CN218115337U - Protein purification device - Google Patents
Protein purification device Download PDFInfo
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- CN218115337U CN218115337U CN202222663837.7U CN202222663837U CN218115337U CN 218115337 U CN218115337 U CN 218115337U CN 202222663837 U CN202222663837 U CN 202222663837U CN 218115337 U CN218115337 U CN 218115337U
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- seat hole
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- elution
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- protein purification
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Abstract
The utility model discloses a protein purification device, which comprises a fermentation tank, wherein the fermentation tank is communicated with the bottom of an ultrasonic crushing device through a first liquid pump, a continuous filtering device is arranged below a liquid outlet at the upper part of the ultrasonic crushing device, the continuous filtering device is communicated with a buffer tank, the buffer tank is respectively communicated with a plurality of continuous purification mechanisms through a plurality of filtering stock solution pipes, and a second liquid pump is arranged on each filtering stock solution pipe; the continuous purification mechanism comprises a first rotating device, the first rotating device is connected with a porous resin column frame, and an adsorption station seat hole, an elution station seat hole and a cleaning station seat hole are sequentially connected to the porous resin column frame along the circumferential direction; and the adsorption station seat hole, the elution station seat hole and the cleaning station seat hole are all fixed with porous resin adsorption columns. The utility model discloses can realize that the thallus is broken, is filtered in the protein production, the albumen adsorbs, and elution and the continuous automation who washs the adsorption column go on, provide the efficiency of high protein purification.
Description
Technical Field
The utility model relates to a PCB board processing field especially relates to a protein purification device.
Background
Many proteins for medical or scientific purposes need to be purified before they can be used, for example, widely used cloned antibodies and the like. However, in the current protein purification, such as monoclonal antibody purification, it is usually necessary to break the carrier bacteria to release the protein therein, then centrifuge and precipitate to obtain the supernatant, pass the supernatant through a resin adsorption column to adsorb the target protein, and then elute with the eluent, thereby obtaining the single purified protein. However, the above steps are now performed manually, resulting in slower efficiency and thus need to be improved.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides a protein purification device. The utility model discloses can realize that the thallus is broken, is filtered in the protein production, the albumen adsorbs, and elution and the continuous automation who washs the adsorption column go on, provide the efficiency of high protein purification.
The purpose of the utility model is realized through the following technical scheme:
a protein purification device comprises a fermentation tank, wherein the fermentation tank is communicated with the bottom of an ultrasonic crushing device through a first liquid pump, a continuous filtering device is arranged below a liquid outlet at the upper part of the ultrasonic crushing device, the continuous filtering device is communicated with a buffer tank, the buffer tank is respectively communicated with a plurality of continuous purification mechanisms through a plurality of filtering stock solution pipes, and a second liquid pump is arranged on the filtering stock solution pipe; the continuous purification mechanism comprises a first rotating device, the first rotating device is connected with a porous resin column frame, and an adsorption station seat hole, an elution station seat hole and a cleaning station seat hole are sequentially connected to the porous resin column frame along the circumferential direction; porous resin adsorption columns are fixed on the adsorption station seat hole, the elution station seat hole and the cleaning station seat hole; an elution liquid pipe is arranged above the seat hole of the elution station, and an elution liquid box is communicated above the elution liquid pipe; a cleaning liquid pipe is arranged above the cleaning station seat hole and communicated with a deionized water tank; the elution liquid pipe and the cleaning liquid pipe are both provided with electromagnetic valves; a filtering raw liquid pipe is arranged above the adsorption station seat hole.
In a further improvement, the ultrasonic crushing device comprises a crushing pipe, and an ultrasonic generator is arranged along the length direction of the crushing pipe.
In a further improvement, the crushing pipe is internally provided with a spiral fan blade.
The continuous filtering device comprises a V-shaped rotary drum, the middle part of the rotary drum is a porous filtering area, and the bottom of the rotary drum is connected with a second rotating device; a filtrate collecting shell is covered on the periphery of the rotary drum, and the inner wall of the filtrate collecting shell is connected with a second rotating device through a support rod; the bottom of the filtrate collecting shell is communicated with a buffer tank; the filter residue collecting shell is covered with a filter residue collecting shell, a retaining ring matched with the top of the rotary drum is formed in the top of the filter residue collecting shell in a protruding mode, a discharge hole is formed in the bottom of the filter residue collecting shell, and the filter residue collecting shell is connected with the filter residue collecting shell through a support rod.
In a further improvement, flow meters are arranged on the elution liquid pipe, the cleaning liquid pipe and the filtering raw liquid pipe.
