic perspective view of the cell culture apparatus of the present invention.

FIG. 2 is a schematic view of the main structure of the cell culture apparatus of the present invention.

FIG. 3 is a schematic diagram of a side-sectional structure of the cell culture apparatus of the present invention.

Fig. 4 is an enlarged view of a in fig. 2.

Fig. 5 is an enlarged view of B in fig. 2.

FIG. 6 is a schematic view of a partial structure of the cell culture apparatus of the present invention.

Fig. 7 is a partial structural view of fig. 6.

FIG. 8 is a schematic structural view of the air guide sleeve of the cell culture apparatus of the present invention.

The reference numerals in the figures denote:

1. a box body; 11. a water tank; 12. an optical vision component; 13. a refrigerating box; 14. a preheating component; 15. a delivery pump; 2. a wind guide sleeve; 21. isolating the column; 22. an air outlet; 3. a disposable culture vessel; 4. a motor; 5. a door body; 6. a fan assembly; 7. a docking assembly; 71. a rotating shaft; 711. a limiting ring groove; 712. an elastic ball; 72. a butt joint shaft; 73. connecting a magnet; 74. a pin member; 8. a rotating assembly; 81. a rotary joint; 82. a fixed shaft; 83. a bearing; 84. a clamping part; 9. a fastener; 91. a card slot; 92. a flange plate; 93. and (6) butting the magnets.

Detailed Description

The invention will be described in further detail with reference to the drawings and specific examples.

As used in this disclosure and in the claims, the terms "a," "an," "the," and/or "the" are not intended to be inclusive in the singular, but rather are inclusive in the plural unless the context clearly dictates otherwise. The use of "first," "second," and the like in this disclosure is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. Likewise, the word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect.

Fig. 1 to 8 show an embodiment of the cell culture apparatus of the present invention, the cell culture apparatus includes a box 1 provided with a door 5, a wind guide sleeve 2 located in the box 1, a fan assembly 6, a motor 4 and a disposable culture container 3, an output shaft of the motor 4 is located at one side of the box 1, the other side of the box 1 is provided with a detachable rotating assembly 8, one end of the disposable culture container 3 is detachably connected with a conveying shaft of the motor 4, the other end is provided with a rotating joint 81 connected with the rotating assembly 8, a rotating joint 81 end of the disposable culture container 3 is provided with a transfusion pipeline, a pinch valve for installing the transfusion pipeline is provided on the box 1, a conveying pump 15 and a preheating component 14, a transparent window is provided on the disposable culture container 3, and an optical vision component 12 for monitoring the cell culture condition through the transparent window is provided on the box 1.

In the cell culture equipment, a preheating part 14 preheats a disposable culture container 3 through a delivery pump 15 and a transfusion pipeline; the transfusion pipeline is installed through a pinch valve, so that the installation is convenient; the cell culture in the disposable culture vessel 3 is monitored by the optical vision unit 12. One end of the disposable culture container 3 is detachably connected with a conveying shaft of the motor 4, the other end of the disposable culture container is connected with the rotating component 8 through the rotating joint 81, and the rotating component 8 is detachably connected with the box body 1, so that the rotation adjustment of the disposable culture container 3 can be realized, the replacement of the disposable culture container 3 is facilitated, and the cleaning and maintenance of the interior of the box body 1 are facilitated.

In this embodiment, the disposable culture container 3 is provided with a docking assembly 7, and the docking assembly 7 is in spline connection with the conveying shaft of the motor 4. The butt joint component 7 is connected with the conveying shaft of the motor 4 through a spline, so that synchronous rotation can be realized, and axial separation is facilitated.

In this embodiment, as shown in fig. 5 and 7, the case 1 is provided with a fastener 9, and the rotating component 8 is fastened on the fastener 9, so as to be convenient for disassembly and assembly. Specifically, the one end of the buckle 9 that rotating assembly 8 faced is equipped with joint portion 84, and buckle 9 is equipped with the draw-in groove 91 that runs through to the side towards the one end of rotating assembly 8, and joint portion 84 buckle is in draw-in groove 91, and the side opening department of draw-in groove 91 is equipped with detachably and seals the part. When the rotary component 8 is detached, the sealing part at the opening of the side of the clamping groove 91 is detached, and then the clamping part 84 is moved out from the opening of the side of the clamping groove 91, so that the connection between the rotary component 8 and the clamping part 9 can be released; during the installation, insert joint portion 84 from the side opening of draw-in groove 91, seal the part again and install in draw-in groove 91 side opening department can, easy dismounting.