In a further improvement, the first rotating device is a motor, and the second rotating device is a rotating cylinder or a servo motor.
In a further improvement, liquid receiving basins are respectively arranged below the porous resin adsorption columns of the adsorption station seat hole, the elution station seat hole and the cleaning station seat hole; a material basin is arranged below the filtrate collecting shell.
In a further improvement, a limiting ring is raised in the middle of the porous resin adsorption column.
The beneficial effects of the utility model reside in that:
1. the utility model discloses can realize adsorbing, elution and clear continuous cycle go on, improve the purification efficiency of protein.
2. The utility model discloses a continuous filtration compares ordinary filter-pressing separation, has saved the time of clearance filter residue, has improved protein purification's continuity, has improved its production efficiency.
Drawings
The present invention is further explained by using the attached drawings, but the content in the attached drawings does not constitute any limitation to the present invention.
FIG. 1 is a schematic view of the present invention;
FIG. 2 is a schematic view of the structure of the continuous filtration apparatus.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and examples.
Example 1
The utility model provides a protein purification device, includes fermentation vat 1, first liquid pump 2, ultrasonication device 3, continuous filter equipment 4, buffer tank 5, second liquid pump 6, first rotary device 7, porous resin column frame 8, spacing ring 9, adsorb station seat hole 10, elution station seat hole 11, wash station seat hole 12, porous resin adsorption column 13, eluent pipe 14, eluent case 15, washing liquid pipe 16, deionized water case 17 filters former liquid pipe 19, flowmeter 20 and connects liquid basin 21.
The fermentation tank 1 is communicated with the bottom of an ultrasonic crushing device 3 through a first liquid pump 2, fermented bacteria liquid is conveyed to the ultrasonic crushing device 3, thalli in the bacteria liquid are subjected to ultrasonic crushing through the ultrasonic crushing device 3 and then conveyed to a continuous filtering device 4, the filtered clear liquid is conveyed to a buffer tank 5 through the continuous filtering device 4 and then conveyed to a porous resin adsorption column 13 of an adsorption station seat hole 10 in a continuous purifying mechanism to adsorb required protein in the clear liquid, after the introduced clear liquid reaches a preset amount, a first rotating device 7 rotates to enable the porous resin adsorption column 13 to reach a station of a next elution station seat hole 11, then eluent is injected into the porous resin adsorption column 13, the adsorbed protein is eluted and pulled down and collected, after the preset eluent is injected, the first rotating device 7 rotates again, deionized water is injected into the porous resin adsorption column 13, the residual eluent is cleaned, and then the residual eluent returns to the adsorption station seat hole 10. Thus realizing the continuous adsorption, elution and cleaning of the adsorption column of the protein. And then the collected protein eluent is counted into alcohol precipitation to obtain purified protein. This allows for continuous purification of the protein.
Wherein, the continuous purification mechanism can be a plurality of mechanisms to match the filtration capacity of the continuous filtration device 4.
Wherein, the ultrasonic crushing device 3 comprises a crushing pipe 31, and an ultrasonic generator 32 is arranged along the length direction of the crushing pipe 31. The crushing pipe 31 is provided with a spiral fan blade 33 to increase the stroke of the filtrate therein and improve the crushing rate.
Wherein, the continuous filtering device 4 comprises a V-shaped rotary drum 41, the middle part of the rotary drum 41 is a porous filtering area 42, and the bottom of the rotary drum 41 is connected with a second rotating device 43; a filtrate collecting shell 44 is covered on the periphery of the rotary drum 41, and the inner wall of the filtrate collecting shell 44 is connected with a second rotating device 43 through a support rod 45; the bottom of the filtrate collecting shell 44 is communicated with the buffer tank 5; a filter residue collecting shell 46 is arranged on the outer cover of the filtrate collecting shell 44, a retaining ring 47 matched with the top of the rotary drum 41 is formed in the top of the filter residue collecting shell 46 in a protruding mode, a discharge hole 48 is formed in the bottom of the filter residue collecting shell 46, and the filter residue collecting shell 46 is connected with the filtrate collecting shell 44 through a support rod 49.
When the crushed bacteria liquid entering the V-shaped rotary drum 41 passes through the porous filtering area 42, clear liquid is thrown out to enter the filtrate collecting shell 44 and then sent to the buffer tank 5, and the crushed bacteria wall, organelles and the like continuously move upwards along the outer wall of the rotary drum 41 and are thrown into the filter residue collecting shell 46 to be collected, so that continuous solid-liquid separation is realized.