In this embodiment, as shown in fig. 5, the rotating assembly 8 includes a fixed shaft 82 and a bearing 83, the rotating joint 81 is coaxially connected with the fixed shaft 82 through the bearing 83, and the clamping portion 84 is disposed on the fixed shaft 82. The rotary joint 81 and the fixed shaft 82 are connected by a bearing 83 to satisfy a rotary motion relative to each other. The whole structure is simple, and the manufacture is convenient. Specifically, one end of the rotary joint 81 is fixedly connected to the disposable culture vessel 3, and the other end is coaxially connected to the fixed shaft 82 via a bearing 83.

In this embodiment, a flange 92 is disposed on the inner wall of the box 1, and one end of the fastening member 9 away from the rotating component 8 is splined to the flange 92 and is axially abutted by the abutting magnet 93. The clamping part 9 is connected with the flange plate 92 through splines, so that synchronous rotation can be realized, and axial separation is facilitated. And the butt joint department of buckle spare 9 and ring flange 92 is equipped with butt joint magnet 93, realizes axial butt joint through butt joint magnet 93, improves the stability of axial connection.

In this embodiment, the docking assembly 7 includes a rotating shaft 71 and a docking shaft 72, the rotating shaft 71 is fixed to the disposable culture container 3, one end of the docking shaft 72 is spline-coupled to the transport shaft of the motor 4 and is axially docked with the transport shaft of the motor 4 by a connection magnet 73, and the other end is detachably docked with the rotating shaft 71.

In this embodiment, as shown in fig. 4 and fig. 6, a limiting ring groove 711 is disposed on the outer periphery of the rotating shaft 71, an elastic ball 712 is disposed in the limiting ring groove 711, the abutting shaft 72 is sleeved on the rotating shaft 71, the elastic ball 712 is tightly abutted between the abutting shaft 72 and the rotating shaft 71, and the abutting shaft 72 and the rotating shaft 71 are synchronously and rotatably connected through a pin 74. The butt joint shaft 72 and the rotating shaft 71 are tightly connected through the elastic ball 712, so that the connection stability of the butt joint shaft 72 and the rotating shaft is improved, and the butt joint shaft and the rotating shaft are convenient to axially separate. When the butt joint shaft 72 needs to be separated from the rotating shaft 71, the pin piece 74 is pulled out, and then the butt joint shaft 72 and the rotating shaft 71 are pulled in the axial direction in the reverse direction to realize separation of the two.

In this embodiment, as shown in fig. 1, 2, 3, and 8, the air guide sleeve 2 is located in the box body 1, the air guide sleeve 2 is a square sleeve with open bottom and front sides, the bottom and the side of the air guide sleeve 2 are both provided with separating columns 21 to separate the air guide sleeve 2 from the inner wall of the box body 1, the back of the air guide sleeve 2 is provided with an air outlet 22, the fan assembly 6 is in butt joint with the air outlet 22, and the water tank 11 is located below the air guide sleeve 2 in the box body 1. The bottom of the air guide sleeve 2 is supported at the bottom in the box body 1 through a separation column 21, and two sides of the air guide sleeve are separated from the inner wall of the box body 1 through the separation column 21. The air guide sleeve 2 is not fixedly connected with the box body 1, so that the installation and the taking are convenient, and the cleaning and the maintenance in the box body 1 are convenient.

In this embodiment, the optical visual unit 12 is provided at the bottom of the casing 1, the refrigerating box 13 is provided at one side of the casing 1, and the preheating unit 14 is located above the refrigerating box 13.

The preheating part 14 preheats the disposable culture container 3 through the delivery pump 15 and the infusion pipeline, specifically, the preheating part 14 preheats the flowing culture medium of the infusion pipeline, and the preheated culture medium flows into the disposable culture container 3 again. Is the medium flowing in the tubing. The transparent window of the box body 1 is arranged on the door body 5, so that an operator can conveniently observe the condition in the box at any time, and the door opening and defogging function is realized; the optical vision part 12 is arranged at the bottom of the box body 1, cells need to be input into a flowing cuvette in the optical vision part 12 through a pump, after the cells are settled for a period of time, the cells are photographed, the cell culture amplification density and the cell growth condition can be monitored and distinguished through photographing and AI analysis on line, and a control center prepares a cell growth curve in the whole cell culture process according to the monitoring data.

Although the present invention has been described with reference to the preferred embodiments, it is not intended to be limited thereto. The technical solution of the present invention can be used by anyone skilled in the art to make many possible variations and modifications, or to modify equivalent embodiments, without departing from the scope of the present invention, using the technical content disclosed above. Therefore, any simple modification, equivalent change and modification made to the above embodiments by the technical entity of the present invention should fall within the protection scope of the technical solution of the present invention.

Claims

1. A cell culture apparatus, characterized by: including box (1) that is equipped with the door body (5), be located wind-guiding cover (2) of box (1), fan subassembly (6), motor (4) and disposable culture container (3), motor (4) output shaft is located one side of box (1), the opposite side of box (1) is equipped with detachable rotating assembly (8), the one end of disposable culture container (3) can be dismantled with the delivery shaft of motor (4) and be connected, and the other end is equipped with rotary joint (81) and be connected with rotating assembly (8), the rotary joint (81) end of disposable culture container (3) is equipped with the transfusion tube way, be equipped with pinch valve, delivery pump (15) and preheating part (14) of installation transfusion tube way on box (1), be equipped with transparent window on disposable culture container (3), be equipped with optical vision part (12) through the transparent window monitoring cell culture condition on box (1).

2. The cell culture apparatus of claim 1, wherein: the disposable culture container (3) is provided with a butt joint component (7), and the butt joint component (7) is in spline connection with a conveying shaft of the motor (4).

3. The cell culture apparatus of claim 2, wherein: the box body (1) is provided with a clamping piece (9), and the rotating assembly (8) is clamped on the clamping piece (9).

4. The cell culture apparatus of claim 3, wherein: one end of buckle spare (9) of rotating assembly (8) orientation is equipped with joint portion (84), buckle spare (9) are equipped with draw-in groove (91) of running through to the side towards the one end of rotating assembly (8), joint portion (84) buckle is in draw-in groove (91), the side opening department of draw-in groove (91) is equipped with detachably and seals the part.

5. The cell culture apparatus of claim 4, wherein: the rotating assembly (8) comprises a fixed shaft (82) and a bearing (83), the rotating joint (81) is coaxially connected with the fixed shaft (82) through the bearing (83), and the clamping part (84) is arranged on the fixed shaft (82).

6. The cell culture apparatus of claim 4, wherein: and a flange plate (92) is arranged on the inner wall of the box body (1), and one end, far away from the rotating assembly (8), of the clamping piece (9) is connected with the flange plate (92) through splines and is axially butted through a butting magnet (93).

7. The cell culture apparatus of claim 2, wherein: the butt joint assembly (7) comprises a rotating shaft (71) and a butt joint shaft (72), the rotating shaft (71) is fixed on the disposable culture container (3), one end of the butt joint shaft (72) is in spline connection with the conveying shaft of the motor (4) and is in axial butt joint with the conveying shaft of the motor (4) through a connecting magnet (73), and the other end of the butt joint shaft is in detachable butt joint with the rotating shaft (71).

8. The cell culture apparatus of claim 7, wherein: the periphery of axis of rotation (71) is equipped with spacing annular (711), be equipped with elasticity ball (712) in spacing annular (711), axis of rotation (71) is located to butt joint axle (72) cover, elasticity ball (712) support tightly between butt joint axle (72) and axis of rotation (71), just butt joint axle (72) and axis of rotation (71) are through round pin spare (74) synchronous revolution connection.

9. The cell culture apparatus of any one of claims 1 to 8, wherein: the air guide sleeve (2) is located in the box body (1), the air guide sleeve (2) is a square sleeve with the bottom side and the front side open, the bottom and the side portion of the air guide sleeve (2) are respectively provided with a separation column (21), so that the air guide sleeve (2) is separated from the inner wall of the box body (1), the back of the air guide sleeve (2) is provided with an air outlet (22), the fan assembly (6) is in butt joint with the air outlet (22), and a water tank (11) is arranged below the air guide sleeve (2) in the box body (1).

10. The cell culture apparatus of any one of claims 1 to 8, wherein: the optical visual component (12) is arranged at the bottom of the box body (1), a refrigerating box (13) is arranged on one side of the box body (1), and the preheating component (14) is positioned above the refrigerating box (13).