The flow meters 20 are installed on the eluent tube 14, the cleaning liquid tube 16 and the raw filtration tube 19 for flow control, and the respective devices can be automatically controlled by a PLC controller or a computer.
The first rotating device 7 is a motor, and the second rotating device 43 is a rotary cylinder or a servo motor.
Liquid receiving basins 21 are respectively arranged below the porous resin adsorption columns 13 of the adsorption station seat hole 10, the elution station seat hole 11 and the cleaning station seat hole 12; a material basin 410 is placed below the filtrate collection shell 44.
It should be finally noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit the protection scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced with equivalents without departing from the spirit and scope of the technical solutions of the present invention.
Claims (8)
1. A protein purification device is characterized by comprising a fermentation tank (1), wherein the fermentation tank (1) is communicated with the bottom of an ultrasonic crushing device (3) through a first liquid pump (2), a continuous filtering device (4) is arranged below a liquid outlet at the upper part of the ultrasonic crushing device (3), the continuous filtering device (4) is communicated with a buffer tank (5), the buffer tank (5) is respectively communicated with a plurality of continuous purification mechanisms through a plurality of filtering raw liquid pipes (19), and a second liquid pump (6) is arranged on the filtering raw liquid pipes (19); the continuous purification mechanism comprises a first rotating device (7), the first rotating device (7) is connected with a porous resin column frame (8), and an adsorption station seat hole (10), an elution station seat hole (11) and a cleaning station seat hole (12) are sequentially connected to the porous resin column frame (8) along the circumferential direction; porous resin adsorption columns (13) are fixed on the adsorption station seat hole (10), the elution station seat hole (11) and the cleaning station seat hole (12); an elution liquid pipe (14) is arranged above the elution station seat hole (11), and an elution liquid box (15) is communicated above the elution liquid pipe (14); a cleaning liquid pipe (16) is arranged above the cleaning station seat hole (12), and the cleaning liquid pipe (16) is communicated with a deionized water tank (17); solenoid valves (18) are respectively arranged on the eluent tube (14) and the cleaning liquid tube (16); a filtering raw liquid pipe (19) is arranged above the adsorption station seat hole (10).
2. The protein purification apparatus as claimed in claim 1, wherein the ultrasonic disruption means (3) comprises a disruption tube (31), and an ultrasonic generator (32) is installed along the length of the disruption tube (31).
3. The protein purification apparatus as claimed in claim 1, wherein a spiral fan blade (33) is installed in the crushing tube (31).
4. The protein purification apparatus as claimed in claim 1, wherein the continuous filtration device (4) comprises a V-shaped drum (41), the middle of the drum (41) is a porous filtration zone (42), and the bottom of the drum (41) is connected with a second rotating device (43); a filtrate collecting shell (44) is arranged on the periphery of the rotary drum (41) in a covering mode, and the inner wall of the filtrate collecting shell (44) is connected with a second rotating device (43) through a supporting rod (45); the bottom of the filtrate collecting shell (44) is communicated with the buffer tank (5); the filter residue collecting shell (44) is provided with a filter residue collecting shell (46) in a covering mode, a retaining ring (47) matched with the top of the rotary drum (41) is formed in the top of the filter residue collecting shell (46) in a protruding mode, a discharge hole (48) is formed in the bottom of the filter residue collecting shell (46), and the filter residue collecting shell (46) is connected with the filter residue collecting shell (44) through a support rod (49).
5. The protein purification apparatus as claimed in claim 4, wherein flow meters (20) are installed on the elution liquid pipe (14), the cleaning liquid pipe (16) and the filtration raw liquid pipe (19).
6. The protein purification apparatus according to claim 4, wherein the first rotation means (7) is a motor and the second rotation means (43) is a rotary cylinder or a servo motor.
7. The protein purification apparatus according to claim 4, wherein a liquid receiving basin (21) is disposed under the porous resin adsorption column (13) of the adsorption station seat hole (10), the elution station seat hole (11), and the washing station seat hole (12); a material basin is arranged below the filtrate collection shell (44).
8. The protein purification apparatus as claimed in any one of claims 1 to 7, wherein a limiting ring (9) is protruded at the center of the porous resin adsorption column (13).
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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CN202221639177 | 2022-06-29 | ||
CN2022216391772 | 2022-06-29 |
Publications (1)
Publication Number | Publication Date |
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CN218115337U true CN218115337U (en) | 2022-12-23 |
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CN202222663837.7U Active CN218115337U (en) | 2022-06-29 | 2022-10-10 | Protein purification device |
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CN (1) | CN218115337U (en) |
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2022
- 2022-10-10 CN CN202222663837.7U patent/CN218115337U/en active Active
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